miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-105-5p AKT2 -0.567459149851677 8.20858944634795e-06 0.139361896216647 0.0151763675439579 mirMAP -0.11517777162873 2.5456118337521e-14 NA NA NA hsa-miR-146b-5p AKT3 0.441078059667297 0.000237619929889385 -0.235109681312394 0.000861339724103604 miRNAWalker2_validate -0.14853390000962 1.54850741316524e-10 NA NA NA hsa-miR-15a-5p AKT3 0.142658985373611 0.0536219185009004 -0.235109681312394 0.000861339724103604 miRNAWalker2_validate;miRTarBase;miRNATAP -0.171093255137198 0.000104922528677221 NA NA NA hsa-miR-15b-5p AKT3 0.592863156692837 1.41971517354055e-10 -0.235109681312394 0.000861339724103604 miRNATAP -0.149023414077877 1.00397425898265e-06 NA NA NA hsa-miR-28-3p AKT3 0.139444423971018 0.0811187936164314 -0.235109681312394 0.000861339724103604 miRNATAP -0.281326919526608 6.94030005162363e-11 NA NA NA hsa-miR-320a AKT3 0.092135599636725 0.243028758989929 -0.235109681312394 0.000861339724103604 PITA;miRanda;miRNATAP -0.162091233103736 0.000150010109497495 NA NA NA hsa-miR-320c AKT3 0.343259124189966 0.000248338401291051 -0.235109681312394 0.000861339724103604 PITA;miRanda;miRNATAP -0.11345587888064 0.000121683825333423 NA NA NA hsa-miR-362-3p AKT3 0.032375035235532 0.736465332563774 -0.235109681312394 0.000861339724103604 miRanda -0.133145359057788 1.93504628679194e-06 NA NA NA hsa-miR-362-5p AKT3 -0.0730185557229279 0.425572997292224 -0.235109681312394 0.000861339724103604 PITA;TargetScan;miRNATAP -0.12269062278053 4.12753291036148e-05 NA NA NA hsa-miR-501-3p AKT3 0.0726267165568464 0.382729320534971 -0.235109681312394 0.000861339724103604 miRNATAP -0.230986821283872 3.52473505159126e-12 NA NA NA hsa-miR-502-3p AKT3 0.0276791089364088 0.697605011368848 -0.235109681312394 0.000861339724103604 miRNATAP -0.217874003496912 1.14360007951059e-08 NA NA NA hsa-miR-93-5p AKT3 0.139766237971122 0.101057320076629 -0.235109681312394 0.000861339724103604 miRNATAP -0.208262975522215 1.91431764461584e-05 NA NA NA hsa-miR-125a-5p ATM -0.0833349424517387 0.325440200253607 -0.0447507082085021 0.439654845969846 miRanda -0.191891258396068 1.08085791159268e-10 NA NA NA hsa-miR-146b-5p ATM 0.441078059667297 0.000237619929889385 -0.0447507082085021 0.439654845969846 miRanda -0.139090363114708 3.02172223504569e-15 27602131 The role of microRNA 146b miR-146b in ATC remains to be elucidated; In order to characterize the role of miR-146b in ATC overexpression or interference of miR-146b was induced in ATC cell lines and cell proliferation and migration were evaluated; The potential targets of miR-146b were searched in the Gene Expression Omnibus database for ATC and matched non-tumor control samples; The expression level of potential targets was detected following overexpression or interference of miR-146b in ATC cell lines; In addition cell migration of ATC was also affected by miR-146b; During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis; In conclusion p21 may participate in the regulation of ATC cell proliferation by miR-146b cell migration;progression thyroid cancer hsa-miR-186-5p ATM 0.0606381894130505 0.514217717592464 -0.0447507082085021 0.439654845969846 mirMAP -0.123208669272272 4.47711357729956e-07 NA NA NA hsa-miR-18a-5p ATM 0.175320895022849 0.0732549187136286 -0.0447507082085021 0.439654845969846 miRNAWalker2_validate;miRTarBase;MirTarget -0.104121682157558 6.75084520132396e-07 23437304;25963391;23857602;23229340 MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression colorectal cancer;cervical and endocervical cancer;breast cancer;colon cancer hsa-miR-26b-5p ATM -0.0984536538762768 0.231803769574623 -0.0447507082085021 0.439654845969846 MirTarget -0.123652362350772 3.89261799231929e-05 NA NA NA hsa-miR-30b-5p ATM -0.0858101016322106 0.263243418521854 -0.0447507082085021 0.439654845969846 mirMAP -0.145142243457673 7.69838249932177e-06 25202329 However specific miRNAs are downregulated in ATC such as those of the miR-200 and miR-30 families which are important negative regulators of cell migration invasion and epithelial-to-mesenchymal transition EMT processes that are overactivated in ATC cell migration thyroid cancer hsa-miR-30c-5p ATM -0.0469114469943097 0.565934557464627 -0.0447507082085021 0.439654845969846 mirMAP -0.21095555767297 5.90764153731747e-11 NA NA NA hsa-miR-30d-3p ATM 0.0854049347792898 0.20691254801121 -0.0447507082085021 0.439654845969846 mirMAP -0.108465899695928 0.000445378990366815 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-30d-5p ATM -0.0607010868720046 0.471410043548794 -0.0447507082085021 0.439654845969846 mirMAP -0.1256777304023 0.000751902317966877 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-324-5p ATM 0.0183370482495953 0.842996782677271 -0.0447507082085021 0.439654845969846 miRanda -0.136864230463836 1.45860904388815e-09 NA NA NA hsa-miR-339-5p ATM 0.273253751377152 0.0185300170790638 -0.0447507082085021 0.439654845969846 miRanda -0.10715400072152 1.20256225486914e-09 NA NA NA hsa-miR-500a-5p ATM 0.0634452729978863 0.396072382601677 -0.0447507082085021 0.439654845969846 mirMAP -0.13339851803771 1.47786050929078e-06 NA NA NA hsa-miR-766-3p ATM -0.147747825439875 0.266658981351507 -0.0447507082085021 0.439654845969846 MirTarget -0.100027991553697 4.5702698390424e-11 NA NA NA hsa-miR-346 BAX -0.577786425963268 2.10883270131593e-09 0.353229927783273 4.30723325351116e-09 PITA;miRanda;miRNATAP -0.173817100628184 4.61458517988793e-13 NA NA NA hsa-miR-504-5p BAX -0.510726779625117 6.12122269907368e-05 0.353229927783273 4.30723325351116e-09 miRNAWalker2_validate -0.168060346140926 3.36340342595628e-20 NA NA NA hsa-miR-15a-5p BCL2 0.142658985373611 0.0536219185009004 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.15185736460016 0.000524610161257898 25594541;26915294;18931683;25623762;22335947 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MicroRNAs miRNAs are noncoding small RNAs that repress protein translation by targeting specific messenger RNAs miR-15a and miR-16-1 act as putative tumor suppressors by targeting the oncogene BCL2;miR 15a and miR 16 modulate drug resistance by targeting bcl 2 in human colon cancer cells; To investigate the reversal effect of targeted modulation of bcl-2 expression by miR-15a and miR-16 on drug resistance of human colon cancer cells;The expression of MiR-15a was significantly inhibited by Bcl-2 P < 0.05 ;poor survival;;drug resistance; breast cancer;lung cancer;prostate cancer;colon cancer;breast cancer hsa-miR-15b-5p BCL2 0.592863156692837 1.41971517354055e-10 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.15277272102586 4.15473292044312e-07 25594541;26915294;26884837;18449891 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MiR 15b mediates liver cancer cells proliferation through targeting BCL 2; MiR-15b overexpression downregulated BCL2 mRNA and protein expression obviously P < 0.05; On the contrary miR-15b inhibitor transfection markedly reduced miR-15b expression in liver cancer cells P < 0.05 promoted tumor cell proliferation and increased BCL2 mRNA and protein expression; MiR-15b can inhibit HepG2 cell proliferation and down-regulate BCL2 mRNA and protein expression;miR 15b and miR 16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells; The downregulation of miR-15b and miR-16 in SGC7901/VCR cells was concurrent with the upregulation of Bcl-2 protein; Taken together our findings suggest that miR-15b and miR-16 could play a role in the development of MDR in gastric cancer cells at least in part by modulation of apoptosis via targeting BCL2 ;poor survival;;drug resistance breast cancer;lung cancer;liver cancer;gastric cancer hsa-miR-17-5p BCL2 0.0975751369167082 0.20621110214601 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.139765906763986 0.00117394398758695 25435430 Combined overexpression of miR-16 and miR-17 greatly reduced Beclin-1 and Bcl-2 expressions respectively; miR-17 overexpression reduced cytoprotective autophagy by targeting Beclin-1 whereas overexpression of miR-16 potentiated paclitaxel induced apoptotic cell death by inhibiting anti-apoptotic protein Bcl-2 lung cancer hsa-miR-20a-5p BCL2 -0.0115865752288853 0.887069448776018 -0.188412224181801 0.00385277014382975 miRNAWalker2_validate;miRTarBase -0.191068358926279 1.1049320794207e-06 NA NA NA hsa-miR-140-5p BID 0.065063507103468 0.380452509211773 -0.303077853524202 1.77086057276777e-05 miRanda -0.226072474033958 8.47902007941316e-09 NA NA NA hsa-miR-142-3p BID 0.558908907203977 1.37828834702416e-05 -0.303077853524202 1.77086057276777e-05 MirTarget;miRanda -0.209613625216468 5.20251278351838e-24 NA NA NA hsa-miR-149-5p BID -0.0535821592029952 0.657713892012548 -0.303077853524202 1.77086057276777e-05 miRNAWalker2_validate -0.175744339411029 3.58377936748149e-15 NA NA NA hsa-miR-17-5p BID 0.0975751369167082 0.20621110214601 -0.303077853524202 1.77086057276777e-05 TargetScan -0.150049651662491 0.000572947477601046 NA NA NA hsa-miR-199a-5p BID 0.174889610847968 0.0900027418912454 -0.303077853524202 1.77086057276777e-05 miRanda -0.180779053931333 4.77962650285497e-12 NA NA NA hsa-miR-199b-5p BID 0.436298593972862 0.00200225454383871 -0.303077853524202 1.77086057276777e-05 miRanda -0.170740349718571 1.69717072548974e-20 NA NA NA hsa-miR-335-3p BID 0.34540584335595 0.00043848660222294 -0.303077853524202 1.77086057276777e-05 MirTarget -0.187737200480552 2.35088015189847e-11 NA NA NA hsa-miR-543 BID -0.347737549779625 0.0157394585079683 -0.303077853524202 1.77086057276777e-05 miRanda -0.110677529644886 5.61425667539111e-09 NA NA NA hsa-miR-592 CASP10 0.0170005651576508 0.876633091134809 0.316663771715544 5.06425951690204e-07 miRNATAP -0.197005520266077 4.49565883439056e-16 NA NA NA hsa-miR-767-3p CASP10 -0.429854368420513 0.000821445291167688 0.316663771715544 5.06425951690204e-07 mirMAP -0.10733027067527 5.92306206958344e-07 NA NA NA hsa-let-7g-5p CASP3 -0.0253396334608382 0.742556489443668 0.262751412479442 2.56855295665157e-06 MirTarget;miRNATAP -0.129202676446113 0.000422033827716308 NA NA NA hsa-miR-101-3p CASP3 -0.132333931567244 0.241891922291381 0.262751412479442 2.56855295665157e-06 MirTarget -0.106383210575799 1.67996775990553e-05 NA NA NA hsa-miR-128-3p CASP3 -0.781932174412672 7.1812756321342e-10 0.262751412479442 2.56855295665157e-06 miRNAWalker2_validate -0.154501736884318 6.74484437136043e-18 NA NA NA hsa-miR-138-5p CASP3 -0.616087649920851 1.35200027885729e-05 0.262751412479442 2.56855295665157e-06 miRNAWalker2_validate;miRTarBase -0.113189968294872 3.60467356853659e-14 NA NA NA hsa-miR-139-5p CASP3 -0.647754756897633 2.15378307237163e-06 0.262751412479442 2.56855295665157e-06 miRanda -0.14903095658868 1.69348733053664e-21 NA NA NA hsa-miR-30d-5p CASP3 -0.0607010868720046 0.471410043548794 0.262751412479442 2.56855295665157e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.147143327631089 0.000150487575452898 NA NA NA hsa-miR-342-3p CASP3 -0.125683829770877 0.105810751640137 0.262751412479442 2.56855295665157e-06 miRanda -0.139797539988921 6.72797720817884e-06 NA NA NA hsa-miR-374b-5p CASP3 -0.210113949579608 0.0056866083818614 0.262751412479442 2.56855295665157e-06 mirMAP -0.148669291364588 3.58505826678344e-06 NA NA NA hsa-miR-7-1-3p CASP3 -0.0894475316162393 0.37322359785108 0.262751412479442 2.56855295665157e-06 MirTarget -0.104664105333427 1.16615180856721e-06 NA NA NA hsa-miR-129-5p CASP6 -0.567133990117126 0.00116152215679936 0.339809506908876 1.57352977415421e-06 MirTarget;miRanda -0.125127819729221 2.27853977473712e-12 NA NA NA hsa-miR-138-5p CASP7 -0.616087649920851 1.35200027885729e-05 0.330891851472567 2.19049833744218e-07 MirTarget -0.126194182155455 4.03886338228774e-11 NA NA NA hsa-miR-488-5p CASP7 -0.385755602369049 7.99431673275965e-08 0.330891851472567 2.19049833744218e-07 MirTarget -0.148215718804746 0.000187036299869602 NA NA NA hsa-miR-9-3p CASP7 -0.184945845984695 0.0290051908009009 0.330891851472567 2.19049833744218e-07 MirTarget -0.264472424827079 1.23744649389128e-09 NA NA NA hsa-miR-105-5p CASP8 -0.567459149851677 8.20858944634795e-06 0.54677465094659 3.45072924958376e-10 MirTarget -0.327558183042417 2.59399847236527e-29 NA NA NA hsa-miR-129-5p CASP8 -0.567133990117126 0.00116152215679936 0.54677465094659 3.45072924958376e-10 miRanda -0.121355359480355 5.2366688422782e-08 NA NA NA hsa-miR-137 CASP8 -0.924849266740909 1.546832202049e-06 0.54677465094659 3.45072924958376e-10 miRanda -0.129351826753639 2.71139751772931e-11 NA NA NA hsa-miR-490-3p CASP8 -0.834268051506884 5.26203010743215e-06 0.54677465094659 3.45072924958376e-10 miRanda -0.119572674587039 1.12289344974493e-08 NA NA NA hsa-miR-125a-3p CASP9 0.183834985432376 0.106230647107917 -0.190390818122219 0.00860629060432951 miRanda -0.10922116203645 1.51947097140883e-05 NA NA NA hsa-miR-126-5p CASP9 0.177332403948664 0.0711675116243123 -0.190390818122219 0.00860629060432951 MirTarget -0.260533886267505 9.08731800375383e-19 NA NA NA hsa-miR-133a-3p CASP9 -0.335058908302964 0.0180402783121034 -0.190390818122219 0.00860629060432951 miRNAWalker2_validate;miRTarBase -0.126150713979561 1.5336707780324e-10 NA NA NA hsa-miR-140-5p CASP9 0.065063507103468 0.380452509211773 -0.190390818122219 0.00860629060432951 miRanda -0.198208812177151 1.81618262797927e-06 NA NA NA hsa-miR-15a-5p CASP9 0.142658985373611 0.0536219185009004 -0.190390818122219 0.00860629060432951 mirMAP -0.235687914842974 3.68148704667338e-07 NA NA NA hsa-miR-15b-5p CASP9 0.592863156692837 1.41971517354055e-10 -0.190390818122219 0.00860629060432951 mirMAP -0.194862381425862 1.11164072592137e-09 NA NA NA hsa-miR-16-5p CASP9 0.246024668671282 0.00346042804503716 -0.190390818122219 0.00860629060432951 mirMAP -0.203519004171527 3.05123518551225e-07 NA NA NA hsa-miR-193b-3p CASP9 0.0766333116224072 0.324723824453143 -0.190390818122219 0.00860629060432951 miRNAWalker2_validate -0.287691751817182 2.55461285152645e-14 NA NA NA hsa-miR-199a-3p CASP9 0.41226331871076 0.000497136154474528 -0.190390818122219 0.00860629060432951 mirMAP -0.203767948496481 7.25854614561419e-18 23319430 The techniques used were the MTT assay flow cytometry real-time PCR to assess miR-199a expression as also caspase-8 and caspase-9 activity in HepG2 cells treated with Propofol liver cancer hsa-miR-199b-3p CASP9 0.411848361704393 0.000517815011892379 -0.190390818122219 0.00860629060432951 mirMAP -0.202937922848071 8.40130462827375e-18 NA NA NA hsa-miR-342-5p CASP9 0.0383784541944436 0.544128449296127 -0.190390818122219 0.00860629060432951 MirTarget -0.172780651339529 0.000183272409998028 NA NA NA hsa-miR-424-5p CASP9 0.25140374049676 0.00709934939774983 -0.190390818122219 0.00860629060432951 mirMAP -0.121649518123937 0.000136932797700071 NA NA NA hsa-miR-450b-5p CASP9 0.270544879013992 0.00801537838499562 -0.190390818122219 0.00860629060432951 mirMAP -0.107405523905285 0.000148848993244956 NA NA NA hsa-miR-495-3p CASP9 -0.421982588962504 0.00504424038526901 -0.190390818122219 0.00860629060432951 mirMAP -0.112698137537794 1.81004622187323e-09 NA NA NA hsa-miR-548b-3p CASP9 0.340474544210108 0.00224491592019745 -0.190390818122219 0.00860629060432951 MirTarget -0.12601735851912 9.6237618924232e-07 NA NA NA hsa-miR-590-3p CASP9 0.312951515438396 0.000480455423843996 -0.190390818122219 0.00860629060432951 miRanda -0.109539366255286 0.000563304082980271 NA NA NA hsa-miR-346 CFLAR -0.577786425963268 2.10883270131593e-09 0.233954936864735 4.59442922000451e-06 miRNAWalker2_validate -0.110910231151455 7.37705995070254e-08 NA NA NA hsa-miR-9-5p CFLAR -0.125377971246422 0.269896477802284 0.233954936864735 4.59442922000451e-06 mirMAP -0.454877683944863 4.91513312127922e-27 NA NA NA hsa-miR-15a-5p CHUK 0.142658985373611 0.0536219185009004 -0.0805827732736795 0.0444542176915521 miRNAWalker2_validate;miRTarBase;MirTarget -0.106856768478998 8.98534595935875e-07 NA NA NA hsa-miR-15b-5p CHUK 0.592863156692837 1.41971517354055e-10 -0.0805827732736795 0.0444542176915521 MirTarget -0.133202016968096 1.16700126521113e-19 NA NA NA hsa-miR-1179 CSF2RB -0.117056451908751 0.310989525634765 0.358164957553302 0.00109646465284101 MirTarget -0.277142305322641 4.27158635401549e-11 NA NA NA hsa-miR-137 CSF2RB -0.924849266740909 1.546832202049e-06 0.358164957553302 0.00109646465284101 miRanda -0.155502720489839 1.5055922861964e-10 NA NA NA hsa-miR-3065-5p CYCS -0.262608140481507 0.0414570974148034 0.024719343035275 0.675835191929842 mirMAP -0.160124769897675 9.92708866612115e-27 NA NA NA hsa-miR-576-5p CYCS 0.106482276040047 0.194379152564166 0.024719343035275 0.675835191929842 mirMAP -0.117195910223943 2.29387377994863e-06 NA NA NA hsa-miR-93-3p CYCS 0.0778767956494004 0.272224943247293 0.024719343035275 0.675835191929842 miRNAWalker2_validate -0.105610171429319 0.000386651303177803 NA NA NA hsa-miR-19b-3p ENDOD1 -0.0138427450847924 0.856506310673067 0.00876481122735484 0.901919122541558 mirMAP -0.173543118088962 2.6650139209397e-05 NA NA NA hsa-miR-20a-3p ENDOD1 0.102743778777997 0.19677865359751 0.00876481122735484 0.901919122541558 MirTarget -0.130481894864718 0.000214730822004643 NA NA NA hsa-miR-361-5p EXOG 0.0107519600643302 0.882230614095565 0.00757802268144525 0.845557683813036 miRanda -0.127773403089084 1.29763629812448e-07 NA NA NA hsa-miR-7-1-3p EXOG -0.0894475316162393 0.37322359785108 0.00757802268144525 0.845557683813036 MirTarget -0.102782827790204 2.1483091619081e-13 NA NA NA hsa-miR-129-5p FADD -0.567133990117126 0.00116152215679936 0.216653418892308 1.55270131762646e-06 miRanda -0.101992367892993 3.27715082112241e-26 NA NA NA hsa-miR-504-5p FAS -0.510726779625117 6.12122269907368e-05 0.265125211236005 0.00536978445115343 miRNAWalker2_validate -0.123587493507518 0.000186057016947978 NA NA NA hsa-miR-139-5p IKBKB -0.647754756897633 2.15378307237163e-06 0.113293458902771 0.0150457519384782 miRanda -0.130032749382716 1.65206176469125e-33 NA NA NA hsa-miR-491-5p IKBKB -0.749881113074983 8.22859263179194e-09 0.113293458902771 0.0150457519384782 miRanda -0.106383336993955 5.8305133961577e-22 NA NA NA hsa-miR-105-5p IL1RAP -0.567459149851677 8.20858944634795e-06 0.717312765597358 4.10950689256162e-10 miRNATAP -0.103956449202651 0.00998575133794231 NA NA NA hsa-miR-129-5p IL1RAP -0.567133990117126 0.00116152215679936 0.717312765597358 4.10950689256162e-10 miRanda -0.107803226648902 0.000247483915875916 NA NA NA hsa-miR-135a-3p IL1RAP -0.525746284167595 6.65334050664395e-05 0.717312765597358 4.10950689256162e-10 MirTarget -0.189401246051 9.18708343382372e-07 NA NA NA hsa-miR-139-5p IL1RAP -0.647754756897633 2.15378307237163e-06 0.717312765597358 4.10950689256162e-10 miRanda -0.149542112615001 7.84715185528142e-05 NA NA NA hsa-miR-301a-3p IL1RAP 0.00956875714542704 0.916380325220259 0.717312765597358 4.10950689256162e-10 MirTarget -0.20460444436783 0.000406878458759045 NA NA NA hsa-miR-3065-5p IL1RAP -0.262608140481507 0.0414570974148034 0.717312765597358 4.10950689256162e-10 mirMAP -0.219531662859107 1.6313129283077e-08 NA NA NA hsa-miR-577 IL1RAP -0.382068371859275 0.0145513192729119 0.717312765597358 4.10950689256162e-10 mirMAP -0.117550665362917 0.000293151760641273 NA NA NA hsa-miR-132-3p IRAK1 -0.178379362833856 0.13335200877129 0.149223508163169 0.00671810855376067 miRNAWalker2_validate -0.101334790951572 1.83500362627184e-11 NA NA NA hsa-miR-328-3p IRAK1 -0.27021372890849 0.00243992234645524 0.149223508163169 0.00671810855376067 miRNAWalker2_validate -0.129430919692527 1.19784910766324e-09 NA NA NA hsa-miR-346 IRAK1 -0.577786425963268 2.10883270131593e-09 0.149223508163169 0.00671810855376067 miRanda -0.108390514963778 5.28953068263154e-10 NA NA NA hsa-miR-137 MAP3K14 -0.924849266740909 1.546832202049e-06 0.212699891188413 0.000374055421229529 MirTarget;PITA;miRanda;miRNATAP -0.111472449092906 7.99736039539788e-21 NA NA NA hsa-miR-346 MAP3K14 -0.577786425963268 2.10883270131593e-09 0.212699891188413 0.000374055421229529 miRanda -0.145514173051604 2.83732159243253e-09 NA NA NA hsa-miR-874-3p MAP3K14 -0.342846516198707 0.00227406462925841 0.212699891188413 0.000374055421229529 MirTarget -0.161463169618818 5.58488354179691e-14 NA NA NA hsa-miR-138-5p MYD88 -0.616087649920851 1.35200027885729e-05 0.413794389705469 3.45628586505903e-06 MirTarget -0.14950820343919 1.68371091611091e-08 NA NA NA hsa-miR-491-5p MYD88 -0.749881113074983 8.22859263179194e-09 0.413794389705469 3.45628586505903e-06 miRanda -0.149015631188272 1.31985549334199e-07 NA NA NA hsa-miR-1179 NFKB1 -0.117056451908751 0.310989525634765 0.146182859193676 0.0048570839713069 miRNATAP -0.140273206429428 5.35154124955416e-17 NA NA NA hsa-miR-508-3p NFKB1 -0.292045725850845 0.0158994578296519 0.146182859193676 0.0048570839713069 MirTarget;PITA;miRNATAP -0.101247627472892 1.16240035097528e-10 NA NA NA hsa-miR-9-5p NFKB1 -0.125377971246422 0.269896477802284 0.146182859193676 0.0048570839713069 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.336428914988368 4.98439106747222e-16 NA NA NA hsa-miR-335-5p NFKBIA 0.0938511632756978 0.336889056387694 -0.201443799611495 0.00824233274360403 miRNAWalker2_validate -0.13477450107991 7.56661183168011e-06 NA NA NA hsa-let-7c-5p NGF -0.04295851886768 0.676052842748691 -0.416457950464278 0.0030667540551424 MirTarget -0.360257350329555 1.22298479321338e-05 NA NA NA hsa-let-7e-5p NGF 0.0471189209570344 0.657255455444655 -0.416457950464278 0.0030667540551424 MirTarget -0.207654601525943 0.00237596292059546 NA NA NA hsa-let-7i-5p NGF 0.0952853587923244 0.192120688957249 -0.416457950464278 0.0030667540551424 MirTarget -0.412016019685442 0.000120893499183623 NA NA NA hsa-miR-217 NGF 0.540279882451941 0.00312206944061206 -0.416457950464278 0.0030667540551424 miRanda -0.106355636018351 0.00151830983295066 NA NA NA hsa-miR-320a NGF 0.092135599636725 0.243028758989929 -0.416457950464278 0.0030667540551424 miRanda -0.445535637234912 4.53615837617544e-06 NA NA NA hsa-miR-320b NGF 0.283477746304177 0.0005953763020907 -0.416457950464278 0.0030667540551424 miRanda -0.295144471999818 6.92199710987292e-05 NA NA NA hsa-miR-320c NGF 0.343259124189966 0.000248338401291051 -0.416457950464278 0.0030667540551424 miRanda -0.420643347773402 2.54365030935421e-10 NA NA NA hsa-miR-423-5p NGF 0.0965587900813789 0.148039180704338 -0.416457950464278 0.0030667540551424 MirTarget;miRNATAP -0.377352274624578 0.000280283896732406 NA NA NA hsa-miR-137 NTRK1 -0.924849266740909 1.546832202049e-06 0.207348057458216 0.0386791072527031 miRanda -0.117432442139169 1.38166281095279e-07 NA NA NA hsa-miR-146b-5p PIK3CA 0.441078059667297 0.000237619929889385 0.037489443318349 0.579330715365297 mirMAP -0.131612247931155 2.37773318343185e-08 NA NA NA hsa-miR-339-5p PIK3CA 0.273253751377152 0.0185300170790638 0.037489443318349 0.579330715365297 miRanda -0.104034425659594 8.90794530378182e-06 NA NA NA hsa-miR-361-5p PIK3CA 0.0107519600643302 0.882230614095565 0.037489443318349 0.579330715365297 mirMAP -0.180837597518142 0.000106487510577531 NA NA NA hsa-miR-501-5p PIK3CA 0.0878951859817452 0.309663377222443 0.037489443318349 0.579330715365297 mirMAP -0.121849868853655 0.000118457183740553 NA NA NA hsa-miR-130b-3p PIK3CB 0.503911467685709 9.46590588950823e-08 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.103286697323809 1.47078743054601e-08 NA NA NA hsa-miR-19a-3p PIK3CB 0.0466707843731911 0.576818938277755 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.106776286308973 5.5364329421429e-07 NA NA NA hsa-miR-19b-3p PIK3CB -0.0138427450847924 0.856506310673067 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.144620281397534 1.13083007810642e-08 NA NA NA hsa-miR-25-3p PIK3CB 0.316649850160985 0.000777982468387578 -0.219948641615068 6.65962821954743e-06 miRNATAP -0.107740273525281 5.28619562870356e-06 NA NA NA hsa-miR-126-3p PIK3CG 0.283844173647452 0.0045313860754322 0.426342297358177 0.000910429107358105 miRTarBase -0.253640716789511 9.06991114742445e-05 NA NA NA hsa-miR-137 PIK3CG -0.924849266740909 1.546832202049e-06 0.426342297358177 0.000910429107358105 miRanda -0.150716294097788 1.51412490486832e-07 NA NA NA hsa-miR-139-5p PIK3CG -0.647754756897633 2.15378307237163e-06 0.426342297358177 0.000910429107358105 miRanda -0.231442980598987 3.92120693342636e-08 NA NA NA hsa-miR-103a-3p PIK3R1 -0.147443193527362 0.128400914654149 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.281302889120328 4.63842851180526e-09 NA NA NA hsa-miR-106a-5p PIK3R1 0.110074064773897 0.13045815990621 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.141577511066847 0.000568718663709082 NA NA NA hsa-miR-106b-5p PIK3R1 0.167096183547615 0.0497544396383451 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.114620357579885 0.00229922204074265 NA NA NA hsa-miR-1301-3p PIK3R1 -0.105695714210758 0.152249184194132 -0.259275224308753 0.00106935451973482 MirTarget -0.230097168026832 5.13675721710063e-09 NA NA NA hsa-miR-150-5p PIK3R1 0.0645271721519611 0.513295658828718 -0.259275224308753 0.00106935451973482 MirTarget -0.105207403299517 0.000393418533820088 NA NA NA hsa-miR-15a-5p PIK3R1 0.142658985373611 0.0536219185009004 -0.259275224308753 0.00106935451973482 MirTarget -0.230258286136212 5.21596791619245e-07 NA NA NA hsa-miR-15b-5p PIK3R1 0.592863156692837 1.41971517354055e-10 -0.259275224308753 0.00106935451973482 MirTarget -0.235643490684548 5.7801231863435e-14 NA NA NA hsa-miR-16-2-3p PIK3R1 0.468816222075619 7.04377763166292e-05 -0.259275224308753 0.00106935451973482 MirTarget -0.139286207343494 2.58317956279833e-09 NA NA NA hsa-miR-16-5p PIK3R1 0.246024668671282 0.00346042804503716 -0.259275224308753 0.00106935451973482 MirTarget -0.121551450392141 0.00217223263389569 NA NA NA hsa-miR-185-5p PIK3R1 -0.0767432095218297 0.404113540276816 -0.259275224308753 0.00106935451973482 miRNATAP -0.168972197052794 1.52142067802957e-07 NA NA NA hsa-miR-186-5p PIK3R1 0.0606381894130505 0.514217717592464 -0.259275224308753 0.00106935451973482 mirMAP -0.273384834286173 4.58184035796298e-17 NA NA NA hsa-miR-199a-3p PIK3R1 0.41226331871076 0.000497136154474528 -0.259275224308753 0.00106935451973482 miRNATAP -0.150441413618647 2.58272678316472e-10 NA NA NA hsa-miR-199a-5p PIK3R1 0.174889610847968 0.0900027418912454 -0.259275224308753 0.00106935451973482 miRanda -0.169409376623758 6.00323276983558e-10 NA NA NA hsa-miR-199b-3p PIK3R1 0.411848361704393 0.000517815011892379 -0.259275224308753 0.00106935451973482 miRNATAP -0.149610269102807 2.9670988718931e-10 NA NA NA hsa-miR-199b-5p PIK3R1 0.436298593972862 0.00200225454383871 -0.259275224308753 0.00106935451973482 miRanda -0.158988773915679 1.96615064731078e-16 NA NA NA hsa-miR-200b-3p PIK3R1 0.245768526504511 0.0580744465744737 -0.259275224308753 0.00106935451973482 mirMAP -0.203398688722103 1.6080278648636e-21 NA NA NA hsa-miR-200c-3p PIK3R1 0.413752622631695 8.6936333715621e-06 -0.259275224308753 0.00106935451973482 mirMAP -0.313372851003899 1.11853298374691e-27 NA NA NA hsa-miR-21-5p PIK3R1 1.0504925175311 2.58081295984106e-11 -0.259275224308753 0.00106935451973482 miRNAWalker2_validate;MirTarget;miRNATAP -0.192193122796698 2.35557430088274e-23 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-22-5p PIK3R1 0.0437233965539527 0.639996108995995 -0.259275224308753 0.00106935451973482 mirMAP -0.26421716056902 5.35024336821731e-19 NA NA NA hsa-miR-221-3p PIK3R1 -0.0109507285071775 0.936440693394519 -0.259275224308753 0.00106935451973482 MirTarget -0.125242954911868 1.02528886125279e-09 NA NA NA hsa-miR-222-3p PIK3R1 0.107117065575197 0.423818119725697 -0.259275224308753 0.00106935451973482 MirTarget -0.132930686168698 1.29543187744781e-10 NA NA NA hsa-miR-28-5p PIK3R1 0.104003572105781 0.153594705579534 -0.259275224308753 0.00106935451973482 miRanda -0.263804174247158 1.47407900801522e-10 NA NA NA hsa-miR-29b-3p PIK3R1 -0.178522712044497 0.0856106432165465 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.145678229090259 1.47483484512088e-06 NA NA NA hsa-miR-320a PIK3R1 0.092135599636725 0.243028758989929 -0.259275224308753 0.00106935451973482 miRNATAP -0.135112165926423 0.00256497204146449 NA NA NA hsa-miR-320c PIK3R1 0.343259124189966 0.000248338401291051 -0.259275224308753 0.00106935451973482 miRNATAP -0.100797336995439 0.0011155444118643 NA NA NA hsa-miR-361-5p PIK3R1 0.0107519600643302 0.882230614095565 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.28102979614435 4.07903189307061e-09 NA NA NA hsa-miR-374a-3p PIK3R1 0.176226170437625 0.0343958848425446 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.321250741910251 7.52372972851822e-15 NA NA NA hsa-miR-424-5p PIK3R1 0.25140374049676 0.00709934939774983 -0.259275224308753 0.00106935451973482 MirTarget -0.179589895147171 9.52256678975884e-09 NA NA NA hsa-miR-455-3p PIK3R1 0.512701816402104 0.000459755983280155 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRNATAP -0.161697245880861 9.23450362337655e-18 NA NA NA hsa-miR-532-3p PIK3R1 0.150624483952741 0.0599635132177191 -0.259275224308753 0.00106935451973482 MirTarget -0.152797062588971 2.75979429090484e-05 NA NA NA hsa-miR-542-3p PIK3R1 0.0835995677900829 0.403838744494071 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRanda -0.126538995109648 4.83333922747737e-05 NA NA NA hsa-miR-548b-3p PIK3R1 0.340474544210108 0.00224491592019745 -0.259275224308753 0.00106935451973482 mirMAP -0.215757322792441 3.83990685247808e-18 NA NA NA hsa-miR-589-3p PIK3R1 0.00739371736361305 0.927768051649329 -0.259275224308753 0.00106935451973482 mirMAP -0.128475712773886 0.000234342022369328 NA NA NA hsa-miR-590-3p PIK3R1 0.312951515438396 0.000480455423843996 -0.259275224308753 0.00106935451973482 miRanda;mirMAP -0.281948259643294 1.55243456524174e-20 NA NA NA hsa-miR-590-5p PIK3R1 0.16946027035949 0.0386004382081678 -0.259275224308753 0.00106935451973482 MirTarget;PITA;miRanda;miRNATAP -0.237039982006307 4.44595475697952e-12 NA NA NA hsa-miR-93-5p PIK3R1 0.139766237971122 0.101057320076629 -0.259275224308753 0.00106935451973482 MirTarget;miRNATAP -0.261542441732551 2.52352152477154e-07 NA NA NA hsa-miR-125a-3p PIK3R2 0.183834985432376 0.106230647107917 0.0588428389717066 0.330993461191286 miRanda -0.129596879826368 7.29774749644301e-14 NA NA NA hsa-miR-135a-5p PIK3R2 -0.122734612943241 0.196985964641886 0.0588428389717066 0.330993461191286 MirTarget;miRNATAP -0.131986778854444 6.81341317924018e-10 NA NA NA hsa-miR-28-5p PIK3R2 0.104003572105781 0.153594705579534 0.0588428389717066 0.330993461191286 miRanda -0.220439409614541 1.97006000122873e-14 NA NA NA hsa-miR-30a-5p PIK3R2 -0.062942936611238 0.513851954700252 0.0588428389717066 0.330993461191286 miRNATAP -0.422270293356803 9.55851299393926e-41 NA NA NA hsa-miR-30b-5p PIK3R2 -0.0858101016322106 0.263243418521854 0.0588428389717066 0.330993461191286 miRNATAP -0.124149959343624 7.09161751121272e-05 NA NA NA hsa-miR-30c-5p PIK3R2 -0.0469114469943097 0.565934557464627 0.0588428389717066 0.330993461191286 miRNAWalker2_validate;miRNATAP -0.240340425585456 4.99963373312426e-15 NA NA NA hsa-miR-30d-5p PIK3R2 -0.0607010868720046 0.471410043548794 0.0588428389717066 0.330993461191286 miRNATAP -0.157898559984916 9.76384983421278e-06 NA NA NA hsa-miR-30e-5p PIK3R2 0.0764521828640934 0.41904991629622 0.0588428389717066 0.330993461191286 miRNATAP -0.28288573453624 2.98060149038092e-23 NA NA NA hsa-miR-331-3p PIK3R2 0.0126984726684189 0.905175053956974 0.0588428389717066 0.330993461191286 MirTarget -0.109375813751675 5.11486213404741e-09 NA NA NA hsa-miR-339-5p PIK3R2 0.273253751377152 0.0185300170790638 0.0588428389717066 0.330993461191286 miRanda -0.141580109769359 1.93632849649246e-17 NA NA NA hsa-miR-34a-5p PIK3R2 0.143472919835524 0.211283228567057 0.0588428389717066 0.330993461191286 MirTarget -0.127777793508925 2.24958566486742e-13 NA NA NA hsa-miR-9-3p PIK3R3 -0.184945845984695 0.0290051908009009 0.266207970572364 5.77735138484935e-05 mirMAP -0.147414732691119 0.000303043365501486 NA NA NA hsa-miR-9-5p PIK3R3 -0.125377971246422 0.269896477802284 0.266207970572364 5.77735138484935e-05 miRNATAP -0.234645741849474 2.452186835562e-05 NA NA NA hsa-miR-106b-5p PPP3CA 0.167096183547615 0.0497544396383451 -0.373235846566351 7.56900657293056e-08 miRNATAP -0.156975600850481 8.08375589469546e-06 NA NA NA hsa-miR-17-5p PPP3CA 0.0975751369167082 0.20621110214601 -0.373235846566351 7.56900657293056e-08 miRNATAP -0.156864057675735 0.000234156174021412 NA NA NA hsa-miR-20a-5p PPP3CA -0.0115865752288853 0.887069448776018 -0.373235846566351 7.56900657293056e-08 miRNATAP -0.11299242225162 0.0039148602825986 NA NA NA hsa-miR-21-5p PPP3CA 1.0504925175311 2.58081295984106e-11 -0.373235846566351 7.56900657293056e-08 miRNATAP -0.130424810796755 2.20070954535807e-12 NA NA NA hsa-miR-320a PPP3CA 0.092135599636725 0.243028758989929 -0.373235846566351 7.56900657293056e-08 miRanda -0.211827782030443 4.01920568101511e-07 NA NA NA hsa-miR-320b PPP3CA 0.283477746304177 0.0005953763020907 -0.373235846566351 7.56900657293056e-08 miRanda -0.144192030306541 6.21193412642773e-06 NA NA NA hsa-miR-320c PPP3CA 0.343259124189966 0.000248338401291051 -0.373235846566351 7.56900657293056e-08 miRanda -0.159502579873982 3.05354814536541e-08 NA NA NA hsa-miR-450b-5p PPP3CA 0.270544879013992 0.00801537838499562 -0.373235846566351 7.56900657293056e-08 PITA;mirMAP;miRNATAP -0.158857807775905 1.0971728867913e-09 NA NA NA hsa-miR-93-3p PPP3CA 0.0778767956494004 0.272224943247293 -0.373235846566351 7.56900657293056e-08 miRNATAP -0.103322549875116 0.00846397616128606 NA NA NA hsa-miR-93-5p PPP3CA 0.139766237971122 0.101057320076629 -0.373235846566351 7.56900657293056e-08 miRNATAP -0.294073353489622 5.63338473261847e-10 NA NA NA hsa-miR-15a-5p PPP3CB 0.142658985373611 0.0536219185009004 -0.433160664971454 7.64786199353402e-10 miRNATAP -0.154879727290926 0.000565344774724231 NA NA NA hsa-miR-15b-5p PPP3CB 0.592863156692837 1.41971517354055e-10 -0.433160664971454 7.64786199353402e-10 miRNATAP -0.237305735014458 6.56456884638479e-15 NA NA NA hsa-miR-30a-5p PPP3CB -0.062942936611238 0.513851954700252 -0.433160664971454 7.64786199353402e-10 miRNATAP -0.216347543291675 3.88335269674995e-06 NA NA NA hsa-miR-320a PPP3CB 0.092135599636725 0.243028758989929 -0.433160664971454 7.64786199353402e-10 miRanda -0.283610796821456 3.73775473194107e-11 NA NA NA hsa-miR-320b PPP3CB 0.283477746304177 0.0005953763020907 -0.433160664971454 7.64786199353402e-10 miRanda -0.214071216687752 5.61901615376464e-11 NA NA NA hsa-miR-320c PPP3CB 0.343259124189966 0.000248338401291051 -0.433160664971454 7.64786199353402e-10 miRanda -0.241503669878515 1.61057546698162e-16 NA NA NA hsa-miR-339-5p PPP3CB 0.273253751377152 0.0185300170790638 -0.433160664971454 7.64786199353402e-10 miRanda -0.14695495240965 2.98975197156432e-10 NA NA NA hsa-miR-424-5p PPP3CB 0.25140374049676 0.00709934939774983 -0.433160664971454 7.64786199353402e-10 miRNATAP -0.174803267163998 9.35958492728842e-09 NA NA NA hsa-miR-450b-5p PPP3CB 0.270544879013992 0.00801537838499562 -0.433160664971454 7.64786199353402e-10 MirTarget;PITA;miRNATAP -0.145409462742278 7.85383237188849e-08 NA NA NA hsa-miR-452-5p PPP3CB 0.599327068454211 1.02031931068531e-05 -0.433160664971454 7.64786199353402e-10 MirTarget;miRNATAP -0.100917826632377 3.76376326737129e-07 NA NA NA hsa-miR-501-5p PPP3CB 0.0878951859817452 0.309663377222443 -0.433160664971454 7.64786199353402e-10 PITA;miRNATAP -0.110974250509852 0.000497821238894801 NA NA NA hsa-miR-590-3p PPP3CB 0.312951515438396 0.000480455423843996 -0.433160664971454 7.64786199353402e-10 miRanda -0.13889306492083 5.08331239857392e-06 NA NA NA hsa-miR-500a-3p PPP3CC 0.0479457078972052 0.49420307623914 -0.0407919825674279 0.323390220477665 MirTarget;miRNATAP -0.107532400267747 7.33877743778064e-06 NA NA NA hsa-miR-27a-3p PPP3R1 0.303760032895792 0.00457375391189806 -0.284481378057962 3.41822765696265e-05 miRNATAP -0.102375961650782 9.77727749562009e-05 NA NA NA hsa-miR-320a PPP3R1 0.092135599636725 0.243028758989929 -0.284481378057962 3.41822765696265e-05 PITA;miRanda -0.228132769092911 2.62987783565586e-08 NA NA NA hsa-miR-320b PPP3R1 0.283477746304177 0.0005953763020907 -0.284481378057962 3.41822765696265e-05 PITA;miRanda -0.139121452927635 9.32245529079262e-06 NA NA NA hsa-miR-320c PPP3R1 0.343259124189966 0.000248338401291051 -0.284481378057962 3.41822765696265e-05 PITA;miRanda -0.155051607654631 4.4227147992575e-08 NA NA NA hsa-miR-93-5p PPP3R1 0.139766237971122 0.101057320076629 -0.284481378057962 3.41822765696265e-05 MirTarget;miRNATAP -0.159907992777141 0.000711555619167879 NA NA NA hsa-miR-195-3p PRKACB 0.100105455903079 0.133362389112338 -0.220744787859509 0.000769904555544923 mirMAP -0.108467815706043 0.00216235374196591 NA NA NA hsa-miR-19b-3p PRKACB -0.0138427450847924 0.856506310673067 -0.220744787859509 0.000769904555544923 miRNAWalker2_validate;MirTarget;miRNATAP -0.139345436156152 4.31724651745926e-05 NA NA NA hsa-miR-93-5p PRKACB 0.139766237971122 0.101057320076629 -0.220744787859509 0.000769904555544923 miRNAWalker2_validate -0.173086797632099 2.75308372550666e-05 NA NA NA hsa-miR-92b-3p PRKAR1B -0.213968504161963 0.0523921855715716 -0.331226636103815 0.00185179476729062 miRNATAP -0.265817698930862 1.38898557302028e-08 NA NA NA hsa-miR-106a-5p PRKAR2A 0.110074064773897 0.13045815990621 0.0499067218930502 0.570803320010815 mirMAP -0.141138122291596 0.00949689935152969 NA NA NA hsa-let-7a-3p PRKAR2B 0.0530563276543763 0.370749834463566 -0.24734867831596 0.0054348511673324 mirMAP -0.228149226557569 0.00169780711744157 NA NA NA hsa-miR-186-5p PRKAR2B 0.0606381894130505 0.514217717592464 -0.24734867831596 0.0054348511673324 mirMAP -0.267274767580659 3.05255004468226e-09 NA NA NA hsa-miR-200c-3p PRKAR2B 0.413752622631695 8.6936333715621e-06 -0.24734867831596 0.0054348511673324 miRNATAP -0.13325047530009 0.00121280207587678 NA NA NA hsa-miR-3065-5p PRKAR2B -0.262608140481507 0.0414570974148034 -0.24734867831596 0.0054348511673324 miRNATAP -0.309749621250972 1.31097841951162e-27 NA NA NA hsa-miR-452-5p PRKAR2B 0.599327068454211 1.02031931068531e-05 -0.24734867831596 0.0054348511673324 miRNATAP -0.122443645327783 1.13414328084408e-05 NA NA NA hsa-miR-590-3p PRKAR2B 0.312951515438396 0.000480455423843996 -0.24734867831596 0.0054348511673324 miRNAWalker2_validate;miRanda -0.153660965394327 0.000328905831720416 NA NA NA hsa-miR-9-5p PRKAR2B -0.125377971246422 0.269896477802284 -0.24734867831596 0.0054348511673324 miRNATAP -0.258128532651296 0.00222017338400696 NA NA NA hsa-miR-92b-3p PRKAR2B -0.213968504161963 0.0523921855715716 -0.24734867831596 0.0054348511673324 miRNATAP -0.294147751494198 1.32984295508085e-13 NA NA NA hsa-miR-103a-2-5p PRKX -0.0677071239234235 0.496202425310213 -0.234656702391627 0.00431000884646852 mirMAP -0.185614107823291 3.00149445409194e-08 NA NA NA hsa-miR-126-5p PRKX 0.177332403948664 0.0711675116243123 -0.234656702391627 0.00431000884646852 MirTarget;miRNATAP -0.187301575968566 1.30428005782524e-07 NA NA NA hsa-miR-139-5p PRKX -0.647754756897633 2.15378307237163e-06 -0.234656702391627 0.00431000884646852 MirTarget -0.111049071581413 1.00400340749505e-05 NA NA NA hsa-miR-140-3p PRKX -0.123864258867705 0.14796686619449 -0.234656702391627 0.00431000884646852 mirMAP -0.188792670005692 0.000271546600802212 NA NA NA hsa-miR-143-5p PRKX 0.460068066898162 0.000603073392139956 -0.234656702391627 0.00431000884646852 miRNATAP -0.143226065795783 5.12157339907867e-09 NA NA NA hsa-miR-145-5p PRKX 0.108948399943015 0.310032352776693 -0.234656702391627 0.00431000884646852 MirTarget;miRNATAP -0.222996299939014 7.11591241346387e-12 NA NA NA hsa-miR-148a-3p PRKX 0.306821592493439 0.0392356958885949 -0.234656702391627 0.00431000884646852 MirTarget -0.107273294595938 5.70709078555767e-06 NA NA NA hsa-miR-148b-3p PRKX 0.0710353380371513 0.322794068460232 -0.234656702391627 0.00431000884646852 MirTarget -0.256323857849908 1.74249911988869e-06 NA NA NA hsa-miR-148b-5p PRKX 0.127111784487801 0.188731110216917 -0.234656702391627 0.00431000884646852 mirMAP -0.119235002449208 0.000703942471696724 NA NA NA hsa-miR-16-1-3p PRKX 0.346674816118499 1.86447850167636e-05 -0.234656702391627 0.00431000884646852 MirTarget -0.185074407382809 8.05451956693482e-06 NA NA NA hsa-miR-16-2-3p PRKX 0.468816222075619 7.04377763166292e-05 -0.234656702391627 0.00431000884646852 MirTarget;mirMAP -0.198629542094723 6.08018799340144e-13 NA NA NA hsa-miR-181a-5p PRKX -0.178215604431845 0.0700010802255601 -0.234656702391627 0.00431000884646852 mirMAP -0.15106825040288 0.00123868308466424 NA NA NA hsa-miR-181b-5p PRKX 0.0260899284211646 0.768941836912518 -0.234656702391627 0.00431000884646852 mirMAP -0.279522155835593 2.18438847332363e-09 NA NA NA hsa-miR-19a-3p PRKX 0.0466707843731911 0.576818938277755 -0.234656702391627 0.00431000884646852 miRNATAP -0.124806422804607 0.00303499773107959 NA NA NA hsa-miR-19b-3p PRKX -0.0138427450847924 0.856506310673067 -0.234656702391627 0.00431000884646852 miRNATAP -0.176601983702423 0.000423189660787107 NA NA NA hsa-miR-218-5p PRKX -0.534287404275829 0.00308322441944796 -0.234656702391627 0.00431000884646852 miRNATAP -0.149237768038395 7.9885738260038e-17 NA NA NA hsa-miR-23b-3p PRKX 0.0476610245860094 0.630277683372344 -0.234656702391627 0.00431000884646852 mirMAP -0.19980057428488 1.12685512772789e-07 NA NA NA hsa-miR-26a-2-3p PRKX 0.26597501648608 0.000796444054340143 -0.234656702391627 0.00431000884646852 mirMAP -0.134802899007735 0.00144817261413182 NA NA NA hsa-miR-27b-3p PRKX -0.0599187745243146 0.58208143475992 -0.234656702391627 0.00431000884646852 MirTarget;miRNATAP -0.131368529715051 0.000112253562391766 NA NA NA hsa-miR-29a-3p PRKX -0.224140851576083 0.0349516103550236 -0.234656702391627 0.00431000884646852 mirMAP -0.123863087459517 0.00269106286247111 NA NA NA hsa-miR-29b-3p PRKX -0.178522712044497 0.0856106432165465 -0.234656702391627 0.00431000884646852 mirMAP -0.173413136803338 1.43367881926089e-06 NA NA NA hsa-miR-29c-3p PRKX -0.372180390387259 0.00202634431973208 -0.234656702391627 0.00431000884646852 mirMAP -0.117968228195243 0.000111258392242527 NA NA NA hsa-miR-323b-3p PRKX -0.254474308066903 0.15651329633719 -0.234656702391627 0.00431000884646852 mirMAP -0.121471311882467 3.96651880271252e-11 NA NA NA hsa-miR-335-3p PRKX 0.34540584335595 0.00043848660222294 -0.234656702391627 0.00431000884646852 mirMAP -0.251127596351802 4.37732142750105e-13 NA NA NA hsa-miR-33a-5p PRKX 0.0197996465877179 0.86830656038963 -0.234656702391627 0.00431000884646852 mirMAP -0.10391806415594 0.000224291426001427 NA NA NA hsa-miR-375 PRKX -0.0268401141324031 0.858358101444697 -0.234656702391627 0.00431000884646852 miRNAWalker2_validate;miRanda -0.115694809758222 1.47169050286958e-07 NA NA NA hsa-miR-409-3p PRKX 0.00456692516634361 0.974143243758506 -0.234656702391627 0.00431000884646852 mirMAP -0.224291050887867 1.11264823229881e-22 NA NA NA hsa-miR-432-5p PRKX -0.214242438141977 0.166186087759946 -0.234656702391627 0.00431000884646852 miRNATAP -0.167538839875961 1.88486920036252e-15 NA NA NA hsa-miR-450b-5p PRKX 0.270544879013992 0.00801537838499562 -0.234656702391627 0.00431000884646852 mirMAP -0.102960665807675 0.00204956194458089 NA NA NA hsa-miR-495-3p PRKX -0.421982588962504 0.00504424038526901 -0.234656702391627 0.00431000884646852 MirTarget -0.178449482518289 2.15988126863328e-16 NA NA NA hsa-miR-539-5p PRKX -0.326485413953598 0.0554453076411246 -0.234656702391627 0.00431000884646852 mirMAP -0.144010574364934 5.81971773063992e-14 NA NA NA hsa-miR-542-3p PRKX 0.0835995677900829 0.403838744494071 -0.234656702391627 0.00431000884646852 mirMAP -0.154230342664725 3.08460179424332e-05 NA NA NA hsa-miR-590-3p PRKX 0.312951515438396 0.000480455423843996 -0.234656702391627 0.00431000884646852 mirMAP -0.149486589624489 6.13061408540306e-05 NA NA NA hsa-miR-625-3p PRKX -0.0638840950852737 0.547354471747105 -0.234656702391627 0.00431000884646852 mirMAP -0.214773811263468 1.78365907489191e-11 NA NA NA hsa-miR-935 PRKX -0.277848050075718 0.02166231531395 -0.234656702391627 0.00431000884646852 mirMAP -0.134302509100497 1.78644955549975e-06 NA NA NA hsa-miR-1224-5p RELA -0.53941068839383 0.00201058335769201 0.0739196932169914 0.126314845681798 mirMAP -0.105338562253176 5.76511309726817e-46 NA NA NA hsa-miR-138-5p RELA -0.616087649920851 1.35200027885729e-05 0.0739196932169914 0.126314845681798 miRNATAP -0.118615517312994 4.30114332551203e-37 NA NA NA hsa-miR-324-5p RELA 0.0183370482495953 0.842996782677271 0.0739196932169914 0.126314845681798 miRNAWalker2_validate;miRanda -0.154645221576587 4.11812534839311e-25 NA NA NA hsa-miR-330-5p RELA -0.262932095995605 0.0465226565966658 0.0739196932169914 0.126314845681798 miRanda -0.120880678711014 2.77817174522426e-33 NA NA NA hsa-miR-488-3p RELA -0.257955761012568 0.00280795162806256 0.0739196932169914 0.126314845681798 mirMAP -0.149905441066056 1.6489641903294e-20 NA NA NA hsa-miR-769-5p RELA -0.320572895263071 0.00425481410304398 0.0739196932169914 0.126314845681798 miRNATAP -0.185846508366017 6.56909022979932e-59 NA NA NA hsa-miR-940 RELA -0.0183807673806575 0.881747775878136 0.0739196932169914 0.126314845681798 mirMAP -0.130602708311796 2.82198893322046e-34 NA NA NA hsa-miR-488-3p RIPK1 -0.257955761012568 0.00280795162806256 0.245886626810937 4.80588359675208e-08 mirMAP -0.105841910087552 4.06227509659865e-08 NA NA NA hsa-miR-26b-5p TNFRSF10B -0.0984536538762768 0.231803769574623 0.334610949770233 2.80844341840702e-05 mirMAP -0.145112479707621 0.00300053851841411 NA NA NA hsa-miR-3065-5p TNFRSF10B -0.262608140481507 0.0414570974148034 0.334610949770233 2.80844341840702e-05 mirMAP -0.219007549297091 5.79943007315713e-18 NA NA NA hsa-miR-30c-1-3p TNFRSF10B 0.0175008295781193 0.849381871022214 0.334610949770233 2.80844341840702e-05 MirTarget -0.162621201182926 1.0567643617516e-05 NA NA NA hsa-miR-331-3p TNFRSF10B 0.0126984726684189 0.905175053956974 0.334610949770233 2.80844341840702e-05 miRNAWalker2_validate -0.139320916932824 1.1678251691796e-05 NA NA NA hsa-miR-3622a-3p TNFRSF10B 0.0389203848593471 0.75605812962459 0.334610949770233 2.80844341840702e-05 mirMAP -0.109923831583013 5.54121970241808e-05 NA NA NA hsa-miR-940 TNFRSF10B -0.0183807673806575 0.881747775878136 0.334610949770233 2.80844341840702e-05 miRNAWalker2_validate -0.152290945518939 1.36170749375405e-08 NA NA NA hsa-miR-491-5p TNFRSF10C -0.749881113074983 8.22859263179194e-09 0.563588988846446 3.79455543373386e-08 miRanda -0.133647368900062 9.07292337479097e-05 NA NA NA hsa-miR-139-5p TNFRSF10D -0.647754756897633 2.15378307237163e-06 0.31666092938901 0.000315848582429834 miRanda -0.11935610561564 3.25321994093021e-05 NA NA NA hsa-miR-139-5p TNFSF10 -0.647754756897633 2.15378307237163e-06 0.394738086337697 7.46036026828867e-05 miRanda -0.111786211059875 0.000654822266934151 NA NA NA hsa-miR-125a-5p TP53 -0.0833349424517387 0.325440200253607 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase;miRanda -0.375445863722298 1.2184854773952e-16 26389681;21777146;23079745 Mechanistically inactivation of miR-125a during cervical carcinogenesis was caused by HPV suppression of p53 expression;In addition wild-type p53 mRNA and protein expression was increased by hsa-miR-125a-5p overexpression; Moreover blocking wild-type p53 attenuated the effect of hsa-miR-125a-5p on apoptosis; In loss-of-function experiments wild-type p53 mRNA and protein expression was decreased by blocking hsa-miR-125a-5p; The effect of hsa-miR-125a-5p inhibitor on apoptosis was also weakened by blocking wild-type p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity tumorigenesis;; cervical and endocervical cancer;lung cancer;liver cancer hsa-miR-125b-5p TP53 -0.0119698382910496 0.890233923197358 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase -0.294628443669371 1.07324994036762e-07 24169356;24846940;26335100;24137477;26686386;24402874;21399871;23585871;24762088;23079745 MiR 125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK independent pathways; Further studies reveal that p53 is regulated by miR-125b; However downregulation of the endogenous miR-125b also results in p53-independent apoptotic pathway leading to apoptosis in p53 mutated U251 cells and p53 knockdown U87 cells;The results revealed that hsa-miR-125b may regulate multiple biological processes and signal transduction pathways and drug-resistant occurrence is associated with cell proliferation cell apoptosis cell cycle and signaling pathways including MAPK Wnt and p53;The carboxy terminal domain of connexin 43 CT Cx43 modulates the expression of p53 by altering miR 125b expression in low grade human breast cancers; In addition CT-Cx43 was exogenously expressed in the breast cancer-derived cell line MCF-7 and its effect on the expression of miR-125b and its downstream target p53 were evaluated as well as its effect on cellular proliferation and death using MTT and LDH assays respectively; Interestingly we found that miR-125b a negative regulator of p53 exhibited an inverse expression relationship with CT-Cx43 in the breast cancer samples tested;TP53 and hsa-miR-125b were observed to form a self-adaptation association;In this study we have investigated the delivery and transfection of wild-type wt- p53 and microRNA-125b miR-125b expressing plasmid DNA in SK-LU-1 human lung adenocarcinoma cells as well as in KrasG12D/p53fl/fl KP genetically engineered mouse model of lung cancer;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;In vitro assays revealed that overexpression of miR-125b repressed the endogenous level of p53 protein in human colorectal cancer cells;In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b;A stable overexpression of miR-125b in human melanoma cell line Mel-Juso resulted in a G0/G1 cell cycle block and emergence of large cells expressing senescence markers: senescence-associated beta-galactosidase p21 p27 and p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity; To show the clinical significance of these findings mutations in the DNA binding domain of p53 and promoter methylation of miR-125b were investigated; Four out of nine patients with induced SIRT7 carried mutations in the p53 gene and one patient showed hypermethylation of the miR-125b promoter region ;;;;;immune resistance;;;; glioblastoma;gastric cancer;breast cancer;pancreatic cancer;lung cancer;cervical and endocervical cancer;colorectal cancer;prostate cancer;melanoma;liver cancer hsa-miR-150-5p TP53 0.0645271721519611 0.513295658828718 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.156281214913021 3.09404029899862e-06 23747308;23228962 miR 150 promotes the proliferation of lung cancer cells by targeting P53; Here we demonstrate that miR-150 is aberrantly upregulated in lung cancer tissue and negatively correlates with the expression of the proapoptotic gene p53 but not EGR2; We show that miR-150 specifically targets the 3'-UTR of p53 and regulates its expression; Inhibition of miR-150 effectively delays cell proliferation and promotes apoptosis accompanied by increased p53 protein expression;miR-150 and miR-3940-5p were found to be significantly downregulated in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA; miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect pathway ; lung cancer;lung squamous cell cancer hsa-miR-221-3p TP53 -0.0109507285071775 0.936440693394519 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.143405645468124 6.89189438710031e-10 20880178;24324033 Circulating miR 221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression; The correlation between miR-221 levels and protein levels of p53 CEA ER and PR clinicopathological features or overall survival was analyzed; The immunohistochemistry analysis demonstrates a significant correlation between plasma miR-221 level and p53 expression; The direct amplification of plasma miR-221 can be used as a potential noninvasive molecular marker for diagnosis and prognosis of CRC and is correlated with p53 expression;Interestingly miR-221 can activate the p53/mdm2 axis by inhibiting MDM2 and in turn p53 activation contributes to miR-221 enhanced expression; Moreover by modulating the p53 axis miR-221 impacts cell-cycle progression and apoptotic response to doxorubicin in hepatocellular carcinoma-derived cell lines; These data were confirmed in clinical specimens of hepatocellular carcinoma in which elevated miR-221 expression was associated with the simultaneous presence of wild-type p53 and DNA hypomethylation poor survival;worse prognosis;progression;drug resistance colorectal cancer;liver cancer hsa-miR-222-3p TP53 0.107117065575197 0.423818119725697 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.142653482163308 1.2671214916531e-09 NA NA NA hsa-miR-30d-5p TP53 -0.0607010868720046 0.471410043548794 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.336903694979927 3.32137008004591e-09 NA NA NA hsa-miR-324-5p TP53 0.0183370482495953 0.842996782677271 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.170645382602635 1.21583558752455e-06 NA NA NA hsa-miR-338-3p TP53 -0.39963704679384 0.0034410643220691 0.346312114104146 1.42081328137217e-05 miRanda -0.117560999445537 4.2049029410775e-06 NA NA NA hsa-miR-34a-5p TP53 0.143472919835524 0.211283228567057 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.127236433475415 7.74576992902642e-06 23155233;19773441;25572695;25123132;22438124;21702042;22292433;21383543;25982144;19443717;26790955;24642471;22198213;25362853;23036084;19421141;24444609;20186752;22457788;27186405;21731696;19736307;26879132;23569431;25771001;23292869;24209638;24957404;21240262;24503183;22102859;18803879;24630988;25895459;25894979;19714243;26944831;19921694;21909380;25789847;23450486;24402874;24337371;18497571;25490093;22593438;24528540;20145172;23349340;25038915;21321636;23632240;23624843;19029026;20309940;22810507;23862748;25932212;18834855;21225432 The high miR-34a expression level in the cells after irradiation at 60 Gy reduced the p53 expression level;MicroRNA-34a miR-34a is a transcriptional target of p53 that is down-regulated in some cancer cell lines; Analysis of human specimens showed that miR-34a expression is down-regulated in glioblastoma tissues as compared with normal brain and in mutant p53 gliomas as compared with wild-type p53 gliomas;For this purpose cell viability assay Realtime quantitative PCR for mRNA quantification western blot for essential protein expression p53 silencing by shRNA and miR-34a knockdown were performed in the present study; PRE also elevated p53 protein and triggered miR-34a expression;As expected p53 loss caused downregulation of established p53 targets e.g p21 and miR-34 family and increased proliferation in both luminal and basal-like cell lines;Moreover re-expression of miR-34a by transfection in NSCLC cells resulted in inhibition of cell growth and invasiveness induction of apoptosis and enhanced p53 activity;MiR 34a chemosensitizes bladder cancer cells to cisplatin treatment regardless of p53 Rb pathway status; MiR-34a is a downstream effector of p53 that has been shown to target several molecules associated with cell cycle and cell survival pathways; As alterations in these pathways are frequent in muscle invasive transitional cell carcinoma of the bladder MI-TCC for example mutation or loss of p53 and Rb the goal of this study was to determine whether manipulation of miR-34a expression levels could abrogate the effect of these alterations and sensitize bladder cancer cells to chemotherapy;Reporter assay further showed that NF-kappaB-induced miR-34a transcriptional activity was reduced by p53 impairment; And wildtype p53 is responsible for NF-kappaB-mediated miR-34a transcriptional activity but not for NF-kappaB binding;Recently miR-34 family has been shown to be part of the p53 pathway which is frequently involved in lung cancer and the expression of miR-34 has been reported to be regulated by DNA methylation;5 Aminolevulinic acid mediated sonodynamic therapy induces anti tumor effects in malignant melanoma via p53 miR 34a Sirt1 axis; Therefore the p53 miR-34a and SIRT1 constituted a positive feedback loop;miR-34a expression could be increased in Y79 cells but not Weri-Rb1 cells after p53 activation; This differential regulation was not caused by genomic alterations at the miR-34a p53 binding site or mature gene;Conversely kallistatin stimulated expression of the tumorigenic suppressors miR-34a and p53;Members of the miR-34 family are induced by the tumor suppressor p53 and are known to inhibit epithelial-to-mesenchymal transition EMT and therefore presumably suppress the early phases of metastasis;MicroRNA-34a miR-34a a transcriptional target of p53 is a well-known tumor suppressor gene; Moreover we found that miR-34a was downregulated in 25 of 40 62.5% colon cancer tissues as compared with the adjacent normal colon tissues and that the expression of miR-34a was correlated with the DNA-binding activity of p53;Ectopic expression of miR-34a-5p in p53 wild-type colon cancer cell HCT116 significantly inhibited cell growth migration invasion and metastasis; miR-34a-5p induced cell apoptosis cell cycle arrest at G1 phase and p53 transcription activity in HCT116 cells but not in the HCT116 p53 knockout p53-/- cells; miR-34a-5p significantly suppressed the HCT116 growth in vivo whereas it showed no effect on the HCT116 p53-/- xenograft indicating that the growth-inhibiting effect by miR-34a-5p was dependent on p53;microRNA 34a sensitizes lung cancer cell lines to DDP treatment independent of p53 status; However the precise biological role of miR-34a in p53 deficient lung cancer cell lines remains largely elusive; Overall in this study we found the proliferation inhibition function of miR-34a in vitro in lung cancer cell lines is p53 independent and also demonstrated the combination therapeutic potential of miR-34a and DDP in lung cancer cell lines;In particular mammalian miR-34 is upregulated by p53 in response to radiation but little is known about the role of this miRNA in vivo; These findings show a role for mir-34 in both apoptotic and non-apoptotic cell death in vivo much like that of cep-1 the C elegans p53 homolog;Tumor-suppressive miR-34a a direct target of p53 has been shown to target several molecules of cell survival pathways; Here we show that capsaicin-induced oxidative DNA damage culminates in p53 activation to up-regulate expression of miR-34a in non-small cell lung carcinoma NSCLC cells;Accumulating evidence suggests that miR-34a as a key mediator of p53 tumor suppression is aberrantly expressed in human cancers; Bioinformatics analysis produced a protein-protein interaction network which revealed that the p53 signaling pathway and cell cycle pathway were two major hubs containing most of the proteins regulated by miR-34a;Studies have demonstrated that miR-34a which is a direct target of the p53 tumor suppressor gene functions as a tumor suppressor and is associated with the tumor growth and metastasis of various human malignances;Knockdown of oncoprotein E6 expression of human papillomavirus in SiHa and HeLa cells by siRNAs lead to an increased protein level of p53 decreased level of miR-34a as well as reduced Warburg effect;miR-34a is known as a p53 regulated tumor suppressor microRNA in many cancer types;In addition in patients with sufficient tumor tissue we assessed p53 mutations and the methylation status of the MIRN34A gene promoter region and correlated these findings with miR-34a expression; Patients with both p53 mutations and low miR-34a levels had the highest probability of relapse P = 0.001;Particularly miR-34a has been revealed to be a direct transcriptional target of p53 which is frequently mutated in epithelial ovarian carcinomas especially in high grade serous cancer; The aim of this study was to investigate the clinical relevance of mir34a as well as its promoter methylation in a subset of 133 ovarian cancers with a special focus on the p53 mutation status the dualistic type I and type II ovarian cancer model and the different histotypes; The expression of miR-34a was found lower in type II than in type I cancers p = 0.037 in p53 mutated as compared to p53 wild type cancers p = 0.003 and in high grade compared to in low grade cancers p = 0.028; The inverse association between miR-34a expression and grading p53 mutation status and dualistic tumor type classification together with its prognostic relevance may underline the tumor-suppressive character of miR-34a in ovarian cancer;As a direct target gene of p53 miR-34a has been suggested to mediate the tumor suppressor function of p53;Further studies indicated that PEG4000-TQ-Nps could significantly increase the expression of miR-34a through p53;Downregulation of miR 34a in breast tumors is not associated with either p53 mutations or promoter hypermethylation while it correlates with metastasis; Recent studies have shown that p53 upregulates miR-34 family leading to direct repression of several key oncogenes; In this study expression of mature miR-34a in breast tumors with wild-type p53 was investigated in order to find any correlation between dysregulation of miR-34a expression and breast cancer; In about 40 % of the wild-type p53 samples miR-34a was significantly downregulated; This study has provided evidence that miR-34a expression can be affected in a significant proportion of breast tumors independent of p53; Knowledge of miR-34a status may provide additional useful information regarding the nature of breast tumors especially when p53 testing does not show any aberration;Among these we found well studied molecules such as the miR-17-92 cluster comprising potent oncogenic microRNA and miR-34 recently found to interact with p53;The results demonstrated that miR-199a and miR-34a could induce the apoptosis of human osteosarcoma cells via p53 signalling pathway;Here we show through expression analysis that miR-34a a p53 target was underexpressed in CD44+ prostate cancer cells purified from xenograft and primary tumors;Members of the miR-34 family have been shown to be transcriptional targets of the tumour suppressor gene P53;Functional genomic analysis highlighted a novel regulatory role of the transcription factor MAZ apart from the known control by p53 on the expression of miR-34a and a number of miR-34a targets; Our analysis for regulatory loops suggest that MAZ and p53 transcription factors co-operate in modulating miR-34a as well as miR-34a targets involved in several cellular pathways;Restoration of tumor suppressor miR 34 inhibits human p53 mutant gastric cancer tumorspheres; MicroRNA miR-34 was recently found to be a direct target of p53 functioning downstream of the p53 pathway as a tumor suppressor; Our results demonstrate that in p53-deficient human gastric cancer cells restoration of functional miR-34 inhibits cell growth and induces chemosensitization and apoptosis indicating that miR-34 may restore p53 function;miR 34 cooperates with p53 in suppression of prostate cancer by joint regulation of stem cell compartment; The miR-34 family was originally found to be a direct target of p53 and is a group of putative tumor suppressors; Here we report that mice with prostate epithelium-specific inactivation of mir-34 and p53 show expansion of the prostate stem cell compartment and develop early invasive adenocarcinomas and high-grade prostatic intraepithelial neoplasia whereas no such lesions are observed after inactivation of either the mir-34 or p53 genes alone by 15 months of age; Consistently combined deficiency of p53 and miR-34 leads to acceleration of MET-dependent growth self-renewal and motility of prostate stem/progenitor cells; Our study provides direct genetic evidence that mir-34 genes are bona fide tumor suppressors and identifies joint control of MET expression by p53 and miR-34 as a key component of prostate stem cell compartment regulation aberrations in which may lead to cancer;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however the effect of miR-34a on the ovarian cancer cells remains unclear;The microRNA-34 family miR-34a -34b and -34c have been reported to be tumor suppressor microRNAs miRNAs that are regulated by the TP53 and DNA hypermethylation; To elucidate the roles of miR-34 family in colon carcinogenesis miR-34a/b/c were measured in tumors and adjacent noncancerous tissues from 159 American and 113 Chinese colon cancer patients using quantitative RT-PCR and we examined associations between miR-34a/b/c expression with TNM staging cancer-specific mortality TP53 mutation status and Affymetrix microarray data;Transcription of the three miRNA miR-34 family members was recently found to be directly regulated by p53; Our results demonstrate that miR-34 may restore at least in part the tumor suppressing function of the p53 in p53-deficient human pancreatic cancer cells;p53 status was not correlated with miR-34a;The above results suggest that microRNA-34a is one of the important components of PRIMA-1-induced apoptotic network in the cancer cells harboring mutant p53;MicroRNA-34a miR-34a is a transcriptional target of p53 and is down-regulated in pancreatic cancer;Phytochemical regulation of the tumor suppressive microRNA miR 34a by p53 dependent and independent responses in human breast cancer cells; The tumor suppressive microRNA miR-34a is transcriptionally regulated by p53 and shown to inhibit breast cancer cell proliferation as well as being a marker of increased disease free survival; Human breast cancer cells expressing wild-type MCF-7 or mutant p53 T47D were treated with a concentration range and time course of each phytochemical under conditions of cell cycle arrest as detected by flow cytometry to examine the potential connection between miR-34a expression and their anti-proliferative responses; Our results suggest that miR-34a is an essential component of the anti-proliferative activities of I3C artemisinin and artesunate and demonstrate that both wild-type p53 dependent and independent pathways are responsible for miR-34a induction;Compared with p53 normal group the expression level of miR-34a was significantly lower in p53 deletion group;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;The microRNA miR-34 family is a direct transcriptional target of tumor-suppressor TP53 and loss of miR-34 function may impair TP53-mediated cell cycle arrest and apoptosis;It appears that AR-dependent inhibition of p53 resulted in suppression of miR-34a and -34c expression;In this study we evaluated the cascade of events determined by etoposide-induced DNA damage in OS cell lines with different p53 status focusing on methylation status and expression of miR-34a that modulate tumor cell growth and cell cycle progression; Our results suggest that the open and unmethylated conformation of the miR-34a gene may be regulated by p53 able to bind the gene promoter;The miR-34 family under-expressed in-non small cell lung cancers NSCLCs are effectors of p53 activation upon irradiation of cells;MiR-34a a direct p53 target gene possesses tumor-suppressive properties as they mediate apoptosis cell cycle arrest and senescence;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in human epithelial ovarian cancer EOC; miR-34a expression is decreased in 100% and miR-34b*/c in 72% of EOC with p53 mutation whereas miR-34a is also downregulated in 93% of tumors with wild-type p53; Finally miR-34 reconstitution experiments in p53 mutant EOC cells resulted in reduced proliferation motility and invasion the latter of which was dependent on MET expression;miR-34a is transcriptionally induced by the tumor suppressor gene p53 which is often downregulated in non-small cell lung cancer NSCLC;In conclusion overexpression of PEBP4 reduced the sensitivity of A549 cells to DDP-induced cytotoxicity mainly through the altered expression of the p53 protein or the modulation of miR-34a;To investigate the effects of miR-449 and miR-34 on cell growth cell cycle and target gene expression based on these miRNA different expressions in ovarian cancer cell lines SKOV3 and SKOV3-ipl both with mutation of p53;Numerous studies have focused on the association between miR-34 family members which are direct p53 targets and carcinogenesis of many cancers including hepatocellular carcinoma HCC;p53 regulates nuclear GSK 3 levels through miR 34 mediated Axin2 suppression in colorectal cancer cells;MicroRNA-34a miR-34a a potential key effector of the p53 tumor-suppressor gene was studied as a potential tumor suppressor in uveal melanoma;The miR-34 family members are direct transcriptional targets of tumor suppressor p53 and loss of miR-34 function can impair p53-mediated cell cycle arrest and apoptosis;Profiling microRNA miRNA expression delineated TP53 alteration-associated miRNA profiles and identified miR-34a and miR-100 as the most significantly down- and upregulated miRNA respectively; Clinically low miR-34a expression and TP53 alterations predicted for chemotherapy resistance and inferior outcome; Thus detailed molecular profiling links impaired p53 to decreased miR-34a expression but also identifies p53-independent miR-34a induction mechanisms as shown in TP53biallelic altered cell lines treated with 15-deoxy-Δ1214-prostaglandin;MiR-34a a direct target of p53 has been shown to target several molecules associated with the cell cycle and cell survival pathways and its dysregulation is implicated in cancer drug resistance or sensitivity in several human cancers;MicroRNA-34a miR-34a is a direct transcriptional target of p53 and links tumor suppressor function and the oncogenic pathways in some cancers;Tumor suppressor p53 transcriptionally regulates expression of microRNA-34a which confers translational inhibition and mRNA degradation of genes involved in cell cycle control and apoptosis; MiR-34a expression was markedly reduced in p53-null PC3 cells and p53-mutated DU145 cells compared with LNCaP cells expressing wild-type p53;The microRNA encoding genes miR-34a and miR-34b/c represent direct p53 target genes and possess tumor suppressive properties as they mediate apoptosis cell cycle arrest and senescence; In the colorectal cancer samples a statistically significant correlation of miR-34a methylation and the absence of p53 mutation was detected; The mutual exclusiveness of miR-34a methylation and p53 mutation indicates that miR-34a inactivation may substitute for loss of p53 function in cancer ;;;;;poor survival;;;malignant trasformation;;;metastasis;;metastasis;;drug resistance;poor survival;;metastasis;;;;;;;metastasis;;;;;;;motility;;staging;tumorigenesis;;;;;drug resistance;poor survival;;immune resistance;;;progression;;;motility;;;;tumorigenesis;;;;drug resistance;poor survival;drug resistance;;; glioblastoma;glioblastoma;bladder cancer;breast cancer;lung squamous cell cancer;bladder cancer;esophageal cancer;lung squamous cell cancer;melanoma;retinoblastoma;breast cancer;colorectal cancer;colon cancer;colorectal cancer;lung cancer;breast cancer;lung squamous cell cancer;liver cancer;sarcoma;cervical and endocervical cancer;lung squamous cell cancer;lung squamous cell cancer;ovarian cancer;sarcoma;breast cancer;breast cancer;head and neck cancer;sarcoma;prostate cancer;kidney renal cell cancer;breast cancer;gastric cancer;prostate cancer;ovarian cancer;colon cancer;pancreatic cancer;esophageal cancer;lung cancer;pancreatic cancer;breast cancer;B cell lymphoma;cervical and endocervical cancer;colorectal cancer;prostate cancer;sarcoma;lung squamous cell cancer;esophageal cancer;ovarian cancer;lung squamous cell cancer;lung cancer;ovarian cancer;liver cancer;colorectal cancer;melanoma;liver cancer;acute myeloid leukemia;liver cancer;gastric cancer;prostate cancer;sarcoma hsa-miR-380-5p TP53 -0.356387125389103 0.0163515117264043 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.130032370502874 2.53621800466177e-09 NA NA NA hsa-miR-381-3p TP53 -0.192118086401438 0.203198554750071 0.346312114104146 1.42081328137217e-05 MirTarget -0.10958470195975 2.36383687034549e-07 NA NA NA hsa-miR-421 TP53 -0.171573235029879 0.0518112933651029 0.346312114104146 1.42081328137217e-05 miRanda -0.12756767272718 0.000455424352559313 NA NA NA hsa-miR-491-5p TP53 -0.749881113074983 8.22859263179194e-09 0.346312114104146 1.42081328137217e-05 MirTarget;miRanda -0.210461133541589 1.59624493209635e-19 23519249 Targeted site prediction indicated that both Bcl-XL and TP53 contain miR-491-5p recognizing sites in their 3' UTRs; Overexpression of miR-491-5p in the pancreatic cancer cell line SW1990 effectively inhibited both endogenous Bcl-XL and TP53 gene expressions pancreatic cancer hsa-miR-504-5p TP53 -0.510726779625117 6.12122269907368e-05 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;MirTarget -0.175599590135581 1.78517568542536e-12 25015107 TFF1 activates p53 through down regulation of miR 504 in gastric cancer; Alternatively we found that the reconstitution of TFF1 down-regulates miR-504 a negative regulator of p53; Western blot analysis data demonstrated that miR-504 abrogates TFF1-induced p53 protein expression and activity; In conclusion the in vitro and in vivo data demonstrate for the first time a novel mechanism by which the tumor suppressor functions of TFF1 involve activation of p53 through down-regulation of miR-504 gastric cancer hsa-miR-28-3p XIAP 0.139444423971018 0.0811187936164314 -0.0661021704479872 0.136970038691812 mirMAP;miRNATAP -0.13703937596377 1.19630131083778e-13 NA NA NA hsa-miR-374a-3p XIAP 0.176226170437625 0.0343958848425446 -0.0661021704479872 0.136970038691812 mirMAP -0.10515524409523 1.00927208583495e-09 NA NA NA hsa-miR-708-3p XIAP 0.00458267260363954 0.962455132950708 -0.0661021704479872 0.136970038691812 mirMAP -0.104572018460759 9.50687666565819e-16 NA NA NA