TISIDB

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm
Domain	PF03987 Autophagocytosis associated protein PF10381 Autophagocytosis associated protein C-terminal PF03986 Autophagocytosis associated protein (Atg3)
Function	E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

> Gene Ontology [ TOP ]
Biological Process	GO:0000045 autophagosome assembly GO:0000422 mitophagy GO:0006612 protein targeting to membrane GO:0006909 phagocytosis GO:0006914 autophagy GO:0007033 vacuole organization GO:0008637 apoptotic mitochondrial changes GO:0010927 cellular component assembly involved in morphogenesis GO:0016236 macroautophagy GO:0022604 regulation of cell morphogenesis GO:0030031 cell projection assembly GO:0030100 regulation of endocytosis GO:0042384 cilium assembly GO:0043653 mitochondrial fragmentation involved in apoptotic process GO:0044782 cilium organization GO:0044804 nucleophagy GO:0045806 negative regulation of endocytosis GO:0050764 regulation of phagocytosis GO:0050765 negative regulation of phagocytosis GO:0051051 negative regulation of transport GO:0060271 cilium morphogenesis GO:0060491 regulation of cell projection assembly GO:0060627 regulation of vesicle-mediated transport GO:0061726 mitochondrion disassembly GO:0072657 protein localization to membrane GO:0090150 establishment of protein localization to membrane GO:1902017 regulation of cilium assembly GO:1902115 regulation of organelle assembly GO:1903008 organelle disassembly GO:1905037 autophagosome organization
Molecular Function	GO:0016874 ligase activity GO:0019776 Atg8 ligase activity GO:0019777 Atg12 transferase activity GO:0019787 ubiquitin-like protein transferase activity
Cellular Component	GO:0000151 ubiquitin ligase complex GO:0000153 cytoplasmic ubiquitin ligase complex

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa04140 Regulation of autophagy
Reactome	R-HSA-2262752: Cellular responses to stress R-HSA-1632852: Macroautophagy

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between ATG3 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of ATG3 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	logFC: -0.85; FDR: 0.04630	Resistant to T cell-mediated killing
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the expression difference of ATG3 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	-0.092	0.628
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.12	0.962
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.071	0.969
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	-0.177	0.524
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	-0.241	0.877
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	-0.095	0.962
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	-0.102	0.804
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	-0.007	0.997
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	-0.187	0.92
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	0.192	0.911
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.21	0.938
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.096	0.115

> Mutation difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the mutation difference of ATG3 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	0	0	1
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	0	0	1
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	0	0	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	11.1	11.1	0.36
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	25	25	0.364
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	0	0	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	0	0	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	1

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of ATG3. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of ATG3. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by ATG3. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of ATG3. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of ATG3 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between ATG3 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	ATG3
Name	autophagy related 3
Aliases	PC3-96; FLJ22125; MGC15201; DKFZp564M1178; APG3L; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy rela ......
Chromosomal Location	3q13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting ATG3 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse ATG3