Cancer

breast cancer

Regulation

downregulation

Reporting

To shed light on their roles of miR-211 in breast cancer the expression of miR-211 was examined by real-time RT-PCR in breast cancer and normal tissues. MiR-211 is significantly downregulated in breast cancer

Pubmed

25680404

Method

qPCR

Cancer

colon cancer

Regulation

upregulation

Reporting

Further later p-TNM hazard ratio HR=2.973 95% confidence interval CI: 1.329-6.519 P=0.003 and high expression of miR-211 HR=2.394 95% CI: 1.210-4.910 P=0.006 were identified as risk factors for colon cancer prognosis

Pubmed

25932237

Method

Cancer

colorectal cancer

Regulation

upregulation

Reporting

MicroRNA 211 expression is upregulated and associated with poor prognosis in colorectal cancer: a case control study. The role of up/downregulation microRNA-211 miR-211 during human tumorigenesis is still contentious and may exhibit tissue-specific regulatory manner but the exhaustive mechanisms underlying its pro/anti-oncogenic effects remain to be unknown. The expression level of miR-211 in matched normal and tumor tissues of CRC group and healthy group was evaluated using a quantitative real-time RT-PCR qRT-PCR. Based on the average miR-211 levels two groups of low or high expression were formed in CRC group. No statistically significant differences were found in miR-211 levels among tumorous and normal tissues of CRC patient group P = 0.59. Also no statistically significant correlation was determined between clinicopathological factors and miR-211 expression level in CRC group. However miR-211 expression levels between the CRC group and the healthy group were determined to be of statistical significance P < 0.0001. There were 33 50 % CRC patients that expressed low levels of miR-211 and 33 50 % CRC patients that expressed high levels of miR-211. A median survival between low levels of miR-211 group and high levels of miR-211 group was evaluated via Kaplan-Meier and the difference was of statistical significance P = 0.035. The univariate analysis of the factors that may affect survival indicated invasion depth P = 0.063 lymphovascular invasion P = 0.011 perineural invasion P = 0.009 and miR-211 expression level P = 0.041 presence to be effective. In the multivariate analysis of these factors with overall survival only miR-211 expression level P = 0.01 was effective on overall survival. Our results suggest for the first time that miR-211 expressed more in CRC patients than in healthy group could be a new prognostic biomarker in order to predict survival

Pubmed

26152286

Method

qPCR

Cancer

gastric cancer

Regulation

downregulation

Reporting

Deregulation of miR-211 has been observed in various types of human cancers. The expression of miR-211 in GC was detected by using quantitative real-time PCR qRT-PCR. A luciferase reporter assay and western blot were performed to confirm the target gene of miR-211. MiR-211 was significantly down-regulated in GC. MiR-211 might be a potential therapeutic target for GC treatment in the future

Pubmed

26823713

Method

qPCR

Cancer

head and neck cancer

Regulation

deregulation

Reporting

miR 211 promotes the progression of head and neck carcinomas by targeting TGFβRII. miR-211 up-regulation and transforming growth factor-β type II receptor TGFβRII down-regulation are associated with poor prognosis of head and neck squamous cell carcinoma HNSCC miR-211 directly targets TGFβRII with the miR-211-TGFβRII-c-Myc axis promoting HNSCC progression. An inverse correlation of miR-211 and TGFβRII expression was found in metastatic HNSCC samples

Pubmed

23726841

Method

Cancer

lung squamous cell cancer

Regulation

upregulation

Reporting

In the current study qRT-PCR was performed to measure miR-211 expression in human non-small cell lung cancer NSCLC cell lines and tissues. We used luciferase reporter assay to find the potential target of miR-211. We found that miR-211 expression was upregulated in human non-small cell lung cancer NSCLC cell lines and tissues. The overexpression of miR-211 enhanced NSCLC cell proliferation colony formation and invasion

Pubmed

26277787

Method

qPCR

Cancer

melanoma

Regulation

downregulation

Reporting

An exploratory miRNA analysis of 666 miRs by low density microRNA arrays was conducted on formalin fixed and paraffin embedded tissues FFPE from 10 older adults and 10 young adults including conventional melanoma and melanocytic neoplasms of uncertain biological significance. MiR-211 was dramatically downregulated compared to nevi controls decreased with increasing age and was among the miRs linked to metastatic processes

Pubmed

20302635

Method

Microarray

Cancer

melanoma

Regulation

downregulation

Reporting

Differential expression of microRNAs during melanoma progression: miR 200c miR 205 and miR 211 are downregulated in melanoma and act as tumour suppressors. To identify improved molecular markers to support histological examination we used microarray analysis of formalin-fixed and paraffin-embedded samples from different stages of melanomagenesis to identify differentially expressed microRNAs miRNAs. We have identified a series of differentially expressed miRNAs that could be useful as diagnostic or prognostic markers for melanoma and have shown that three miRNAs namely miR-200c miR-205 and miR-211 act as tumour suppressors

Pubmed

22223089

Method

Microarray

Cancer

ovarian cancer

Regulation

downregulation

Reporting

miR-211 is known to be dysregulated in ovarian cancer: however its function and the downstream effect of its loss-of-function in ovarian cancer have not been described before. We analyzed miR-211 expression in clinical samples of primary EOC tissues compared to normal epithelial ovarian tissues and in the EOC cell lines: OVCAR3 Caov3 OVCA429 SKOV3 and A2780 compared to human ovarian surface epithelial cells. We then investigated the effect of miR-211 on EOC cell proliferation and apoptosis by counting cell numbers MTT colony formation cell cycle and PI/Annexin V staining assays. A luciferase reporter system was developed to assess miR-211 regulation of the predicted targets. Expression level of discovered targets and correlation with miR-211 expression were analyzed in EOC tissues. We found that the expression of miR-211 is downregulated in EOC tissues and cell lines compared to normal epithelial ovarian tissue and human ovarian surface epithelial cells respectively

Pubmed

25889927

Method

Cancer

pancreatic cancer

Regulation

upregulation

Reporting

In a high-throughput microRNA miRNA array miR-211 emerged as the best discriminating miRNA with high expression associated with long survival. In particular S2-028 with lower metastatic ability had a higher expression of miR-211 compared to the S2-007 with higher metastatic capacity

Pubmed

24940696

Method

Microarray