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This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.

"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.

miRNA-gene regulations

(Download full result)

Num microRNA           Gene miRNA log2FC miRNA pvalue Gene log2FC Gene pvalue Interaction Correlation beta Correlation P-value PMID Reported in cancer studies
1 hsa-miR-27b-3p ABI2 0.2 0.29563 -0.65 0 mirMAP -0.11 0.00065 NA
2 hsa-miR-27b-3p ABL2 0.2 0.29563 -0.32 0.04554 MirTarget; miRNATAP -0.12 0.00382 NA
3 hsa-miR-27b-3p ADAMTS6 0.2 0.29563 -0.88 0.01914 miRNATAP -0.25 0.01316 NA
4 hsa-miR-27b-3p ADCY3 0.2 0.29563 -0.09 0.61168 miRNATAP -0.15 0.00138 NA
5 hsa-miR-27b-3p AFAP1 0.2 0.29563 -0.98 0.00012 miRNATAP -0.16 0.02042 NA
6 hsa-miR-27b-3p ANKRD36B 0.2 0.29563 0.26 0.50637 MirTarget -0.29 0.00388 NA
7 hsa-miR-27b-3p ANTXR2 0.2 0.29563 -2.31 0 miRNATAP -0.26 0.00239 NA
8 hsa-miR-27b-3p AQP11 0.2 0.29563 0.61 0.01241 MirTarget; miRNATAP -0.18 0.00473 NA
9 hsa-miR-27b-3p ARHGEF6 0.2 0.29563 -1.32 0 MirTarget -0.25 0.00093 NA
10 hsa-miR-27b-3p ARNTL 0.2 0.29563 -0.36 0.08956 miRNATAP -0.15 0.00499 NA
11 hsa-miR-27b-3p ATP11C 0.2 0.29563 -0.48 0.00363 MirTarget; miRNATAP -0.15 0.00073 NA
12 hsa-miR-27b-3p BRPF3 0.2 0.29563 0.03 0.87192 MirTarget; miRNATAP -0.13 0.00202 NA
13 hsa-miR-27b-3p BRSK1 0.2 0.29563 -1.19 0.00301 MirTarget; miRNATAP -0.21 0.04965 NA
14 hsa-miR-27b-3p CALM3 0.2 0.29563 -0.26 0.0214 miRNAWalker2 validate; miRNATAP -0.1 0.00053 NA
15 hsa-miR-27b-3p CBX5 0.2 0.29563 -0.14 0.49023 miRNATAP -0.12 0.01793 NA
16 hsa-miR-27b-3p CCDC50 0.2 0.29563 -0.78 0 mirMAP -0.16 4.0E-5 NA
17 hsa-miR-27b-3p CCM2 0.2 0.29563 0.5 0.00153 MirTarget; miRNATAP -0.13 0.00182 NA
18 hsa-miR-27b-3p CCND3 0.2 0.29563 0.14 0.39731 miRNAWalker2 validate -0.13 0.00231 NA
19 hsa-miR-27b-3p CD28 0.2 0.29563 -1.38 0.00084 miRNATAP -0.22 0.039 NA
20 hsa-miR-27b-3p CD84 0.2 0.29563 -0.53 0.27947 mirMAP -0.32 0.01228 NA
21 hsa-miR-27b-3p CDC25B 0.2 0.29563 0.78 0.00479 miRNATAP -0.18 0.01146 NA
22 hsa-miR-27b-3p CPPED1 0.2 0.29563 -0.72 0.0334 MirTarget -0.28 0.00165 NA
23 hsa-miR-27b-3p CRTC1 0.2 0.29563 -0.42 0.0113 miRNATAP -0.1 0.01965 NA
24 hsa-miR-27b-3p CSF1 0.2 0.29563 -1.3 0.00033 MirTarget; miRNATAP -0.39 3.0E-5 NA
25 hsa-miR-27b-3p DCHS2 0.2 0.29563 -3.63 0 MirTarget -0.33 0.01491 NA
26 hsa-miR-27b-3p DLL4 0.2 0.29563 -0.14 0.54558 miRNATAP -0.12 0.03891 NA
27 hsa-miR-27b-3p EIF2AK2 0.2 0.29563 0.89 1.0E-5 mirMAP -0.17 0.00217 NA
28 hsa-miR-27b-3p FAM120C 0.2 0.29563 -0.22 0.20934 mirMAP; miRNATAP -0.13 0.00471 NA
29 hsa-miR-27b-3p FAM184A 0.2 0.29563 -1.88 1.0E-5 MirTarget -0.38 0.00094 NA
30 hsa-miR-27b-3p FAM65B 0.2 0.29563 -1.56 5.0E-5 miRNATAP -0.26 0.0102 NA
31 hsa-miR-27b-3p FAM78A 0.2 0.29563 -0.18 0.57167 MirTarget; miRNATAP -0.23 0.00491 NA
32 hsa-miR-27b-3p FRS3 0.2 0.29563 0.41 0.00977 MirTarget; miRNATAP -0.13 0.00117 NA
33 hsa-miR-27b-3p FYN 0.2 0.29563 -1.47 0 miRNATAP -0.3 5.0E-5 NA
34 hsa-miR-27b-3p FZD4 0.2 0.29563 -0.83 0.00068 miRNATAP -0.18 0.00464 NA
35 hsa-miR-27b-3p GNAZ 0.2 0.29563 -2.95 0 miRNATAP -0.27 0.03825 NA
36 hsa-miR-27b-3p GNS 0.2 0.29563 -0.11 0.4674 miRNATAP -0.11 0.00325 NA
37 hsa-miR-27b-3p GOLM1 0.2 0.29563 0.83 0.00016 MirTarget -0.15 0.01091 25115396 GOLM1 was directly regulated by miR-27b in PCa cells
38 hsa-miR-27b-3p GPR180 0.2 0.29563 0.73 0 mirMAP -0.12 0.00472 NA
39 hsa-miR-27b-3p H2AFZ 0.2 0.29563 0.71 1.0E-5 miRNATAP -0.11 0.01074 NA
40 hsa-miR-27b-3p HDX 0.2 0.29563 -1.08 0.00226 miRNATAP -0.26 0.00474 NA
41 hsa-miR-27b-3p HIP1 0.2 0.29563 -0.47 0.05549 mirMAP; miRNATAP -0.2 0.00199 NA
42 hsa-miR-27b-3p HIPK2 0.2 0.29563 -0.57 0.01764 miRNATAP -0.16 0.01162 NA
43 hsa-miR-27b-3p HIVEP3 0.2 0.29563 0.03 0.92033 MirTarget; miRNATAP -0.32 2.0E-5 NA
44 hsa-miR-27b-3p IKZF1 0.2 0.29563 -1.31 0.0016 miRNATAP -0.31 0.00483 NA
45 hsa-miR-27b-3p IQCE 0.2 0.29563 0 0.99828 mirMAP -0.12 0.0075 NA
46 hsa-miR-27b-3p IQGAP2 0.2 0.29563 -1.52 3.0E-5 MirTarget -0.3 0.00175 NA
47 hsa-miR-27b-3p JAZF1 0.2 0.29563 -1.92 0 miRNATAP -0.16 0.02811 NA
48 hsa-miR-27b-3p KATNA1 0.2 0.29563 -0.24 0.04591 MirTarget -0.11 0.00032 NA
49 hsa-miR-27b-3p KCTD14 0.2 0.29563 0.9 0.03752 MirTarget -0.6 0 NA
50 hsa-miR-27b-3p KIF21B 0.2 0.29563 0.41 0.12151 miRNATAP -0.16 0.02076 NA
51 hsa-miR-27b-3p KLF12 0.2 0.29563 -1.47 1.0E-5 mirMAP -0.21 0.0188 NA
52 hsa-miR-27b-3p KLHDC3 0.2 0.29563 0.58 3.0E-5 miRNATAP -0.1 0.00454 NA
53 hsa-miR-27b-3p LBR 0.2 0.29563 -0.52 0.00086 MirTarget -0.21 0 NA
54 hsa-miR-27b-3p LIFR 0.2 0.29563 -2.63 0 MirTarget; miRNATAP -0.33 0.003 NA
55 hsa-miR-27b-3p LPIN1 0.2 0.29563 -0.9 0.00013 miRNATAP -0.23 0.00021 NA
56 hsa-miR-27b-3p MAP3K12 0.2 0.29563 -0.86 0.00031 miRNATAP -0.17 0.00685 NA
57 hsa-miR-27b-3p MARCH1 0.2 0.29563 0.25 0.34759 miRNATAP -0.22 0.00145 NA
58 hsa-miR-27b-3p MEF2C 0.2 0.29563 -2.13 0 miRNATAP -0.24 0.00143 NA
59 hsa-miR-27b-3p MMD 0.2 0.29563 -0.05 0.87055 miRNATAP -0.27 0.00085 NA
60 hsa-miR-27b-3p NAV1 0.2 0.29563 -0.42 0.10874 miRNATAP -0.18 0.00855 NA
61 hsa-miR-27b-3p NCOA7 0.2 0.29563 -1.35 0 miRNATAP -0.15 0.03204 NA
62 hsa-miR-27b-3p NDUFS6 0.2 0.29563 0.69 8.0E-5 miRNAWalker2 validate -0.1 0.02385 NA
63 hsa-miR-27b-3p NFIB 0.2 0.29563 -1.73 0 mirMAP; miRNATAP -0.21 0.00844 NA
64 hsa-miR-27b-3p NR5A2 0.2 0.29563 -1.31 0 miRNATAP -0.33 1.0E-5 NA
65 hsa-miR-27b-3p OAF 0.2 0.29563 -0.85 5.0E-5 MirTarget -0.13 0.01992 NA
66 hsa-miR-27b-3p ORAI2 0.2 0.29563 0.42 0.02257 mirMAP -0.12 0.01323 NA
67 hsa-miR-27b-3p PALM2 0.2 0.29563 0.56 0.12712 MirTarget -0.31 0.00099 NA
68 hsa-miR-27b-3p PAX6 0.2 0.29563 -0.04 0.8927 mirMAP -0.3 0.00048 NA
69 hsa-miR-27b-3p PDHX 0.2 0.29563 -0.16 0.21989 MirTarget; miRNATAP -0.11 0.00129 NA
70 hsa-miR-27b-3p PDK4 0.2 0.29563 -4.43 0 MirTarget -0.3 0.01778 NA
71 hsa-miR-27b-3p PDS5B 0.2 0.29563 -0.33 0.02299 MirTarget; miRNATAP -0.11 0.00293 NA
72 hsa-miR-27b-3p PHLPP2 0.2 0.29563 -0.54 0.00199 MirTarget; miRNATAP -0.12 0.00955 NA
73 hsa-miR-27b-3p PLCL2 0.2 0.29563 -0.71 0.04741 miRNATAP -0.35 0.00016 NA
74 hsa-miR-27b-3p PLXND1 0.2 0.29563 -0.25 0.28121 MirTarget; miRNATAP -0.21 0.00052 NA
75 hsa-miR-27b-3p PODXL 0.2 0.29563 0.04 0.86377 mirMAP -0.21 0.00098 NA
76 hsa-miR-27b-3p PSMA1 0.2 0.29563 0.28 0.01028 MirTarget -0.12 2.0E-5 NA
77 hsa-miR-27b-3p RFX3 0.2 0.29563 -0.24 0.32908 mirMAP; miRNATAP -0.16 0.01293 NA
78 hsa-miR-27b-3p RNF24 0.2 0.29563 0.57 0.00299 mirMAP -0.11 0.03361 NA
79 hsa-miR-27b-3p ROR1 0.2 0.29563 -3.31 0 MirTarget; miRNATAP -0.32 0.02024 26576539 Bioinformatic prediction luciferase reporter assay quantitative real-time PCR qRT-PCR and western blotting were used to reveal the regulatory relationship between miR-27b-3p and ROR1; The expression patterns of miR-27b-3p and ROR1 in human gastric cancer GC specimens and cell lines were determined by microRNA RT-PCR and western blotting; miR-27b-3p suppressed ROR1 expression by binding to the 3'UTR of ROR1 mRNA in GC cells; miR-27b-3p was significantly downregulated and reversely correlated with ROR1 protein levels in clinical samples; Analysis of the clinicopathological significance showed that miR-27b-3p and ROR1 were closely correlated with GC characteristics; These results show that miR-27b-3p suppresses ROR1 expression through the binding site in the 3'UTR inhibiting the cell proliferation; These findings indicate that miR-27b-3p exerts tumor-suppressive effects in GC through the suppression of oncogene ROR1 expression and suggest a therapeutic application of miR-27b-3p in GC
80 hsa-miR-27b-3p RSAD2 0.2 0.29563 1.09 0.01833 MirTarget -0.37 0.00248 NA
81 hsa-miR-27b-3p SGTB 0.2 0.29563 -0.74 0.0018 MirTarget -0.16 0.00872 NA
82 hsa-miR-27b-3p SH2D3C 0.2 0.29563 -0.69 0.00308 miRNATAP -0.19 0.00172 NA
83 hsa-miR-27b-3p SLC39A13 0.2 0.29563 0.24 0.13007 miRNATAP -0.15 0.00019 NA
84 hsa-miR-27b-3p SLC43A1 0.2 0.29563 -1.49 1.0E-5 miRNAWalker2 validate -0.24 0.00624 NA
85 hsa-miR-27b-3p SLC8A1 0.2 0.29563 -2.45 0 MirTarget -0.24 0.01181 NA
86 hsa-miR-27b-3p SLFN11 0.2 0.29563 -0.53 0.1537 MirTarget -0.29 0.00311 NA
87 hsa-miR-27b-3p SLFN5 0.2 0.29563 -0.76 0.00885 MirTarget -0.19 0.01266 NA
88 hsa-miR-27b-3p SNN 0.2 0.29563 -0.43 0.02698 miRNATAP -0.1 0.04598 NA
89 hsa-miR-27b-3p SNX10 0.2 0.29563 1.54 0 miRNATAP -0.48 0 NA
90 hsa-miR-27b-3p SPATA13 0.2 0.29563 -0.77 0.04293 miRNATAP -0.21 0.03437 NA
91 hsa-miR-27b-3p ST6GALNAC3 0.2 0.29563 -2.05 0 MirTarget -0.17 0.04381 NA
92 hsa-miR-27b-3p TEAD1 0.2 0.29563 -1.49 0 miRNATAP -0.14 0.00999 NA
93 hsa-miR-27b-3p TMEM170B 0.2 0.29563 -0.89 0.00247 MirTarget; miRNATAP -0.2 0.00814 NA
94 hsa-miR-27b-3p TMSB10 0.2 0.29563 1.1 0 miRNATAP -0.19 0.00177 NA
95 hsa-miR-27b-3p TNFRSF1B 0.2 0.29563 -0.73 0.0116 mirMAP -0.23 0.00266 NA
96 hsa-miR-27b-3p TRIM21 0.2 0.29563 0.09 0.65404 MirTarget -0.22 2.0E-5 NA
97 hsa-miR-27b-3p USP13 0.2 0.29563 -0.64 0.00602 mirMAP -0.25 5.0E-5 NA
98 hsa-miR-27b-3p USP51 0.2 0.29563 -1.32 0.00017 MirTarget; miRNATAP -0.28 0.00252 NA
99 hsa-miR-27b-3p VEGFC 0.2 0.29563 -0.46 0.17861 MirTarget -0.18 0.03822 23593282 We identified vascular endothelial growth factor C VEGFC as a novel target gene of miR-27b and determined that miR-27b functioned as an inhibitor of tumor progression and angiogenesis through targeting VEGFC in CRC; In summary an anti-tumor role for miR-27b and its novel target VEGFC in vivo could lead to tumor necrosis and provide a rationale for developing miR-27b as a therapeutic agent
100 hsa-miR-27b-3p WDR35 0.2 0.29563 -0.41 0.01575 MirTarget -0.12 0.00513 NA
101 hsa-miR-27b-3p ZC3H12D 0.2 0.29563 -0.17 0.68046 MirTarget -0.28 0.00831 NA
102 hsa-miR-27b-3p ZEB2 0.2 0.29563 -2.26 0 miRNATAP -0.26 0.00241 NA

Over-represented Gene Ontology Biological Process

NumGOOverlapSizeP ValueAdj. P Value
1 POSITIVE REGULATION OF MOLECULAR FUNCTION 25 1791 2.56e-06 0.005109
2 PROTEIN PHOSPHORYLATION 18 944 1.226e-06 0.005109
3 PHOSPHORYLATION 20 1228 3.294e-06 0.005109
NumGOOverlapSizeP ValueAdj. P Value
NumGOOverlapSizeP ValueAdj. P Value

Over-represented Pathway

NumPathwayPathviewOverlapSizeP ValueAdj. P Value
1 Apelin_signaling_pathway_hsa04371 4 137 0.005334 0.1424
2 Hippo_signaling_pathway_multiple_species_hsa04392 2 29 0.00956 0.1424
3 cGMP_PKG_signaling_pathway_hsa04022 4 163 0.00974 0.1424
4 Calcium_signaling_pathway_hsa04020 4 182 0.01413 0.1424
5 MAPK_signaling_pathway_hsa04010 5 295 0.01762 0.1424
6 TNF_signaling_pathway_hsa04668 3 108 0.01787 0.1424
7 Rap1_signaling_pathway_hsa04015 4 206 0.02124 0.1424
8 Regulation_of_actin_cytoskeleton_hsa04810 4 208 0.02191 0.1424
9 Ras_signaling_pathway_hsa04014 4 232 0.03105 0.1782
10 Signaling_pathways_regulating_pluripotency_of_stem_cells_hsa04550 3 139 0.03427 0.1782
11 Hippo_signaling_pathway_hsa04390 3 154 0.04425 0.198
12 Cellular_senescence_hsa04218 3 160 0.0486 0.198
13 Cytokine_cytokine_receptor_interaction_hsa04060 4 270 0.0495 0.198
14 Focal_adhesion_hsa04510 3 199 0.08167 0.3033
15 PI3K_Akt_signaling_pathway_hsa04151 4 352 0.1057 0.3665
16 Cell_cycle_hsa04110 2 124 0.132 0.4037
17 Oocyte_meiosis_hsa04114 2 124 0.132 0.4037
18 Phospholipase_D_signaling_pathway_hsa04072 2 146 0.1709 0.4677
19 Wnt_signaling_pathway_hsa04310 2 146 0.1709 0.4677
20 mTOR_signaling_pathway_hsa04150 2 151 0.18 0.4679
21 Jak_STAT_signaling_pathway_hsa04630 2 162 0.2002 0.482
22 Necroptosis_hsa04217 2 164 0.2039 0.482
23 cAMP_signaling_pathway_hsa04024 2 198 0.2679 0.5804

Quest ID: 9f35120b626e2828d83ac2c5e1d8037e