This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are
presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.
"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.
Num | microRNA | Gene | miRNA log2FC | miRNA pvalue | Gene log2FC | Gene pvalue | Interaction | Correlation beta | Correlation P-value | PMID | Reported in cancer studies |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | hsa-miR-195-5p | OTUB1 | -1.45 | 0 | 0.64 | 0 | miRNATAP | -0.13 | 0 | NA |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | PROTEIN MODIFICATION BY SMALL PROTEIN CONJUGATION OR REMOVAL | 14 | 873 | 1.862e-11 | 6.615e-08 |
2 | PROTEIN MODIFICATION BY SMALL PROTEIN REMOVAL | 8 | 133 | 2.844e-11 | 6.615e-08 |
3 | CELLULAR RESPONSE TO DNA DAMAGE STIMULUS | 12 | 720 | 5.04e-10 | 7.817e-07 |
4 | PROTEIN CATABOLIC PROCESS | 10 | 579 | 1.39e-08 | 1.617e-05 |
5 | DNA REPAIR | 9 | 480 | 4.209e-08 | 3.264e-05 |
6 | CELLULAR RESPONSE TO STRESS | 14 | 1565 | 3.739e-08 | 3.264e-05 |
7 | NON RECOMBINATIONAL REPAIR | 5 | 70 | 8.499e-08 | 5.649e-05 |
8 | MACROMOLECULE CATABOLIC PROCESS | 11 | 926 | 1.041e-07 | 6.055e-05 |
9 | DOUBLE STRAND BREAK REPAIR | 6 | 165 | 2.184e-07 | 0.0001129 |
10 | CATABOLIC PROCESS | 13 | 1773 | 1.387e-06 | 0.0006265 |
11 | PROTEOLYSIS | 11 | 1208 | 1.481e-06 | 0.0006265 |
12 | DNA METABOLIC PROCESS | 9 | 758 | 1.969e-06 | 0.0007637 |
13 | REGULATION OF RESPONSE TO DNA DAMAGE STIMULUS | 5 | 145 | 3.215e-06 | 0.001151 |
14 | CELLULAR CATABOLIC PROCESS | 11 | 1322 | 3.572e-06 | 0.001182 |
15 | CELL CYCLE ARREST | 5 | 154 | 4.318e-06 | 0.001182 |
16 | PROTEIN K48 LINKED DEUBIQUITINATION | 3 | 20 | 4.164e-06 | 0.001182 |
17 | ESTABLISHMENT OF PROTEIN LOCALIZATION TO GOLGI | 3 | 20 | 4.164e-06 | 0.001182 |
18 | REGULATION OF DNA REPAIR | 4 | 75 | 6.057e-06 | 0.001566 |
19 | PROTEIN K63 LINKED DEUBIQUITINATION | 3 | 24 | 7.36e-06 | 0.001712 |
20 | RETROGRADE TRANSPORT VESICLE RECYCLING WITHIN GOLGI | 3 | 24 | 7.36e-06 | 0.001712 |
21 | REGULATION OF CELLULAR RESPONSE TO STRESS | 8 | 691 | 9.827e-06 | 0.002177 |
22 | REGULATION OF DNA METABOLIC PROCESS | 6 | 340 | 1.441e-05 | 0.003048 |
23 | SOMATIC CELL DNA RECOMBINATION | 3 | 33 | 1.965e-05 | 0.003657 |
24 | SOMATIC DIVERSIFICATION OF IMMUNE RECEPTORS VIA GERMLINE RECOMBINATION WITHIN A SINGLE LOCUS | 3 | 33 | 1.965e-05 | 0.003657 |
25 | PROTEIN LOCALIZATION TO GOLGI APPARATUS | 3 | 33 | 1.965e-05 | 0.003657 |
26 | MULTICELLULAR ORGANISM REPRODUCTION | 8 | 768 | 2.112e-05 | 0.00378 |
27 | NEGATIVE REGULATION OF DNA METABOLIC PROCESS | 4 | 111 | 2.866e-05 | 0.00494 |
28 | GAMETE GENERATION | 7 | 595 | 3.506e-05 | 0.005827 |
29 | SOMATIC DIVERSIFICATION OF IMMUNE RECEPTORS | 3 | 42 | 4.094e-05 | 0.006568 |
30 | INTRA GOLGI VESICLE MEDIATED TRANSPORT | 3 | 47 | 5.751e-05 | 0.008632 |
31 | NEGATIVE REGULATION OF CELL CYCLE | 6 | 433 | 5.591e-05 | 0.008632 |
32 | MULTI ORGANISM REPRODUCTIVE PROCESS | 8 | 891 | 6.09e-05 | 0.008855 |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|
1 | THIOL DEPENDENT UBIQUITIN SPECIFIC PROTEASE ACTIVITY | 8 | 73 | 2.08e-13 | 1.933e-10 |
2 | CYSTEINE TYPE PEPTIDASE ACTIVITY | 9 | 184 | 9.068e-12 | 3.118e-09 |
3 | UBIQUITIN LIKE PROTEIN SPECIFIC PROTEASE ACTIVITY | 8 | 117 | 1.007e-11 | 3.118e-09 |
4 | UBIQUITIN LIKE PROTEIN BINDING | 7 | 121 | 7.393e-10 | 1.717e-07 |
5 | PEPTIDASE ACTIVITY | 9 | 663 | 6.483e-07 | 0.0001204 |
6 | K63 LINKED POLYUBIQUITIN BINDING | 3 | 19 | 3.543e-06 | 0.0005486 |
7 | ZINC ION BINDING | 10 | 1155 | 7.955e-06 | 0.001056 |
8 | POLYUBIQUITIN BINDING | 3 | 42 | 4.094e-05 | 0.004378 |
9 | TRANSITION METAL ION BINDING | 10 | 1400 | 4.241e-05 | 0.004378 |
Num | GO | Overlap | Size | P Value | Adj. P Value |
---|
Num | Pathway | Pathview | Overlap | Size | P Value | Adj. P Value |
---|---|---|---|---|---|---|
1 | Cell_cycle_hsa04110 | 2 | 124 | 0.01675 | 0.213 | |
2 | Autophagy_animal_hsa04140 | 2 | 128 | 0.01778 | 0.213 | |
3 | FoxO_signaling_pathway_hsa04068 | 2 | 132 | 0.01884 | 0.213 | |
4 | Apelin_signaling_pathway_hsa04371 | 2 | 137 | 0.0202 | 0.213 | |
5 | Apoptosis_hsa04210 | 2 | 138 | 0.02048 | 0.213 | |
6 | Cellular_senescence_hsa04218 | 2 | 160 | 0.02696 | 0.2337 |