miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-105-5p AKT2 -0.567459149851677 8.20858944634795e-06 0.139361896216647 0.0151763675439579 mirMAP -0.11517777162873 2.5456118337521e-14 NA NA NA hsa-miR-146b-5p AKT3 0.441078059667297 0.000237619929889385 -0.235109681312394 0.000861339724103604 miRNAWalker2_validate -0.14853390000962 1.54850741316524e-10 NA NA NA hsa-miR-15a-5p AKT3 0.142658985373611 0.0536219185009004 -0.235109681312394 0.000861339724103604 miRNAWalker2_validate;miRTarBase;miRNATAP -0.171093255137198 0.000104922528677221 NA NA NA hsa-miR-15b-5p AKT3 0.592863156692837 1.41971517354055e-10 -0.235109681312394 0.000861339724103604 miRNATAP -0.149023414077877 1.00397425898265e-06 NA NA NA hsa-miR-28-3p AKT3 0.139444423971018 0.0811187936164314 -0.235109681312394 0.000861339724103604 miRNATAP -0.281326919526608 6.94030005162363e-11 NA NA NA hsa-miR-320a AKT3 0.092135599636725 0.243028758989929 -0.235109681312394 0.000861339724103604 PITA;miRanda;miRNATAP -0.162091233103736 0.000150010109497495 NA NA NA hsa-miR-320c AKT3 0.343259124189966 0.000248338401291051 -0.235109681312394 0.000861339724103604 PITA;miRanda;miRNATAP -0.11345587888064 0.000121683825333423 NA NA NA hsa-miR-362-3p AKT3 0.032375035235532 0.736465332563774 -0.235109681312394 0.000861339724103604 miRanda -0.133145359057788 1.93504628679194e-06 NA NA NA hsa-miR-362-5p AKT3 -0.0730185557229279 0.425572997292224 -0.235109681312394 0.000861339724103604 PITA;TargetScan;miRNATAP -0.12269062278053 4.12753291036148e-05 NA NA NA hsa-miR-501-3p AKT3 0.0726267165568464 0.382729320534971 -0.235109681312394 0.000861339724103604 miRNATAP -0.230986821283872 3.52473505159126e-12 NA NA NA hsa-miR-502-3p AKT3 0.0276791089364088 0.697605011368848 -0.235109681312394 0.000861339724103604 miRNATAP -0.217874003496912 1.14360007951059e-08 NA NA NA hsa-miR-93-5p AKT3 0.139766237971122 0.101057320076629 -0.235109681312394 0.000861339724103604 miRNATAP -0.208262975522215 1.91431764461584e-05 NA NA NA hsa-miR-125a-5p ANGPT2 -0.0833349424517387 0.325440200253607 0.973073130335985 3.11405321255146e-13 miRanda -0.264795607577102 0.00227167877390085 NA NA NA hsa-miR-129-5p ANGPT2 -0.567133990117126 0.00116152215679936 0.973073130335985 3.11405321255146e-13 MirTarget;miRanda -0.318009570105921 2.19601545367872e-21 NA NA NA hsa-miR-135a-5p ANGPT2 -0.122734612943241 0.196985964641886 0.973073130335985 3.11405321255146e-13 MirTarget -0.302595816265356 2.66606536108577e-06 NA NA NA hsa-miR-138-5p ANGPT2 -0.616087649920851 1.35200027885729e-05 0.973073130335985 3.11405321255146e-13 MirTarget -0.292625041668636 1.45807993019987e-12 NA NA NA hsa-miR-299-5p ANGPT2 -0.0814737109317336 0.57620244226785 0.973073130335985 3.11405321255146e-13 miRNATAP -0.129772479402096 0.00169668460048174 NA NA NA hsa-miR-381-3p ANGPT2 -0.192118086401438 0.203198554750071 0.973073130335985 3.11405321255146e-13 mirMAP -0.136683239389105 0.000582223002645419 NA NA NA hsa-miR-491-3p ANGPT2 -0.523374665885358 1.93479565404951e-05 0.973073130335985 3.11405321255146e-13 MirTarget;miRNATAP -0.358806940804183 3.01453423361413e-14 NA NA NA hsa-miR-539-5p ANGPT2 -0.326485413953598 0.0554453076411246 0.973073130335985 3.11405321255146e-13 mirMAP -0.281490575938618 8.3747590779385e-17 NA NA NA hsa-miR-543 ANGPT2 -0.347737549779625 0.0157394585079683 0.973073130335985 3.11405321255146e-13 MirTarget;PITA;miRanda;miRNATAP -0.240688906068078 8.16359448069276e-09 NA NA NA hsa-miR-582-5p ANGPT2 -0.215764335500665 0.0760892640434509 0.973073130335985 3.11405321255146e-13 mirMAP -0.229736619223816 1.95985705135758e-06 NA NA NA hsa-miR-744-3p ANGPT2 -0.226053568116656 0.0326553280112967 0.973073130335985 3.11405321255146e-13 MirTarget -0.268865282847753 2.38316775512343e-06 NA NA NA hsa-miR-9-5p ANGPT2 -0.125377971246422 0.269896477802284 0.973073130335985 3.11405321255146e-13 MirTarget -0.602205129123588 3.59342523707757e-06 NA NA NA hsa-miR-15a-5p ATF2 0.142658985373611 0.0536219185009004 -0.0726138691955862 0.33392541656757 miRNAWalker2_validate -0.144652702403681 0.0041247080050325 NA NA NA hsa-miR-19a-3p ATF2 0.0466707843731911 0.576818938277755 -0.0726138691955862 0.33392541656757 MirTarget;miRNATAP -0.127370853754012 0.000958008133873 NA NA NA hsa-miR-19b-3p ATF2 -0.0138427450847924 0.856506310673067 -0.0726138691955862 0.33392541656757 miRNAWalker2_validate;MirTarget;miRNATAP -0.172670973704451 0.000168434540469046 NA NA NA hsa-miR-26b-5p ATF2 -0.0984536538762768 0.231803769574623 -0.0726138691955862 0.33392541656757 MirTarget;miRNATAP -0.164082559964853 0.000243149817199337 21901137 Coordinated regulation of ATF2 by miR 26b in γ irradiated lung cancer cells; Concurrent analysis of time-series mRNA and microRNA profiles uncovered that expression of miR-26b was down regulated and its target activating transcription factor 2 ATF2 mRNA was up regulated in γ-irradiated H1299 cells; IR in miR-26b overexpressed H1299 cells could not induce expression of ATF2; From these results we concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells which may enhance the effect of IR in the MAPK signaling pathway lung cancer hsa-miR-30a-5p ATF2 -0.062942936611238 0.513851954700252 -0.0726138691955862 0.33392541656757 mirMAP -0.16690467064425 0.00156810646595739 NA NA NA hsa-miR-30b-5p ATF2 -0.0858101016322106 0.263243418521854 -0.0726138691955862 0.33392541656757 mirMAP -0.188763555453189 9.29345111461018e-05 NA NA NA hsa-miR-30c-5p ATF2 -0.0469114469943097 0.565934557464627 -0.0726138691955862 0.33392541656757 mirMAP -0.262852827602014 4.81664922337019e-08 NA NA NA hsa-miR-320a ATF2 0.092135599636725 0.243028758989929 -0.0726138691955862 0.33392541656757 miRanda -0.135329118151361 0.00562986282916445 NA NA NA hsa-miR-342-3p ATF6B -0.125683829770877 0.105810751640137 0.0390929867572121 0.385442480159985 miRanda -0.10557428229826 2.50869393945283e-10 NA NA NA hsa-miR-15a-5p BCL2 0.142658985373611 0.0536219185009004 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.15185736460016 0.000524610161257898 25594541;26915294;18931683;25623762;22335947 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MicroRNAs miRNAs are noncoding small RNAs that repress protein translation by targeting specific messenger RNAs miR-15a and miR-16-1 act as putative tumor suppressors by targeting the oncogene BCL2;miR 15a and miR 16 modulate drug resistance by targeting bcl 2 in human colon cancer cells; To investigate the reversal effect of targeted modulation of bcl-2 expression by miR-15a and miR-16 on drug resistance of human colon cancer cells;The expression of MiR-15a was significantly inhibited by Bcl-2 P < 0.05 ;poor survival;;drug resistance; breast cancer;lung cancer;prostate cancer;colon cancer;breast cancer hsa-miR-15b-5p BCL2 0.592863156692837 1.41971517354055e-10 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.15277272102586 4.15473292044312e-07 25594541;26915294;26884837;18449891 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MiR 15b mediates liver cancer cells proliferation through targeting BCL 2; MiR-15b overexpression downregulated BCL2 mRNA and protein expression obviously P < 0.05; On the contrary miR-15b inhibitor transfection markedly reduced miR-15b expression in liver cancer cells P < 0.05 promoted tumor cell proliferation and increased BCL2 mRNA and protein expression; MiR-15b can inhibit HepG2 cell proliferation and down-regulate BCL2 mRNA and protein expression;miR 15b and miR 16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells; The downregulation of miR-15b and miR-16 in SGC7901/VCR cells was concurrent with the upregulation of Bcl-2 protein; Taken together our findings suggest that miR-15b and miR-16 could play a role in the development of MDR in gastric cancer cells at least in part by modulation of apoptosis via targeting BCL2 ;poor survival;;drug resistance breast cancer;lung cancer;liver cancer;gastric cancer hsa-miR-17-5p BCL2 0.0975751369167082 0.20621110214601 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.139765906763986 0.00117394398758695 25435430 Combined overexpression of miR-16 and miR-17 greatly reduced Beclin-1 and Bcl-2 expressions respectively; miR-17 overexpression reduced cytoprotective autophagy by targeting Beclin-1 whereas overexpression of miR-16 potentiated paclitaxel induced apoptotic cell death by inhibiting anti-apoptotic protein Bcl-2 lung cancer hsa-miR-20a-5p BCL2 -0.0115865752288853 0.887069448776018 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.191068358926279 1.1049320794207e-06 NA NA NA hsa-miR-140-5p BCL2L11 0.065063507103468 0.380452509211773 -0.0976090569425967 0.162377907156097 miRanda -0.108751841465347 0.00927302890339163 NA NA NA hsa-miR-181b-5p BCL2L11 0.0260899284211646 0.768941836912518 -0.0976090569425967 0.162377907156097 miRNATAP -0.115801022312088 0.00394903405108419 26572075 MiR 181b promotes chemoresistance in breast cancer by regulating Bim expression; Mechanistic studies demonstrated that the Bim gene an essential initiator of apoptosis was inhibited by miR-181b overexpression; Importantly we demonstrated that miR-181b inhibitors increased the level of Bim in the T-47D-R cells resulting in the loss of mitochondrial membrane potential MMP and the activation of caspases caused by DOX drug resistance breast cancer hsa-miR-30b-5p BCL2L11 -0.0858101016322106 0.263243418521854 -0.0976090569425967 0.162377907156097 miRNATAP -0.153204497023672 0.000625463211295477 NA NA NA hsa-miR-30d-5p BCL2L11 -0.0607010868720046 0.471410043548794 -0.0976090569425967 0.162377907156097 miRNATAP -0.136615268044834 0.00784838233049484 NA NA NA hsa-miR-7-1-3p BCL2L11 -0.0894475316162393 0.37322359785108 -0.0976090569425967 0.162377907156097 mirMAP -0.130415036593749 4.51023194161572e-06 NA NA NA hsa-miR-3065-3p BDNF -0.261348288537884 0.0343753235107924 0.163817385359977 0.296772674802745 MirTarget;miRNATAP -0.263514713378115 5.47125066901657e-06 NA NA NA hsa-miR-3065-5p BDNF -0.262608140481507 0.0414570974148034 0.163817385359977 0.296772674802745 mirMAP;miRNATAP -0.276290459467715 1.96100180067284e-07 NA NA NA hsa-miR-181a-5p BRCA1 -0.178215604431845 0.0700010802255601 0.539440908142048 1.91664734408029e-16 miRNAWalker2_validate -0.109895198869886 0.00587176220728963 NA NA NA hsa-miR-342-3p BRCA1 -0.125683829770877 0.105810751640137 0.539440908142048 1.91664734408029e-16 miRanda -0.126491796147093 0.00219805679571227 26919240;24475217 miR 342 overexpression results in a synthetic lethal phenotype in BRCA1 mutant HCC1937 breast cancer cells; We investigated the effects of miR-342 transfection in the triple-negative breast cancer cell lines MDA-MB-231 and HCC1937 the latter carrying a germ-line BRCA1 mutation; Reconstitution of miR-342 led to caspase-dependent induction of apoptosis only in HCC1937 cells while overexpression of wild-type BRCA1 in HCC1937 cells counteracted miR-342-mediated induction of apoptosis suggesting that miR-342 overexpression and the lack of functional BRCA1 result in a synthetic lethal phenotype; Moreover siRNA-mediated depletion of BRCA1 in MDA-MB-231 cells expressing the wild-type protein led to apoptosis upon transfection with miR-342;miR 342 regulates BRCA1 expression through modulation of ID4 in breast cancer; Based on these findings we hypothesized that regulation of ID4 mediated by miR-342 could be involved in the pathogenesis of breast cancer by downregulating BRCA1 expression; Overexpression of miR-342 in these cells reduced ID4 and increased BRCA1 expression supporting a possible role of this mechanism in breast cancer; In the cohort of patients we studied a correlation between miR-342 and BRCA1 expression was found in the ER-negative cases ; breast cancer;breast cancer hsa-miR-125a-3p CASP9 0.183834985432376 0.106230647107917 -0.190390818122219 0.00860629060432951 miRanda -0.10922116203645 1.51947097140883e-05 NA NA NA hsa-miR-126-5p CASP9 0.177332403948664 0.0711675116243123 -0.190390818122219 0.00860629060432951 MirTarget -0.260533886267505 9.08731800375383e-19 NA NA NA hsa-miR-133a-3p CASP9 -0.335058908302964 0.0180402783121034 -0.190390818122219 0.00860629060432951 miRNAWalker2_validate;miRTarBase -0.126150713979561 1.5336707780324e-10 NA NA NA hsa-miR-140-5p CASP9 0.065063507103468 0.380452509211773 -0.190390818122219 0.00860629060432951 miRanda -0.198208812177151 1.81618262797927e-06 NA NA NA hsa-miR-15a-5p CASP9 0.142658985373611 0.0536219185009004 -0.190390818122219 0.00860629060432951 mirMAP -0.235687914842974 3.68148704667338e-07 NA NA NA hsa-miR-15b-5p CASP9 0.592863156692837 1.41971517354055e-10 -0.190390818122219 0.00860629060432951 mirMAP -0.194862381425862 1.11164072592137e-09 NA NA NA hsa-miR-16-5p CASP9 0.246024668671282 0.00346042804503716 -0.190390818122219 0.00860629060432951 mirMAP -0.203519004171527 3.05123518551225e-07 NA NA NA hsa-miR-193b-3p CASP9 0.0766333116224072 0.324723824453143 -0.190390818122219 0.00860629060432951 miRNAWalker2_validate -0.287691751817182 2.55461285152645e-14 NA NA NA hsa-miR-199a-3p CASP9 0.41226331871076 0.000497136154474528 -0.190390818122219 0.00860629060432951 mirMAP -0.203767948496481 7.25854614561419e-18 23319430 The techniques used were the MTT assay flow cytometry real-time PCR to assess miR-199a expression as also caspase-8 and caspase-9 activity in HepG2 cells treated with Propofol liver cancer hsa-miR-199b-3p CASP9 0.411848361704393 0.000517815011892379 -0.190390818122219 0.00860629060432951 mirMAP -0.202937922848071 8.40130462827375e-18 NA NA NA hsa-miR-342-5p CASP9 0.0383784541944436 0.544128449296127 -0.190390818122219 0.00860629060432951 MirTarget -0.172780651339529 0.000183272409998028 NA NA NA hsa-miR-424-5p CASP9 0.25140374049676 0.00709934939774983 -0.190390818122219 0.00860629060432951 mirMAP -0.121649518123937 0.000136932797700071 NA NA NA hsa-miR-450b-5p CASP9 0.270544879013992 0.00801537838499562 -0.190390818122219 0.00860629060432951 mirMAP -0.107405523905285 0.000148848993244956 NA NA NA hsa-miR-495-3p CASP9 -0.421982588962504 0.00504424038526901 -0.190390818122219 0.00860629060432951 mirMAP -0.112698137537794 1.81004622187323e-09 NA NA NA hsa-miR-548b-3p CASP9 0.340474544210108 0.00224491592019745 -0.190390818122219 0.00860629060432951 MirTarget -0.12601735851912 9.6237618924232e-07 NA NA NA hsa-miR-590-3p CASP9 0.312951515438396 0.000480455423843996 -0.190390818122219 0.00860629060432951 miRanda -0.109539366255286 0.000563304082980271 NA NA NA hsa-let-7e-5p CCND1 0.0471189209570344 0.657255455444655 0.517304800763483 1.04046594178888e-06 miRTarBase;miRNATAP -0.228941875441154 1.78800254985128e-06 NA NA NA hsa-let-7i-5p CCND1 0.0952853587923244 0.192120688957249 0.517304800763483 1.04046594178888e-06 miRNATAP -0.236343082962889 0.00183350614645 NA NA NA hsa-miR-1266-5p CCND1 0.313515127917259 0.0138664194727392 0.517304800763483 1.04046594178888e-06 MirTarget -0.112027246009442 0.00108554865163972 NA NA NA hsa-miR-135a-5p CCND1 -0.122734612943241 0.196985964641886 0.517304800763483 1.04046594178888e-06 mirMAP -0.247492714890572 1.3238406350361e-07 NA NA NA hsa-miR-23b-3p CCND1 0.0476610245860094 0.630277683372344 0.517304800763483 1.04046594178888e-06 miRNATAP -0.279243855267394 9.57342364736838e-09 NA NA NA hsa-miR-26b-5p CCND1 -0.0984536538762768 0.231803769574623 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate -0.171067179820866 0.00692173397836684 NA NA NA hsa-miR-29a-3p CCND1 -0.224140851576083 0.0349516103550236 0.517304800763483 1.04046594178888e-06 mirMAP -0.23358540897967 1.12603356998685e-05 NA NA NA hsa-miR-29b-3p CCND1 -0.178522712044497 0.0856106432165465 0.517304800763483 1.04046594178888e-06 mirMAP -0.30608004962895 3.25721913642118e-11 NA NA NA hsa-miR-29c-3p CCND1 -0.372180390387259 0.00202634431973208 0.517304800763483 1.04046594178888e-06 mirMAP -0.263497298429692 1.34805822955252e-11 NA NA NA hsa-miR-3065-5p CCND1 -0.262608140481507 0.0414570974148034 0.517304800763483 1.04046594178888e-06 mirMAP -0.105747126080182 0.00178155205723976 NA NA NA hsa-miR-330-3p CCND1 -0.427388106938151 0.00300489284414266 0.517304800763483 1.04046594178888e-06 mirMAP -0.156757729272097 1.33987981854083e-07 NA NA NA hsa-miR-338-3p CCND1 -0.39963704679384 0.0034410643220691 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase;miRanda -0.138060683824931 7.24806702911648e-05 NA NA NA hsa-miR-34a-5p CCND1 0.143472919835524 0.211283228567057 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.176667405034615 4.78965946188406e-06 25792709;21399894 This inhibition of proliferation was associated with a decrease in cyclin D1 levels orchestrated principally by HNF-4α a target of miR-34a considered to act as a tumour suppressor in the liver;Quantitative PCR and western analysis confirmed decreased expression of two genes BCL-2 and CCND1 in docetaxel-resistant cells which are both targeted by miR-34a ; liver cancer;breast cancer hsa-miR-365a-3p CCND1 -0.184222766207919 0.0292663235724171 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase -0.195991913128339 0.000198680829195249 NA NA NA hsa-miR-425-5p CCND1 -0.0412837711174561 0.682284485592858 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate -0.134644465621285 0.00325561137510874 NA NA NA hsa-miR-490-3p CCND1 -0.834268051506884 5.26203010743215e-06 0.517304800763483 1.04046594178888e-06 miRanda -0.131117175699242 2.90415525218072e-08 24440705 MicroRNA 490 3p inhibits proliferation of A549 lung cancer cells by targeting CCND1; We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1 which plays a key role in G1/S phase transition; In addition the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3'UTR lung cancer hsa-miR-495-3p CCND1 -0.421982588962504 0.00504424038526901 0.517304800763483 1.04046594178888e-06 MirTarget -0.136090131725584 2.1911780731285e-06 NA NA NA hsa-miR-7-1-3p CCND1 -0.0894475316162393 0.37322359785108 0.517304800763483 1.04046594178888e-06 mirMAP -0.241924898170475 2.08913227927897e-08 NA NA NA hsa-miR-769-3p CCND1 -0.287263649023213 0.018145224330845 0.517304800763483 1.04046594178888e-06 mirMAP -0.276564457533319 1.97356475814615e-15 NA NA NA hsa-miR-885-5p CCND1 -0.423614695846868 1.14213289528402e-05 0.517304800763483 1.04046594178888e-06 miRNATAP -0.292419966156513 1.08197956283879e-10 NA NA NA hsa-let-7b-3p CCND2 -0.00495618733298731 0.952288980686967 0.278072058804804 0.00230941164668614 mirMAP -0.124812140552693 0.00599796857804289 NA NA NA hsa-let-7f-1-3p CCND2 0.110713086694053 0.21484524300518 0.278072058804804 0.00230941164668614 mirMAP -0.122537465829283 0.00233902672067921 NA NA NA hsa-let-7g-5p CCND2 -0.0253396334608382 0.742556489443668 0.278072058804804 0.00230941164668614 miRNATAP -0.196434601966773 0.0012996932102575 NA NA NA hsa-let-7i-5p CCND2 0.0952853587923244 0.192120688957249 0.278072058804804 0.00230941164668614 miRNATAP -0.162156277541767 0.00988534673137717 NA NA NA hsa-miR-129-5p CCND2 -0.567133990117126 0.00116152215679936 0.278072058804804 0.00230941164668614 mirMAP -0.105272286982926 4.44505393807218e-07 NA NA NA hsa-miR-145-3p CCND2 0.110543172922298 0.294150620210019 0.278072058804804 0.00230941164668614 mirMAP -0.149944712694934 9.82587587919985e-06 NA NA NA hsa-miR-145-5p CCND2 0.108948399943015 0.310032352776693 0.278072058804804 0.00230941164668614 miRNATAP -0.155654659327631 1.04593167784463e-05 NA NA NA hsa-miR-146b-3p CCND2 0.283486177568242 0.0118918188864879 0.278072058804804 0.00230941164668614 MirTarget;PITA;miRNATAP -0.117766891479947 0.000283548163350027 NA NA NA hsa-miR-150-5p CCND2 0.0645271721519611 0.513295658828718 0.278072058804804 0.00230941164668614 mirMAP -0.149451068560082 7.57763104304709e-05 NA NA NA hsa-miR-151a-3p CCND2 0.0336074122085215 0.726834677736803 0.278072058804804 0.00230941164668614 mirMAP -0.12420907434582 0.00497911559673839 NA NA NA hsa-miR-185-5p CCND2 -0.0767432095218297 0.404113540276816 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.183398276514872 8.3630357382723e-06 NA NA NA hsa-miR-186-5p CCND2 0.0606381894130505 0.514217717592464 0.278072058804804 0.00230941164668614 mirMAP;miRNATAP -0.133720175872038 0.00173369326890833 NA NA NA hsa-miR-191-5p CCND2 0.0973986771912738 0.220078671774056 0.278072058804804 0.00230941164668614 MirTarget -0.20451499794437 0.00023723661594718 NA NA NA hsa-miR-26a-5p CCND2 0.033823776747294 0.726610414342369 0.278072058804804 0.00230941164668614 miRNAWalker2_validate;miRTarBase;mirMAP;miRNATAP -0.122521188171157 0.00669669430395561 NA NA NA hsa-miR-26b-5p CCND2 -0.0984536538762768 0.231803769574623 0.278072058804804 0.00230941164668614 mirMAP;miRNATAP -0.21765809461179 3.01296451472833e-05 NA NA NA hsa-miR-28-5p CCND2 0.104003572105781 0.153594705579534 0.278072058804804 0.00230941164668614 miRanda -0.151040812510917 0.00458703218164268 NA NA NA hsa-miR-29a-3p CCND2 -0.224140851576083 0.0349516103550236 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.199711571525784 5.61968185759315e-06 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29a-5p CCND2 -0.0697727833812678 0.350621682973119 0.278072058804804 0.00230941164668614 mirMAP -0.166759307844904 0.000688470083956136 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29b-3p CCND2 -0.178522712044497 0.0856106432165465 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.202303651104096 1.45292987304036e-07 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29c-3p CCND2 -0.372180390387259 0.00202634431973208 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.239386359038839 8.64180673949139e-14 NA NA NA hsa-miR-30d-3p CCND2 0.0854049347792898 0.20691254801121 0.278072058804804 0.00230941164668614 mirMAP -0.155355754639963 0.00382748112364014 NA NA NA hsa-miR-30e-3p CCND2 0.00173769765874887 0.984965501547409 0.278072058804804 0.00230941164668614 mirMAP -0.268522462794922 4.02496537819957e-07 NA NA NA hsa-miR-324-3p CCND2 -0.0103367134443655 0.904494071318044 0.278072058804804 0.00230941164668614 miRNAWalker2_validate -0.184828101121747 1.93642919504512e-05 NA NA NA hsa-miR-331-5p CCND2 0.00538076980695534 0.942833685963252 0.278072058804804 0.00230941164668614 miRNATAP -0.168360380366874 0.000470859110297375 NA NA NA hsa-miR-33a-3p CCND2 -0.158366376619191 0.0991512910387226 0.278072058804804 0.00230941164668614 MirTarget -0.10197096016646 0.00681801603426165 NA NA NA hsa-miR-3622a-3p CCND2 0.0389203848593471 0.75605812962459 0.278072058804804 0.00230941164668614 mirMAP -0.125163354365051 1.78891207109425e-05 NA NA NA hsa-miR-488-3p CCND2 -0.257955761012568 0.00280795162806256 0.278072058804804 0.00230941164668614 mirMAP -0.183392273105331 1.52514393662224e-05 NA NA NA hsa-miR-488-5p CCND2 -0.385755602369049 7.99431673275965e-08 0.278072058804804 0.00230941164668614 mirMAP -0.153852506035559 0.00315722783501446 NA NA NA hsa-miR-500a-5p CCND2 0.0634452729978863 0.396072382601677 0.278072058804804 0.00230941164668614 mirMAP -0.155381019814906 0.0013241335866013 NA NA NA hsa-miR-501-5p CCND2 0.0878951859817452 0.309663377222443 0.278072058804804 0.00230941164668614 PITA;mirMAP;miRNATAP -0.110941849752897 0.00801840144207187 NA NA NA hsa-miR-660-5p CCND2 -0.138989893523975 0.0945491063873448 0.278072058804804 0.00230941164668614 mirMAP -0.238701834193771 2.94573193719288e-07 NA NA NA hsa-miR-7-1-3p CCND2 -0.0894475316162393 0.37322359785108 0.278072058804804 0.00230941164668614 mirMAP -0.150925481137646 2.69591030726187e-05 NA NA NA hsa-miR-192-5p CCNE1 -0.0426540615456616 0.621616927627554 0.253536339251842 6.27968052141139e-05 miRNAWalker2_validate -0.134085089321587 0.000290914914800468 NA NA NA hsa-miR-195-5p CCNE1 -0.00728332132631593 0.92969850577609 0.253536339251842 6.27968052141139e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.187859421689644 1.50466938767298e-07 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-497-5p CCNE1 -0.000783764887886562 0.991703566618871 0.253536339251842 6.27968052141139e-05 MirTarget;miRNATAP -0.107990353660474 0.00593270549342151 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-30a-5p CCNE2 -0.062942936611238 0.513851954700252 0.318474669342545 0.000263822187487161 miRNATAP -0.404174405891182 3.32123969313609e-10 NA NA NA hsa-miR-30d-5p CCNE2 -0.0607010868720046 0.471410043548794 0.318474669342545 0.000263822187487161 miRNATAP -0.248704351406826 0.000276173784789898 25843294 MicroRNA 30d 5p inhibits tumour cell proliferation and motility by directly targeting CCNE2 in non small cell lung cancer; In addition the re-introduction of CCNE2 expression antagonised the inhibitory effects of miR-30d-5p on the capacity of NSCLC cells for proliferation and motility motility lung squamous cell cancer hsa-miR-103a-3p CDK2 -0.147443193527362 0.128400914654149 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate -0.244529342446133 9.50719675121511e-05 NA NA NA hsa-miR-124-3p CDK2 -0.506026514549291 0.0078056370358648 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate;miRTarBase -0.168041487619791 1.69965544728722e-19 NA NA NA hsa-miR-490-3p CDK2 -0.834268051506884 5.26203010743215e-06 0.813366814153226 5.60398748524507e-23 miRanda -0.197884061603562 5.32644876787653e-25 NA NA NA hsa-miR-495-3p CDK2 -0.421982588962504 0.00504424038526901 0.813366814153226 5.60398748524507e-23 mirMAP -0.162294675651709 1.05494965345435e-11 NA NA NA hsa-miR-7-1-3p CDK2 -0.0894475316162393 0.37322359785108 0.813366814153226 5.60398748524507e-23 mirMAP -0.215889061968793 2.42828507111814e-09 NA NA NA hsa-miR-885-5p CDK2 -0.423614695846868 1.14213289528402e-05 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate;miRTarBase -0.319170723509576 1.73805882382479e-17 NA NA NA hsa-miR-124-3p CDK4 -0.506026514549291 0.0078056370358648 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.149095153225998 7.48747415901698e-17 25348738;25731732;27323123 MiR 124 retards bladder cancer growth by directly targeting CDK4; In order to investigate the physiological role of miR-124 in bladder cancer target genes of miR-124 were predicted by the TargetScan software and cyclin-dependent kinase CDK4 which has been implicated as a regulator of cell cycle was chosen for further study; MiR-124 could significantly repress CDK4 expression by targeting its binding site in the 3'UTR of CDK4 in vitro; In both bladder cancer cell lines and tissues the expression of miR-124 was significantly down-regulated while CDK4 expression was up-regulated; And the expression of miR-124 and CDK4 showed an obvious inverse correlation in these xenograft tissues which was also observed in human bladder cancer tissue samples; Taken together our results strongly suggest that miR-124 can arrest cell cycle and restrain the growth of bladder cancer by targeting CDK4 directly;MiR 124 inhibits cell proliferation in breast cancer through downregulation of CDK4; We identified and confirmed that cyclin-dependent kinase 4 CDK4 was a direct target of miR-124; Overexpression of miR-124 suppressed CDK4 protein expression and attenuated cell viability proliferation and cell cycle progression in MCF-7 and MDA-MB-435S breast cancer cells in vitro; Overexpression of CDK4 partially rescued the inhibitory effect of miR-124 in the breast cancer cells; Our results demonstrate that miR-124 functions as a growth-suppressive miRNA and plays an important role in inhibiting tumorigenesis by targeting CDK4;miR 124 radiosensitizes human esophageal cancer cell TE 1 by targeting CDK4; Finally we identified that CDK4 is a direct target of miR-124 in TE-1 cells using target prediction algorithms and a luciferase reporter assay; Moreover western blot assay confirmed that CDK4 was downregulated during miR-124 transfection ;progression;tumorigenesis; bladder cancer;breast cancer;esophageal cancer hsa-miR-145-5p CDK4 0.108948399943015 0.310032352776693 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.175782720088333 2.61540642411898e-07 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-24-3p CDK4 0.133257261462871 0.212960921400853 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.144306679535031 6.44290009253847e-05 NA NA NA hsa-miR-34a-5p CDK4 0.143472919835524 0.211283228567057 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.118448417522733 0.000139347458480003 25789847 Real-time PCR and western blot analysis of extracted RNA and total protein revealed artemsinin and artesunate increased miR-34a expression in a dose-dependent manner correlating with down-regulation of the miR-34a target gene CDK4; Phytochemical treatments inhibited the luciferase activity of a construct containing the wild-type 3'UTR of CDK4 but not those with a mutated miR-34a binding site whereas transfection of miR-34a inhibitors ablated the phytochemical mediated down-regulation of CDK4 and induction of cell cycle arrest breast cancer hsa-miR-766-3p CDK4 -0.147747825439875 0.266658981351507 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate -0.211336637770052 5.92406894308187e-17 NA NA NA hsa-miR-101-3p CDK6 -0.132333931567244 0.241891922291381 0.514594252893836 5.86679673505214e-06 mirMAP -0.214050983778852 0.000115517087379797 NA NA NA hsa-miR-1179 CDK6 -0.117056451908751 0.310989525634765 0.514594252893836 5.86679673505214e-06 PITA;miRNATAP -0.142889521672303 0.000881663691628466 NA NA NA hsa-miR-1224-5p CDK6 -0.53941068839383 0.00201058335769201 0.514594252893836 5.86679673505214e-06 miRNATAP -0.101570856158794 0.000240685196252074 NA NA NA hsa-miR-129-5p CDK6 -0.567133990117126 0.00116152215679936 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate -0.103420012068894 0.00024955678235129 24055727 Interestingly we showed that cyclin dependent kinase 6 CDK6 a cell cycle-associated protein involved in G1-S transition was a target of miR-129 gastric cancer hsa-miR-137 CDK6 -0.924849266740909 1.546832202049e-06 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.100134233590921 5.13631857844561e-05 18577219;25342326;23178712;21051724 Transfection of microRNA-124 or microRNA-137 also induced G1 cell cycle arrest in U251 and SF6969 glioblastoma multiforme cells which was associated with decreased expression of cyclin-dependent kinase 6 and phosphorylated retinoblastoma pSer 807/811 proteins;Bioinformatics prediction and luciferase reporter assay revealed CDK6 as a target gene through which miR-137 exerted an inhibitory function;miR 137 inhibits the proliferation of lung cancer cells by targeting Cdc42 and Cdk6; Ectopic expression of miR-137 in lung cancer cells significantly downregulated Cdc42 Cdk6 and induced G1 cell cycle arrest leading to a significant decrease in cell growth in vivo and in vitro; Further both Cdc42 and Cdk6 were confirmed as targets of miR-137;The results showed that miR-137 can act as a tumor suppressor in uveal melanoma cell proliferation through downregulation of the targets MITF and CDK6 miR-137 may be epigenetically silenced during uveal melanoma tumorigenesis ;;;tumorigenesis glioblastoma;gastric cancer;lung cancer;melanoma hsa-miR-140-3p CDK6 -0.123864258867705 0.14796686619449 0.514594252893836 5.86679673505214e-06 miRNATAP -0.26390527008378 0.000421888747453862 NA NA NA hsa-miR-29a-3p CDK6 -0.224140851576083 0.0349516103550236 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.295012706425205 5.99888048504157e-07 22493297;23245396;20086245 In addition a target of miR-29a cyclin-dependent kinase 6 gene and a target of miR-142-3p TGF-β-activated kinase 1/MAP3K7 binding protein 2 gene are involved in the regulation of both monocytic and granulocytic differentiation;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL differentiation;; acute myeloid leukemia;melanoma;lymphoma hsa-miR-29b-3p CDK6 -0.178522712044497 0.0856106432165465 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.269230638368595 2.03842569918004e-07 26180082;23245396;25472644;23591808;27230400;20086245 Knockdown of NTSR1 increased the expression of miR-29b-1 and miR-129-3p which were responsible for the decreased CDK6 expression;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;Moreover miR-29b inhibited the expression of MCL1 and CDK6;Here we have identified the oncogene cyclin-dependent protein kinase 6 CDK6 as a direct target of miR-29b in lung cancer;MiR 29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6; In this study we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods; We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells; Furthermore we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues; The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL ;;;;; glioblastoma;melanoma;colorectal cancer;lung cancer;sarcoma;lymphoma hsa-miR-29c-3p CDK6 -0.372180390387259 0.00202634431973208 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.208117488470636 2.11482006457337e-06 26396669 Furthermore through qPCR and Western blot assays confirmed that overexpression of miR-29c reduced CDK6 mRNA and protein levels; miR-29c could inhibit the proliferation migration and invasion of bladder cancer cells via regulating CDK6 bladder cancer hsa-miR-30a-5p CDK6 -0.062942936611238 0.513851954700252 0.514594252893836 5.86679673505214e-06 mirMAP -0.216479485176302 0.00926959328929756 NA NA NA hsa-miR-30b-5p CDK6 -0.0858101016322106 0.263243418521854 0.514594252893836 5.86679673505214e-06 mirMAP -0.264336978353372 0.000515451713470756 NA NA NA hsa-miR-30d-3p CDK6 0.0854049347792898 0.20691254801121 0.514594252893836 5.86679673505214e-06 mirMAP -0.200436058332701 0.00560114486149501 NA NA NA hsa-miR-30d-5p CDK6 -0.0607010868720046 0.471410043548794 0.514594252893836 5.86679673505214e-06 mirMAP -0.300718358106314 0.000558788158739809 NA NA NA hsa-miR-338-3p CDK6 -0.39963704679384 0.0034410643220691 0.514594252893836 5.86679673505214e-06 mirMAP -0.123780088353193 0.00144999386822314 NA NA NA hsa-miR-362-5p CDK6 -0.0730185557229279 0.425572997292224 0.514594252893836 5.86679673505214e-06 mirMAP -0.16543927930901 0.0021247110211983 NA NA NA hsa-miR-488-5p CDK6 -0.385755602369049 7.99431673275965e-08 0.514594252893836 5.86679673505214e-06 mirMAP -0.265214262784328 0.000150929371106913 NA NA NA hsa-miR-491-3p CDK6 -0.523374665885358 1.93479565404951e-05 0.514594252893836 5.86679673505214e-06 PITA;miRNATAP -0.14151833133198 0.000308714771991301 NA NA NA hsa-miR-660-5p CDK6 -0.138989893523975 0.0945491063873448 0.514594252893836 5.86679673505214e-06 mirMAP -0.206589176560963 0.00107106139495866 NA NA NA hsa-miR-7-1-3p CDK6 -0.0894475316162393 0.37322359785108 0.514594252893836 5.86679673505214e-06 mirMAP -0.161897757484807 0.000856176984301135 NA NA NA hsa-miR-767-5p CDK6 -0.551653504513145 2.61109058244854e-05 0.514594252893836 5.86679673505214e-06 mirMAP -0.218990178100663 3.12583485290879e-09 NA NA NA hsa-miR-885-5p CDK6 -0.423614695846868 1.14213289528402e-05 0.514594252893836 5.86679673505214e-06 mirMAP -0.261342311783077 2.73713377344836e-07 NA NA NA hsa-miR-935 CDK6 -0.277848050075718 0.02166231531395 0.514594252893836 5.86679673505214e-06 mirMAP -0.11716238994515 0.00413414436299781 NA NA NA hsa-let-7c-5p CDKN1A -0.04295851886768 0.676052842748691 0.427121917792729 0.00123136007948734 MirTarget -0.323059985687549 1.83340518666355e-05 NA NA NA hsa-let-7i-5p CDKN1A 0.0952853587923244 0.192120688957249 0.427121917792729 0.00123136007948734 MirTarget -0.290880872793201 0.00310217886465504 NA NA NA hsa-miR-106b-5p CDKN1A 0.167096183547615 0.0497544396383451 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.354916178485011 1.93698403552949e-06 NA NA NA hsa-miR-125a-5p CDKN1A -0.0833349424517387 0.325440200253607 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase -0.417635698065873 2.90152784450923e-07 NA NA NA hsa-miR-129-2-3p CDKN1A -0.6175132197013 0.000561573620904867 0.427121917792729 0.00123136007948734 mirMAP -0.132493112138164 2.17805696270136e-05 NA NA NA hsa-miR-17-5p CDKN1A 0.0975751369167082 0.20621110214601 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.455125254344935 4.24790487714932e-07 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-455-3p CDKN1A 0.512701816402104 0.000459755983280155 0.427121917792729 0.00123136007948734 MirTarget -0.108213069650159 0.00527030056074479 NA NA NA hsa-miR-491-5p CDKN1A -0.749881113074983 8.22859263179194e-09 0.427121917792729 0.00123136007948734 miRanda -0.187701364863274 8.68850198681925e-06 NA NA NA hsa-miR-505-5p CDKN1A 0.0973008785018434 0.183209581426791 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;MirTarget -0.381395926281218 9.16666243828523e-07 NA NA NA hsa-miR-708-5p CDKN1A -0.00936519718301376 0.919857671524527 0.427121917792729 0.00123136007948734 MirTarget -0.340713510326019 2.02954343368862e-07 NA NA NA hsa-miR-93-5p CDKN1A 0.139766237971122 0.101057320076629 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.407487489770916 6.20600753969256e-05 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-98-5p CDKN1A -0.0806971683871618 0.251777941211683 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;MirTarget -0.306627890186986 0.000734149322136236 NA NA NA hsa-miR-148a-3p CDKN1B 0.306821592493439 0.0392356958885949 -0.0903104874713394 0.0907488975491606 miRNATAP -0.104087004262967 7.76609987083251e-22 NA NA NA hsa-miR-221-3p CDKN1B -0.0109507285071775 0.936440693394519 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.110743557621931 6.97784252871238e-23 19953484;17721077;23637992;23939688;19126397;20146005;23967190;17569667;22992757;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;progression;;;staging;tumorigenesis;;;;poor survival glioblastoma;glioblastoma;breast cancer;breast cancer;melanoma;liver cancer;pancreatic cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-222-3p CDKN1B 0.107117065575197 0.423818119725697 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.105894357261447 1.7642291217018e-20 19953484;26912358;24895988;24137356;17569667;27282281;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Besides microvesicle marker characterization we evidenced that miR-222 exosomal expression mostly reflected its abundance in the cells of origin correctly paralleled by repression of its target genes such as p27Kip1 and induction of the PI3K/AKT pathway thus confirming its functional implication in cancer;MiR-222 plays an important role in the tumorigenesis of CC possibly by specifically down-regulating p27Kip1 and PTEN;miR 222 is upregulated in epithelial ovarian cancer and promotes cell proliferation by downregulating P27kip1; miR-222 upregulation induced an enhancement of ovarian cancer cell proliferation potential possibly by downregulating its target P27Kip1; A bioinformatic analysis showed that the 3'-UTR of the P27Kip1 mRNA contained a highly-conserved putative miR-222 binding site; Luciferase reporter assays demonstrated that P27Kip1 was a direct target of miR-222; Consistently there was an inverse correlation between the P27Kip1 and miR-222 expression levels in the ovarian cancer cell lines and tissues;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;miR 222 confers the resistance of breast cancer cells to Adriamycin through suppression of p27kip1 expression; Immunofluorescence showed that miR-222 altered the subcellular location of p27kip1 in nucleus; The results showed that downregulation of miR-222 in MCF-7/Adr increased sensitivity to Adr and Adr-induced apoptosis and arrested the cells in G1 phase accompanied by more expressions of p27kip1 especially in nucleus; Taken together the results found that miR-222 induced Adr-resistance at least in part via suppressing p27kip1 expression and altering its subcellular localization and miR-222 inhibitors could reverse Adr-resistance of breast cancer cells;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;tumorigenesis;;;drug resistance;poor survival glioblastoma;melanoma;cervical and endocervical cancer;ovarian cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-24-3p CDKN1B 0.133257261462871 0.212960921400853 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate;miRNATAP -0.113888072566706 2.15931284116347e-12 26847530;26044523 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated;With the bioinformatic method we further identified that p27Kip1 is a direct target of miR-24-3p and its protein level was negatively regulated by miR-24-3p ; prostate cancer;breast cancer hsa-miR-30a-3p CDKN1B 0.0655410309550977 0.509541256241954 -0.0903104874713394 0.0907488975491606 MirTarget -0.135932822746313 3.76783512403037e-11 NA NA NA hsa-miR-30e-3p CDKN1B 0.00173769765874887 0.984965501547409 -0.0903104874713394 0.0907488975491606 MirTarget -0.126819201706031 5.97208515819098e-08 NA NA NA hsa-miR-455-5p CDKN1B 0.332224239762 0.0106491358056238 -0.0903104874713394 0.0907488975491606 miRanda;miRNATAP -0.103724305413802 2.1331574276816e-18 NA NA NA hsa-miR-98-5p CDKN1B -0.0806971683871618 0.251777941211683 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate -0.161056976459826 1.93301457144278e-10 NA NA NA hsa-miR-199b-5p CHRM1 0.436298593972862 0.00200225454383871 -0.879687677188821 4.42563456948618e-06 miRanda -0.160231909874257 0.00723970112681652 NA NA NA hsa-miR-27a-3p CHRM1 0.303760032895792 0.00457375391189806 -0.879687677188821 4.42563456948618e-06 miRNATAP -0.734360860258017 6.9051136425485e-19 NA NA NA hsa-miR-30c-2-3p CHRM1 -0.18630560418806 0.0313113706136533 -0.879687677188821 4.42563456948618e-06 mirMAP -0.467525092422019 1.07440706962604e-05 NA NA NA hsa-miR-339-5p CHRM1 0.273253751377152 0.0185300170790638 -0.879687677188821 4.42563456948618e-06 miRanda -0.349682371872257 1.40595797631218e-06 NA NA NA hsa-miR-542-3p CHRM2 0.0835995677900829 0.403838744494071 -0.461445123465205 0.0114599246970221 miRanda -0.284443048105413 0.00129942438504849 NA NA NA hsa-miR-15a-5p CHUK 0.142658985373611 0.0536219185009004 -0.0805827732736795 0.0444542176915521 miRNAWalker2_validate;miRTarBase;MirTarget -0.106856768478998 8.98534595935875e-07 NA NA NA hsa-miR-15b-5p CHUK 0.592863156692837 1.41971517354055e-10 -0.0805827732736795 0.0444542176915521 MirTarget -0.133202016968096 1.16700126521113e-19 NA NA NA hsa-miR-124-3p COL1A1 -0.506026514549291 0.0078056370358648 1.23998298011727 1.14065510873293e-09 miRNAWalker2_validate;mirMAP -0.274473693173072 5.35141345558867e-09 NA NA NA hsa-miR-129-2-3p COL1A1 -0.6175132197013 0.000561573620904867 1.23998298011727 1.14065510873293e-09 mirMAP -0.346072907997388 2.12119777773723e-12 NA NA NA hsa-miR-129-5p COL1A1 -0.567133990117126 0.00116152215679936 1.23998298011727 1.14065510873293e-09 miRanda -0.377496478237911 2.64782833300606e-13 NA NA NA hsa-miR-133a-3p COL1A1 -0.335058908302964 0.0180402783121034 1.23998298011727 1.14065510873293e-09 miRNAWalker2_validate -0.164355503397801 0.00949277474051173 NA NA NA hsa-miR-218-5p COL1A1 -0.534287404275829 0.00308322441944796 1.23998298011727 1.14065510873293e-09 MirTarget;miRNATAP -0.196467261308336 6.9700759686819e-05 NA NA NA hsa-miR-29a-3p COL1A1 -0.224140851576083 0.0349516103550236 1.23998298011727 1.14065510873293e-09 miRNATAP -0.371642854777004 0.000801619915147498 NA NA NA hsa-miR-29c-3p COL1A1 -0.372180390387259 0.00202634431973208 1.23998298011727 1.14065510873293e-09 miRNAWalker2_validate;miRTarBase;miRNATAP -0.326657793697716 6.84727320501142e-05 NA NA NA hsa-miR-330-3p COL1A1 -0.427388106938151 0.00300489284414266 1.23998298011727 1.14065510873293e-09 mirMAP -0.294327210693087 1.96552274542298e-06 NA NA NA hsa-miR-338-3p COL1A1 -0.39963704679384 0.0034410643220691 1.23998298011727 1.14065510873293e-09 miRanda;miRNATAP -0.450502875303921 2.83790953897927e-10 NA NA NA hsa-miR-577 COL1A1 -0.382068371859275 0.0145513192729119 1.23998298011727 1.14065510873293e-09 MirTarget;PITA;miRNATAP -0.241453460391243 3.09144442047382e-05 NA NA NA hsa-miR-935 COL1A1 -0.277848050075718 0.02166231531395 1.23998298011727 1.14065510873293e-09 PITA -0.394772293015598 1.67232518586797e-07 NA NA NA hsa-miR-129-5p COL1A2 -0.567133990117126 0.00116152215679936 0.945719713706391 9.33034616922201e-13 miRanda -0.180359652485836 8.27568981417803e-08 NA NA NA hsa-miR-29c-3p COL1A2 -0.372180390387259 0.00202634431973208 0.945719713706391 9.33034616922201e-13 miRNAWalker2_validate;miRTarBase;miRNATAP -0.158253676789885 0.00283427452288408 NA NA NA hsa-miR-330-5p COL1A2 -0.262932095995605 0.0465226565966658 0.945719713706391 9.33034616922201e-13 miRanda -0.125110383739967 0.00451257429123947 NA NA NA hsa-miR-490-3p COL1A2 -0.834268051506884 5.26203010743215e-06 0.945719713706391 9.33034616922201e-13 miRanda -0.195400455357079 5.48117127882046e-10 NA NA NA hsa-miR-767-5p COL1A2 -0.551653504513145 2.61109058244854e-05 0.945719713706391 9.33034616922201e-13 PITA;miRNATAP -0.235415237340486 1.22979399258405e-07 NA NA NA hsa-miR-152-3p COL2A1 -0.170744204297152 0.118543672664884 0.746239231739599 0.000374266306664883 MirTarget -0.256760772751945 0.00426035061423565 NA NA NA hsa-miR-29a-3p COL2A1 -0.224140851576083 0.0349516103550236 0.746239231739599 0.000374266306664883 MirTarget;miRNATAP -0.319762642928728 0.00484706232535909 NA NA NA hsa-miR-29b-3p COL2A1 -0.178522712044497 0.0856106432165465 0.746239231739599 0.000374266306664883 MirTarget;miRNATAP -0.319053515243603 0.00134322220471127 NA NA NA hsa-miR-124-3p COL4A1 -0.506026514549291 0.0078056370358648 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate;miRNATAP -0.220143731041708 7.51852180978931e-10 NA NA NA hsa-miR-129-5p COL4A1 -0.567133990117126 0.00116152215679936 1.37431841954319 3.47482076750947e-19 miRanda -0.32153127115203 1.89727248428967e-16 NA NA NA hsa-miR-137 COL4A1 -0.924849266740909 1.546832202049e-06 1.37431841954319 3.47482076750947e-19 miRanda -0.320596630925449 3.40808309589293e-21 NA NA NA hsa-miR-218-5p COL4A1 -0.534287404275829 0.00308322441944796 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate -0.205445314697783 3.87694837471037e-08 NA NA NA hsa-miR-29a-3p COL4A1 -0.224140851576083 0.0349516103550236 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate;miRTarBase;miRNATAP -0.380157767698226 6.16882636437929e-06 NA NA NA hsa-miR-29b-3p COL4A1 -0.178522712044497 0.0856106432165465 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate;miRTarBase;miRNATAP -0.351360819517442 1.87672188936429e-06 NA NA NA hsa-miR-29c-3p COL4A1 -0.372180390387259 0.00202634431973208 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate;miRTarBase;miRNATAP -0.338979855521423 4.78557133695897e-08 NA NA NA hsa-miR-3065-5p COL4A1 -0.262608140481507 0.0414570974148034 1.37431841954319 3.47482076750947e-19 miRNATAP -0.165314340392216 0.00201928760917277 NA NA NA hsa-miR-326 COL4A1 -0.631050656251074 8.83643450601471e-05 1.37431841954319 3.47482076750947e-19 miRanda -0.248166369489074 2.82762989851826e-09 NA NA NA hsa-miR-330-5p COL4A1 -0.262932095995605 0.0465226565966658 1.37431841954319 3.47482076750947e-19 miRanda -0.169099479618787 0.00115978611437664 NA NA NA hsa-miR-346 COL4A1 -0.577786425963268 2.10883270131593e-09 1.37431841954319 3.47482076750947e-19 miRanda -0.649569888242356 1.31729453599891e-21 NA NA NA hsa-miR-490-3p COL4A1 -0.834268051506884 5.26203010743215e-06 1.37431841954319 3.47482076750947e-19 miRanda;miRNATAP -0.318273917640076 3.52952597375553e-18 NA NA NA hsa-miR-543 COL4A1 -0.347737549779625 0.0157394585079683 1.37431841954319 3.47482076750947e-19 miRanda -0.154824393055625 0.00148934658101073 NA NA NA hsa-miR-628-5p COL4A1 -0.0469793532265896 0.657420003383685 1.37431841954319 3.47482076750947e-19 PITA;miRNATAP -0.199016143250972 0.00269857696715821 NA NA NA hsa-miR-767-5p COL4A1 -0.551653504513145 2.61109058244854e-05 1.37431841954319 3.47482076750947e-19 PITA;miRNATAP -0.265584551412774 4.71075133790993e-07 NA NA NA hsa-miR-769-3p COL4A1 -0.287263649023213 0.018145224330845 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate -0.208662411186863 0.000214614363506648 NA NA NA hsa-miR-99a-5p COL4A1 -0.120081919053582 0.258277048014275 1.37431841954319 3.47482076750947e-19 miRNAWalker2_validate -0.289991147108519 0.000384081578368666 NA NA NA hsa-miR-29a-3p COL4A2 -0.224140851576083 0.0349516103550236 1.20726274584922 7.43650937307398e-19 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.281339814035671 0.000148706268297105 NA NA NA hsa-miR-29b-3p COL4A2 -0.178522712044497 0.0856106432165465 1.20726274584922 7.43650937307398e-19 miRTarBase;MirTarget;miRNATAP -0.242498314795871 0.000195424997065481 NA NA NA hsa-miR-29c-3p COL4A2 -0.372180390387259 0.00202634431973208 1.20726274584922 7.43650937307398e-19 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.254241114757276 3.52611448605167e-06 NA NA NA hsa-miR-9-5p COL4A2 -0.125377971246422 0.269896477802284 1.20726274584922 7.43650937307398e-19 MirTarget;miRNATAP -1.11063677871337 6.21799721119648e-18 NA NA NA hsa-miR-146b-5p COL4A3 0.441078059667297 0.000237619929889385 0.0260305519356852 0.857200565210629 miRanda -0.14710066647668 0.00777736647776732 NA NA NA hsa-miR-17-5p COL4A3 0.0975751369167082 0.20621110214601 0.0260305519356852 0.857200565210629 MirTarget;TargetScan;miRNATAP -0.368867184077733 0.000286407557576239 NA NA NA hsa-miR-186-5p COL4A3 0.0606381894130505 0.514217717592464 0.0260305519356852 0.857200565210629 mirMAP -0.328389911961825 1.12590155177427e-05 NA NA NA hsa-miR-200b-3p COL4A3 0.245768526504511 0.0580744465744737 0.0260305519356852 0.857200565210629 TargetScan -0.154160097031811 0.00193388174720898 NA NA NA hsa-miR-3065-3p COL4A3 -0.261348288537884 0.0343753235107924 0.0260305519356852 0.857200565210629 miRNATAP -0.181657148314027 0.000699497219471473 NA NA NA hsa-miR-374a-5p COL4A3 0.0121037060909277 0.865417963345881 0.0260305519356852 0.857200565210629 MirTarget -0.256864610233271 0.00734464238183579 NA NA NA hsa-miR-542-3p COL4A3 0.0835995677900829 0.403838744494071 0.0260305519356852 0.857200565210629 miRanda -0.21542076412173 0.00210449491763754 NA NA NA hsa-miR-93-3p COL4A3 0.0778767956494004 0.272224943247293 0.0260305519356852 0.857200565210629 miRNATAP -0.315445692202164 0.000729556483523575 NA NA NA hsa-miR-93-5p COL4A3 0.139766237971122 0.101057320076629 0.0260305519356852 0.857200565210629 MirTarget;miRNATAP -0.33716642525711 0.00328877161912781 NA NA NA hsa-let-7a-3p COL4A4 0.0530563276543763 0.370749834463566 -0.00930206723821536 0.937042673353182 mirMAP -0.296376036114332 0.00225555992655532 NA NA NA hsa-let-7f-1-3p COL4A4 0.110713086694053 0.21484524300518 -0.00930206723821536 0.937042673353182 mirMAP -0.166916264427455 0.00366859781708009 NA NA NA hsa-miR-107 COL4A4 -0.121070851008387 0.198381281894242 -0.00930206723821536 0.937042673353182 miRanda -0.190193358082937 0.000992504493469171 NA NA NA hsa-miR-125a-3p COL4A4 0.183834985432376 0.106230647107917 -0.00930206723821536 0.937042673353182 miRanda -0.216071969601526 1.86746721138786e-06 NA NA NA hsa-miR-186-5p COL4A4 0.0606381894130505 0.514217717592464 -0.00930206723821536 0.937042673353182 mirMAP -0.420739578977158 1.90705646445698e-12 NA NA NA hsa-miR-23a-3p COL4A4 0.334693010651106 0.00144448147063433 -0.00930206723821536 0.937042673353182 miRNATAP -0.242331645803799 1.18857713076587e-05 NA NA NA hsa-miR-23b-3p COL4A4 0.0476610245860094 0.630277683372344 -0.00930206723821536 0.937042673353182 miRNATAP -0.335198005622759 5.25117418444695e-09 NA NA NA hsa-miR-26b-5p COL4A4 -0.0984536538762768 0.231803769574623 -0.00930206723821536 0.937042673353182 miRNAWalker2_validate -0.308920484449589 3.30310183992152e-05 NA NA NA hsa-miR-299-5p COL4A4 -0.0814737109317336 0.57620244226785 -0.00930206723821536 0.937042673353182 miRNATAP -0.115553389485561 0.001173459410716 NA NA NA hsa-miR-29b-3p COL4A4 -0.178522712044497 0.0856106432165465 -0.00930206723821536 0.937042673353182 MirTarget;miRNATAP -0.227807945311952 3.618034191508e-05 NA NA NA hsa-miR-301a-3p COL4A4 0.00956875714542704 0.916380325220259 -0.00930206723821536 0.937042673353182 mirMAP -0.342223107142664 4.18556853048064e-09 NA NA NA hsa-miR-30d-3p COL4A4 0.0854049347792898 0.20691254801121 -0.00930206723821536 0.937042673353182 MirTarget -0.257822900237862 0.000752504730403653 NA NA NA hsa-miR-30e-3p COL4A4 0.00173769765874887 0.984965501547409 -0.00930206723821536 0.937042673353182 MirTarget -0.292534413142119 0.000117734037514228 NA NA NA hsa-miR-361-5p COL4A4 0.0107519600643302 0.882230614095565 -0.00930206723821536 0.937042673353182 MirTarget;miRanda -0.459857360180392 1.30540575759013e-07 NA NA NA hsa-miR-376a-5p COL4A4 -0.0877544252790136 0.551096721204598 -0.00930206723821536 0.937042673353182 mirMAP -0.180923578621361 2.86440480967046e-07 NA NA NA hsa-miR-454-3p COL4A4 0.177318549827649 0.0120865170334418 -0.00930206723821536 0.937042673353182 mirMAP -0.208253484907011 0.00608848222685136 NA NA NA hsa-miR-488-3p COL4A4 -0.257955761012568 0.00280795162806256 -0.00930206723821536 0.937042673353182 MirTarget -0.158676901358968 0.00908665620985713 NA NA NA hsa-miR-491-3p COL4A4 -0.523374665885358 1.93479565404951e-05 -0.00930206723821536 0.937042673353182 PITA;miRNATAP -0.15415904867618 0.00020618512171712 NA NA NA hsa-miR-501-5p COL4A4 0.0878951859817452 0.309663377222443 -0.00930206723821536 0.937042673353182 mirMAP -0.194229307460825 0.00111477971422699 NA NA NA hsa-miR-576-5p COL4A4 0.106482276040047 0.194379152564166 -0.00930206723821536 0.937042673353182 PITA -0.389834073675672 4.60970326200937e-10 NA NA NA hsa-miR-589-3p COL4A4 0.00739371736361305 0.927768051649329 -0.00930206723821536 0.937042673353182 mirMAP -0.241359280960214 0.000142172014821868 NA NA NA hsa-miR-590-3p COL4A4 0.312951515438396 0.000480455423843996 -0.00930206723821536 0.937042673353182 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.24593208705996 1.56802030646629e-05 NA NA NA hsa-miR-767-5p COL4A5 -0.551653504513145 2.61109058244854e-05 -0.339466575628795 0.00289469837336238 PITA;miRNATAP -0.172221497912524 7.4576511227442e-06 NA NA NA hsa-miR-346 COL4A6 -0.577786425963268 2.10883270131593e-09 -0.110260766816312 0.516071453837067 miRanda -0.203457254074775 0.00785159362231278 NA NA NA hsa-miR-490-3p COL4A6 -0.834268051506884 5.26203010743215e-06 -0.110260766816312 0.516071453837067 miRanda -0.121177662361868 0.00301903189420177 NA NA NA hsa-miR-582-3p COL4A6 -0.11807589183011 0.304077332003677 -0.110260766816312 0.516071453837067 PITA -0.352518017949233 8.15250327009382e-08 NA NA NA hsa-miR-96-5p COL4A6 -0.217255611810229 0.209932800103896 -0.110260766816312 0.516071453837067 TargetScan -0.147466636330223 0.000639000363111825 NA NA NA hsa-miR-1301-3p COL6A1 -0.105695714210758 0.152249184194132 0.377858039464401 1.79900459769883e-06 miRNAWalker2_validate -0.113400971703022 0.00911520939544114 NA NA NA hsa-miR-124-3p COL6A2 -0.506026514549291 0.0078056370358648 1.02041936795331 5.13715520230763e-14 miRNAWalker2_validate -0.10514655378741 0.000945145918679589 NA NA NA hsa-miR-129-2-3p COL6A2 -0.6175132197013 0.000561573620904867 1.02041936795331 5.13715520230763e-14 mirMAP -0.142848376691268 1.92595482485689e-05 NA NA NA hsa-miR-184 COL6A2 -0.842534984406592 6.08844932016991e-08 1.02041936795331 5.13715520230763e-14 miRanda -0.139619509943841 0.000279767195163777 NA NA NA hsa-miR-767-5p COL6A3 -0.551653504513145 2.61109058244854e-05 0.780087787207954 4.19040195033263e-06 MirTarget;PITA;miRNATAP -0.184680870356605 0.00139658137829875 NA NA NA hsa-miR-3065-5p COL9A1 -0.262608140481507 0.0414570974148034 0.283407980094404 0.172011189594706 mirMAP -0.52529072468886 3.44037160478168e-14 NA NA NA hsa-miR-103a-3p CREB1 -0.147443193527362 0.128400914654149 0.0701410893844638 0.0991640818647242 miRNAWalker2_validate -0.154152780145284 3.35772795818526e-13 NA NA NA hsa-miR-30a-5p CREB1 -0.062942936611238 0.513851954700252 0.0701410893844638 0.0991640818647242 MirTarget;mirMAP -0.147202058153182 3.48507910254539e-12 NA NA NA hsa-miR-30b-5p CREB1 -0.0858101016322106 0.263243418521854 0.0701410893844638 0.0991640818647242 MirTarget;mirMAP -0.111009767895454 1.37752691972062e-08 NA NA NA hsa-miR-362-5p CREB1 -0.0730185557229279 0.425572997292224 0.0701410893844638 0.0991640818647242 TargetScan -0.103713358471472 3.04885842803763e-14 NA NA NA hsa-miR-184 CREB3L1 -0.842534984406592 6.08844932016991e-08 0.406905294312771 0.0356205091796311 MirTarget;miRanda;miRNATAP -0.307415500021226 9.83595723451265e-09 NA NA NA hsa-miR-124-5p CREB3L2 -0.675122821846076 2.29104160508523e-06 0.204901565293207 0.000534813133884361 mirMAP -0.140158223481273 2.90763913999304e-20 NA NA NA hsa-miR-128-3p CREB3L2 -0.781932174412672 7.1812756321342e-10 0.204901565293207 0.000534813133884361 MirTarget -0.1467252499069 8.73135412690502e-15 NA NA NA hsa-miR-346 CREB3L2 -0.577786425963268 2.10883270131593e-09 0.204901565293207 0.000534813133884361 MirTarget;miRanda -0.121323914020028 1.46877030219528e-07 NA NA NA hsa-miR-7-1-3p CREB3L2 -0.0894475316162393 0.37322359785108 0.204901565293207 0.000534813133884361 mirMAP -0.101856931282842 6.61755688777635e-06 NA NA NA hsa-miR-9-3p CREB3L2 -0.184945845984695 0.0290051908009009 0.204901565293207 0.000534813133884361 mirMAP -0.232808125758444 8.09911062569429e-11 NA NA NA hsa-miR-9-5p CREB3L2 -0.125377971246422 0.269896477802284 0.204901565293207 0.000534813133884361 miRNATAP -0.484240689259221 2.35353609321494e-24 NA NA NA hsa-miR-105-5p CREB5 -0.567459149851677 8.20858944634795e-06 0.428178216398607 4.27870132500159e-07 miRNATAP -0.131257641307965 3.05266077583895e-06 NA NA NA hsa-miR-362-5p CREB5 -0.0730185557229279 0.425572997292224 0.428178216398607 4.27870132500159e-07 PITA -0.145684580424283 0.000209894892213653 NA NA NA hsa-miR-331-3p CRTC2 0.0126984726684189 0.905175053956974 0.042808223377814 0.342206062361917 miRNATAP -0.133434608700706 7.07002207137209e-25 NA NA NA hsa-miR-361-3p CRTC2 -0.0798653643614555 0.271064714084553 0.042808223377814 0.342206062361917 miRNATAP -0.128209155948189 8.88683405728484e-09 NA NA NA hsa-miR-106a-5p CSF1 0.110074064773897 0.13045815990621 -0.122287343211195 0.20837887149901 miRNATAP -0.240789052612401 2.79463057370932e-05 NA NA NA hsa-miR-126-5p CSF1 0.177332403948664 0.0711675116243123 -0.122287343211195 0.20837887149901 MirTarget;miRNATAP -0.243683757786645 5.28079809914705e-09 NA NA NA hsa-miR-128-3p CSF1 -0.781932174412672 7.1812756321342e-10 -0.122287343211195 0.20837887149901 MirTarget -0.133621533589285 7.63301513989826e-05 22909061 By mutations in putative miRNA targets in CSF-1 mRNA 3'UTR we identified a common target for both miR-128 and miR-152; We have also found that both miR-128 and miR-152 down-regulate CSF-1 mRNA and protein expression in ovarian cancer cells leading to decreased cell motility and adhesion in vitro two major aspects of the metastatic potential of cancer cells; Our results provide the evidence for a mechanism by which miR-128 and miR-152 down-regulate CSF-1 an important regulator of ovarian cancer motility ovarian cancer hsa-miR-129-2-3p CSF1 -0.6175132197013 0.000561573620904867 -0.122287343211195 0.20837887149901 mirMAP -0.111728168442036 3.15538342899908e-07 NA NA NA hsa-miR-130b-3p CSF1 0.503911467685709 9.46590588950823e-08 -0.122287343211195 0.20837887149901 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.14220934501961 0.000833508067375941 22005523 CSF-1 regulated by miR-130b was detected using Dual Luciferase Reporter system; CSF-1 expression was negatively associated with miR-130b level in ovarian tissues and cell lines; Luciferase assay validated CSF-1 is a direct target of miR-130b; Knock-down of CSF-1 sensitized ovarian cancer cells to anticancer drugs and could partially attenuate the resistance inducing effect of miR-130b inhibitors; Downregulation of miR-130b promotes the development of multidrug resistant ovarian cancer partially by targeting the 3'-UTR of CSF-1 and the silencing of miR-130b may be mediated by DNA methylation drug resistance ovarian cancer hsa-miR-143-5p CSF1 0.460068066898162 0.000603073392139956 -0.122287343211195 0.20837887149901 miRNATAP -0.115170590463889 7.88392671050427e-05 NA NA NA hsa-miR-149-5p CSF1 -0.0535821592029952 0.657713892012548 -0.122287343211195 0.20837887149901 MirTarget -0.10633586741869 0.00147513224183315 NA NA NA hsa-miR-15a-5p CSF1 0.142658985373611 0.0536219185009004 -0.122287343211195 0.20837887149901 miRNATAP -0.176070958656433 0.00667785808743751 NA NA NA hsa-miR-15b-5p CSF1 0.592863156692837 1.41971517354055e-10 -0.122287343211195 0.20837887149901 miRNATAP -0.131247161097271 0.00354209989429657 NA NA NA hsa-miR-16-5p CSF1 0.246024668671282 0.00346042804503716 -0.122287343211195 0.20837887149901 miRNATAP -0.240169370059159 1.44063203773933e-05 NA NA NA hsa-miR-20a-5p CSF1 -0.0115865752288853 0.887069448776018 -0.122287343211195 0.20837887149901 miRNATAP -0.179677651953267 0.00209303802178455 NA NA NA hsa-miR-20b-5p CSF1 0.144541062989476 0.0992042333556543 -0.122287343211195 0.20837887149901 miRNATAP -0.134233413074022 0.0059003006187718 NA NA NA hsa-miR-335-5p CSF1 0.0938511632756978 0.336889056387694 -0.122287343211195 0.20837887149901 miRNAWalker2_validate -0.132628847486485 0.00133179436677681 NA NA NA hsa-miR-542-3p CSF3 0.0835995677900829 0.403838744494071 -0.143662984860303 0.461317655012971 miRanda -0.302218089112359 0.00130823318349244 NA NA NA hsa-miR-138-5p CSF3R -0.616087649920851 1.35200027885729e-05 0.172703978715942 0.111444824636693 MirTarget -0.139153549859984 2.30931003852574e-05 NA NA NA hsa-miR-199a-3p DDIT4 0.41226331871076 0.000497136154474528 -0.340397632296293 0.000585933470156393 MirTarget;PITA;miRNATAP -0.111921365809488 0.000693312425856822 NA NA NA hsa-miR-199b-3p DDIT4 0.411848361704393 0.000517815011892379 -0.340397632296293 0.000585933470156393 MirTarget;PITA;miRNATAP -0.11139228035532 0.000715052576128095 NA NA NA hsa-miR-200b-3p DDIT4 0.245768526504511 0.0580744465744737 -0.340397632296293 0.000585933470156393 TargetScan -0.153499343055954 3.41119820060798e-07 NA NA NA hsa-miR-22-3p DDIT4 0.0277287593466351 0.809325106832648 -0.340397632296293 0.000585933470156393 MirTarget -0.171981785044227 0.000216459041504238 NA NA NA hsa-miR-221-3p DDIT4 -0.0109507285071775 0.936440693394519 -0.340397632296293 0.000585933470156393 miRNAWalker2_validate;miRTarBase -0.145187258070452 2.6360324492418e-07 NA NA NA hsa-miR-30e-5p DDIT4 0.0764521828640934 0.41904991629622 -0.340397632296293 0.000585933470156393 MirTarget -0.272101656619712 1.98107108612766e-06 NA NA NA hsa-miR-32-5p DDIT4 0.130313603388223 0.177843055266148 -0.340397632296293 0.000585933470156393 miRNATAP -0.10594411203117 0.00931945355896724 NA NA NA hsa-miR-30c-2-3p EFNA1 -0.18630560418806 0.0313113706136533 0.337108113379891 3.81958280466138e-06 MirTarget -0.126840381701099 0.00085468789803609 NA NA NA hsa-miR-9-5p EFNA1 -0.125377971246422 0.269896477802284 0.337108113379891 3.81958280466138e-06 miRNAWalker2_validate;miRNATAP -0.223689482833842 0.00073194365247171 NA NA NA hsa-miR-26b-5p EFNA2 -0.0984536538762768 0.231803769574623 0.134152515817659 0.264314464365408 miRNAWalker2_validate -0.396298086664645 1.86292354310792e-07 NA NA NA hsa-miR-342-5p EFNA2 0.0383784541944436 0.544128449296127 0.134152515817659 0.264314464365408 MirTarget -0.316596928413528 0.000205275737143976 NA NA NA hsa-miR-361-3p EFNA2 -0.0798653643614555 0.271064714084553 0.134152515817659 0.264314464365408 mirMAP -0.796067610747988 2.08219968447408e-23 NA NA NA hsa-miR-625-5p EFNA2 -0.0264137834407145 0.802098211251158 0.134152515817659 0.264314464365408 mirMAP -0.245664901798787 8.36860083834835e-07 NA NA NA hsa-miR-27a-3p EFNA3 0.303760032895792 0.00457375391189806 -0.326007302809337 0.000202745054179435 miRNATAP -0.163937979445285 1.02039144734868e-05 NA NA NA hsa-miR-30a-5p EFNA3 -0.062942936611238 0.513851954700252 -0.326007302809337 0.000202745054179435 MirTarget;mirMAP;miRNATAP -0.244879166747197 0.000108762712167237 NA NA NA hsa-miR-34c-5p EFNA3 0.181931113688093 0.257266965617363 -0.326007302809337 0.000202745054179435 miRanda -0.14878785349847 3.25939246145383e-11 NA NA NA hsa-let-7a-3p EFNA5 0.0530563276543763 0.370749834463566 -0.482975700052229 0.00104762446218271 miRNATAP -0.354026410437298 0.00394039235299539 NA NA NA hsa-miR-19a-3p EFNA5 0.0466707843731911 0.576818938277755 -0.482975700052229 0.00104762446218271 miRNATAP -0.247080133425123 0.00240023233655997 NA NA NA hsa-miR-19b-3p EFNA5 -0.0138427450847924 0.856506310673067 -0.482975700052229 0.00104762446218271 miRNATAP -0.266279550517469 0.00609487986507698 NA NA NA hsa-miR-20a-3p EFNA5 0.102743778777997 0.19677865359751 -0.482975700052229 0.00104762446218271 mirMAP -0.336591037233355 4.34602591593842e-05 NA NA NA hsa-miR-21-3p EFNA5 1.3484283943822 2.0036022227401e-17 -0.482975700052229 0.00104762446218271 mirMAP -0.179309789782236 7.63673417478275e-06 NA NA NA hsa-miR-450b-5p EFNA5 0.270544879013992 0.00801537838499562 -0.482975700052229 0.00104762446218271 mirMAP -0.182944381071667 0.00463791636947344 NA NA NA hsa-miR-767-5p EFNA5 -0.551653504513145 2.61109058244854e-05 -0.482975700052229 0.00104762446218271 miRNATAP -0.133236971379462 0.00794163349757635 NA NA NA hsa-miR-105-5p EGFR -0.567459149851677 8.20858944634795e-06 0.375473556369618 0.0155485714375729 mirMAP -0.353346118507539 9.24874721523108e-12 NA NA NA hsa-miR-133a-3p EGFR -0.335058908302964 0.0180402783121034 0.375473556369618 0.0155485714375729 miRNAWalker2_validate;miRTarBase;MirTarget -0.240130883772277 1.69412746009746e-07 26138587;26076812;25903369;26134491;24816813;22407299;27465833;23786162 Growth of glioblastoma is inhibited by miR 133 mediated EGFR suppression; Bioinformatic analysis was performed showing that miR-133 may target the 3'-untranslated region 3'-UTR of the epidermal growth factor receptor EGFR that transduces cell growth signals; Further the protein translation inhibition of EGFR by miR-133 was confirmed by a dual luciferase reporter assay; Together these data suggest that reduced miR-133 levels in GBM tissues promotes cell growth and decreases cell apoptosis possibly through targeting mRNA of EGFR to suppress its translation;Moreover the 3'-untranslated region 3'UTR of Her-2 the epidermal growth factor receptor EGFR that transduces cell growth signals appeared to be targeted by miR-133;MiR-133a expression was negatively correlated to lymphatic metastasis r = -0.182 P = 0.042 tumor size r = -0.253 P = 0.04 clinical TNM stages r = -0.154 P = 0.087 and EGFR protein expression r = -0.612 P < 0.001;miR 133a inhibits cervical cancer growth by targeting EGFR; The epidermal growth factor receptor EGFR was confirmed to be a direct target of miR-133a in cervical cancer cells using luciferase assay and western blotting; Restoration of miR-133a inhibited EGFR expression and activated the AKT and ERK signaling pathways; These results showed that miR-133a suppresses cervical cancer growth in vitro and in vivo through targeting EGFR suggesting that miR-133a can be a potential target for the treatment of cervical cancer;MiR-133a can inhibit cell invasiveness and cell growth through suppressing the expressions of IGF-1R TGFBR1 and EGFR which then influences the downstream signaling in lung cancer cell lines;We also provide the first evidence that miR-133 may target EGFR;Epidermal growth factor receptor EGFR expression and Akt phosphorylation were significantly suppressed after miR-133a transfection by western blot detection; miR-133a suppressed cell proliferation by suppressing the expression of EGFR and the phosphorylation of Akt;Bioinformatics prediction showed that epidermal growth factor receptor EGFR is a potential target for miR-133a; A dual luciferase reporter gene assay showed that miR-133a bound to the 3' UTR of EGFR but not a mutated 3' UTR thereby down-regulating the protein expression level; Accordingly we found that expression of EGFR protein decreased with increased expression of miR-133a in MCF-7 and MDA-MB-231 cells; These results demonstrate that miR-133a which may act as a tumor suppressor in breast cancer regulates the cell cycle and proliferation in tumorigenesis by targeting EGFR through the downstream signal molecule Akt ;;staging;metastasis;tumor size;;;;;tumorigenesis glioblastoma;gastric cancer;lung squamous cell cancer;cervical and endocervical cancer;lung squamous cell cancer;prostate cancer;breast cancer;breast cancer hsa-miR-134-5p EGFR 0.0228681180463912 0.875465441075562 0.375473556369618 0.0155485714375729 MirTarget -0.131424347245258 0.0046901253546818 24440911;27241841 We show that the RTKs MET EGFR and PDGFR regulate microRNA-134 miR-134 in GBM; We find that miR-134 is downregulated in human tumors and cancer stem cells and that its expression inversely correlates with the activation of MET EGFR and PDGFR;Luciferase assays confirmed that EGFR is a direct target of miR-134; Further mechanistic investigation including RNAi and rescue experiments suggested that the down-regulation of EGFR by miR-134 partially contributes to the antiproliferative role of miR-134; Taken together our findings suggest that miR-134 inhibits non-small cell lung cancer growth by targeting the EGFR ; glioblastoma;lung squamous cell cancer hsa-miR-140-5p EGFR 0.065063507103468 0.380452509211773 0.375473556369618 0.0155485714375729 miRanda -0.331443926880394 0.000604746363683769 NA NA NA hsa-miR-141-3p EGFR 0.41120561991992 0.000393344390969055 0.375473556369618 0.0155485714375729 MirTarget -0.272211860280112 2.20526685695345e-06 26025929 Treatment with the EGFR inhibitor AG1478 or overexpression of miR141 blocked the activity of ERK downstream of EGFR and inhibited KLF8-depndent cell invasiveness proliferation and viability in cell culture and invasive growth and lung metastasis in nude mice metastasis breast cancer hsa-miR-145-5p EGFR 0.108948399943015 0.310032352776693 0.375473556369618 0.0155485714375729 miRTarBase -0.222408723457338 0.000632008424910129 21653642;21289483;19493678 EGFR signals downregulate tumor suppressors miR 143 and miR 145 in Western diet promoted murine colon cancer: role of G1 regulators; Effects of EGFR on miR-143 and miR-145 expression were assessed in murine and human colonic cells and their putative targets examined in vitro and in vivo; EGFR signals suppressed miR-143 and miR-145 in human and murine colonic cells; EGFR suppresses miR-143 and miR-145 in murine models of colon cancer;MiR 145 inhibits cell proliferation of human lung adenocarcinoma by targeting EGFR and NUDT1; We previously reported that restoration of hsa-miR-145 inhibits cancer cell growth in lung adenocarcinoma patients with epidermal growth factor receptor EGFR mutation; The mRNA expressions of EGFR and NUDT1 were significantly downregulated after miR-145 transfection in human lung adenocarcinoma cells; Our results demonstrated miR-145 in the negative regulation of EGFR and NUDT1 expressions at both mRNA and protein levels; Upregulation of miR-145 appeared to be an important gene regulation mechanism for the proliferation of lung adenocarcinoma cells and it correlated strongly with the downregulation of EGFR and NUDT1; Our findings provided new insight into the complex regulating pathway comprising of miR-145 EGFR NUDT1 and other unknown factors which function in cell proliferation but not in apoptosis;Our results also show that restoration of tumour suppressor hsa-miR-145 inhibits cancer cell growth in EGFR mutant lung adenocarcinoma ;; colon cancer;lung cancer;lung cancer hsa-miR-146b-5p EGFR 0.441078059667297 0.000237619929889385 0.375473556369618 0.0155485714375729 miRNATAP -0.182905457755479 0.00149160880673993 NA NA NA hsa-miR-148b-5p EGFR 0.127111784487801 0.188731110216917 0.375473556369618 0.0155485714375729 mirMAP -0.239934018287681 0.000528231733121031 NA NA NA hsa-miR-17-5p EGFR 0.0975751369167082 0.20621110214601 0.375473556369618 0.0155485714375729 TargetScan -0.300424779505137 0.00473688540815432 NA NA NA hsa-miR-186-5p EGFR 0.0606381894130505 0.514217717592464 0.375473556369618 0.0155485714375729 mirMAP -0.362820595408749 3.23429208060765e-06 NA NA NA hsa-miR-199a-3p EGFR 0.41226331871076 0.000497136154474528 0.375473556369618 0.0155485714375729 mirMAP -0.155171655893965 0.00605438554579962 NA NA NA hsa-miR-199b-3p EGFR 0.411848361704393 0.000517815011892379 0.375473556369618 0.0155485714375729 mirMAP -0.153587230840804 0.00647308065495246 NA NA NA hsa-miR-27b-3p EGFR -0.0599187745243146 0.58208143475992 0.375473556369618 0.0155485714375729 MirTarget;miRNATAP -0.221076471767459 0.000976089469884623 NA NA NA hsa-miR-29b-2-5p EGFR -0.26834261955727 0.00453760540520233 0.375473556369618 0.0155485714375729 mirMAP -0.358417872879751 3.99667666062669e-07 NA NA NA hsa-miR-340-5p EGFR -0.110504550202736 0.20522426193476 0.375473556369618 0.0155485714375729 mirMAP -0.346218765115495 1.66785221313902e-05 NA NA NA hsa-miR-342-3p EGFR -0.125683829770877 0.105810751640137 0.375473556369618 0.0155485714375729 miRanda -0.663917527907302 1.30767225931049e-12 NA NA NA hsa-miR-362-5p EGFR -0.0730185557229279 0.425572997292224 0.375473556369618 0.0155485714375729 mirMAP -0.425583661873956 4.49473787679401e-09 NA NA NA hsa-miR-374a-3p EGFR 0.176226170437625 0.0343958848425446 0.375473556369618 0.0155485714375729 mirMAP -0.362986532828884 0.000239618326261467 NA NA NA hsa-miR-374b-3p EGFR 0.00769406264478478 0.897944859266164 0.375473556369618 0.0155485714375729 mirMAP -0.427233704833407 0.000162731500775749 NA NA NA hsa-miR-375 EGFR -0.0268401141324031 0.858358101444697 0.375473556369618 0.0155485714375729 miRanda -0.169128003930842 0.000103586363427282 NA NA NA hsa-miR-432-5p EGFR -0.214242438141977 0.166186087759946 0.375473556369618 0.0155485714375729 mirMAP -0.131937974290007 0.0019216701815729 NA NA NA hsa-miR-455-5p EGFR 0.332224239762 0.0106491358056238 0.375473556369618 0.0155485714375729 miRanda -0.204812179021233 5.18972286460639e-05 NA NA NA hsa-miR-490-3p EGFR -0.834268051506884 5.26203010743215e-06 0.375473556369618 0.0155485714375729 miRNATAP -0.120072101708822 0.00092511979931243 NA NA NA hsa-miR-491-5p EGFR -0.749881113074983 8.22859263179194e-09 0.375473556369618 0.0155485714375729 miRanda -0.15355901980036 0.00200795950337826 25299770 This latter effect is due to direct targeting of epidermal growth factor receptor EGFR by miR-491-5p and consequent inhibition of downstream AKT and MAPK signalling pathways; Induction of apoptosis by miR-491-5p in this cell line is mimicked by a combination of EGFR inhibition together with a BH3-mimetic molecule ovarian cancer hsa-miR-501-5p EGFR 0.0878951859817452 0.309663377222443 0.375473556369618 0.0155485714375729 mirMAP -0.204458704242994 0.00778191664099004 NA NA NA hsa-miR-539-5p EGFR -0.326485413953598 0.0554453076411246 0.375473556369618 0.0155485714375729 MirTarget;mirMAP -0.164306654210725 1.88169889749939e-05 NA NA NA hsa-miR-542-3p EGFR 0.0835995677900829 0.403838744494071 0.375473556369618 0.0155485714375729 miRanda -0.200337541146819 0.00617654519167961 NA NA NA hsa-miR-577 EGFR -0.382068371859275 0.0145513192729119 0.375473556369618 0.0155485714375729 mirMAP -0.117018511597701 0.00601694200161573 NA NA NA hsa-miR-590-3p EGFR 0.312951515438396 0.000480455423843996 0.375473556369618 0.0155485714375729 mirMAP -0.214045002105238 0.00365962356824128 NA NA NA hsa-miR-654-3p EGFR -0.00355488592826836 0.97967144096285 0.375473556369618 0.0155485714375729 mirMAP -0.128189186321505 0.00719754733314809 NA NA NA hsa-miR-7-1-3p EGFR -0.0894475316162393 0.37322359785108 0.375473556369618 0.0155485714375729 mirMAP -0.276379724120531 2.83072126766495e-05 NA NA NA hsa-miR-769-5p EGFR -0.320572895263071 0.00425481410304398 0.375473556369618 0.0155485714375729 mirMAP -0.16419521509748 0.00642768950459396 NA NA NA hsa-miR-877-5p EGFR -0.0775421790642756 0.423413623033696 0.375473556369618 0.0155485714375729 mirMAP -0.212946423195282 0.00180004411848772 NA NA NA hsa-miR-103a-3p EIF4B -0.147443193527362 0.128400914654149 -0.152061905713888 0.0271875624306495 MirTarget;miRNATAP -0.283333004226312 3.03876316241244e-14 NA NA NA hsa-miR-107 EIF4B -0.121070851008387 0.198381281894242 -0.152061905713888 0.0271875624306495 MirTarget;PITA;miRanda;miRNATAP -0.129970286069417 1.54239548429985e-07 NA NA NA hsa-miR-149-5p EIF4B -0.0535821592029952 0.657713892012548 -0.152061905713888 0.0271875624306495 miRNAWalker2_validate;miRNATAP -0.165366935947129 6.22748422667344e-20 NA NA NA hsa-miR-15a-5p EIF4B 0.142658985373611 0.0536219185009004 -0.152061905713888 0.0271875624306495 miRNATAP -0.162339529170139 7.00813946895071e-06 NA NA NA hsa-miR-15b-5p EIF4B 0.592863156692837 1.41971517354055e-10 -0.152061905713888 0.0271875624306495 miRNATAP -0.169525586751993 7.37762490301615e-12 NA NA NA hsa-miR-185-5p EIF4B -0.0767432095218297 0.404113540276816 -0.152061905713888 0.0271875624306495 MirTarget -0.148325648817469 3.9428302535025e-09 NA NA NA hsa-miR-193a-3p EIF4B 0.395602305834739 0.00182611787714301 -0.152061905713888 0.0271875624306495 miRanda -0.12214409249832 2.91315524752069e-12 NA NA NA hsa-miR-193b-3p EIF4B 0.0766333116224072 0.324723824453143 -0.152061905713888 0.0271875624306495 miRNAWalker2_validate -0.156269361900307 1.53717834593784e-07 NA NA NA hsa-miR-25-3p EIF4B 0.316649850160985 0.000777982468387578 -0.152061905713888 0.0271875624306495 miRNAWalker2_validate -0.108167672109265 0.000434907765781561 NA NA NA hsa-miR-30a-3p EIF4B 0.0655410309550977 0.509541256241954 -0.152061905713888 0.0271875624306495 mirMAP -0.235668851050448 8.2988039215685e-17 NA NA NA hsa-miR-30d-3p EIF4B 0.0854049347792898 0.20691254801121 -0.152061905713888 0.0271875624306495 mirMAP -0.136809577532992 3.34445240765013e-05 NA NA NA hsa-miR-30e-3p EIF4B 0.00173769765874887 0.984965501547409 -0.152061905713888 0.0271875624306495 mirMAP -0.23335200823596 4.6813466979733e-13 NA NA NA hsa-miR-335-3p EIF4B 0.34540584335595 0.00043848660222294 -0.152061905713888 0.0271875624306495 MirTarget -0.18184925891213 1.44566813952808e-15 NA NA NA hsa-miR-339-5p EIF4B 0.273253751377152 0.0185300170790638 -0.152061905713888 0.0271875624306495 miRanda -0.154794899218979 7.83659370470759e-17 NA NA NA hsa-miR-342-3p EIF4B -0.125683829770877 0.105810751640137 -0.152061905713888 0.0271875624306495 miRanda -0.150797834917468 2.18363233869528e-06 NA NA NA hsa-miR-3682-3p EIF4B 0.113471485204649 0.266481019608971 -0.152061905713888 0.0271875624306495 MirTarget -0.146814150671814 2.6059439985854e-11 NA NA NA hsa-miR-423-3p EIF4B 0.192317829125882 0.00125622290742326 -0.152061905713888 0.0271875624306495 miRNAWalker2_validate -0.157453294180685 0.000538085259664607 NA NA NA hsa-miR-484 EIF4B -0.0190828819137732 0.802298900989652 -0.152061905713888 0.0271875624306495 miRNAWalker2_validate -0.214661788802198 2.64263949840685e-12 NA NA NA hsa-miR-532-3p EIF4B 0.150624483952741 0.0599635132177191 -0.152061905713888 0.0271875624306495 miRNATAP -0.142880972254531 5.48046209246052e-07 NA NA NA hsa-miR-590-3p EIF4B 0.312951515438396 0.000480455423843996 -0.152061905713888 0.0271875624306495 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.146889392616318 1.76276693391346e-09 NA NA NA hsa-miR-9-5p EIF4E -0.125377971246422 0.269896477802284 0.000521917441616004 0.989561920017793 MirTarget -0.206255846192919 7.77430182765833e-13 NA NA NA hsa-miR-124-3p EIF4EBP1 -0.506026514549291 0.0078056370358648 0.345125060464968 2.64227717558959e-05 miRNAWalker2_validate -0.188966455099013 9.80111350430274e-28 NA NA NA hsa-miR-125a-5p EIF4EBP1 -0.0833349424517387 0.325440200253607 0.345125060464968 2.64227717558959e-05 miRNAWalker2_validate;MirTarget;miRanda;miRNATAP -0.46148529037995 1.70110184729277e-21 26646586 Increased expression of miR-125a and miR-125b inhibited invasion and migration of SKOV3 and OVCAR-429 ovarian cancer cells and was associated with a decrease in EIF4EBP1 expression ovarian cancer hsa-miR-125b-5p EIF4EBP1 -0.0119698382910496 0.890233923197358 0.345125060464968 2.64227717558959e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.308146170722745 2.72904310773947e-07 17891175;26646586 Using a bioinformatics approach we have identified additional potential mRNA targets of one of the miRNAs miR-125b that are upregulated in prostate cancer and confirmed increased expression of one of these targets EIF4EBP1 in prostate cancer tissues;Increased expression of miR-125a and miR-125b inhibited invasion and migration of SKOV3 and OVCAR-429 ovarian cancer cells and was associated with a decrease in EIF4EBP1 expression ; prostate cancer;ovarian cancer hsa-miR-138-5p EIF4EBP1 -0.616087649920851 1.35200027885729e-05 0.345125060464968 2.64227717558959e-05 MirTarget;miRNATAP -0.193406903930313 4.6473196917631e-16 NA NA NA hsa-miR-146b-3p EIF4EBP1 0.283486177568242 0.0118918188864879 0.345125060464968 2.64227717558959e-05 miRNATAP -0.209872381456042 7.96296709524663e-12 NA NA NA hsa-miR-328-3p EIF4EBP1 -0.27021372890849 0.00243992234645524 0.345125060464968 2.64227717558959e-05 MirTarget -0.534447190350655 1.29869249787005e-40 NA NA NA hsa-miR-874-3p EIF4EBP1 -0.342846516198707 0.00227406462925841 0.345125060464968 2.64227717558959e-05 MirTarget -0.403175411742658 2.94827948965856e-44 NA NA NA hsa-miR-346 EPHA2 -0.577786425963268 2.10883270131593e-09 0.718117697865268 7.21288874557592e-12 miRanda -0.294975636384396 2.85375767140981e-10 NA NA NA hsa-miR-708-3p EPHA2 0.00458267260363954 0.962455132950708 0.718117697865268 7.21288874557592e-12 MirTarget -0.148182604049826 0.00520777648202178 NA NA NA hsa-miR-127-3p EPOR -0.164565333886312 0.2710118155902 0.0411495540224545 0.503972859095025 miRanda -0.104745571126651 6.12644572107856e-09 NA NA NA hsa-miR-126-5p F2R 0.177332403948664 0.0711675116243123 0.400354991431715 3.54417987716905e-05 mirMAP -0.124486817048792 0.00355764151759715 NA NA NA hsa-miR-129-5p F2R -0.567133990117126 0.00116152215679936 0.400354991431715 3.54417987716905e-05 MirTarget;miRanda;mirMAP -0.191278042800133 3.69845010505105e-17 NA NA NA hsa-miR-136-5p F2R -0.0792119620142504 0.581591369035763 0.400354991431715 3.54417987716905e-05 mirMAP -0.149236559361107 8.96909130770332e-08 NA NA NA hsa-miR-190a-5p F2R -0.0574993923869409 0.534047621516133 0.400354991431715 3.54417987716905e-05 MirTarget -0.208802529826502 1.26136752565094e-06 NA NA NA hsa-miR-23b-3p F2R 0.0476610245860094 0.630277683372344 0.400354991431715 3.54417987716905e-05 MirTarget -0.184311507990793 4.47367266798835e-05 NA NA NA hsa-miR-29a-5p F2R -0.0697727833812678 0.350621682973119 0.400354991431715 3.54417987716905e-05 MirTarget -0.229670391555577 2.51626597490675e-05 NA NA NA hsa-miR-342-5p F2R 0.0383784541944436 0.544128449296127 0.400354991431715 3.54417987716905e-05 MirTarget -0.291231198194674 6.55865586429551e-06 NA NA NA hsa-miR-361-5p F2R 0.0107519600643302 0.882230614095565 0.400354991431715 3.54417987716905e-05 miRanda -0.293276364207239 1.7428662697287e-05 NA NA NA hsa-miR-491-5p F2R -0.749881113074983 8.22859263179194e-09 0.400354991431715 3.54417987716905e-05 miRanda -0.266852866041892 4.57960731528225e-20 NA NA NA hsa-miR-577 F2R -0.382068371859275 0.0145513192729119 0.400354991431715 3.54417987716905e-05 MirTarget;miRNATAP -0.105310292041066 4.19189349609004e-05 NA NA NA hsa-miR-582-5p F2R -0.215764335500665 0.0760892640434509 0.400354991431715 3.54417987716905e-05 MirTarget -0.248500916960507 6.40302249956472e-15 NA NA NA hsa-miR-655-3p F2R -0.241086974063521 0.0960363954844236 0.400354991431715 3.54417987716905e-05 MirTarget -0.200748138390222 1.42272251885738e-13 NA NA NA hsa-miR-664a-3p F2R 0.0413918462467198 0.611334357897022 0.400354991431715 3.54417987716905e-05 mirMAP -0.258281000332276 2.57275049529991e-07 NA NA NA hsa-let-7d-5p FGF11 0.111345229817909 0.0872634398336815 -0.235018569265848 0.00440939153265794 mirMAP;miRNATAP -0.539705813701275 3.46462978181246e-18 NA NA NA hsa-let-7e-5p FGF11 0.0471189209570344 0.657255455444655 -0.235018569265848 0.00440939153265794 mirMAP;miRNATAP -0.272669366101863 7.34562908174005e-14 NA NA NA hsa-let-7f-5p FGF11 0.0508269185845673 0.62677515045793 -0.235018569265848 0.00440939153265794 mirMAP;miRNATAP -0.15253479478004 0.000320003174670443 NA NA NA hsa-let-7g-5p FGF11 -0.0253396334608382 0.742556489443668 -0.235018569265848 0.00440939153265794 mirMAP;miRNATAP -0.256034055595292 6.39523006394648e-06 NA NA NA hsa-let-7i-5p FGF11 0.0952853587923244 0.192120688957249 -0.235018569265848 0.00440939153265794 miRNATAP -0.323507607251771 2.41702652033077e-08 NA NA NA hsa-miR-1226-3p FGF11 -0.098618802853881 0.346683714981524 -0.235018569265848 0.00440939153265794 mirMAP -0.215873498479599 8.3092578295701e-12 NA NA NA hsa-miR-125a-3p FGF11 0.183834985432376 0.106230647107917 -0.235018569265848 0.00440939153265794 miRanda -0.220387739616411 4.2677254499296e-14 NA NA NA hsa-miR-125a-5p FGF11 -0.0833349424517387 0.325440200253607 -0.235018569265848 0.00440939153265794 mirMAP -0.399141179421754 4.42388956486495e-17 NA NA NA hsa-miR-125b-5p FGF11 -0.0119698382910496 0.890233923197358 -0.235018569265848 0.00440939153265794 mirMAP -0.300364716646036 2.460478659499e-07 NA NA NA hsa-miR-130b-5p FGF11 0.413070888963592 1.98866876365509e-06 -0.235018569265848 0.00440939153265794 mirMAP -0.129307922513196 0.00115639283572434 NA NA NA hsa-miR-15b-3p FGF11 0.690804403863844 8.48784755123775e-09 -0.235018569265848 0.00440939153265794 MirTarget -0.238742383514492 2.59789395249708e-19 NA NA NA hsa-miR-16-5p FGF11 0.246024668671282 0.00346042804503716 -0.235018569265848 0.00440939153265794 mirMAP -0.253885121824312 5.44203675685023e-08 NA NA NA hsa-miR-181a-5p FGF11 -0.178215604431845 0.0700010802255601 -0.235018569265848 0.00440939153265794 mirMAP -0.121480612085174 0.00953424365553838 NA NA NA hsa-miR-181b-5p FGF11 0.0260899284211646 0.768941836912518 -0.235018569265848 0.00440939153265794 mirMAP -0.216091901390167 4.37071492449882e-06 NA NA NA hsa-miR-193b-3p FGF11 0.0766333116224072 0.324723824453143 -0.235018569265848 0.00440939153265794 miRNAWalker2_validate -0.163790513828313 0.000308059901870561 NA NA NA hsa-miR-199a-5p FGF11 0.174889610847968 0.0900027418912454 -0.235018569265848 0.00440939153265794 miRanda -0.123545036604956 0.000174808908676321 NA NA NA hsa-miR-199b-5p FGF11 0.436298593972862 0.00200225454383871 -0.235018569265848 0.00440939153265794 miRanda -0.16382095505275 1.58231259921081e-12 NA NA NA hsa-miR-2355-5p FGF11 0.00808596988211185 0.916165501155096 -0.235018569265848 0.00440939153265794 mirMAP -0.404493707556144 9.44226447177025e-20 NA NA NA hsa-miR-28-5p FGF11 0.104003572105781 0.153594705579534 -0.235018569265848 0.00440939153265794 miRanda -0.300015659036983 9.49162856500363e-10 NA NA NA hsa-miR-30c-1-3p FGF11 0.0175008295781193 0.849381871022214 -0.235018569265848 0.00440939153265794 MirTarget -0.231888561690859 2.21641993413236e-10 NA NA NA hsa-miR-3127-5p FGF11 -0.0645479321229978 0.382330591204685 -0.235018569265848 0.00440939153265794 MirTarget -0.338870271012372 5.37567519810833e-14 NA NA NA hsa-miR-320c FGF11 0.343259124189966 0.000248338401291051 -0.235018569265848 0.00440939153265794 miRanda -0.104283413045425 0.00462086226364477 NA NA NA hsa-miR-324-5p FGF11 0.0183370482495953 0.842996782677271 -0.235018569265848 0.00440939153265794 miRanda -0.36724232915858 4.00522299245935e-25 NA NA NA hsa-miR-330-5p FGF11 -0.262932095995605 0.0465226565966658 -0.235018569265848 0.00440939153265794 MirTarget;PITA;miRanda;miRNATAP -0.195003196729419 3.80014150378446e-15 NA NA NA hsa-miR-331-3p FGF11 0.0126984726684189 0.905175053956974 -0.235018569265848 0.00440939153265794 miRNATAP -0.248282800419238 1.47164026963049e-15 NA NA NA hsa-miR-335-5p FGF11 0.0938511632756978 0.336889056387694 -0.235018569265848 0.00440939153265794 miRNAWalker2_validate;mirMAP -0.102652960252214 0.00341885696268571 NA NA NA hsa-miR-339-5p FGF11 0.273253751377152 0.0185300170790638 -0.235018569265848 0.00440939153265794 miRanda -0.192448682459769 1.30310049315034e-11 NA NA NA hsa-miR-432-5p FGF11 -0.214242438141977 0.166186087759946 -0.235018569265848 0.00440939153265794 mirMAP -0.188236536467299 2.1710514664352e-19 NA NA NA hsa-miR-532-3p FGF11 0.150624483952741 0.0599635132177191 -0.235018569265848 0.00440939153265794 mirMAP -0.160139075795999 0.0002261365465316 NA NA NA hsa-miR-548b-3p FGF11 0.340474544210108 0.00224491592019745 -0.235018569265848 0.00440939153265794 mirMAP -0.256817435326662 3.55493816294849e-18 NA NA NA hsa-miR-628-5p FGF11 -0.0469793532265896 0.657420003383685 -0.235018569265848 0.00440939153265794 miRNATAP -0.294133448795464 4.10014422796776e-21 NA NA NA hsa-miR-142-5p FGF12 0.434884875454107 0.000575570345702788 -0.341174347829348 0.00108179644535172 miRNATAP -0.221343945375644 9.7510045039636e-11 NA NA NA hsa-miR-200b-3p FGF12 0.245768526504511 0.0580744465744737 -0.341174347829348 0.00108179644535172 TargetScan -0.282089673650438 6.08610087552928e-17 NA NA NA hsa-miR-217 FGF12 0.540279882451941 0.00312206944061206 -0.341174347829348 0.00108179644535172 miRanda -0.180715339357903 1.50784321290264e-14 NA NA NA hsa-miR-2355-3p FGF12 0.186207523505296 0.0525918026993012 -0.341174347829348 0.00108179644535172 MirTarget;miRNATAP -0.231026550449217 3.7230945566169e-07 NA NA NA hsa-miR-3065-5p FGF12 -0.262608140481507 0.0414570974148034 -0.341174347829348 0.00108179644535172 mirMAP -0.1013901905804 0.00321299126471714 NA NA NA hsa-miR-335-3p FGF12 0.34540584335595 0.00043848660222294 -0.341174347829348 0.00108179644535172 MirTarget -0.150595502597158 0.00121258088251763 NA NA NA hsa-miR-362-3p FGF12 0.032375035235532 0.736465332563774 -0.341174347829348 0.00108179644535172 miRanda -0.173543809213136 0.000157836462891801 NA NA NA hsa-miR-3682-3p FGF12 0.113471485204649 0.266481019608971 -0.341174347829348 0.00108179644535172 MirTarget -0.262952473970731 2.37222373386136e-09 NA NA NA hsa-miR-455-5p FGF12 0.332224239762 0.0106491358056238 -0.341174347829348 0.00108179644535172 miRanda -0.224899082272953 1.63021966242583e-11 NA NA NA hsa-miR-501-5p FGF12 0.0878951859817452 0.309663377222443 -0.341174347829348 0.00108179644535172 MirTarget;PITA -0.257820236542007 4.14825558157776e-07 NA NA NA hsa-miR-542-3p FGF12 0.0835995677900829 0.403838744494071 -0.341174347829348 0.00108179644535172 miRanda -0.215735316621216 9.2374079674621e-06 NA NA NA hsa-miR-576-5p FGF12 0.106482276040047 0.194379152564166 -0.341174347829348 0.00108179644535172 mirMAP -0.290746464930981 7.73109194139942e-08 NA NA NA hsa-miR-590-3p FGF12 0.312951515438396 0.000480455423843996 -0.341174347829348 0.00108179644535172 mirMAP -0.29979405572985 6.75200328981588e-10 NA NA NA hsa-miR-1262 FGF13 0.276259331706731 0.0259799942226561 -0.706826351179643 1.15125565809917e-06 MirTarget;PITA;miRNATAP -0.343876045827969 2.47310188991752e-11 NA NA NA hsa-miR-141-3p FGF13 0.41120561991992 0.000393344390969055 -0.706826351179643 1.15125565809917e-06 TargetScan -0.224532540308352 5.58413842670053e-05 NA NA NA hsa-miR-142-5p FGF13 0.434884875454107 0.000575570345702788 -0.706826351179643 1.15125565809917e-06 PITA -0.291867410048386 3.93193139922811e-09 NA NA NA hsa-miR-34c-5p FGF13 0.181931113688093 0.257266965617363 -0.706826351179643 1.15125565809917e-06 miRanda -0.119115228141567 0.00246321570966122 NA NA NA hsa-miR-3682-3p FGF13 0.113471485204649 0.266481019608971 -0.706826351179643 1.15125565809917e-06 MirTarget -0.336026296198332 1.42601055678693e-07 NA NA NA hsa-miR-455-5p FGF13 0.332224239762 0.0106491358056238 -0.706826351179643 1.15125565809917e-06 PITA;miRanda -0.228084495997694 2.90950842226493e-06 NA NA NA hsa-miR-542-3p FGF13 0.0835995677900829 0.403838744494071 -0.706826351179643 1.15125565809917e-06 miRanda -0.249164166181018 0.000410048353399803 NA NA NA hsa-miR-548b-3p FGF13 0.340474544210108 0.00224491592019745 -0.706826351179643 1.15125565809917e-06 PITA -0.208062810230116 0.000321083900949721 NA NA NA hsa-miR-590-3p FGF13 0.312951515438396 0.000480455423843996 -0.706826351179643 1.15125565809917e-06 miRanda -0.476448111326993 9.26782882672395e-12 NA NA NA hsa-miR-125a-3p FGF14 0.183834985432376 0.106230647107917 -0.398515744120357 0.000209763793404898 miRanda -0.18209547085274 8.59757723382675e-06 NA NA NA hsa-miR-193a-5p FGF14 0.183172152450491 0.0749251910521214 -0.398515744120357 0.000209763793404898 TargetScan -0.245243369908449 9.67804779852833e-08 NA NA NA hsa-miR-362-3p FGF14 0.032375035235532 0.736465332563774 -0.398515744120357 0.000209763793404898 miRanda -0.188865716800422 8.54829762691488e-05 NA NA NA hsa-miR-421 FGF14 -0.171573235029879 0.0518112933651029 -0.398515744120357 0.000209763793404898 miRanda -0.253745288160661 1.23339623348382e-06 NA NA NA hsa-miR-590-3p FGF14 0.312951515438396 0.000480455423843996 -0.398515744120357 0.000209763793404898 miRanda -0.301628753406604 3.20594125245951e-09 NA NA NA hsa-miR-331-3p FGF17 0.0126984726684189 0.905175053956974 -0.747209687668737 1.81064553707654e-05 mirMAP -0.337058286685929 3.52810699933431e-06 NA NA NA hsa-let-7a-3p FGF2 0.0530563276543763 0.370749834463566 -0.310412510487203 1.0531264899139e-05 mirMAP -0.213352464353633 3.01948138253891e-05 NA NA NA hsa-let-7f-1-3p FGF2 0.110713086694053 0.21484524300518 -0.310412510487203 1.0531264899139e-05 mirMAP -0.103512481979716 0.000647773517119759 NA NA NA hsa-miR-103a-3p FGF2 -0.147443193527362 0.128400914654149 -0.310412510487203 1.0531264899139e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.12655516361281 0.00679371182948832 NA NA NA hsa-miR-126-5p FGF2 0.177332403948664 0.0711675116243123 -0.310412510487203 1.0531264899139e-05 mirMAP -0.110162176340636 0.000136298645021207 NA NA NA hsa-miR-140-5p FGF2 0.065063507103468 0.380452509211773 -0.310412510487203 1.0531264899139e-05 miRNAWalker2_validate -0.185361566298031 2.74319630927397e-06 NA NA NA hsa-miR-146a-5p FGF2 0.148942451196282 0.114229072828724 -0.310412510487203 1.0531264899139e-05 mirMAP -0.146277493567259 4.31070451040202e-07 NA NA NA hsa-miR-15a-5p FGF2 0.142658985373611 0.0536219185009004 -0.310412510487203 1.0531264899139e-05 MirTarget;miRNATAP -0.207678236164206 2.5972218474549e-06 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-3p FGF2 0.690804403863844 8.48784755123775e-09 -0.310412510487203 1.0531264899139e-05 mirMAP -0.137724123749645 3.22216944518618e-10 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-5p FGF2 0.592863156692837 1.41971517354055e-10 -0.310412510487203 1.0531264899139e-05 MirTarget;miRNATAP -0.259712883212368 3.66212966170394e-18 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-16-2-3p FGF2 0.468816222075619 7.04377763166292e-05 -0.310412510487203 1.0531264899139e-05 mirMAP -0.160896949653708 5.53366478735585e-13 NA NA NA hsa-miR-16-5p FGF2 0.246024668671282 0.00346042804503716 -0.310412510487203 1.0531264899139e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.238035584676493 2.36870334343313e-10 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-181a-5p FGF2 -0.178215604431845 0.0700010802255601 -0.310412510487203 1.0531264899139e-05 mirMAP -0.123717144171581 0.00107047935591826 NA NA NA hsa-miR-181b-5p FGF2 0.0260899284211646 0.768941836912518 -0.310412510487203 1.0531264899139e-05 mirMAP -0.215729610871528 1.18634456085395e-08 NA NA NA hsa-miR-19b-1-5p FGF2 0.0686579042235509 0.332645627997485 -0.310412510487203 1.0531264899139e-05 mirMAP -0.201459709129831 1.90976689008877e-07 NA NA NA hsa-miR-20a-3p FGF2 0.102743778777997 0.19677865359751 -0.310412510487203 1.0531264899139e-05 mirMAP -0.144973090746876 2.52878071686245e-05 NA NA NA hsa-miR-335-3p FGF2 0.34540584335595 0.00043848660222294 -0.310412510487203 1.0531264899139e-05 mirMAP -0.178439766262418 2.49255926048001e-10 NA NA NA hsa-miR-335-5p FGF2 0.0938511632756978 0.336889056387694 -0.310412510487203 1.0531264899139e-05 miRNATAP -0.140124965064104 6.34697534647765e-07 NA NA NA hsa-miR-361-5p FGF2 0.0107519600643302 0.882230614095565 -0.310412510487203 1.0531264899139e-05 mirMAP -0.181723993106071 8.88544014107461e-05 NA NA NA hsa-miR-376a-5p FGF2 -0.0877544252790136 0.551096721204598 -0.310412510487203 1.0531264899139e-05 mirMAP -0.113264250236629 1.03177567762586e-09 NA NA NA hsa-miR-376c-3p FGF2 -0.0513360810290662 0.710610589791979 -0.310412510487203 1.0531264899139e-05 mirMAP -0.132449788888867 7.98306703791068e-12 NA NA NA hsa-miR-424-5p FGF2 0.25140374049676 0.00709934939774983 -0.310412510487203 1.0531264899139e-05 MirTarget;miRNATAP -0.137720507275413 5.34564200507529e-06 NA NA NA hsa-miR-450b-5p FGF2 0.270544879013992 0.00801537838499562 -0.310412510487203 1.0531264899139e-05 mirMAP -0.1181608495248 1.10414336833114e-05 NA NA NA hsa-miR-92a-3p FGF2 0.0407239381334659 0.643983393805961 -0.310412510487203 1.0531264899139e-05 miRNAWalker2_validate -0.244288015297521 5.53502580269543e-07 NA NA NA hsa-miR-93-3p FGF2 0.0778767956494004 0.272224943247293 -0.310412510487203 1.0531264899139e-05 mirMAP -0.195143626431712 9.46174309952938e-07 NA NA NA hsa-miR-320a FGF7 0.092135599636725 0.243028758989929 -0.109502517174931 0.0871753790241782 miRanda -0.189627649950838 1.75375340223191e-05 NA NA NA hsa-miR-142-3p FGF9 0.558908907203977 1.37828834702416e-05 -0.595722923032546 5.09639453585729e-05 miRanda -0.282659516489835 3.27499478623632e-08 NA NA NA hsa-miR-142-5p FGF9 0.434884875454107 0.000575570345702788 -0.595722923032546 5.09639453585729e-05 mirMAP -0.263556678906143 1.6968256051013e-07 NA NA NA hsa-miR-155-5p FGF9 0.969837380981085 5.86551495710056e-15 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.453780730649256 1.65025963862586e-20 NA NA NA hsa-miR-15a-5p FGF9 0.142658985373611 0.0536219185009004 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.275420422702668 0.00935039740726957 NA NA NA hsa-miR-15b-5p FGF9 0.592863156692837 1.41971517354055e-10 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.404611884080866 2.58313582119002e-08 NA NA NA hsa-miR-186-5p FGF9 0.0606381894130505 0.514217717592464 -0.595722923032546 5.09639453585729e-05 mirMAP -0.250823884564551 0.00106977407353773 NA NA NA hsa-miR-195-3p FGF9 0.100105455903079 0.133362389112338 -0.595722923032546 5.09639453585729e-05 mirMAP;miRNATAP -0.574940764738145 5.84660729823155e-09 NA NA NA hsa-miR-195-5p FGF9 -0.00728332132631593 0.92969850577609 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.24382643607404 0.00409105332090611 NA NA NA hsa-miR-30a-3p FGF9 0.0655410309550977 0.509541256241954 -0.595722923032546 5.09639453585729e-05 mirMAP -0.555169048587157 2.90091683073997e-11 NA NA NA hsa-miR-30d-3p FGF9 0.0854049347792898 0.20691254801121 -0.595722923032546 5.09639453585729e-05 mirMAP -0.289322511444801 0.00271338910608715 NA NA NA hsa-miR-30e-3p FGF9 0.00173769765874887 0.984965501547409 -0.595722923032546 5.09639453585729e-05 mirMAP -0.277338182293572 0.00386198649418057 NA NA NA hsa-miR-320a FGF9 0.092135599636725 0.243028758989929 -0.595722923032546 5.09639453585729e-05 mirMAP;miRNATAP -0.59157746030768 5.35123905548572e-09 NA NA NA hsa-miR-320b FGF9 0.283477746304177 0.0005953763020907 -0.595722923032546 5.09639453585729e-05 mirMAP;miRNATAP -0.390562222089067 4.68249121043252e-07 NA NA NA hsa-miR-320c FGF9 0.343259124189966 0.000248338401291051 -0.595722923032546 5.09639453585729e-05 mirMAP;miRNATAP -0.476602977693011 7.45109251212317e-12 NA NA NA hsa-miR-374a-5p FGF9 0.0121037060909277 0.865417963345881 -0.595722923032546 5.09639453585729e-05 mirMAP -0.347418722893837 0.000370519855560241 NA NA NA hsa-miR-424-5p FGF9 0.25140374049676 0.00709934939774983 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.323788407456984 7.04548719212049e-06 NA NA NA hsa-miR-450b-5p FGF9 0.270544879013992 0.00801537838499562 -0.595722923032546 5.09639453585729e-05 mirMAP;miRNATAP -0.170139909599057 0.00822509033990335 NA NA NA hsa-miR-455-5p FGF9 0.332224239762 0.0106491358056238 -0.595722923032546 5.09639453585729e-05 miRanda -0.20501240822684 3.46032555683183e-05 NA NA NA hsa-miR-500a-5p FGF9 0.0634452729978863 0.396072382601677 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.343027613737247 7.65451782761426e-05 NA NA NA hsa-miR-501-3p FGF9 0.0726267165568464 0.382729320534971 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.362505345443718 6.76645261427266e-06 NA NA NA hsa-miR-502-3p FGF9 0.0276791089364088 0.697605011368848 -0.595722923032546 5.09639453585729e-05 miRNATAP -0.337427005191369 0.000251687937385934 NA NA NA hsa-miR-548b-3p FGF9 0.340474544210108 0.00224491592019745 -0.595722923032546 5.09639453585729e-05 PITA;miRNATAP -0.216483740417717 0.000218504203688734 NA NA NA hsa-miR-590-3p FGF9 0.312951515438396 0.000480455423843996 -0.595722923032546 5.09639453585729e-05 miRanda;mirMAP -0.361438804031195 4.29572281799449e-07 NA NA NA hsa-miR-590-5p FGF9 0.16946027035949 0.0386004382081678 -0.595722923032546 5.09639453585729e-05 miRanda -0.321536756569473 5.33163773528782e-05 NA NA NA hsa-miR-106b-5p FGFR1 0.167096183547615 0.0497544396383451 0.192963212582154 0.0114476358485445 mirMAP -0.131235051348832 0.00142331370486675 NA NA NA hsa-miR-135a-5p FGFR1 -0.122734612943241 0.196985964641886 0.192963212582154 0.0114476358485445 mirMAP -0.267076393133823 3.49512278163343e-16 NA NA NA hsa-miR-17-5p FGFR1 0.0975751369167082 0.20621110214601 0.192963212582154 0.0114476358485445 mirMAP -0.17274697976655 0.000506163795302763 NA NA NA hsa-miR-20a-5p FGFR1 -0.0115865752288853 0.887069448776018 0.192963212582154 0.0114476358485445 mirMAP -0.126433668888031 0.00557452572524836 NA NA NA hsa-miR-301a-3p FGFR1 0.00956875714542704 0.916380325220259 0.192963212582154 0.0114476358485445 mirMAP -0.276045190623239 1.48240939059687e-15 NA NA NA hsa-miR-3065-5p FGFR1 -0.262608140481507 0.0414570974148034 0.192963212582154 0.0114476358485445 mirMAP -0.16049264076124 1.15527531166031e-11 NA NA NA hsa-miR-19b-1-5p FGFR2 0.0686579042235509 0.332645627997485 -0.492577222868217 7.16897440371588e-05 miRNAWalker2_validate;miRTarBase -0.219237513523795 0.00418312265430993 NA NA NA hsa-miR-767-3p FGFR2 -0.429854368420513 0.000821445291167688 -0.492577222868217 7.16897440371588e-05 miRNATAP -0.194318397286558 3.97094001404658e-06 NA NA NA hsa-miR-19a-3p FGFR3 0.0466707843731911 0.576818938277755 -0.365042106979193 0.0162579686859603 mirMAP -0.403607035817958 7.63620881302308e-07 NA NA NA hsa-miR-19b-3p FGFR3 -0.0138427450847924 0.856506310673067 -0.365042106979193 0.0162579686859603 mirMAP -0.493984491016398 3.76125674952932e-07 NA NA NA hsa-miR-423-5p FGFR3 0.0965587900813789 0.148039180704338 -0.365042106979193 0.0162579686859603 MirTarget -0.461369327046826 2.91582172960651e-05 NA NA NA hsa-miR-326 FGFR4 -0.631050656251074 8.83643450601471e-05 -0.113755549365812 0.272566172396588 miRanda;miRNATAP -0.114644354916499 3.71049779113221e-05 NA NA NA hsa-miR-330-5p FGFR4 -0.262932095995605 0.0465226565966658 -0.113755549365812 0.272566172396588 miRanda;miRNATAP -0.162111818201426 2.05395711443048e-06 NA NA NA hsa-miR-491-5p FGFR4 -0.749881113074983 8.22859263179194e-09 -0.113755549365812 0.272566172396588 miRanda -0.122513697865613 0.000325785696471828 NA NA NA hsa-miR-200c-3p FLT1 0.413752622631695 8.6936333715621e-06 -0.0319580376334354 0.638676444552897 miRNAWalker2_validate;miRTarBase;MirTarget -0.106974186068777 0.000164134588758016 NA NA NA hsa-miR-93-5p FLT1 0.139766237971122 0.101057320076629 -0.0319580376334354 0.638676444552897 MirTarget;miRNATAP -0.237915238868615 6.25267586077392e-07 NA NA NA hsa-miR-491-5p FLT3LG -0.749881113074983 8.22859263179194e-09 0.250297199383235 0.00163133237530921 miRanda -0.19120255383897 4.59275417136434e-14 NA NA NA hsa-miR-326 FLT4 -0.631050656251074 8.83643450601471e-05 0.554297297147832 5.7853410758179e-11 mirMAP -0.112312658890302 7.44447214226844e-07 NA NA NA hsa-miR-331-3p FLT4 0.0126984726684189 0.905175053956974 0.554297297147832 5.7853410758179e-11 miRNAWalker2_validate -0.107717555222326 0.0023064210022874 NA NA NA hsa-miR-374a-5p FLT4 0.0121037060909277 0.865417963345881 0.554297297147832 5.7853410758179e-11 mirMAP -0.146783413937602 0.00909737149679747 NA NA NA hsa-miR-374b-5p FLT4 -0.210113949579608 0.0056866083818614 0.554297297147832 5.7853410758179e-11 mirMAP -0.179968740329611 0.00118087660409241 NA NA NA hsa-miR-491-5p FLT4 -0.749881113074983 8.22859263179194e-09 0.554297297147832 5.7853410758179e-11 miRanda -0.207101502868985 3.6785195251628e-14 NA NA NA hsa-miR-139-5p FN1 -0.647754756897633 2.15378307237163e-06 0.611643203379539 4.13939447374756e-09 miRanda -0.251483354032568 4.44995418646961e-16 NA NA NA hsa-miR-9-3p FN1 -0.184945845984695 0.0290051908009009 0.611643203379539 4.13939447374756e-09 MirTarget -0.423766015725708 2.33822633132945e-10 NA NA NA hsa-let-7a-3p FOXO3 0.0530563276543763 0.370749834463566 -0.0965102631711607 0.0758503619186141 mirMAP;miRNATAP -0.122151152187422 0.000365222677041579 NA NA NA hsa-miR-103a-3p FOXO3 -0.147443193527362 0.128400914654149 -0.0965102631711607 0.0758503619186141 mirMAP -0.145785504397847 2.65755929153254e-06 NA NA NA hsa-miR-155-5p FOXO3 0.969837380981085 5.86551495710056e-15 -0.0965102631711607 0.0758503619186141 miRNAWalker2_validate;miRTarBase -0.102270628954626 2.17212734500492e-13 27596294;25480585;20371610;26398931;22027557;27107135 MiR-155 targets 3´UTR region of multiple components of the pro-oncogenic signaling cascades including FOXO3a tumor suppressor and RUNX2 transcription factor regulating metastatic potential in BC;The effect of miRNA155 on FOXO3a expression was examined by Western blotting; FOXO3a was found to be a possible target of miRNA155 as suggested by Targetscan website;MicroRNA 155 regulates cell survival growth and chemosensitivity by targeting FOXO3a in breast cancer; In this study we demonstrate the critical role of miR-155 in regulation of cell survival and chemosensitivity through down-regulation of FOXO3a in breast cancer; Further we identified FOXO3a as a direct target of miR-155; Sustained overexpression of miR-155 resulted in repression of FOXO3a protein without changing mRNA levels and knockdown of miR-155 increases FOXO3a; Finally inverse correlation between miR-155 and FOXO3a levels were observed in a panel of breast cancer cell lines and tumors; In conclusion our study reveals a molecular link between miR-155 and FOXO3a and presents evidence that miR-155 is a critical therapeutic target in breast cancer;In contrast downregulation of miR-155-5p increased sensitivity to bufalin and upregulated the expression of FOXO3A;Here we show that hypoxic conditions induce miR-155 expression in lung cancer cells and trigger a corresponding decrease in a validated target FOXO3A;Our results further revealed that forkhead box O3a FoxO3a was a miR-155 target in the heart; And miR-155 directly repressed FoxO3a whose expression was mitigated in miR-155 agomir and mimic treatment in vivo and in vitro ;;poor survival;;; breast cancer;lymphoma;breast cancer;breast cancer;lung cancer;breast cancer hsa-miR-28-3p FOXO3 0.139444423971018 0.0811187936164314 -0.0965102631711607 0.0758503619186141 PITA;miRNATAP -0.155472959888243 9.97841467053127e-08 NA NA NA hsa-miR-28-5p FOXO3 0.104003572105781 0.153594705579534 -0.0965102631711607 0.0758503619186141 PITA;miRNATAP -0.132030489720363 7.45578418904811e-07 NA NA NA hsa-miR-30c-5p FOXO3 -0.0469114469943097 0.565934557464627 -0.0965102631711607 0.0758503619186141 miRNATAP -0.182512994651858 1.03126466885321e-10 NA NA NA hsa-miR-454-3p FOXO3 0.177318549827649 0.0120865170334418 -0.0965102631711607 0.0758503619186141 mirMAP -0.104600952788354 9.27715128064178e-05 NA NA NA hsa-miR-576-5p FOXO3 0.106482276040047 0.194379152564166 -0.0965102631711607 0.0758503619186141 mirMAP -0.148424276210835 1.73116398995034e-11 NA NA NA hsa-miR-590-3p FOXO3 0.312951515438396 0.000480455423843996 -0.0965102631711607 0.0758503619186141 PITA;miRanda;mirMAP;miRNATAP -0.116701790146842 5.36448374436433e-09 NA NA NA hsa-miR-664a-3p FOXO3 0.0413918462467198 0.611334357897022 -0.0965102631711607 0.0758503619186141 mirMAP -0.141131922829053 4.23452693321736e-10 NA NA NA hsa-miR-107 G6PC2 -0.121070851008387 0.198381281894242 -0.498423049876554 0.0208554930752471 miRanda -0.34546706109292 0.00115154185864791 NA NA NA hsa-miR-142-5p G6PC2 0.434884875454107 0.000575570345702788 -0.498423049876554 0.0208554930752471 MirTarget -0.574776962494366 1.98466834543385e-15 NA NA NA hsa-miR-193a-3p G6PC2 0.395602305834739 0.00182611787714301 -0.498423049876554 0.0208554930752471 PITA;miRanda -0.570836086928478 1.31658139419717e-14 NA NA NA hsa-miR-361-5p G6PC2 0.0107519600643302 0.882230614095565 -0.498423049876554 0.0208554930752471 miRanda -1.00109297549094 3.38201961956871e-10 NA NA NA hsa-miR-421 G6PC2 -0.171573235029879 0.0518112933651029 -0.498423049876554 0.0208554930752471 miRanda -0.846306288132763 6.15111669134805e-16 NA NA NA hsa-miR-425-5p G6PC2 -0.0412837711174561 0.682284485592858 -0.498423049876554 0.0208554930752471 MirTarget -0.543308181826414 3.28734964361486e-08 NA NA NA hsa-miR-455-5p G6PC2 0.332224239762 0.0106491358056238 -0.498423049876554 0.0208554930752471 miRanda -0.764560934866465 1.08930781526622e-28 NA NA NA hsa-miR-590-3p G6PC2 0.312951515438396 0.000480455423843996 -0.498423049876554 0.0208554930752471 miRanda -0.80363153499876 4.98627834022376e-15 NA NA NA hsa-miR-128-3p G6PC3 -0.781932174412672 7.1812756321342e-10 0.13705174763452 0.00609220452977472 miRNAWalker2_validate -0.120817700025751 7.09239642225303e-20 NA NA NA hsa-miR-15a-5p GHR 0.142658985373611 0.0536219185009004 -0.170267272957687 0.064035899095635 MirTarget;miRNATAP -0.283525052226473 1.23937238408672e-05 NA NA NA hsa-miR-15b-5p GHR 0.592863156692837 1.41971517354055e-10 -0.170267272957687 0.064035899095635 MirTarget;miRNATAP -0.167949477261025 0.000197060848540285 NA NA NA hsa-miR-16-5p GHR 0.246024668671282 0.00346042804503716 -0.170267272957687 0.064035899095635 MirTarget;miRNATAP -0.219309642371149 8.32214570465822e-05 NA NA NA hsa-miR-148b-5p GNB3 0.127111784487801 0.188731110216917 -0.200475968444105 0.0980037569221162 mirMAP -0.170907126929864 0.00205527892851483 NA NA NA hsa-miR-185-5p GNB3 -0.0767432095218297 0.404113540276816 -0.200475968444105 0.0980037569221162 MirTarget -0.350951449378987 5.0980376157585e-09 NA NA NA hsa-miR-320c GNB3 0.343259124189966 0.000248338401291051 -0.200475968444105 0.0980037569221162 miRanda -0.16943532624124 0.00344938213267382 NA NA NA hsa-miR-326 GNB3 -0.631050656251074 8.83643450601471e-05 -0.200475968444105 0.0980037569221162 miRanda -0.204318288235424 1.74539083197983e-10 NA NA NA hsa-miR-330-5p GNB3 -0.262932095995605 0.0465226565966658 -0.200475968444105 0.0980037569221162 miRanda -0.291053632628046 1.06919259567426e-13 NA NA NA hsa-miR-3622a-3p GNB3 0.0389203848593471 0.75605812962459 -0.200475968444105 0.0980037569221162 MirTarget -0.186856804314403 1.29452494634347e-05 NA NA NA hsa-miR-491-5p GNB3 -0.749881113074983 8.22859263179194e-09 -0.200475968444105 0.0980037569221162 miRanda -0.195806239369304 7.10351809231669e-07 NA NA NA hsa-miR-124-3p GNB4 -0.506026514549291 0.0078056370358648 0.153297566354816 0.0400115629824281 miRNAWalker2_validate -0.131140955047842 6.75556833257927e-21 NA NA NA hsa-miR-126-5p GNB4 0.177332403948664 0.0711675116243123 0.153297566354816 0.0400115629824281 mirMAP -0.121210035252956 3.40200062231311e-05 NA NA NA hsa-miR-133a-3p GNB4 -0.335058908302964 0.0180402783121034 0.153297566354816 0.0400115629824281 MirTarget -0.169885848469042 7.95593247928864e-20 NA NA NA hsa-miR-140-3p GNB4 -0.123864258867705 0.14796686619449 0.153297566354816 0.0400115629824281 MirTarget -0.19467097315135 4.30818273896369e-06 NA NA NA hsa-miR-203a-3p GNB4 -0.393041310603753 0.0191851699904077 0.153297566354816 0.0400115629824281 MirTarget -0.153078007818523 1.69329606169838e-22 NA NA NA hsa-miR-22-3p GNB4 0.0277287593466351 0.809325106832648 0.153297566354816 0.0400115629824281 MirTarget -0.101055336198689 0.00234098975763222 NA NA NA hsa-miR-29a-3p GNB4 -0.224140851576083 0.0349516103550236 0.153297566354816 0.0400115629824281 MirTarget -0.182379972650402 5.26860205788969e-08 NA NA NA hsa-miR-29b-3p GNB4 -0.178522712044497 0.0856106432165465 0.153297566354816 0.0400115629824281 MirTarget -0.194183700087458 2.96384607574358e-11 NA NA NA hsa-miR-29c-3p GNB4 -0.372180390387259 0.00202634431973208 0.153297566354816 0.0400115629824281 miRNAWalker2_validate;MirTarget -0.192278267049938 4.04738685118877e-15 NA NA NA hsa-miR-376a-5p GNB4 -0.0877544252790136 0.551096721204598 0.153297566354816 0.0400115629824281 MirTarget -0.110374501495641 4.66722750951084e-09 NA NA NA hsa-miR-433-3p GNB4 -0.584416505057956 0.00091765848343232 0.153297566354816 0.0400115629824281 MirTarget -0.128530289019997 1.30123303986383e-17 NA NA NA hsa-miR-582-5p GNB4 -0.215764335500665 0.0760892640434509 0.153297566354816 0.0400115629824281 MirTarget -0.183154072041297 4.6953843858999e-17 NA NA NA hsa-miR-7-1-3p GNB4 -0.0894475316162393 0.37322359785108 0.153297566354816 0.0400115629824281 MirTarget -0.250546729361893 4.66743800449427e-21 NA NA NA hsa-miR-885-5p GNB4 -0.423614695846868 1.14213289528402e-05 0.153297566354816 0.0400115629824281 MirTarget;miRNATAP -0.241999022878104 1.09576413684103e-17 NA NA NA hsa-miR-17-5p GNB5 0.0975751369167082 0.20621110214601 -0.176987527760175 0.00848636179515386 TargetScan -0.171125811929169 7.57887177376407e-05 NA NA NA hsa-miR-26b-5p GNB5 -0.0984536538762768 0.231803769574623 -0.176987527760175 0.00848636179515386 miRNAWalker2_validate -0.15088278412361 0.000115102305086069 NA NA NA hsa-miR-27a-3p GNB5 0.303760032895792 0.00457375391189806 -0.176987527760175 0.00848636179515386 miRNAWalker2_validate -0.189689297874063 1.15859987745694e-12 NA NA NA hsa-miR-542-3p GNB5 0.0835995677900829 0.403838744494071 -0.176987527760175 0.00848636179515386 miRanda -0.147022743558303 6.85906650308223e-07 NA NA NA hsa-miR-590-3p GNB5 0.312951515438396 0.000480455423843996 -0.176987527760175 0.00848636179515386 miRanda -0.16141535470045 5.80090806605299e-08 NA NA NA hsa-miR-767-3p GNG12 -0.429854368420513 0.000821445291167688 0.604079373560736 2.54919131069303e-06 PITA -0.404877496894133 3.81038934768308e-21 NA NA NA hsa-miR-96-5p GNG12 -0.217255611810229 0.209932800103896 0.604079373560736 2.54919131069303e-06 TargetScan -0.15058114761266 3.24333049347937e-06 NA NA NA hsa-miR-23a-3p GNG2 0.334693010651106 0.00144448147063433 0.00351063454247402 0.966548731653393 miRNATAP -0.253131187007529 6.67474612570918e-13 NA NA NA hsa-miR-28-5p GNG2 0.104003572105781 0.153594705579534 0.00351063454247402 0.966548731653393 miRanda;miRNATAP -0.170279269302617 0.000515850023464009 NA NA NA hsa-miR-3065-5p GNG2 -0.262608140481507 0.0414570974148034 0.00351063454247402 0.966548731653393 mirMAP;miRNATAP -0.181512530166327 7.59037152884704e-13 NA NA NA hsa-miR-30a-3p GNG2 0.0655410309550977 0.509541256241954 0.00351063454247402 0.966548731653393 mirMAP -0.398727183054275 1.8714609324061e-21 NA NA NA hsa-miR-30a-5p GNG2 -0.062942936611238 0.513851954700252 0.00351063454247402 0.966548731653393 miRNAWalker2_validate -0.579564115517773 1.04038842793485e-26 NA NA NA hsa-miR-30e-3p GNG2 0.00173769765874887 0.984965501547409 0.00351063454247402 0.966548731653393 mirMAP -0.343798163156342 1.07805060505833e-12 NA NA NA hsa-miR-500a-5p GNG2 0.0634452729978863 0.396072382601677 0.00351063454247402 0.966548731653393 mirMAP -0.2486002427408 1.82665409646733e-08 NA NA NA hsa-miR-576-5p GNG2 0.106482276040047 0.194379152564166 0.00351063454247402 0.966548731653393 mirMAP -0.229024937823673 1.65490137171413e-08 NA NA NA hsa-miR-590-3p GNG2 0.312951515438396 0.000480455423843996 0.00351063454247402 0.966548731653393 PITA;miRanda;mirMAP;miRNATAP -0.154818384131202 2.62621267758204e-05 NA NA NA hsa-miR-590-5p GNG2 0.16946027035949 0.0386004382081678 0.00351063454247402 0.966548731653393 miRanda;miRNATAP -0.186355736940742 4.80428956994954e-06 NA NA NA hsa-miR-107 GNG4 -0.121070851008387 0.198381281894242 -0.10801241280425 0.28331338760809 miRanda -0.18035621056342 0.000112995726389046 NA NA NA hsa-miR-125b-1-3p GNG4 0.365273696139707 0.00135662312552206 -0.10801241280425 0.28331338760809 MirTarget -0.26559816361558 6.88175752276102e-14 NA NA NA hsa-miR-1262 GNG4 0.276259331706731 0.0259799942226561 -0.10801241280425 0.28331338760809 mirMAP -0.32857647294086 9.23024016629209e-24 NA NA NA hsa-miR-141-3p GNG4 0.41120561991992 0.000393344390969055 -0.10801241280425 0.28331338760809 mirMAP -0.289436691600956 3.24458620748744e-16 NA NA NA hsa-miR-142-5p GNG4 0.434884875454107 0.000575570345702788 -0.10801241280425 0.28331338760809 mirMAP -0.339582701158136 7.726707428435e-28 NA NA NA hsa-miR-193b-3p GNG4 0.0766333116224072 0.324723824453143 -0.10801241280425 0.28331338760809 mirMAP -0.19623840552173 0.000483500641926725 NA NA NA hsa-miR-199a-3p GNG4 0.41226331871076 0.000497136154474528 -0.10801241280425 0.28331338760809 mirMAP -0.229470659881092 5.47433779921279e-11 NA NA NA hsa-miR-199b-3p GNG4 0.411848361704393 0.000517815011892379 -0.10801241280425 0.28331338760809 mirMAP -0.228285015601119 6.25362988897028e-11 NA NA NA hsa-miR-200b-3p GNG4 0.245768526504511 0.0580744465744737 -0.10801241280425 0.28331338760809 mirMAP -0.323779873796933 3.05568652770626e-25 NA NA NA hsa-miR-200c-3p GNG4 0.413752622631695 8.6936333715621e-06 -0.10801241280425 0.28331338760809 mirMAP -0.418116775220357 1.48284068728311e-22 NA NA NA hsa-miR-23a-3p GNG4 0.334693010651106 0.00144448147063433 -0.10801241280425 0.28331338760809 mirMAP -0.56775723073173 1.22553214812867e-42 NA NA NA hsa-miR-23b-3p GNG4 0.0476610245860094 0.630277683372344 -0.10801241280425 0.28331338760809 mirMAP -0.395764115756776 4.16954647239402e-18 NA NA NA hsa-miR-25-3p GNG4 0.316649850160985 0.000777982468387578 -0.10801241280425 0.28331338760809 mirMAP -0.181872300717914 0.00162489919492468 NA NA NA hsa-miR-29a-5p GNG4 -0.0697727833812678 0.350621682973119 -0.10801241280425 0.28331338760809 mirMAP -0.346621546536048 6.33232844142113e-10 NA NA NA hsa-miR-3065-5p GNG4 -0.262608140481507 0.0414570974148034 -0.10801241280425 0.28331338760809 mirMAP -0.211118953557085 3.38535632197126e-11 NA NA NA hsa-miR-32-5p GNG4 0.130313603388223 0.177843055266148 -0.10801241280425 0.28331338760809 mirMAP -0.28496344709277 3.8176506885768e-11 NA NA NA hsa-miR-324-5p GNG4 0.0183370482495953 0.842996782677271 -0.10801241280425 0.28331338760809 miRanda -0.17772833694493 0.000106425272722788 NA NA NA hsa-miR-342-3p GNG4 -0.125683829770877 0.105810751640137 -0.10801241280425 0.28331338760809 mirMAP -0.256768548466673 1.76338019611017e-05 NA NA NA hsa-miR-3913-5p GNG4 0.276883251911602 0.00186113779811511 -0.10801241280425 0.28331338760809 mirMAP -0.253701623256843 4.21541529119562e-08 NA NA NA hsa-miR-452-5p GNG4 0.599327068454211 1.02031931068531e-05 -0.10801241280425 0.28331338760809 mirMAP -0.278131503324159 1.47154080757358e-21 NA NA NA hsa-miR-590-3p GNG4 0.312951515438396 0.000480455423843996 -0.10801241280425 0.28331338760809 miRanda;mirMAP -0.400189090907753 4.42671661112273e-19 NA NA NA hsa-miR-92b-3p GNG4 -0.213968504161963 0.0523921855715716 -0.10801241280425 0.28331338760809 mirMAP -0.368558767404576 4.74014015497471e-18 NA NA NA hsa-miR-183-5p GNG5 -0.122352002042746 0.484640364004636 0.50308602280951 1.62304022291004e-08 MirTarget;miRNATAP -0.135433429870383 3.55216011861587e-10 NA NA NA hsa-miR-130a-5p GNG7 0.155569168973665 0.0884246261384798 -0.0651612877109073 0.368794779724342 mirMAP -0.124661590400574 8.43949583131917e-05 NA NA NA hsa-miR-135a-5p GNG7 -0.122734612943241 0.196985964641886 -0.0651612877109073 0.368794779724342 miRNATAP -0.106279495315571 0.00062775509492372 NA NA NA hsa-miR-181a-2-3p GNG7 -0.0630409614874416 0.473088318215936 -0.0651612877109073 0.368794779724342 mirMAP -0.262707802337236 6.99930464398612e-09 NA NA NA hsa-miR-195-3p GNG7 0.100105455903079 0.133362389112338 -0.0651612877109073 0.368794779724342 miRNATAP -0.150249948488372 0.000664435947308588 NA NA NA hsa-miR-92a-3p GNG7 0.0407239381334659 0.643983393805961 -0.0651612877109073 0.368794779724342 miRNAWalker2_validate -0.166201096067161 0.00131568360363746 NA NA NA hsa-miR-135a-5p GSK3B -0.122734612943241 0.196985964641886 -0.00812669327682514 0.891858643750953 miRNATAP -0.155009045241408 4.27981971726539e-12 NA NA NA hsa-miR-23a-3p GSK3B 0.334693010651106 0.00144448147063433 -0.00812669327682514 0.891858643750953 mirMAP;miRNATAP -0.109796590797861 1.08992592703854e-06 NA NA NA hsa-miR-26a-5p GSK3B 0.033823776747294 0.726610414342369 -0.00812669327682514 0.891858643750953 miRNAWalker2_validate;miRNATAP -0.124162553965791 1.9677050059957e-06 NA NA NA hsa-miR-26b-5p GSK3B -0.0984536538762768 0.231803769574623 -0.00812669327682514 0.891858643750953 miRNATAP -0.242462216035707 2.93659997949065e-16 NA NA NA hsa-miR-27a-3p GSK3B 0.303760032895792 0.00457375391189806 -0.00812669327682514 0.891858643750953 miRNATAP -0.117289047610676 2.03194827601435e-08 NA NA NA hsa-miR-28-5p GSK3B 0.104003572105781 0.153594705579534 -0.00812669327682514 0.891858643750953 miRanda;miRNATAP -0.153581288033644 5.93445649657903e-07 NA NA NA hsa-miR-29b-3p GSK3B -0.178522712044497 0.0856106432165465 -0.00812669327682514 0.891858643750953 miRTarBase;miRNATAP -0.103024519445492 4.4831296698937e-06 NA NA NA hsa-miR-30a-3p GSK3B 0.0655410309550977 0.509541256241954 -0.00812669327682514 0.891858643750953 miRNATAP -0.169749215445873 3.72672045563906e-10 NA NA NA hsa-miR-320a GSK3B 0.092135599636725 0.243028758989929 -0.00812669327682514 0.891858643750953 miRanda;mirMAP -0.157366520986088 1.86207365140069e-06 NA NA NA hsa-miR-320c GSK3B 0.343259124189966 0.000248338401291051 -0.00812669327682514 0.891858643750953 miRanda;mirMAP -0.105871202268296 3.45649295394405e-06 NA NA NA hsa-miR-362-5p GSK3B -0.0730185557229279 0.425572997292224 -0.00812669327682514 0.891858643750953 miRNATAP -0.14675268746991 1.54588705430119e-10 NA NA NA hsa-miR-374b-5p GSK3B -0.210113949579608 0.0056866083818614 -0.00812669327682514 0.891858643750953 mirMAP -0.110240926558385 0.000413708905199806 NA NA NA hsa-miR-590-3p GSK3B 0.312951515438396 0.000480455423843996 -0.00812669327682514 0.891858643750953 miRanda;miRNATAP -0.123129209082978 1.01101816997511e-07 NA NA NA hsa-miR-664a-3p GSK3B 0.0413918462467198 0.611334357897022 -0.00812669327682514 0.891858643750953 mirMAP -0.156185915215911 2.17302109400583e-09 NA NA NA hsa-miR-708-5p GSK3B -0.00936519718301376 0.919857671524527 -0.00812669327682514 0.891858643750953 miRNATAP -0.121813780965153 5.84337104157163e-07 NA NA NA hsa-miR-99a-3p GSK3B -0.122217114840767 0.0886582098510828 -0.00812669327682514 0.891858643750953 miRNATAP -0.17367661337822 1.70824739875318e-08 NA NA NA hsa-miR-128-3p GYS1 -0.781932174412672 7.1812756321342e-10 0.163492435845435 0.00180552833388296 MirTarget -0.129040041760107 6.10761375299609e-18 NA NA NA hsa-let-7b-5p HRAS 0.036218385218298 0.719557004564478 -0.0696529935819452 0.235865730348535 miRTarBase -0.105421362575589 0.000967581673758039 20607356;20881268;18344688;20033209;19323605 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells;On the basis of these data we suggest that the downregulation of let-7b and let-7e targeting K-ras and the upregulation of miR-17* a CRC marker could be considered as candidate molecular markers of cetuximab resistance;Using an established orthotopic mouse lung cancer model we show that intranasal let-7 administration reduces tumor formation in vivo in the lungs of animals expressing a G12D activating mutation for the K-ras oncogene;k-Ras and c-Myc two key oncogenes in lung cancer have been found to be targeted by let-7 in vitro; The aim of the present study is to determine the effect of let-7a a member of let-7 family on the growth of lung cancer in vivo and to investigate whether let-7-induced suppression of k-Ras and c-Myc is involved in lung cancer;Because let-7 microRNA targets the K-ras oncogene we aimed to characterize let-7 expression and function in PDAC in vitro and in vivo; Restoring let-7 levels in cancer-derived cell lines strongly inhibits cell proliferation K-ras expression and mitogen-activated protein kinase activation but fails to impede tumor growth progression after intratumoral gene transfer or after implantation of Capan-1 cells stably overexpressing let-7 microRNA ;drug resistance;;;progression glioblastoma;colorectal cancer;lung cancer;lung cancer;pancreatic cancer hsa-miR-181d-5p HRAS -0.0746131973223108 0.331281641674538 -0.0696529935819452 0.235865730348535 miRNAWalker2_validate;miRTarBase -0.133860130747548 5.02157599075857e-05 NA NA NA hsa-miR-186-5p HSP90AB1 0.0606381894130505 0.514217717592464 -0.0885514763752226 0.184182622994072 miRNAWalker2_validate -0.117223900749196 7.68476203810499e-10 NA NA NA hsa-miR-28-3p HSP90AB1 0.139444423971018 0.0811187936164314 -0.0885514763752226 0.184182622994072 miRNATAP -0.157047930398403 1.69222820958691e-09 NA NA NA hsa-miR-139-5p IBSP -0.647754756897633 2.15378307237163e-06 0.972753914429725 0.0001251791733793 miRanda -0.270585646179983 0.00120323156824052 NA NA NA hsa-miR-186-5p IFNAR1 0.0606381894130505 0.514217717592464 0.0815555266823358 0.085186300841266 mirMAP -0.118345955693356 3.03240827542331e-13 NA NA NA hsa-miR-342-3p IFNAR2 -0.125683829770877 0.105810751640137 0.224904507866693 1.10783968925471e-06 miRanda -0.120674763875099 7.96590361266096e-07 NA NA NA hsa-miR-491-5p IFNAR2 -0.749881113074983 8.22859263179194e-09 0.224904507866693 1.10783968925471e-06 miRanda -0.11148417642644 2.51011244890288e-19 NA NA NA hsa-miR-320a IGF1 0.092135599636725 0.243028758989929 -0.203195580339987 0.029670632911231 miRNATAP -0.272065311188534 2.24035754570114e-05 NA NA NA hsa-miR-592 IGF1 0.0170005651576508 0.876633091134809 -0.203195580339987 0.029670632911231 mirMAP -0.113833175952146 0.00219422982453576 NA NA NA hsa-miR-9-3p IGF1 -0.184945845984695 0.0290051908009009 -0.203195580339987 0.029670632911231 MirTarget -0.387265166384702 1.83580721842057e-09 NA NA NA hsa-let-7a-2-3p IGF1R 0.295235553592458 0.00290654476250609 -0.135857700792996 0.0366015528395905 MirTarget -0.111844113337644 4.71752976588472e-06 NA NA NA hsa-let-7d-5p IGF1R 0.111345229817909 0.0872634398336815 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate;MirTarget;miRNATAP -0.141496888931225 0.00201553181843325 25287248 Focusing on the let-7d-5p/3p pair the respectively targeted IGF1R and KRAS were shown to be in a reverse relationship with expression of the respective miRNA which was confirmed in transient transfection assays using let-7d mimic or inhibitor; Targeting of KRAS by let-7d was previous reported; targeting of IGF1R by let-7d-5p was confirmed in luciferase assays in this study colon cancer hsa-let-7e-5p IGF1R 0.0471189209570344 0.657255455444655 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate;MirTarget;miRNATAP -0.114160905645784 1.70469221142321e-05 NA NA NA hsa-let-7i-5p IGF1R 0.0952853587923244 0.192120688957249 -0.135857700792996 0.0366015528395905 MirTarget;miRNATAP -0.143251608697034 0.000624830602176317 NA NA NA hsa-miR-100-5p IGF1R 0.0939087252225708 0.437623927320764 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate;miRTarBase;miRNATAP -0.111976354725609 3.28018438141415e-06 26306402 MiR 100 Inhibits Osteosarcoma Cell Proliferation Migration and Invasion and Enhances Chemosensitivity by Targeting IGFIR sarcoma hsa-miR-1226-3p IGF1R -0.098618802853881 0.346683714981524 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate -0.121153312818867 1.08191177748407e-07 NA NA NA hsa-miR-125a-5p IGF1R -0.0833349424517387 0.325440200253607 -0.135857700792996 0.0366015528395905 mirMAP -0.147556188755988 2.29881742294783e-05 NA NA NA hsa-miR-146b-5p IGF1R 0.441078059667297 0.000237619929889385 -0.135857700792996 0.0366015528395905 miRanda -0.187507715140224 1.74252880158923e-20 NA NA NA hsa-miR-150-5p IGF1R 0.0645271721519611 0.513295658828718 -0.135857700792996 0.0366015528395905 mirMAP -0.113574958431 6.39127271912064e-06 NA NA NA hsa-miR-155-5p IGF1R 0.969837380981085 5.86551495710056e-15 -0.135857700792996 0.0366015528395905 mirMAP -0.179979563546528 2.15898058291216e-23 NA NA NA hsa-miR-15a-5p IGF1R 0.142658985373611 0.0536219185009004 -0.135857700792996 0.0366015528395905 miRNATAP -0.220577661461589 1.44916039393184e-08 NA NA NA hsa-miR-15b-3p IGF1R 0.690804403863844 8.48784755123775e-09 -0.135857700792996 0.0366015528395905 mirMAP -0.111463102273951 9.95387622335059e-09 NA NA NA hsa-miR-15b-5p IGF1R 0.592863156692837 1.41971517354055e-10 -0.135857700792996 0.0366015528395905 miRNATAP -0.173396273506064 1.10991318492391e-10 NA NA NA hsa-miR-16-1-3p IGF1R 0.346674816118499 1.86447850167636e-05 -0.135857700792996 0.0366015528395905 mirMAP -0.12478499182804 2.66303693915625e-05 NA NA NA hsa-miR-16-5p IGF1R 0.246024668671282 0.00346042804503716 -0.135857700792996 0.0366015528395905 miRNATAP -0.11321519810342 0.000784203747074522 23507142 Furthermore we confirmed that IGF1R is a direct target of miR-16; In clinical specimens IGF1R levels inversely correlate with miR-16 expression; Our results provide significant clues regarding the role of miR-16 as a tumor suppressor by targeting IGF1R in OS sarcoma hsa-miR-185-5p IGF1R -0.0767432095218297 0.404113540276816 -0.135857700792996 0.0366015528395905 MirTarget -0.141974511693695 2.19850793343084e-07 NA NA NA hsa-miR-186-5p IGF1R 0.0606381894130505 0.514217717592464 -0.135857700792996 0.0366015528395905 mirMAP -0.135875158869578 1.62131352659683e-06 NA NA NA hsa-miR-193a-5p IGF1R 0.183172152450491 0.0749251910521214 -0.135857700792996 0.0366015528395905 mirMAP -0.160873095360192 1.10303591982986e-11 NA NA NA hsa-miR-204-5p IGF1R -0.288588537398065 0.113371226435822 -0.135857700792996 0.0366015528395905 mirMAP -0.105120791209469 3.07608780592501e-16 NA NA NA hsa-miR-22-5p IGF1R 0.0437233965539527 0.639996108995995 -0.135857700792996 0.0366015528395905 miRNATAP -0.233612891233152 1.67343345024034e-20 NA NA NA hsa-miR-223-3p IGF1R -0.0272428307560952 0.814395950305568 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate;miRTarBase;MirTarget -0.105624460737198 6.62170883444042e-07 NA NA NA hsa-miR-27a-3p IGF1R 0.303760032895792 0.00457375391189806 -0.135857700792996 0.0366015528395905 mirMAP -0.12913454459956 7.6944208648304e-08 NA NA NA hsa-miR-27b-3p IGF1R -0.0599187745243146 0.58208143475992 -0.135857700792996 0.0366015528395905 mirMAP -0.130230285662681 7.21485706769308e-08 NA NA NA hsa-miR-28-3p IGF1R 0.139444423971018 0.0811187936164314 -0.135857700792996 0.0366015528395905 miRNATAP -0.219347950616091 1.27643146821018e-08 NA NA NA hsa-miR-29b-1-5p IGF1R 0.191335428755774 0.00846436454790702 -0.135857700792996 0.0366015528395905 mirMAP -0.101621751277851 0.00209474462853739 NA NA NA hsa-miR-29b-2-5p IGF1R -0.26834261955727 0.00453760540520233 -0.135857700792996 0.0366015528395905 mirMAP -0.11968024513945 3.4175624103053e-06 NA NA NA hsa-miR-29b-3p IGF1R -0.178522712044497 0.0856106432165465 -0.135857700792996 0.0366015528395905 mirMAP -0.114732262376249 8.64287684380274e-06 NA NA NA hsa-miR-30a-5p IGF1R -0.062942936611238 0.513851954700252 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate;mirMAP;miRNATAP -0.321413980606243 1.22679278357007e-15 26025408 MiR 30a suppresses non small cell lung cancer progression through AKT signaling pathway by targeting IGF1R progression lung squamous cell cancer hsa-miR-30c-5p IGF1R -0.0469114469943097 0.565934557464627 -0.135857700792996 0.0366015528395905 mirMAP;miRNATAP -0.230002465858637 8.18731367001754e-10 NA NA NA hsa-miR-30e-5p IGF1R 0.0764521828640934 0.41904991629622 -0.135857700792996 0.0366015528395905 mirMAP -0.143540390056724 5.82029246607952e-05 NA NA NA hsa-miR-335-3p IGF1R 0.34540584335595 0.00043848660222294 -0.135857700792996 0.0366015528395905 MirTarget;mirMAP -0.136751359128133 4.97175707442735e-08 NA NA NA hsa-miR-339-5p IGF1R 0.273253751377152 0.0185300170790638 -0.135857700792996 0.0366015528395905 miRanda -0.153962339964182 2.74692838301299e-14 NA NA NA hsa-miR-342-3p IGF1R -0.125683829770877 0.105810751640137 -0.135857700792996 0.0366015528395905 mirMAP -0.118476444643386 0.000633663719734603 NA NA NA hsa-miR-342-5p IGF1R 0.0383784541944436 0.544128449296127 -0.135857700792996 0.0366015528395905 mirMAP -0.112524201976166 0.00391139689026399 NA NA NA hsa-miR-34a-5p IGF1R 0.143472919835524 0.211283228567057 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate -0.191685104791376 2.10748904516836e-20 NA NA NA hsa-miR-3613-5p IGF1R 0.290347525750076 0.000408393430033494 -0.135857700792996 0.0366015528395905 mirMAP;miRNATAP -0.10521570098555 0.000356259921566232 NA NA NA hsa-miR-365a-3p IGF1R -0.184222766207919 0.0292663235724171 -0.135857700792996 0.0366015528395905 MirTarget -0.171741101211553 2.51856138923681e-09 NA NA NA hsa-miR-3677-3p IGF1R 0.527366765774056 8.67571839358047e-06 -0.135857700792996 0.0366015528395905 MirTarget -0.103538913576777 1.77620312145945e-07 NA NA NA hsa-miR-374a-5p IGF1R 0.0121037060909277 0.865417963345881 -0.135857700792996 0.0366015528395905 mirMAP -0.105064579623646 0.00384548098849303 NA NA NA hsa-miR-455-5p IGF1R 0.332224239762 0.0106491358056238 -0.135857700792996 0.0366015528395905 miRNATAP -0.103928020876914 1.28483539149689e-08 NA NA NA hsa-miR-484 IGF1R -0.0190828819137732 0.802298900989652 -0.135857700792996 0.0366015528395905 miRNAWalker2_validate -0.179299177213309 9.20541708643153e-08 NA NA NA hsa-miR-500a-3p IGF1R 0.0479457078972052 0.49420307623914 -0.135857700792996 0.0366015528395905 mirMAP -0.262497002716637 3.76506049763252e-11 NA NA NA hsa-miR-532-5p IGF1R 0.0263466708637097 0.75763006039876 -0.135857700792996 0.0366015528395905 miRNATAP -0.125250941892347 0.000262963601498015 NA NA NA hsa-miR-664a-5p IGF1R 0.0825994937558296 0.264421724444089 -0.135857700792996 0.0366015528395905 mirMAP -0.11257452285757 0.000545736921772279 NA NA NA hsa-miR-671-5p IGF1R 0.379677704170736 0.000129391032677459 -0.135857700792996 0.0366015528395905 mirMAP -0.212486325599271 4.65998192495444e-20 NA NA NA hsa-miR-877-5p IGF1R -0.0775421790642756 0.423413623033696 -0.135857700792996 0.0366015528395905 MirTarget -0.13665782646676 2.66229946061684e-08 NA NA NA hsa-miR-98-5p IGF1R -0.0806971683871618 0.251777941211683 -0.135857700792996 0.0366015528395905 MirTarget -0.151288810093955 9.06306112934266e-05 NA NA NA hsa-miR-99b-5p IGF1R 0.0938366784257259 0.419406306408393 -0.135857700792996 0.0366015528395905 miRNATAP -0.173663460909332 7.58885489256862e-11 22425712 Furthermore ectopic transfection of miR-99 family members down-regulated the expression of insulin-like growth factor 1 receptor IGF1R and mechanistic target of rapamycin mTOR genes; The deregulation of miR-99 family contributes to the tumorigenesis of HNSCC in part by targeting IGF1R and mTOR signaling pathways tumorigenesis head and neck cancer hsa-miR-767-5p IGF2 -0.551653504513145 2.61109058244854e-05 0.547749272917527 5.56068746919415e-05 mirMAP -0.138842543332253 0.00240851695785419 NA NA NA hsa-miR-9-5p IGF2 -0.125377971246422 0.269896477802284 0.547749272917527 5.56068746919415e-05 mirMAP -0.764136496575201 2.64899610699679e-09 NA NA NA hsa-miR-139-5p IKBKB -0.647754756897633 2.15378307237163e-06 0.113293458902771 0.0150457519384782 miRanda -0.130032749382716 1.65206176469125e-33 NA NA NA hsa-miR-491-5p IKBKB -0.749881113074983 8.22859263179194e-09 0.113293458902771 0.0150457519384782 miRanda -0.106383336993955 5.8305133961577e-22 NA NA NA hsa-miR-127-3p IL2RA -0.164565333886312 0.2710118155902 1.05702792804828 2.61283859117689e-07 miRanda -0.211375849076243 0.000859719069504647 NA NA NA hsa-miR-139-5p IL2RB -0.647754756897633 2.15378307237163e-06 1.00440373639227 5.18213761407867e-11 miRanda -0.351231095583441 3.32986568961996e-12 NA NA NA hsa-miR-30b-3p IL2RB -0.127948682481846 0.0864252098996846 1.00440373639227 5.18213761407867e-11 mirMAP -0.292902711413923 0.00142711396240077 NA NA NA hsa-miR-491-5p IL2RG -0.749881113074983 8.22859263179194e-09 0.671166889826602 2.23955811529298e-08 miRanda -0.17634830211617 1.01369044880547e-05 NA NA NA hsa-miR-139-5p IL6 -0.647754756897633 2.15378307237163e-06 0.860210379772624 5.71029329538237e-06 miRanda -0.193255461876079 0.00224121799463508 NA NA NA hsa-miR-20a-5p IL6R -0.0115865752288853 0.887069448776018 0.0770823555036904 0.433223467256896 mirMAP -0.30663750063624 7.91033715433322e-07 NA NA NA hsa-miR-454-3p IL6R 0.177318549827649 0.0120865170334418 0.0770823555036904 0.433223467256896 mirMAP -0.209354727979356 0.000832523423811704 NA NA NA hsa-miR-129-5p IL7 -0.567133990117126 0.00116152215679936 0.684293010681995 9.78223559554558e-10 miRanda -0.107243044709145 0.00020186000418907 NA NA NA hsa-miR-338-5p IL7 -0.424003687569629 0.00246415720301323 0.684293010681995 9.78223559554558e-10 mirMAP -0.128084950134086 0.000321798570969427 NA NA NA hsa-miR-374b-5p IL7 -0.210113949579608 0.0056866083818614 0.684293010681995 9.78223559554558e-10 mirMAP -0.260518576286309 0.000418151140098164 NA NA NA hsa-miR-200b-3p IRS1 0.245768526504511 0.0580744465744737 -0.0543333140757172 0.385337823607968 TargetScan -0.121129722755098 1.35928240806566e-09 26314828 Taken together our results suggest that miR-200 may activate AKT in lung adenocarcinoma cells through a FOG2-independent mechanism involving IRS-1 lung cancer hsa-miR-2355-3p IRS1 0.186207523505296 0.0525918026993012 -0.0543333140757172 0.385337823607968 MirTarget -0.126577002860482 1.88001504645962e-06 NA NA NA hsa-miR-27a-3p IRS1 0.303760032895792 0.00457375391189806 -0.0543333140757172 0.385337823607968 MirTarget;miRNATAP -0.115916365386676 7.52588829335376e-06 NA NA NA hsa-miR-27b-3p IRS1 -0.0599187745243146 0.58208143475992 -0.0543333140757172 0.385337823607968 MirTarget;miRNATAP -0.117196922443489 6.81321095315551e-06 NA NA NA hsa-miR-30a-5p IRS1 -0.062942936611238 0.513851954700252 -0.0543333140757172 0.385337823607968 MirTarget;miRNATAP -0.190286643429156 1.54106575790443e-05 NA NA NA hsa-miR-30c-5p IRS1 -0.0469114469943097 0.565934557464627 -0.0543333140757172 0.385337823607968 MirTarget;miRNATAP -0.241089510597176 2.0237600071976e-09 NA NA NA hsa-miR-338-3p IRS1 -0.39963704679384 0.0034410643220691 -0.0543333140757172 0.385337823607968 miRanda -0.108489978179845 1.22222189165519e-07 NA NA NA hsa-miR-361-5p IRS1 0.0107519600643302 0.882230614095565 -0.0543333140757172 0.385337823607968 miRanda -0.138687361349493 0.00167719936303043 NA NA NA hsa-miR-421 IRS1 -0.171573235029879 0.0518112933651029 -0.0543333140757172 0.385337823607968 PITA;miRanda;miRNATAP -0.140999159519838 1.31428822972192e-06 NA NA NA hsa-miR-590-3p IRS1 0.312951515438396 0.000480455423843996 -0.0543333140757172 0.385337823607968 PITA;miRanda;miRNATAP -0.133345098703871 3.02609032286624e-06 NA NA NA hsa-miR-660-5p IRS1 -0.138989893523975 0.0945491063873448 -0.0543333140757172 0.385337823607968 MirTarget;miRNATAP -0.120821607209021 0.000319138339773723 NA NA NA hsa-miR-129-5p ITGA1 -0.567133990117126 0.00116152215679936 0.647609426727057 7.58957590830003e-11 miRanda -0.105557410819997 3.44533920049207e-05 NA NA NA hsa-miR-137 ITGA1 -0.924849266740909 1.546832202049e-06 0.647609426727057 7.58957590830003e-11 miRanda -0.156902711539096 9.8579447355283e-13 NA NA NA hsa-miR-3065-5p ITGA1 -0.262608140481507 0.0414570974148034 0.647609426727057 7.58957590830003e-11 MirTarget -0.112777424072827 0.000934894293819891 NA NA NA hsa-miR-346 ITGA1 -0.577786425963268 2.10883270131593e-09 0.647609426727057 7.58957590830003e-11 miRanda -0.292211767600273 4.22283605630915e-11 NA NA NA hsa-miR-7-1-3p ITGA1 -0.0894475316162393 0.37322359785108 0.647609426727057 7.58957590830003e-11 mirMAP -0.136343503743338 0.00191572415980183 NA NA NA hsa-let-7c-5p ITGA10 -0.04295851886768 0.676052842748691 0.487033492163191 1.49502483158299e-07 miRNAWalker2_validate -0.304711367750301 2.12234945962897e-08 NA NA NA hsa-miR-3622a-3p ITGA10 0.0389203848593471 0.75605812962459 0.487033492163191 1.49502483158299e-07 MirTarget;miRNATAP -0.116668251150881 0.000453462825451388 NA NA NA hsa-miR-491-3p ITGA10 -0.523374665885358 1.93479565404951e-05 0.487033492163191 1.49502483158299e-07 miRNATAP -0.113451124046633 0.000618755302422063 NA NA NA hsa-miR-491-5p ITGA10 -0.749881113074983 8.22859263179194e-09 0.487033492163191 1.49502483158299e-07 MirTarget;miRanda -0.124570226247549 4.90458217108749e-05 NA NA NA hsa-miR-29a-3p ITGA11 -0.224140851576083 0.0349516103550236 0.310807702170357 0.000323930951988683 miRNAWalker2_validate;miRTarBase -0.132083564938413 0.00452697363606133 NA NA NA hsa-miR-139-5p ITGA2 -0.647754756897633 2.15378307237163e-06 0.355153037928839 0.00304131178560687 PITA;miRanda -0.159226995505735 5.04565253757112e-05 NA NA NA hsa-miR-19b-3p ITGA2 -0.0138427450847924 0.856506310673067 0.355153037928839 0.00304131178560687 MirTarget -0.223966443470452 0.00423394175567374 NA NA NA hsa-miR-139-5p ITGA3 -0.647754756897633 2.15378307237163e-06 0.364680029117324 0.000107542743332923 PITA;miRanda -0.119533322985041 6.78867558206547e-05 NA NA NA hsa-miR-181a-5p ITGA3 -0.178215604431845 0.0700010802255601 0.364680029117324 0.000107542743332923 miRNATAP -0.181349541771157 0.00113412604422824 NA NA NA hsa-miR-346 ITGA3 -0.577786425963268 2.10883270131593e-09 0.364680029117324 0.000107542743332923 PITA;miRanda -0.142536877128611 0.000517273150687888 NA NA NA hsa-miR-767-5p ITGA3 -0.551653504513145 2.61109058244854e-05 0.364680029117324 0.000107542743332923 MirTarget -0.11373327344962 0.000224898115536165 NA NA NA hsa-miR-139-5p ITGA4 -0.647754756897633 2.15378307237163e-06 0.755915416267331 1.40145318456044e-09 miRanda -0.179600818926381 1.59391773868857e-05 NA NA NA hsa-miR-30c-5p ITGA4 -0.0469114469943097 0.565934557464627 0.755915416267331 1.40145318456044e-09 miRNAWalker2_validate -0.308862389908018 0.000423399423028689 NA NA NA hsa-miR-346 ITGA4 -0.577786425963268 2.10883270131593e-09 0.755915416267331 1.40145318456044e-09 miRanda -0.341670540848185 1.34039092511371e-09 NA NA NA hsa-miR-137 ITGA5 -0.924849266740909 1.546832202049e-06 0.793342094273252 2.60153014639864e-16 miRNATAP -0.183465049215294 3.32771770329736e-18 NA NA NA hsa-miR-326 ITGA5 -0.631050656251074 8.83643450601471e-05 0.793342094273252 2.60153014639864e-16 PITA;miRanda;miRNATAP -0.101915908431353 9.11132766300263e-05 NA NA NA hsa-miR-338-3p ITGA5 -0.39963704679384 0.0034410643220691 0.793342094273252 2.60153014639864e-16 miRanda -0.214024001607437 1.88859538954314e-10 NA NA NA hsa-miR-346 ITGA5 -0.577786425963268 2.10883270131593e-09 0.793342094273252 2.60153014639864e-16 miRanda -0.367395525220409 3.97565367761327e-18 NA NA NA hsa-miR-301a-3p ITGA8 0.00956875714542704 0.916380325220259 -0.143995970655981 0.469099212062385 mirMAP -0.782720456368751 9.06930616750791e-16 NA NA NA hsa-miR-30c-5p ITGA8 -0.0469114469943097 0.565934557464627 -0.143995970655981 0.469099212062385 mirMAP -1.05809838646611 2.9882157695508e-15 NA NA NA hsa-miR-454-3p ITGA8 0.177318549827649 0.0120865170334418 -0.143995970655981 0.469099212062385 mirMAP -0.692253190854444 5.53689312797134e-08 NA NA NA hsa-let-7a-3p ITGAV 0.0530563276543763 0.370749834463566 0.0990544191044433 0.158112078507138 MirTarget -0.226179494184483 6.5047593966468e-07 NA NA NA hsa-miR-7-1-3p ITGAV -0.0894475316162393 0.37322359785108 0.0990544191044433 0.158112078507138 mirMAP -0.110624915473632 4.68796749491555e-06 NA NA NA hsa-miR-92a-3p ITGAV 0.0407239381334659 0.643983393805961 0.0990544191044433 0.158112078507138 MirTarget;miRNATAP -0.117649981914186 0.00735494915611702 NA NA NA hsa-miR-129-5p ITGB1 -0.567133990117126 0.00116152215679936 0.371181257932731 2.75495842292235e-08 miRanda -0.102646377953306 2.01213240209488e-13 NA NA NA hsa-miR-139-5p ITGB1 -0.647754756897633 2.15378307237163e-06 0.371181257932731 2.75495842292235e-08 miRanda -0.157507949857377 8.78792578024261e-19 NA NA NA hsa-miR-9-3p ITGB1 -0.184945845984695 0.0290051908009009 0.371181257932731 2.75495842292235e-08 miRNAWalker2_validate;MirTarget;miRNATAP -0.316742979478369 1.03356062503789e-16 NA NA NA hsa-miR-139-5p ITGB3 -0.647754756897633 2.15378307237163e-06 0.895350443268113 1.2725318963024e-11 miRanda -0.336235853933377 1.03825075718253e-14 NA NA NA hsa-miR-338-3p ITGB3 -0.39963704679384 0.0034410643220691 0.895350443268113 1.2725318963024e-11 miRanda -0.26366444264679 2.00838730947514e-08 27431198 Furthermore we also identified a metastasis related protein integrin β3 ITGB3 as a novel target gene of miR-338 metastasis lung cancer hsa-miR-767-3p ITGB3 -0.429854368420513 0.000821445291167688 0.895350443268113 1.2725318963024e-11 miRNATAP -0.173462997661818 0.000221793007799183 NA NA NA hsa-miR-409-5p ITGB4 -0.116083147809552 0.438899008482743 0.261225084677422 0.0870381748790171 miRNATAP -0.149084481265304 0.000691732465212473 NA NA NA hsa-miR-1179 ITGB5 -0.117056451908751 0.310989525634765 -0.0173135223799843 0.843025832674222 mirMAP -0.138504371856984 1.35076920981542e-05 NA NA NA hsa-miR-139-5p ITGB5 -0.647754756897633 2.15378307237163e-06 -0.0173135223799843 0.843025832674222 miRanda -0.145436981829557 6.0927739548774e-08 NA NA NA hsa-miR-130a-3p ITGB8 0.163798739071237 0.031259491216363 0.0287731618011389 0.749085546410007 MirTarget;miRNATAP -0.214737677599538 4.34855544091092e-05 NA NA NA hsa-miR-130b-3p ITGB8 0.503911467685709 9.46590588950823e-08 0.0287731618011389 0.749085546410007 MirTarget;miRNATAP -0.164661737465522 1.77398788981456e-05 NA NA NA hsa-miR-139-5p ITGB8 -0.647754756897633 2.15378307237163e-06 0.0287731618011389 0.749085546410007 PITA;miRanda;miRNATAP -0.170381107731951 1.41414791437226e-10 NA NA NA hsa-miR-145-5p ITGB8 0.108948399943015 0.310032352776693 0.0287731618011389 0.749085546410007 miRNAWalker2_validate;miRTarBase;MirTarget -0.120583757443129 0.000635801513020198 NA NA NA hsa-miR-17-5p ITGB8 0.0975751369167082 0.20621110214601 0.0287731618011389 0.749085546410007 MirTarget;TargetScan;miRNATAP -0.26335044519972 4.37026376480037e-06 NA NA NA hsa-miR-181a-5p ITGB8 -0.178215604431845 0.0700010802255601 0.0287731618011389 0.749085546410007 miRNATAP -0.144738817595009 0.00377058574497364 NA NA NA hsa-miR-19a-3p ITGB8 0.0466707843731911 0.576818938277755 0.0287731618011389 0.749085546410007 MirTarget -0.244677168999749 3.7801187935649e-08 NA NA NA hsa-miR-19b-1-5p ITGB8 0.0686579042235509 0.332645627997485 0.0287731618011389 0.749085546410007 miRNAWalker2_validate -0.269394033667863 1.30111247341512e-07 NA NA NA hsa-miR-19b-3p ITGB8 -0.0138427450847924 0.856506310673067 0.0287731618011389 0.749085546410007 MirTarget -0.368443087774913 2.3484804226982e-12 NA NA NA hsa-miR-20a-5p ITGB8 -0.0115865752288853 0.887069448776018 0.0287731618011389 0.749085546410007 MirTarget;miRNATAP -0.313904536385045 1.77047680051746e-09 NA NA NA hsa-miR-338-3p ITGB8 -0.39963704679384 0.0034410643220691 0.0287731618011389 0.749085546410007 miRanda -0.119147026110385 3.20449478455922e-05 NA NA NA hsa-miR-340-3p ITGB8 -0.107892544857248 0.133625392070518 0.0287731618011389 0.749085546410007 MirTarget -0.21588873238113 4.38608930315403e-05 NA NA NA hsa-miR-582-5p ITGB8 -0.215764335500665 0.0760892640434509 0.0287731618011389 0.749085546410007 miRNATAP -0.116536676245347 6.3468804462584e-05 NA NA NA hsa-miR-106a-5p JAK1 0.110074064773897 0.13045815990621 -0.0503084531769042 0.44032587078483 MirTarget -0.12488961834165 9.2385632187374e-05 NA NA NA hsa-miR-15b-5p JAK1 0.592863156692837 1.41971517354055e-10 -0.0503084531769042 0.44032587078483 miRNAWalker2_validate -0.106224276481482 1.94553224451923e-05 NA NA NA hsa-miR-20a-5p JAK1 -0.0115865752288853 0.887069448776018 -0.0503084531769042 0.44032587078483 MirTarget -0.134393265011369 3.17535444634639e-05 NA NA NA hsa-miR-30b-5p JAK1 -0.0858101016322106 0.263243418521854 -0.0503084531769042 0.44032587078483 MirTarget -0.107920848850336 0.00180958066794483 NA NA NA hsa-let-7a-3p JAK2 0.0530563276543763 0.370749834463566 0.0814742590745103 0.105857224086 mirMAP;miRNATAP -0.140581575924282 0.000229649587953778 NA NA NA hsa-miR-149-5p KDR -0.0535821592029952 0.657713892012548 0.270090175645727 0.00117141833672443 miRNATAP -0.139518259926423 2.03714324877248e-06 NA NA NA hsa-miR-3065-3p KDR -0.261348288537884 0.0343753235107924 0.270090175645727 0.00117141833672443 miRNATAP -0.177937727091403 1.20271139230931e-09 NA NA NA hsa-let-7a-3p KIT 0.0530563276543763 0.370749834463566 -0.261513444505928 0.0224276861944585 mirMAP -0.262331289304824 0.00525211131719426 NA NA NA hsa-miR-130a-3p KIT 0.163798739071237 0.031259491216363 -0.261513444505928 0.0224276861944585 miRNATAP -0.231855422043864 0.00150277906755264 NA NA NA hsa-miR-130b-3p KIT 0.503911467685709 9.46590588950823e-08 -0.261513444505928 0.0224276861944585 miRNATAP -0.216937004051 4.59739848770853e-05 NA NA NA hsa-miR-186-5p KIT 0.0606381894130505 0.514217717592464 -0.261513444505928 0.0224276861944585 miRNAWalker2_validate -0.34743038777356 2.40438478065183e-09 NA NA NA hsa-miR-19a-3p KIT 0.0466707843731911 0.576818938277755 -0.261513444505928 0.0224276861944585 miRTarBase;miRNATAP -0.257936426733777 3.21641658437679e-05 NA NA NA hsa-miR-19b-3p KIT -0.0138427450847924 0.856506310673067 -0.261513444505928 0.0224276861944585 miRNATAP -0.279868403634775 0.000157171592661387 NA NA NA hsa-miR-20a-5p KIT -0.0115865752288853 0.887069448776018 -0.261513444505928 0.0224276861944585 miRTarBase -0.265184760437514 0.000286322485800346 NA NA NA hsa-miR-301a-3p KIT 0.00956875714542704 0.916380325220259 -0.261513444505928 0.0224276861944585 miRNATAP -0.342457770729474 1.18002595552452e-09 NA NA NA hsa-miR-3065-5p KIT -0.262608140481507 0.0414570974148034 -0.261513444505928 0.0224276861944585 miRNATAP -0.198440173399371 2.2912016162194e-07 NA NA NA hsa-miR-454-3p KIT 0.177318549827649 0.0120865170334418 -0.261513444505928 0.0224276861944585 miRNATAP -0.427120755320098 3.91470847817685e-09 NA NA NA hsa-miR-505-3p KIT 0.115486008167472 0.109833720698312 -0.261513444505928 0.0224276861944585 MirTarget;miRNATAP -0.249282789198895 0.000599642666303086 NA NA NA hsa-miR-590-3p KIT 0.312951515438396 0.000480455423843996 -0.261513444505928 0.0224276861944585 miRanda;mirMAP -0.248407335841872 6.45963700993833e-06 NA NA NA hsa-miR-130a-5p KITLG 0.155569168973665 0.0884246261384798 -0.132761163427261 0.141337162287193 mirMAP -0.13758996984404 0.00137158076152604 NA NA NA hsa-miR-17-3p KITLG 0.0217768908350733 0.801140991374649 -0.132761163427261 0.141337162287193 MirTarget -0.220462361422951 1.62122664143473e-05 NA NA NA hsa-miR-186-5p KITLG 0.0606381894130505 0.514217717592464 -0.132761163427261 0.141337162287193 mirMAP -0.121734823324206 0.00804371048343725 NA NA NA hsa-miR-19a-3p KITLG 0.0466707843731911 0.576818938277755 -0.132761163427261 0.141337162287193 mirMAP -0.244554775973164 3.67870042951592e-07 NA NA NA hsa-miR-19b-3p KITLG -0.0138427450847924 0.856506310673067 -0.132761163427261 0.141337162287193 mirMAP -0.344866032075577 1.47814962130317e-09 NA NA NA hsa-miR-450b-5p KITLG 0.270544879013992 0.00801537838499562 -0.132761163427261 0.141337162287193 MirTarget;PITA;mirMAP -0.191852673840172 5.00570483315268e-07 NA NA NA hsa-miR-9-3p KITLG -0.184945845984695 0.0290051908009009 -0.132761163427261 0.141337162287193 MirTarget -0.265584919065147 1.74791205012059e-05 NA NA NA hsa-let-7a-3p KRAS 0.0530563276543763 0.370749834463566 -0.0976184278638819 0.0473238165779176 mirMAP;miRNATAP -0.163441029301714 2.66738583745088e-07 24727325;20603437;24890702;23324806;21994416;23167843;27620744;20177422;25183481;22584434;18922928 Association study of the let 7 miRNA complementary site variant in the 3' untranslated region of the KRAS gene in stage III colon cancer NCCTG N0147 Clinical Trial; A let-7 microRNA-complementary site LCS6 polymorphism in the 3' untranslated region of the KRAS gene has been shown to disrupt let-7 binding and upregulate KRAS expression;A let 7 microRNA binding site polymorphism in 3' untranslated region of KRAS gene predicts response in wild type KRAS patients with metastatic colorectal cancer treated with cetuximab monotherapy; A polymorphism in a let-7 microRNA complementary site lcs6 in the KRAS 3' untranslated region UTR is associated with an increased cancer risk in non-small-cell lung cancer and reduced overall survival OS in oral cancers; the presence of KRAS let-7 lcs6 polymorphism was evaluated in 130 mCRC patients who were enrolled in a phase II study of cetuximab monotherapy IMCL-0144; KRAS let-7 lcs6 polymorphism was found to be related to object response rate ORR in mCRC patients whose tumors had KRASwt;Let 7 microRNA binding site polymorphism in the 3'UTR of KRAS and colorectal cancer outcome: a systematic review and meta analysis; There is a small but growing body of literature regarding the predictive utility of a Let-7 microRNA-binding-site polymorphism in the 3'-untranslated region UTR of KRAS KRAS-LCS6 for colorectal cancer outcome although the results are conflicting; A PubMed search was conducted to identify all studies reporting on KRAS let-7 microRNA-binding site polymorphism LCS6; rs61764370 and colorectal cancer outcome;The LCS6 polymorphism in the binding site of let 7 microRNA to the KRAS 3' untranslated region: its role in the efficacy of anti EGFR based therapy in metastatic colorectal cancer patients; The lethal-7 let-7 family of microRNAs regulates KRAS activity; We studied the association of the KRAS let-7 LCS6 polymorphism with the response in 100 refractory mCRC patients treated with anti-EGFR antibodies; The KRAS let-7 LCS6 polymorphism was genotyped using the BioMark system in blood and tumor DNA samples; The KRAS let-7 LCS6 G-allele showed a statistically significant association with nonresponse to anti-EGFR-based treatment: 31.9% of patients with the T/T genotype presented a complete or a partial response versus no patients with T/G or G/G genotypes P=0.004;A let 7 microRNA SNP in the KRAS 3'UTR is prognostic in early stage colorectal cancer; Recently a SNP in a lethal-7 let-7 miRNA complementary site LCS6 in the KRAS 3'untranslated region was suggested to affect survival in metastatic CRC;Let 7 miRNA binding site polymorphism in the KRAS 3'UTR; colorectal cancer screening population prevalence and influence on clinical outcome in patients with metastatic colorectal cancer treated with 5 fluorouracil and oxaliplatin +/ cetuximab; Recent studies have reported associations between a variant allele in a let-7 microRNA complementary site LCS6 within the 3'untranslated region 3'UTR of KRAS rs61764370 and clinical outcome in metastatic colorectal cancer mCRC patients receiving cetuximab;A let 7 microRNA binding site polymorphism in the KRAS 3'UTR is associated with increased risk and reduced survival for gallbladder cancer in North Indian population; The let-7 microRNAs play a key role in regulating KRAS expression and a polymorphism in 3' untranslated region rs61764370 T/G of KRAS leads to its higher expression;Genetic modulation of the Let 7 microRNA binding to KRAS 3' untranslated region and survival of metastatic colorectal cancer patients treated with salvage cetuximab irinotecan; There is increasing evidence that the Let-7 microRNA miRNA exerts an effect as a tumor suppressor by targeting the KRAS mRNA; The Let-7 complementary site LCS6 T>G variant in the KRAS 3'-untranslated region weakens Let-7 binding;A let 7 microRNA binding site polymorphism in KRAS predicts improved outcome in patients with metastatic colorectal cancer treated with salvage cetuximab/panitumumab monotherapy;High let 7a microRNA levels in KRAS mutated colorectal carcinomas may rescue anti EGFR therapy effects in patients with chemotherapy refractory metastatic disease; Preclinical and experimental data in vivo indicate that Lethal-7 Let-7 microRNA downregulates KRAS with antitumor effects in the presence of activating KRAS mutations; In 59 patients harboring KRAS mutations Let-7a levels were analyzed for association with overall survival OS and progression-free survival PFS times; An exploratory subgroup analysis was performed using the rs61764370 LCS6 T>G polymorphism that experimentally impairs Let-7 binding to KRAS mRNA; In patients with KRAS mutations Let-7a analysis may serve to identify subgroups of patients who may still benefit from EGFR inhibition and this may open up new perspectives for alternative treatment strategies;A SNP in a let 7 microRNA complementary site in the KRAS 3' untranslated region increases non small cell lung cancer risk; The purpose of this study was to identify single nucleotide polymorphisms SNP that could modify let-7 binding and to assess the effect of such SNPs on target gene regulation and risk for non-small cell lung cancer NSCLC let-7 complementary sites LCS were sequenced in the KRAS 3' untranslated region from 74 NSCLC cases to identify mutations and SNPs that correlated with NSCLC; The LCS6 variant allele in a KRAS miRANA complementary site is significantly associated with increased risk for NSCLC among moderate smokers and represents a new paradigm for let-7 miRNAs in lung cancer susceptibility staging;drug resistance;poor survival;;drug resistance;staging;poor survival;;poor survival;poor survival;;progression;poor survival; colon cancer;colorectal cancer;colorectal cancer;colorectal cancer;colorectal cancer;colorectal cancer;bladder cancer;colorectal cancer;colorectal cancer;colorectal cancer;lung squamous cell cancer hsa-miR-30b-5p KRAS -0.0858101016322106 0.263243418521854 -0.0976184278638819 0.0473238165779176 mirMAP;miRNATAP -0.131312331179891 6.91058885171462e-07 NA NA NA hsa-miR-17-5p LAMA3 0.0975751369167082 0.20621110214601 -0.327100673217928 0.00946413584299 TargetScan -0.393782118090754 8.62478299577863e-06 NA NA NA hsa-miR-217 LAMA3 0.540279882451941 0.00312206944061206 -0.327100673217928 0.00946413584299 PITA;miRanda -0.135270475140222 6.30793577085176e-06 NA NA NA hsa-miR-124-3p LAMA4 -0.506026514549291 0.0078056370358648 0.34357357739735 0.000143732452464184 miRNAWalker2_validate -0.123949006419342 4.22218511051101e-10 NA NA NA hsa-miR-129-5p LAMA4 -0.567133990117126 0.00116152215679936 0.34357357739735 0.000143732452464184 MirTarget;miRanda -0.104576097193653 2.37289060359336e-06 NA NA NA hsa-miR-338-3p LAMA4 -0.39963704679384 0.0034410643220691 0.34357357739735 0.000143732452464184 miRanda -0.219743496703228 2.4511319568882e-13 NA NA NA hsa-miR-129-5p LAMA5 -0.567133990117126 0.00116152215679936 0.0977770802487177 0.191107394925724 miRanda -0.104926803973489 7.21248312811229e-10 NA NA NA hsa-miR-496 LAMA5 -0.139833482232877 0.39597541862532 0.0977770802487177 0.191107394925724 miRanda -0.100581068201475 2.22364483727842e-08 NA NA NA hsa-miR-124-3p LAMC1 -0.506026514549291 0.0078056370358648 0.643575172906661 1.88021715964045e-13 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.109062363630864 1.34877803871189e-08 NA NA NA hsa-miR-129-5p LAMC1 -0.567133990117126 0.00116152215679936 0.643575172906661 1.88021715964045e-13 miRanda -0.137358273741041 8.7710329568346e-11 NA NA NA hsa-miR-139-5p LAMC1 -0.647754756897633 2.15378307237163e-06 0.643575172906661 1.88021715964045e-13 miRanda -0.267458058003669 6.97400330454346e-24 NA NA NA hsa-miR-153-3p LAMC1 -0.173449510253619 0.0654368045743418 0.643575172906661 1.88021715964045e-13 MirTarget -0.230289449771916 1.10315362637141e-08 NA NA NA hsa-miR-29a-3p LAMC1 -0.224140851576083 0.0349516103550236 0.643575172906661 1.88021715964045e-13 MirTarget;miRNATAP -0.138312325298085 0.00224607726447933 26052614 The identification of laminin gamma 1 LAMC1 and myeloid cell leukemia 1 MCL1 as direct targets of miR-22 and miR-29a respectively suggested a tumor-suppressive role of these miRNAs prostate cancer hsa-miR-29b-3p LAMC1 -0.178522712044497 0.0856106432165465 0.643575172906661 1.88021715964045e-13 MirTarget;miRNATAP -0.146551772207787 0.000218138367082483 NA NA NA hsa-miR-29c-3p LAMC1 -0.372180390387259 0.00202634431973208 0.643575172906661 1.88021715964045e-13 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.109879902019624 0.00105589093987951 NA NA NA hsa-miR-338-3p LAMC1 -0.39963704679384 0.0034410643220691 0.643575172906661 1.88021715964045e-13 MirTarget;miRanda -0.235233133578316 2.91064562407468e-16 NA NA NA hsa-miR-374b-5p LAMC1 -0.210113949579608 0.0056866083818614 0.643575172906661 1.88021715964045e-13 mirMAP -0.148647334100688 0.00692929122136015 NA NA NA hsa-miR-433-3p LAMC1 -0.584416505057956 0.00091765848343232 0.643575172906661 1.88021715964045e-13 MirTarget -0.124945883929686 1.02553994694002e-09 NA NA NA hsa-miR-577 LAMC1 -0.382068371859275 0.0145513192729119 0.643575172906661 1.88021715964045e-13 PITA -0.114381225200383 1.2166650377122e-06 NA NA NA hsa-miR-7-1-3p LAMC1 -0.0894475316162393 0.37322359785108 0.643575172906661 1.88021715964045e-13 mirMAP -0.144734533518801 8.5353525077051e-05 NA NA NA hsa-miR-504-5p LAMC3 -0.510726779625117 6.12122269907368e-05 0.583003575461857 1.75384021990431e-09 MirTarget -0.133721579230376 7.58819169076368e-05 NA NA NA hsa-miR-195-3p LPAR1 0.100105455903079 0.133362389112338 0.0349777593576253 0.805265397944535 MirTarget -0.289758991262214 0.00227513313877294 NA NA NA hsa-miR-143-3p LPAR2 0.289829963197683 0.0420892105534401 0.184277383974473 0.0394619462644524 miRNATAP -0.114280936079299 0.000210560780827111 NA NA NA hsa-miR-3613-5p LPAR4 0.290347525750076 0.000408393430033494 0.124296425613282 0.297537512978733 miRNATAP -0.168766332045115 0.00853833549055672 NA NA NA hsa-miR-874-3p LPAR5 -0.342846516198707 0.00227406462925841 -0.00856877812717727 0.942356811433907 MirTarget -0.125049808854134 0.00716945937911312 NA NA NA hsa-let-7b-5p MAP2K2 0.036218385218298 0.719557004564478 0.0338816015099894 0.511977780289519 miRNAWalker2_validate -0.156288464298574 5.22052349984698e-12 NA NA NA hsa-let-7a-3p MAPK1 0.0530563276543763 0.370749834463566 -0.213353498035088 0.000389157534366882 mirMAP -0.101943093753909 0.00192343923976389 NA NA NA hsa-miR-15b-5p MAPK1 0.592863156692837 1.41971517354055e-10 -0.213353498035088 0.000389157534366882 miRNAWalker2_validate -0.146191950491781 3.61739654178022e-14 NA NA NA hsa-miR-17-5p MAPK1 0.0975751369167082 0.20621110214601 -0.213353498035088 0.000389157534366882 TargetScan -0.110084866776271 7.91694183424384e-05 NA NA NA hsa-miR-19a-3p MAPK1 0.0466707843731911 0.576818938277755 -0.213353498035088 0.000389157534366882 mirMAP -0.128018489349713 2.73892272625697e-09 NA NA NA hsa-miR-19b-3p MAPK1 -0.0138427450847924 0.856506310673067 -0.213353498035088 0.000389157534366882 mirMAP -0.176138435921289 5.03823623056019e-12 NA NA NA hsa-miR-320a MAPK1 0.092135599636725 0.243028758989929 -0.213353498035088 0.000389157534366882 miRNAWalker2_validate;PITA;miRanda;miRNATAP -0.152520075612356 2.3025642938828e-08 NA NA NA hsa-miR-454-3p MAPK1 0.177318549827649 0.0120865170334418 -0.213353498035088 0.000389157534366882 mirMAP -0.118488517070993 3.52065356643667e-06 NA NA NA hsa-miR-500a-3p MAPK1 0.0479457078972052 0.49420307623914 -0.213353498035088 0.000389157534366882 mirMAP -0.108343486711378 0.000199332911985672 NA NA NA hsa-miR-320b MAPK3 0.283477746304177 0.0005953763020907 -0.216288134544279 3.58169845564714e-05 miRNAWalker2_validate -0.103417988183093 3.03681040754152e-07 NA NA NA hsa-miR-542-3p MAPK3 0.0835995677900829 0.403838744494071 -0.216288134544279 3.58169845564714e-05 miRanda -0.133723416342879 2.41125253328141e-13 NA NA NA hsa-let-7a-3p MCL1 0.0530563276543763 0.370749834463566 0.139308533735567 0.0460458083969819 MirTarget -0.152541135457457 0.000372692802619167 NA NA NA hsa-miR-139-5p MCL1 -0.647754756897633 2.15378307237163e-06 0.139308533735567 0.0460458083969819 miRanda -0.129046146202815 1.04049486522282e-14 NA NA NA hsa-miR-153-3p MCL1 -0.173449510253619 0.0654368045743418 0.139308533735567 0.0460458083969819 miRNAWalker2_validate;miRTarBase -0.173493340982891 1.95400471724804e-12 21213215;19676043 Previously we established that microRNA-153 miR-153 induces apoptosis by downregulating B-cell lymphoma 2 Bcl-2 and myeloid cell leukemia sequence 1 Mcl-1 protein expression levels in glioblastoma cell line DBTRG-05MG;Bioinformatics analysis revealed that anti-apoptosis family member B-cell lymphoma 2 Bcl-2 and myeloid cell leukemia sequence 1 Mcl-1 are potential targets of miR-153; Indeed Western blot analysis indicated that miR-153 downregulated both Bcl-2 and Mcl-1 at the protein levels; By luciferase reporter assays we further showed that miR-153 inhibited Bcl-2 and Mcl-1 expressions by directly targeting the 3'UTR regions of their respective mRNAs ; glioblastoma;B cell lymphoma hsa-miR-193b-3p MCL1 0.0766333116224072 0.324723824453143 0.139308533735567 0.0460458083969819 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.105970682093357 0.000558977278328718 26526790;25034398;21893020 miR 193b Modulates Resistance to Doxorubicin in Human Breast Cancer Cells by Downregulating MCL 1; The aim of this study was to investigate whether miR-193b mediated the resistance of breast cancer cells to doxorubicin by targeting myeloid cell leukemia-1 MCL-1; It demonstrated that miR-193b directly targeted MCL-1 3'-UTR 3'-Untranslated Regions; Further studies indicated that miR-193b sensitized MCF-7/DOXR cells to doxorubicin through a mechanism involving the downregulation of MCL-1;Expression of miR-193b and myeloid cell leukemia-1 Mcl-1 protein were assessed by Q-PCR in situ hybridization and western blot immunohistochemistry respectively; A luciferase reporter of Mcl-1 3'-UTR was used for validation as a target of miR-193b; Significant downregulation of miR-193b and a higher level of Mcl-1 were observed in HBV-positive HCC cells and tissues; The activity of Mcl-1 3'-UTR reporter was inhibited by co-transfection with miR-193b mimic;miR 193b Regulates Mcl 1 in Melanoma; Herein we demonstrate that miR-193b also down-regulates myeloid cell leukemia sequence 1 Mcl-1 in melanoma cells; In a survey of melanoma samples the level of Mcl-1 is inversely correlated with the level of miR-193b; Overexpression of miR-193b in melanoma cells represses Mcl-1 expression; Furthermore the effect of miR-193b on the expression of Mcl-1 seems to be mediated by direct interaction between miR-193b and seed and seedless pairing sequences in the 3' untranslated region of Mcl-1 mRNA; Thus this study provides evidence that miR-193b directly regulates Mcl-1 and that down-regulation of miR-193b in vivo could be an early event in melanoma progression drug resistance;;progression breast cancer;liver cancer;melanoma hsa-miR-29a-3p MCL1 -0.224140851576083 0.0349516103550236 0.139308533735567 0.0460458083969819 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.134430122291931 1.23874252760696e-06 24155920;26052614;20041405;21471522;23484699 Finally miR-29a and miR-29b targeted MCL1 mRNA in GICs and increased apoptosis;The identification of laminin gamma 1 LAMC1 and myeloid cell leukemia 1 MCL1 as direct targets of miR-22 and miR-29a respectively suggested a tumor-suppressive role of these miRNAs;Subsequent investigation characterized two antiapoptotic molecules Bcl-2 and Mcl-1 as direct targets of miR-29; Furthermore silencing of Bcl-2 and Mcl-1 phenocopied the proapoptotic effect of miR-29 whereas overexpression of these proteins attenuated the effect of miR-29;MiR 29a down regulation in ALK positive anaplastic large cell lymphomas contributes to apoptosis blockade through MCL 1 overexpression; In addition we showed that low expression of miR-29a probably through methylation repression plays an important regulatory role in MCL-1 overexpression that could promote tumor cell survival by inhibiting apoptosis; Enforced miR-29a expression was found to modulate apoptosis through inhibition of MCL-1 expression in ALCL cell lines and in a xenografted model with a concomitant tumor growth reduction;Expression of MCL 1 and microRNA 29a in extranodal NK/T cell lymphoma tissue; This study was aimed to explore the expression level and correlation of MCL-1 and miR-29a in extranodal NK/T-cell lymphoma ENKTCL tissue; Maxvision immunohistochemistry technique and real time fluorescent quantitative PCR were used to detect the expression level of MCL-1 and miR-29a in tissue of 20 patients with ENKTCL and 10 patients with proliferative lymphadenitis respectively; Correlation analysis indicated that there was significant negative correlation between miR-29a expression and MCL-1 expression r = -0.59 P = 0.016; It is concluded that miR-29a may target MCL-1 gene regulate its expression then participate in tumorigenesis and development of ENKTCL ;;;poor survival;tumorigenesis glioblastoma;prostate cancer;liver cancer;lymphoma;lymphoma hsa-miR-29b-3p MCL1 -0.178522712044497 0.0856106432165465 0.139308533735567 0.0460458083969819 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.103132847543244 2.35612101999683e-05 24155920;20041405;25472644;27063186;19850741;24992675 Finally miR-29a and miR-29b targeted MCL1 mRNA in GICs and increased apoptosis;Subsequent investigation characterized two antiapoptotic molecules Bcl-2 and Mcl-1 as direct targets of miR-29; Furthermore silencing of Bcl-2 and Mcl-1 phenocopied the proapoptotic effect of miR-29 whereas overexpression of these proteins attenuated the effect of miR-29;Moreover miR-29b inhibited the expression of MCL1 and CDK6;miR 29b and miR 198 overexpression in CD8+ T cells of renal cell carcinoma patients down modulates JAK3 and MCL 1 leading to immune dysfunction; In particular JAK3 and MCL-1 were down-regulated in patient CD8+ T cells versus their normal counterparts likely due to defective suppressor activity of miR-29b and miR-198 in RCC CD8+ T cells; Indeed specific inhibition of miR-29b or miR-198 in peripheral blood mononuclear cells PBMCs isolated from RCC patients resulted in the up-regulation of JAK3 and MCL-1 proteins and significant improvement of cell survival in vitro;A significant enrichment for apoptosis genes including MCL-1 was found among the mRNAs inversely correlated with miR-29b expression in 45 primary AML samples;BAG3 upregulates Mcl 1 through downregulation of miR 29b to induce anticancer drug resistance in ovarian cancer; Upregulation of miR-29b also reduced levels of Mcl-1 and sensitized ES2 cells to low-dose paclitaxel; BAG3 knockdown appears to downregulate expression of Mcl-1 through upregulation of miR-29b thereby increasing the chemosensitivity of ovarian clear cell carcinoma cells ;;;poor survival;;drug resistance glioblastoma;liver cancer;colorectal cancer;kidney renal cell cancer;acute myeloid leukemia;ovarian cancer hsa-miR-885-5p MCL1 -0.423614695846868 1.14213289528402e-05 0.139308533735567 0.0460458083969819 PITA -0.17146831390665 3.1630525567029e-13 NA NA NA hsa-miR-3065-5p MDM2 -0.262608140481507 0.0414570974148034 0.179468554373188 0.00090619936510014 mirMAP -0.108004752778931 9.0880493197318e-14 NA NA NA hsa-miR-374b-5p MDM2 -0.210113949579608 0.0056866083818614 0.179468554373188 0.00090619936510014 mirMAP -0.115805376151209 4.29838757263469e-05 NA NA NA hsa-miR-504-5p MDM2 -0.510726779625117 6.12122269907368e-05 0.179468554373188 0.00090619936510014 miRNAWalker2_validate -0.122605313729819 6.11646737446648e-17 NA NA NA hsa-miR-7-1-3p MDM2 -0.0894475316162393 0.37322359785108 0.179468554373188 0.00090619936510014 mirMAP -0.103468424775092 4.37531630762283e-08 NA NA NA hsa-miR-9-3p MDM2 -0.184945845984695 0.0290051908009009 0.179468554373188 0.00090619936510014 mirMAP -0.122381796737815 5.87019491428867e-05 NA NA NA hsa-miR-628-5p MTCP1 -0.0469793532265896 0.657420003383685 0.0784693255898002 0.163429774648032 PITA;miRNATAP -0.153499219150181 4.7850242001955e-11 NA NA NA hsa-miR-101-3p MYB -0.132333931567244 0.241891922291381 0.947297821751367 1.94281683296151e-11 miRNATAP -0.296454820163484 3.99499236302413e-05 NA NA NA hsa-miR-195-5p MYB -0.00728332132631593 0.92969850577609 0.947297821751367 1.94281683296151e-11 miRNATAP -0.214911059604156 0.00950146249620595 24486218;26622903 MicroRNA 195 inhibits non small cell lung cancer cell proliferation migration and invasion by targeting MYB; Using miRNA target prediction algorithms and reporter assays we demonstrated that miR-195 suppressed the expression of MYB both at the mRNA and protein level and was directly bound to the 3'untranslated region of MYB mRNA; Overexpression of MYB in NSCLC cells using an ectopic expression vector restored the decreased cell proliferation migration and invasion effects induced by miR-195; Finally we observed an inverse correlation between MYB and miR-195 in NSCLC;microRNA 195 inhibits the proliferation migration and invasion of cervical cancer cells via the inhibition of CCND2 and MYB expression; Moreover miR-195 repressed the expression of CCND2 and MYB in the HeLa cells at the mRNA and protein levels ; lung squamous cell cancer;cervical and endocervical cancer hsa-miR-3065-5p MYB -0.262608140481507 0.0414570974148034 0.947297821751367 1.94281683296151e-11 mirMAP;miRNATAP -0.27278976713288 1.93729235905608e-08 NA NA NA hsa-miR-497-5p MYB -0.000783764887886562 0.991703566618871 0.947297821751367 1.94281683296151e-11 miRNATAP -0.241289398513356 0.00737073693921512 NA NA NA hsa-let-7c-5p MYC -0.04295851886768 0.676052842748691 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.187493318710893 0.00114816000552179 22128178 MicroRNA let 7c suppresses androgen receptor expression and activity via regulation of Myc expression in prostate cancer cells prostate cancer hsa-let-7f-5p MYC 0.0508269185845673 0.62677515045793 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.234906036376788 1.38501616912732e-05 NA NA NA hsa-let-7g-5p MYC -0.0253396334608382 0.742556489443668 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.402035322238661 2.54889860814805e-08 NA NA NA hsa-miR-145-5p MYC 0.108948399943015 0.310032352776693 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.26620175812587 1.77011092539307e-10 23919393;21836381;23499891;24356567;21092188;21690566;21723890;27482839 MicroRNA 145 function as a cell growth repressor by directly targeting c Myc in human ovarian cancer; We show that miR-145 directly target the c-Myc 3'-UTR; Moreover ectopic expression of c-Myc reduces the inhibition of cell proliferation caused by miR-145 transfection; Cell cycle assay showed that up-regulation of miR-145 reduces S phase population and restoration of c-Myc can rescue this reduction; These findings indicate that miR-145 inhibits cell proliferation and promotes cell apoptosis by targeting c-Myc 3'-UTR;The ectopic expression of miR-145 showed a significant growth inhibition in both canine and human melanoma cells tested and the effect was achieved partly by suppressing c-MYC in canine melanoma LMeC and in human melanoma A2058 and Mewo cells;The ectopic expression of miR-145 inhibited the growth of DLD-1 cells by disturbing β-catenin translocation into the nucleus thereby leading to the down-regulation of LEF/TCF transcriptional target genes c-Myc and CyclinD1;miR 145 inhibits proliferation and invasion of esophageal squamous cell carcinoma in part by targeting c Myc; The impact of overexpression of miR-145 on putative target c-Myc was subsequently confirmed via Western blot; Overexpression of miR-145 significantly decreased p < 0.05 the protein level of c-Myc which has previously been identified as a direct target of miR-145; Our results demonstrate that overexpression of miR-145 inhibits tumor growth in part by targeting c-Myc;In colon cancer cells c-Myc is a confirmed direct target for miR-145; The aim of this work was to investigate the effect of miR-145 and c-Myc on proliferation of NSCLC cells using the NSCLC cell lines A549 and H23 as models; We further showed that c-Myc was a direct target for miR-145; Taken together our study results demonstrate that miR-145 inhibits proliferation of NSCLC cells through c-Myc;miR-145 delivery reduced tumor proliferation and increased apoptosis with concomitant repression of c-Myc and ERK5 as novel regulatory target of miR-145;We found a reverse-correlation between the expression of miR-145 and its target genes such as fascin-1 c-myc SMAD2/3 and IGF-1R in breast cancer cell lines and breast cancer patient tissues;Consequently the increased miR-145 induced G1 cell cycle arrest reduced tumor proliferation and increased apoptosis inhibited HCT-116 cell migration and suppressed c-MYC expressions a regulatory target of miR-145 ;;;;;;;cell migration ovarian cancer;melanoma;colon cancer;esophageal cancer;lung squamous cell cancer;colon cancer;breast cancer;colon cancer hsa-miR-24-3p MYC 0.133257261462871 0.212960921400853 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.297311537056991 1.14667789168757e-11 NA NA NA hsa-miR-26a-5p MYC 0.033823776747294 0.726610414342369 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.349840372726607 4.11115213986892e-11 21901171 Our results identify new molecular targets of c-Myc in AML and highlight miR-26a attractiveness as a therapeutic target in leukemia acute myeloid leukemia hsa-miR-335-5p MYC 0.0938511632756978 0.336889056387694 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.221233805698315 6.63171764745164e-07 NA NA NA hsa-miR-34a-5p MYC 0.143472919835524 0.211283228567057 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.469833736893329 6.08752675410229e-40 25572695;25686834;21460242;22159222;23640973;22830357;22235332 The c-Myc and CD44 were confirmed as direct targets of miR-34a in EJ cell apoptosis induced by PRE;miR 34a induces cellular senescence via modulation of telomerase activity in human hepatocellular carcinoma by targeting FoxM1/c Myc pathway;Myc mediated repression of microRNA 34a promotes high grade transformation of B cell lymphoma by dysregulation of FoxP1;MicroRNA 34a suppresses malignant transformation by targeting c Myc transcriptional complexes in human renal cell carcinoma; We investigated the functional effects of microRNA-34a miR-34a on c-Myc transcriptional complexes in renal cell carcinoma; miR-34a down-regulated expression of multiple oncogenes including c-Myc by targeting its 3' untranslated region which was revealed by luciferase reporter assays; Our results demonstrate that miR-34a suppresses assembly and function of the c-Myc complex that activates or elongates transcription indicating a novel role of miR-34a in the regulation of transcription by c-Myc;Among them miR-34a was also associated with poor prognosis in 2 independent series of leukemic and nodal MCL and in cooperation with high expression of the MYC oncogene;We report that miR-34a did not inhibit cell proliferation notwithstanding a marked down-regulation of c-MYC;MicroRNA 34a modulates c Myc transcriptional complexes to suppress malignancy in human prostate cancer cells; We studied the functional effects of miR-34a on c-Myc transcriptional complexes in PC-3 prostate cancer cells; miR-34a downregulated expression of c-Myc oncogene by targeting its 3' UTR as shown by luciferase reporter assays; This is the first report to document that miR-34a suppresses assembly and function of the c-Myc-Skp2-Miz1 complex that activates RhoA and the c-Myc-pTEFB complex that elongates transcription of various genes suggesting a novel role of miR-34a in the regulation of transcription by c-Myc complex ;;;malignant trasformation;worse prognosis;; bladder cancer;liver cancer;B cell lymphoma;kidney renal cell cancer;lymphoma;lymphoma;prostate cancer hsa-miR-34c-5p MYC 0.181931113688093 0.257266965617363 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase;miRanda -0.212118906251778 1.73484497587865e-16 NA NA NA hsa-miR-377-5p MYC -0.254102489855821 0.081507589808783 0.0342990533418108 0.742963156666191 miRNATAP -0.197215758202484 1.41992056070433e-11 NA NA NA hsa-miR-378a-3p MYC 0.251965146196782 0.0137483824840962 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.135286330402019 0.00204077080845563 NA NA NA hsa-miR-590-3p MYC 0.312951515438396 0.000480455423843996 0.0342990533418108 0.742963156666191 miRanda -0.223628987104378 2.58191493375387e-06 24200043 We identified important regulators MYC NFKB1 miR-590 and miR-570 and significant miRNA-TF synergistic regulatory motifs by random simulations lung squamous cell cancer hsa-miR-744-5p MYC -0.31868579180387 0.000969037378618306 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.208939879123018 7.28554627025748e-06 24991193 Decrease expression of microRNA 744 promotes cell proliferation by targeting c Myc in human hepatocellular carcinoma; Quantitative reverse-transcription polymerase chain reaction qRT-PCR was conducted to detect the expression of miR-744 and Immunohistochemistry was performed to detect expression of c-Myc in HCC specimens and adjacent normal tissues; Luciferase reporter assays was performed to confirm whether miR-744 regulated the expression of c-Myc; Luciferase assay and Western blot analysis revealed that c-Myc is a direct target of miR-744; Down-regulation of miR-744 and up-regulation of c-Myc were detected in HCC specimens compared with adjacent normal tissues; Moreover restoration of miR-744 rescues c-Myc induced HCC proliferation; Our data suggest that miR-744 exerts its tumor suppressor function by targeting c-Myc leading to the inhibition of HCC cell growth liver cancer hsa-miR-98-5p MYC -0.0806971683871618 0.251777941211683 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.482234879670646 1.25183084383233e-12 NA NA NA hsa-miR-1179 NFKB1 -0.117056451908751 0.310989525634765 0.146182859193676 0.0048570839713069 miRNATAP -0.140273206429428 5.35154124955416e-17 NA NA NA hsa-miR-508-3p NFKB1 -0.292045725850845 0.0158994578296519 0.146182859193676 0.0048570839713069 MirTarget;PITA;miRNATAP -0.101247627472892 1.16240035097528e-10 NA NA NA hsa-miR-9-5p NFKB1 -0.125377971246422 0.269896477802284 0.146182859193676 0.0048570839713069 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.336428914988368 4.98439106747222e-16 NA NA NA hsa-let-7c-5p NGF -0.04295851886768 0.676052842748691 -0.416457950464278 0.0030667540551424 MirTarget -0.360257350329555 1.22298479321338e-05 NA NA NA hsa-let-7e-5p NGF 0.0471189209570344 0.657255455444655 -0.416457950464278 0.0030667540551424 MirTarget -0.207654601525943 0.00237596292059546 NA NA NA hsa-let-7i-5p NGF 0.0952853587923244 0.192120688957249 -0.416457950464278 0.0030667540551424 MirTarget -0.412016019685442 0.000120893499183623 NA NA NA hsa-miR-217 NGF 0.540279882451941 0.00312206944061206 -0.416457950464278 0.0030667540551424 miRanda -0.106355636018351 0.00151830983295066 NA NA NA hsa-miR-320a NGF 0.092135599636725 0.243028758989929 -0.416457950464278 0.0030667540551424 miRanda -0.445535637234912 4.53615837617544e-06 NA NA NA hsa-miR-320b NGF 0.283477746304177 0.0005953763020907 -0.416457950464278 0.0030667540551424 miRanda -0.295144471999818 6.92199710987292e-05 NA NA NA hsa-miR-320c NGF 0.343259124189966 0.000248338401291051 -0.416457950464278 0.0030667540551424 miRanda -0.420643347773402 2.54365030935421e-10 NA NA NA hsa-miR-423-5p NGF 0.0965587900813789 0.148039180704338 -0.416457950464278 0.0030667540551424 MirTarget;miRNATAP -0.377352274624578 0.000280283896732406 NA NA NA hsa-miR-542-3p NR4A1 0.0835995677900829 0.403838744494071 0.0613075765545483 0.584024522339382 PITA;miRanda -0.147057593375991 0.00410521002686925 NA NA NA hsa-miR-29a-3p NRAS -0.224140851576083 0.0349516103550236 0.166825456686155 0.00198701675555357 miRNATAP -0.113429370178798 7.18564270439906e-07 NA NA NA hsa-miR-29c-3p NRAS -0.372180390387259 0.00202634431973208 0.166825456686155 0.00198701675555357 miRNATAP -0.103186593831709 9.57910873311229e-10 NA NA NA hsa-miR-664a-3p NRAS 0.0413918462467198 0.611334357897022 0.166825456686155 0.00198701675555357 MirTarget -0.11306016643066 1.53334474091839e-06 NA NA NA hsa-miR-137 NTRK1 -0.924849266740909 1.546832202049e-06 0.207348057458216 0.0386791072527031 miRanda -0.117432442139169 1.38166281095279e-07 NA NA NA hsa-miR-1179 NTRK2 -0.117056451908751 0.310989525634765 -0.686181111372964 1.63868289231844e-08 mirMAP -0.117785076587188 0.00455990920871272 NA NA NA hsa-miR-125a-3p NTRK2 0.183834985432376 0.106230647107917 -0.686181111372964 1.63868289231844e-08 miRanda -0.117049049301132 0.00488026725384927 NA NA NA hsa-miR-126-5p NTRK2 0.177332403948664 0.0711675116243123 -0.686181111372964 1.63868289231844e-08 mirMAP -0.210559355701016 2.22333970399353e-05 NA NA NA hsa-miR-130b-3p NTRK2 0.503911467685709 9.46590588950823e-08 -0.686181111372964 1.63868289231844e-08 mirMAP -0.48198693041308 1.47745844823795e-23 NA NA NA hsa-miR-130b-5p NTRK2 0.413070888963592 1.98866876365509e-06 -0.686181111372964 1.63868289231844e-08 mirMAP -0.348405926099145 1.78702109563048e-10 NA NA NA hsa-miR-140-3p NTRK2 -0.123864258867705 0.14796686619449 -0.686181111372964 1.63868289231844e-08 PITA -0.197847075214346 0.00628433840688937 NA NA NA hsa-miR-140-5p NTRK2 0.065063507103468 0.380452509211773 -0.686181111372964 1.63868289231844e-08 miRanda -0.259527342352345 0.000145290714682029 NA NA NA hsa-miR-143-3p NTRK2 0.289829963197683 0.0420892105534401 -0.686181111372964 1.63868289231844e-08 mirMAP -0.185965853420384 4.47219518844906e-07 NA NA NA hsa-miR-145-3p NTRK2 0.110543172922298 0.294150620210019 -0.686181111372964 1.63868289231844e-08 mirMAP -0.20443079458531 3.43520671853098e-06 NA NA NA hsa-miR-146b-5p NTRK2 0.441078059667297 0.000237619929889385 -0.686181111372964 1.63868289231844e-08 PITA;miRanda -0.285774957078472 8.72386440944543e-13 NA NA NA hsa-miR-155-5p NTRK2 0.969837380981085 5.86551495710056e-15 -0.686181111372964 1.63868289231844e-08 mirMAP -0.311414873073455 1.77395270740727e-18 NA NA NA hsa-miR-15b-3p NTRK2 0.690804403863844 8.48784755123775e-09 -0.686181111372964 1.63868289231844e-08 mirMAP -0.275120345518809 2.07845831566898e-13 NA NA NA hsa-miR-17-3p NTRK2 0.0217768908350733 0.801140991374649 -0.686181111372964 1.63868289231844e-08 miRNATAP -0.267488399217568 1.51674456927697e-05 NA NA NA hsa-miR-181b-5p NTRK2 0.0260899284211646 0.768941836912518 -0.686181111372964 1.63868289231844e-08 mirMAP -0.275460361374369 2.69131478075794e-05 NA NA NA hsa-miR-200c-3p NTRK2 0.413752622631695 8.6936333715621e-06 -0.686181111372964 1.63868289231844e-08 miRNAWalker2_validate;miRTarBase -0.189312281448708 0.000151698853560443 23209748;23074172 miR 200c sensitizes breast cancer cells to doxorubicin treatment by decreasing TrkB and Bmi1 expression;miR-200c also targets TrkB a mediator of resistance to anoikis ;drug resistance breast cancer;ovarian cancer hsa-miR-21-5p NTRK2 1.0504925175311 2.58081295984106e-11 -0.686181111372964 1.63868289231844e-08 mirMAP -0.236108791238634 4.9068404718365e-13 NA NA NA hsa-miR-27b-5p NTRK2 0.224282840351546 0.00630787296051719 -0.686181111372964 1.63868289231844e-08 mirMAP -0.182354407883954 0.00140069809613523 NA NA NA hsa-miR-320a NTRK2 0.092135599636725 0.243028758989929 -0.686181111372964 1.63868289231844e-08 mirMAP -0.320169674390035 1.45235884491897e-05 NA NA NA hsa-miR-320b NTRK2 0.283477746304177 0.0005953763020907 -0.686181111372964 1.63868289231844e-08 mirMAP -0.297676719953335 1.05142976932486e-07 NA NA NA hsa-miR-335-3p NTRK2 0.34540584335595 0.00043848660222294 -0.686181111372964 1.63868289231844e-08 mirMAP -0.301664968205129 5.38168841975694e-10 NA NA NA hsa-miR-335-5p NTRK2 0.0938511632756978 0.336889056387694 -0.686181111372964 1.63868289231844e-08 miRNAWalker2_validate -0.142160869298563 0.00359656740597952 NA NA NA hsa-miR-339-5p NTRK2 0.273253751377152 0.0185300170790638 -0.686181111372964 1.63868289231844e-08 miRanda -0.145781932800226 0.000287943821956961 NA NA NA hsa-miR-361-5p NTRK2 0.0107519600643302 0.882230614095565 -0.686181111372964 1.63868289231844e-08 miRanda;mirMAP -0.29237397328415 0.000253896813933908 NA NA NA hsa-miR-376c-3p NTRK2 -0.0513360810290662 0.710610589791979 -0.686181111372964 1.63868289231844e-08 mirMAP -0.140327427013934 3.33516406165561e-05 NA NA NA hsa-miR-409-3p NTRK2 0.00456692516634361 0.974143243758506 -0.686181111372964 1.63868289231844e-08 mirMAP -0.110267529518122 0.000884459244523279 NA NA NA hsa-miR-452-5p NTRK2 0.599327068454211 1.02031931068531e-05 -0.686181111372964 1.63868289231844e-08 mirMAP -0.169844766254734 5.09543021020336e-07 NA NA NA hsa-miR-454-3p NTRK2 0.177318549827649 0.0120865170334418 -0.686181111372964 1.63868289231844e-08 mirMAP -0.20934244817509 0.00245368136356355 NA NA NA hsa-miR-501-5p NTRK2 0.0878951859817452 0.309663377222443 -0.686181111372964 1.63868289231844e-08 mirMAP -0.145000560300687 0.00765735539714478 NA NA NA hsa-miR-590-3p NTRK2 0.312951515438396 0.000480455423843996 -0.686181111372964 1.63868289231844e-08 PITA;miRanda;mirMAP -0.221156268667124 2.02480061352176e-05 NA NA NA hsa-miR-590-5p NTRK2 0.16946027035949 0.0386004382081678 -0.686181111372964 1.63868289231844e-08 miRanda;mirMAP -0.155176251936937 0.00726049632647802 NA NA NA hsa-miR-592 OSM 0.0170005651576508 0.876633091134809 0.470952885273382 0.00656704512504158 PITA;miRNATAP -0.432777730104561 2.96495305804742e-10 NA NA NA hsa-miR-139-5p OSMR -0.647754756897633 2.15378307237163e-06 0.702146516650034 1.4597089315706e-08 miRanda -0.211914432735387 1.96756060590983e-07 NA NA NA hsa-miR-892a OSMR -0.242011750672218 0.136146245118012 0.702146516650034 1.4597089315706e-08 miRNATAP -0.116563572731247 0.000730243735238085 NA NA NA hsa-miR-3065-5p PDGFA -0.262608140481507 0.0414570974148034 0.490091510735296 1.12439737196867e-06 mirMAP -0.172968495924815 2.92745603338613e-07 NA NA NA hsa-miR-767-5p PDGFB -0.551653504513145 2.61109058244854e-05 0.264702010292632 0.000199580243175127 PITA;miRNATAP -0.139160724595735 2.76800071159541e-10 NA NA NA hsa-let-7f-1-3p PDGFC 0.110713086694053 0.21484524300518 -0.109641054681205 0.135071652654198 mirMAP -0.148071279655735 6.42277243999981e-06 NA NA NA hsa-miR-124-3p PDGFC -0.506026514549291 0.0078056370358648 -0.109641054681205 0.135071652654198 miRNAWalker2_validate -0.130716324407655 4.05635206169265e-18 NA NA NA hsa-miR-16-2-3p PDGFC 0.468816222075619 7.04377763166292e-05 -0.109641054681205 0.135071652654198 mirMAP -0.111453310676802 5.82412948921634e-06 NA NA NA hsa-miR-326 PDGFC -0.631050656251074 8.83643450601471e-05 -0.109641054681205 0.135071652654198 miRanda -0.101211829596614 1.66148480926124e-08 NA NA NA hsa-miR-330-3p PDGFC -0.427388106938151 0.00300489284414266 -0.109641054681205 0.135071652654198 mirMAP -0.132885747489891 3.05218892289238e-11 NA NA NA hsa-miR-330-5p PDGFC -0.262932095995605 0.0465226565966658 -0.109641054681205 0.135071652654198 miRanda -0.155809696055812 1.10163134717387e-12 NA NA NA hsa-miR-338-5p PDGFC -0.424003687569629 0.00246415720301323 -0.109641054681205 0.135071652654198 PITA;mirMAP -0.10503757056074 6.27789583606703e-07 NA NA NA hsa-miR-361-5p PDGFC 0.0107519600643302 0.882230614095565 -0.109641054681205 0.135071652654198 miRanda -0.224053941945922 8.20863492198512e-06 NA NA NA hsa-miR-375 PDGFC -0.0268401141324031 0.858358101444697 -0.109641054681205 0.135071652654198 miRNAWalker2_validate;miRanda -0.138081388519449 4.91964996860909e-13 NA NA NA hsa-miR-496 PDGFC -0.139833482232877 0.39597541862532 -0.109641054681205 0.135071652654198 miRanda -0.12723146771382 7.95788464975575e-13 NA NA NA hsa-miR-543 PDGFC -0.347737549779625 0.0157394585079683 -0.109641054681205 0.135071652654198 miRanda -0.157136479268045 1.37149911666354e-14 NA NA NA hsa-miR-582-3p PDGFC -0.11807589183011 0.304077332003677 -0.109641054681205 0.135071652654198 PITA -0.192828627052514 6.4461573596631e-14 NA NA NA hsa-miR-7-1-3p PDGFC -0.0894475316162393 0.37322359785108 -0.109641054681205 0.135071652654198 mirMAP -0.27684551728089 1.94909419223997e-22 NA NA NA hsa-miR-7-5p PDGFC -0.244254069762734 0.193173919522103 -0.109641054681205 0.135071652654198 miRNAWalker2_validate -0.132843663302499 1.03803908284809e-17 NA NA NA hsa-miR-346 PDGFD -0.577786425963268 2.10883270131593e-09 0.510250241810776 0.00492127208816491 miRanda -0.220480571429855 0.00717857364172508 NA NA NA hsa-miR-9-5p PDGFD -0.125377971246422 0.269896477802284 0.510250241810776 0.00492127208816491 MirTarget -1.28258540639006 6.51039308150004e-14 NA NA NA hsa-let-7a-2-3p PDGFRA 0.295235553592458 0.00290654476250609 -0.214227835759073 0.123422750860196 MirTarget -0.296334233533416 4.11922197722786e-07 NA NA NA hsa-let-7f-1-3p PDGFRA 0.110713086694053 0.21484524300518 -0.214227835759073 0.123422750860196 mirMAP -0.239111230215239 0.000205273554105078 NA NA NA hsa-miR-106a-5p PDGFRA 0.110074064773897 0.13045815990621 -0.214227835759073 0.123422750860196 MirTarget;miRNATAP -0.291101709809366 0.000522324338760065 NA NA NA hsa-miR-125a-3p PDGFRA 0.183834985432376 0.106230647107917 -0.214227835759073 0.123422750860196 miRanda -0.218704614786785 1.83272444389053e-05 NA NA NA hsa-miR-125a-5p PDGFRA -0.0833349424517387 0.325440200253607 -0.214227835759073 0.123422750860196 miRanda -0.584043237308749 1.35379706476998e-12 NA NA NA hsa-miR-132-3p PDGFRA -0.178379362833856 0.13335200877129 -0.214227835759073 0.123422750860196 miRNATAP -0.187831850273597 0.000250319391344747 NA NA NA hsa-miR-141-3p PDGFRA 0.41120561991992 0.000393344390969055 -0.214227835759073 0.123422750860196 MirTarget -0.212944157891481 2.31787320191516e-05 NA NA NA hsa-miR-142-5p PDGFRA 0.434884875454107 0.000575570345702788 -0.214227835759073 0.123422750860196 PITA;mirMAP;miRNATAP -0.255106354672294 1.29328581244106e-08 NA NA NA hsa-miR-143-3p PDGFRA 0.289829963197683 0.0420892105534401 -0.214227835759073 0.123422750860196 miRNATAP -0.188499057787187 3.61701378371554e-05 NA NA NA hsa-miR-146b-5p PDGFRA 0.441078059667297 0.000237619929889385 -0.214227835759073 0.123422750860196 miRanda -0.439250339471549 1.63797229980143e-19 NA NA NA hsa-miR-149-5p PDGFRA -0.0535821592029952 0.657713892012548 -0.214227835759073 0.123422750860196 MirTarget;miRNATAP -0.251126677334923 2.0149615804524e-07 NA NA NA hsa-miR-16-1-3p PDGFRA 0.346674816118499 1.86447850167636e-05 -0.214227835759073 0.123422750860196 MirTarget -0.225776587776573 0.0016066501712407 NA NA NA hsa-miR-184 PDGFRA -0.842534984406592 6.08844932016991e-08 -0.214227835759073 0.123422750860196 miRanda -0.153784899830709 2.69381514856598e-05 NA NA NA hsa-miR-186-5p PDGFRA 0.0606381894130505 0.514217717592464 -0.214227835759073 0.123422750860196 MirTarget -0.297882320589264 1.23111767407722e-05 NA NA NA hsa-miR-193a-3p PDGFRA 0.395602305834739 0.00182611787714301 -0.214227835759073 0.123422750860196 miRanda -0.273002234423519 2.46644840364766e-09 NA NA NA hsa-miR-199a-3p PDGFRA 0.41226331871076 0.000497136154474528 -0.214227835759073 0.123422750860196 MirTarget;PITA;miRNATAP -0.270733214461539 2.93296438648829e-08 NA NA NA hsa-miR-199a-5p PDGFRA 0.174889610847968 0.0900027418912454 -0.214227835759073 0.123422750860196 miRanda -0.26790376826415 1.965281983763e-06 NA NA NA hsa-miR-199b-3p PDGFRA 0.411848361704393 0.000517815011892379 -0.214227835759073 0.123422750860196 MirTarget;PITA;miRNATAP -0.269173280120204 3.28792716796774e-08 NA NA NA hsa-miR-199b-5p PDGFRA 0.436298593972862 0.00200225454383871 -0.214227835759073 0.123422750860196 miRanda -0.181040865656848 7.35837504952751e-06 NA NA NA hsa-miR-20b-5p PDGFRA 0.144541062989476 0.0992042333556543 -0.214227835759073 0.123422750860196 MirTarget;miRNATAP -0.280949056106333 7.10780599325753e-05 NA NA NA hsa-miR-212-3p PDGFRA -0.014112941209349 0.918360379512974 -0.214227835759073 0.123422750860196 miRNATAP -0.165210568743679 7.56823088897284e-05 NA NA NA hsa-miR-24-3p PDGFRA 0.133257261462871 0.212960921400853 -0.214227835759073 0.123422750860196 MirTarget;miRNATAP -0.258693453202796 1.426608452579e-05 NA NA NA hsa-miR-26a-5p PDGFRA 0.033823776747294 0.726610414342369 -0.214227835759073 0.123422750860196 MirTarget;mirMAP;miRNATAP -0.337817549965926 2.64891798560313e-06 NA NA NA hsa-miR-26b-5p PDGFRA -0.0984536538762768 0.231803769574623 -0.214227835759073 0.123422750860196 miRNAWalker2_validate;MirTarget;mirMAP;miRNATAP -0.330492068006605 7.86276476912204e-05 NA NA NA hsa-miR-27a-3p PDGFRA 0.303760032895792 0.00457375391189806 -0.214227835759073 0.123422750860196 MirTarget;miRNATAP -0.332717954435526 7.42608425988857e-09 NA NA NA hsa-miR-27b-3p PDGFRA -0.0599187745243146 0.58208143475992 -0.214227835759073 0.123422750860196 MirTarget;miRNATAP -0.312048975764859 7.61491715727402e-08 NA NA NA hsa-miR-28-5p PDGFRA 0.104003572105781 0.153594705579534 -0.214227835759073 0.123422750860196 miRanda -0.408002281031611 1.50274553396473e-06 NA NA NA hsa-miR-29a-3p PDGFRA -0.224140851576083 0.0349516103550236 -0.214227835759073 0.123422750860196 MirTarget -0.433860748502012 5.47540666642455e-10 NA NA NA hsa-miR-29b-1-5p PDGFRA 0.191335428755774 0.00846436454790702 -0.214227835759073 0.123422750860196 mirMAP -0.444219655383209 1.48743564059219e-08 NA NA NA hsa-miR-29b-2-5p PDGFRA -0.26834261955727 0.00453760540520233 -0.214227835759073 0.123422750860196 mirMAP -0.403170180391648 4.54797630182334e-11 NA NA NA hsa-miR-29b-3p PDGFRA -0.178522712044497 0.0856106432165465 -0.214227835759073 0.123422750860196 MirTarget -0.479354783825126 2.78031130409556e-15 NA NA NA hsa-miR-29c-3p PDGFRA -0.372180390387259 0.00202634431973208 -0.214227835759073 0.123422750860196 MirTarget -0.325784473603734 3.2768258385201e-10 NA NA NA hsa-miR-320c PDGFRA 0.343259124189966 0.000248338401291051 -0.214227835759073 0.123422750860196 miRanda -0.25132609270931 6.63976805483441e-05 NA NA NA hsa-miR-330-5p PDGFRA -0.262932095995605 0.0465226565966658 -0.214227835759073 0.123422750860196 miRanda -0.122999029913756 0.00489006932093651 NA NA NA hsa-miR-335-3p PDGFRA 0.34540584335595 0.00043848660222294 -0.214227835759073 0.123422750860196 mirMAP -0.296573735389458 8.96271541553145e-07 NA NA NA hsa-miR-339-5p PDGFRA 0.273253751377152 0.0185300170790638 -0.214227835759073 0.123422750860196 miRanda -0.331337860958994 1.16346191528288e-11 NA NA NA hsa-miR-34a-5p PDGFRA 0.143472919835524 0.211283228567057 -0.214227835759073 0.123422750860196 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.507657602010947 6.25800263585505e-25 22750848 Mechanistically in addition to its direct regulation of platelet-derived growth factor receptor-alpha PDGFRA promoter enrichment analysis of context likelihood of relatedness-inferred mRNA nodes established miR-34a as a novel regulator of a SMAD4 transcriptional network glioblastoma hsa-miR-34c-5p PDGFRA 0.181931113688093 0.257266965617363 -0.214227835759073 0.123422750860196 MirTarget;PITA;miRanda;miRNATAP -0.238616457279072 8.42235088990927e-12 NA NA NA hsa-miR-362-3p PDGFRA 0.032375035235532 0.736465332563774 -0.214227835759073 0.123422750860196 miRanda -0.25592920204664 1.90006109239814e-05 NA NA NA hsa-miR-455-5p PDGFRA 0.332224239762 0.0106491358056238 -0.214227835759073 0.123422750860196 MirTarget;PITA;miRanda -0.206982425909943 2.64647669398927e-06 NA NA NA hsa-miR-488-3p PDGFRA -0.257955761012568 0.00280795162806256 -0.214227835759073 0.123422750860196 mirMAP -0.245006697838914 0.000325177624269067 NA NA NA hsa-miR-576-5p PDGFRA 0.106482276040047 0.194379152564166 -0.214227835759073 0.123422750860196 mirMAP -0.275734374551186 0.000105383234164016 NA NA NA hsa-miR-590-3p PDGFRA 0.312951515438396 0.000480455423843996 -0.214227835759073 0.123422750860196 miRanda;mirMAP -0.360558237867372 1.42959874478908e-08 NA NA NA hsa-miR-590-5p PDGFRA 0.16946027035949 0.0386004382081678 -0.214227835759073 0.123422750860196 miRanda -0.339783423553957 1.57951301556451e-06 NA NA NA hsa-miR-664a-3p PDGFRA 0.0413918462467198 0.611334357897022 -0.214227835759073 0.123422750860196 mirMAP -0.68336198484181 1.47148431801351e-22 NA NA NA hsa-miR-7-1-3p PDGFRA -0.0894475316162393 0.37322359785108 -0.214227835759073 0.123422750860196 mirMAP -0.241177783617488 2.86345919927891e-05 NA NA NA hsa-miR-9-5p PDGFRB -0.125377971246422 0.269896477802284 0.193259964403652 0.00272288258408316 MirTarget;miRNATAP -0.367539214293885 4.33000477969503e-12 NA NA NA hsa-miR-320a PDPK1 0.092135599636725 0.243028758989929 -0.124922548123071 0.0272075929298163 miRanda -0.122231777017362 8.84868459069661e-05 NA NA NA hsa-miR-374a-3p PDPK1 0.176226170437625 0.0343958848425446 -0.124922548123071 0.0272075929298163 mirMAP -0.151634704051745 2.27533108343501e-07 NA NA NA hsa-miR-423-5p PDPK1 0.0965587900813789 0.148039180704338 -0.124922548123071 0.0272075929298163 miRNAWalker2_validate -0.124647638553822 0.000175919526142779 NA NA NA hsa-miR-93-5p PDPK1 0.139766237971122 0.101057320076629 -0.124922548123071 0.0272075929298163 mirMAP -0.199867731891363 1.51820751789459e-08 NA NA NA hsa-miR-124-3p PGF -0.506026514549291 0.0078056370358648 -0.341184446598072 0.02198133567065 MirTarget;miRNATAP -0.12378981779743 0.000279266195514154 NA NA NA hsa-miR-142-3p PGF 0.558908907203977 1.37828834702416e-05 -0.341184446598072 0.02198133567065 miRanda -0.209524698619111 4.8489187207237e-05 NA NA NA hsa-miR-146b-5p PGF 0.441078059667297 0.000237619929889385 -0.341184446598072 0.02198133567065 miRanda -0.219423284623696 9.73569588345958e-05 NA NA NA hsa-miR-149-5p PGF -0.0535821592029952 0.657713892012548 -0.341184446598072 0.02198133567065 miRNATAP -0.335460001684344 4.97608776107746e-10 NA NA NA hsa-miR-184 PGF -0.842534984406592 6.08844932016991e-08 -0.341184446598072 0.02198133567065 miRanda -0.136337291100717 0.000950149149887701 NA NA NA hsa-miR-199b-5p PGF 0.436298593972862 0.00200225454383871 -0.341184446598072 0.02198133567065 miRanda -0.150774194706357 0.000927470972217645 NA NA NA hsa-miR-335-5p PGF 0.0938511632756978 0.336889056387694 -0.341184446598072 0.02198133567065 miRNATAP -0.314497501310152 2.85681687322176e-06 NA NA NA hsa-miR-34a-5p PGF 0.143472919835524 0.211283228567057 -0.341184446598072 0.02198133567065 miRNATAP -0.279835357608525 1.1066200175762e-06 NA NA NA hsa-miR-766-3p PGF -0.147747825439875 0.266658981351507 -0.341184446598072 0.02198133567065 MirTarget -0.244529156614663 3.20505159007913e-07 NA NA NA hsa-miR-126-5p PHLPP1 0.177332403948664 0.0711675116243123 -0.214881473231678 0.000673135698085585 MirTarget;miRNATAP -0.112365126560071 1.51325271659891e-07 NA NA NA hsa-miR-1307-3p PHLPP1 -0.065481322115085 0.445858153142067 -0.214881473231678 0.000673135698085585 miRNAWalker2_validate -0.220084409863922 3.30210306831632e-15 NA NA NA hsa-miR-141-3p PHLPP1 0.41120561991992 0.000393344390969055 -0.214881473231678 0.000673135698085585 MirTarget;TargetScan;miRNATAP -0.121740245122954 2.6503914414182e-12 24945731 MicroRNA 141 promotes the proliferation of non small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2; Together the results of this study suggest that miR-141 and its targets PHLPP1 and PHLPP2 play critical roles in NSCLC tumorigenesis and provide potential therapeutic targets for NSCLC treatment tumorigenesis lung squamous cell cancer hsa-miR-16-2-3p PHLPP1 0.468816222075619 7.04377763166292e-05 -0.214881473231678 0.000673135698085585 MirTarget -0.147086656639281 3.26663101026531e-19 NA NA NA hsa-miR-29b-1-5p PHLPP1 0.191335428755774 0.00846436454790702 -0.214881473231678 0.000673135698085585 MirTarget -0.153758929266115 2.36985247627348e-08 NA NA NA hsa-miR-452-5p PHLPP1 0.599327068454211 1.02031931068531e-05 -0.214881473231678 0.000673135698085585 MirTarget;miRNATAP -0.123432754790907 6.27977856459226e-18 NA NA NA hsa-miR-484 PHLPP1 -0.0190828819137732 0.802298900989652 -0.214881473231678 0.000673135698085585 miRNAWalker2_validate -0.167490631166816 2.81298691635043e-09 NA NA NA hsa-miR-589-5p PHLPP1 -0.0382528919634639 0.5844074434222 -0.214881473231678 0.000673135698085585 MirTarget -0.198255442021565 2.61395855331512e-09 NA NA NA hsa-miR-590-3p PHLPP1 0.312951515438396 0.000480455423843996 -0.214881473231678 0.000673135698085585 miRanda -0.136310586727697 9.19568090917848e-10 NA NA NA hsa-miR-106b-5p PHLPP2 0.167096183547615 0.0497544396383451 -0.295383088255838 3.01170349547581e-07 miRNATAP -0.137767114553835 3.48084982570878e-06 NA NA NA hsa-miR-135a-5p PHLPP2 -0.122734612943241 0.196985964641886 -0.295383088255838 3.01170349547581e-07 miRNATAP -0.112364088044943 3.66938074826813e-06 25888950 Mir 135a enhances cellular proliferation through post transcriptionally regulating PHLPP2 and FOXO1 in human bladder cancer; Furthermore for the first time we demonstrated PHLPP2 and FOXO1 are direct targets of miR-135a and transcriptionally down-regulated by miR-135a; Our study demonstrates that miR-135a promotes cell proliferation in bladder cancer by targeting PHLPP2 and FOXO1 and is performed as an onco-miR bladder cancer hsa-miR-141-3p PHLPP2 0.41120561991992 0.000393344390969055 -0.295383088255838 3.01170349547581e-07 MirTarget;TargetScan;miRNATAP -0.130485900032813 1.40845889986558e-11 24945731 MicroRNA 141 promotes the proliferation of non small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2; Together the results of this study suggest that miR-141 and its targets PHLPP1 and PHLPP2 play critical roles in NSCLC tumorigenesis and provide potential therapeutic targets for NSCLC treatment tumorigenesis lung squamous cell cancer hsa-miR-142-5p PHLPP2 0.434884875454107 0.000575570345702788 -0.295383088255838 3.01170349547581e-07 mirMAP -0.105099037534289 1.56882011860842e-09 NA NA NA hsa-miR-15a-5p PHLPP2 0.142658985373611 0.0536219185009004 -0.295383088255838 3.01170349547581e-07 MirTarget;miRNATAP -0.172860109993957 2.17062889128289e-06 NA NA NA hsa-miR-15b-5p PHLPP2 0.592863156692837 1.41971517354055e-10 -0.295383088255838 3.01170349547581e-07 MirTarget;miRNATAP -0.171979606291794 6.25996092652675e-12 NA NA NA hsa-miR-17-5p PHLPP2 0.0975751369167082 0.20621110214601 -0.295383088255838 3.01170349547581e-07 TargetScan;miRNATAP -0.10602179055354 0.00331769967294842 NA NA NA hsa-miR-186-5p PHLPP2 0.0606381894130505 0.514217717592464 -0.295383088255838 3.01170349547581e-07 mirMAP -0.173101896001868 3.83722803101622e-11 NA NA NA hsa-miR-18a-5p PHLPP2 0.175320895022849 0.0732549187136286 -0.295383088255838 3.01170349547581e-07 miRNATAP -0.101514225320767 7.9491091930934e-06 NA NA NA hsa-miR-19a-3p PHLPP2 0.0466707843731911 0.576818938277755 -0.295383088255838 3.01170349547581e-07 mirMAP -0.115320091040705 3.85539100696462e-05 NA NA NA hsa-miR-19b-3p PHLPP2 -0.0138427450847924 0.856506310673067 -0.295383088255838 3.01170349547581e-07 mirMAP -0.128123779484888 0.000126764733322101 NA NA NA hsa-miR-21-3p PHLPP2 1.3484283943822 2.0036022227401e-17 -0.295383088255838 3.01170349547581e-07 mirMAP -0.118692879674515 1.25900689857913e-18 NA NA NA hsa-miR-25-3p PHLPP2 0.316649850160985 0.000777982468387578 -0.295383088255838 3.01170349547581e-07 MirTarget;miRNATAP -0.17711676886839 8.76664781969928e-09 NA NA NA hsa-miR-26a-5p PHLPP2 0.033823776747294 0.726610414342369 -0.295383088255838 3.01170349547581e-07 mirMAP -0.123245637238055 1.07890853842225e-05 NA NA NA hsa-miR-26b-5p PHLPP2 -0.0984536538762768 0.231803769574623 -0.295383088255838 3.01170349547581e-07 miRNAWalker2_validate -0.207284734483285 1.13442932636638e-10 NA NA NA hsa-miR-27a-3p PHLPP2 0.303760032895792 0.00457375391189806 -0.295383088255838 3.01170349547581e-07 MirTarget;miRNATAP -0.183437147341682 7.62315401541749e-17 NA NA NA hsa-miR-28-5p PHLPP2 0.104003572105781 0.153594705579534 -0.295383088255838 3.01170349547581e-07 miRanda -0.186274959068024 1.40403347131318e-08 NA NA NA hsa-miR-32-5p PHLPP2 0.130313603388223 0.177843055266148 -0.295383088255838 3.01170349547581e-07 miRNAWalker2_validate;MirTarget;miRNATAP -0.120064193609302 3.02429301195821e-07 26276160 MiR 32 contributed to cell proliferation of human breast cancer cells by suppressing of PHLPP2 expression; Mechanically data from luciferase reporter assays revealed that miR-32 directly targeted to the 3'-untranslated region 3'-UTR of PHLPP2 breast cancer hsa-miR-421 PHLPP2 -0.171573235029879 0.0518112933651029 -0.295383088255838 3.01170349547581e-07 miRNAWalker2_validate;miRanda -0.100515218950079 7.85012772700749e-05 NA NA NA hsa-miR-590-3p PHLPP2 0.312951515438396 0.000480455423843996 -0.295383088255838 3.01170349547581e-07 miRanda -0.190455195750638 5.84011425904108e-15 NA NA NA hsa-miR-590-5p PHLPP2 0.16946027035949 0.0386004382081678 -0.295383088255838 3.01170349547581e-07 miRanda -0.161293394565174 3.78204125814921e-09 NA NA NA hsa-miR-92b-3p PHLPP2 -0.213968504161963 0.0523921855715716 -0.295383088255838 3.01170349547581e-07 MirTarget;miRNATAP -0.108758685916183 3.84222918993287e-06 NA NA NA hsa-miR-93-5p PHLPP2 0.139766237971122 0.101057320076629 -0.295383088255838 3.01170349547581e-07 miRNAWalker2_validate;miRNATAP -0.260514849854687 7.29452146663393e-11 NA NA NA hsa-miR-139-5p PIK3AP1 -0.647754756897633 2.15378307237163e-06 0.255915820809661 0.00631029016387649 miRanda -0.240906416252318 2.66551359466428e-16 NA NA NA hsa-miR-582-5p PIK3AP1 -0.215764335500665 0.0760892640434509 0.255915820809661 0.00631029016387649 MirTarget -0.251253116773166 3.89425011149779e-15 NA NA NA hsa-miR-146b-5p PIK3CA 0.441078059667297 0.000237619929889385 0.037489443318349 0.579330715365297 mirMAP -0.131612247931155 2.37773318343185e-08 NA NA NA hsa-miR-339-5p PIK3CA 0.273253751377152 0.0185300170790638 0.037489443318349 0.579330715365297 miRanda -0.104034425659594 8.90794530378182e-06 NA NA NA hsa-miR-361-5p PIK3CA 0.0107519600643302 0.882230614095565 0.037489443318349 0.579330715365297 mirMAP -0.180837597518142 0.000106487510577531 NA NA NA hsa-miR-501-5p PIK3CA 0.0878951859817452 0.309663377222443 0.037489443318349 0.579330715365297 mirMAP -0.121849868853655 0.000118457183740553 NA NA NA hsa-miR-130b-3p PIK3CB 0.503911467685709 9.46590588950823e-08 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.103286697323809 1.47078743054601e-08 NA NA NA hsa-miR-19a-3p PIK3CB 0.0466707843731911 0.576818938277755 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.106776286308973 5.5364329421429e-07 NA NA NA hsa-miR-19b-3p PIK3CB -0.0138427450847924 0.856506310673067 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.144620281397534 1.13083007810642e-08 NA NA NA hsa-miR-25-3p PIK3CB 0.316649850160985 0.000777982468387578 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.107740273525281 5.28619562870356e-06 NA NA NA hsa-miR-126-3p PIK3CG 0.283844173647452 0.0045313860754322 0.426342297358177 0.000910429107358105 miRTarBase -0.253640716789511 9.06991114742445e-05 NA NA NA hsa-miR-137 PIK3CG -0.924849266740909 1.546832202049e-06 0.426342297358177 0.000910429107358105 miRanda -0.150716294097788 1.51412490486832e-07 NA NA NA hsa-miR-139-5p PIK3CG -0.647754756897633 2.15378307237163e-06 0.426342297358177 0.000910429107358105 miRanda -0.231442980598987 3.92120693342636e-08 NA NA NA hsa-miR-103a-3p PIK3R1 -0.147443193527362 0.128400914654149 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.281302889120328 4.63842851180526e-09 NA NA NA hsa-miR-106a-5p PIK3R1 0.110074064773897 0.13045815990621 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.141577511066847 0.000568718663709082 NA NA NA hsa-miR-106b-5p PIK3R1 0.167096183547615 0.0497544396383451 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.114620357579885 0.00229922204074265 NA NA NA hsa-miR-1301-3p PIK3R1 -0.105695714210758 0.152249184194132 -0.259275224308753 0.00106935451973482 MirTarget -0.230097168026832 5.13675721710063e-09 NA NA NA hsa-miR-150-5p PIK3R1 0.0645271721519611 0.513295658828718 -0.259275224308753 0.00106935451973482 MirTarget -0.105207403299517 0.000393418533820088 NA NA NA hsa-miR-15a-5p PIK3R1 0.142658985373611 0.0536219185009004 -0.259275224308753 0.00106935451973482 MirTarget -0.230258286136212 5.21596791619245e-07 NA NA NA hsa-miR-15b-5p PIK3R1 0.592863156692837 1.41971517354055e-10 -0.259275224308753 0.00106935451973482 MirTarget -0.235643490684548 5.7801231863435e-14 NA NA NA hsa-miR-16-2-3p PIK3R1 0.468816222075619 7.04377763166292e-05 -0.259275224308753 0.00106935451973482 MirTarget -0.139286207343494 2.58317956279833e-09 NA NA NA hsa-miR-16-5p PIK3R1 0.246024668671282 0.00346042804503716 -0.259275224308753 0.00106935451973482 MirTarget -0.121551450392141 0.00217223263389569 NA NA NA hsa-miR-185-5p PIK3R1 -0.0767432095218297 0.404113540276816 -0.259275224308753 0.00106935451973482 miRNATAP -0.168972197052794 1.52142067802957e-07 NA NA NA hsa-miR-186-5p PIK3R1 0.0606381894130505 0.514217717592464 -0.259275224308753 0.00106935451973482 mirMAP -0.273384834286173 4.58184035796298e-17 NA NA NA hsa-miR-199a-3p PIK3R1 0.41226331871076 0.000497136154474528 -0.259275224308753 0.00106935451973482 miRNATAP -0.150441413618647 2.58272678316472e-10 NA NA NA hsa-miR-199a-5p PIK3R1 0.174889610847968 0.0900027418912454 -0.259275224308753 0.00106935451973482 miRanda -0.169409376623758 6.00323276983558e-10 NA NA NA hsa-miR-199b-3p PIK3R1 0.411848361704393 0.000517815011892379 -0.259275224308753 0.00106935451973482 miRNATAP -0.149610269102807 2.9670988718931e-10 NA NA NA hsa-miR-199b-5p PIK3R1 0.436298593972862 0.00200225454383871 -0.259275224308753 0.00106935451973482 miRanda -0.158988773915679 1.96615064731078e-16 NA NA NA hsa-miR-200b-3p PIK3R1 0.245768526504511 0.0580744465744737 -0.259275224308753 0.00106935451973482 mirMAP -0.203398688722103 1.6080278648636e-21 NA NA NA hsa-miR-200c-3p PIK3R1 0.413752622631695 8.6936333715621e-06 -0.259275224308753 0.00106935451973482 mirMAP -0.313372851003899 1.11853298374691e-27 NA NA NA hsa-miR-21-5p PIK3R1 1.0504925175311 2.58081295984106e-11 -0.259275224308753 0.00106935451973482 miRNAWalker2_validate;MirTarget;miRNATAP -0.192193122796698 2.35557430088274e-23 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-22-5p PIK3R1 0.0437233965539527 0.639996108995995 -0.259275224308753 0.00106935451973482 mirMAP -0.26421716056902 5.35024336821731e-19 NA NA NA hsa-miR-221-3p PIK3R1 -0.0109507285071775 0.936440693394519 -0.259275224308753 0.00106935451973482 MirTarget -0.125242954911868 1.02528886125279e-09 NA NA NA hsa-miR-222-3p PIK3R1 0.107117065575197 0.423818119725697 -0.259275224308753 0.00106935451973482 MirTarget -0.132930686168698 1.29543187744781e-10 NA NA NA hsa-miR-28-5p PIK3R1 0.104003572105781 0.153594705579534 -0.259275224308753 0.00106935451973482 miRanda -0.263804174247158 1.47407900801522e-10 NA NA NA hsa-miR-29b-3p PIK3R1 -0.178522712044497 0.0856106432165465 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.145678229090259 1.47483484512088e-06 NA NA NA hsa-miR-320a PIK3R1 0.092135599636725 0.243028758989929 -0.259275224308753 0.00106935451973482 miRNATAP -0.135112165926423 0.00256497204146449 NA NA NA hsa-miR-320c PIK3R1 0.343259124189966 0.000248338401291051 -0.259275224308753 0.00106935451973482 miRNATAP -0.100797336995439 0.0011155444118643 NA NA NA hsa-miR-361-5p PIK3R1 0.0107519600643302 0.882230614095565 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.28102979614435 4.07903189307061e-09 NA NA NA hsa-miR-374a-3p PIK3R1 0.176226170437625 0.0343958848425446 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.321250741910251 7.52372972851822e-15 NA NA NA hsa-miR-424-5p PIK3R1 0.25140374049676 0.00709934939774983 -0.259275224308753 0.00106935451973482 MirTarget -0.179589895147171 9.52256678975884e-09 NA NA NA hsa-miR-455-3p PIK3R1 0.512701816402104 0.000459755983280155 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRNATAP -0.161697245880861 9.23450362337655e-18 NA NA NA hsa-miR-532-3p PIK3R1 0.150624483952741 0.0599635132177191 -0.259275224308753 0.00106935451973482 MirTarget -0.152797062588971 2.75979429090484e-05 NA NA NA hsa-miR-542-3p PIK3R1 0.0835995677900829 0.403838744494071 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRanda -0.126538995109648 4.83333922747737e-05 NA NA NA hsa-miR-548b-3p PIK3R1 0.340474544210108 0.00224491592019745 -0.259275224308753 0.00106935451973482 mirMAP -0.215757322792441 3.83990685247808e-18 NA NA NA hsa-miR-589-3p PIK3R1 0.00739371736361305 0.927768051649329 -0.259275224308753 0.00106935451973482 mirMAP -0.128475712773886 0.000234342022369328 NA NA NA hsa-miR-590-3p PIK3R1 0.312951515438396 0.000480455423843996 -0.259275224308753 0.00106935451973482 miRanda;mirMAP -0.281948259643294 1.55243456524174e-20 NA NA NA hsa-miR-590-5p PIK3R1 0.16946027035949 0.0386004382081678 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRanda;miRNATAP -0.237039982006307 4.44595475697952e-12 NA NA NA hsa-miR-93-5p PIK3R1 0.139766237971122 0.101057320076629 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.261542441732551 2.52352152477154e-07 NA NA NA hsa-miR-125a-3p PIK3R2 0.183834985432376 0.106230647107917 0.0588428389717066 0.330993461191286 miRanda -0.129596879826368 7.29774749644301e-14 NA NA NA hsa-miR-135a-5p PIK3R2 -0.122734612943241 0.196985964641886 0.0588428389717066 0.330993461191286 MirTarget;miRNATAP -0.131986778854444 6.81341317924018e-10 NA NA NA hsa-miR-28-5p PIK3R2 0.104003572105781 0.153594705579534 0.0588428389717066 0.330993461191286 miRanda -0.220439409614541 1.97006000122873e-14 NA NA NA hsa-miR-30a-5p PIK3R2 -0.062942936611238 0.513851954700252 0.0588428389717066 0.330993461191286 miRNATAP -0.422270293356803 9.55851299393926e-41 NA NA NA hsa-miR-30b-5p PIK3R2 -0.0858101016322106 0.263243418521854 0.0588428389717066 0.330993461191286 miRNATAP -0.124149959343624 7.09161751121272e-05 NA NA NA hsa-miR-30c-5p PIK3R2 -0.0469114469943097 0.565934557464627 0.0588428389717066 0.330993461191286 miRNAWalker2_validate;miRNATAP -0.240340425585456 4.99963373312426e-15 NA NA NA hsa-miR-30d-5p PIK3R2 -0.0607010868720046 0.471410043548794 0.0588428389717066 0.330993461191286 miRNATAP -0.157898559984916 9.76384983421278e-06 NA NA NA hsa-miR-30e-5p PIK3R2 0.0764521828640934 0.41904991629622 0.0588428389717066 0.330993461191286 miRNATAP -0.28288573453624 2.98060149038092e-23 NA NA NA hsa-miR-331-3p PIK3R2 0.0126984726684189 0.905175053956974 0.0588428389717066 0.330993461191286 MirTarget -0.109375813751675 5.11486213404741e-09 NA NA NA hsa-miR-339-5p PIK3R2 0.273253751377152 0.0185300170790638 0.0588428389717066 0.330993461191286 miRanda -0.141580109769359 1.93632849649246e-17 NA NA NA hsa-miR-34a-5p PIK3R2 0.143472919835524 0.211283228567057 0.0588428389717066 0.330993461191286 MirTarget -0.127777793508925 2.24958566486742e-13 NA NA NA hsa-miR-9-3p PIK3R3 -0.184945845984695 0.0290051908009009 0.266207970572364 5.77735138484935e-05 mirMAP -0.147414732691119 0.000303043365501486 NA NA NA hsa-miR-9-5p PIK3R3 -0.125377971246422 0.269896477802284 0.266207970572364 5.77735138484935e-05 miRNATAP -0.234645741849474 2.452186835562e-05 NA NA NA hsa-miR-425-3p PKN1 -0.173302979693293 0.0801548384038174 0.0531964429866507 0.393680266532473 miRNAWalker2_validate -0.159300210443682 3.23792325368591e-16 NA NA NA hsa-miR-105-5p PKN2 -0.567459149851677 8.20858944634795e-06 0.108809113666802 0.0675308137800777 MirTarget;miRNATAP -0.121166891983124 4.75434623763303e-12 NA NA NA hsa-miR-137 PKN2 -0.924849266740909 1.546832202049e-06 0.108809113666802 0.0675308137800777 MirTarget;PITA;miRanda;miRNATAP -0.108881296674983 3.56466735000315e-23 NA NA NA hsa-miR-153-3p PKN2 -0.173449510253619 0.0654368045743418 0.108809113666802 0.0675308137800777 MirTarget -0.154487614500443 2.33900234976446e-10 NA NA NA hsa-miR-346 PKN2 -0.577786425963268 2.10883270131593e-09 0.108809113666802 0.0675308137800777 miRNATAP -0.130432671009394 9.43722943434701e-09 NA NA NA hsa-miR-582-3p PKN2 -0.11807589183011 0.304077332003677 0.108809113666802 0.0675308137800777 miRNATAP -0.176561750199241 4.56497194411665e-20 NA NA NA hsa-miR-324-3p PKN3 -0.0103367134443655 0.904494071318044 0.547429972777229 6.48768157239915e-16 miRNAWalker2_validate -0.123292713912629 0.000670066721413882 NA NA NA hsa-miR-30a-5p PPP2R2A -0.062942936611238 0.513851954700252 0.0278684403282758 0.506122646610165 miRNAWalker2_validate -0.122723302846802 5.02059408015066e-09 NA NA NA hsa-miR-320a PPP2R2C 0.092135599636725 0.243028758989929 -0.807106221858617 5.62171269755685e-07 MirTarget;PITA;miRanda;miRNATAP -0.559687502304299 5.73079947467746e-07 NA NA NA hsa-miR-320b PPP2R2C 0.283477746304177 0.0005953763020907 -0.807106221858617 5.62171269755685e-07 MirTarget;PITA;miRanda;miRNATAP -0.419378394628078 8.73686592064403e-07 NA NA NA hsa-miR-320c PPP2R2C 0.343259124189966 0.000248338401291051 -0.807106221858617 5.62171269755685e-07 MirTarget;PITA;miRanda;miRNATAP -0.45106636838668 4.62037237222019e-09 NA NA NA hsa-miR-590-3p PPP2R2C 0.312951515438396 0.000480455423843996 -0.807106221858617 5.62171269755685e-07 miRanda -0.221384246989803 0.00522969466907128 NA NA NA hsa-miR-27a-3p PPP2R2D 0.303760032895792 0.00457375391189806 -0.162763779743182 0.000323597056727485 mirMAP -0.149138610183681 2.5572398627466e-21 NA NA NA hsa-miR-590-3p PPP2R2D 0.312951515438396 0.000480455423843996 -0.162763779743182 0.000323597056727485 miRanda;mirMAP -0.106104652230053 2.62037052357913e-09 NA NA NA hsa-miR-320a PPP2R3A 0.092135599636725 0.243028758989929 -0.145118969641774 0.0125235991574421 miRanda -0.191409790870392 3.05048194336898e-07 NA NA NA hsa-miR-181c-5p PPP2R3B -0.285631740307867 0.00281600994521758 0.0697014494830128 0.378720957258046 MirTarget -0.182929416372287 1.63058310394595e-06 NA NA NA hsa-miR-192-5p PPP2R3B -0.0426540615456616 0.621616927627554 0.0697014494830128 0.378720957258046 miRNAWalker2_validate -0.191078881971687 1.58243170619539e-05 NA NA NA hsa-miR-107 PPP2R5A -0.121070851008387 0.198381281894242 -0.560968743573282 1.28924141102638e-12 miRanda -0.137312868546319 0.000101059157362503 NA NA NA hsa-miR-299-5p PPP2R5A -0.0814737109317336 0.57620244226785 -0.560968743573282 1.28924141102638e-12 PITA;miRNATAP -0.102718337044819 2.18651702505648e-06 NA NA NA hsa-miR-335-5p PPP2R5A 0.0938511632756978 0.336889056387694 -0.560968743573282 1.28924141102638e-12 miRNAWalker2_validate -0.173366581478925 9.81646000324009e-08 NA NA NA hsa-miR-34a-5p PPP2R5A 0.143472919835524 0.211283228567057 -0.560968743573282 1.28924141102638e-12 MirTarget;miRNATAP -0.183627035968764 2.98949511678076e-11 NA NA NA hsa-miR-34c-5p PPP2R5A 0.181931113688093 0.257266965617363 -0.560968743573282 1.28924141102638e-12 MirTarget;PITA;miRanda;miRNATAP -0.122979122974169 1.16976245363982e-10 NA NA NA hsa-miR-582-5p PPP2R5A -0.215764335500665 0.0760892640434509 -0.560968743573282 1.28924141102638e-12 PITA;miRNATAP -0.110269895061871 1.57160284780262e-05 NA NA NA hsa-miR-590-3p PPP2R5A 0.312951515438396 0.000480455423843996 -0.560968743573282 1.28924141102638e-12 miRNAWalker2_validate -0.109469467724205 0.00178010792298095 NA NA NA hsa-miR-146b-5p PPP2R5C 0.441078059667297 0.000237619929889385 -0.254272948072342 1.69001734328025e-06 miRanda -0.104636941944054 6.44013765390368e-15 NA NA NA hsa-miR-15a-5p PPP2R5C 0.142658985373611 0.0536219185009004 -0.254272948072342 1.69001734328025e-06 miRNATAP -0.200156920569006 1.85098904717096e-15 NA NA NA hsa-miR-15b-5p PPP2R5C 0.592863156692837 1.41971517354055e-10 -0.254272948072342 1.69001734328025e-06 miRNATAP -0.205121300896824 2.364669413921e-34 NA NA NA hsa-miR-16-5p PPP2R5C 0.246024668671282 0.00346042804503716 -0.254272948072342 1.69001734328025e-06 miRNAWalker2_validate;miRNATAP -0.16388416042408 3.74945792569191e-14 NA NA NA hsa-miR-28-3p PPP2R5C 0.139444423971018 0.0811187936164314 -0.254272948072342 1.69001734328025e-06 PITA -0.115674445248112 5.47154420556708e-06 NA NA NA hsa-let-7a-3p PPP2R5E 0.0530563276543763 0.370749834463566 -0.00642086237028749 0.899053449389186 mirMAP -0.113525985389828 0.00166602460317483 NA NA NA hsa-miR-30a-5p PPP2R5E -0.062942936611238 0.513851954700252 -0.00642086237028749 0.899053449389186 mirMAP -0.194674812505274 1.76897834837535e-09 NA NA NA hsa-miR-30b-5p PPP2R5E -0.0858101016322106 0.263243418521854 -0.00642086237028749 0.899053449389186 mirMAP -0.182048118174176 8.27885941296834e-10 NA NA NA hsa-miR-30c-5p PPP2R5E -0.0469114469943097 0.565934557464627 -0.00642086237028749 0.899053449389186 mirMAP -0.213723164961204 4.89620491763908e-13 NA NA NA hsa-miR-361-5p PPP2R5E 0.0107519600643302 0.882230614095565 -0.00642086237028749 0.899053449389186 miRanda -0.101133170947133 0.00199076639213648 NA NA NA hsa-miR-365a-3p PPP2R5E -0.184222766207919 0.0292663235724171 -0.00642086237028749 0.899053449389186 miRNAWalker2_validate -0.10652573303036 3.84386723765993e-06 NA NA NA hsa-miR-374a-3p PPP2R5E 0.176226170437625 0.0343958848425446 -0.00642086237028749 0.899053449389186 MirTarget -0.120569813550011 2.35701836214203e-05 NA NA NA hsa-miR-500a-3p PPP2R5E 0.0479457078972052 0.49420307623914 -0.00642086237028749 0.899053449389186 MirTarget -0.1575021564293 7.55930237003387e-07 NA NA NA hsa-miR-502-3p PPP2R5E 0.0276791089364088 0.697605011368848 -0.00642086237028749 0.899053449389186 MirTarget;PITA;miRNATAP -0.102033264015188 0.000178779831614998 NA NA NA hsa-miR-9-3p PPP2R5E -0.184945845984695 0.0290051908009009 -0.00642086237028749 0.899053449389186 mirMAP -0.103118267073452 0.000768700677345424 NA NA NA hsa-miR-146a-5p PRKAA2 0.148942451196282 0.114229072828724 -0.153760632830788 0.0160802531184971 MirTarget -0.136216138680821 1.5698093569435e-06 NA NA NA hsa-miR-195-3p PRKAA2 0.100105455903079 0.133362389112338 -0.153760632830788 0.0160802531184971 mirMAP -0.111515203087672 0.00669731329074443 NA NA NA hsa-miR-146b-5p PRKCA 0.441078059667297 0.000237619929889385 -0.289954367502515 3.07027667899011e-05 mirMAP -0.14319708794989 9.06517098676715e-13 NA NA NA hsa-miR-186-5p PRKCA 0.0606381894130505 0.514217717592464 -0.289954367502515 3.07027667899011e-05 miRNATAP -0.150359410939945 4.84077626913968e-08 NA NA NA hsa-miR-1976 PRKCA 0.127074435034705 0.277235601775457 -0.289954367502515 3.07027667899011e-05 miRNATAP -0.108813705938178 3.46283535790712e-08 NA NA NA hsa-miR-200c-3p PRKCA 0.413752622631695 8.6936333715621e-06 -0.289954367502515 3.07027667899011e-05 miRNATAP -0.178242109766722 4.41123343449352e-13 NA NA NA hsa-miR-22-5p PRKCA 0.0437233965539527 0.639996108995995 -0.289954367502515 3.07027667899011e-05 mirMAP -0.109722618118012 1.49794234596401e-05 NA NA NA hsa-miR-24-2-5p PRKCA 0.285455874963858 0.00610163594056254 -0.289954367502515 3.07027667899011e-05 miRNAWalker2_validate -0.126181943667759 1.61489847928406e-08 22911661 Preferential star strand biogenesis of pre miR 24 2 targets PKC alpha and suppresses cell survival in MCF 7 breast cancer cells poor survival breast cancer hsa-miR-27b-5p PRKCA 0.224282840351546 0.00630787296051719 -0.289954367502515 3.07027667899011e-05 mirMAP -0.177695243384062 3.0717917979023e-10 NA NA NA hsa-miR-339-5p PRKCA 0.273253751377152 0.0185300170790638 -0.289954367502515 3.07027667899011e-05 mirMAP -0.144247762214447 3.06350803308541e-13 NA NA NA hsa-miR-374a-3p PRKCA 0.176226170437625 0.0343958848425446 -0.289954367502515 3.07027667899011e-05 mirMAP -0.183155883331884 1.46022453353189e-07 NA NA NA hsa-miR-452-5p PRKCA 0.599327068454211 1.02031931068531e-05 -0.289954367502515 3.07027667899011e-05 mirMAP -0.107144316873697 1.95734554580544e-10 NA NA NA hsa-miR-500a-5p PRKCA 0.0634452729978863 0.396072382601677 -0.289954367502515 3.07027667899011e-05 miRNATAP -0.184254955997398 3.38268451218896e-09 NA NA NA hsa-miR-501-5p PRKCA 0.0878951859817452 0.309663377222443 -0.289954367502515 3.07027667899011e-05 miRNATAP -0.110873198819 4.40374990873549e-05 NA NA NA hsa-miR-505-3p PRKCA 0.115486008167472 0.109833720698312 -0.289954367502515 3.07027667899011e-05 miRNAWalker2_validate -0.183149886354061 7.20630466764891e-08 NA NA NA hsa-miR-505-5p PRKCA 0.0973008785018434 0.183209581426791 -0.289954367502515 3.07027667899011e-05 mirMAP -0.102672793703942 0.00160299774148607 NA NA NA hsa-miR-548b-3p PRKCA 0.340474544210108 0.00224491592019745 -0.289954367502515 3.07027667899011e-05 PITA;miRNATAP -0.107410539931332 3.63814329290144e-07 NA NA NA hsa-miR-550a-3p PRKCA 0.311518294008363 0.0015285640451888 -0.289954367502515 3.07027667899011e-05 miRNATAP -0.135787662750574 7.76840887594873e-09 NA NA NA hsa-miR-590-3p PRKCA 0.312951515438396 0.000480455423843996 -0.289954367502515 3.07027667899011e-05 PITA;miRanda;mirMAP;miRNATAP -0.152536946782924 3.436144371863e-09 NA NA NA hsa-miR-93-3p PRKCA 0.0778767956494004 0.272224943247293 -0.289954367502515 3.07027667899011e-05 mirMAP -0.146551656012825 2.11995627214635e-05 NA NA NA hsa-miR-454-3p PRLR 0.177318549827649 0.0120865170334418 -0.090634989600836 0.414123305437059 mirMAP -0.188829502619545 0.00819269251358993 NA NA NA hsa-miR-9-3p PRLR -0.184945845984695 0.0290051908009009 -0.090634989600836 0.414123305437059 mirMAP -0.886168896864937 3.20981795524244e-34 NA NA NA hsa-miR-9-5p PRLR -0.125377971246422 0.269896477802284 -0.090634989600836 0.414123305437059 miRNATAP -1.18446280913297 1.25226082012491e-32 NA NA NA hsa-miR-25-3p PTEN 0.316649850160985 0.000777982468387578 -0.166774236847838 0.000693239621129742 miRTarBase;MirTarget;miRNATAP -0.10796918628155 5.86307501062941e-07 NA NA NA hsa-miR-320a PTEN 0.092135599636725 0.243028758989929 -0.166774236847838 0.000693239621129742 MirTarget;PITA;miRanda;miRNATAP -0.120673517851669 1.04464750662028e-06 NA NA NA hsa-miR-320b PTEN 0.283477746304177 0.0005953763020907 -0.166774236847838 0.000693239621129742 MirTarget;PITA;miRanda;miRNATAP -0.101263900507425 6.87434169702041e-08 NA NA NA hsa-miR-93-5p PTEN 0.139766237971122 0.101057320076629 -0.166774236847838 0.000693239621129742 miRNAWalker2_validate;miRTarBase;miRNATAP -0.127367364164339 6.57333465080741e-06 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-125a-5p RAF1 -0.0833349424517387 0.325440200253607 -0.00553586733389189 0.905894621254653 miRanda -0.122061217158812 1.14719678269113e-13 NA NA NA hsa-miR-146b-3p RAF1 0.283486177568242 0.0118918188864879 -0.00553586733389189 0.905894621254653 MirTarget;PITA;miRNATAP -0.122160060310276 3.66962239698306e-36 NA NA NA hsa-miR-23b-3p RAF1 0.0476610245860094 0.630277683372344 -0.00553586733389189 0.905894621254653 miRNAWalker2_validate -0.142246538184778 8.25666529544947e-31 NA NA NA hsa-miR-484 RAF1 -0.0190828819137732 0.802298900989652 -0.00553586733389189 0.905894621254653 MirTarget -0.126030948035335 1.89321721164606e-15 NA NA NA hsa-miR-1224-5p RELA -0.53941068839383 0.00201058335769201 0.0739196932169914 0.126314845681798 mirMAP -0.105338562253176 5.76511309726817e-46 NA NA NA hsa-miR-138-5p RELA -0.616087649920851 1.35200027885729e-05 0.0739196932169914 0.126314845681798 miRNATAP -0.118615517312994 4.30114332551203e-37 NA NA NA hsa-miR-324-5p RELA 0.0183370482495953 0.842996782677271 0.0739196932169914 0.126314845681798 miRNAWalker2_validate;miRanda -0.154645221576587 4.11812534839311e-25 NA NA NA hsa-miR-330-5p RELA -0.262932095995605 0.0465226565966658 0.0739196932169914 0.126314845681798 miRanda -0.120880678711014 2.77817174522426e-33 NA NA NA hsa-miR-488-3p RELA -0.257955761012568 0.00280795162806256 0.0739196932169914 0.126314845681798 mirMAP -0.149905441066056 1.6489641903294e-20 NA NA NA hsa-miR-769-5p RELA -0.320572895263071 0.00425481410304398 0.0739196932169914 0.126314845681798 miRNATAP -0.185846508366017 6.56909022979932e-59 NA NA NA hsa-miR-940 RELA -0.0183807673806575 0.881747775878136 0.0739196932169914 0.126314845681798 mirMAP -0.130602708311796 2.82198893322046e-34 NA NA NA hsa-miR-15a-5p RELN 0.142658985373611 0.0536219185009004 -0.795637039279342 8.67966790787199e-06 MirTarget;miRNATAP -0.357288394756752 0.00548095336359057 NA NA NA hsa-miR-15b-5p RELN 0.592863156692837 1.41971517354055e-10 -0.795637039279342 8.67966790787199e-06 MirTarget;miRNATAP -0.325148936889226 0.000258962228055299 NA NA NA hsa-miR-27a-5p RELN 0.588526581446 1.44259383783551e-07 -0.795637039279342 8.67966790787199e-06 miRNATAP -0.206318188834117 0.00314521038452383 NA NA NA hsa-miR-424-5p RELN 0.25140374049676 0.00709934939774983 -0.795637039279342 8.67966790787199e-06 MirTarget;miRNATAP -0.379098086052582 1.50628125077061e-05 NA NA NA hsa-miR-92a-3p RHEB 0.0407239381334659 0.643983393805961 -0.0055020873063043 0.913980293216823 miRNAWalker2_validate -0.163147163919047 1.47617791483702e-08 NA NA NA hsa-let-7d-5p RPS6 0.111345229817909 0.0872634398336815 -0.104558379713959 0.190176886993187 miRNAWalker2_validate -0.29256917297677 8.13900552454981e-09 NA NA NA hsa-miR-15b-5p RPS6 0.592863156692837 1.41971517354055e-10 -0.104558379713959 0.190176886993187 miRNAWalker2_validate -0.165622735662723 4.45449155751687e-08 NA NA NA hsa-miR-16-5p RPS6 0.246024668671282 0.00346042804503716 -0.104558379713959 0.190176886993187 miRNAWalker2_validate -0.124575650458185 0.000970490092358014 NA NA NA hsa-miR-652-3p RPS6 -0.127169279300343 0.101103251579954 -0.104558379713959 0.190176886993187 miRNAWalker2_validate -0.142807153225984 8.67104597386709e-05 NA NA NA hsa-miR-744-5p RPS6 -0.31868579180387 0.000969037378618306 -0.104558379713959 0.190176886993187 miRNAWalker2_validate -0.167109496744133 8.46766748095566e-09 NA NA NA hsa-miR-103a-3p RPS6KB1 -0.147443193527362 0.128400914654149 0.0307883693941573 0.477549325099404 miRNAWalker2_validate -0.184910276785145 6.35307901545141e-17 NA NA NA hsa-miR-30a-5p RPS6KB1 -0.062942936611238 0.513851954700252 0.0307883693941573 0.477549325099404 mirMAP;miRNATAP -0.205770844947332 4.93072538380941e-21 NA NA NA hsa-miR-30b-5p RPS6KB1 -0.0858101016322106 0.263243418521854 0.0307883693941573 0.477549325099404 mirMAP -0.123678081322919 1.77291313740392e-09 NA NA NA hsa-miR-30c-5p RPS6KB1 -0.0469114469943097 0.565934557464627 0.0307883693941573 0.477549325099404 mirMAP;miRNATAP -0.167666457775001 1.62284709251961e-16 NA NA NA hsa-miR-342-3p RPS6KB1 -0.125683829770877 0.105810751640137 0.0307883693941573 0.477549325099404 miRanda -0.104465615578186 3.37906515464407e-08 NA NA NA hsa-miR-361-5p RPS6KB1 0.0107519600643302 0.882230614095565 0.0307883693941573 0.477549325099404 miRanda -0.127227133278008 1.38227078328191e-08 NA NA NA hsa-miR-500a-5p RPS6KB1 0.0634452729978863 0.396072382601677 0.0307883693941573 0.477549325099404 MirTarget -0.125164970220538 7.44035968565634e-13 NA NA NA hsa-miR-500b-5p RPS6KB1 0.0632030165009851 0.39791248661688 0.0307883693941573 0.477549325099404 MirTarget -0.124909608536446 8.29092310388171e-13 NA NA NA hsa-miR-664a-3p RPS6KB1 0.0413918462467198 0.611334357897022 0.0307883693941573 0.477549325099404 MirTarget -0.137323042284789 2.51680554045394e-17 NA NA NA hsa-miR-98-5p RPS6KB1 -0.0806971683871618 0.251777941211683 0.0307883693941573 0.477549325099404 miRNAWalker2_validate -0.119576545684213 1.48092849970582e-08 NA NA NA hsa-miR-331-3p RPS6KB2 0.0126984726684189 0.905175053956974 0.0714790859004983 0.127724239330154 miRNATAP -0.154713525602994 9.99275127049366e-21 NA NA NA hsa-miR-423-3p RXRA 0.192317829125882 0.00125622290742326 -0.149677764384537 0.00620173347653026 miRNAWalker2_validate -0.147545022065775 5.63375275497137e-05 NA NA NA hsa-miR-450b-5p SGK3 0.270544879013992 0.00801537838499562 -0.169308011680584 0.0186134859798665 PITA;mirMAP;miRNATAP -0.104169949050845 0.000313297797939948 NA NA NA hsa-miR-96-5p SGK3 -0.217255611810229 0.209932800103896 -0.169308011680584 0.0186134859798665 miRNAWalker2_validate;TargetScan -0.103739740183726 5.05443316849729e-10 NA NA NA hsa-miR-22-5p SOS1 0.0437233965539527 0.639996108995995 0.00523748905885846 0.912745973623718 miRNATAP -0.124027312186166 1.41352250447066e-15 NA NA NA hsa-miR-30a-5p SOS1 -0.062942936611238 0.513851954700252 0.00523748905885846 0.912745973623718 mirMAP;miRNATAP -0.177492800844273 6.3171352970898e-13 NA NA NA hsa-miR-30b-5p SOS1 -0.0858101016322106 0.263243418521854 0.00523748905885846 0.912745973623718 mirMAP -0.16385231555963 4.41286025144966e-13 NA NA NA hsa-miR-30c-2-3p SOS1 -0.18630560418806 0.0313113706136533 0.00523748905885846 0.912745973623718 MirTarget -0.104452765489338 1.12374921832207e-08 NA NA NA hsa-miR-30c-5p SOS1 -0.0469114469943097 0.565934557464627 0.00523748905885846 0.912745973623718 mirMAP;miRNATAP -0.197561323004552 1.3758821302056e-18 NA NA NA hsa-miR-361-5p SOS1 0.0107519600643302 0.882230614095565 0.00523748905885846 0.912745973623718 miRanda -0.115873078362459 3.5425762216149e-06 NA NA NA hsa-miR-365a-3p SOS1 -0.184222766207919 0.0292663235724171 0.00523748905885846 0.912745973623718 miRNATAP -0.149763665687287 4.3259532044635e-18 NA NA NA hsa-miR-664a-3p SOS1 0.0413918462467198 0.611334357897022 0.00523748905885846 0.912745973623718 mirMAP -0.132986198696247 2.29333790618546e-13 NA NA NA hsa-miR-146b-5p SOS2 0.441078059667297 0.000237619929889385 -0.136173831265531 0.0089381592916821 miRanda -0.155137029146937 3.46260583915288e-27 NA NA NA hsa-miR-15a-5p SOS2 0.142658985373611 0.0536219185009004 -0.136173831265531 0.0089381592916821 miRNATAP -0.11015727851104 9.81799896258958e-05 NA NA NA hsa-miR-15b-5p SOS2 0.592863156692837 1.41971517354055e-10 -0.136173831265531 0.0089381592916821 miRNATAP -0.149377221011986 7.33700935351651e-15 NA NA NA hsa-miR-664a-3p SOS2 0.0413918462467198 0.611334357897022 -0.136173831265531 0.0089381592916821 mirMAP -0.18595244526209 1.63522338473879e-18 NA NA NA hsa-miR-139-5p SPP1 -0.647754756897633 2.15378307237163e-06 0.447976109877716 0.00440666771572369 miRanda -0.244822467822024 1.08036600087553e-06 NA NA NA hsa-miR-330-5p STK11 -0.262932095995605 0.0465226565966658 0.0323033339043022 0.501366117615011 miRanda -0.102574389360944 2.58965697812856e-19 NA NA NA hsa-miR-362-5p SYK -0.0730185557229279 0.425572997292224 0.168479442175911 0.12180293423264 mirMAP -0.140967745402866 0.00646116795793003 NA NA NA hsa-miR-767-3p SYK -0.429854368420513 0.000821445291167688 0.168479442175911 0.12180293423264 miRNATAP -0.224244300989284 8.22870530037048e-10 NA NA NA hsa-miR-92a-3p SYK 0.0407239381334659 0.643983393805961 0.168479442175911 0.12180293423264 mirMAP -0.293258462292168 0.000489612424293926 NA NA NA hsa-miR-23a-3p TGFA 0.334693010651106 0.00144448147063433 -0.189232317094824 0.055531144411115 miRNATAP -0.217827291086455 2.52845742435621e-06 NA NA NA hsa-miR-452-5p TGFA 0.599327068454211 1.02031931068531e-05 -0.189232317094824 0.055531144411115 mirMAP -0.143183435159408 4.31513936230706e-06 NA NA NA hsa-miR-455-3p TGFA 0.512701816402104 0.000459755983280155 -0.189232317094824 0.055531144411115 miRNATAP -0.13115224083861 8.27073816161446e-06 NA NA NA hsa-miR-576-5p TGFA 0.106482276040047 0.194379152564166 -0.189232317094824 0.055531144411115 mirMAP -0.279355110949944 1.13491350033482e-07 NA NA NA hsa-miR-338-3p THBS1 -0.39963704679384 0.0034410643220691 0.542687032308904 0.000869835800177595 MirTarget;PITA;miRanda -0.218145983536744 0.000122986602033708 NA NA NA hsa-miR-106b-5p THBS2 0.167096183547615 0.0497544396383451 0.100434752325962 0.25920490224938 MirTarget -0.141807984689074 0.00446283348207612 NA NA NA hsa-miR-17-5p THBS2 0.0975751369167082 0.20621110214601 0.100434752325962 0.25920490224938 MirTarget;TargetScan -0.195108446647177 0.00119192440747675 NA NA NA hsa-miR-20a-5p THBS2 -0.0115865752288853 0.887069448776018 0.100434752325962 0.25920490224938 MirTarget -0.162285632609941 0.00329046667446248 NA NA NA hsa-miR-326 THBS3 -0.631050656251074 8.83643450601471e-05 0.344232751428636 4.8416323898698e-08 PITA -0.154333676544101 9.87719126011395e-23 NA NA NA hsa-miR-330-5p THBS3 -0.262932095995605 0.0465226565966658 0.344232751428636 4.8416323898698e-08 PITA;miRNATAP -0.124727656240419 3.61411008576065e-10 NA NA NA hsa-miR-30a-5p THEM4 -0.062942936611238 0.513851954700252 -0.104574607499127 0.118115475807219 miRNAWalker2_validate -0.233970244012662 1.33116272372442e-06 NA NA NA hsa-miR-576-5p THEM4 0.106482276040047 0.194379152564166 -0.104574607499127 0.118115475807219 MirTarget -0.115862778271938 0.000956791026050953 NA NA NA hsa-miR-105-5p TLR2 -0.567459149851677 8.20858944634795e-06 0.45279823329852 0.000583640359994633 miRNAWalker2_validate;miRTarBase -0.291807523887962 9.84625895586496e-11 NA NA NA hsa-miR-146a-5p TLR4 0.148942451196282 0.114229072828724 -0.432039705165988 8.65135411254704e-05 miRNAWalker2_validate;miRTarBase -0.252255846203041 4.1723793537364e-07 NA NA NA hsa-miR-146b-5p TLR4 0.441078059667297 0.000237619929889385 -0.432039705165988 8.65135411254704e-05 miRanda -0.130320997069911 0.001388609119677 NA NA NA hsa-miR-17-5p TLR4 0.0975751369167082 0.20621110214601 -0.432039705165988 8.65135411254704e-05 TargetScan -0.262030224274721 0.000489054832919909 NA NA NA hsa-miR-129-5p TNC -0.567133990117126 0.00116152215679936 0.555137977153671 5.94265484482517e-05 miRanda -0.149509764155064 9.83486700574452e-06 NA NA NA hsa-miR-137 TNC -0.924849266740909 1.546832202049e-06 0.555137977153671 5.94265484482517e-05 PITA;miRanda -0.164545713441763 2.43213075870192e-08 NA NA NA hsa-miR-218-5p TNC -0.534287404275829 0.00308322441944796 0.555137977153671 5.94265484482517e-05 MirTarget;miRNATAP -0.142788202398406 7.00144043531709e-06 NA NA NA hsa-miR-342-3p TNN -0.125683829770877 0.105810751640137 0.279778372529743 0.0348965576205685 miRanda -0.322774505795137 0.000118188113490669 NA NA NA hsa-miR-125a-3p TNR 0.183834985432376 0.106230647107917 -0.754166527021585 0.000128792181925123 miRanda -0.355583328627218 2.5188696649431e-06 NA NA NA hsa-miR-141-3p TNR 0.41120561991992 0.000393344390969055 -0.754166527021585 0.000128792181925123 TargetScan -0.681506016189482 3.53422419274657e-21 NA NA NA hsa-miR-199a-5p TNR 0.174889610847968 0.0900027418912454 -0.754166527021585 0.000128792181925123 miRanda -0.585094832247826 1.25216168253662e-12 NA NA NA hsa-miR-199b-5p TNR 0.436298593972862 0.00200225454383871 -0.754166527021585 0.000128792181925123 miRanda -0.43908001967346 8.84012319977973e-14 NA NA NA hsa-miR-320c TNR 0.343259124189966 0.000248338401291051 -0.754166527021585 0.000128792181925123 miRanda -0.282048175567799 0.00261812386409165 NA NA NA hsa-miR-34c-5p TNR 0.181931113688093 0.257266965617363 -0.754166527021585 0.000128792181925123 miRanda -0.281467618596113 7.05525252343887e-08 NA NA NA hsa-miR-484 TNR -0.0190828819137732 0.802298900989652 -0.754166527021585 0.000128792181925123 MirTarget -0.387337928138639 0.0013063023859308 NA NA NA hsa-miR-3607-3p TNXB -0.0118040987013632 0.907228689257085 0.224142080211946 0.00626770862439315 miRNATAP -0.109390944240756 0.00121518350822448 NA NA NA hsa-miR-125a-5p TP53 -0.0833349424517387 0.325440200253607 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase;miRanda -0.375445863722298 1.2184854773952e-16 26389681;21777146;23079745 Mechanistically inactivation of miR-125a during cervical carcinogenesis was caused by HPV suppression of p53 expression;In addition wild-type p53 mRNA and protein expression was increased by hsa-miR-125a-5p overexpression; Moreover blocking wild-type p53 attenuated the effect of hsa-miR-125a-5p on apoptosis; In loss-of-function experiments wild-type p53 mRNA and protein expression was decreased by blocking hsa-miR-125a-5p; The effect of hsa-miR-125a-5p inhibitor on apoptosis was also weakened by blocking wild-type p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity tumorigenesis;; cervical and endocervical cancer;lung cancer;liver cancer hsa-miR-125b-5p TP53 -0.0119698382910496 0.890233923197358 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase -0.294628443669371 1.07324994036762e-07 24169356;24846940;26335100;24137477;26686386;24402874;21399871;23585871;24762088;23079745 MiR 125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK independent pathways; Further studies reveal that p53 is regulated by miR-125b; However downregulation of the endogenous miR-125b also results in p53-independent apoptotic pathway leading to apoptosis in p53 mutated U251 cells and p53 knockdown U87 cells;The results revealed that hsa-miR-125b may regulate multiple biological processes and signal transduction pathways and drug-resistant occurrence is associated with cell proliferation cell apoptosis cell cycle and signaling pathways including MAPK Wnt and p53;The carboxy terminal domain of connexin 43 CT Cx43 modulates the expression of p53 by altering miR 125b expression in low grade human breast cancers; In addition CT-Cx43 was exogenously expressed in the breast cancer-derived cell line MCF-7 and its effect on the expression of miR-125b and its downstream target p53 were evaluated as well as its effect on cellular proliferation and death using MTT and LDH assays respectively; Interestingly we found that miR-125b a negative regulator of p53 exhibited an inverse expression relationship with CT-Cx43 in the breast cancer samples tested;TP53 and hsa-miR-125b were observed to form a self-adaptation association;In this study we have investigated the delivery and transfection of wild-type wt- p53 and microRNA-125b miR-125b expressing plasmid DNA in SK-LU-1 human lung adenocarcinoma cells as well as in KrasG12D/p53fl/fl KP genetically engineered mouse model of lung cancer;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;In vitro assays revealed that overexpression of miR-125b repressed the endogenous level of p53 protein in human colorectal cancer cells;In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b;A stable overexpression of miR-125b in human melanoma cell line Mel-Juso resulted in a G0/G1 cell cycle block and emergence of large cells expressing senescence markers: senescence-associated beta-galactosidase p21 p27 and p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity; To show the clinical significance of these findings mutations in the DNA binding domain of p53 and promoter methylation of miR-125b were investigated; Four out of nine patients with induced SIRT7 carried mutations in the p53 gene and one patient showed hypermethylation of the miR-125b promoter region ;;;;;immune resistance;;;; glioblastoma;gastric cancer;breast cancer;pancreatic cancer;lung cancer;cervical and endocervical cancer;colorectal cancer;prostate cancer;melanoma;liver cancer hsa-miR-150-5p TP53 0.0645271721519611 0.513295658828718 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.156281214913021 3.09404029899862e-06 23747308;23228962 miR 150 promotes the proliferation of lung cancer cells by targeting P53; Here we demonstrate that miR-150 is aberrantly upregulated in lung cancer tissue and negatively correlates with the expression of the proapoptotic gene p53 but not EGR2; We show that miR-150 specifically targets the 3'-UTR of p53 and regulates its expression; Inhibition of miR-150 effectively delays cell proliferation and promotes apoptosis accompanied by increased p53 protein expression;miR-150 and miR-3940-5p were found to be significantly downregulated in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA; miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect pathway ; lung cancer;lung squamous cell cancer hsa-miR-221-3p TP53 -0.0109507285071775 0.936440693394519 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.143405645468124 6.89189438710031e-10 20880178;24324033 Circulating miR 221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression; The correlation between miR-221 levels and protein levels of p53 CEA ER and PR clinicopathological features or overall survival was analyzed; The immunohistochemistry analysis demonstrates a significant correlation between plasma miR-221 level and p53 expression; The direct amplification of plasma miR-221 can be used as a potential noninvasive molecular marker for diagnosis and prognosis of CRC and is correlated with p53 expression;Interestingly miR-221 can activate the p53/mdm2 axis by inhibiting MDM2 and in turn p53 activation contributes to miR-221 enhanced expression; Moreover by modulating the p53 axis miR-221 impacts cell-cycle progression and apoptotic response to doxorubicin in hepatocellular carcinoma-derived cell lines; These data were confirmed in clinical specimens of hepatocellular carcinoma in which elevated miR-221 expression was associated with the simultaneous presence of wild-type p53 and DNA hypomethylation poor survival;worse prognosis;progression;drug resistance colorectal cancer;liver cancer hsa-miR-222-3p TP53 0.107117065575197 0.423818119725697 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.142653482163308 1.2671214916531e-09 NA NA NA hsa-miR-30d-5p TP53 -0.0607010868720046 0.471410043548794 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.336903694979927 3.32137008004591e-09 NA NA NA hsa-miR-324-5p TP53 0.0183370482495953 0.842996782677271 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.170645382602635 1.21583558752455e-06 NA NA NA hsa-miR-338-3p TP53 -0.39963704679384 0.0034410643220691 0.346312114104146 1.42081328137217e-05 miRanda -0.117560999445537 4.2049029410775e-06 NA NA NA hsa-miR-34a-5p TP53 0.143472919835524 0.211283228567057 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.127236433475415 7.74576992902642e-06 23155233;19773441;25572695;25123132;22438124;21702042;22292433;21383543;25982144;19443717;26790955;24642471;22198213;25362853;23036084;19421141;24444609;20186752;22457788;27186405;21731696;19736307;26879132;23569431;25771001;23292869;24209638;24957404;21240262;24503183;22102859;18803879;24630988;25895459;25894979;19714243;26944831;19921694;21909380;25789847;23450486;24402874;24337371;18497571;25490093;22593438;24528540;20145172;23349340;25038915;21321636;23632240;23624843;19029026;20309940;22810507;23862748;25932212;18834855;21225432 The high miR-34a expression level in the cells after irradiation at 60 Gy reduced the p53 expression level;MicroRNA-34a miR-34a is a transcriptional target of p53 that is down-regulated in some cancer cell lines; Analysis of human specimens showed that miR-34a expression is down-regulated in glioblastoma tissues as compared with normal brain and in mutant p53 gliomas as compared with wild-type p53 gliomas;For this purpose cell viability assay Realtime quantitative PCR for mRNA quantification western blot for essential protein expression p53 silencing by shRNA and miR-34a knockdown were performed in the present study; PRE also elevated p53 protein and triggered miR-34a expression;As expected p53 loss caused downregulation of established p53 targets e.g p21 and miR-34 family and increased proliferation in both luminal and basal-like cell lines;Moreover re-expression of miR-34a by transfection in NSCLC cells resulted in inhibition of cell growth and invasiveness induction of apoptosis and enhanced p53 activity;MiR 34a chemosensitizes bladder cancer cells to cisplatin treatment regardless of p53 Rb pathway status; MiR-34a is a downstream effector of p53 that has been shown to target several molecules associated with cell cycle and cell survival pathways; As alterations in these pathways are frequent in muscle invasive transitional cell carcinoma of the bladder MI-TCC for example mutation or loss of p53 and Rb the goal of this study was to determine whether manipulation of miR-34a expression levels could abrogate the effect of these alterations and sensitize bladder cancer cells to chemotherapy;Reporter assay further showed that NF-kappaB-induced miR-34a transcriptional activity was reduced by p53 impairment; And wildtype p53 is responsible for NF-kappaB-mediated miR-34a transcriptional activity but not for NF-kappaB binding;Recently miR-34 family has been shown to be part of the p53 pathway which is frequently involved in lung cancer and the expression of miR-34 has been reported to be regulated by DNA methylation;5 Aminolevulinic acid mediated sonodynamic therapy induces anti tumor effects in malignant melanoma via p53 miR 34a Sirt1 axis; Therefore the p53 miR-34a and SIRT1 constituted a positive feedback loop;miR-34a expression could be increased in Y79 cells but not Weri-Rb1 cells after p53 activation; This differential regulation was not caused by genomic alterations at the miR-34a p53 binding site or mature gene;Conversely kallistatin stimulated expression of the tumorigenic suppressors miR-34a and p53;Members of the miR-34 family are induced by the tumor suppressor p53 and are known to inhibit epithelial-to-mesenchymal transition EMT and therefore presumably suppress the early phases of metastasis;MicroRNA-34a miR-34a a transcriptional target of p53 is a well-known tumor suppressor gene; Moreover we found that miR-34a was downregulated in 25 of 40 62.5% colon cancer tissues as compared with the adjacent normal colon tissues and that the expression of miR-34a was correlated with the DNA-binding activity of p53;Ectopic expression of miR-34a-5p in p53 wild-type colon cancer cell HCT116 significantly inhibited cell growth migration invasion and metastasis; miR-34a-5p induced cell apoptosis cell cycle arrest at G1 phase and p53 transcription activity in HCT116 cells but not in the HCT116 p53 knockout p53-/- cells; miR-34a-5p significantly suppressed the HCT116 growth in vivo whereas it showed no effect on the HCT116 p53-/- xenograft indicating that the growth-inhibiting effect by miR-34a-5p was dependent on p53;microRNA 34a sensitizes lung cancer cell lines to DDP treatment independent of p53 status; However the precise biological role of miR-34a in p53 deficient lung cancer cell lines remains largely elusive; Overall in this study we found the proliferation inhibition function of miR-34a in vitro in lung cancer cell lines is p53 independent and also demonstrated the combination therapeutic potential of miR-34a and DDP in lung cancer cell lines;In particular mammalian miR-34 is upregulated by p53 in response to radiation but little is known about the role of this miRNA in vivo; These findings show a role for mir-34 in both apoptotic and non-apoptotic cell death in vivo much like that of cep-1 the C elegans p53 homolog;Tumor-suppressive miR-34a a direct target of p53 has been shown to target several molecules of cell survival pathways; Here we show that capsaicin-induced oxidative DNA damage culminates in p53 activation to up-regulate expression of miR-34a in non-small cell lung carcinoma NSCLC cells;Accumulating evidence suggests that miR-34a as a key mediator of p53 tumor suppression is aberrantly expressed in human cancers; Bioinformatics analysis produced a protein-protein interaction network which revealed that the p53 signaling pathway and cell cycle pathway were two major hubs containing most of the proteins regulated by miR-34a;Studies have demonstrated that miR-34a which is a direct target of the p53 tumor suppressor gene functions as a tumor suppressor and is associated with the tumor growth and metastasis of various human malignances;Knockdown of oncoprotein E6 expression of human papillomavirus in SiHa and HeLa cells by siRNAs lead to an increased protein level of p53 decreased level of miR-34a as well as reduced Warburg effect;miR-34a is known as a p53 regulated tumor suppressor microRNA in many cancer types;In addition in patients with sufficient tumor tissue we assessed p53 mutations and the methylation status of the MIRN34A gene promoter region and correlated these findings with miR-34a expression; Patients with both p53 mutations and low miR-34a levels had the highest probability of relapse P = 0.001;Particularly miR-34a has been revealed to be a direct transcriptional target of p53 which is frequently mutated in epithelial ovarian carcinomas especially in high grade serous cancer; The aim of this study was to investigate the clinical relevance of mir34a as well as its promoter methylation in a subset of 133 ovarian cancers with a special focus on the p53 mutation status the dualistic type I and type II ovarian cancer model and the different histotypes; The expression of miR-34a was found lower in type II than in type I cancers p = 0.037 in p53 mutated as compared to p53 wild type cancers p = 0.003 and in high grade compared to in low grade cancers p = 0.028; The inverse association between miR-34a expression and grading p53 mutation status and dualistic tumor type classification together with its prognostic relevance may underline the tumor-suppressive character of miR-34a in ovarian cancer;As a direct target gene of p53 miR-34a has been suggested to mediate the tumor suppressor function of p53;Further studies indicated that PEG4000-TQ-Nps could significantly increase the expression of miR-34a through p53;Downregulation of miR 34a in breast tumors is not associated with either p53 mutations or promoter hypermethylation while it correlates with metastasis; Recent studies have shown that p53 upregulates miR-34 family leading to direct repression of several key oncogenes; In this study expression of mature miR-34a in breast tumors with wild-type p53 was investigated in order to find any correlation between dysregulation of miR-34a expression and breast cancer; In about 40 % of the wild-type p53 samples miR-34a was significantly downregulated; This study has provided evidence that miR-34a expression can be affected in a significant proportion of breast tumors independent of p53; Knowledge of miR-34a status may provide additional useful information regarding the nature of breast tumors especially when p53 testing does not show any aberration;Among these we found well studied molecules such as the miR-17-92 cluster comprising potent oncogenic microRNA and miR-34 recently found to interact with p53;The results demonstrated that miR-199a and miR-34a could induce the apoptosis of human osteosarcoma cells via p53 signalling pathway;Here we show through expression analysis that miR-34a a p53 target was underexpressed in CD44+ prostate cancer cells purified from xenograft and primary tumors;Members of the miR-34 family have been shown to be transcriptional targets of the tumour suppressor gene P53;Functional genomic analysis highlighted a novel regulatory role of the transcription factor MAZ apart from the known control by p53 on the expression of miR-34a and a number of miR-34a targets; Our analysis for regulatory loops suggest that MAZ and p53 transcription factors co-operate in modulating miR-34a as well as miR-34a targets involved in several cellular pathways;Restoration of tumor suppressor miR 34 inhibits human p53 mutant gastric cancer tumorspheres; MicroRNA miR-34 was recently found to be a direct target of p53 functioning downstream of the p53 pathway as a tumor suppressor; Our results demonstrate that in p53-deficient human gastric cancer cells restoration of functional miR-34 inhibits cell growth and induces chemosensitization and apoptosis indicating that miR-34 may restore p53 function;miR 34 cooperates with p53 in suppression of prostate cancer by joint regulation of stem cell compartment; The miR-34 family was originally found to be a direct target of p53 and is a group of putative tumor suppressors; Here we report that mice with prostate epithelium-specific inactivation of mir-34 and p53 show expansion of the prostate stem cell compartment and develop early invasive adenocarcinomas and high-grade prostatic intraepithelial neoplasia whereas no such lesions are observed after inactivation of either the mir-34 or p53 genes alone by 15 months of age; Consistently combined deficiency of p53 and miR-34 leads to acceleration of MET-dependent growth self-renewal and motility of prostate stem/progenitor cells; Our study provides direct genetic evidence that mir-34 genes are bona fide tumor suppressors and identifies joint control of MET expression by p53 and miR-34 as a key component of prostate stem cell compartment regulation aberrations in which may lead to cancer;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however the effect of miR-34a on the ovarian cancer cells remains unclear;The microRNA-34 family miR-34a -34b and -34c have been reported to be tumor suppressor microRNAs miRNAs that are regulated by the TP53 and DNA hypermethylation; To elucidate the roles of miR-34 family in colon carcinogenesis miR-34a/b/c were measured in tumors and adjacent noncancerous tissues from 159 American and 113 Chinese colon cancer patients using quantitative RT-PCR and we examined associations between miR-34a/b/c expression with TNM staging cancer-specific mortality TP53 mutation status and Affymetrix microarray data;Transcription of the three miRNA miR-34 family members was recently found to be directly regulated by p53; Our results demonstrate that miR-34 may restore at least in part the tumor suppressing function of the p53 in p53-deficient human pancreatic cancer cells;p53 status was not correlated with miR-34a;The above results suggest that microRNA-34a is one of the important components of PRIMA-1-induced apoptotic network in the cancer cells harboring mutant p53;MicroRNA-34a miR-34a is a transcriptional target of p53 and is down-regulated in pancreatic cancer;Phytochemical regulation of the tumor suppressive microRNA miR 34a by p53 dependent and independent responses in human breast cancer cells; The tumor suppressive microRNA miR-34a is transcriptionally regulated by p53 and shown to inhibit breast cancer cell proliferation as well as being a marker of increased disease free survival; Human breast cancer cells expressing wild-type MCF-7 or mutant p53 T47D were treated with a concentration range and time course of each phytochemical under conditions of cell cycle arrest as detected by flow cytometry to examine the potential connection between miR-34a expression and their anti-proliferative responses; Our results suggest that miR-34a is an essential component of the anti-proliferative activities of I3C artemisinin and artesunate and demonstrate that both wild-type p53 dependent and independent pathways are responsible for miR-34a induction;Compared with p53 normal group the expression level of miR-34a was significantly lower in p53 deletion group;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;The microRNA miR-34 family is a direct transcriptional target of tumor-suppressor TP53 and loss of miR-34 function may impair TP53-mediated cell cycle arrest and apoptosis;It appears that AR-dependent inhibition of p53 resulted in suppression of miR-34a and -34c expression;In this study we evaluated the cascade of events determined by etoposide-induced DNA damage in OS cell lines with different p53 status focusing on methylation status and expression of miR-34a that modulate tumor cell growth and cell cycle progression; Our results suggest that the open and unmethylated conformation of the miR-34a gene may be regulated by p53 able to bind the gene promoter;The miR-34 family under-expressed in-non small cell lung cancers NSCLCs are effectors of p53 activation upon irradiation of cells;MiR-34a a direct p53 target gene possesses tumor-suppressive properties as they mediate apoptosis cell cycle arrest and senescence;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in human epithelial ovarian cancer EOC; miR-34a expression is decreased in 100% and miR-34b*/c in 72% of EOC with p53 mutation whereas miR-34a is also downregulated in 93% of tumors with wild-type p53; Finally miR-34 reconstitution experiments in p53 mutant EOC cells resulted in reduced proliferation motility and invasion the latter of which was dependent on MET expression;miR-34a is transcriptionally induced by the tumor suppressor gene p53 which is often downregulated in non-small cell lung cancer NSCLC;In conclusion overexpression of PEBP4 reduced the sensitivity of A549 cells to DDP-induced cytotoxicity mainly through the altered expression of the p53 protein or the modulation of miR-34a;To investigate the effects of miR-449 and miR-34 on cell growth cell cycle and target gene expression based on these miRNA different expressions in ovarian cancer cell lines SKOV3 and SKOV3-ipl both with mutation of p53;Numerous studies have focused on the association between miR-34 family members which are direct p53 targets and carcinogenesis of many cancers including hepatocellular carcinoma HCC;p53 regulates nuclear GSK 3 levels through miR 34 mediated Axin2 suppression in colorectal cancer cells;MicroRNA-34a miR-34a a potential key effector of the p53 tumor-suppressor gene was studied as a potential tumor suppressor in uveal melanoma;The miR-34 family members are direct transcriptional targets of tumor suppressor p53 and loss of miR-34 function can impair p53-mediated cell cycle arrest and apoptosis;Profiling microRNA miRNA expression delineated TP53 alteration-associated miRNA profiles and identified miR-34a and miR-100 as the most significantly down- and upregulated miRNA respectively; Clinically low miR-34a expression and TP53 alterations predicted for chemotherapy resistance and inferior outcome; Thus detailed molecular profiling links impaired p53 to decreased miR-34a expression but also identifies p53-independent miR-34a induction mechanisms as shown in TP53biallelic altered cell lines treated with 15-deoxy-Δ1214-prostaglandin;MiR-34a a direct target of p53 has been shown to target several molecules associated with the cell cycle and cell survival pathways and its dysregulation is implicated in cancer drug resistance or sensitivity in several human cancers;MicroRNA-34a miR-34a is a direct transcriptional target of p53 and links tumor suppressor function and the oncogenic pathways in some cancers;Tumor suppressor p53 transcriptionally regulates expression of microRNA-34a which confers translational inhibition and mRNA degradation of genes involved in cell cycle control and apoptosis; MiR-34a expression was markedly reduced in p53-null PC3 cells and p53-mutated DU145 cells compared with LNCaP cells expressing wild-type p53;The microRNA encoding genes miR-34a and miR-34b/c represent direct p53 target genes and possess tumor suppressive properties as they mediate apoptosis cell cycle arrest and senescence; In the colorectal cancer samples a statistically significant correlation of miR-34a methylation and the absence of p53 mutation was detected; The mutual exclusiveness of miR-34a methylation and p53 mutation indicates that miR-34a inactivation may substitute for loss of p53 function in cancer ;;;;;poor survival;;;malignant trasformation;;;metastasis;;metastasis;;drug resistance;poor survival;;metastasis;;;;;;;metastasis;;;;;;;motility;;staging;tumorigenesis;;;;;drug resistance;poor survival;;immune resistance;;;progression;;;motility;;;;tumorigenesis;;;;drug resistance;poor survival;drug resistance;;; glioblastoma;glioblastoma;bladder cancer;breast cancer;lung squamous cell cancer;bladder cancer;esophageal cancer;lung squamous cell cancer;melanoma;retinoblastoma;breast cancer;colorectal cancer;colon cancer;colorectal cancer;lung cancer;breast cancer;lung squamous cell cancer;liver cancer;sarcoma;cervical and endocervical cancer;lung squamous cell cancer;lung squamous cell cancer;ovarian cancer;sarcoma;breast cancer;breast cancer;head and neck cancer;sarcoma;prostate cancer;kidney renal cell cancer;breast cancer;gastric cancer;prostate cancer;ovarian cancer;colon cancer;pancreatic cancer;esophageal cancer;lung cancer;pancreatic cancer;breast cancer;B cell lymphoma;cervical and endocervical cancer;colorectal cancer;prostate cancer;sarcoma;lung squamous cell cancer;esophageal cancer;ovarian cancer;lung squamous cell cancer;lung cancer;ovarian cancer;liver cancer;colorectal cancer;melanoma;liver cancer;acute myeloid leukemia;liver cancer;gastric cancer;prostate cancer;sarcoma hsa-miR-380-5p TP53 -0.356387125389103 0.0163515117264043 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.130032370502874 2.53621800466177e-09 NA NA NA hsa-miR-381-3p TP53 -0.192118086401438 0.203198554750071 0.346312114104146 1.42081328137217e-05 MirTarget -0.10958470195975 2.36383687034549e-07 NA NA NA hsa-miR-421 TP53 -0.171573235029879 0.0518112933651029 0.346312114104146 1.42081328137217e-05 miRanda -0.12756767272718 0.000455424352559313 NA NA NA hsa-miR-491-5p TP53 -0.749881113074983 8.22859263179194e-09 0.346312114104146 1.42081328137217e-05 MirTarget;miRanda -0.210461133541589 1.59624493209635e-19 23519249 Targeted site prediction indicated that both Bcl-XL and TP53 contain miR-491-5p recognizing sites in their 3' UTRs; Overexpression of miR-491-5p in the pancreatic cancer cell line SW1990 effectively inhibited both endogenous Bcl-XL and TP53 gene expressions pancreatic cancer hsa-miR-504-5p TP53 -0.510726779625117 6.12122269907368e-05 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;MirTarget -0.175599590135581 1.78517568542536e-12 25015107 TFF1 activates p53 through down regulation of miR 504 in gastric cancer; Alternatively we found that the reconstitution of TFF1 down-regulates miR-504 a negative regulator of p53; Western blot analysis data demonstrated that miR-504 abrogates TFF1-induced p53 protein expression and activity; In conclusion the in vitro and in vivo data demonstrate for the first time a novel mechanism by which the tumor suppressor functions of TFF1 involve activation of p53 through down-regulation of miR-504 gastric cancer hsa-miR-142-3p TSC1 0.558908907203977 1.37828834702416e-05 -0.192441231327778 7.40107573947225e-05 miRanda -0.111374073743323 4.64908845227268e-20 NA NA NA hsa-miR-155-5p TSC1 0.969837380981085 5.86551495710056e-15 -0.192441231327778 7.40107573947225e-05 mirMAP -0.164123084015485 2.27180144621668e-48 NA NA NA hsa-miR-186-5p TSC1 0.0606381894130505 0.514217717592464 -0.192441231327778 7.40107573947225e-05 mirMAP -0.108705911736173 3.84055481394815e-09 NA NA NA hsa-miR-193a-5p TSC1 0.183172152450491 0.0749251910521214 -0.192441231327778 7.40107573947225e-05 mirMAP -0.119198790777518 1.04060760014094e-14 NA NA NA hsa-miR-200c-3p TSC1 0.413752622631695 8.6936333715621e-06 -0.192441231327778 7.40107573947225e-05 mirMAP -0.115129703586264 3.51933111264652e-12 NA NA NA hsa-miR-2355-3p TSC1 0.186207523505296 0.0525918026993012 -0.192441231327778 7.40107573947225e-05 mirMAP -0.102591805644991 1.75682794703603e-10 NA NA NA hsa-miR-23a-3p TSC1 0.334693010651106 0.00144448147063433 -0.192441231327778 7.40107573947225e-05 mirMAP -0.140801821334164 1.75892233728941e-17 NA NA NA hsa-miR-28-3p TSC1 0.139444423971018 0.0811187936164314 -0.192441231327778 7.40107573947225e-05 PITA -0.17094524054426 9.41944981479638e-12 NA NA NA hsa-miR-320c TSC1 0.343259124189966 0.000248338401291051 -0.192441231327778 7.40107573947225e-05 miRanda -0.101630640797968 2.41288752485634e-09 NA NA NA hsa-miR-339-5p TSC1 0.273253751377152 0.0185300170790638 -0.192441231327778 7.40107573947225e-05 miRanda -0.123936558418944 2.19962900066711e-21 NA NA NA hsa-miR-500a-5p TSC1 0.0634452729978863 0.396072382601677 -0.192441231327778 7.40107573947225e-05 mirMAP -0.143381569080533 5.42935555808731e-12 NA NA NA hsa-miR-501-5p TSC1 0.0878951859817452 0.309663377222443 -0.192441231327778 7.40107573947225e-05 mirMAP -0.123769893215903 5.45408040105992e-12 NA NA NA hsa-miR-576-5p TSC1 0.106482276040047 0.194379152564166 -0.192441231327778 7.40107573947225e-05 mirMAP -0.118422194307578 6.7824345548354e-10 NA NA NA hsa-miR-590-3p TSC1 0.312951515438396 0.000480455423843996 -0.192441231327778 7.40107573947225e-05 miRanda -0.109468926098831 2.4056830951107e-10 NA NA NA hsa-miR-101-3p VEGFA -0.132333931567244 0.241891922291381 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate -0.179012476724308 0.000221850261733319 NA NA NA hsa-miR-1224-5p VEGFA -0.53941068839383 0.00201058335769201 0.545202702414815 3.65580722751043e-08 MirTarget -0.12693052777853 1.1909229874546e-07 NA NA NA hsa-miR-129-5p VEGFA -0.567133990117126 0.00116152215679936 0.545202702414815 3.65580722751043e-08 miRanda -0.178350435603243 1.58281485759764e-13 NA NA NA hsa-miR-26b-3p VEGFA -0.0542537787460029 0.4802214385739 0.545202702414815 3.65580722751043e-08 MirTarget -0.151139595719221 0.00815352929658283 NA NA NA hsa-miR-29a-3p VEGFA -0.224140851576083 0.0349516103550236 0.545202702414815 3.65580722751043e-08 MirTarget -0.188400523607611 0.000280773283001581 24209632 miR 29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF A; Furthermore western blot combined with the luciferase reporter assays demonstrate that vascular endothelial growth factor A VEGF-A is direct target of miR-29a; This is the first time miR-29a was found to suppress the tumor microvessel density in gastric cancer by targeting VEGF-A gastric cancer hsa-miR-29b-2-5p VEGFA -0.26834261955727 0.00453760540520233 0.545202702414815 3.65580722751043e-08 MirTarget -0.238273224497899 1.35712590368669e-07 NA NA NA hsa-miR-29b-3p VEGFA -0.178522712044497 0.0856106432165465 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate;miRTarBase;MirTarget -0.174785884873336 0.000121514677714241 26155940;22815235 Moreover miR-29b inhibits angiogenesis by attenuating tube formation and the expression of VEGF and Ang-2 and stemness maintenance in GBM cells as demonstrated by decreasing neurosphere formation and cancer stem cell marker protein expression;Additionally for two genes that are deregulated in PCa heterogeneous nuclear ribonucleoprotein K hnRNP-K and vascular endothelial growth factor A VEGF-A we identified two regulatory miRNAs miR-205 and miR-29b ; glioblastoma;prostate cancer hsa-miR-29c-3p VEGFA -0.372180390387259 0.00202634431973208 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate;MirTarget -0.163350888739243 2.07703033697715e-05 NA NA NA hsa-miR-330-3p VEGFA -0.427388106938151 0.00300489284414266 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate -0.14940180557451 2.38610841931945e-07 NA NA NA hsa-miR-342-3p VEGFA -0.125683829770877 0.105810751640137 0.545202702414815 3.65580722751043e-08 miRanda;miRNATAP -0.183375082823802 0.00268020639799555 23408138 Statistically significant associations were observed between miR-342 and ER HER2 and vascular endothelial growth factor VEGF status in the human breast cancer samples and the levels of miR-342 gradually increased as ERα mRNA expression increased breast cancer hsa-miR-374b-5p VEGFA -0.210113949579608 0.0056866083818614 0.545202702414815 3.65580722751043e-08 MirTarget -0.259691752964477 3.6506936798186e-05 NA NA NA hsa-miR-383-5p VEGFA -0.850440167826561 1.6745326125383e-07 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate;miRTarBase;MirTarget -0.171548247272876 2.39831482676198e-11 NA NA NA hsa-miR-497-5p VEGFA -0.000783764887886562 0.991703566618871 0.545202702414815 3.65580722751043e-08 MirTarget -0.217975385158274 0.000295143152499975 26718330;26485685;26336827;26202364;27015364;25176450;26840372 Furthermore western blot assay confirmed that the overexpression of miR-497 reduced VEGF and HIF-1α protein levels; Moreover miR-497 inhibited the growth of tumors and reduced angiogenesis in a nude mouse xenograft tumor model which was probably caused by the downregulation of pro-angiogenic molecules such as VEGF and HIF-1α;Role of miR 497 in VEGF A mediated cancer cell growth and invasion in non small cell lung cancer; Here we showed that compared to other cancers NSCLC had a significant higher ratio of VEGF-A protein vs mRNA and significantly lower levels of miR-497 suggesting presence of a post-transcriptional control of VEGF-A in NSCLC different from other cancers; Compared with paired normal lung tissue NSCLC expressed lower levels of miR-497 and higher levels of VEGF-A; Moreover the levels of miR-497 and VEGF-A were inversely correlated in NSCLC specimen; Bioinformatics analyses showed that miR-497 bound to 3'-UTR of VEGF-A mRNA in NSCLC lines to inhibit its translation; Overexpression of miR-497 in NSCLC lines decreased VEGF-A protein while depletion of miR-497 in NSCLC lines increased VEGF-A protein; However modification of miR-497 levels in NSCLC lines did not alter VEGF-A mRNA levels;MiR 497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG 1; Subsequent investigations disclosed that miR-497 directly inhibited the 3'-untranslated regions UTRs of vascular endothelial growth factor A VEGFA and astrocyte elevated gene-1 AEG-1;The effects of ectopic miR-497 expression on the expression of VEGFA Akt and p-Akt were determined by western blot; Luciferase reporter assay and western blot confirmed that VEGFA was a direct target of miR-497;Subsequent investigations disclosed that Twist was regulated by miR-497 directly leading to the increased level of Vascular Endothelial Growth Factor-A VEGFA;We further disclosed that miR-497 exerted its function of anti-angiogenesis by suppressing VEGFA expression in ovarian cancer cells and in turn impairing the VEGFR2-mediated PI3K/AKT and MAPK/ERK pathways;Vascular endothelial growth factor-A VEGF-A was confirmed to be a target of miR-497 ;;metastasis;;;; breast cancer;lung squamous cell cancer;liver cancer;sarcoma;pancreatic cancer;ovarian cancer;colorectal cancer hsa-miR-504-5p VEGFA -0.510726779625117 6.12122269907368e-05 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate;miRTarBase -0.212758235683993 1.16548576784258e-10 NA NA NA hsa-miR-9-5p VEGFA -0.125377971246422 0.269896477802284 0.545202702414815 3.65580722751043e-08 miRNAWalker2_validate -0.372852027573008 5.51606960459938e-05 NA NA NA hsa-miR-140-5p VEGFB 0.065063507103468 0.380452509211773 -0.0480203658065861 0.445525895152113 miRanda -0.100656379111656 0.00277375404658955 NA NA NA hsa-miR-320a YWHAG 0.092135599636725 0.243028758989929 -0.0830248224234289 0.29162004243461 PITA;miRanda;miRNATAP -0.199400054484365 9.98164764271041e-06 NA NA NA hsa-miR-320c YWHAG 0.343259124189966 0.000248338401291051 -0.0830248224234289 0.29162004243461 PITA;miRanda;mirMAP;miRNATAP -0.137906277834443 9.70409559717807e-06 NA NA NA hsa-miR-500a-5p YWHAG 0.0634452729978863 0.396072382601677 -0.0830248224234289 0.29162004243461 MirTarget;miRNATAP -0.10181422496234 0.00820391735977638 NA NA NA hsa-miR-320a YWHAH 0.092135599636725 0.243028758989929 -0.0972709573045032 0.288940532234666 MirTarget;PITA;miRanda -0.254691930624819 1.00171465302225e-05 NA NA NA hsa-miR-320c YWHAH 0.343259124189966 0.000248338401291051 -0.0972709573045032 0.288940532234666 MirTarget;PITA;miRanda -0.134544326840746 0.000765166401614011 NA NA NA hsa-miR-9-3p YWHAH -0.184945845984695 0.0290051908009009 -0.0972709573045032 0.288940532234666 MirTarget -0.234786793332634 5.94557089030069e-05 NA NA NA