This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are
presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.
"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.
Num | microRNA | Gene | miRNA log2FC | miRNA pvalue | Gene log2FC | Gene pvalue | Interaction | Correlation beta | Correlation P-value | PMID | Reported in cancer studies |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | hsa-miR-155-5p | ACTA1 | -0.32 | 0.67318 | 0.68 | 0.22496 | MirTarget; miRNATAP | -0.31 | 0.00084 | NA | |
2 | hsa-miR-155-5p | AHRR | -0.32 | 0.67318 | -0.45 | 0.15751 | MirTarget | -0.13 | 0.02385 | NA | |
3 | hsa-miR-155-5p | ANO5 | -0.32 | 0.67318 | -0.52 | 0.23338 | MirTarget | -0.31 | 0.00017 | NA | |
4 | hsa-miR-155-5p | ARL10 | -0.32 | 0.67318 | -0.39 | 0.12807 | miRNAWalker2 validate | -0.13 | 0.00767 | NA | |
5 | hsa-miR-155-5p | ATRNL1 | -0.32 | 0.67318 | 0.32 | 0.59427 | MirTarget; mirMAP | -0.79 | 0 | NA | |
6 | hsa-miR-155-5p | BDNF | -0.32 | 0.67318 | -0.53 | 0.1379 | miRNATAP | -0.15 | 0.02622 | NA | |
7 | hsa-miR-155-5p | C1QL2 | -0.32 | 0.67318 | -0.86 | 0.18725 | miRNATAP | -0.26 | 0.01554 | NA | |
8 | hsa-miR-155-5p | CAB39L | -0.32 | 0.67318 | 0.46 | 0.20249 | miRNAWalker2 validate | -0.12 | 0.00272 | NA | |
9 | hsa-miR-155-5p | CARD10 | -0.32 | 0.67318 | -0.35 | 0.4043 | MirTarget | -0.15 | 0.00236 | NA | |
10 | hsa-miR-155-5p | CCND1 | -0.32 | 0.67318 | -0.04 | 0.9587 | miRNAWalker2 validate | -0.22 | 0.00251 | 26955820 | MicroRNA 155 expression inversely correlates with pathologic stage of gastric cancer and it inhibits gastric cancer cell growth by targeting cyclin D1 |
11 | hsa-miR-155-5p | CHST9 | -0.32 | 0.67318 | -0.11 | 0.87995 | miRNATAP | -0.41 | 0.00277 | NA | |
12 | hsa-miR-155-5p | CLDN8 | -0.32 | 0.67318 | 0.69 | 0.35896 | MirTarget | -0.6 | 0 | NA | |
13 | hsa-miR-155-5p | CNTN3 | -0.32 | 0.67318 | 0.64 | 0.28014 | MirTarget | -0.32 | 0.00396 | NA | |
14 | hsa-miR-155-5p | DACH2 | -0.32 | 0.67318 | -1.88 | 0.01349 | mirMAP | -0.35 | 0.00821 | NA | |
15 | hsa-miR-155-5p | DYNC1I1 | -0.32 | 0.67318 | 0.74 | 0.05878 | MirTarget; miRNATAP | -0.22 | 0.0042 | NA | |
16 | hsa-miR-155-5p | DYNC2H1 | -0.32 | 0.67318 | 0.44 | 0.22236 | miRNAWalker2 validate | -0.11 | 0.01069 | NA | |
17 | hsa-miR-155-5p | EEF1A2 | -0.32 | 0.67318 | 0.53 | 0.44481 | miRNAWalker2 validate | -0.39 | 0.00372 | NA | |
18 | hsa-miR-155-5p | ENTPD3 | -0.32 | 0.67318 | 0.62 | 0.14444 | MirTarget | -0.15 | 0.04652 | NA | |
19 | hsa-miR-155-5p | ETNK2 | -0.32 | 0.67318 | -0.06 | 0.88513 | miRNATAP | -0.17 | 7.0E-5 | NA | |
20 | hsa-miR-155-5p | FGF9 | -0.32 | 0.67318 | -0.06 | 0.91069 | miRNATAP | -0.61 | 0 | NA | |
21 | hsa-miR-155-5p | FIGN | -0.32 | 0.67318 | 0.05 | 0.91019 | mirMAP | -0.14 | 0.04482 | NA | |
22 | hsa-miR-155-5p | FLRT2 | -0.32 | 0.67318 | 0.39 | 0.40703 | mirMAP | -0.16 | 0.02724 | NA | |
23 | hsa-miR-155-5p | FOXP4 | -0.32 | 0.67318 | -0.27 | 0.69437 | mirMAP | -0.1 | 0.02846 | NA | |
24 | hsa-miR-155-5p | GATM | -0.32 | 0.67318 | 0.1 | 0.82038 | miRNAWalker2 validate | -0.19 | 0.00021 | NA | |
25 | hsa-miR-155-5p | GDPD1 | -0.32 | 0.67318 | 0.07 | 0.78445 | MirTarget | -0.18 | 0.00041 | NA | |
26 | hsa-miR-155-5p | GNAS | -0.32 | 0.67318 | 0.13 | 0.89141 | miRNAWalker2 validate; MirTarget | -0.13 | 0 | NA | |
27 | hsa-miR-155-5p | GRIP1 | -0.32 | 0.67318 | -0.35 | 0.35973 | miRNATAP | -0.17 | 0.00447 | NA | |
28 | hsa-miR-155-5p | HSPA4L | -0.32 | 0.67318 | 0.17 | 0.62992 | miRNAWalker2 validate | -0.21 | 0.00181 | NA | |
29 | hsa-miR-155-5p | IGDCC4 | -0.32 | 0.67318 | -0.2 | 0.59831 | MirTarget | -0.34 | 0 | NA | |
30 | hsa-miR-155-5p | IGF1R | -0.32 | 0.67318 | 0.18 | 0.77996 | mirMAP | -0.13 | 0.01272 | NA | |
31 | hsa-miR-155-5p | IL17RD | -0.32 | 0.67318 | -0.08 | 0.87096 | mirMAP | -0.14 | 0.00955 | NA | |
32 | hsa-miR-155-5p | INA | -0.32 | 0.67318 | -0.39 | 0.48595 | miRNAWalker2 validate | -0.24 | 0.02626 | NA | |
33 | hsa-miR-155-5p | KDM5B | -0.32 | 0.67318 | 0.05 | 0.93707 | MirTarget | -0.14 | 2.0E-5 | NA | |
34 | hsa-miR-155-5p | KRAS | -0.32 | 0.67318 | 0.01 | 0.98017 | miRNAWalker2 validate; miRNATAP | -0.12 | 0.00602 | NA | |
35 | hsa-miR-155-5p | LCA5 | -0.32 | 0.67318 | 0.21 | 0.51761 | MirTarget | -0.14 | 7.0E-5 | NA | |
36 | hsa-miR-155-5p | MAP4K3 | -0.32 | 0.67318 | 0.24 | 0.61905 | MirTarget | -0.11 | 0 | NA | |
37 | hsa-miR-155-5p | MED12L | -0.32 | 0.67318 | 0.22 | 0.56175 | miRNATAP | -0.18 | 0.01314 | NA | |
38 | hsa-miR-155-5p | MEST | -0.32 | 0.67318 | 0.06 | 0.93011 | miRNAWalker2 validate | -0.21 | 0.00018 | NA | |
39 | hsa-miR-155-5p | MEX3B | -0.32 | 0.67318 | 0.06 | 0.85358 | miRNATAP | -0.22 | 2.0E-5 | NA | |
40 | hsa-miR-155-5p | MMP16 | -0.32 | 0.67318 | -0.38 | 0.29717 | mirMAP | -0.15 | 0.0288 | NA | |
41 | hsa-miR-155-5p | MPP2 | -0.32 | 0.67318 | 0.09 | 0.80743 | miRNAWalker2 validate | -0.2 | 0.00096 | NA | |
42 | hsa-miR-155-5p | MSI2 | -0.32 | 0.67318 | -0.08 | 0.82643 | miRNAWalker2 validate | -0.11 | 0.00025 | NA | |
43 | hsa-miR-155-5p | NES | -0.32 | 0.67318 | -0.32 | 0.52183 | miRNAWalker2 validate | -0.16 | 0.00127 | NA | |
44 | hsa-miR-155-5p | NOVA1 | -0.32 | 0.67318 | 0.11 | 0.80837 | miRNAWalker2 validate; miRNATAP | -0.44 | 0 | NA | |
45 | hsa-miR-155-5p | NR2F2 | -0.32 | 0.67318 | -0.16 | 0.78608 | miRNATAP | -0.1 | 0.00637 | NA | |
46 | hsa-miR-155-5p | OGN | -0.32 | 0.67318 | 0.98 | 0.07787 | miRNATAP | -0.24 | 0.01663 | NA | |
47 | hsa-miR-155-5p | PACSIN3 | -0.32 | 0.67318 | -0.07 | 0.89105 | miRNAWalker2 validate | -0.18 | 4.0E-5 | NA | |
48 | hsa-miR-155-5p | PANK1 | -0.32 | 0.67318 | -0.03 | 0.94547 | miRNATAP | -0.11 | 0.00374 | NA | |
49 | hsa-miR-155-5p | PEG3 | -0.32 | 0.67318 | -0.86 | 0.17776 | miRNATAP | -0.57 | 0 | NA | |
50 | hsa-miR-155-5p | PGAP1 | -0.32 | 0.67318 | -0.03 | 0.93261 | mirMAP | -0.14 | 0.00032 | NA | |
51 | hsa-miR-155-5p | PGR | -0.32 | 0.67318 | 0.81 | 0.19558 | mirMAP | -0.32 | 0.0058 | 20388420; 19454029; 23162645 | It's indicated that the up-regulation of miR-155 expression was associated with advanced TNM clinical stage median 0.316 0.358 and 0.417 respectively for stage I II and III tumor P = 0.002 lymph node metastasis median 0.383 and 0.355 respectively for cases with positive and negative lymph nodes P = 0.034 higher proliferation index median 0.387 and 0.353 respectively for cases with high proliferation index Ki67 > 10% and low proliferation index Ki67 ≤ 10% P = 0.019 estrogen receptor-positive 0.367 and 0.318 respectively for cases with positive estrogen receptor and negative group P = 0.041 and progesterone receptor-positive 0.398 and 0.335 respectively for cases with positive progesterone receptor and negative group P = 0.029 in patients with breast cancer; The expression of miR-155 is up-regulated in primary breast cancer especially in patients with positive estrogen and progesterone receptor;While the expression of all three miRNAs was similar in samples from healthy women compared to those with breast cancer women with progesterone receptor PR p = 0.016 positive tumors had higher miR-155 expression than tumors that were negative for these receptors;miR 155 and miR 31 are differentially expressed in breast cancer patients and are correlated with the estrogen receptor and progesterone receptor status; The expression levels of miR-155 but not miR-31 were inversely correlated with estrogen receptor ER and progesterone receptor PR expression ER r=-0.353 P=0.003; PR r=-0.357 P=0.003 |
52 | hsa-miR-155-5p | PLEKHA5 | -0.32 | 0.67318 | -0.08 | 0.85824 | miRNAWalker2 validate | -0.1 | 0.00335 | NA | |
53 | hsa-miR-155-5p | PPM1L | -0.32 | 0.67318 | 0.06 | 0.83366 | mirMAP | -0.15 | 0.00746 | NA | |
54 | hsa-miR-155-5p | PRRG3 | -0.32 | 0.67318 | -0.44 | 0.46451 | mirMAP | -0.32 | 0.00232 | NA | |
55 | hsa-miR-155-5p | RAB11FIP2 | -0.32 | 0.67318 | 0.41 | 0.39042 | miRNAWalker2 validate; MirTarget; miRNATAP | -0.16 | 0 | NA | |
56 | hsa-miR-155-5p | RAPGEF4 | -0.32 | 0.67318 | -0.45 | 0.07612 | miRNATAP | -0.12 | 0.00342 | NA | |
57 | hsa-miR-155-5p | SALL1 | -0.32 | 0.67318 | 0.28 | 0.65757 | miRNATAP | -0.61 | 0 | NA | |
58 | hsa-miR-155-5p | SATB1 | -0.32 | 0.67318 | 0.17 | 0.72112 | MirTarget; miRNATAP | -0.22 | 0.00029 | NA | |
59 | hsa-miR-155-5p | SCML2 | -0.32 | 0.67318 | -0.13 | 0.67403 | mirMAP | -0.13 | 0.00687 | NA | |
60 | hsa-miR-155-5p | SEC14L5 | -0.32 | 0.67318 | 0.04 | 0.90663 | miRNATAP | -0.36 | 0 | NA | |
61 | hsa-miR-155-5p | SH3BP4 | -0.32 | 0.67318 | -0.06 | 0.92912 | miRNAWalker2 validate | -0.1 | 0.01691 | NA | |
62 | hsa-miR-155-5p | SIM2 | -0.32 | 0.67318 | -0.52 | 0.30039 | miRNATAP | -0.3 | 0.00182 | NA | |
63 | hsa-miR-155-5p | SLC20A2 | -0.32 | 0.67318 | -0 | 0.99716 | miRNATAP | -0.1 | 0.0009 | NA | |
64 | hsa-miR-155-5p | SMAD9 | -0.32 | 0.67318 | -0.23 | 0.49449 | mirMAP | -0.2 | 0.00137 | NA | |
65 | hsa-miR-155-5p | SNED1 | -0.32 | 0.67318 | -0.26 | 0.57987 | mirMAP | -0.17 | 0.00139 | NA | |
66 | hsa-miR-155-5p | SORCS1 | -0.32 | 0.67318 | 1.3 | 0.10393 | MirTarget | -0.37 | 0.0168 | NA | |
67 | hsa-miR-155-5p | SOX11 | -0.32 | 0.67318 | 0.94 | 0.26156 | miRNATAP | -0.55 | 0.00079 | NA | |
68 | hsa-miR-155-5p | SOX6 | -0.32 | 0.67318 | -0.35 | 0.4587 | miRTarBase; miRNATAP | -0.19 | 0.0116 | 21989846 | Ectopic expression of sex-determining region Y box 6 SOX6 was able to reverse the growth-promoting property of miR-155; Concordantly the results demonstrated for the first time that SOX6 is a direct target of miR-155; In addition a decline in p21waf1/cip1 expression caused by miR-155 could be reversed by SOX6 expression; The current data indicated that SOX6 is a novel target of miR-155 and that miR-155 enhances liver cell tumorigenesis at least in part through the novel miR-155/SOX6/p21waf1/cip1 axis |
69 | hsa-miR-155-5p | SOX9 | -0.32 | 0.67318 | 0.34 | 0.57504 | miRNATAP | -0.14 | 0.01283 | NA | |
70 | hsa-miR-155-5p | ST8SIA3 | -0.32 | 0.67318 | 0.41 | 0.59717 | mirMAP | -0.31 | 0.01334 | NA | |
71 | hsa-miR-155-5p | STOX2 | -0.32 | 0.67318 | -0.22 | 0.44405 | mirMAP | -0.2 | 4.0E-5 | NA | |
72 | hsa-miR-155-5p | STXBP5L | -0.32 | 0.67318 | -0.84 | 0.07835 | miRNATAP | -0.18 | 0.03581 | NA | |
73 | hsa-miR-155-5p | TMEM132B | -0.32 | 0.67318 | -0.11 | 0.83184 | mirMAP | -0.23 | 0.0199 | NA | |
74 | hsa-miR-155-5p | TRIM2 | -0.32 | 0.67318 | 0.13 | 0.80027 | miRNATAP | -0.18 | 0.00011 | NA | |
75 | hsa-miR-155-5p | TSPAN5 | -0.32 | 0.67318 | -0.13 | 0.66861 | MirTarget | -0.12 | 0.01853 | NA | |
76 | hsa-miR-155-5p | UGDH | -0.32 | 0.67318 | 0.19 | 0.69988 | miRNAWalker2 validate | -0.1 | 0.0001 | NA | |
77 | hsa-miR-155-5p | UNC80 | -0.32 | 0.67318 | -0.2 | 0.71626 | MirTarget | -0.53 | 0 | NA | |
78 | hsa-miR-155-5p | VAV2 | -0.32 | 0.67318 | -0.41 | 0.42761 | miRNAWalker2 validate | -0.15 | 0.00052 | NA | |
79 | hsa-miR-155-5p | WNT5A | -0.32 | 0.67318 | 0.21 | 0.67651 | miRNAWalker2 validate; mirMAP | -0.15 | 0.00991 | NA | |
80 | hsa-miR-155-5p | ZC3H12B | -0.32 | 0.67318 | -0.36 | 0.219 | mirMAP | -0.12 | 0.02744 | NA | |
81 | hsa-miR-155-5p | ZNF518B | -0.32 | 0.67318 | -0.36 | 0.43527 | MirTarget; miRNATAP | -0.16 | 0.00108 | NA | |
82 | hsa-miR-155-5p | ZNF677 | -0.32 | 0.67318 | -0.07 | 0.79759 | mirMAP | -0.12 | 0.00737 | NA | |
83 | hsa-miR-155-5p | ZNF709 | -0.32 | 0.67318 | 0.17 | 0.64315 | mirMAP | -0.16 | 0.0002 | NA | |
84 | hsa-miR-155-5p | ZNF880 | -0.32 | 0.67318 | -0.38 | 0.27286 | MirTarget | -0.11 | 0.0132 | NA |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | EMBRYO DEVELOPMENT | 16 | 894 | 8.168e-07 | 0.001267 |
2 | APPENDAGE DEVELOPMENT | 8 | 169 | 5.561e-07 | 0.001267 |
3 | LIMB DEVELOPMENT | 8 | 169 | 5.561e-07 | 0.001267 |
4 | DEVELOPMENTAL GROWTH INVOLVED IN MORPHOGENESIS | 6 | 104 | 5.003e-06 | 0.00582 |
5 | REPRODUCTIVE SYSTEM DEVELOPMENT | 10 | 408 | 8.047e-06 | 0.007488 |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | NUCLEIC ACID BINDING TRANSCRIPTION FACTOR ACTIVITY | 18 | 1199 | 1.915e-06 | 0.001779 |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|
Num | Pathway | Pathview | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|---|
1 | Rap1_signaling_pathway_hsa04015 | 6 | 206 | 0.0002314 | 0.01203 | |
2 | cAMP_signaling_pathway_hsa04024 | 5 | 198 | 0.001482 | 0.03854 | |
3 | Signaling_pathways_regulating_pluripotency_of_stem_cells_hsa04550 | 4 | 139 | 0.002805 | 0.04861 | |
4 | mTOR_signaling_pathway_hsa04150 | 4 | 151 | 0.003774 | 0.04906 | |
5 | MAPK_signaling_pathway_hsa04010 | 5 | 295 | 0.008076 | 0.08399 | |
6 | AMPK_signaling_pathway_hsa04152 | 3 | 121 | 0.01443 | 0.1049 | |
7 | PI3K_Akt_signaling_pathway_hsa04151 | 5 | 352 | 0.01634 | 0.1049 | |
8 | Ras_signaling_pathway_hsa04014 | 4 | 232 | 0.01653 | 0.1049 | |
9 | FoxO_signaling_pathway_hsa04068 | 3 | 132 | 0.01816 | 0.1049 | |
10 | Phospholipase_D_signaling_pathway_hsa04072 | 3 | 146 | 0.02363 | 0.1229 | |
11 | Focal_adhesion_hsa04510 | 3 | 199 | 0.05145 | 0.229 | |
12 | Gap_junction_hsa04540 | 2 | 88 | 0.05289 | 0.229 | |
13 | Regulation_of_actin_cytoskeleton_hsa04810 | 3 | 208 | 0.05726 | 0.229 | |
14 | Oocyte_meiosis_hsa04114 | 2 | 124 | 0.0958 | 0.3503 | |
15 | Autophagy_animal_hsa04140 | 2 | 128 | 0.101 | 0.3503 | |
16 | Apelin_signaling_pathway_hsa04371 | 2 | 137 | 0.1131 | 0.3676 | |
17 | Wnt_signaling_pathway_hsa04310 | 2 | 146 | 0.1255 | 0.3744 | |
18 | Phagosome_hsa04145 | 2 | 152 | 0.134 | 0.3744 | |
19 | Hippo_signaling_pathway_hsa04390 | 2 | 154 | 0.1368 | 0.3744 | |
20 | Cellular_senescence_hsa04218 | 2 | 160 | 0.1454 | 0.3781 | |
21 | Tight_junction_hsa04530 | 2 | 170 | 0.16 | 0.3963 | |
22 | Endocytosis_hsa04144 | 2 | 244 | 0.2734 | 0.5266 |