This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are
presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.
"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.
Num | microRNA | Gene | miRNA log2FC | miRNA pvalue | Gene log2FC | Gene pvalue | Interaction | Correlation beta | Correlation P-value | PMID | Reported in cancer studies |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | hsa-miR-107 | PGK1 | 0.24 | 0.01708 | 0.22 | 0.04286 | miRanda | -0.19 | 0.00034 | NA | |
2 | hsa-miR-130a-3p | PGK1 | -1.53 | 0 | 0.22 | 0.04286 | mirMAP | -0.16 | 0 | NA | |
3 | hsa-miR-193a-3p | PGK1 | -0.12 | 0.30939 | 0.22 | 0.04286 | miRanda | -0.18 | 6.0E-5 | NA | |
4 | hsa-miR-195-3p | PGK1 | -1.09 | 0 | 0.22 | 0.04286 | mirMAP | -0.1 | 0.00022 | NA | |
5 | hsa-miR-20a-5p | PGK1 | 0.85 | 0 | 0.22 | 0.04286 | miRNAWalker2 validate | -0.15 | 0 | NA |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | ESTABLISHMENT OF PROTEIN LOCALIZATION TO ENDOPLASMIC RETICULUM | 16 | 104 | 4.162e-24 | 1.937e-20 |
2 | NUCLEAR TRANSCRIBED MRNA CATABOLIC PROCESS NONSENSE MEDIATED DECAY | 16 | 118 | 3.554e-23 | 8.269e-20 |
3 | PROTEIN LOCALIZATION TO ENDOPLASMIC RETICULUM | 16 | 123 | 7.155e-23 | 1.11e-19 |
4 | MULTI ORGANISM METABOLIC PROCESS | 16 | 138 | 4.916e-22 | 5.718e-19 |
5 | TRANSLATIONAL INITIATION | 16 | 146 | 1.255e-21 | 1.168e-18 |
6 | PROTEIN TARGETING TO MEMBRANE | 16 | 157 | 4.179e-21 | 3.241e-18 |
7 | RNA CATABOLIC PROCESS | 17 | 227 | 4.394e-20 | 2.921e-17 |
8 | RRNA METABOLIC PROCESS | 17 | 255 | 3.223e-19 | 1.875e-16 |
9 | RIBOSOME BIOGENESIS | 17 | 308 | 7.942e-18 | 4.106e-15 |
10 | ESTABLISHMENT OF PROTEIN LOCALIZATION TO MEMBRANE | 16 | 264 | 1.923e-17 | 8.946e-15 |
11 | INTERSPECIES INTERACTION BETWEEN ORGANISMS | 21 | 662 | 4.212e-17 | 1.633e-14 |
12 | SYMBIOSIS ENCOMPASSING MUTUALISM THROUGH PARASITISM | 21 | 662 | 4.212e-17 | 1.633e-14 |
13 | VIRAL LIFE CYCLE | 16 | 290 | 8.569e-17 | 2.861e-14 |
14 | ORGANIC CYCLIC COMPOUND CATABOLIC PROCESS | 18 | 427 | 8.608e-17 | 2.861e-14 |
15 | MRNA METABOLIC PROCESS | 20 | 611 | 1.555e-16 | 4.823e-14 |
16 | NCRNA PROCESSING | 17 | 386 | 3.48e-16 | 1.012e-13 |
17 | ESTABLISHMENT OF PROTEIN LOCALIZATION TO ORGANELLE | 16 | 361 | 2.697e-15 | 7.381e-13 |
18 | RIBONUCLEOPROTEIN COMPLEX BIOGENESIS | 17 | 440 | 3.038e-15 | 7.852e-13 |
19 | PROTEIN LOCALIZATION TO MEMBRANE | 16 | 376 | 5.092e-15 | 1.247e-12 |
20 | PROTEIN TARGETING | 16 | 406 | 1.68e-14 | 3.908e-12 |
21 | AMIDE BIOSYNTHETIC PROCESS | 17 | 507 | 3.098e-14 | 6.863e-12 |
22 | RNA PROCESSING | 20 | 835 | 5.813e-14 | 1.229e-11 |
23 | NCRNA METABOLIC PROCESS | 17 | 533 | 6.986e-14 | 1.413e-11 |
24 | PEPTIDE METABOLIC PROCESS | 17 | 571 | 2.13e-13 | 4.129e-11 |
25 | ORGANONITROGEN COMPOUND BIOSYNTHETIC PROCESS | 21 | 1024 | 2.352e-13 | 4.378e-11 |
26 | CELLULAR AMIDE METABOLIC PROCESS | 18 | 727 | 8.195e-13 | 1.467e-10 |
27 | PROTEIN LOCALIZATION TO ORGANELLE | 16 | 556 | 2.076e-12 | 3.577e-10 |
28 | CATABOLIC PROCESS | 25 | 1773 | 2.523e-12 | 4.193e-10 |
29 | ORGANONITROGEN COMPOUND METABOLIC PROCESS | 24 | 1796 | 2.635e-11 | 4.228e-09 |
30 | SINGLE ORGANISM CELLULAR LOCALIZATION | 18 | 898 | 2.757e-11 | 4.276e-09 |
31 | MEMBRANE ORGANIZATION | 17 | 899 | 2.754e-10 | 4.134e-08 |
32 | INTRACELLULAR PROTEIN TRANSPORT | 16 | 781 | 3.25e-10 | 4.726e-08 |
33 | MACROMOLECULE CATABOLIC PROCESS | 17 | 926 | 4.339e-10 | 6.118e-08 |
34 | CELLULAR CATABOLIC PROCESS | 19 | 1322 | 1.916e-09 | 2.622e-07 |
35 | ESTABLISHMENT OF LOCALIZATION IN CELL | 20 | 1676 | 1.532e-08 | 2.037e-06 |
36 | CELLULAR MACROMOLECULE LOCALIZATION | 16 | 1234 | 2.087e-07 | 2.697e-05 |
37 | HEXOSE CATABOLIC PROCESS | 5 | 49 | 2.723e-07 | 3.424e-05 |
38 | HEXOSE METABOLIC PROCESS | 7 | 157 | 3.055e-07 | 3.741e-05 |
39 | MONOSACCHARIDE CATABOLIC PROCESS | 5 | 59 | 6.996e-07 | 8.346e-05 |
40 | ESTABLISHMENT OF PROTEIN LOCALIZATION | 16 | 1423 | 1.407e-06 | 0.0001636 |
41 | MONOSACCHARIDE METABOLIC PROCESS | 7 | 202 | 1.666e-06 | 0.0001891 |
42 | SMALL MOLECULE CATABOLIC PROCESS | 8 | 328 | 3.947e-06 | 0.0004372 |
43 | CYTOPLASMIC TRANSLATION | 4 | 41 | 5.549e-06 | 0.0006005 |
44 | CARBOHYDRATE CATABOLIC PROCESS | 5 | 113 | 1.745e-05 | 0.001845 |
45 | GLUCOSE METABOLIC PROCESS | 5 | 119 | 2.241e-05 | 0.002317 |
46 | PROTEIN LOCALIZATION | 16 | 1805 | 2.928e-05 | 0.002962 |
47 | GLUCOSE CATABOLIC PROCESS | 3 | 29 | 7.609e-05 | 0.007533 |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | STRUCTURAL CONSTITUENT OF RIBOSOME | 16 | 212 | 5.662e-19 | 5.26e-16 |
2 | RNA BINDING | 23 | 1598 | 1.829e-11 | 8.493e-09 |
3 | STRUCTURAL MOLECULE ACTIVITY | 16 | 732 | 1.257e-10 | 3.894e-08 |
4 | POLY A RNA BINDING | 18 | 1170 | 1.986e-09 | 4.611e-07 |
5 | MAGNESIUM ION BINDING | 6 | 199 | 2.141e-05 | 0.003472 |
6 | RRNA BINDING | 4 | 58 | 2.243e-05 | 0.003472 |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | CYTOSOLIC RIBOSOME | 16 | 113 | 1.709e-23 | 9.978e-21 |
2 | RIBOSOMAL SUBUNIT | 16 | 163 | 7.757e-21 | 2.265e-18 |
3 | CYTOSOLIC PART | 17 | 223 | 3.236e-20 | 6.299e-18 |
4 | RIBOSOME | 16 | 226 | 1.59e-18 | 2.322e-16 |
5 | CYTOSOLIC LARGE RIBOSOMAL SUBUNIT | 10 | 61 | 1.24e-15 | 1.448e-13 |
6 | RIBONUCLEOPROTEIN COMPLEX | 19 | 721 | 5.313e-14 | 5.172e-12 |
7 | LARGE RIBOSOMAL SUBUNIT | 10 | 95 | 1.292e-13 | 1.078e-11 |
8 | CYTOSOLIC SMALL RIBOSOMAL SUBUNIT | 6 | 44 | 2.654e-09 | 1.937e-07 |
9 | CELL SUBSTRATE JUNCTION | 11 | 398 | 1.371e-08 | 8.896e-07 |
10 | SMALL RIBOSOMAL SUBUNIT | 6 | 68 | 3.904e-08 | 2.28e-06 |
11 | ANCHORING JUNCTION | 11 | 489 | 1.116e-07 | 5.922e-06 |
Num | Pathway | Pathview | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|---|
1 | PI3K_Akt_signaling_pathway_hsa04151 | 5 | 352 | 0.003249 | 0.1649 | |
2 | FoxO_signaling_pathway_hsa04068 | 3 | 132 | 0.006341 | 0.1649 | |
3 | p53_signaling_pathway_hsa04115 | 2 | 68 | 0.01612 | 0.2794 | |
4 | HIF_1_signaling_pathway_hsa04066 | 2 | 100 | 0.03306 | 0.4297 | |
5 | AMPK_signaling_pathway_hsa04152 | 2 | 121 | 0.04669 | 0.4474 | |
6 | Autophagy_animal_hsa04140 | 2 | 128 | 0.05162 | 0.4474 | |
7 | mTOR_signaling_pathway_hsa04150 | 2 | 151 | 0.06903 | 0.46 | |
8 | Cellular_senescence_hsa04218 | 2 | 160 | 0.0763 | 0.46 | |
9 | Necroptosis_hsa04217 | 2 | 164 | 0.07961 | 0.46 | |
10 | MAPK_signaling_pathway_hsa04010 | 2 | 295 | 0.2054 | 0.89 |