Explanation
This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are
presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.
"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.
miRNA-gene regulations
| Num | microRNA | Gene | miRNA log2FC | miRNA pvalue | Gene log2FC | Gene pvalue | Interaction | Correlation beta | Correlation P-value | PMID | Reported in cancer studies |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | hsa-miR-221-3p | AMOT | 1.12 | 0 | -0.9 | 0.00079 | miRNAWalker2 validate | -0.32 | 9.0E-5 | NA | |
| 2 | hsa-miR-221-3p | AQP3 | 1.12 | 0 | -1.9 | 0 | MirTarget | -0.17 | 0.00795 | NA | |
| 3 | hsa-miR-221-3p | ARAP2 | 1.12 | 0 | -1.13 | 0 | MirTarget | -0.14 | 0.00818 | NA | |
| 4 | hsa-miR-221-3p | ARHGAP42 | 1.12 | 0 | -1.24 | 0 | miRNAWalker2 validate; MirTarget; miRNATAP | -0.4 | 0 | NA | |
| 5 | hsa-miR-221-3p | ASB4 | 1.12 | 0 | -0.62 | 0.06683 | MirTarget | -0.34 | 0.00111 | NA | |
| 6 | hsa-miR-221-3p | ASB7 | 1.12 | 0 | -0.2 | 0.00927 | miRNATAP | -0.1 | 1.0E-5 | NA | |
| 7 | hsa-miR-221-3p | ASPA | 1.12 | 0 | -2.88 | 0 | MirTarget | -0.66 | 0 | NA | |
| 8 | hsa-miR-221-3p | ASXL3 | 1.12 | 0 | -2.69 | 0 | miRNAWalker2 validate | -0.95 | 0 | NA | |
| 9 | hsa-miR-221-3p | ATL2 | 1.12 | 0 | -0.1 | 0.23951 | miRNAWalker2 validate | -0.1 | 5.0E-5 | NA | |
| 10 | hsa-miR-221-3p | BNIP3 | 1.12 | 0 | -0.57 | 1.0E-5 | miRNAWalker2 validate | -0.19 | 0 | NA | |
| 11 | hsa-miR-221-3p | BNIP3L | 1.12 | 0 | -0.67 | 0 | miRNAWalker2 validate; miRTarBase | -0.12 | 0.00023 | NA | |
| 12 | hsa-miR-221-3p | BRWD1 | 1.12 | 0 | -0.47 | 0 | MirTarget | -0.14 | 0 | NA | |
| 13 | hsa-miR-221-3p | CAMTA1 | 1.12 | 0 | -0.31 | 0.0011 | MirTarget | -0.13 | 1.0E-5 | NA | |
| 14 | hsa-miR-221-3p | CASR | 1.12 | 0 | -3.09 | 0 | MirTarget | -0.39 | 0.00241 | NA | |
| 15 | hsa-miR-221-3p | CBWD3 | 1.12 | 0 | -0.68 | 0 | MirTarget | -0.16 | 0.00013 | NA | |
| 16 | hsa-miR-221-3p | CD4 | 1.12 | 0 | -2.2 | 0 | MirTarget; miRNATAP | -0.35 | 0 | NA | |
| 17 | hsa-miR-221-3p | CDKN1B | 1.12 | 0 | -0.23 | 0.00482 | miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP | -0.15 | 0 | 20146005; 23637992; 19953484; 23939688; 19126397; 23967190; 17569667; 22992757; 17721077; 20461750 | Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression |
| 18 | hsa-miR-221-3p | CLN8 | 1.12 | 0 | -0.9 | 0 | mirMAP | -0.14 | 0.00057 | NA | |
| 19 | hsa-miR-221-3p | CNRIP1 | 1.12 | 0 | -0.55 | 3.0E-5 | miRNAWalker2 validate | -0.12 | 0.0033 | NA | |
| 20 | hsa-miR-221-3p | CPNE8 | 1.12 | 0 | -0.76 | 0 | MirTarget; miRNATAP | -0.3 | 0 | NA | |
| 21 | hsa-miR-221-3p | CREBL2 | 1.12 | 0 | -0.86 | 0 | MirTarget | -0.26 | 0 | NA | |
| 22 | hsa-miR-221-3p | CXCL12 | 1.12 | 0 | -3.59 | 0 | MirTarget | -0.31 | 0.00118 | NA | |
| 23 | hsa-miR-221-3p | CYP1B1 | 1.12 | 0 | -0.08 | 0.76097 | miRNAWalker2 validate; MirTarget | -0.24 | 0.00202 | NA | |
| 24 | hsa-miR-221-3p | DCUN1D1 | 1.12 | 0 | -0.45 | 3.0E-5 | MirTarget | -0.18 | 0 | NA | |
| 25 | hsa-miR-221-3p | DCUN1D4 | 1.12 | 0 | -0.24 | 0.00352 | MirTarget | -0.1 | 7.0E-5 | NA | |
| 26 | hsa-miR-221-3p | DDAH1 | 1.12 | 0 | -0.92 | 0 | miRNAWalker2 validate | -0.23 | 0 | NA | |
| 27 | hsa-miR-221-3p | DDX3X | 1.12 | 0 | -0.7 | 0 | miRNATAP | -0.1 | 0.0001 | NA | |
| 28 | hsa-miR-221-3p | DGKE | 1.12 | 0 | -0.86 | 0 | miRNATAP | -0.36 | 0 | NA | |
| 29 | hsa-miR-221-3p | DIRAS3 | 1.12 | 0 | -4.17 | 0 | miRTarBase | -0.99 | 0 | 21071579; 23801152; 22117988 | Further studies on a new mechanism of ARHI downregulation showed a significant inverse relationship between ARHI and miR-221 and 222 which were upregulated in prostate cancer cell lines; Transfection of miR-221 and 222 inhibitors into PC-3 cells caused a significant induction of ARHI expression; A direct interaction of miR-221 or 222 with a target site on the 3'UTR of ARHI was confirmed by a dual luciferase pMIR-REPORT assay; Finally we also found that genistein upregulates ARHI by downregulating miR-221 and 222 in PC-3 cells;Effects of ARHI on breast cancer cell biological behavior regulated by microRNA 221; The downregulation of ARHI was regulated by miR-221 in prostate cancer cell lines; However it has not been reported whether ARHI is regulated by miR-221 in breast cancer; To address whether ARHI is regulated by miR-221 in breast cancer cell lines the results in this study showed that a significant inverse correlation existed between ARHI and miR-221; The inhibition of miR-221 induced a significant upregulation of ARHI in MCF-7 cells; To prove a direct interaction between miR-221 and ARHI mRNA ARHI 3'UTR which includes the potential target site for miR-221 was cloned downstream of the luciferase reporter gene of the pMIR-REPORT vector to generate the pMIR-ARHI-3'UTR vector; The results confirmed a direct interaction of miR-221 with a target site on the 3'UTR of ARHI; This study demonstrated for the first time that the downregulation of ARHI in breast cancer cells could be regulated by miR-221;MicroRNA 221 and 222 have been shown to regulate ARHI expression negatively; Expression of ARHI and microRNA 221 and 222 was measured by real-time reverse transcription-polymerase chain reaction; Whether ARHI and microRNA 221 and 222 could be considered as biomarkers for disease progression in prostate cancer requires further investigation |
| 30 | hsa-miR-221-3p | DPP8 | 1.12 | 0 | -0.27 | 0.06918 | MirTarget | -0.1 | 0.02426 | NA | |
| 31 | hsa-miR-221-3p | EIF4E3 | 1.12 | 0 | -1.66 | 0 | miRNATAP | -0.36 | 0 | NA | |
| 32 | hsa-miR-221-3p | ERCC4 | 1.12 | 0 | -0.4 | 0.00032 | MirTarget | -0.24 | 0 | NA | |
| 33 | hsa-miR-221-3p | ESR1 | 1.12 | 0 | -4.12 | 0 | miRNAWalker2 validate; miRNATAP | -1.1 | 0 | 25483016 | HBx protein induced upregulation of microRNA 221 promotes aberrant proliferation in HBV‑related hepatocellular carcinoma by targeting estrogen receptor α |
| 34 | hsa-miR-221-3p | ETS1 | 1.12 | 0 | -0.89 | 0 | miRTarBase; miRNATAP | -0.16 | 0.00028 | 21711453 | To close the loop we demonstrate ETS-1 as a direct target of miR-222 but not miR-221 showing the novel option of their uncoupled functions |
| 35 | hsa-miR-221-3p | ETS2 | 1.12 | 0 | -1.76 | 0 | miRNATAP | -0.36 | 0 | NA | |
| 36 | hsa-miR-221-3p | FAM13A | 1.12 | 0 | -2.58 | 0 | miRNATAP | -0.57 | 0 | NA | |
| 37 | hsa-miR-221-3p | FERMT2 | 1.12 | 0 | -1.17 | 0 | MirTarget; miRNATAP | -0.15 | 3.0E-5 | NA | |
| 38 | hsa-miR-221-3p | FKBP5 | 1.12 | 0 | -0.23 | 0.1942 | mirMAP | -0.22 | 6.0E-5 | NA | |
| 39 | hsa-miR-221-3p | FNDC3A | 1.12 | 0 | -1 | 0 | MirTarget; miRNATAP | -0.14 | 0.00081 | NA | |
| 40 | hsa-miR-221-3p | FOS | 1.12 | 0 | -3.64 | 0 | miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP | -0.56 | 0 | NA | |
| 41 | hsa-miR-221-3p | FXN | 1.12 | 0 | -1.38 | 0 | MirTarget | -0.16 | 0.00012 | NA | |
| 42 | hsa-miR-221-3p | GAB1 | 1.12 | 0 | -0.32 | 0.00456 | MirTarget; miRNATAP | -0.14 | 3.0E-5 | NA | |
| 43 | hsa-miR-221-3p | GLYR1 | 1.12 | 0 | -0.17 | 0.02754 | miRNAWalker2 validate | -0.11 | 1.0E-5 | NA | |
| 44 | hsa-miR-221-3p | GPM6A | 1.12 | 0 | -4.85 | 0 | miRNATAP | -0.87 | 0 | NA | |
| 45 | hsa-miR-221-3p | GUCY1A2 | 1.12 | 0 | 0.07 | 0.67688 | MirTarget | -0.21 | 5.0E-5 | NA | |
| 46 | hsa-miR-221-3p | HECTD1 | 1.12 | 0 | -0.51 | 0 | miRNAWalker2 validate | -0.12 | 6.0E-5 | NA | |
| 47 | hsa-miR-221-3p | HIPK1 | 1.12 | 0 | -0.16 | 0.1272 | MirTarget; miRNATAP | -0.11 | 0.00035 | NA | |
| 48 | hsa-miR-221-3p | HIPK2 | 1.12 | 0 | -0.24 | 0.08389 | miRNATAP | -0.21 | 0 | NA | |
| 49 | hsa-miR-221-3p | HIPK3 | 1.12 | 0 | -1.5 | 0 | MirTarget | -0.3 | 0.00068 | NA | |
| 50 | hsa-miR-221-3p | HIVEP1 | 1.12 | 0 | -1.11 | 0 | miRNAWalker2 validate | -0.21 | 0 | NA | |
| 51 | hsa-miR-221-3p | HMBOX1 | 1.12 | 0 | -0.65 | 0 | MirTarget; miRNATAP | -0.14 | 0.00024 | NA | |
| 52 | hsa-miR-221-3p | HOOK1 | 1.12 | 0 | -1.01 | 0 | MirTarget | -0.21 | 2.0E-5 | NA | |
| 53 | hsa-miR-221-3p | INSIG1 | 1.12 | 0 | -0.98 | 0.00012 | MirTarget; miRNATAP | -0.66 | 0 | NA | |
| 54 | hsa-miR-221-3p | IRF2 | 1.12 | 0 | -0.49 | 0 | miRNATAP | -0.13 | 1.0E-5 | 24607843 | Survival in patients with high risk prostate cancer is predicted by miR 221 which regulates proliferation apoptosis and invasion of prostate cancer cells by inhibiting IRF2 and SOCS3; miR-221 directly inhibits the expression of SOCS3 and IRF2 two oncogenes that negatively regulate this signaling pathway |
| 55 | hsa-miR-221-3p | ITGB3 | 1.12 | 0 | -0.98 | 0.00023 | MirTarget | -0.39 | 0 | NA | |
| 56 | hsa-miR-221-3p | KBTBD8 | 1.12 | 0 | -0.54 | 0.00302 | MirTarget | -0.2 | 0.0004 | NA | |
| 57 | hsa-miR-221-3p | KCNK2 | 1.12 | 0 | -1.05 | 0.00451 | MirTarget | -0.34 | 0.0028 | NA | |
| 58 | hsa-miR-221-3p | KIAA0430 | 1.12 | 0 | -0.22 | 0.00176 | MirTarget; miRNATAP | -0.11 | 0 | NA | |
| 59 | hsa-miR-221-3p | KLF9 | 1.12 | 0 | -1.36 | 0 | miRNAWalker2 validate | -0.56 | 0 | NA | |
| 60 | hsa-miR-221-3p | KLHL8 | 1.12 | 0 | -0.86 | 0 | miRNAWalker2 validate | -0.33 | 0 | NA | |
| 61 | hsa-miR-221-3p | KPNA6 | 1.12 | 0 | -0.22 | 0.00129 | miRNAWalker2 validate | -0.12 | 0 | NA | |
| 62 | hsa-miR-221-3p | LIFR | 1.12 | 0 | -3.55 | 0 | miRNATAP | -0.54 | 0 | NA | |
| 63 | hsa-miR-221-3p | LRP6 | 1.12 | 0 | -0.37 | 0.00135 | miRNAWalker2 validate | -0.1 | 0.0052 | NA | |
| 64 | hsa-miR-221-3p | LRRCC1 | 1.12 | 0 | 0.04 | 0.83196 | MirTarget | -0.2 | 0.00103 | NA | |
| 65 | hsa-miR-221-3p | MAP3K2 | 1.12 | 0 | -0.77 | 0 | MirTarget | -0.24 | 0 | NA | |
| 66 | hsa-miR-221-3p | MAPK10 | 1.12 | 0 | -1.09 | 3.0E-5 | miRNATAP | -0.35 | 2.0E-5 | NA | |
| 67 | hsa-miR-221-3p | MEGF9 | 1.12 | 0 | -0.77 | 0 | MirTarget; miRNATAP | -0.15 | 6.0E-5 | NA | |
| 68 | hsa-miR-221-3p | MFN2 | 1.12 | 0 | -0.6 | 0 | miRNAWalker2 validate | -0.21 | 0 | NA | |
| 69 | hsa-miR-221-3p | MIER3 | 1.12 | 0 | -0.29 | 0.00044 | MirTarget; miRNATAP | -0.11 | 1.0E-5 | NA | |
| 70 | hsa-miR-221-3p | MRAP | 1.12 | 0 | -1.12 | 0.01759 | MirTarget | -0.34 | 0.02078 | NA | |
| 71 | hsa-miR-221-3p | MYO10 | 1.12 | 0 | -1.6 | 0 | MirTarget; miRNATAP | -0.16 | 0.02642 | NA | |
| 72 | hsa-miR-221-3p | NAP1L5 | 1.12 | 0 | -1.3 | 0 | MirTarget; miRNATAP | -0.35 | 0 | NA | |
| 73 | hsa-miR-221-3p | NDFIP1 | 1.12 | 0 | -0.23 | 0.00532 | miRNAWalker2 validate | -0.15 | 0 | NA | |
| 74 | hsa-miR-221-3p | NDST3 | 1.12 | 0 | -5.21 | 0 | MirTarget | -0.51 | 0 | NA | |
| 75 | hsa-miR-221-3p | NDUFS1 | 1.12 | 0 | -0.68 | 0 | miRNAWalker2 validate | -0.21 | 0 | NA | |
| 76 | hsa-miR-221-3p | NRG1 | 1.12 | 0 | -3.96 | 0 | miRNATAP | -1.23 | 0 | NA | |
| 77 | hsa-miR-221-3p | NTF3 | 1.12 | 0 | -3.98 | 0 | miRNATAP | -0.49 | 0 | NA | |
| 78 | hsa-miR-221-3p | PAFAH1B2 | 1.12 | 0 | -0.5 | 0.00261 | MirTarget | -0.14 | 0.00473 | NA | |
| 79 | hsa-miR-221-3p | PCDHA12 | 1.12 | 0 | -2.68 | 0 | MirTarget | -0.46 | 0.00081 | NA | |
| 80 | hsa-miR-221-3p | PCDHA13 | 1.12 | 0 | -2.26 | 0 | MirTarget | -0.5 | 0.0004 | NA | |
| 81 | hsa-miR-221-3p | PCDHAC1 | 1.12 | 0 | -2.4 | 0 | MirTarget | -0.48 | 6.0E-5 | NA | |
| 82 | hsa-miR-221-3p | PCDHAC2 | 1.12 | 0 | -2.81 | 0 | MirTarget | -0.58 | 4.0E-5 | NA | |
| 83 | hsa-miR-221-3p | PDIK1L | 1.12 | 0 | -0.05 | 0.60807 | miRNAWalker2 validate | -0.11 | 0.00014 | NA | |
| 84 | hsa-miR-221-3p | PEX1 | 1.12 | 0 | -0.29 | 0.01099 | miRNAWalker2 validate | -0.14 | 6.0E-5 | NA | |
| 85 | hsa-miR-221-3p | PEX19 | 1.12 | 0 | -0.22 | 0.02448 | miRNAWalker2 validate | -0.12 | 0.00011 | NA | |
| 86 | hsa-miR-221-3p | PIK3R1 | 1.12 | 0 | -0.89 | 0 | MirTarget | -0.22 | 1.0E-5 | NA | |
| 87 | hsa-miR-221-3p | PITPNM2 | 1.12 | 0 | 0.25 | 0.07997 | MirTarget | -0.12 | 0.00652 | NA | |
| 88 | hsa-miR-221-3p | PLSCR4 | 1.12 | 0 | -2.22 | 0 | MirTarget | -0.42 | 0 | NA | |
| 89 | hsa-miR-221-3p | PPARGC1A | 1.12 | 0 | -1.91 | 0 | miRNATAP | -0.87 | 0 | NA | |
| 90 | hsa-miR-221-3p | PPM1L | 1.12 | 0 | -0.36 | 0.07741 | mirMAP | -0.35 | 0 | NA | |
| 91 | hsa-miR-221-3p | PRDM2 | 1.12 | 0 | -0.34 | 5.0E-5 | miRNATAP | -0.13 | 0 | NA | |
| 92 | hsa-miR-221-3p | RAPH1 | 1.12 | 0 | -0.83 | 0 | mirMAP | -0.25 | 0 | NA | |
| 93 | hsa-miR-221-3p | REV3L | 1.12 | 0 | -0.48 | 0.00016 | miRNATAP | -0.14 | 0.00035 | NA | |
| 94 | hsa-miR-221-3p | RNF152 | 1.12 | 0 | -2 | 0 | mirMAP | -0.53 | 0 | NA | |
| 95 | hsa-miR-221-3p | RUNDC3B | 1.12 | 0 | -1.77 | 0 | miRNAWalker2 validate | -0.64 | 0 | NA | |
| 96 | hsa-miR-221-3p | SAMD5 | 1.12 | 0 | -3.18 | 0 | mirMAP | -0.72 | 0 | NA | |
| 97 | hsa-miR-221-3p | SEC62 | 1.12 | 0 | -0.92 | 0 | miRNATAP | -0.18 | 0 | NA | |
| 98 | hsa-miR-221-3p | SELE | 1.12 | 0 | -2.35 | 0 | miRNAWalker2 validate | -0.53 | 0 | NA | |
| 99 | hsa-miR-221-3p | SEMA5A | 1.12 | 0 | -1.14 | 0 | mirMAP | -0.46 | 0 | NA | |
| 100 | hsa-miR-221-3p | SEMA6D | 1.12 | 0 | -1.74 | 0 | mirMAP; miRNATAP | -0.31 | 1.0E-5 | NA | |
| 101 | hsa-miR-221-3p | SLC4A4 | 1.12 | 0 | -2.23 | 0 | miRNATAP | -0.78 | 0 | NA | |
| 102 | hsa-miR-221-3p | SLC5A3 | 1.12 | 0 | -1.14 | 0 | mirMAP | -0.14 | 0.00448 | NA | |
| 103 | hsa-miR-221-3p | SNX4 | 1.12 | 0 | -0.54 | 0 | MirTarget | -0.15 | 0 | NA | |
| 104 | hsa-miR-221-3p | SOD2 | 1.12 | 0 | -0.69 | 0.0001 | MirTarget | -0.31 | 0 | NA | |
| 105 | hsa-miR-221-3p | STX1B | 1.12 | 0 | -0.64 | 0.00173 | miRNATAP | -0.46 | 0 | NA | |
| 106 | hsa-miR-221-3p | SUN2 | 1.12 | 0 | -0.93 | 0 | miRNATAP | -0.18 | 0 | NA | |
| 107 | hsa-miR-221-3p | SYBU | 1.12 | 0 | -1.03 | 1.0E-5 | MirTarget; miRNATAP | -0.53 | 0 | NA | |
| 108 | hsa-miR-221-3p | TAOK1 | 1.12 | 0 | -0.4 | 0.0493 | miRNATAP | -0.16 | 0.01082 | NA | |
| 109 | hsa-miR-221-3p | TIAM1 | 1.12 | 0 | -1.56 | 0 | miRNAWalker2 validate | -0.28 | 4.0E-5 | NA | |
| 110 | hsa-miR-221-3p | TIMP3 | 1.12 | 0 | -0.48 | 0.00042 | miRNAWalker2 validate; miRTarBase; MirTarget; miRNATAP | -0.14 | 0.00056 | 27077469 | MiR 221 Exacerbate Cell Proliferation and Invasion by Targeting TIMP3 in Papillary Thyroid Carcinoma; The results indicated that miR-221 exacerbated PTC by downregulating the expression of TIMP3; The effects of miR-221 and TIMP3 in vivo were also confirmed by human PTC-bearing mice models which suggest consistent results with those in vitro studies |
| 111 | hsa-miR-221-3p | TIPARP | 1.12 | 0 | -0.96 | 0 | miRNAWalker2 validate | -0.12 | 0.00104 | NA | |
| 112 | hsa-miR-221-3p | TLE3 | 1.12 | 0 | -0.22 | 0.18267 | MirTarget; miRNATAP | -0.13 | 0.01085 | NA | |
| 113 | hsa-miR-221-3p | TMED7 | 1.12 | 0 | -0.25 | 0.00735 | miRNAWalker2 validate; miRTarBase | -0.13 | 1.0E-5 | NA | |
| 114 | hsa-miR-221-3p | TMEM25 | 1.12 | 0 | -1.87 | 0 | MirTarget | -0.66 | 0 | NA | |
| 115 | hsa-miR-221-3p | TMEM56 | 1.12 | 0 | -1.76 | 0 | mirMAP | -0.48 | 0 | NA | |
| 116 | hsa-miR-221-3p | TMEM64 | 1.12 | 0 | -0 | 0.99361 | miRNAWalker2 validate | -0.12 | 0.02134 | NA | |
| 117 | hsa-miR-221-3p | TNFSF10 | 1.12 | 0 | -0.72 | 5.0E-5 | miRNAWalker2 validate; miRTarBase | -0.28 | 0 | NA | |
| 118 | hsa-miR-221-3p | TNIP1 | 1.12 | 0 | 0.05 | 0.64194 | miRNAWalker2 validate | -0.14 | 1.0E-5 | NA | |
| 119 | hsa-miR-221-3p | TSC22D2 | 1.12 | 0 | -1.12 | 0 | miRNAWalker2 validate | -0.2 | 0 | NA | |
| 120 | hsa-miR-221-3p | WNK3 | 1.12 | 0 | -0.84 | 0.00786 | miRNATAP | -0.36 | 0.0002 | NA | |
| 121 | hsa-miR-221-3p | ZADH2 | 1.12 | 0 | -1.17 | 0 | MirTarget | -0.2 | 0 | NA | |
| 122 | hsa-miR-221-3p | ZC3H13 | 1.12 | 0 | -0.68 | 0 | MirTarget | -0.14 | 0.00066 | NA | |
| 123 | hsa-miR-221-3p | ZEB2 | 1.12 | 0 | -1.51 | 0 | miRNAWalker2 validate; MirTarget; miRNATAP | -0.18 | 0.00069 | NA | |
| 124 | hsa-miR-221-3p | ZFPM2 | 1.12 | 0 | -2.22 | 0 | miRNATAP | -0.47 | 0 | NA |
| Num | GO | Overlap | Size | P Value | Adj. P Value |
|---|---|---|---|---|---|
| 1 | NEGATIVE REGULATION OF CELL DEATH | 21 | 872 | 8.92e-08 | 0.0004151 |
| 2 | REGULATION OF INTRACELLULAR SIGNAL TRANSDUCTION | 28 | 1656 | 8.049e-07 | 0.001493 |
| 3 | REGULATION OF CELL DEATH | 26 | 1472 | 9.626e-07 | 0.001493 |
| 4 | POSITIVE REGULATION OF RESPONSE TO STIMULUS | 29 | 1929 | 5.208e-06 | 0.006058 |
| 5 | POSITIVE REGULATION OF CELL COMMUNICATION | 24 | 1532 | 2.063e-05 | 0.009601 |
| 6 | POSITIVE REGULATION OF VASCULATURE DEVELOPMENT | 7 | 133 | 1.936e-05 | 0.009601 |
| 7 | POSITIVE REGULATION OF RESPONSE TO EXTERNAL STIMULUS | 10 | 296 | 1.6e-05 | 0.009601 |
| 8 | POSITIVE REGULATION OF MULTICELLULAR ORGANISMAL PROCESS | 23 | 1395 | 1.406e-05 | 0.009601 |
| 9 | REGULATION OF ANATOMICAL STRUCTURE MORPHOGENESIS | 19 | 1021 | 1.688e-05 | 0.009601 |
| 10 | REGULATION OF PHOSPHORUS METABOLIC PROCESS | 25 | 1618 | 1.696e-05 | 0.009601 |
| Num | GO | Overlap | Size | P Value | Adj. P Value |
|---|
| Num | GO | Overlap | Size | P Value | Adj. P Value |
|---|
Over-represented Pathway
| Num | Pathway | Pathview | Overlap | Size | P Value | Adj. P Value |
|---|---|---|---|---|---|---|
| 1 | FoxO_signaling_pathway_hsa04068 | 6 | 132 | 0.0001742 | 0.004848 | |
| 2 | ErbB_signaling_pathway_hsa04012 | 5 | 85 | 0.0001865 | 0.004848 | |
| 3 | Ras_signaling_pathway_hsa04014 | 7 | 232 | 0.0006159 | 0.009251 | |
| 4 | Mitophagy_animal_hsa04137 | 4 | 65 | 0.0007116 | 0.009251 | |
| 5 | Cellular_senescence_hsa04218 | 5 | 160 | 0.003227 | 0.03357 | |
| 6 | TNF_signaling_pathway_hsa04668 | 4 | 108 | 0.004593 | 0.03981 | |
| 7 | Apoptosis_hsa04210 | 4 | 138 | 0.01076 | 0.07994 | |
| 8 | Peroxisome_hsa04146 | 3 | 83 | 0.01491 | 0.09694 | |
| 9 | cAMP_signaling_pathway_hsa04024 | 4 | 198 | 0.03492 | 0.1916 | |
| 10 | MAPK_signaling_pathway_hsa04010 | 5 | 295 | 0.03684 | 0.1916 | |
| 11 | Autophagy_animal_hsa04140 | 3 | 128 | 0.04542 | 0.2147 | |
| 12 | Phospholipase_D_signaling_pathway_hsa04072 | 3 | 146 | 0.06248 | 0.2707 | |
| 13 | mTOR_signaling_pathway_hsa04150 | 3 | 151 | 0.06768 | 0.2707 | |
| 14 | Tight_junction_hsa04530 | 3 | 170 | 0.08914 | 0.3311 | |
| 15 | Gap_junction_hsa04540 | 2 | 88 | 0.1036 | 0.359 | |
| 16 | Phosphatidylinositol_signaling_system_hsa04070 | 2 | 99 | 0.1257 | 0.3617 | |
| 17 | Focal_adhesion_hsa04510 | 3 | 199 | 0.1265 | 0.3617 | |
| 18 | HIF_1_signaling_pathway_hsa04066 | 2 | 100 | 0.1278 | 0.3617 | |
| 19 | Rap1_signaling_pathway_hsa04015 | 3 | 206 | 0.1363 | 0.3617 | |
| 20 | Regulation_of_actin_cytoskeleton_hsa04810 | 3 | 208 | 0.1391 | 0.3617 | |
| 21 | Sphingolipid_signaling_pathway_hsa04071 | 2 | 118 | 0.1663 | 0.3962 | |
| 22 | AMPK_signaling_pathway_hsa04152 | 2 | 121 | 0.1729 | 0.3962 | |
| 23 | PI3K_Akt_signaling_pathway_hsa04151 | 4 | 352 | 0.1753 | 0.3962 | |
| 24 | Signaling_pathways_regulating_pluripotency_of_stem_cells_hsa04550 | 2 | 139 | 0.2132 | 0.4365 | |
| 25 | Cell_adhesion_molecules_.CAMs._hsa04514 | 2 | 145 | 0.2269 | 0.4365 | |
| 26 | Wnt_signaling_pathway_hsa04310 | 2 | 146 | 0.2292 | 0.4365 | |
| 27 | Cytokine_cytokine_receptor_interaction_hsa04060 | 3 | 270 | 0.2351 | 0.4365 | |
| 28 | Jak_STAT_signaling_pathway_hsa04630 | 2 | 162 | 0.2659 | 0.4688 | |
| 29 | Necroptosis_hsa04217 | 2 | 164 | 0.2705 | 0.4688 | |
| 30 | Endocytosis_hsa04144 | 2 | 244 | 0.448 | 0.6852 |
