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This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.

"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.

miRNA-gene regulations

(Download full result)

Num microRNA           Gene miRNA log2FC miRNA pvalue Gene log2FC Gene pvalue Interaction Correlation beta Correlation P-value PMID Reported in cancer studies
1 hsa-miR-29b-3p ABCB6 0.18 0.08561 0.1 0.06312 MirTarget -0.13 0 NA
2 hsa-miR-29b-3p ABL1 0.18 0.08561 -0.01 0.80282 miRNATAP -0.18 0 NA
3 hsa-miR-29b-3p ABL2 0.18 0.08561 0.12 0.01311 mirMAP -0.13 0 NA
4 hsa-miR-29b-3p ADAM22 0.18 0.08561 0.22 0.04639 miRNATAP -0.21 4.0E-5 NA
5 hsa-miR-29b-3p ADAMTS10 0.18 0.08561 -0.06 0.34973 MirTarget; miRNATAP -0.13 1.0E-5 NA
6 hsa-miR-29b-3p ADAMTS17 0.18 0.08561 0.21 0.03129 MirTarget; miRNATAP -0.18 8.0E-5 NA
7 hsa-miR-29b-3p ADAMTS6 0.18 0.08561 -0.12 0.24023 MirTarget; miRNATAP -0.49 0 NA
8 hsa-miR-29b-3p ADAMTS7 0.18 0.08561 -0.77 0 MirTarget; miRNATAP -0.23 0.00014 NA
9 hsa-miR-29b-3p ADAMTS9 0.18 0.08561 0.11 0.31553 miRNATAP -0.25 0 NA
10 hsa-miR-29b-3p ADCYAP1R1 0.18 0.08561 0.8 0 miRNATAP -0.19 0.00635 NA
11 hsa-miR-29b-3p ADM2 0.18 0.08561 -0.69 0 mirMAP -0.16 0.01332 NA
12 hsa-miR-29b-3p AFF4 0.18 0.08561 0.05 0.41457 miRNATAP -0.11 0 NA
13 hsa-miR-29b-3p AKAP13 0.18 0.08561 -0.09 0.14298 MirTarget; miRNATAP -0.13 0 NA
14 hsa-miR-29b-3p AMMECR1 0.18 0.08561 -0.11 0.0201 miRNATAP -0.16 0 NA
15 hsa-miR-29b-3p AMMECR1L 0.18 0.08561 -0.09 0.025 MirTarget; miRNATAP -0.17 0 NA
16 hsa-miR-29b-3p ANKRD13B 0.18 0.08561 0.09 0.14467 MirTarget; miRNATAP -0.2 0 NA
17 hsa-miR-29b-3p ARHGAP19 0.18 0.08561 -0.03 0.4825 mirMAP -0.15 0 NA
18 hsa-miR-29b-3p ARID3A 0.18 0.08561 -0.24 6.0E-5 mirMAP -0.13 0 NA
19 hsa-miR-29b-3p ARRDC3 0.18 0.08561 -0.12 0.04815 MirTarget; miRNATAP -0.1 4.0E-5 NA
20 hsa-miR-29b-3p ARVCF 0.18 0.08561 0.13 0.01897 MirTarget; miRNATAP -0.16 0 NA
21 hsa-miR-29b-3p ATAD2B 0.18 0.08561 -0.07 0.17656 MirTarget; miRNATAP -0.24 0 NA
22 hsa-miR-29b-3p ATP7A 0.18 0.08561 -0.18 0.00025 miRNATAP -0.12 0 NA
23 hsa-miR-29b-3p BACH2 0.18 0.08561 -0.06 0.32181 MirTarget; miRNATAP -0.2 0 NA
24 hsa-miR-29b-3p BIRC6 0.18 0.08561 0.12 0.05351 miRNATAP -0.13 0 NA
25 hsa-miR-29b-3p BLMH 0.18 0.08561 -0.12 0.01017 MirTarget; miRNATAP -0.17 0 NA
26 hsa-miR-29b-3p BMF 0.18 0.08561 -0.43 1.0E-5 MirTarget; miRNATAP -0.26 0 NA
27 hsa-miR-29b-3p BMP1 0.18 0.08561 -0.3 0 miRNAWalker2 validate; miRTarBase; miRNATAP -0.22 0 NA
28 hsa-miR-29b-3p BRD3 0.18 0.08561 0.08 0.14277 miRNATAP -0.21 0 NA
29 hsa-miR-29b-3p BRWD3 0.18 0.08561 -0.07 0.49067 MirTarget; miRNATAP -0.36 0 NA
30 hsa-miR-29b-3p BTBD7 0.18 0.08561 0.09 0.07012 miRNATAP -0.12 0 NA
31 hsa-miR-29b-3p C16orf87 0.18 0.08561 -0.11 0.02794 mirMAP; miRNATAP -0.19 0 NA
32 hsa-miR-29b-3p C1QTNF6 0.18 0.08561 -0.32 1.0E-5 miRNATAP -0.26 0 NA
33 hsa-miR-29b-3p CACNG4 0.18 0.08561 0.06 0.65731 miRNATAP -0.5 0 NA
34 hsa-miR-29b-3p CASZ1 0.18 0.08561 -0.06 0.59914 miRNATAP -0.2 0.00025 NA
35 hsa-miR-29b-3p CBX2 0.18 0.08561 -0.58 0 miRNATAP -0.43 0 NA
36 hsa-miR-29b-3p CBX5 0.18 0.08561 -0.06 0.31998 miRNATAP -0.15 0 NA
37 hsa-miR-29b-3p CBX8 0.18 0.08561 -0.24 0 mirMAP -0.15 0 NA
38 hsa-miR-29b-3p CCDC117 0.18 0.08561 0.03 0.51322 MirTarget; miRNATAP -0.11 0 NA
39 hsa-miR-29b-3p CCNA2 0.18 0.08561 -1.13 0 MirTarget -0.4 0 NA
40 hsa-miR-29b-3p CCND1 0.18 0.08561 -0.52 0 mirMAP -0.31 0 NA
41 hsa-miR-29b-3p CCND2 0.18 0.08561 -0.28 0.00231 MirTarget; miRNATAP -0.2 0 22330340; 24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC
42 hsa-miR-29b-3p CCNJ 0.18 0.08561 -0.04 0.44486 MirTarget -0.24 0 NA
43 hsa-miR-29b-3p CD276 0.18 0.08561 -0.46 0 MirTarget; miRNATAP -0.18 0 NA
44 hsa-miR-29b-3p CD93 0.18 0.08561 -0.97 0 MirTarget -0.25 2.0E-5 NA
45 hsa-miR-29b-3p CDC42SE1 0.18 0.08561 -0.09 0.05934 MirTarget -0.13 0 NA
46 hsa-miR-29b-3p CDCA4 0.18 0.08561 -0.52 0 MirTarget -0.26 0 NA
47 hsa-miR-29b-3p CDK6 0.18 0.08561 -0.51 1.0E-5 miRNAWalker2 validate; miRTarBase; miRNATAP -0.27 0 26180082; 23245396; 25472644; 23591808; 27230400; 20086245 Knockdown of NTSR1 increased the expression of miR-29b-1 and miR-129-3p which were responsible for the decreased CDK6 expression;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;Moreover miR-29b inhibited the expression of MCL1 and CDK6;Here we have identified the oncogene cyclin-dependent protein kinase 6 CDK6 as a direct target of miR-29b in lung cancer;MiR 29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6; In this study we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods; We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells; Furthermore we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues; The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL
48 hsa-miR-29b-3p CECR2 0.18 0.08561 0.21 0.02445 miRNAWalker2 validate -0.36 0 NA
49 hsa-miR-29b-3p CLCN5 0.18 0.08561 -0.11 0.02027 miRNATAP -0.14 0 NA
50 hsa-miR-29b-3p CLK2 0.18 0.08561 -0.03 0.56085 MirTarget; miRNATAP -0.12 0 NA
51 hsa-miR-29b-3p COL1A1 0.18 0.08561 -1.24 0 miRNAWalker2 validate; miRTarBase; miRNATAP -0.24 0.01364 NA
52 hsa-miR-29b-3p COL1A2 0.18 0.08561 -0.95 0 miRNATAP -0.13 0.03544 NA
53 hsa-miR-29b-3p COL2A1 0.18 0.08561 -0.75 0.00037 MirTarget; miRNATAP -0.32 0.00134 NA
54 hsa-miR-29b-3p COL3A1 0.18 0.08561 -1.43 0 miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP -0.44 0 NA
55 hsa-miR-29b-3p COL4A1 0.18 0.08561 -1.37 0 miRNAWalker2 validate; miRTarBase; miRNATAP -0.35 0 NA
56 hsa-miR-29b-3p COL4A2 0.18 0.08561 -1.21 0 miRTarBase; MirTarget; miRNATAP -0.24 0.0002 NA
57 hsa-miR-29b-3p COL4A4 0.18 0.08561 0.01 0.93704 MirTarget; miRNATAP -0.23 4.0E-5 NA
58 hsa-miR-29b-3p COL7A1 0.18 0.08561 0.01 0.93339 MirTarget; miRNATAP -0.26 7.0E-5 NA
59 hsa-miR-29b-3p COL9A1 0.18 0.08561 -0.28 0.17201 miRNATAP -0.23 0.01927 NA
60 hsa-miR-29b-3p COMMD2 0.18 0.08561 -0.09 0.06189 MirTarget; miRNATAP -0.14 0 NA
61 hsa-miR-29b-3p COQ5 0.18 0.08561 -0.03 0.40527 MirTarget -0.1 0 NA
62 hsa-miR-29b-3p CRISPLD1 0.18 0.08561 -0.54 0 MirTarget; miRNATAP -0.23 0 NA
63 hsa-miR-29b-3p CTDSPL2 0.18 0.08561 -0.09 0.09901 MirTarget -0.15 0 NA
64 hsa-miR-29b-3p CTNND1 0.18 0.08561 -0.14 0.01752 miRNATAP -0.13 0 NA
65 hsa-miR-29b-3p DCAF12 0.18 0.08561 -0.05 0.33237 miRNATAP -0.18 0 NA
66 hsa-miR-29b-3p DCAF5 0.18 0.08561 0.16 0.00087 miRNATAP -0.1 0 NA
67 hsa-miR-29b-3p DCAF7 0.18 0.08561 -0 0.95508 MirTarget; miRNATAP -0.2 0 NA
68 hsa-miR-29b-3p DCX 0.18 0.08561 0.35 0.02122 MirTarget; miRNATAP -0.59 0 NA
69 hsa-miR-29b-3p DDX46 0.18 0.08561 -0.03 0.48875 MirTarget -0.12 0 NA
70 hsa-miR-29b-3p DENND1B 0.18 0.08561 -0.03 0.68777 miRNATAP -0.14 1.0E-5 NA
71 hsa-miR-29b-3p DGCR2 0.18 0.08561 0.25 0.00027 mirMAP -0.15 0 NA
72 hsa-miR-29b-3p DGKD 0.18 0.08561 0.06 0.29383 MirTarget; miRNATAP -0.15 0 NA
73 hsa-miR-29b-3p DLGAP1 0.18 0.08561 0.62 0 miRNATAP -0.13 0.02142 NA
74 hsa-miR-29b-3p DNMT1 0.18 0.08561 -0.33 0 miRNAWalker2 validate; miRTarBase -0.2 0 21625215 MiRNA-dependent regulation of MEG3 expression was studied by evaluating the involvement of miR-29 which can modulate DNMT 1 and 3
75 hsa-miR-29b-3p DNMT3A 0.18 0.08561 -0.27 0 miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP -0.12 0 23939044; 26171207; 24798303; 26471361; 25874772; 17890317; 22479643; 25746661 The miR-29 family miR-29a -29b and -29c which negatively regulates DNMT3A and DNMT3B was examined in association with the Wnt/β-catenin signaling pathway; The expression of miR-29a and miR-29b was partially regulated by DNMT3A and DNMT3B in a positive feedback loop;In addition a significant inverse correlation was identified between the expression levels of DNMT3A and miR-29b in estrogen receptor-positive breast cancer patients P=0.027;Consistent with miR-29's role in targeting DNA methyltransferase 3A DNMT3A a key enzyme regulating DNA methylation we found a significant inverse correlation P<0.001 between miR-29 and DNMT3A gene expression suggesting that they might be functionally antagonistic;Mechanistically ATDC exerted its oncogenic effects by suppressing miR-29 and subsequent upregulation of DNMT3A leading to DNA methylation and silencing of the tumor suppressor PTEN;The de-regulation of the miR-29 family and DNA methyltransferase 3A DNMT3A is associated with gastric cancer GC;Among the reported down-regulated miRNAs in lung cancer the miRNA miR-29 family 29a 29b and 29c has intriguing complementarities to the 3'-UTRs of DNA methyltransferase DNMT3A and -3B de novo methyltransferases two key enzymes involved in DNA methylation that are frequently up-regulated in lung cancer and associated with poor prognosis;The miR-29 family and predicted target genes were among the most strongly anti-correlated miRNA:mRNA pairs; over-expression of miR-29a in vitro repressed several anti-correlated genes including DNMT3A and DNMT3B and substantially decreased ovarian cancer cell viability;DNMT3A and 3B genes can be regulated post-transcriptionally by miR-29 family
76 hsa-miR-29b-3p DNMT3B 0.18 0.08561 -0.33 0 miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP -0.26 0 23939044; 26404322; 22479643 The miR-29 family miR-29a -29b and -29c which negatively regulates DNMT3A and DNMT3B was examined in association with the Wnt/β-catenin signaling pathway; The expression of miR-29a and miR-29b was partially regulated by DNMT3A and DNMT3B in a positive feedback loop;The induction of miR-29b was accompanied with suppression of its downstream target DNMT3B in a dose-dependent manner; The reduction of luciferase activity of DNMT3B 3'-UTR reporter by fucoidan was as markedly as that by miR-29b mimic indicating that fucoidan induced miR-29b to suppress DNMT3B;The miR-29 family and predicted target genes were among the most strongly anti-correlated miRNA:mRNA pairs; over-expression of miR-29a in vitro repressed several anti-correlated genes including DNMT3A and DNMT3B and substantially decreased ovarian cancer cell viability
77 hsa-miR-29b-3p DOLPP1 0.18 0.08561 -0.05 0.19957 MirTarget; miRNATAP -0.11 0 NA
78 hsa-miR-29b-3p DOT1L 0.18 0.08561 -0.2 0.00987 MirTarget; miRNATAP -0.36 0 NA
79 hsa-miR-29b-3p DPYSL3 0.18 0.08561 0.1 0.2298 MirTarget -0.26 0 NA
80 hsa-miR-29b-3p DTX4 0.18 0.08561 0.32 0.00014 miRNATAP -0.1 0.00401 NA
81 hsa-miR-29b-3p DYNLT1 0.18 0.08561 -0.21 0.00063 miRNATAP -0.22 0 NA
82 hsa-miR-29b-3p E2F7 0.18 0.08561 -1.47 0 miRNATAP -0.39 0 22330340 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator
83 hsa-miR-29b-3p EDC3 0.18 0.08561 0.03 0.52019 MirTarget; miRNATAP -0.15 0 NA
84 hsa-miR-29b-3p ELF2 0.18 0.08561 -0.07 0.09471 MirTarget; miRNATAP -0.12 0 NA
85 hsa-miR-29b-3p ELN 0.18 0.08561 -0.53 0 MirTarget; miRNATAP -0.2 0 NA
86 hsa-miR-29b-3p EML4 0.18 0.08561 -0.29 0 MirTarget; miRNATAP -0.15 0 NA
87 hsa-miR-29b-3p EPHB3 0.18 0.08561 -0.19 0.01544 miRNATAP -0.29 0 NA
88 hsa-miR-29b-3p FLVCR2 0.18 0.08561 0.11 0.27506 MirTarget -0.25 0 NA
89 hsa-miR-29b-3p FYN 0.18 0.08561 0.05 0.37684 miRNATAP -0.15 0 NA
90 hsa-miR-29b-3p GAB1 0.18 0.08561 -0.04 0.47459 MirTarget -0.13 0 NA
91 hsa-miR-29b-3p GLIS2 0.18 0.08561 0.02 0.73656 MirTarget; miRNATAP -0.27 0 NA
92 hsa-miR-29b-3p GNA13 0.18 0.08561 -0.02 0.78967 MirTarget -0.11 0 NA
93 hsa-miR-29b-3p GNB4 0.18 0.08561 -0.15 0.04001 MirTarget -0.19 0 NA
94 hsa-miR-29b-3p GPATCH2 0.18 0.08561 -0.04 0.25585 MirTarget -0.13 0 NA
95 hsa-miR-29b-3p GPR173 0.18 0.08561 0.26 0.00062 mirMAP -0.25 0 NA
96 hsa-miR-29b-3p GRIK3 0.18 0.08561 -0.13 0.21403 mirMAP -0.29 0 NA
97 hsa-miR-29b-3p GSK3B 0.18 0.08561 0.01 0.89186 miRTarBase; miRNATAP -0.1 0 NA
98 hsa-miR-29b-3p GSTA4 0.18 0.08561 0.17 0.00165 miRNATAP -0.15 0 NA
99 hsa-miR-29b-3p GTPBP1 0.18 0.08561 0.08 0.13085 mirMAP -0.11 0 NA
100 hsa-miR-29b-3p HAPLN3 0.18 0.08561 -0.59 0 MirTarget -0.17 0.00033 NA
101 hsa-miR-29b-3p HAS3 0.18 0.08561 -0.25 0.0003 miRNATAP -0.23 0 NA
102 hsa-miR-29b-3p HDAC4 0.18 0.08561 0.39 0 miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP -0.18 0 NA
103 hsa-miR-29b-3p HELZ 0.18 0.08561 -0.02 0.7556 MirTarget -0.13 0 NA
104 hsa-miR-29b-3p HMCN1 0.18 0.08561 -0.23 0.02301 MirTarget; miRNATAP -0.3 0 NA
105 hsa-miR-29b-3p HMGCR 0.18 0.08561 0.23 0.00294 miRNATAP -0.11 0.00128 NA
106 hsa-miR-29b-3p HNF4G 0.18 0.08561 -0.28 0.00371 MirTarget; miRNATAP -0.16 0.00074 NA
107 hsa-miR-29b-3p HNRNPF 0.18 0.08561 -0.15 0.00366 miRNATAP -0.1 0 NA
108 hsa-miR-29b-3p HNRNPUL1 0.18 0.08561 -0.11 0.07847 miRNATAP -0.11 0 NA
109 hsa-miR-29b-3p ID1 0.18 0.08561 0 0.9757 MirTarget -0.25 0 22249264; 24662327 Incubation of lung cancer cells with the Src inhibitor saracatinib led to the upregulation of several miRNAs including miR-29b which was the most highly upregulated miRNA with predicted binding to the ID1 3'-untranslated region UTR; Luciferase reporter assays confirmed direct binding of miR-29b to the ID1 3'-UTR; Expression of miR-29b suppressed ID1 levels and significantly reduced migration and invasion;Id 1 a protein repressed by miR 29b facilitates the TGFβ1 induced epithelial mesenchymal transition in human ovarian cancer cells
110 hsa-miR-29b-3p IGF1R 0.18 0.08561 0.14 0.0366 mirMAP -0.11 1.0E-5 NA
111 hsa-miR-29b-3p ILDR2 0.18 0.08561 -0.21 0.12015 MirTarget -0.28 1.0E-5 NA
112 hsa-miR-29b-3p ISG20L2 0.18 0.08561 -0.15 0.00047 MirTarget -0.14 0 NA
113 hsa-miR-29b-3p JARID2 0.18 0.08561 0.14 0.00609 MirTarget; miRNATAP -0.12 0 NA
114 hsa-miR-29b-3p KCTD15 0.18 0.08561 0.02 0.74648 MirTarget; miRNATAP -0.15 0 NA
115 hsa-miR-29b-3p KCTD20 0.18 0.08561 0.05 0.49428 MirTarget -0.17 0 NA
116 hsa-miR-29b-3p KCTD3 0.18 0.08561 0.02 0.74148 miRNATAP -0.15 0 NA
117 hsa-miR-29b-3p KCTD5 0.18 0.08561 -0.07 0.12512 MirTarget; miRNATAP -0.19 0 NA
118 hsa-miR-29b-3p KDELC1 0.18 0.08561 -0.31 0 miRNATAP -0.28 0 NA
119 hsa-miR-29b-3p KDM5A 0.18 0.08561 -0.13 0.02989 miRNATAP -0.16 0 NA
120 hsa-miR-29b-3p KDM5B 0.18 0.08561 0 0.96593 MirTarget -0.23 0 NA
121 hsa-miR-29b-3p KDM6B 0.18 0.08561 -0.02 0.72325 miRNATAP -0.25 0 NA
122 hsa-miR-29b-3p KIAA0895L 0.18 0.08561 0.21 0.00974 MirTarget; miRNATAP -0.19 0 NA
123 hsa-miR-29b-3p KIAA1549 0.18 0.08561 0.18 0.01821 miRNATAP -0.27 0 NA
124 hsa-miR-29b-3p KIF21B 0.18 0.08561 0.57 0 mirMAP -0.14 0.00061 NA
125 hsa-miR-29b-3p KIF26A 0.18 0.08561 0.2 0.12211 miRNATAP -0.54 0 NA
126 hsa-miR-29b-3p KIF26B 0.18 0.08561 -0.36 0.00285 miRNATAP -0.14 0.01379 NA
127 hsa-miR-29b-3p KLHL25 0.18 0.08561 0.42 0 MirTarget -0.3 0 NA
128 hsa-miR-29b-3p LAMC1 0.18 0.08561 -0.64 0 MirTarget; miRNATAP -0.15 0.00022 NA
129 hsa-miR-29b-3p LDOC1L 0.18 0.08561 0.18 0.00268 MirTarget; miRNATAP -0.14 0 NA
130 hsa-miR-29b-3p LIF 0.18 0.08561 -1.17 0 MirTarget; miRNATAP -0.17 0.03493 NA
131 hsa-miR-29b-3p LIMS1 0.18 0.08561 -0.2 0.02024 MirTarget; miRNATAP -0.16 3.0E-5 NA
132 hsa-miR-29b-3p LMX1A 0.18 0.08561 0.85 0.00067 miRNATAP -0.41 0.00054 NA
133 hsa-miR-29b-3p LPL 0.18 0.08561 0.69 0 miRNATAP -0.2 0.00214 NA
134 hsa-miR-29b-3p LRCH3 0.18 0.08561 -0.08 0.21559 mirMAP -0.22 0 NA
135 hsa-miR-29b-3p LRP6 0.18 0.08561 0.06 0.30332 miRNATAP -0.15 0 NA
136 hsa-miR-29b-3p MAFB 0.18 0.08561 0.11 0.20024 miRNATAP -0.1 0.01008 NA
137 hsa-miR-29b-3p MAPRE1 0.18 0.08561 -0.06 0.23602 MirTarget -0.17 0 NA
138 hsa-miR-29b-3p MARCH1 0.18 0.08561 0.1 0.22337 miRNATAP -0.13 0.0011 NA
139 hsa-miR-29b-3p MAZ 0.18 0.08561 -0.04 0.54539 miRNATAP -0.13 0 NA
140 hsa-miR-29b-3p MCL1 0.18 0.08561 -0.14 0.04605 miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP -0.1 2.0E-5 24155920; 20041405; 25472644; 27063186; 19850741; 24992675 Finally miR-29a and miR-29b targeted MCL1 mRNA in GICs and increased apoptosis;Subsequent investigation characterized two antiapoptotic molecules Bcl-2 and Mcl-1 as direct targets of miR-29; Furthermore silencing of Bcl-2 and Mcl-1 phenocopied the proapoptotic effect of miR-29 whereas overexpression of these proteins attenuated the effect of miR-29;Moreover miR-29b inhibited the expression of MCL1 and CDK6;miR 29b and miR 198 overexpression in CD8+ T cells of renal cell carcinoma patients down modulates JAK3 and MCL 1 leading to immune dysfunction; In particular JAK3 and MCL-1 were down-regulated in patient CD8+ T cells versus their normal counterparts likely due to defective suppressor activity of miR-29b and miR-198 in RCC CD8+ T cells; Indeed specific inhibition of miR-29b or miR-198 in peripheral blood mononuclear cells PBMCs isolated from RCC patients resulted in the up-regulation of JAK3 and MCL-1 proteins and significant improvement of cell survival in vitro;A significant enrichment for apoptosis genes including MCL-1 was found among the mRNAs inversely correlated with miR-29b expression in 45 primary AML samples;BAG3 upregulates Mcl 1 through downregulation of miR 29b to induce anticancer drug resistance in ovarian cancer; Upregulation of miR-29b also reduced levels of Mcl-1 and sensitized ES2 cells to low-dose paclitaxel; BAG3 knockdown appears to downregulate expression of Mcl-1 through upregulation of miR-29b thereby increasing the chemosensitivity of ovarian clear cell carcinoma cells
141 hsa-miR-29b-3p MED26 0.18 0.08561 -0.08 0.01725 miRNATAP -0.1 0 NA
142 hsa-miR-29b-3p MEST 0.18 0.08561 -0.58 0 MirTarget; miRNATAP -0.31 0 NA
143 hsa-miR-29b-3p MEX3B 0.18 0.08561 0.14 0.15689 miRNATAP -0.39 0 NA
144 hsa-miR-29b-3p MFAP2 0.18 0.08561 -1.34 0 miRNATAP -0.21 0.02756 NA
145 hsa-miR-29b-3p MIER3 0.18 0.08561 -0.06 0.19132 MirTarget; miRNATAP -0.14 0 NA
146 hsa-miR-29b-3p MKI67 0.18 0.08561 -1.51 0 miRNAWalker2 validate -0.74 0 NA
147 hsa-miR-29b-3p MLXIP 0.18 0.08561 -0.19 0.00037 MirTarget -0.13 0 NA
148 hsa-miR-29b-3p MMD2 0.18 0.08561 0.72 0 miRNATAP -0.15 0.02776 NA
149 hsa-miR-29b-3p MMP15 0.18 0.08561 -0.1 0.23201 miRNAWalker2 validate -0.3 0 NA
150 hsa-miR-29b-3p MMP2 0.18 0.08561 -0.7 0 miRNAWalker2 validate; miRTarBase; miRNATAP -0.28 0 26155940; 20657750; 25866219; 24130681; 24130168; 26063204 In this study we found that only miR-29b inhibited tumor cell migration and invasion by reducing MMP-2 activity via phospho-AKT/β-catenin signaling and stimulated a more epithelial-like morphology;Further a novel target with potential implications for invasion and metastasis matrix metallopeptidase-2 MMP-2 was identified and confirmed to be a miR-29b target in prostate cancer cells;miR 29b inhibits the progression of esophageal squamous cell carcinoma by targeting MMP 2; The impact of overexpression of miR-29b on putative target MMP-2 were subsequently confirmed via Western blot; Overexpression of miR-29b significantly decreased p<0.05 the protein level of MMP-2 which has previously been identified as a direct target of miR-29b; Thus our study demonstrated that overexpression of miR-29b inhibits tumor growth in part by targeting MMP-2;Since miR-29b plays a role in regulating the migration of cancer cells we hypothesized that HAG induces miR-29b expression to target matrix metalloproteinase-2 MMP-2 thereby suppressing the migration of colon cancer cells; Our study supports the understanding that targeting MMP-2 by miR-29b is a mechanism by which HAG suppresses the migration of colon cancer cells;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC;Furthermore the dual-luciferase reporter assay demonstrated that miR-29b inhibited the expression of the luciferase gene containing the 3'-UTRs of MMP2 and PTEN mRNA; Western blotting and quantitative RT-PCR indicated that miR-29b down-regulated the expression of MMP2 at the protein and mRNA levels; Taken together our results demonstrate that miR-29b serves as a tumor metastasis suppressor which suppresses NSCLC cell metastasis by directly inhibiting MMP2 expression

Over-represented Gene Ontology Biological Process

NumGOOverlapSizeP ValueAdj. P Value
1 REGULATION OF TRANSCRIPTION FROM RNA POLYMERASE II PROMOTER 60 1784 9.909e-11 4.611e-07
2 EXTRACELLULAR STRUCTURE ORGANIZATION 22 304 3.387e-10 7.88e-07
3 MULTICELLULAR ORGANISMAL MACROMOLECULE METABOLIC PROCESS 12 79 9.569e-10 1.484e-06
4 MULTICELLULAR ORGANISM METABOLIC PROCESS 12 93 6.545e-09 7.614e-06
5 REGULATION OF MULTICELLULAR ORGANISMAL DEVELOPMENT 52 1672 3.18e-08 2.96e-05
6 CHROMATIN MODIFICATION 26 539 4.359e-08 3.381e-05
7 CENTRAL NERVOUS SYSTEM DEVELOPMENT 33 872 2.036e-07 0.0001353
8 POSITIVE REGULATION OF GENE EXPRESSION 51 1733 2.508e-07 0.0001459
9 REGULATION OF CELL DIFFERENTIATION 46 1492 2.938e-07 0.0001519
10 TISSUE DEVELOPMENT 46 1518 4.793e-07 0.000223
11 CHROMATIN ORGANIZATION 27 663 6.996e-07 0.0002504
12 CARDIOVASCULAR SYSTEM DEVELOPMENT 30 788 6.718e-07 0.0002504
13 CIRCULATORY SYSTEM DEVELOPMENT 30 788 6.718e-07 0.0002504
14 VASCULATURE DEVELOPMENT 22 469 7.943e-07 0.000264
15 CELLULAR RESPONSE TO OXYGEN CONTAINING COMPOUND 30 799 8.952e-07 0.0002777
16 POSITIVE REGULATION OF BIOSYNTHETIC PROCESS 50 1805 1.993e-06 0.0005796
17 NEUROGENESIS 42 1402 2.181e-06 0.0005822
18 REGULATION OF CELL DEVELOPMENT 30 836 2.252e-06 0.0005822
19 ORGAN MORPHOGENESIS 30 841 2.539e-06 0.0006218
20 NEGATIVE REGULATION OF CHROMATIN MODIFICATION 7 45 2.75e-06 0.0006398
21 REGULATION OF GENE EXPRESSION EPIGENETIC 14 229 4.39e-06 0.0009728
22 CELLULAR RESPONSE TO ENDOGENOUS STIMULUS 33 1008 4.896e-06 0.001036
23 CARTILAGE DEVELOPMENT 11 147 7.074e-06 0.001407
24 REGULATION OF CHROMATIN SILENCING 5 21 8.637e-06 0.001407
25 RESPONSE TO OXYGEN CONTAINING COMPOUND 40 1381 8.772e-06 0.001407
26 HEAD DEVELOPMENT 26 709 7.581e-06 0.001407
27 CELLULAR RESPONSE TO AMINO ACID STIMULUS 7 53 8.493e-06 0.001407
28 NEGATIVE REGULATION OF DEVELOPMENTAL PROCESS 28 801 8.162e-06 0.001407
29 REGULATION OF GENE SILENCING 7 52 7.459e-06 0.001407
30 REGULATION OF CELL PROLIFERATION 42 1496 1.087e-05 0.001685
31 ANATOMICAL STRUCTURE FORMATION INVOLVED IN MORPHOGENESIS 31 957 1.194e-05 0.001736
32 POSITIVE REGULATION OF TRANSCRIPTION FROM RNA POLYMERASE II PROMOTER 32 1004 1.184e-05 0.001736
33 RESPONSE TO GROWTH FACTOR 20 475 1.265e-05 0.001784
34 COLLAGEN FIBRIL ORGANIZATION 6 38 1.325e-05 0.001813
35 RESPONSE TO ALCOHOL 17 362 1.441e-05 0.001915
36 BLOOD VESSEL MORPHOGENESIS 17 364 1.547e-05 0.001945
37 CELLULAR RESPONSE TO REACTIVE OXYGEN SPECIES 9 104 1.52e-05 0.001945
38 ANGIOGENESIS 15 293 1.699e-05 0.002081
39 CONNECTIVE TISSUE DEVELOPMENT 12 194 1.893e-05 0.002259
40 PEPTIDYL AMINO ACID MODIFICATION 28 841 1.985e-05 0.002309
41 EMBRYO DEVELOPMENT 29 894 2.264e-05 0.002569
42 SKELETAL SYSTEM DEVELOPMENT 19 455 2.343e-05 0.002596
43 POSITIVE REGULATION OF CHROMATIN MODIFICATION 8 85 2.466e-05 0.002669
44 POSITIVE REGULATION OF DEVELOPMENTAL PROCESS 34 1142 2.55e-05 0.002696
45 POSITIVE REGULATION OF NEURON DIFFERENTIATION 15 306 2.818e-05 0.002913
46 COVALENT CHROMATIN MODIFICATION 16 345 2.999e-05 0.003033
47 CHROMOSOME ORGANIZATION 31 1009 3.305e-05 0.003272
48 PEPTIDYL LYSINE MODIFICATION 15 312 3.524e-05 0.003352
49 RESPONSE TO TRANSFORMING GROWTH FACTOR BETA 10 144 3.53e-05 0.003352
50 CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION 20 513 3.784e-05 0.003522
51 RESPONSE TO ESTRADIOL 10 146 3.973e-05 0.003555
52 NEURON DIFFERENTIATION 28 874 3.931e-05 0.003555
53 GLAND DEVELOPMENT 17 395 4.349e-05 0.003818
54 NEGATIVE REGULATION OF GENE EXPRESSION 40 1493 5.177e-05 0.00438
55 REGULATION OF EPITHELIAL CELL PROLIFERATION 14 285 5.088e-05 0.00438
56 NEURON PROJECTION MORPHOGENESIS 17 402 5.407e-05 0.004492
57 REGULATION OF CHROMATIN ORGANIZATION 10 152 5.594e-05 0.004567
58 RESPONSE TO ENDOGENOUS STIMULUS 39 1450 5.97e-05 0.004789
59 CELL MORPHOGENESIS INVOLVED IN NEURON DIFFERENTIATION 16 368 6.472e-05 0.005104
60 NEGATIVE REGULATION OF NITROGEN COMPOUND METABOLIC PROCESS 40 1517 7.336e-05 0.005689
61 REGULATION OF HISTONE H3 K9 METHYLATION 4 17 7.644e-05 0.005831
62 POSITIVE REGULATION OF CELL PROLIFERATION 26 814 7.874e-05 0.00591
63 PROTEIN AUTOPHOSPHORYLATION 11 192 8.427e-05 0.006224
64 POSITIVE REGULATION OF HISTONE METHYLATION 5 33 8.784e-05 0.006386
65 CELLULAR RESPONSE TO NITROGEN COMPOUND 19 505 9.463e-05 0.006774
66 PROTEIN MODIFICATION BY SMALL PROTEIN CONJUGATION OR REMOVAL 27 873 9.785e-05 0.006898
67 NEURON DEVELOPMENT 23 687 0.0001034 0.007075
68 DEMETHYLATION 6 54 0.0001028 0.007075
69 REGULATION OF NEURON DIFFERENTIATION 20 554 0.0001086 0.007325
70 POSITIVE REGULATION OF CELL DEVELOPMENT 18 472 0.0001222 0.007786
71 NEGATIVE REGULATION OF MULTICELLULAR ORGANISMAL PROCESS 29 983 0.0001219 0.007786
72 NEGATIVE REGULATION OF TRANSCRIPTION FROM RNA POLYMERASE II PROMOTER 24 740 0.0001186 0.007786
73 BIOLOGICAL ADHESION 30 1032 0.0001202 0.007786
74 RESPONSE TO ETHANOL 9 136 0.0001257 0.007903
75 NEGATIVE REGULATION OF HISTONE MODIFICATION 5 36 0.0001348 0.008366
76 NEGATIVE REGULATION OF CELL DIFFERENTIATION 21 609 0.0001385 0.00848
77 REGULATION OF NERVOUS SYSTEM DEVELOPMENT 24 750 0.0001452 0.008773
78 POSITIVE REGULATION OF NERVOUS SYSTEM DEVELOPMENT 17 437 0.0001487 0.008869
79 NEURON PROJECTION GUIDANCE 11 205 0.0001506 0.008869
80 DNA METHYLATION OR DEMETHYLATION 6 59 0.0001693 0.009845
NumGOOverlapSizeP ValueAdj. P Value
1 EXTRACELLULAR MATRIX STRUCTURAL CONSTITUENT 12 76 6.016e-10 5.589e-07
2 PLATELET DERIVED GROWTH FACTOR BINDING 6 11 3.043e-09 1.414e-06
3 MACROMOLECULAR COMPLEX BINDING 47 1399 1.624e-08 5.029e-06
4 REGULATORY REGION NUCLEIC ACID BINDING 31 818 4.763e-07 0.0001106
5 TRANSCRIPTION FACTOR ACTIVITY PROTEIN BINDING 25 588 8.484e-07 0.0001576
6 ZINC ION BINDING 37 1155 2.051e-06 0.0003176
7 METALLOENDOPEPTIDASE ACTIVITY 10 113 4.17e-06 0.0005534
8 TRANSITION METAL ION BINDING 40 1400 1.207e-05 0.001298
9 CHROMATIN BINDING 19 435 1.257e-05 0.001298
10 PROTEIN COMPLEX BINDING 30 935 1.995e-05 0.001853
11 SEQUENCE SPECIFIC DNA BINDING 32 1037 2.246e-05 0.001897
12 TRANSCRIPTION FACTOR BINDING 20 524 5.083e-05 0.003935
13 NUCLEIC ACID BINDING TRANSCRIPTION FACTOR ACTIVITY 34 1199 6.728e-05 0.004808
14 DOUBLE STRANDED DNA BINDING 25 764 7.413e-05 0.004919
15 HISTONE DEACETYLASE BINDING 8 105 0.0001124 0.006527
16 TRANSCRIPTION FACTOR ACTIVITY RNA POLYMERASE II TRANSCRIPTION FACTOR BINDING 9 133 0.000106 0.006527
NumGOOverlapSizeP ValueAdj. P Value
1 PROTEINACEOUS EXTRACELLULAR MATRIX 28 356 1.521e-13 8.883e-11
2 EXTRACELLULAR MATRIX 30 426 3.522e-13 1.028e-10
3 EXTRACELLULAR MATRIX COMPONENT 17 125 1.827e-12 3.556e-10
4 COMPLEX OF COLLAGEN TRIMERS 8 23 5.306e-10 7.747e-08
5 COLLAGEN TRIMER 10 88 4.116e-07 4.807e-05
6 BASEMENT MEMBRANE 10 93 6.93e-07 6.745e-05
7 CHROMATIN 20 441 4.241e-06 0.0003539
8 BANDED COLLAGEN FIBRIL 4 12 1.68e-05 0.001226
9 PCG PROTEIN COMPLEX 6 43 2.76e-05 0.001612
10 ENDOPLASMIC RETICULUM LUMEN 12 201 2.691e-05 0.001612
11 CHROMOSOME 27 880 0.0001116 0.005925

Over-represented Pathway

NumPathwayPathviewOverlapSizeP ValueAdj. P Value
1 Focal_adhesion_hsa04510 16 199 2.123e-08 1.104e-06
2 PI3K_Akt_signaling_pathway_hsa04151 18 352 2.475e-06 6.435e-05
3 ECM_receptor_interaction_hsa04512 8 82 1.893e-05 0.0003282
4 Cell_cycle_hsa04110 8 124 0.0003536 0.004597
5 Signaling_pathways_regulating_pluripotency_of_stem_cells_hsa04550 8 139 0.0007561 0.007863
6 ErbB_signaling_pathway_hsa04012 5 85 0.006748 0.04739
7 AMPK_signaling_pathway_hsa04152 6 121 0.007058 0.04739
8 Cellular_senescence_hsa04218 7 160 0.007291 0.04739
9 p53_signaling_pathway_hsa04115 4 68 0.01502 0.07913
10 Ras_signaling_pathway_hsa04014 8 232 0.01649 0.07913
11 Phospholipase_D_signaling_pathway_hsa04072 6 146 0.01674 0.07913
12 Jak_STAT_signaling_pathway_hsa04630 6 162 0.02633 0.1088
13 Hedgehog_signaling_pathway_hsa04340 3 47 0.02774 0.1088
14 Notch_signaling_pathway_hsa04330 3 48 0.02929 0.1088
15 FoxO_signaling_pathway_hsa04068 5 132 0.03779 0.131
16 Phosphatidylinositol_signaling_system_hsa04070 4 99 0.04991 0.1622
17 Hippo_signaling_pathway_hsa04390 5 154 0.06494 0.1986
18 Rap1_signaling_pathway_hsa04015 6 206 0.06933 0.2003
19 Adherens_junction_hsa04520 3 72 0.07948 0.2175
20 Apelin_signaling_pathway_hsa04371 4 137 0.1252 0.3256
21 Wnt_signaling_pathway_hsa04310 4 146 0.1476 0.3656
22 mTOR_signaling_pathway_hsa04150 4 151 0.1607 0.3705
23 HIF_1_signaling_pathway_hsa04066 3 100 0.1639 0.3705
24 VEGF_signaling_pathway_hsa04370 2 59 0.199 0.4311
25 Sphingolipid_signaling_pathway_hsa04071 3 118 0.2277 0.4736
26 Autophagy_animal_hsa04140 3 128 0.2649 0.5298
27 TGF_beta_signaling_pathway_hsa04350 2 84 0.3277 0.6004
28 Regulation_of_actin_cytoskeleton_hsa04810 4 208 0.3304 0.6004
29 TNF_signaling_pathway_hsa04668 2 108 0.446 0.7482
30 cAMP_signaling_pathway_hsa04024 3 198 0.5237 0.8252
31 Apoptosis_hsa04210 2 138 0.5761 0.8475
32 MAPK_signaling_pathway_hsa04010 4 295 0.5918 0.8475
33 Cell_adhesion_molecules_.CAMs._hsa04514 2 145 0.603 0.8475
34 Endocytosis_hsa04144 3 244 0.6645 0.8885
35 cGMP_PKG_signaling_pathway_hsa04022 2 163 0.6664 0.8885
36 Calcium_signaling_pathway_hsa04020 2 182 0.7242 0.9034
37 Cytokine_cytokine_receptor_interaction_hsa04060 3 270 0.7296 0.9034
38 Neuroactive_ligand_receptor_interaction_hsa04080 3 278 0.7476 0.904

Quest ID: 6a1678764a4e9fc2f36016de5ed32647