miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-125b-5p AKT1 -1.94393269763542 2.78180543229264e-58 0.675644771423915 4.31393881321574e-23 miRNAWalker2_validate;miRTarBase -0.198341990959858 1.58124486218174e-28 NA NA NA hsa-miR-143-3p AKT1 -1.23248181631499 4.74160976678504e-28 0.675644771423915 4.31393881321574e-23 miRNAWalker2_validate -0.113351907486334 3.74647210660112e-08 NA NA NA hsa-miR-24-3p AKT2 -0.120123317289941 0.21432376743243 -0.0243409112523851 0.696831042278932 mirMAP -0.108581040434418 8.76204903485834e-07 NA NA NA hsa-miR-30e-3p AKT2 -0.708952170420973 1.05866470714978e-22 -0.0243409112523851 0.696831042278932 mirMAP -0.10769217628588 0.000202699653075448 NA NA NA hsa-miR-106b-5p AKT3 1.22477789913175 2.31787213143326e-32 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.328917679851073 3.2761525214021e-13 NA NA NA hsa-miR-107 AKT3 0.678998026350662 2.56224148930538e-16 -1.39153139819913 1.56022353185962e-23 PITA;miRanda -0.322045444014362 3.05085750718847e-08 NA NA NA hsa-miR-15a-5p AKT3 0.812445324157528 4.50263417099822e-20 -1.39153139819913 1.56022353185962e-23 miRNAWalker2_validate;miRTarBase;miRNATAP -0.50537113863267 1.94212195956378e-21 NA NA NA hsa-miR-15b-5p AKT3 0.788659356634696 1.13678211092534e-11 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.193152993862959 3.52162993117826e-06 NA NA NA hsa-miR-16-5p AKT3 0.46835991343556 1.91125356547776e-06 -1.39153139819913 1.56022353185962e-23 miRNAWalker2_validate;miRTarBase;miRNATAP -0.137142128931012 0.00593844300257035 NA NA NA hsa-miR-181a-5p AKT3 0.635863607630183 6.72486918157744e-11 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.151700435123375 0.00241569058552761 NA NA NA hsa-miR-181b-5p AKT3 1.22749257795094 1.21998198830919e-27 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.228362092376509 5.2632904676588e-08 NA NA NA hsa-miR-20a-5p AKT3 0.44979911120222 0.000602386964327228 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.142287905559561 0.000137185408492637 NA NA NA hsa-miR-29b-2-5p AKT3 -0.263499971180298 0.0400001755611965 -1.39153139819913 1.56022353185962e-23 mirMAP -0.174192327083419 4.9828909918003e-06 NA NA NA hsa-miR-29b-3p AKT3 0.438847962603729 6.46722433236121e-05 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.240095261108435 6.25012390753699e-08 26512921 MicroRNA 29B mir 29b regulates the Warburg effect in ovarian cancer by targeting AKT2 and AKT3 ovarian cancer hsa-miR-29c-3p AKT3 -0.0652732378039769 0.563774115756922 -1.39153139819913 1.56022353185962e-23 miRNAWalker2_validate;miRNATAP -0.188130633733952 1.47230271027192e-05 NA NA NA hsa-miR-340-5p AKT3 1.0857159627596 4.94471062546756e-24 -1.39153139819913 1.56022353185962e-23 mirMAP -0.283834227072162 1.26866636163553e-10 NA NA NA hsa-miR-769-5p AKT3 0.564871766265874 8.16695101656941e-10 -1.39153139819913 1.56022353185962e-23 PITA;miRNATAP -0.188201328225218 0.000387633821142586 NA NA NA hsa-miR-93-5p AKT3 1.01788600209314 1.24312921618126e-21 -1.39153139819913 1.56022353185962e-23 miRNATAP -0.358774978428685 5.45504344144073e-16 NA NA NA hsa-miR-429 ANGPT1 2.84108892259147 3.88520896426515e-64 -3.16300498545581 8.56501022163231e-73 miRanda -0.493163485450717 1.16635414095955e-46 NA NA NA hsa-miR-590-3p ANGPT1 1.27438512890514 1.03164723481469e-23 -3.16300498545581 8.56501022163231e-73 PITA;miRanda;mirMAP -0.249579211964837 2.08892020158711e-06 NA NA NA hsa-miR-148a-5p ANGPT2 0.572692271754695 2.14306251522796e-06 -0.549211576203072 3.52083224037155e-07 mirMAP -0.103375715857729 0.000741562565982671 NA NA NA hsa-miR-19a-3p ANGPT2 0.817507952171848 3.76285090469625e-08 -0.549211576203072 3.52083224037155e-07 MirTarget -0.131690138905339 9.69188420190022e-08 NA NA NA hsa-miR-19b-3p ANGPT2 0.139124391010031 0.255658553872934 -0.549211576203072 3.52083224037155e-07 MirTarget -0.155260376407316 3.00137101828932e-07 NA NA NA hsa-miR-429 ANGPT2 2.84108892259147 3.88520896426515e-64 -0.549211576203072 3.52083224037155e-07 miRanda -0.156050420625197 1.27561726808804e-14 NA NA NA hsa-miR-664a-3p ANGPT2 -0.237699857923843 0.0197071390183375 -0.549211576203072 3.52083224037155e-07 mirMAP -0.10026929390526 0.00617721939067824 NA NA NA hsa-miR-140-3p ATF2 -1.25844395244141 5.29222651039892e-33 -0.526197310314373 1.91997984112617e-07 MirTarget;PITA;miRNATAP -0.166236312082199 1.656661080244e-07 NA NA NA hsa-miR-15a-5p ATF2 0.812445324157528 4.50263417099822e-20 -0.526197310314373 1.91997984112617e-07 miRNAWalker2_validate -0.357209405082962 3.17144140577998e-21 NA NA NA hsa-miR-181a-5p ATF2 0.635863607630183 6.72486918157744e-11 -0.526197310314373 1.91997984112617e-07 mirMAP;miRNATAP -0.275655700176318 3.25056114494222e-15 NA NA NA hsa-miR-181b-5p ATF2 1.22749257795094 1.21998198830919e-27 -0.526197310314373 1.91997984112617e-07 mirMAP;miRNATAP -0.242609990251896 1.87882820169588e-16 NA NA NA hsa-miR-181c-5p ATF2 0.574847839298605 4.51287502066478e-07 -0.526197310314373 1.91997984112617e-07 mirMAP;miRNATAP -0.163465374342035 6.83965645814107e-08 NA NA NA hsa-miR-19a-3p ATF2 0.817507952171848 3.76285090469625e-08 -0.526197310314373 1.91997984112617e-07 MirTarget;miRNATAP -0.104880819897631 6.18713149818057e-06 NA NA NA hsa-miR-21-5p ATF2 2.32007501846389 3.08394781855554e-137 -0.526197310314373 1.91997984112617e-07 miRNAWalker2_validate -0.231001727277097 1.50432513865861e-13 NA NA NA hsa-miR-221-3p ATF2 -0.317970198201501 0.00910855267413448 -0.526197310314373 1.91997984112617e-07 MirTarget -0.164933797555005 6.40232509155629e-09 NA NA NA hsa-miR-26a-5p ATF2 -0.481180084102235 1.82368246174758e-10 -0.526197310314373 1.91997984112617e-07 MirTarget;miRNATAP -0.136339162056602 0.00311093260377358 NA NA NA hsa-miR-361-5p ATF2 -0.0996867547900457 0.095428250126326 -0.526197310314373 1.91997984112617e-07 miRanda -0.473542579976889 4.72282680855827e-16 NA NA NA hsa-miR-374a-5p ATF2 -0.239709888246804 0.00309819667153627 -0.526197310314373 1.91997984112617e-07 mirMAP -0.142980511322363 0.000948593180528854 NA NA NA hsa-miR-127-3p BAD -1.02337931491694 2.9749745518131e-15 0.671335929306102 6.05368129718844e-13 miRanda;miRNATAP -0.12231920555334 5.77371456889348e-07 NA NA NA hsa-miR-326 BAD -1.54449030848141 1.04703417845594e-17 0.671335929306102 6.05368129718844e-13 miRanda -0.162992963378128 2.73003852781454e-21 NA NA NA hsa-miR-130b-5p BCL2 1.0573161199584 2.55177349228739e-12 -0.895845361347303 1.04475176246219e-07 mirMAP -0.298072526149562 1.58526199405774e-15 27364335 The level of microRNA-130b in relationship with the expression of PPARγ VEGF-A BCL-2 and apoptosis were analyzed in 91 lung cancer patient samples using immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick end labeling TUNEL assay on tissue microarrays; In vitro and in vivo miR-130b enrichment associated with down-regulation of PPARγ up-regulation of VEGF-A and BCL-2 and decreased apoptosis lung cancer hsa-miR-136-5p BCL2 0.0557692528524285 0.703264372780941 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.134479560632769 0.000722378019814531 NA NA NA hsa-miR-142-3p BCL2 1.53951237846104 2.06769936282034e-17 -0.895845361347303 1.04475176246219e-07 miRanda -0.1459796770776 3.09176521823541e-06 NA NA NA hsa-miR-148a-3p BCL2 0.706969798912638 5.52538085480485e-09 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.220300574731754 3.43414567895446e-06 21455217;23975374 MiR 148a promotes apoptosis by targeting Bcl 2 in colorectal cancer;MiR 148a regulates the growth and apoptosis in pancreatic cancer by targeting CCKBR and Bcl 2; Using western blot and luciferase activity assay CCKBR and Bcl-2 were identified as targets of miR-148a; Moreover we also found that the expression of Bcl-2 lacking in 3'UTR could abrogate the pro-apoptosis function of miR-148a ; colorectal cancer;pancreatic cancer hsa-miR-15a-5p BCL2 0.812445324157528 4.50263417099822e-20 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.390139881240154 1.08000528360666e-09 25594541;22335947;26915294;18931683;25623762 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;The expression of MiR-15a was significantly inhibited by Bcl-2 P < 0.05;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MicroRNAs miRNAs are noncoding small RNAs that repress protein translation by targeting specific messenger RNAs miR-15a and miR-16-1 act as putative tumor suppressors by targeting the oncogene BCL2;miR 15a and miR 16 modulate drug resistance by targeting bcl 2 in human colon cancer cells; To investigate the reversal effect of targeted modulation of bcl-2 expression by miR-15a and miR-16 on drug resistance of human colon cancer cells ;;poor survival;;drug resistance breast cancer;breast cancer;lung cancer;prostate cancer;colon cancer hsa-miR-15b-3p BCL2 1.12898579025388 1.18050912917317e-14 -0.895845361347303 1.04475176246219e-07 mirMAP -0.310741788828047 6.78718604611496e-16 25594541;26915294;26884837;18449891 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MiR 15b mediates liver cancer cells proliferation through targeting BCL 2; MiR-15b overexpression downregulated BCL2 mRNA and protein expression obviously P < 0.05; On the contrary miR-15b inhibitor transfection markedly reduced miR-15b expression in liver cancer cells P < 0.05 promoted tumor cell proliferation and increased BCL2 mRNA and protein expression; MiR-15b can inhibit HepG2 cell proliferation and down-regulate BCL2 mRNA and protein expression;miR 15b and miR 16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells; The downregulation of miR-15b and miR-16 in SGC7901/VCR cells was concurrent with the upregulation of Bcl-2 protein; Taken together our findings suggest that miR-15b and miR-16 could play a role in the development of MDR in gastric cancer cells at least in part by modulation of apoptosis via targeting BCL2 ;poor survival;;drug resistance breast cancer;lung cancer;liver cancer;gastric cancer hsa-miR-15b-5p BCL2 0.788659356634696 1.13678211092534e-11 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.282455107593279 9.40085716451768e-09 25594541;26915294;26884837;18449891 MicroRNAs miRNAs encoded by the miR-15 cluster are known to induce G1 arrest and apoptosis by targeting G1 checkpoints and the anti-apoptotic B cell lymphoma 2 BCL-2 gene;As a result transcript levels of the tumor-suppressive miR-15 and let-7 families increased which targeted and decreased the expression of the crucial prosurvival genes BCL-2 and BCL-XL respectively;MiR 15b mediates liver cancer cells proliferation through targeting BCL 2; MiR-15b overexpression downregulated BCL2 mRNA and protein expression obviously P < 0.05; On the contrary miR-15b inhibitor transfection markedly reduced miR-15b expression in liver cancer cells P < 0.05 promoted tumor cell proliferation and increased BCL2 mRNA and protein expression; MiR-15b can inhibit HepG2 cell proliferation and down-regulate BCL2 mRNA and protein expression;miR 15b and miR 16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells; The downregulation of miR-15b and miR-16 in SGC7901/VCR cells was concurrent with the upregulation of Bcl-2 protein; Taken together our findings suggest that miR-15b and miR-16 could play a role in the development of MDR in gastric cancer cells at least in part by modulation of apoptosis via targeting BCL2 ;poor survival;;drug resistance breast cancer;lung cancer;liver cancer;gastric cancer hsa-miR-16-1-3p BCL2 1.30142784811196 2.53343713753744e-29 -0.895845361347303 1.04475176246219e-07 mirMAP -0.226601532507069 1.00488294259221e-05 18931683 MicroRNAs miRNAs are noncoding small RNAs that repress protein translation by targeting specific messenger RNAs miR-15a and miR-16-1 act as putative tumor suppressors by targeting the oncogene BCL2 prostate cancer hsa-miR-16-2-3p BCL2 0.286355254959005 0.0256946046884474 -0.895845361347303 1.04475176246219e-07 mirMAP -0.220617147018903 1.20839624748242e-06 NA NA NA hsa-miR-17-5p BCL2 0.599531757298529 1.07318660415906e-05 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.416922204785524 7.52642290968327e-24 25435430 Combined overexpression of miR-16 and miR-17 greatly reduced Beclin-1 and Bcl-2 expressions respectively; miR-17 overexpression reduced cytoprotective autophagy by targeting Beclin-1 whereas overexpression of miR-16 potentiated paclitaxel induced apoptotic cell death by inhibiting anti-apoptotic protein Bcl-2 lung cancer hsa-miR-181a-5p BCL2 0.635863607630183 6.72486918157744e-11 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.399174853804926 9.83051271483704e-12 24335172;24267199;23740615;25524579;27534652 The function role of miR 181a in chemosensitivity to adriamycin by targeting Bcl 2 in low invasive breast cancer cells; Luciferase reporter vector with the putative BCL-2 3' untranslated region 3'UTR was constructed to explore whether BCL-2 was a direct target gene of miR-181a; Real-time PCR was performed to test the expression of miR-181a and Bcl-2 in the selected breast cancer tissue samples; Transfected with miR-181a mimic in cells resulted in the decreased expression of Bcl-2; At least in part the detection of miR-181a may direct the clinical medication in patients with neoadjuvant chemotherapy because of miR-181a enhanced adriamycin-induced apoptosis via targeting Bcl-2;Bufalin was found to induce the expression of miR-181a a small non-coding RNA believed to induce apoptosis by repressing its target gene BCL-2; In prostate cancer PC-3cell line bufalin-induced apoptosis can be largely attenuated by a miR-181a inhibitor which blocked bufalin-induced Bcl-2 reduction and caspase-3 activation;The results from Western blotting indicated that miR-181a might be associated with the up-regulation of bcl-2 and matrix metalloproteinase 9 and the down-regulation of tissue inhibitor of metalloproteinases-3 and p21 in MG63 cells;Moreover we also found miR-181 reduction was associated with increased Bcl-2 levels and miR-181 was further suggested to exert its pro-apoptotic function mainly through targeting Bcl-2 expression;In addition inhibition of miR-181a promoted apoptosis of HeLa and CaSKi cells due to increasing Bax expression and decreasing Bcl-2 expression ;;;; breast cancer;prostate cancer;sarcoma;lung squamous cell cancer;cervical and endocervical cancer hsa-miR-181b-5p BCL2 1.22749257795094 1.21998198830919e-27 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.46875883187563 8.08303248718094e-22 25524579 Moreover we also found miR-181 reduction was associated with increased Bcl-2 levels and miR-181 was further suggested to exert its pro-apoptotic function mainly through targeting Bcl-2 expression lung squamous cell cancer hsa-miR-186-5p BCL2 -0.426987301460247 1.04129380549315e-05 -0.895845361347303 1.04475176246219e-07 mirMAP -0.348265462226792 5.09173882444383e-09 NA NA NA hsa-miR-192-5p BCL2 1.33366224451777 9.5116159697107e-29 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate -0.170899649695256 0.000266209546627792 26550150 MicroRNA 192 regulates chemo resistance of lung adenocarcinoma for gemcitabine and cisplatin combined therapy by targeting Bcl 2; In this paper we try to test whether miR-192 regulates chemo-resistance in human carcinoma A549 mice model by targeting Bcl-2; MTT assay real-time RT-PCR western blotting assay were used to investigate miR-192 expression levels cell viability ratio and Bcl-2 protein expression levels; Bcl-2 mRNA and protein expression levels up-regulated in miR-192 inhibitor treated tumor; Bcl-2 is a key regulator for miR-192 related chemotherapy resistance; In this study we demonstrate that miR-192 regulates chemoresistance for gemcitabine and cisplatin combined chemotherapy in human adenocarcinoma lung cancer A549 cells and Bcl-2 is the target of miR-192 drug resistance lung cancer hsa-miR-20a-3p BCL2 -0.218902047543766 0.179868363625547 -0.895845361347303 1.04475176246219e-07 mirMAP -0.13906123260096 9.78039595655978e-05 NA NA NA hsa-miR-20a-5p BCL2 0.44979911120222 0.000602386964327228 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.398529315160521 3.65662136244948e-20 27044389 We found that upregulated expression of miR-17 and miR-20a correlates with lack of pro-apoptotic Bim expression lymphoma hsa-miR-21-5p BCL2 2.32007501846389 3.08394781855554e-137 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.486116466611641 4.94993012898121e-21 26555418;21468550;25994220;25381586;23359184;22964582;21376256 The expression of Bax Bcl-2 and miR-21 in parental and paclitaxel-resistant cells was detected by RT-PCR and Western blotting;BCL-2 up-regulation could be achieved by miR-21 overexpression which prevented T24 cells from apoptosis induced by doxorubicin; Furthermore the miR-21 induced BCL-2 up-regulation could be cancelled by the PI3K inhibitor LY294002;Meanwhile miR-21 loss reduced STAT3 and Bcl-2 activation causing an increase in the apoptosis of tumour cells in CAC mice;Changes in the sensitivity of osteosarcoma cells to CDDP were examined after transfection with miR-21 mimics or anti-miR-21 or bcl-2 siRNA in combination with CDDP;Resveratrol induces apoptosis of pancreatic cancers cells by inhibiting miR 21 regulation of BCL 2 expression; We also used Western blot to measure BCL-2 protein levels after down-regulation of miR-21 expression; Besides down-regulation of miR-21 expression can inhibit BCL-2 expression in PANC-1 CFPAC-1 and MIA Paca-2 cells; Over-expression of miR-21 expression can reverse down-regulation of BCL-2 expression and apoptosis induced by resveratrol; In this study we demonstrated that the effect of resveratrol on apoptosis is due to inhibiting miR-21 regulation of BCL-2 expression;Tumors harvested from these lungs have elevated levels of oncogenic miRNAs miR-21 and miR-155; are deficient for p53-regulated miRNAs; and have heightened expression of miR-34 target genes such as Met and Bcl-2;Bcl 2 upregulation induced by miR 21 via a direct interaction is associated with apoptosis and chemoresistance in MIA PaCa 2 pancreatic cancer cells; However the roles and mechanisms of miRNA miR-21 in regulation of Bcl-2 in pancreatic cancer remain to be elucidated; Then luciferase activity was observed after miR-21 mimics and pRL-TK plasmids containing wild-type and mutant 3'UTRs of Bcl-2 mRNA were co-transfected; Cells transfected with miR-21 inhibitor revealed an opposite trend. There was a significant increase in luciferase activity in the cells transfected with the wild-type pRL-TK plasmid in contrast to those transfected with the mutant one indicating that miR-21 promotes Bcl-2 expression by binding directly to the 3'UTR of Bcl-2 mRNA; Upregulation of Bcl-2 directly induced by miR-21 is associated with apoptosis chemoresistance and proliferation of MIA PaCa-2 pancreatic cancer cells ;;;;;;drug resistance breast cancer;bladder cancer;colorectal cancer;sarcoma;pancreatic cancer;lung cancer;pancreatic cancer hsa-miR-224-5p BCL2 -2.22249420723892 1.07787949391113e-18 -0.895845361347303 1.04475176246219e-07 mirMAP -0.241222882395465 2.18051025428057e-28 24796455 In addition the expressions of Bcl2 mRNA and protein were 1.05 ± 0.04 and 0.21 ± 0.03 in the miR-224 ASO group significantly lower than that in the control group 4.87 ± 0.96 and 0.88 ± 0.09 P < 0.01 gastric cancer hsa-miR-24-2-5p BCL2 -0.222445793556803 0.059573849265115 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.314657429269267 1.22690370959234e-10 NA NA NA hsa-miR-29a-5p BCL2 0.334183773952055 0.00445137265870197 -0.895845361347303 1.04475176246219e-07 mirMAP -0.158728142208395 0.00138976145130829 20041405 Subsequent investigation characterized two antiapoptotic molecules Bcl-2 and Mcl-1 as direct targets of miR-29; Furthermore silencing of Bcl-2 and Mcl-1 phenocopied the proapoptotic effect of miR-29 whereas overexpression of these proteins attenuated the effect of miR-29 liver cancer hsa-miR-33a-5p BCL2 1.35185127289399 9.80509199125785e-12 -0.895845361347303 1.04475176246219e-07 mirMAP -0.201771252418707 4.45169669138265e-12 NA NA NA hsa-miR-34a-5p BCL2 0.129201805242403 0.133261654068224 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase -0.21690891602483 0.00143015924808101 24565525;22623155;21399894;23155233;24444609;20687223;24988056;18803879;19714243;25053345;20433755;22964582;23862748 In vitro and in vivo experiments showed that miR-34a and DOX can be efficiently encapsulated into HA-CS NPs and delivered into tumor cells or tumor tissues and enhance anti-tumor effects of DOX by suppressing the expression of non-pump resistance and anti-apoptosis proto-oncogene Bcl-2;MiR 34a inhibits proliferation and migration of breast cancer through down regulation of Bcl 2 and SIRT1; In this study we aimed to determine the effect of miR-34a on the growth of breast cancer and to investigate whether its effect is achieved by targeting Bcl-2 and SIRT1; Bcl-2 and SIRT1 as the targets of miR-34a were found to be in reverse correlation with ectopic expression of miR-34a;Quantitative PCR and western analysis confirmed decreased expression of two genes BCL-2 and CCND1 in docetaxel-resistant cells which are both targeted by miR-34a;The miR-34a expression levels in cells after irradiation at 30 and 60 Gy were 0.17- and 18.7-times the BCL2 and caspase-9 expression levels respectively;Functional analyses further indicate that restoration of miR-34a inhibits B cell lymphoma-2 Bcl-2 protein expression to withdraw the survival advantage of these resistant NSCLC cells;Thus in PC3PR cells reduced expression of miR-34a confers paclitaxel resistance via up-regulating SIRT1 and Bcl2 expression; MiR-34a and its downstream targets SIRT1 and Bcl2 play important roles in the development of paclitaxel resistance all of which can be useful biomarkers and promising therapeutic targets for the drug resistance in hormone-refractory prostate cancer;Target analysis indicated that micro RNA miR-34a directly regulates Bcl-2 and miR-34a overexpression decreased Bcl-2 protein level in gastric cancer cells; We also found that luteolin upregulates miR-34a expression and downregulates Bcl-2 expression; Based on these results we can draw the conclusion that luteolin partly decreases Bcl-2 expression through upregulating miR-34a expression;miR-34 targets Notch HMGA2 and Bcl-2 genes involved in the self-renewal and survival of cancer stem cells; Human gastric cancer cells were transfected with miR-34 mimics or infected with the lentiviral miR-34-MIF expression system and validated by miR-34 reporter assay using Bcl-2 3'UTR reporter; Human gastric cancer Kato III cells with miR-34 restoration reduced the expression of target genes Bcl-2 Notch and HMGA2; Bcl-2 3'UTR reporter assay showed that the transfected miR-34s were functional and confirmed that Bcl-2 is a direct target of miR-34; The mechanism of miR-34-mediated suppression of self-renewal appears to be related to the direct modulation of downstream targets Bcl-2 Notch and HMGA2 indicating that miR-34 may be involved in gastric cancer stem cell self-renewal/differentiation decision-making;Among the target proteins regulated by miR-34 are Notch pathway proteins and Bcl-2 suggesting the possibility of a role for miR-34 in the maintenance and survival of cancer stem cells; Our data support the view that miR-34 may be involved in pancreatic cancer stem cell self-renewal potentially via the direct modulation of downstream targets Bcl-2 and Notch implying that miR-34 may play an important role in pancreatic cancer stem cell self-renewal and/or cell fate determination;Manipulating miR-34a in prostate cancer cells confirms that this miRNA regulates BCL-2 and may in part regulate response to docetaxel;For instance miR-34a up-regulation corresponded with a down-regulation of BCL2 protein; Treating Par-4-overexpressing HT29 cells with a miR-34a antagomir functionally reversed both BCL2 down-regulation and apoptosis by 5-FU;Tumors harvested from these lungs have elevated levels of oncogenic miRNAs miR-21 and miR-155; are deficient for p53-regulated miRNAs; and have heightened expression of miR-34 target genes such as Met and Bcl-2;MicroRNA 34a targets Bcl 2 and sensitizes human hepatocellular carcinoma cells to sorafenib treatment; HCC tissues with lower miR-34a expression displayed higher expression of Bcl-2 protein than those with high expression of miR-34a; therefore an inverse correlation is evident between the miR-34a level and Bcl-2 expression; Bioinformatics and luciferase reporter assays revealed that miR-34a binds the 3'-UTR of the Bcl-2 mRNA and represses its translation; Western blotting analysis and qRT-PCR confirmed that Bcl-2 is inhibited by miR-34a overexpression; Functional analyses indicated that the restoration of miR-34a reduced cell viability promoted cell apoptosis and potentiated sorafenib-induced apoptosis and toxicity in HCC cell lines by inhibiting Bcl-2 expression drug resistance;;;;poor survival;drug resistance;;poor survival;differentiation;poor survival;drug resistance;;; breast cancer;breast cancer;breast cancer;glioblastoma;lung squamous cell cancer;prostate cancer;gastric cancer;gastric cancer;pancreatic cancer;prostate cancer;colon cancer;lung cancer;liver cancer hsa-miR-374a-5p BCL2 -0.239709888246804 0.00309819667153627 -0.895845361347303 1.04475176246219e-07 mirMAP -0.330216657607334 4.44639283995132e-06 NA NA NA hsa-miR-374b-5p BCL2 -0.466781429946153 1.17238311619116e-08 -0.895845361347303 1.04475176246219e-07 mirMAP -0.283877494124907 6.14149967949098e-05 NA NA NA hsa-miR-409-3p BCL2 -0.484157606580319 4.02222513573154e-05 -0.895845361347303 1.04475176246219e-07 mirMAP -0.140997687582626 0.00424621383085417 NA NA NA hsa-miR-429 BCL2 2.84108892259147 3.88520896426515e-64 -0.895845361347303 1.04475176246219e-07 miRNAWalker2_validate;miRTarBase;PITA;mirMAP -0.121974754205047 0.000143492269579093 23999873;26513239;26511969 MiR 429 up regulation induces apoptosis and suppresses invasion by targeting Bcl 2 and SP 1 in esophageal carcinoma; Subsequent Western blotting and luciferase reporter assays showed that miR-429 can bind to putative binding sites within the Bcl-2 and SP1 mRNA 3' untranslated regions UTRs to reduce their expression; Up-regulation of miR-429 inhibits invasion and promotes apoptosis in EC cells by targeting Bcl-2 and SP1; Our findings suggest that Bcl-2 and SP1 may serve as major targets of miR-429;MiR 429 Induces Gastric Carcinoma Cell Apoptosis Through Bcl 2; Here we studied the levels of miR-429 and anti-apoptotic protein Bcl-2 in GC specimens; We performed bioinformatics analyses and used luciferase-reporter assay to analyze the relationship between miR-429 and Bcl-2 in GC cells; MiR-429 levels were significantly decreased and Bcl-2 levels were significantly increased in GC specimens compared to the paired adjacent non-tumor gastric tissue; Moreover the levels of miR-429 and Bcl-2 inversely correlated in GC specimens; Bioinformatics analyses showed that miR-429 targeted the 3'-UTR of Bcl-2 mRNA to inhibit its translation which was confirmed by luciferase-reporter assay;MiR 429 induces apoptosis of glioblastoma cell through Bcl 2; Here we analyzed the levels of miR-429 and anti-apoptotic protein Bcl-2 in GBM specimens; We combined bioinformatics analyses and luciferase reporter assay to determine the relationship between miR-429 and Bcl-2 in GBM cells; We found that miR-429 levels were significantly decreased and Bcl-2 levels were significantly increased in GBM specimens compared to the paired adjacent non-tumor brain tissue; Moreover the levels of miR-429 and Bcl-2 inversely correlated; MiR-429 targeted the 3'-UTR of Bcl-2 mRNA to inhibit its translation ;; esophageal cancer;gastric cancer;glioblastoma hsa-miR-450b-5p BCL2 0.781564228875267 3.90462514315383e-11 -0.895845361347303 1.04475176246219e-07 mirMAP -0.230258059009763 2.05822889351104e-06 NA NA NA hsa-miR-452-5p BCL2 -2.48486455572568 1.24430987974325e-33 -0.895845361347303 1.04475176246219e-07 mirMAP -0.281160548783898 1.07099917549241e-26 NA NA NA hsa-miR-455-5p BCL2 0.284641224662773 0.0357572339594277 -0.895845361347303 1.04475176246219e-07 mirMAP -0.409582744430861 1.37477678088678e-22 NA NA NA hsa-miR-542-3p BCL2 0.568822103820889 8.5699075257651e-07 -0.895845361347303 1.04475176246219e-07 mirMAP -0.198415663601992 7.32195879993002e-05 NA NA NA hsa-miR-582-5p BCL2 0.201134299470983 0.149482495292227 -0.895845361347303 1.04475176246219e-07 PITA -0.224134268204108 9.13307546512698e-08 NA NA NA hsa-miR-590-3p BCL2 1.27438512890514 1.03164723481469e-23 -0.895845361347303 1.04475176246219e-07 miRanda;mirMAP -0.386064412166968 3.36735476522038e-17 NA NA NA hsa-miR-590-5p BCL2 0.746515607868274 8.51300479640125e-09 -0.895845361347303 1.04475176246219e-07 miRanda -0.432407489499855 2.16078449133157e-23 NA NA NA hsa-let-7c-5p BCL2L1 -1.9415540533505 9.55250745007793e-48 0.522792065960586 7.61639356485872e-19 miRNAWalker2_validate;miRTarBase -0.128294871675362 1.88688918547409e-19 20347499 Over-expression of let-7c or let-7g led to a clear decrease of Bcl-xL expression in Huh7 and HepG2 cell lines; Reporter assays revealed direct post-transcriptional regulation involving let-7c or let-7g and the 3'-untranslated region of bcl-xl mRNA liver cancer hsa-let-7g-5p BCL2L1 0.0200025784917734 0.791635298815256 0.522792065960586 7.61639356485872e-19 miRNAWalker2_validate;miRTarBase;miRNATAP -0.126630887025706 3.82717679314587e-06 20347499 Over-expression of let-7c or let-7g led to a clear decrease of Bcl-xL expression in Huh7 and HepG2 cell lines; Reporter assays revealed direct post-transcriptional regulation involving let-7c or let-7g and the 3'-untranslated region of bcl-xl mRNA liver cancer hsa-let-7i-5p BCL2L1 -0.333419326258177 3.56960810786801e-08 0.522792065960586 7.61639356485872e-19 miRNATAP -0.321772345664847 8.06744439816135e-22 NA NA NA hsa-miR-140-5p BCL2L1 -0.755971041589807 9.68060922934542e-14 0.522792065960586 7.61639356485872e-19 PITA;miRanda;miRNATAP -0.178298678075701 1.10325405117987e-19 NA NA NA hsa-miR-505-5p BCL2L1 -0.842640534241897 4.43022473091907e-09 0.522792065960586 7.61639356485872e-19 miRNATAP -0.108734867438394 1.06030624737444e-14 NA NA NA hsa-miR-20a-5p BCL2L11 0.44979911120222 0.000602386964327228 0.294197167540624 6.48706063976289e-05 miRNATAP -0.109458155633012 1.06849975165922e-08 27044389 We found that upregulated expression of miR-17 and miR-20a correlates with lack of pro-apoptotic Bim expression lymphoma hsa-miR-221-3p BCL2L11 -0.317970198201501 0.00910855267413448 0.294197167540624 6.48706063976289e-05 miRNAWalker2_validate -0.179126104880457 1.37009857445217e-18 26503209 Knockdown of miR 221 promotes the cisplatin inducing apoptosis by targeting the BIM Bax/Bak axis in breast cancer breast cancer hsa-miR-24-3p BCL2L11 -0.120123317289941 0.21432376743243 0.294197167540624 6.48706063976289e-05 miRNAWalker2_validate;miRNATAP -0.13029868906498 6.37737361682091e-07 26758252 Onco miR 24 regulates cell growth and apoptosis by targeting BCL2L11 in gastric cancer; In the present study miR-24 was found to be up-regulated while the expression of BCL2L11 was inhibited in tumor tissues of GC; Studies from both in vitro and in vivo shown that miR-24 regulates BCL2L11 expression by directly binding with 3'UTR of mRNA thus promoting cell growth migration while inhibiting cell apoptosis gastric cancer hsa-miR-25-3p BCL2L11 -0.00546790775985073 0.944061771389468 0.294197167540624 6.48706063976289e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.104381938467305 0.00144691697771756 22076535;19486339 MiR 25 regulates apoptosis by targeting Bim in human ovarian cancer; Furthermore luciferase assays demonstrated that Bim was the direct target of miR-25; Finally there was an inverse relationship between Bim and miR-25 expression in ovarian cancer tissues; Taken together these data indicate that miR-25 directly regulates apoptosis by targeting Bim in ovarian cancer and that miR-25 could be a potential therapeutic target for ovarian cancer intervention;In tumors with high expression of this cluster reduced expression of the BH3-only protein Bim a miR-25 target was observed ; ovarian cancer;liver cancer hsa-miR-362-5p BCL2L11 -0.248676340901797 0.0726089851456282 0.294197167540624 6.48706063976289e-05 PITA -0.138984017464157 1.19475231803463e-14 NA NA NA hsa-miR-142-5p BDNF 1.16509303143593 1.96641162209073e-13 -1.60102244727579 3.13378744159785e-14 mirMAP -0.203218784089295 7.99082093515086e-06 NA NA NA hsa-miR-148a-5p BDNF 0.572692271754695 2.14306251522796e-06 -1.60102244727579 3.13378744159785e-14 mirMAP -0.397766884665729 3.21732972253681e-11 NA NA NA hsa-miR-155-5p BDNF 1.09766314984151 4.55650930885814e-15 -1.60102244727579 3.13378744159785e-14 miRNATAP -0.268404245015568 1.69880913103772e-07 NA NA NA hsa-miR-15a-5p BDNF 0.812445324157528 4.50263417099822e-20 -1.60102244727579 3.13378744159785e-14 MirTarget;miRNATAP -0.270462236614116 0.000896358870417413 26581909 MicroRNA 15a 5p suppresses cancer proliferation and division in human hepatocellular carcinoma by targeting BDNF; BDNF was then overexpressed in HepG2 and SNU-182 cells to evaluate its selective effect on miR-15a-5p in HCC modulation; MiR-15a-5p selectively and negatively regulated BDNF at both gene and protein levels in HCC cells; Forced overexpression of BDNF effectively reversed the tumor suppressive functions of miR-15a-5p on HCC proliferation and cell division in vitro; Our study demonstrated that miR-15a-5p is a tumor suppressor in HCC and its regulation is through BDNF in HCC liver cancer hsa-miR-16-5p BDNF 0.46835991343556 1.91125356547776e-06 -1.60102244727579 3.13378744159785e-14 MirTarget;miRNATAP -0.207572734514068 0.0054038108129975 NA NA NA hsa-miR-182-5p BDNF 2.36408084103818 2.60011457600281e-72 -1.60102244727579 3.13378744159785e-14 MirTarget;miRNATAP -0.234588678348713 3.99165896393269e-06 NA NA NA hsa-miR-210-3p BDNF 2.38367203491819 3.73261999106754e-22 -1.60102244727579 3.13378744159785e-14 miRNAWalker2_validate;miRTarBase -0.115495455176462 6.58638340303894e-05 NA NA NA hsa-miR-30e-5p BDNF 0.293491764699475 0.000372291967271834 -1.60102244727579 3.13378744159785e-14 miRNATAP -0.400421315846613 7.13698491153316e-06 NA NA NA hsa-miR-589-3p BDNF 0.0263457630961427 0.866577983997705 -1.60102244727579 3.13378744159785e-14 MirTarget -0.125027718317622 0.00920347953748705 NA NA NA hsa-miR-589-5p BDNF 0.367182541239722 1.67804815542873e-05 -1.60102244727579 3.13378744159785e-14 miRNATAP -0.314299850428925 0.00026517944556323 NA NA NA hsa-miR-590-3p BDNF 1.27438512890514 1.03164723481469e-23 -1.60102244727579 3.13378744159785e-14 miRanda;mirMAP;miRNATAP -0.314287602443717 7.91546458232199e-08 NA NA NA hsa-miR-7-1-3p BDNF 0.686579567507582 1.20731554448806e-07 -1.60102244727579 3.13378744159785e-14 MirTarget -0.157643440663764 0.00510952033279118 NA NA NA hsa-miR-199a-5p BRCA1 -0.314703593105081 0.00909304983051124 0.7891100224999 1.35168321783973e-11 miRanda -0.140483934692518 2.74607603656248e-05 NA NA NA hsa-miR-199b-5p BRCA1 -1.37022396448377 3.25758057539223e-26 0.7891100224999 1.35168321783973e-11 miRanda -0.205594262694619 7.40215872368304e-12 NA NA NA hsa-miR-320a BRCA1 -0.287921276129346 0.00141693637596294 0.7891100224999 1.35168321783973e-11 miRanda -0.120889738239334 0.00729578780648754 NA NA NA hsa-miR-34c-5p BRCA1 -0.0896438810345765 0.557016551646841 0.7891100224999 1.35168321783973e-11 miRanda -0.106203229169271 6.52743183568861e-05 NA NA NA hsa-miR-17-5p CASP9 0.599531757298529 1.07318660415906e-05 0.0553003248250161 0.30285132191595 TargetScan -0.101631873053047 1.40876150138688e-14 NA NA NA hsa-let-7i-5p CCND1 -0.333419326258177 3.56960810786801e-08 0.789100261201456 1.48424202389562e-06 miRNATAP -0.759700962013673 2.31065071397228e-16 NA NA NA hsa-miR-106a-5p CCND1 0.574594373206545 0.000145868284001732 0.789100261201456 1.48424202389562e-06 MirTarget;miRNATAP -0.174359830876255 2.65007775906387e-06 NA NA NA hsa-miR-106b-5p CCND1 1.22477789913175 2.31787213143326e-32 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.258644062815507 8.24041501542474e-07 NA NA NA hsa-miR-142-3p CCND1 1.53951237846104 2.06769936282034e-17 0.789100261201456 1.48424202389562e-06 miRanda -0.111966511365121 0.000240262100008297 23619912 Transfection of miR-142-3p mimics in colon cancer cells downregulated cyclin D1 expression induced G1 phase cell cycle arrest and elevated the sensitivity of the cells to 5-fluorouracil colon cancer hsa-miR-142-5p CCND1 1.16509303143593 1.96641162209073e-13 0.789100261201456 1.48424202389562e-06 PITA -0.210173449112337 1.63510921192815e-09 NA NA NA hsa-miR-150-5p CCND1 -0.832049526091211 3.45299201139224e-05 0.789100261201456 1.48424202389562e-06 mirMAP -0.187252742453937 1.34917625164856e-11 NA NA NA hsa-miR-155-5p CCND1 1.09766314984151 4.55650930885814e-15 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate -0.298681895389198 2.32439253453393e-14 26955820 MicroRNA 155 expression inversely correlates with pathologic stage of gastric cancer and it inhibits gastric cancer cell growth by targeting cyclin D1 staging gastric cancer hsa-miR-15b-5p CCND1 0.788659356634696 1.13678211092534e-11 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.125463191460891 0.00921827566968511 NA NA NA hsa-miR-17-5p CCND1 0.599531757298529 1.07318660415906e-05 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.23795787679729 6.72139201694027e-09 26431674 Bioinformatics Prediction and In Vitro Analysis Revealed That miR 17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells; In this study using bioinformatic analyses miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score; After lentiviral transduction of miR-17 to the target cells gene expression analysis showed decreased expression of CCND1 gene breast cancer hsa-miR-186-5p CCND1 -0.426987301460247 1.04129380549315e-05 0.789100261201456 1.48424202389562e-06 mirMAP -0.494988902443749 5.18517439622754e-18 NA NA NA hsa-miR-19a-3p CCND1 0.817507952171848 3.76285090469625e-08 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.216023850578105 8.24189814452405e-09 25985117 Moreover miR-19a might play inhibitory roles in HCC malignancy via regulating Cyclin D1 expression liver cancer hsa-miR-19b-1-5p CCND1 0.647704962003062 3.07687667945597e-07 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase -0.206802040114629 3.03348057195458e-06 NA NA NA hsa-miR-19b-3p CCND1 0.139124391010031 0.255658553872934 0.789100261201456 1.48424202389562e-06 miRNATAP -0.249444343540709 5.93160032007176e-08 NA NA NA hsa-miR-20a-5p CCND1 0.44979911120222 0.000602386964327228 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.218045690926451 3.58399990050218e-07 NA NA NA hsa-miR-23b-3p CCND1 -0.363202401123049 0.000265305319764878 0.789100261201456 1.48424202389562e-06 miRNATAP -0.2085521275068 0.000237936656088415 NA NA NA hsa-miR-29a-3p CCND1 -0.699539666833076 1.13935003139957e-13 0.789100261201456 1.48424202389562e-06 mirMAP -0.358623457755512 1.12263469726765e-09 NA NA NA hsa-miR-340-5p CCND1 1.0857159627596 4.94471062546756e-24 0.789100261201456 1.48424202389562e-06 mirMAP -0.157803294541288 0.00210629660665075 NA NA NA hsa-miR-374a-5p CCND1 -0.239709888246804 0.00309819667153627 0.789100261201456 1.48424202389562e-06 MirTarget -0.556352001430099 7.67413515717595e-16 27191497 microRNA 374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway; Furthermore luciferase reporter assays confirmed that miR-374a could directly reduce CCND1; We examined miR-374a levels by in situ hybridization and its correlation with CCND1 expression in CRC tumor tissues; High miR-374a expression with low level of CCND1 was protective factor in CRC; Together these findings indicate that miR-374a inactivates the PI3K/AKT axis by inhibiting CCND1 suppressing of colon cancer progression progression colon cancer hsa-miR-374b-5p CCND1 -0.466781429946153 1.17238311619116e-08 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;MirTarget -0.531193112029599 5.3445494840887e-15 NA NA NA hsa-miR-424-5p CCND1 0.420789584199235 0.00480101660479489 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.165346668007668 1.17175782770906e-05 NA NA NA hsa-miR-495-3p CCND1 -1.67685437612894 1.70634429769052e-34 0.789100261201456 1.48424202389562e-06 MirTarget -0.2655760978611 1.39010362325337e-11 NA NA NA hsa-miR-511-5p CCND1 -2.27545475897362 3.84085514050558e-36 0.789100261201456 1.48424202389562e-06 MirTarget -0.21938803198833 1.27831310404617e-13 NA NA NA hsa-miR-589-3p CCND1 0.0263457630961427 0.866577983997705 0.789100261201456 1.48424202389562e-06 MirTarget -0.116809863299678 0.00155105242627909 NA NA NA hsa-miR-590-3p CCND1 1.27438512890514 1.03164723481469e-23 0.789100261201456 1.48424202389562e-06 mirMAP -0.221641713536578 8.94498108920728e-07 NA NA NA hsa-miR-9-5p CCND1 0.734772328078424 0.00261982843636383 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate -0.162121975003827 1.12937727530253e-12 NA NA NA hsa-miR-92a-3p CCND1 -0.342420481092807 0.000732275969501111 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate -0.355039027931141 1.3665845638516e-10 NA NA NA hsa-miR-93-5p CCND1 1.01788600209314 1.24312921618126e-21 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;MirTarget;miRNATAP -0.160469090487963 0.00197437840989499 NA NA NA hsa-miR-942-5p CCND1 0.0274758353801279 0.842368857038259 0.789100261201456 1.48424202389562e-06 MirTarget -0.261175156275166 1.39422778172566e-10 NA NA NA hsa-let-7a-3p CCND2 0.466622567891891 3.70886257943079e-07 -1.32381712777542 1.10807863083966e-33 mirMAP -0.215275364862757 3.63993039775257e-07 20418948 MicroRNA let 7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2 prostate cancer hsa-let-7e-5p CCND2 0.261471269338985 0.00191077095550257 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.168033346737038 0.000309648297817392 NA NA NA hsa-miR-106a-5p CCND2 0.574594373206545 0.000145868284001732 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.117204496646304 5.04333413242193e-06 NA NA NA hsa-miR-106b-5p CCND2 1.22477789913175 2.31787213143326e-32 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.324304654054346 9.75129599972944e-20 NA NA NA hsa-miR-130b-5p CCND2 1.0573161199584 2.55177349228739e-12 -1.32381712777542 1.10807863083966e-33 mirMAP -0.113060251982174 8.9777676148938e-06 NA NA NA hsa-miR-141-3p CCND2 2.99777540204168 1.26370498493024e-78 -1.32381712777542 1.10807863083966e-33 MirTarget;TargetScan -0.300723945575078 8.35441899721039e-47 NA NA NA hsa-miR-151a-3p CCND2 0.3530403796941 3.45015092968892e-05 -1.32381712777542 1.10807863083966e-33 mirMAP -0.17767569994557 0.000108302525662849 NA NA NA hsa-miR-15a-5p CCND2 0.812445324157528 4.50263417099822e-20 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.382989347535057 1.85935181542133e-19 NA NA NA hsa-miR-15b-5p CCND2 0.788659356634696 1.13678211092534e-11 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.200863767053961 1.18885859507843e-09 NA NA NA hsa-miR-16-5p CCND2 0.46835991343556 1.91125356547776e-06 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRNATAP -0.126011969570663 0.00149555991801246 NA NA NA hsa-miR-17-5p CCND2 0.599531757298529 1.07318660415906e-05 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.218141508896336 9.56625930755479e-15 NA NA NA hsa-miR-182-5p CCND2 2.36408084103818 2.60011457600281e-72 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.333755066801542 7.98233999507297e-38 NA NA NA hsa-miR-183-5p CCND2 2.95681814393872 1.09965777344285e-92 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.301857921065621 1.16754966176673e-39 NA NA NA hsa-miR-185-5p CCND2 0.482334746589337 3.68475879959415e-09 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.252091965164496 1.07493887045353e-07 NA NA NA hsa-miR-191-5p CCND2 0.831652724468215 1.98847993606895e-12 -1.32381712777542 1.10807863083966e-33 MirTarget -0.190841372088135 4.05600831845751e-09 NA NA NA hsa-miR-19a-3p CCND2 0.817507952171848 3.76285090469625e-08 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.185131062383478 6.94387115240903e-13 NA NA NA hsa-miR-19b-3p CCND2 0.139124391010031 0.255658553872934 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;MirTarget;miRNATAP -0.166767747836903 1.62862778749919e-07 NA NA NA hsa-miR-200a-3p CCND2 2.55218480823434 2.96135446816238e-51 -1.32381712777542 1.10807863083966e-33 MirTarget -0.261226562912324 4.31939405604759e-36 NA NA NA hsa-miR-20a-5p CCND2 0.44979911120222 0.000602386964327228 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.235095611327971 1.01223581717651e-15 NA NA NA hsa-miR-20b-5p CCND2 0.78236583455125 8.7938334801223e-05 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.104708964184932 6.77204420924901e-08 NA NA NA hsa-miR-21-3p CCND2 2.17476065946878 5.15299442583591e-75 -1.32381712777542 1.10807863083966e-33 mirMAP -0.127404398971718 2.08059143790106e-05 NA NA NA hsa-miR-29b-3p CCND2 0.438847962603729 6.46722433236121e-05 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.166630108966211 2.5453033344596e-06 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-301a-3p CCND2 1.32170168375632 2.12280368957514e-15 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate -0.182363632693643 1.4299291622836e-15 NA NA NA hsa-miR-3065-3p CCND2 1.62787885002468 6.81477830005026e-19 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.215305118861858 1.68654607913855e-26 NA NA NA hsa-miR-30d-3p CCND2 0.264544633563642 0.00755023189129747 -1.32381712777542 1.10807863083966e-33 mirMAP -0.277305057640272 1.40644960106378e-12 NA NA NA hsa-miR-320b CCND2 0.412787504747938 0.00113457276903985 -1.32381712777542 1.10807863083966e-33 mirMAP;miRNATAP -0.105338094438455 0.000662995774848357 NA NA NA hsa-miR-324-3p CCND2 0.404877731996915 0.000340036423920834 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate -0.187482582074807 4.91214943402943e-08 NA NA NA hsa-miR-331-5p CCND2 0.682437632502949 4.10871642662371e-11 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.224823939820064 1.69348201302645e-09 NA NA NA hsa-miR-342-3p CCND2 1.21944481975657 6.81914373001202e-19 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;mirMAP -0.103848624831325 0.000189231379572542 NA NA NA hsa-miR-429 CCND2 2.84108892259147 3.88520896426515e-64 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.232180070081619 1.04111918722712e-28 NA NA NA hsa-miR-590-3p CCND2 1.27438512890514 1.03164723481469e-23 -1.32381712777542 1.10807863083966e-33 miRanda;mirMAP -0.13833737728579 9.4871454586344e-06 NA NA NA hsa-miR-590-5p CCND2 0.746515607868274 8.51300479640125e-09 -1.32381712777542 1.10807863083966e-33 mirMAP -0.151134124586935 3.93710909950627e-07 NA NA NA hsa-miR-660-5p CCND2 0.406910761707485 0.000156175371762464 -1.32381712777542 1.10807863083966e-33 mirMAP -0.106401121539045 0.00339848496849202 NA NA NA hsa-miR-7-1-3p CCND2 0.686579567507582 1.20731554448806e-07 -1.32381712777542 1.10807863083966e-33 mirMAP -0.138929082076826 3.22847149980611e-06 NA NA NA hsa-miR-93-5p CCND2 1.01788600209314 1.24312921618126e-21 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.400435186075896 5.37578279205249e-31 NA NA NA hsa-miR-96-5p CCND2 3.24596558166606 1.92296159041707e-98 -1.32381712777542 1.10807863083966e-33 TargetScan;miRNATAP -0.311897703713413 7.58506265459077e-49 NA NA NA hsa-miR-125b-5p CCNE1 -1.94393269763542 2.78180543229264e-58 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate -0.382224070637194 4.63212335978261e-14 NA NA NA hsa-miR-195-5p CCNE1 -1.47508108420708 2.20685847368258e-38 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate;MirTarget;miRNATAP -0.617319346276305 3.09483424185554e-30 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.481180084102235 1.82368246174758e-10 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate;miRTarBase;miRNATAP -0.619294201366171 1.19374874415568e-12 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-26b-5p CCNE1 -0.331527896896866 0.000551258576575092 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate;miRTarBase;miRNATAP -0.319970519742405 4.01394740915552e-06 NA NA NA hsa-miR-342-5p CCNE1 1.49554286219821 1.56221300255782e-22 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate -0.135292830202672 0.00141592689314045 NA NA NA hsa-miR-497-5p CCNE1 -1.4667331575679 5.49909921822638e-36 2.02272205094151 6.47439339208807e-27 MirTarget;miRNATAP -0.382195878498221 1.36469362381851e-12 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-26a-5p CCNE2 -0.481180084102235 1.82368246174758e-10 2.57138773577447 1.5602638336465e-57 miRNAWalker2_validate;miRTarBase;miRNATAP -0.630654803219519 2.81902449356968e-16 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-28-3p CCNE2 -0.815347900986328 3.29387468083828e-30 2.57138773577447 1.5602638336465e-57 PITA;miRNATAP -0.332464341790485 3.74937420435342e-05 NA NA NA hsa-miR-30a-5p CCNE2 -0.392489994825279 0.00954894667761676 2.57138773577447 1.5602638336465e-57 miRNATAP -0.389839068904493 1.17236295202714e-24 NA NA NA hsa-miR-30c-5p CCNE2 -0.393222674580066 8.06961465717306e-05 2.57138773577447 1.5602638336465e-57 miRNATAP -0.253921413883948 1.85400203918647e-05 NA NA NA hsa-miR-335-3p CCNE2 -1.88448538773649 2.09538234398335e-32 2.57138773577447 1.5602638336465e-57 mirMAP -0.16561143561413 3.48668881373491e-06 NA NA NA hsa-miR-34c-5p CCNE2 -0.0896438810345765 0.557016551646841 2.57138773577447 1.5602638336465e-57 miRNAWalker2_validate;miRTarBase;PITA;miRanda;miRNATAP -0.253783325617594 5.10732275535071e-11 NA NA NA hsa-miR-331-3p CDC37 0.74441480542886 1.17071235477799e-07 0.342951115410673 2.88753198580336e-06 miRNATAP -0.146025745791985 7.76032126619259e-17 NA NA NA hsa-miR-10b-3p CDK2 -1.58463104907636 1.17335119858099e-46 0.628977809366634 3.17063810755044e-21 mirMAP -0.210836970989172 1.24690584925124e-27 NA NA NA hsa-miR-10b-5p CDK2 -2.10996264627796 7.36811595736367e-72 0.628977809366634 3.17063810755044e-21 miRNAWalker2_validate -0.172779155677722 1.52256449776603e-22 NA NA NA hsa-miR-145-5p CDK4 -2.62703571936687 7.64402900188551e-88 0.597969315491349 4.23701383421285e-22 miRNAWalker2_validate;miRTarBase -0.134364969525933 9.65405723067241e-21 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-195-5p CDK4 -1.47508108420708 2.20685847368258e-38 0.597969315491349 4.23701383421285e-22 miRNAWalker2_validate;miRTarBase -0.168636406260907 2.79019414251395e-21 NA NA NA hsa-miR-103a-3p CDK6 0.610673390034345 1.63113920514646e-12 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRNATAP -0.41897711298053 2.13541829274935e-12 NA NA NA hsa-miR-107 CDK6 0.678998026350662 2.56224148930538e-16 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.220761526250824 0.000423296532800194 19407485;22491216;21264532;19688090 Enforced expression of miR-107 in MiaPACA-2 and PANC-1 cells downregulated in vitro growth and this was associated with repression of the putative miR-107 target cyclin-dependent kinase 6 thereby providing a functional basis for the epigenetic inactivation of this miRNA in pancreatic cancer;Levels of known miR-107 targets protein kinase Cε PKCε cyclin-dependent kinase 6 CDK6 and hypoxia-inducible factor 1-β HIF1-β decreased following NP/pre-miR-107 treatment;We have identified miR-107 as a potential regulator of CDK6 expression; A bioinformatics search revealed a putative target site for miR-107 within the CDK6 3' untranslated region; Expression of miR-107 in gastric cancer cell lines was found inversely correlated with CDK6 expression; miR-107 could significantly suppress CDK6 3' UTR luciferase reporter activity and this effect was not detectable when the putative 3' UTR target site was mutated; Consistent with the results of the reporter assay ectopic expression of miR-107 reduced both mRNA and protein expression levels of CDK6 inhibited proliferation induced G1 cell cycle arrest and blocked invasion of the gastric cancer cells; Our results suggest that miR-107 may have a tumor suppressor function by directly targeting CDK6 to inhibit the proliferation and invasion activities of gastric cancer cells;Using miRNA-target prediction analyses and the array data we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6 ;;; pancreatic cancer;head and neck cancer;gastric cancer;lung squamous cell cancer hsa-miR-141-3p CDK6 2.99777540204168 1.26370498493024e-78 -1.03099881099472 9.88361268912926e-12 TargetScan;miRNATAP -0.155074529575108 1.68227129058067e-07 NA NA NA hsa-miR-15a-5p CDK6 0.812445324157528 4.50263417099822e-20 -1.03099881099472 9.88361268912926e-12 miRNATAP -0.307441214732404 1.1453244726092e-07 NA NA NA hsa-miR-182-5p CDK6 2.36408084103818 2.60011457600281e-72 -1.03099881099472 9.88361268912926e-12 mirMAP -0.302395516858316 3.47976531991082e-17 NA NA NA hsa-miR-191-5p CDK6 0.831652724468215 1.98847993606895e-12 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRTarBase -0.206787526199797 2.32979427977823e-06 NA NA NA hsa-miR-193b-3p CDK6 -0.0550374855074773 0.65382485898551 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate -0.155046597813103 0.00030084184892796 NA NA NA hsa-miR-200a-3p CDK6 2.55218480823434 2.96135446816238e-51 -1.03099881099472 9.88361268912926e-12 miRNATAP -0.153245463849065 1.36473748839668e-07 24009066 microRNA 200a is an independent prognostic factor of hepatocellular carcinoma and induces cell cycle arrest by targeting CDK6 liver cancer hsa-miR-200b-3p CDK6 2.11744072703209 1.66710572104191e-43 -1.03099881099472 9.88361268912926e-12 mirMAP -0.174815127052701 6.39294256795547e-08 NA NA NA hsa-miR-200c-3p CDK6 2.07038651806183 6.84613386286599e-51 -1.03099881099472 9.88361268912926e-12 mirMAP -0.180928310145507 3.68922155138771e-07 NA NA NA hsa-miR-21-5p CDK6 2.32007501846389 3.08394781855554e-137 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;mirMAP -0.197449989063307 3.4492907261557e-05 NA NA NA hsa-miR-29c-3p CDK6 -0.0652732378039769 0.563774115756922 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRTarBase;miRNATAP -0.390844229843503 1.3743800981758e-17 26396669 Furthermore through qPCR and Western blot assays confirmed that overexpression of miR-29c reduced CDK6 mRNA and protein levels; miR-29c could inhibit the proliferation migration and invasion of bladder cancer cells via regulating CDK6 bladder cancer hsa-miR-30a-5p CDK6 -0.392489994825279 0.00954894667761676 -1.03099881099472 9.88361268912926e-12 mirMAP -0.124807010230796 0.000314782970367504 NA NA NA hsa-miR-338-3p CDK6 0.0799903526884966 0.575137668326679 -1.03099881099472 9.88361268912926e-12 mirMAP -0.191800207428879 1.71408750980913e-07 NA NA NA hsa-miR-425-5p CDK6 0.719898949241718 6.01079610190374e-08 -1.03099881099472 9.88361268912926e-12 mirMAP -0.150921506027425 0.000119900868002377 NA NA NA hsa-miR-429 CDK6 2.84108892259147 3.88520896426515e-64 -1.03099881099472 9.88361268912926e-12 mirMAP;miRNATAP -0.133862145668 3.72317905758963e-06 NA NA NA hsa-miR-664a-3p CDK6 -0.237699857923843 0.0197071390183375 -1.03099881099472 9.88361268912926e-12 mirMAP -0.195343666542438 0.000155666654807136 NA NA NA hsa-miR-92b-3p CDK6 1.30248132773644 9.42428674470985e-25 -1.03099881099472 9.88361268912926e-12 miRNATAP -0.108885215584239 0.00691585930585228 NA NA NA hsa-let-7d-5p CDKN1A 0.410958462607207 6.07808182756787e-06 -0.254319078213554 0.025069242968496 MirTarget -0.209534907854426 9.05682787226141e-07 NA NA NA hsa-let-7g-5p CDKN1A 0.0200025784917734 0.791635298815256 -0.254319078213554 0.025069242968496 MirTarget -0.234754189393088 5.7315667018148e-06 NA NA NA hsa-miR-101-3p CDKN1A -0.126035286271986 0.145997832808138 -0.254319078213554 0.025069242968496 MirTarget -0.12547684489979 0.00551360058669363 NA NA NA hsa-miR-106a-5p CDKN1A 0.574594373206545 0.000145868284001732 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.114530864663224 7.53068059714178e-06 25510666 After prediction with online software we further used dual-luciferase reporter gene assay to ensure that TP53INP1 and CDKN1A might be the direct targets of miR-106a liver cancer hsa-miR-106b-5p CDKN1A 1.22477789913175 2.31787213143326e-32 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.198769219405748 3.53449473460544e-08 NA NA NA hsa-miR-146a-5p CDKN1A 0.102405752365098 0.486975378688068 -0.254319078213554 0.025069242968496 miRNAWalker2_validate -0.112996377410099 1.83135810400461e-05 NA NA NA hsa-miR-17-5p CDKN1A 0.599531757298529 1.07318660415906e-05 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.157707858341151 2.444032078598e-08 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-182-5p CDKN1A 2.36408084103818 2.60011457600281e-72 -0.254319078213554 0.025069242968496 miRNAWalker2_validate -0.114208671947908 2.29748891177513e-05 NA NA NA hsa-miR-20a-5p CDKN1A 0.44979911120222 0.000602386964327228 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.126075559540983 1.99860276007902e-05 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-28-5p CDKN1A -0.14267073663385 0.0497619246260571 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRanda;miRNATAP -0.294350864895331 4.29596264827348e-08 NA NA NA hsa-miR-423-3p CDKN1A 0.338351552541003 0.000947983901628776 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase -0.105368362155166 0.00567064832994105 NA NA NA hsa-miR-455-3p CDKN1A 0.678392920628872 0.000327758844555877 -0.254319078213554 0.025069242968496 MirTarget -0.119773167671374 4.14708070797368e-09 NA NA NA hsa-miR-505-5p CDKN1A -0.842640534241897 4.43022473091907e-09 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;MirTarget -0.108248027724388 5.69419612511767e-05 NA NA NA hsa-miR-93-5p CDKN1A 1.01788600209314 1.24312921618126e-21 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.147676795890733 3.39439379040982e-05 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 0.0274758353801279 0.842368857038259 -0.254319078213554 0.025069242968496 miRNAWalker2_validate -0.114405823345184 5.05622411237802e-05 NA NA NA hsa-miR-96-5p CDKN1A 3.24596558166606 1.92296159041707e-98 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase -0.102209126041297 4.86561723152027e-06 26582573 Upregulation of microRNA 96 and its oncogenic functions by targeting CDKN1A in bladder cancer; Bioinformatics prediction combined with luciferase reporter assay were used to verify whether the cyclin-dependent kinase inhibitor CDKN1A was a potential target gene of miR-96; According to the data of miRTarBase CDKN1A might be a candidate target gene of miR-96; In addition luciferase reporter and Western blot assays respectively demonstrated that miR-96 could bind to the putative seed region in CDKN1A mRNA 3'UTR and significantly reduce the expression level of CDKN1A protein; Moreover we found that the inhibition of miR-96 expression remarkably decreased cell proliferation and promoted cell apoptosis of BC cell lines which was consistent with the findings observed following the introduction of CDKN1A cDNA without 3'UTR restored miR-96; Upregulation of miR-96 may contribute to aggressive malignancy partly through suppressing CDKN1A protein expression in BC cells bladder cancer hsa-miR-98-5p CDKN1A 0.656336869298973 9.03113188590466e-15 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;MirTarget -0.175027497589803 0.000116761897589387 NA NA NA hsa-miR-24-3p CDKN1B -0.120123317289941 0.21432376743243 0.123956597380626 0.178678734172075 miRNAWalker2_validate;miRNATAP -0.129882054588421 7.20288249970178e-05 26044523;26847530 With the bioinformatic method we further identified that p27Kip1 is a direct target of miR-24-3p and its protein level was negatively regulated by miR-24-3p;The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated ; breast cancer;prostate cancer hsa-miR-505-3p CDKN1B -0.530462599544864 7.71645736541597e-05 0.123956597380626 0.178678734172075 MirTarget -0.109145829867351 2.9885589878634e-06 NA NA NA hsa-miR-107 CHAD 0.678998026350662 2.56224148930538e-16 -0.0030842411669636 0.993051648329624 miRanda -0.675138251853785 2.80905562833419e-06 NA NA NA hsa-miR-222-5p CHAD -0.0126640010215462 0.929279244700619 -0.0030842411669636 0.993051648329624 MirTarget -0.655462590929912 6.71448355873324e-15 NA NA NA hsa-miR-28-5p CHAD -0.14267073663385 0.0497619246260571 -0.0030842411669636 0.993051648329624 miRanda -0.898299919129268 8.28846753994342e-08 NA NA NA hsa-miR-342-3p CHRM1 1.21944481975657 6.81914373001202e-19 -1.50782427768987 9.76057401934574e-07 miRanda -0.43302880975582 8.38402354412908e-09 NA NA NA hsa-miR-15a-5p CHUK 0.812445324157528 4.50263417099822e-20 -0.0655383466936339 0.252025603609673 miRNAWalker2_validate;miRTarBase;MirTarget -0.135155500445723 3.1799004977298e-10 NA NA NA hsa-let-7a-5p COL1A1 -0.210286642041636 0.0072546234707717 2.5651762209113 9.3730343879076e-47 TargetScan;miRNATAP -0.24089218391089 0.00393524214991794 NA NA NA hsa-let-7b-5p COL1A1 -0.54215483448257 7.21347617597858e-08 2.5651762209113 9.3730343879076e-47 miRNATAP -0.208318456681387 0.00118621429715631 NA NA NA hsa-let-7g-5p COL1A1 0.0200025784917734 0.791635298815256 2.5651762209113 9.3730343879076e-47 miRNATAP -0.502387292694151 5.2158427786542e-09 NA NA NA hsa-miR-126-5p COL1A1 -1.27745588751177 2.96000918537631e-30 2.5651762209113 9.3730343879076e-47 miRNATAP -0.431075814120194 6.33610324209054e-15 NA NA NA hsa-miR-17-5p COL1A1 0.599531757298529 1.07318660415906e-05 2.5651762209113 9.3730343879076e-47 mirMAP -0.279131452817333 3.18067245709467e-09 NA NA NA hsa-miR-193a-5p COL1A1 -1.1508321886378 7.11949564429669e-37 2.5651762209113 9.3730343879076e-47 PITA;TargetScan;miRNATAP -0.419754083355121 7.32652492145808e-10 NA NA NA hsa-miR-20a-5p COL1A1 0.44979911120222 0.000602386964327228 2.5651762209113 9.3730343879076e-47 mirMAP -0.264687400603912 7.34043720394447e-08 NA NA NA hsa-miR-29a-3p COL1A1 -0.699539666833076 1.13935003139957e-13 2.5651762209113 9.3730343879076e-47 miRNATAP -0.505920263846829 5.4026826839022e-14 NA NA NA hsa-miR-29b-3p COL1A1 0.438847962603729 6.46722433236121e-05 2.5651762209113 9.3730343879076e-47 miRNAWalker2_validate;miRTarBase;miRNATAP -0.42483491870975 3.19558825282899e-13 NA NA NA hsa-miR-29c-3p COL1A1 -0.0652732378039769 0.563774115756922 2.5651762209113 9.3730343879076e-47 miRNAWalker2_validate;miRTarBase;miRNATAP -0.308413017460321 7.31731652067127e-08 NA NA NA hsa-miR-30a-3p COL1A1 -1.08956435286856 1.10750674569595e-12 2.5651762209113 9.3730343879076e-47 mirMAP -0.293364132995892 1.26433028895325e-12 NA NA NA hsa-miR-30b-3p COL1A1 -0.536462824497982 2.41814659272548e-05 2.5651762209113 9.3730343879076e-47 miRNATAP -0.444357683038922 9.1689823110698e-19 NA NA NA hsa-miR-30d-3p COL1A1 0.264544633563642 0.00755023189129747 2.5651762209113 9.3730343879076e-47 mirMAP -0.429158337602994 4.53272123771336e-11 NA NA NA hsa-miR-30e-3p COL1A1 -0.708952170420973 1.05866470714978e-22 2.5651762209113 9.3730343879076e-47 mirMAP -0.766163072983284 8.35863907266093e-19 NA NA NA hsa-miR-338-3p COL1A1 0.0799903526884966 0.575137668326679 2.5651762209113 9.3730343879076e-47 miRanda;miRNATAP -0.207676895625599 4.80091970867201e-06 NA NA NA hsa-miR-361-3p COL1A1 -0.0370446538644886 0.624902392829872 2.5651762209113 9.3730343879076e-47 PITA -0.464593125962225 6.76797418412615e-08 NA NA NA hsa-miR-532-3p COL1A1 -0.395473145042459 0.00686714288452761 2.5651762209113 9.3730343879076e-47 PITA;miRNATAP -0.295814858390006 1.57092989055696e-11 NA NA NA hsa-let-7a-3p COL1A2 0.466622567891891 3.70886257943079e-07 1.52331001708955 5.43111193537147e-20 MirTarget -0.190581165353783 0.00270286404978591 NA NA NA hsa-let-7a-5p COL1A2 -0.210286642041636 0.0072546234707717 1.52331001708955 5.43111193537147e-20 MirTarget;TargetScan;miRNATAP -0.194876554818876 0.00952052671105555 NA NA NA hsa-let-7g-5p COL1A2 0.0200025784917734 0.791635298815256 1.52331001708955 5.43111193537147e-20 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.461558685623361 2.37786040489822e-09 NA NA NA hsa-miR-125a-5p COL1A2 -0.635694700253268 3.6703235721638e-11 1.52331001708955 5.43111193537147e-20 miRanda -0.21480066787771 0.0003657222089883 NA NA NA hsa-miR-19a-3p COL1A2 0.817507952171848 3.76285090469625e-08 1.52331001708955 5.43111193537147e-20 MirTarget -0.274114501964427 1.11751402004845e-12 NA NA NA hsa-miR-19b-3p COL1A2 0.139124391010031 0.255658553872934 1.52331001708955 5.43111193537147e-20 MirTarget -0.454699809629064 1.84023698280234e-22 NA NA NA hsa-miR-25-3p COL1A2 -0.00546790775985073 0.944061771389468 1.52331001708955 5.43111193537147e-20 MirTarget;miRNATAP -0.55199742436697 1.4211368225352e-13 NA NA NA hsa-miR-26a-5p COL1A2 -0.481180084102235 1.82368246174758e-10 1.52331001708955 5.43111193537147e-20 MirTarget;miRNATAP -0.442417015562675 7.96979615182122e-09 NA NA NA hsa-miR-26b-5p COL1A2 -0.331527896896866 0.000551258576575092 1.52331001708955 5.43111193537147e-20 MirTarget;miRNATAP -0.291841349110864 1.54077222441719e-06 NA NA NA hsa-miR-29a-3p COL1A2 -0.699539666833076 1.13935003139957e-13 1.52331001708955 5.43111193537147e-20 miRNATAP -0.454797163293173 5.49456065194944e-14 NA NA NA hsa-miR-29b-3p COL1A2 0.438847962603729 6.46722433236121e-05 1.52331001708955 5.43111193537147e-20 miRNATAP -0.460605295650497 8.76503901098457e-19 NA NA NA hsa-miR-29c-3p COL1A2 -0.0652732378039769 0.563774115756922 1.52331001708955 5.43111193537147e-20 miRNAWalker2_validate;miRTarBase;miRNATAP -0.366812628404496 7.65487887214513e-13 NA NA NA hsa-miR-30a-3p COL1A2 -1.08956435286856 1.10750674569595e-12 1.52331001708955 5.43111193537147e-20 MirTarget -0.196375843318247 1.53150260349314e-07 NA NA NA hsa-miR-30d-3p COL1A2 0.264544633563642 0.00755023189129747 1.52331001708955 5.43111193537147e-20 MirTarget -0.449068692307375 1.36798508181843e-14 NA NA NA hsa-miR-30e-3p COL1A2 -0.708952170420973 1.05866470714978e-22 1.52331001708955 5.43111193537147e-20 MirTarget -0.437169418077207 3.00568168055859e-08 NA NA NA hsa-miR-500a-5p COL1A2 0.209805801129289 0.119177819862398 1.52331001708955 5.43111193537147e-20 miRNATAP -0.164084171128344 0.000189697270220366 NA NA NA hsa-miR-590-3p COL1A2 1.27438512890514 1.03164723481469e-23 1.52331001708955 5.43111193537147e-20 miRanda -0.197917414728781 2.24242453532176e-05 NA NA NA hsa-miR-7-1-3p COL1A2 0.686579567507582 1.20731554448806e-07 1.52331001708955 5.43111193537147e-20 mirMAP -0.217497538560182 1.04875229508942e-06 NA NA NA hsa-miR-92a-3p COL1A2 -0.342420481092807 0.000732275969501111 1.52331001708955 5.43111193537147e-20 MirTarget;miRNATAP -0.548144739035342 1.92986468080947e-22 NA NA NA hsa-miR-106a-5p COL2A1 0.574594373206545 0.000145868284001732 -0.251803518620361 0.565281153122427 miRNAWalker2_validate -0.275850943813436 0.00531050093299209 NA NA NA hsa-miR-134-5p COL2A1 -0.943054080774898 1.50079821035544e-12 -0.251803518620361 0.565281153122427 MirTarget -0.299474802648156 0.00706881355391252 NA NA NA hsa-miR-148a-3p COL2A1 0.706969798912638 5.52538085480485e-09 -0.251803518620361 0.565281153122427 MirTarget;miRNATAP -0.545964245062268 8.24499834281777e-06 NA NA NA hsa-miR-29a-3p COL4A1 -0.699539666833076 1.13935003139957e-13 -0.0605089402704468 0.561130598229782 miRNAWalker2_validate;miRTarBase;miRNATAP -0.171325582149256 4.35526768853979e-06 NA NA NA hsa-miR-29b-3p COL4A1 0.438847962603729 6.46722433236121e-05 -0.0605089402704468 0.561130598229782 miRNAWalker2_validate;miRTarBase;miRNATAP -0.109543469213696 0.000720046923468798 NA NA NA hsa-miR-29c-3p COL4A1 -0.0652732378039769 0.563774115756922 -0.0605089402704468 0.561130598229782 miRNAWalker2_validate;miRTarBase;miRNATAP -0.187629765989983 2.23164625787479e-09 NA NA NA hsa-miR-320a COL4A1 -0.287921276129346 0.00141693637596294 -0.0605089402704468 0.561130598229782 miRanda -0.125074040546733 0.00159999785396685 NA NA NA hsa-miR-429 COL4A1 2.84108892259147 3.88520896426515e-64 -0.0605089402704468 0.561130598229782 miRanda;miRNATAP -0.117661971659563 1.69676712956939e-09 NA NA NA hsa-miR-1266-5p COL4A2 0.413134124142464 0.00276117940829758 -0.27545094339531 0.00549451640901307 MirTarget -0.105238465598966 1.87945370415893e-05 NA NA NA hsa-miR-29b-3p COL4A2 0.438847962603729 6.46722433236121e-05 -0.27545094339531 0.00549451640901307 miRTarBase;MirTarget;miRNATAP -0.135282087031871 1.16484172805038e-05 NA NA NA hsa-miR-29c-3p COL4A2 -0.0652732378039769 0.563774115756922 -0.27545094339531 0.00549451640901307 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.147928470005093 8.61297436940001e-07 NA NA NA hsa-miR-107 COL4A3 0.678998026350662 2.56224148930538e-16 -2.06982209902333 2.89268812766655e-17 miRanda -0.290177968191992 0.00451769911312246 NA NA NA hsa-miR-193a-3p COL4A3 0.310617999804917 0.00477640270974957 -2.06982209902333 2.89268812766655e-17 miRanda -0.306065151318904 8.33617314539901e-05 NA NA NA hsa-miR-200b-3p COL4A3 2.11744072703209 1.66710572104191e-43 -2.06982209902333 2.89268812766655e-17 TargetScan -0.138361560009036 0.00908463546638511 NA NA NA hsa-miR-3065-3p COL4A3 1.62787885002468 6.81477830005026e-19 -2.06982209902333 2.89268812766655e-17 miRNATAP -0.147236046061633 0.00125702921891741 NA NA NA hsa-miR-429 COL4A3 2.84108892259147 3.88520896426515e-64 -2.06982209902333 2.89268812766655e-17 miRanda;miRNATAP -0.182052452818027 0.00011657104661419 NA NA NA hsa-miR-625-5p COL4A3 0.728939236913178 5.97591217674307e-08 -2.06982209902333 2.89268812766655e-17 MirTarget -0.240089656925081 0.000233493693423378 NA NA NA hsa-let-7a-3p COL4A4 0.466622567891891 3.70886257943079e-07 -1.19780850940918 1.1373009393889e-10 mirMAP -0.223954487806268 0.00138813790777451 NA NA NA hsa-miR-182-5p COL4A4 2.36408084103818 2.60011457600281e-72 -1.19780850940918 1.1373009393889e-10 MirTarget -0.336060797774185 2.60983267806506e-14 NA NA NA hsa-miR-30a-3p COL4A4 -1.08956435286856 1.10750674569595e-12 -1.19780850940918 1.1373009393889e-10 MirTarget -0.131144000319044 0.00156872446679793 NA NA NA hsa-miR-429 COL4A4 2.84108892259147 3.88520896426515e-64 -1.19780850940918 1.1373009393889e-10 miRNATAP -0.12335076045166 0.000521126476669951 NA NA NA hsa-let-7g-5p COL4A5 0.0200025784917734 0.791635298815256 -0.0491438803766426 0.8090167163916 miRNATAP -0.442667884296341 1.72760454704686e-06 NA NA NA hsa-let-7i-5p COL4A5 -0.333419326258177 3.56960810786801e-08 -0.0491438803766426 0.8090167163916 miRNATAP -0.450693367240488 9.99432795965474e-05 NA NA NA hsa-miR-193a-3p COL4A5 0.310617999804917 0.00477640270974957 -0.0491438803766426 0.8090167163916 miRanda -0.167233927578917 0.00834140012708126 NA NA NA hsa-miR-23a-3p COL4A5 -0.217176729033818 0.00962615056202409 -0.0491438803766426 0.8090167163916 miRNATAP -0.432872009136295 1.88233716421592e-07 NA NA NA hsa-miR-23b-3p COL4A5 -0.363202401123049 0.000265305319764878 -0.0491438803766426 0.8090167163916 miRNATAP -0.183725466501816 0.00872612672959091 NA NA NA hsa-miR-29a-3p COL4A5 -0.699539666833076 1.13935003139957e-13 -0.0491438803766426 0.8090167163916 miRNATAP -0.200342338506775 0.0061843041043234 NA NA NA hsa-miR-29b-3p COL4A5 0.438847962603729 6.46722433236121e-05 -0.0491438803766426 0.8090167163916 miRNATAP -0.370545083336625 3.7625208677457e-09 NA NA NA hsa-miR-455-5p COL4A5 0.284641224662773 0.0357572339594277 -0.0491438803766426 0.8090167163916 miRanda -0.247765611524464 1.31405874613699e-06 NA NA NA hsa-miR-590-3p COL4A5 1.27438512890514 1.03164723481469e-23 -0.0491438803766426 0.8090167163916 miRanda;mirMAP -0.387023073879309 2.29048689681589e-12 NA NA NA hsa-miR-197-3p COL4A6 -0.526589453596294 3.61594599437195e-06 -2.60404497415182 4.59834952999788e-22 miRNAWalker2_validate -0.492695160307756 2.46301393945038e-09 NA NA NA hsa-miR-29b-3p COL4A6 0.438847962603729 6.46722433236121e-05 -2.60404497415182 4.59834952999788e-22 miRNATAP -0.551409657371208 1.13195658502441e-10 NA NA NA hsa-miR-330-5p COL4A6 0.626063225953928 3.42215028606779e-09 -2.60404497415182 4.59834952999788e-22 miRanda -0.66932591825018 2.45448385494104e-14 NA NA NA hsa-miR-429 COL4A6 2.84108892259147 3.88520896426515e-64 -2.60404497415182 4.59834952999788e-22 miRanda;miRNATAP -0.301737950731445 6.80185259616497e-09 NA NA NA hsa-miR-590-3p COL4A6 1.27438512890514 1.03164723481469e-23 -2.60404497415182 4.59834952999788e-22 miRanda -0.707845679390344 1.59232097945889e-21 NA NA NA hsa-miR-96-5p COL4A6 3.24596558166606 1.92296159041707e-98 -2.60404497415182 4.59834952999788e-22 TargetScan -0.358638324372274 3.60799197577596e-11 NA NA NA hsa-miR-1301-3p COL6A1 1.80754972811286 2.96809840886499e-36 0.502660048227115 0.000176004058519763 miRNAWalker2_validate -0.160696581865755 1.35240439888712e-07 NA NA NA hsa-miR-324-5p COL6A1 0.211552647440779 0.139408130311654 0.502660048227115 0.000176004058519763 miRNAWalker2_validate -0.185508142685916 6.60612265650412e-09 NA NA NA hsa-miR-335-5p COL6A1 -2.61355235628987 2.6462884165886e-51 0.502660048227115 0.000176004058519763 miRNAWalker2_validate -0.113562051975344 4.99241838822059e-06 NA NA NA hsa-miR-29a-3p COL6A3 -0.699539666833076 1.13935003139957e-13 0.634653480531805 2.31261246968383e-05 MirTarget;miRNATAP -0.330831999684707 7.53633825609695e-10 NA NA NA hsa-miR-29b-3p COL6A3 0.438847962603729 6.46722433236121e-05 0.634653480531805 2.31261246968383e-05 MirTarget;miRNATAP -0.457250270399693 1.49496395063949e-23 NA NA NA hsa-miR-29c-3p COL6A3 -0.0652732378039769 0.563774115756922 0.634653480531805 2.31261246968383e-05 MirTarget;miRNATAP -0.288244757618725 2.13967705729232e-10 NA NA NA hsa-miR-320a COL6A3 -0.287921276129346 0.00141693637596294 0.634653480531805 2.31261246968383e-05 miRanda -0.201176748305143 0.000453248407381851 NA NA NA hsa-miR-320b COL6A3 0.412787504747938 0.00113457276903985 0.634653480531805 2.31261246968383e-05 miRanda -0.167413670186361 4.06185936806191e-05 NA NA NA hsa-miR-330-3p COL6A3 0.432728362037032 0.00041335506960812 0.634653480531805 2.31261246968383e-05 miRNATAP -0.133167329635563 0.00179688706587385 NA NA NA hsa-miR-429 COL6A3 2.84108892259147 3.88520896426515e-64 0.634653480531805 2.31261246968383e-05 miRNATAP -0.156049616870907 3.65967820567379e-08 NA NA NA hsa-miR-590-3p COL6A3 1.27438512890514 1.03164723481469e-23 0.634653480531805 2.31261246968383e-05 miRanda -0.243582975224293 2.98385710508217e-09 NA NA NA hsa-miR-375 COL6A6 2.09893673049766 1.68351780396203e-16 -4.13623144944441 2.15085613287136e-58 miRanda -0.280960121016173 5.00098926026836e-15 NA NA NA hsa-miR-429 COL6A6 2.84108892259147 3.88520896426515e-64 -4.13623144944441 2.15085613287136e-58 miRanda -0.565610558287695 5.3336432223487e-29 NA NA NA hsa-miR-590-3p COL6A6 1.27438512890514 1.03164723481469e-23 -4.13623144944441 2.15085613287136e-58 MirTarget;miRanda;mirMAP -0.509291806228668 1.21708012589005e-11 NA NA NA hsa-miR-590-5p COL6A6 0.746515607868274 8.51300479640125e-09 -4.13623144944441 2.15085613287136e-58 miRanda -0.419690416151172 5.73141327322401e-09 NA NA NA hsa-miR-335-5p COL9A2 -2.61355235628987 2.6462884165886e-51 0.765612393916573 0.000156825908929372 miRNAWalker2_validate -0.170476149910631 5.85079319606979e-06 NA NA NA hsa-miR-491-5p COL9A2 -1.04307210952796 1.14586501470398e-15 0.765612393916573 0.000156825908929372 miRanda -0.222191018163068 2.62104147516179e-05 NA NA NA hsa-miR-335-5p COL9A3 -2.61355235628987 2.6462884165886e-51 0.937179586048349 0.000732672788450644 miRNAWalker2_validate -0.285342670699414 2.74513332001203e-08 NA NA NA hsa-miR-486-5p COL9A3 -3.87258704301474 1.22658629378451e-80 0.937179586048349 0.000732672788450644 miRanda -0.109450786795404 0.00965430825486021 NA NA NA hsa-miR-103a-3p CREB1 0.610673390034345 1.63113920514646e-12 -0.391941761440804 1.10476751813217e-13 miRNAWalker2_validate -0.236524244723723 1.58919450111743e-31 NA NA NA hsa-miR-106b-5p CREB1 1.22477789913175 2.31787213143326e-32 -0.391941761440804 1.10476751813217e-13 MirTarget -0.12214492226147 4.2060764980789e-13 NA NA NA hsa-miR-181a-5p CREB1 0.635863607630183 6.72486918157744e-11 -0.391941761440804 1.10476751813217e-13 MirTarget -0.145247347990584 2.57897118284946e-15 NA NA NA hsa-miR-181b-5p CREB1 1.22749257795094 1.21998198830919e-27 -0.391941761440804 1.10476751813217e-13 MirTarget -0.13460523512347 2.85717415499498e-18 NA NA NA hsa-miR-20a-5p CREB1 0.44979911120222 0.000602386964327228 -0.391941761440804 1.10476751813217e-13 MirTarget -0.122752721271901 2.42908982512804e-19 NA NA NA hsa-miR-340-5p CREB1 1.0857159627596 4.94471062546756e-24 -0.391941761440804 1.10476751813217e-13 MirTarget -0.136655827138553 5.74841751940366e-17 NA NA NA hsa-miR-93-5p CREB1 1.01788600209314 1.24312921618126e-21 -0.391941761440804 1.10476751813217e-13 MirTarget -0.14927421972926 1.08632172077908e-19 NA NA NA hsa-miR-140-5p CREB3L1 -0.755971041589807 9.68060922934542e-14 1.98276552200667 1.75546430825192e-32 MirTarget;miRanda;miRNATAP -0.449786908758343 3.50727194185209e-15 NA NA NA hsa-miR-217 CREB3L1 -0.616728851115349 8.64977119332241e-05 1.98276552200667 1.75546430825192e-32 miRanda -0.142603436298519 0.000159936598455683 NA NA NA hsa-miR-320a CREB3L1 -0.287921276129346 0.00141693637596294 1.98276552200667 1.75546430825192e-32 miRanda -0.247469185570462 0.000186040941211091 NA NA NA hsa-miR-320b CREB3L1 0.412787504747938 0.00113457276903985 1.98276552200667 1.75546430825192e-32 miRanda -0.128073148611697 0.00671008068143745 NA NA NA hsa-let-7e-5p CREB3L2 0.261471269338985 0.00191077095550257 -0.451029929545694 1.56006948492969e-06 mirMAP -0.121467436652324 0.001643364104474 NA NA NA hsa-miR-128-3p CREB3L2 0.90296715955238 9.925204823863e-23 -0.451029929545694 1.56006948492969e-06 MirTarget -0.119426935259211 0.000508645100546543 NA NA NA hsa-miR-181a-5p CREB3L2 0.635863607630183 6.72486918157744e-11 -0.451029929545694 1.56006948492969e-06 mirMAP -0.141800694233451 1.60662132188049e-05 NA NA NA hsa-miR-181b-5p CREB3L2 1.22749257795094 1.21998198830919e-27 -0.451029929545694 1.56006948492969e-06 mirMAP -0.117451063086849 2.32388428702006e-05 NA NA NA hsa-miR-185-5p CREB3L2 0.482334746589337 3.68475879959415e-09 -0.451029929545694 1.56006948492969e-06 MirTarget -0.138872386884871 0.000430263489198159 NA NA NA hsa-miR-361-5p CREB3L2 -0.0996867547900457 0.095428250126326 -0.451029929545694 1.56006948492969e-06 miRanda -0.272213836067506 6.73098807824907e-07 NA NA NA hsa-miR-140-5p CREB3L4 -0.755971041589807 9.68060922934542e-14 1.73073054414657 1.90935037753166e-37 miRanda -0.565434412243365 1.05073680508571e-35 NA NA NA hsa-miR-106b-5p CREB5 1.22477789913175 2.31787213143326e-32 -2.34983683376521 9.5898827522032e-62 MirTarget;miRNATAP -0.308690587890586 1.98100069238304e-10 NA NA NA hsa-miR-107 CREB5 0.678998026350662 2.56224148930538e-16 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.412789211600665 2.73723888049064e-11 NA NA NA hsa-miR-130b-3p CREB5 1.38122163823846 1.00932179737836e-26 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.140662561817587 0.000385312968028588 NA NA NA hsa-miR-141-3p CREB5 2.99777540204168 1.26370498493024e-78 -2.34983683376521 9.5898827522032e-62 mirMAP -0.420728186922246 3.61586637494311e-51 NA NA NA hsa-miR-15a-5p CREB5 0.812445324157528 4.50263417099822e-20 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.461767374208094 7.7767953069944e-16 NA NA NA hsa-miR-15b-5p CREB5 0.788659356634696 1.13678211092534e-11 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.193623063328066 1.42069695080919e-05 NA NA NA hsa-miR-182-5p CREB5 2.36408084103818 2.60011457600281e-72 -2.34983683376521 9.5898827522032e-62 mirMAP -0.489369301103731 1.31250062187772e-45 NA NA NA hsa-miR-200a-3p CREB5 2.55218480823434 2.96135446816238e-51 -2.34983683376521 9.5898827522032e-62 mirMAP -0.329840324236737 9.82195063284642e-32 NA NA NA hsa-miR-200b-3p CREB5 2.11744072703209 1.66710572104191e-43 -2.34983683376521 9.5898827522032e-62 TargetScan -0.364418430782804 2.65438137279006e-31 NA NA NA hsa-miR-200c-3p CREB5 2.07038651806183 6.84613386286599e-51 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.482139236249271 2.9707359635911e-46 NA NA NA hsa-miR-21-3p CREB5 2.17476065946878 5.15299442583591e-75 -2.34983683376521 9.5898827522032e-62 mirMAP -0.269944075717791 1.31071184087826e-11 NA NA NA hsa-miR-29b-3p CREB5 0.438847962603729 6.46722433236121e-05 -2.34983683376521 9.5898827522032e-62 MirTarget;miRNATAP -0.310271402829483 5.34972288886291e-11 NA NA NA hsa-miR-29c-3p CREB5 -0.0652732378039769 0.563774115756922 -2.34983683376521 9.5898827522032e-62 MirTarget;miRNATAP -0.298729601247434 9.64097729917226e-11 NA NA NA hsa-miR-301a-3p CREB5 1.32170168375632 2.12280368957514e-15 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.142689792524629 4.39811910634395e-06 NA NA NA hsa-miR-3065-3p CREB5 1.62787885002468 6.81477830005026e-19 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.18869578587965 9.40074303771644e-12 NA NA NA hsa-miR-30d-3p CREB5 0.264544633563642 0.00755023189129747 -2.34983683376521 9.5898827522032e-62 mirMAP -0.220756284404453 3.22354624139063e-05 NA NA NA hsa-miR-32-5p CREB5 1.38676603945192 2.29428299685976e-34 -2.34983683376521 9.5898827522032e-62 miRNAWalker2_validate -0.334064316598883 2.52810063541936e-14 NA NA NA hsa-miR-330-3p CREB5 0.432728362037032 0.00041335506960812 -2.34983683376521 9.5898827522032e-62 MirTarget;mirMAP -0.138229989698104 0.00144458373630847 NA NA NA hsa-miR-340-5p CREB5 1.0857159627596 4.94471062546756e-24 -2.34983683376521 9.5898827522032e-62 mirMAP -0.285540549985062 1.57632638687561e-09 NA NA NA hsa-miR-429 CREB5 2.84108892259147 3.88520896426515e-64 -2.34983683376521 9.5898827522032e-62 PITA;miRanda;miRNATAP -0.314986461501622 2.7535498159881e-29 NA NA NA hsa-miR-454-3p CREB5 1.28177672191095 6.84024979484587e-21 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.253683640457982 1.08991816762107e-11 NA NA NA hsa-miR-590-3p CREB5 1.27438512890514 1.03164723481469e-23 -2.34983683376521 9.5898827522032e-62 PITA;mirMAP -0.170944568767965 4.70464404899778e-05 NA NA NA hsa-miR-671-5p CREB5 1.55248047544015 7.7377784125886e-33 -2.34983683376521 9.5898827522032e-62 PITA -0.291477585831714 8.2288580629852e-14 NA NA NA hsa-miR-7-1-3p CREB5 0.686579567507582 1.20731554448806e-07 -2.34983683376521 9.5898827522032e-62 mirMAP -0.205634751390653 2.79612461437901e-07 NA NA NA hsa-miR-769-5p CREB5 0.564871766265874 8.16695101656941e-10 -2.34983683376521 9.5898827522032e-62 miRNATAP -0.220681081909913 9.99620553006075e-05 NA NA NA hsa-miR-93-5p CREB5 1.01788600209314 1.24312921618126e-21 -2.34983683376521 9.5898827522032e-62 MirTarget;miRNATAP -0.311717733042225 6.46597401068875e-11 NA NA NA hsa-miR-106a-5p CSF1 0.574594373206545 0.000145868284001732 -1.08800144751585 6.47455154417147e-21 miRNATAP -0.112856920765228 2.52328970494851e-05 NA NA NA hsa-miR-106b-5p CSF1 1.22477789913175 2.31787213143326e-32 -1.08800144751585 6.47455154417147e-21 miRNATAP -0.309958906216993 9.90650672280216e-17 NA NA NA hsa-miR-107 CSF1 0.678998026350662 2.56224148930538e-16 -1.08800144751585 6.47455154417147e-21 miRanda -0.24596030061089 3.60923141846531e-07 NA NA NA hsa-miR-128-3p CSF1 0.90296715955238 9.925204823863e-23 -1.08800144751585 6.47455154417147e-21 MirTarget -0.194031179733804 6.9301013192568e-06 22909061 By mutations in putative miRNA targets in CSF-1 mRNA 3'UTR we identified a common target for both miR-128 and miR-152; We have also found that both miR-128 and miR-152 down-regulate CSF-1 mRNA and protein expression in ovarian cancer cells leading to decreased cell motility and adhesion in vitro two major aspects of the metastatic potential of cancer cells; Our results provide the evidence for a mechanism by which miR-128 and miR-152 down-regulate CSF-1 an important regulator of ovarian cancer motility ovarian cancer hsa-miR-130b-3p CSF1 1.38122163823846 1.00932179737836e-26 -1.08800144751585 6.47455154417147e-21 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.188326147995992 6.66850435979277e-10 22005523 CSF-1 regulated by miR-130b was detected using Dual Luciferase Reporter system; CSF-1 expression was negatively associated with miR-130b level in ovarian tissues and cell lines; Luciferase assay validated CSF-1 is a direct target of miR-130b; Knock-down of CSF-1 sensitized ovarian cancer cells to anticancer drugs and could partially attenuate the resistance inducing effect of miR-130b inhibitors; Downregulation of miR-130b promotes the development of multidrug resistant ovarian cancer partially by targeting the 3'-UTR of CSF-1 and the silencing of miR-130b may be mediated by DNA methylation drug resistance ovarian cancer hsa-miR-148b-3p CSF1 1.0552576751879 1.6679285705231e-29 -1.08800144751585 6.47455154417147e-21 miRNAWalker2_validate -0.187041171487231 8.71634592486979e-06 NA NA NA hsa-miR-149-5p CSF1 0.218233101703391 0.261629158371175 -1.08800144751585 6.47455154417147e-21 MirTarget -0.144048188958803 3.45004343973706e-12 NA NA NA hsa-miR-15b-5p CSF1 0.788659356634696 1.13678211092534e-11 -1.08800144751585 6.47455154417147e-21 miRNATAP -0.117803030142251 0.00068024246553631 NA NA NA hsa-miR-17-5p CSF1 0.599531757298529 1.07318660415906e-05 -1.08800144751585 6.47455154417147e-21 TargetScan;miRNATAP -0.174864005974289 3.32952325826593e-09 NA NA NA hsa-miR-20a-5p CSF1 0.44979911120222 0.000602386964327228 -1.08800144751585 6.47455154417147e-21 miRNATAP -0.169006912067568 4.22612100145374e-08 NA NA NA hsa-miR-301a-3p CSF1 1.32170168375632 2.12280368957514e-15 -1.08800144751585 6.47455154417147e-21 MirTarget;miRNATAP -0.158253418441984 3.86536901326118e-11 NA NA NA hsa-miR-429 CSF1 2.84108892259147 3.88520896426515e-64 -1.08800144751585 6.47455154417147e-21 PITA;miRanda;miRNATAP -0.19084665356121 5.34746781839834e-18 NA NA NA hsa-miR-454-3p CSF1 1.28177672191095 6.84024979484587e-21 -1.08800144751585 6.47455154417147e-21 MirTarget;miRNATAP -0.171181789004693 3.84549274234278e-09 NA NA NA hsa-miR-93-5p CSF1 1.01788600209314 1.24312921618126e-21 -1.08800144751585 6.47455154417147e-21 miRNATAP -0.306827220898973 6.48739588242599e-17 NA NA NA hsa-miR-96-5p CSF1 3.24596558166606 1.92296159041707e-98 -1.08800144751585 6.47455154417147e-21 miRNATAP -0.252185993359345 1.25546616030164e-28 NA NA NA hsa-miR-141-3p CSF3 2.99777540204168 1.26370498493024e-78 -3.88687956855714 5.79181068171002e-45 TargetScan;miRNATAP -0.635639647527127 3.84247648434209e-31 NA NA NA hsa-miR-200a-3p CSF3 2.55218480823434 2.96135446816238e-51 -3.88687956855714 5.79181068171002e-45 miRNATAP -0.476735635349525 2.71347727473529e-18 NA NA NA hsa-miR-30b-3p CSF3R -0.536462824497982 2.41814659272548e-05 0.736535697973049 2.28188447740517e-06 MirTarget -0.135966153005423 0.00133826642524163 NA NA NA hsa-miR-30a-5p DDIT4 -0.392489994825279 0.00954894667761676 0.602588460594883 3.44966750609546e-05 MirTarget;miRNATAP -0.105191972168508 0.00144674135390842 NA NA NA hsa-miR-30b-5p DDIT4 -0.46540603860146 2.4709940429591e-05 0.602588460594883 3.44966750609546e-05 MirTarget -0.122846576336623 0.00672964517682991 NA NA NA hsa-miR-218-5p EFNA1 -1.02621788318234 2.9221925445771e-23 1.03416794486437 5.98415403389838e-26 miRNAWalker2_validate -0.223154705444494 5.84928129078387e-12 NA NA NA hsa-miR-326 EFNA1 -1.54449030848141 1.04703417845594e-17 1.03416794486437 5.98415403389838e-26 miRanda -0.177390601452322 5.79220039722558e-22 NA NA NA hsa-miR-335-5p EFNA1 -2.61355235628987 2.6462884165886e-51 1.03416794486437 5.98415403389838e-26 miRNAWalker2_validate -0.128525356807881 4.72540778045499e-12 NA NA NA hsa-miR-486-5p EFNA1 -3.87258704301474 1.22658629378451e-80 1.03416794486437 5.98415403389838e-26 miRanda -0.133549219076437 7.71718683921977e-19 NA NA NA hsa-miR-491-5p EFNA1 -1.04307210952796 1.14586501470398e-15 1.03416794486437 5.98415403389838e-26 miRanda -0.113664637321473 1.57091587986049e-05 NA NA NA hsa-miR-145-5p EFNA3 -2.62703571936687 7.64402900188551e-88 1.58152606743336 5.43547499348979e-24 miRNATAP -0.225183758775436 1.30937642878182e-09 NA NA NA hsa-miR-30a-5p EFNA3 -0.392489994825279 0.00954894667761676 1.58152606743336 5.43547499348979e-24 MirTarget;mirMAP;miRNATAP -0.219092642537128 1.3512657859834e-09 NA NA NA hsa-miR-326 EFNA3 -1.54449030848141 1.04703417845594e-17 1.58152606743336 5.43547499348979e-24 MirTarget;miRanda;miRNATAP -0.147651472219488 7.90737227603787e-07 NA NA NA hsa-miR-486-5p EFNA3 -3.87258704301474 1.22658629378451e-80 1.58152606743336 5.43547499348979e-24 miRanda -0.24448323270154 9.12226705330056e-25 NA NA NA hsa-miR-140-5p EFNA4 -0.755971041589807 9.68060922934542e-14 1.36555460859862 9.20640991197574e-28 PITA;miRanda -0.167870909172277 9.74014850651836e-05 NA NA NA hsa-miR-182-5p EFNA5 2.36408084103818 2.60011457600281e-72 -1.37163072863579 4.49535560121869e-12 mirMAP;miRNATAP -0.345456013207985 2.56625510585803e-13 NA NA NA hsa-miR-200c-3p EFNA5 2.07038651806183 6.84613386286599e-51 -1.37163072863579 4.49535560121869e-12 miRNATAP -0.128934276846316 0.00592726380694451 NA NA NA hsa-miR-29c-3p EFNA5 -0.0652732378039769 0.563774115756922 -1.37163072863579 4.49535560121869e-12 miRNATAP -0.311367918739331 3.00972319771641e-07 NA NA NA hsa-miR-338-3p EFNA5 0.0799903526884966 0.575137668326679 -1.37163072863579 4.49535560121869e-12 miRanda;miRNATAP -0.249053958105886 2.18429568149124e-07 NA NA NA hsa-miR-342-3p EFNA5 1.21944481975657 6.81914373001202e-19 -1.37163072863579 4.49535560121869e-12 miRNATAP -0.345147728905934 1.06488395226997e-12 NA NA NA hsa-miR-7-1-3p EFNA5 0.686579567507582 1.20731554448806e-07 -1.37163072863579 4.49535560121869e-12 mirMAP -0.179610614381326 0.00065496480716735 NA NA NA hsa-miR-96-5p EFNA5 3.24596558166606 1.92296159041707e-98 -1.37163072863579 4.49535560121869e-12 TargetScan -0.238628836115784 1.35928421306458e-09 NA NA NA hsa-miR-590-3p EGF 1.27438512890514 1.03164723481469e-23 -1.56299913658955 5.86935289521553e-09 miRanda -0.381992230343087 2.5216093989528e-07 NA NA NA hsa-miR-629-3p EGF 1.25035919247365 3.91361135469024e-14 -1.56299913658955 5.86935289521553e-09 miRNATAP -0.198651351598521 0.000506937207678382 NA NA NA hsa-miR-107 EGFR 0.678998026350662 2.56224148930538e-16 -3.35228711557446 2.81857446737209e-45 miRanda -0.619981893208437 1.16509488926421e-09 NA NA NA hsa-miR-128-3p EGFR 0.90296715955238 9.925204823863e-23 -3.35228711557446 2.81857446737209e-45 miRNAWalker2_validate;miRTarBase -0.403877837900024 9.67805623106623e-06 NA NA NA hsa-miR-141-3p EGFR 2.99777540204168 1.26370498493024e-78 -3.35228711557446 2.81857446737209e-45 MirTarget -0.586858650420638 4.17631860777935e-36 26025929 Treatment with the EGFR inhibitor AG1478 or overexpression of miR141 blocked the activity of ERK downstream of EGFR and inhibited KLF8-depndent cell invasiveness proliferation and viability in cell culture and invasive growth and lung metastasis in nude mice metastasis breast cancer hsa-miR-141-5p EGFR 2.38411349683216 4.56310582620282e-58 -3.35228711557446 2.81857446737209e-45 MirTarget -0.540232230964423 7.07290618976338e-25 26025929 Treatment with the EGFR inhibitor AG1478 or overexpression of miR141 blocked the activity of ERK downstream of EGFR and inhibited KLF8-depndent cell invasiveness proliferation and viability in cell culture and invasive growth and lung metastasis in nude mice metastasis breast cancer hsa-miR-192-5p EGFR 1.33366224451777 9.5116159697107e-29 -3.35228711557446 2.81857446737209e-45 mirMAP -0.290600739714254 2.7733146554232e-05 NA NA NA hsa-miR-200a-3p EGFR 2.55218480823434 2.96135446816238e-51 -3.35228711557446 2.81857446737209e-45 MirTarget -0.529702964197266 2.19864859009139e-30 26184032;19671845 MicroRNA 200a Targets EGFR and c Met to Inhibit Migration Invasion and Gefitinib Resistance in Non Small Cell Lung Cancer; In this study we found that miR-200a is downregulated in NSCLC cells where it directly targets the 3'-UTR of both EGFR and c-Met mRNA; Overexpression of miR-200a in NSCLC cells significantly downregulates both EGFR and c-Met levels and severely inhibits cell migration and invasion;Protein expression and signaling pathway modulation as well as intracellular distribution of EGFR and ERRFI-1 were validated through Western blot analysis and confocal microscopy whereas ERRFI-1 direct target of miR-200 members was validated by using the wild-type and mutant 3'-untranslated region/ERRFI-1/luciferse reporters; We identified a tight association between the expression of miRNAs of the miR-200 family epithelial phenotype and sensitivity to EGFR inhibitors-induced growth inhibition in bladder carcinoma cell lines; The changes in EGFR sensitivity by silencing or forced expression of ERRFI-1 or by miR-200 expression have also been validated in additional cell lines UMUC5 and T24; Members of the miR-200 family appear to control the EMT process and sensitivity to EGFR therapy in bladder cancer cells and the expression of miR-200 is sufficient to restore EGFR dependency at least in some of the mesenchymal bladder cancer cells drug resistance;cell migration; lung squamous cell cancer;bladder cancer hsa-miR-21-5p EGFR 2.32007501846389 3.08394781855554e-137 -3.35228711557446 2.81857446737209e-45 miRNAWalker2_validate;miRTarBase -0.671030468978973 2.34536849626451e-18 24198203;20113523;24012640;24331411;26563758;19597153;26026961;20048743 In radically resected NSCLC patients the expression levels of miR-21 10b in patients with EGFR mutation were much higher than those without mutation;Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;Further the expression of miR-21 is regulated by EGFR via the activation of β-catenin and AP-1; These data indicate that a feedback loop exists between miR-21 and EGFR; These results clarify a novel association between miR-21 and EGFR in the regulation of cancer cell progression;MiR 21 overexpression is associated with acquired resistance of EGFR TKI in non small cell lung cancer;Higher expression levels of miR-21 AmiR-27a and miR-218 detected in this study suggest potential roles of these miRNAs in primary resistance to EGFR-TKI in advanced NSCLC patients with EGFR exon 19 deletion mutations;MiR 21 is an EGFR regulated anti apoptotic factor in lung cancer in never smokers; The changes in expression of some of these miRNAs including miR-21 were more remarkable in cases with EGFR mutations than in those without these mutations; In the never-smoker-derived lung adenocarcinoma cell line H3255 with mutant EGFR and high levels of p-EGFR and miR-21 antisense inhibition of miR-21 enhanced AG1478-induced apoptosis; In a never-smoker-derived adenocarcinoma cell line H441 with wild-type EGFR the antisense miR-21 not only showed the additive effect with AG1478 but also induced apoptosis by itself; These results suggest that aberrantly increased expression of miR-21 which is enhanced further by the activated EGFR signaling pathway plays a significant role in lung carcinogenesis in never-smokers as well as in smokers and is a potential therapeutic target in both EGFR-mutant and wild-type cases;Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR mutated lung cancer via nickel induced microRNA 21 expression;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status ;;progression;drug resistance;staging;drug resistance;tumorigenesis;; lung squamous cell cancer;glioblastoma;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;lung cancer;lung cancer;glioblastoma hsa-miR-29b-2-5p EGFR -0.263499971180298 0.0400001755611965 -3.35228711557446 2.81857446737209e-45 mirMAP -0.64036074154032 1.49079407610376e-22 NA NA NA hsa-miR-30a-5p EGFR -0.392489994825279 0.00954894667761676 -3.35228711557446 2.81857446737209e-45 miRNAWalker2_validate -0.182034532010776 0.0013553693971936 NA NA NA hsa-miR-340-5p EGFR 1.0857159627596 4.94471062546756e-24 -3.35228711557446 2.81857446737209e-45 mirMAP -0.347908635889104 8.12198670456393e-06 NA NA NA hsa-miR-342-3p EGFR 1.21944481975657 6.81914373001202e-19 -3.35228711557446 2.81857446737209e-45 miRanda -0.737335841516313 4.52090989396721e-36 NA NA NA hsa-miR-375 EGFR 2.09893673049766 1.68351780396203e-16 -3.35228711557446 2.81857446737209e-45 miRanda -0.424868499428666 2.03176717536657e-41 NA NA NA hsa-miR-3913-5p EGFR 0.71890413546147 2.61153227160465e-13 -3.35228711557446 2.81857446737209e-45 mirMAP -0.573576131982504 2.54015647019285e-11 NA NA NA hsa-miR-590-3p EGFR 1.27438512890514 1.03164723481469e-23 -3.35228711557446 2.81857446737209e-45 mirMAP -0.178661531450736 0.00972280347923564 NA NA NA hsa-miR-7-1-3p EGFR 0.686579567507582 1.20731554448806e-07 -3.35228711557446 2.81857446737209e-45 mirMAP -0.355654663689424 5.93286075856605e-08 NA NA NA hsa-miR-769-5p EGFR 0.564871766265874 8.16695101656941e-10 -3.35228711557446 2.81857446737209e-45 mirMAP -0.281258757298563 0.00254804504969195 NA NA NA hsa-miR-103a-3p EIF4B 0.610673390034345 1.63113920514646e-12 -0.65019836780356 1.52144443982599e-25 MirTarget;miRNATAP -0.12743739110467 3.76381682109117e-07 NA NA NA hsa-miR-107 EIF4B 0.678998026350662 2.56224148930538e-16 -0.65019836780356 1.52144443982599e-25 MirTarget;PITA;miRanda;miRNATAP -0.19588650676029 3.23415780318493e-14 NA NA NA hsa-miR-15a-5p EIF4B 0.812445324157528 4.50263417099822e-20 -0.65019836780356 1.52144443982599e-25 miRNATAP -0.158748562997593 4.58730473878287e-11 NA NA NA hsa-miR-185-5p EIF4B 0.482334746589337 3.68475879959415e-09 -0.65019836780356 1.52144443982599e-25 MirTarget -0.260058202476035 2.1915241574969e-23 NA NA NA hsa-miR-330-5p EIF4B 0.626063225953928 3.42215028606779e-09 -0.65019836780356 1.52144443982599e-25 miRNATAP -0.102458867136363 5.8358886607923e-07 NA NA NA hsa-miR-590-3p EIF4B 1.27438512890514 1.03164723481469e-23 -0.65019836780356 1.52144443982599e-25 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.157461933655438 5.99446351789919e-20 NA NA NA hsa-miR-26a-5p EIF4E -0.481180084102235 1.82368246174758e-10 0.269255020427265 1.05790226775164e-05 mirMAP -0.154217079131863 2.29252125869775e-08 NA NA NA hsa-miR-29a-3p EIF4E -0.699539666833076 1.13935003139957e-13 0.269255020427265 1.05790226775164e-05 mirMAP -0.124296545562299 1.39722119063186e-08 NA NA NA hsa-miR-361-5p EIF4E -0.0996867547900457 0.095428250126326 0.269255020427265 1.05790226775164e-05 miRanda;mirMAP -0.125999677820868 0.000415655162966611 NA NA NA hsa-miR-381-3p EIF4E -1.28028930293369 1.61402949866141e-23 0.269255020427265 1.05790226775164e-05 MirTarget;mirMAP -0.120308040349857 2.10719446650024e-14 NA NA NA hsa-miR-660-5p EIF4E 0.406910761707485 0.000156175371762464 0.269255020427265 1.05790226775164e-05 mirMAP -0.105699457757742 5.03122785788093e-08 NA NA NA hsa-miR-125a-5p EIF4EBP1 -0.635694700253268 3.6703235721638e-11 1.01321592334217 1.31060874763381e-12 miRNAWalker2_validate;MirTarget;miRanda;miRNATAP -0.262246438021565 2.76009521537076e-07 26646586 Increased expression of miR-125a and miR-125b inhibited invasion and migration of SKOV3 and OVCAR-429 ovarian cancer cells and was associated with a decrease in EIF4EBP1 expression ovarian cancer hsa-miR-125b-5p EIF4EBP1 -1.94393269763542 2.78180543229264e-58 1.01321592334217 1.31060874763381e-12 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.288352223423938 2.0288081394147e-14 17891175;26646586 Using a bioinformatics approach we have identified additional potential mRNA targets of one of the miRNAs miR-125b that are upregulated in prostate cancer and confirmed increased expression of one of these targets EIF4EBP1 in prostate cancer tissues;Increased expression of miR-125a and miR-125b inhibited invasion and migration of SKOV3 and OVCAR-429 ovarian cancer cells and was associated with a decrease in EIF4EBP1 expression ; prostate cancer;ovarian cancer hsa-miR-874-3p EIF4EBP1 -0.513415443180424 1.49285755109414e-06 1.01321592334217 1.31060874763381e-12 MirTarget -0.161830800923173 0.000481724419110573 NA NA NA hsa-miR-141-3p EPHA2 2.99777540204168 1.26370498493024e-78 -1.25921912819417 1.34618358519816e-29 MirTarget;TargetScan;miRNATAP -0.184299260927489 6.45342555838155e-17 24647573 miR 141 is a key regulator of renal cell carcinoma proliferation and metastasis by controlling EphA2 expression; In addition a specific and inverse correlation between miR-141 and EphA2 expression was obtained in human ccRCC samples metastasis kidney renal cell cancer hsa-miR-16-5p EPHA2 0.46835991343556 1.91125356547776e-06 -1.25921912819417 1.34618358519816e-29 miRNAWalker2_validate -0.176298379806946 1.09345046915033e-05 NA NA NA hsa-miR-193b-3p EPHA2 -0.0550374855074773 0.65382485898551 -1.25921912819417 1.34618358519816e-29 miRNAWalker2_validate -0.137802930852288 1.94517184204738e-05 NA NA NA hsa-miR-200a-3p EPHA2 2.55218480823434 2.96135446816238e-51 -1.25921912819417 1.34618358519816e-29 MirTarget;miRNATAP -0.119330959159105 5.02146466331342e-08 22562546;26088362;24503464 Our studies propose EphA2 as a novel and important target gene for miR-200a;miR 200a inhibits migration of triple negative breast cancer cells through direct repression of the EPHA2 oncogene; We show that EPHA2 expression is correlated with poor survival specifically in basal-like breast cancer and that its expression is repressed by miR-200a through direct interaction with the 3'UTR of EPHA2; We establish that miR-200a directs cell migration in a dual manner; in addition to regulating the well-characterized E-cadherin pathway it also regulates a EPHA2 pathway;In addition our results suggested that miR-200a inhibited VM by negatively regulated EphA2 expression; Consistently the inverse correlation of miR-200a and EphA2 has also been found in ovarian cancer patients; Our research demonstrates that VM miR-200a and EphA2 play key roles in the progression and prognosis of ovarian cancer and for the first time suggests that miR-200a inhibits VM by directly regulating EphA2 ;poor survival;cell migration;progression;worse prognosis breast cancer;breast cancer;ovarian cancer hsa-miR-326 EPO -1.54449030848141 1.04703417845594e-17 1.40724311276675 1.13464399997793e-07 miRanda -0.181890696370513 0.000257743171960766 NA NA NA hsa-miR-16-2-3p F2R 0.286355254959005 0.0256946046884474 0.702219039843029 8.69085413326007e-10 mirMAP -0.254347124696681 8.59672725339529e-17 NA NA NA hsa-miR-20a-3p F2R -0.218902047543766 0.179868363625547 0.702219039843029 8.69085413326007e-10 MirTarget -0.17506207216097 3.4102708945352e-13 NA NA NA hsa-miR-29a-5p F2R 0.334183773952055 0.00445137265870197 0.702219039843029 8.69085413326007e-10 MirTarget -0.162852891661445 1.35622791230877e-06 NA NA NA hsa-miR-361-5p F2R -0.0996867547900457 0.095428250126326 0.702219039843029 8.69085413326007e-10 miRanda -0.377684065174951 1.71825126165987e-08 NA NA NA hsa-miR-590-3p F2R 1.27438512890514 1.03164723481469e-23 0.702219039843029 8.69085413326007e-10 miRanda;mirMAP;miRNATAP -0.138556676650043 1.22092606633209e-05 NA NA NA hsa-miR-590-5p F2R 0.746515607868274 8.51300479640125e-09 0.702219039843029 8.69085413326007e-10 miRanda -0.19133954143666 2.01430047713439e-10 NA NA NA hsa-miR-664a-3p F2R -0.237699857923843 0.0197071390183375 0.702219039843029 8.69085413326007e-10 mirMAP -0.19897073658207 2.95044882103516e-07 NA NA NA hsa-let-7a-5p FASLG -0.210286642041636 0.0072546234707717 0.202786291597416 0.303256769370056 TargetScan;miRNATAP -0.254557236142898 0.0033640838487851 NA NA NA hsa-let-7b-5p FASLG -0.54215483448257 7.21347617597858e-08 0.202786291597416 0.303256769370056 miRNATAP -0.244673453612857 0.000244706869361269 NA NA NA hsa-let-7e-5p FASLG 0.261471269338985 0.00191077095550257 0.202786291597416 0.303256769370056 miRNATAP -0.337100053640702 2.69864596660333e-05 NA NA NA hsa-miR-16-1-3p FGF1 1.30142784811196 2.53343713753744e-29 -2.3363200609921 2.42261900591497e-58 mirMAP -0.581904531746624 8.04220781640102e-38 NA NA NA hsa-miR-186-5p FGF1 -0.426987301460247 1.04129380549315e-05 -2.3363200609921 2.42261900591497e-58 mirMAP -0.143723594837616 0.00933754138773196 NA NA NA hsa-miR-18a-5p FGF1 0.969066793715868 1.69069796425892e-08 -2.3363200609921 2.42261900591497e-58 MirTarget -0.308725947141596 9.70167258927243e-25 NA NA NA hsa-miR-193a-3p FGF1 0.310617999804917 0.00477640270974957 -2.3363200609921 2.42261900591497e-58 PITA -0.19690022881916 5.07868444768763e-05 NA NA NA hsa-miR-21-5p FGF1 2.32007501846389 3.08394781855554e-137 -2.3363200609921 2.42261900591497e-58 MirTarget -0.371477796734357 9.2806521720649e-15 NA NA NA hsa-miR-29a-5p FGF1 0.334183773952055 0.00445137265870197 -2.3363200609921 2.42261900591497e-58 mirMAP -0.152220555461099 0.000862510868875038 NA NA NA hsa-miR-320b FGF1 0.412787504747938 0.00113457276903985 -2.3363200609921 2.42261900591497e-58 miRanda -0.230047658868655 4.79239651855213e-08 NA NA NA hsa-miR-330-5p FGF1 0.626063225953928 3.42215028606779e-09 -2.3363200609921 2.42261900591497e-58 miRanda;miRNATAP -0.320213335163115 1.22351851936422e-10 NA NA NA hsa-miR-361-5p FGF1 -0.0996867547900457 0.095428250126326 -2.3363200609921 2.42261900591497e-58 miRanda -0.324186160458905 0.000365896398010837 NA NA NA hsa-miR-3913-5p FGF1 0.71890413546147 2.61153227160465e-13 -2.3363200609921 2.42261900591497e-58 mirMAP -0.147478952504203 0.00632555140399931 NA NA NA hsa-miR-429 FGF1 2.84108892259147 3.88520896426515e-64 -2.3363200609921 2.42261900591497e-58 miRNATAP -0.325212393355327 4.60753740095485e-30 NA NA NA hsa-miR-484 FGF1 0.349444060004737 0.00154491249625173 -2.3363200609921 2.42261900591497e-58 MirTarget;PITA;miRNATAP -0.350837387269644 2.42230585979755e-13 NA NA NA hsa-miR-532-3p FGF1 -0.395473145042459 0.00686714288452761 -2.3363200609921 2.42261900591497e-58 miRNATAP -0.135400728049064 0.000208318514603032 NA NA NA hsa-miR-550a-5p FGF1 -0.12364998419382 0.410467966112492 -2.3363200609921 2.42261900591497e-58 MirTarget -0.124015992653408 0.000650072833531158 NA NA NA hsa-miR-576-5p FGF1 0.0649387707567879 0.588791966096287 -2.3363200609921 2.42261900591497e-58 PITA;mirMAP -0.195551405525261 1.13745464101402e-05 NA NA NA hsa-miR-590-5p FGF1 0.746515607868274 8.51300479640125e-09 -2.3363200609921 2.42261900591497e-58 MirTarget;miRanda;miRNATAP -0.370669749508457 2.36068553671594e-20 NA NA NA hsa-miR-625-5p FGF1 0.728939236913178 5.97591217674307e-08 -2.3363200609921 2.42261900591497e-58 MirTarget -0.241254403404484 2.5870605302491e-09 NA NA NA hsa-miR-629-5p FGF1 0.42411327890895 2.46262300605706e-05 -2.3363200609921 2.42261900591497e-58 MirTarget -0.28293563961806 8.96106894697866e-08 NA NA NA hsa-miR-200b-3p FGF10 2.11744072703209 1.66710572104191e-43 -3.32771291998068 2.19492306477012e-23 TargetScan -0.477562798964516 3.55044319062017e-11 NA NA NA hsa-let-7a-5p FGF11 -0.210286642041636 0.0072546234707717 -0.654203699022933 6.56875051109305e-06 TargetScan;mirMAP;miRNATAP -0.267012754520769 3.15077825833402e-05 NA NA NA hsa-let-7b-5p FGF11 -0.54215483448257 7.21347617597858e-08 -0.654203699022933 6.56875051109305e-06 mirMAP;miRNATAP -0.138105904475298 0.00527683385622591 NA NA NA hsa-miR-331-3p FGF11 0.74441480542886 1.17071235477799e-07 -0.654203699022933 6.56875051109305e-06 miRNATAP -0.11808385160736 0.000829591224831171 NA NA NA hsa-miR-142-5p FGF12 1.16509303143593 1.96641162209073e-13 -0.643446523257955 0.000892623765099953 miRNATAP -0.125050416843925 0.00249966609947065 NA NA NA hsa-miR-17-5p FGF12 0.599531757298529 1.07318660415906e-05 -0.643446523257955 0.000892623765099953 TargetScan -0.150807716699532 0.00194136781001414 NA NA NA hsa-miR-200b-3p FGF12 2.11744072703209 1.66710572104191e-43 -0.643446523257955 0.000892623765099953 TargetScan -0.165329959072644 5.71604844896826e-05 NA NA NA hsa-miR-429 FGF12 2.84108892259147 3.88520896426515e-64 -0.643446523257955 0.000892623765099953 miRNATAP -0.122602237479307 0.000847169715536382 NA NA NA hsa-miR-455-5p FGF12 0.284641224662773 0.0357572339594277 -0.643446523257955 0.000892623765099953 miRanda -0.362285996207897 7.81172946390217e-14 NA NA NA hsa-miR-542-3p FGF12 0.568822103820889 8.5699075257651e-07 -0.643446523257955 0.000892623765099953 miRanda -0.186594268922009 0.00113571395430377 NA NA NA hsa-miR-141-3p FGF13 2.99777540204168 1.26370498493024e-78 -0.873614793965912 3.10323995957847e-07 TargetScan -0.151235108253828 5.55097538879746e-06 NA NA NA hsa-miR-142-5p FGF13 1.16509303143593 1.96641162209073e-13 -0.873614793965912 3.10323995957847e-07 PITA -0.171463579066746 2.60043790328601e-06 NA NA NA hsa-miR-200a-5p FGF13 2.58844508672328 1.79650654234569e-56 -0.873614793965912 3.10323995957847e-07 MirTarget;miRNATAP -0.144569347995968 1.56153098119586e-05 NA NA NA hsa-miR-590-3p FGF13 1.27438512890514 1.03164723481469e-23 -0.873614793965912 3.10323995957847e-07 miRanda -0.139748708534672 0.00308905149188458 NA NA NA hsa-miR-590-3p FGF14 1.27438512890514 1.03164723481469e-23 -0.743601748556524 2.04913944157729e-05 miRanda -0.437013841225084 1.73317372660872e-20 NA NA NA hsa-miR-96-5p FGF14 3.24596558166606 1.92296159041707e-98 -0.743601748556524 2.04913944157729e-05 TargetScan -0.186868577790991 5.83679167463715e-08 NA NA NA hsa-miR-15b-5p FGF18 0.788659356634696 1.13678211092534e-11 0.356025539172725 0.0347430848266563 miRNATAP -0.278632009163133 1.22698938766975e-08 NA NA NA hsa-miR-16-5p FGF18 0.46835991343556 1.91125356547776e-06 0.356025539172725 0.0347430848266563 miRNATAP -0.407440682812312 2.18411630304885e-12 NA NA NA hsa-miR-200b-3p FGF18 2.11744072703209 1.66710572104191e-43 0.356025539172725 0.0347430848266563 TargetScan -0.225608292610904 1.9483944408265e-10 NA NA NA hsa-miR-200c-3p FGF18 2.07038651806183 6.84613386286599e-51 0.356025539172725 0.0347430848266563 miRNATAP -0.297918294437242 1.5236208240269e-14 NA NA NA hsa-miR-326 FGF18 -1.54449030848141 1.04703417845594e-17 0.356025539172725 0.0347430848266563 PITA;miRanda;miRNATAP -0.120554064884584 0.000122916185329087 NA NA NA hsa-miR-330-5p FGF18 0.626063225953928 3.42215028606779e-09 0.356025539172725 0.0347430848266563 PITA;miRanda;miRNATAP -0.264450945601235 9.7958776270154e-07 NA NA NA hsa-miR-362-5p FGF18 -0.248676340901797 0.0726089851456282 0.356025539172725 0.0347430848266563 miRNATAP -0.160150519329221 0.000123017502928388 NA NA NA hsa-miR-424-5p FGF18 0.420789584199235 0.00480101660479489 0.356025539172725 0.0347430848266563 miRNATAP -0.118258719392065 0.00224759503637084 NA NA NA hsa-miR-429 FGF18 2.84108892259147 3.88520896426515e-64 0.356025539172725 0.0347430848266563 miRNATAP -0.192282799804847 1.28904804259296e-09 NA NA NA hsa-miR-505-3p FGF18 -0.530462599544864 7.71645736541597e-05 0.356025539172725 0.0347430848266563 miRNATAP -0.37019252141977 1.51921477550048e-18 NA NA NA hsa-miR-590-5p FGF18 0.746515607868274 8.51300479640125e-09 0.356025539172725 0.0347430848266563 miRanda;miRNATAP -0.439643988772613 1.86198557821583e-24 NA NA NA hsa-miR-9-5p FGF18 0.734772328078424 0.00261982843636383 0.356025539172725 0.0347430848266563 miRNATAP -0.200718216328244 4.25037312625656e-18 NA NA NA hsa-let-7a-3p FGF2 0.466622567891891 3.70886257943079e-07 -3.62110997961421 1.11252046868458e-69 mirMAP -0.291818855316242 0.000475924803881402 NA NA NA hsa-let-7f-1-3p FGF2 0.37532726250321 0.00156021688753682 -3.62110997961421 1.11252046868458e-69 mirMAP -0.20077854516594 0.00221318919252559 NA NA NA hsa-miR-103a-3p FGF2 0.610673390034345 1.63113920514646e-12 -3.62110997961421 1.11252046868458e-69 miRNAWalker2_validate;MirTarget;miRNATAP -0.723924551136195 6.60361690701997e-17 NA NA NA hsa-miR-107 FGF2 0.678998026350662 2.56224148930538e-16 -3.62110997961421 1.11252046868458e-69 MirTarget;PITA;miRanda;miRNATAP -0.796465331145451 7.23286562379616e-19 NA NA NA hsa-miR-142-3p FGF2 1.53951237846104 2.06769936282034e-17 -3.62110997961421 1.11252046868458e-69 miRanda -0.17283302588329 2.98031372863511e-05 NA NA NA hsa-miR-155-5p FGF2 1.09766314984151 4.55650930885814e-15 -3.62110997961421 1.11252046868458e-69 miRNAWalker2_validate;mirMAP -0.14901722637598 0.00579079439331683 NA NA NA hsa-miR-15a-5p FGF2 0.812445324157528 4.50263417099822e-20 -3.62110997961421 1.11252046868458e-69 MirTarget;miRNATAP -0.693907136905669 1.18654679034038e-16 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-3p FGF2 1.12898579025388 1.18050912917317e-14 -3.62110997961421 1.11252046868458e-69 mirMAP -0.416639852881488 2.41684465169063e-16 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-5p FGF2 0.788659356634696 1.13678211092534e-11 -3.62110997961421 1.11252046868458e-69 MirTarget;miRNATAP -0.416542064799323 1.32439928134663e-10 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-16-1-3p FGF2 1.30142784811196 2.53343713753744e-29 -3.62110997961421 1.11252046868458e-69 mirMAP -0.618895974599859 1.26519964172122e-20 NA NA NA hsa-miR-16-2-3p FGF2 0.286355254959005 0.0256946046884474 -3.62110997961421 1.11252046868458e-69 mirMAP -0.190718116559883 0.00155781442617599 NA NA NA hsa-miR-16-5p FGF2 0.46835991343556 1.91125356547776e-06 -3.62110997961421 1.11252046868458e-69 miRNAWalker2_validate;MirTarget;miRNATAP -0.334764026766057 1.68868207410758e-05 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-181a-5p FGF2 0.635863607630183 6.72486918157744e-11 -3.62110997961421 1.11252046868458e-69 mirMAP -0.445820481795785 9.84329484201549e-09 NA NA NA hsa-miR-181b-5p FGF2 1.22749257795094 1.21998198830919e-27 -3.62110997961421 1.11252046868458e-69 mirMAP -0.542719617785167 6.40042754935602e-17 NA NA NA hsa-miR-181c-5p FGF2 0.574847839298605 4.51287502066478e-07 -3.62110997961421 1.11252046868458e-69 mirMAP -0.226227363468224 0.00075162633624824 NA NA NA hsa-miR-192-5p FGF2 1.33366224451777 9.5116159697107e-29 -3.62110997961421 1.11252046868458e-69 miRNAWalker2_validate -0.492571390734632 6.64406170620692e-16 NA NA NA hsa-miR-19b-1-5p FGF2 0.647704962003062 3.07687667945597e-07 -3.62110997961421 1.11252046868458e-69 mirMAP -0.220669191860133 0.000256708213140997 NA NA NA hsa-miR-200b-3p FGF2 2.11744072703209 1.66710572104191e-43 -3.62110997961421 1.11252046868458e-69 mirMAP -0.474105291344653 1.09246839719196e-24 NA NA NA hsa-miR-200c-3p FGF2 2.07038651806183 6.84613386286599e-51 -3.62110997961421 1.11252046868458e-69 mirMAP -0.615252170517975 1.40903756446366e-34 NA NA NA hsa-miR-21-3p FGF2 2.17476065946878 5.15299442583591e-75 -3.62110997961421 1.11252046868458e-69 mirMAP -0.712905950790447 1.55737305852276e-36 NA NA NA hsa-miR-21-5p FGF2 2.32007501846389 3.08394781855554e-137 -3.62110997961421 1.11252046868458e-69 mirMAP -1.0007316696677 9.45001605861665e-53 NA NA NA hsa-miR-32-5p FGF2 1.38676603945192 2.29428299685976e-34 -3.62110997961421 1.11252046868458e-69 miRNAWalker2_validate -0.601478209606274 2.96886796474821e-21 NA NA NA hsa-miR-330-3p FGF2 0.432728362037032 0.00041335506960812 -3.62110997961421 1.11252046868458e-69 mirMAP -0.190108192654775 0.00276026518304351 NA NA NA hsa-miR-429 FGF2 2.84108892259147 3.88520896426515e-64 -3.62110997961421 1.11252046868458e-69 mirMAP -0.50658475327409 1.36690059845309e-35 NA NA NA hsa-miR-503-5p FGF2 1.89861508626371 8.64468509978193e-28 -3.62110997961421 1.11252046868458e-69 miRNAWalker2_validate -0.318786957596351 2.28520886786229e-13 NA NA NA hsa-miR-590-3p FGF2 1.27438512890514 1.03164723481469e-23 -3.62110997961421 1.11252046868458e-69 miRanda;mirMAP -0.434908422832851 8.74942691621125e-13 NA NA NA hsa-miR-590-5p FGF2 0.746515607868274 8.51300479640125e-09 -3.62110997961421 1.11252046868458e-69 miRanda;mirMAP -0.340101326786598 5.86637073853323e-09 NA NA NA hsa-miR-93-3p FGF2 0.627759242044891 3.62527588304617e-06 -3.62110997961421 1.11252046868458e-69 mirMAP -0.374363069604572 2.04134280501924e-11 NA NA NA hsa-let-7a-3p FGF7 0.466622567891891 3.70886257943079e-07 -2.04584639946267 6.89325129361849e-43 MirTarget;miRNATAP -0.291554234913666 5.86497309861538e-07 NA NA NA hsa-miR-107 FGF7 0.678998026350662 2.56224148930538e-16 -2.04584639946267 6.89325129361849e-43 PITA;miRanda;miRNATAP -0.546294975137966 4.4731046654949e-18 NA NA NA hsa-miR-15a-5p FGF7 0.812445324157528 4.50263417099822e-20 -2.04584639946267 6.89325129361849e-43 miRTarBase;miRNATAP -0.531335245933583 1.07978052597967e-19 NA NA NA hsa-miR-15b-5p FGF7 0.788659356634696 1.13678211092534e-11 -2.04584639946267 6.89325129361849e-43 miRNATAP -0.294334950139095 9.5895878173005e-11 NA NA NA hsa-miR-16-1-3p FGF7 1.30142784811196 2.53343713753744e-29 -2.04584639946267 6.89325129361849e-43 mirMAP -0.437128601027858 6.61913037421969e-21 NA NA NA hsa-miR-16-2-3p FGF7 0.286355254959005 0.0256946046884474 -2.04584639946267 6.89325129361849e-43 mirMAP -0.225774074048836 7.90334438903079e-08 NA NA NA hsa-miR-16-5p FGF7 0.46835991343556 1.91125356547776e-06 -2.04584639946267 6.89325129361849e-43 miRNATAP -0.294993672554423 5.86741065196244e-08 NA NA NA hsa-miR-20a-5p FGF7 0.44979911120222 0.000602386964327228 -2.04584639946267 6.89325129361849e-43 miRNAWalker2_validate -0.243033729024757 2.41787962262626e-09 NA NA NA hsa-miR-30d-3p FGF7 0.264544633563642 0.00755023189129747 -2.04584639946267 6.89325129361849e-43 miRNATAP -0.316263621956414 5.48336610985585e-09 NA NA NA hsa-miR-320b FGF7 0.412787504747938 0.00113457276903985 -2.04584639946267 6.89325129361849e-43 miRanda -0.139459566850431 0.00108196556023217 NA NA NA hsa-miR-340-5p FGF7 1.0857159627596 4.94471062546756e-24 -2.04584639946267 6.89325129361849e-43 mirMAP -0.279133011350609 9.24446622871117e-09 NA NA NA hsa-miR-429 FGF7 2.84108892259147 3.88520896426515e-64 -2.04584639946267 6.89325129361849e-43 miRNATAP -0.366295181453287 6.14104708947163e-38 NA NA NA hsa-miR-484 FGF7 0.349444060004737 0.00154491249625173 -2.04584639946267 6.89325129361849e-43 PITA -0.168542958718501 0.000557416327492428 NA NA NA hsa-miR-590-3p FGF7 1.27438512890514 1.03164723481469e-23 -2.04584639946267 6.89325129361849e-43 PITA;miRanda;mirMAP;miRNATAP -0.299954971963049 2.12582339280064e-12 NA NA NA hsa-miR-590-5p FGF7 0.746515607868274 8.51300479640125e-09 -2.04584639946267 6.89325129361849e-43 PITA;miRanda -0.248349796532555 1.32069527646423e-09 NA NA NA hsa-miR-7-1-3p FGF7 0.686579567507582 1.20731554448806e-07 -2.04584639946267 6.89325129361849e-43 mirMAP -0.291915571429539 8.2244137316862e-13 NA NA NA hsa-miR-15a-5p FGF9 0.812445324157528 4.50263417099822e-20 -2.4852262043005 1.90052930236681e-24 miRNATAP -0.453532492537739 1.81189938850602e-06 NA NA NA hsa-miR-16-5p FGF9 0.46835991343556 1.91125356547776e-06 -2.4852262043005 1.90052930236681e-24 miRNATAP -0.265584515990919 0.00235373908707608 NA NA NA hsa-miR-181b-5p FGF9 1.22749257795094 1.21998198830919e-27 -2.4852262043005 1.90052930236681e-24 mirMAP -0.251706769659496 0.000662639171300449 NA NA NA hsa-miR-182-5p FGF9 2.36408084103818 2.60011457600281e-72 -2.4852262043005 1.90052930236681e-24 miRTarBase;miRNATAP -0.452581728187924 1.53870203091154e-14 NA NA NA hsa-miR-183-5p FGF9 2.95681814393872 1.09965777344285e-92 -2.4852262043005 1.90052930236681e-24 miRNATAP -0.421051105687966 5.55191507820402e-16 NA NA NA hsa-miR-429 FGF9 2.84108892259147 3.88520896426515e-64 -2.4852262043005 1.90052930236681e-24 miRanda -0.319482141660943 1.05794965240276e-11 NA NA NA hsa-miR-590-3p FGF9 1.27438512890514 1.03164723481469e-23 -2.4852262043005 1.90052930236681e-24 miRanda;mirMAP -0.208237924178229 0.00252074976910256 NA NA NA hsa-miR-96-5p FGF9 3.24596558166606 1.92296159041707e-98 -2.4852262043005 1.90052930236681e-24 TargetScan;miRNATAP -0.44484666162403 4.74837998245841e-20 NA NA NA hsa-miR-106a-5p FGFR1 0.574594373206545 0.000145868284001732 -1.14663914385064 6.08915041092458e-18 mirMAP -0.140764850201839 4.05395479162201e-06 NA NA NA hsa-miR-106b-5p FGFR1 1.22477789913175 2.31787213143326e-32 -1.14663914385064 6.08915041092458e-18 mirMAP -0.187712904592992 1.40602847883516e-05 NA NA NA hsa-miR-15a-5p FGFR1 0.812445324157528 4.50263417099822e-20 -1.14663914385064 6.08915041092458e-18 miRNATAP -0.158299254797074 0.00214218396058389 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-5p FGFR1 0.788659356634696 1.13678211092534e-11 -1.14663914385064 6.08915041092458e-18 miRNATAP -0.1205608912803 0.00233144736273568 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-16-5p FGFR1 0.46835991343556 1.91125356547776e-06 -1.14663914385064 6.08915041092458e-18 miRTarBase;miRNATAP -0.183714075820348 9.67034011184e-05 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-200a-5p FGFR1 2.58844508672328 1.79650654234569e-56 -1.14663914385064 6.08915041092458e-18 mirMAP -0.215721842068481 1.13267932151434e-16 NA NA NA hsa-miR-200b-5p FGFR1 1.43091697052145 2.07975242866479e-22 -1.14663914385064 6.08915041092458e-18 mirMAP -0.16607698201377 1.28945935874685e-07 NA NA NA hsa-miR-20b-5p FGFR1 0.78236583455125 8.7938334801223e-05 -1.14663914385064 6.08915041092458e-18 mirMAP -0.115810849796481 5.3473776263928e-07 NA NA NA hsa-miR-301a-3p FGFR1 1.32170168375632 2.12280368957514e-15 -1.14663914385064 6.08915041092458e-18 mirMAP -0.10939487939016 7.18051162231598e-05 NA NA NA hsa-miR-330-3p FGFR1 0.432728362037032 0.00041335506960812 -1.14663914385064 6.08915041092458e-18 PITA;miRNATAP -0.10383346919971 0.00691823598065563 NA NA NA hsa-miR-330-5p FGFR1 0.626063225953928 3.42215028606779e-09 -1.14663914385064 6.08915041092458e-18 miRanda;miRNATAP -0.200093067000453 4.15731266944936e-06 NA NA NA hsa-miR-455-5p FGFR1 0.284641224662773 0.0357572339594277 -1.14663914385064 6.08915041092458e-18 miRanda -0.107782773565761 0.0016907871459656 NA NA NA hsa-miR-590-3p FGFR1 1.27438512890514 1.03164723481469e-23 -1.14663914385064 6.08915041092458e-18 miRanda;mirMAP -0.232790337775512 2.91176217126465e-10 NA NA NA hsa-miR-93-5p FGFR1 1.01788600209314 1.24312921618126e-21 -1.14663914385064 6.08915041092458e-18 mirMAP -0.155396198762809 0.000265353923933065 NA NA NA hsa-miR-107 FGFR2 0.678998026350662 2.56224148930538e-16 -0.775141603791194 2.04453402884716e-05 PITA;miRanda -0.355887284370132 1.72803357435902e-06 NA NA NA hsa-miR-193a-3p FGFR2 0.310617999804917 0.00477640270974957 -0.775141603791194 2.04453402884716e-05 miRanda -0.294573603452518 2.04016256999751e-07 NA NA NA hsa-miR-369-3p FGFR2 0.0880848299432269 0.48519534195924 -0.775141603791194 2.04453402884716e-05 miRNATAP -0.134882932202002 0.00703831397411665 NA NA NA hsa-miR-423-3p FGFR2 0.338351552541003 0.000947983901628776 -0.775141603791194 2.04453402884716e-05 PITA;miRNATAP -0.1727701841783 0.00483828495442655 NA NA NA hsa-miR-590-3p FGFR2 1.27438512890514 1.03164723481469e-23 -0.775141603791194 2.04453402884716e-05 miRanda -0.203907123628065 4.71460047941147e-05 NA NA NA hsa-miR-100-5p FGFR3 -1.89453309391092 5.00588682382388e-51 1.86754699235588 2.21866053477893e-14 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.235261430958759 0.000205428105475304 25344675;26604796;26018508;23778527 MicroRNA 100 regulates pancreatic cancer cells growth and sensitivity to chemotherapy through targeting FGFR3; The predicted target of miR-100 fibroblast growth factor receptor 3 FGFR3 was downregulated by siRNA to examine its effect on pancreatic cancer cells; Luciferase essay showed FGFR3 was direct target of miR-100; FGFR3 was significantly downregulated by overexpressing miR-100 in pancreatic cancer cells and knocking down FGFR3 by siRNA exerted similar effect as miR-100; Our study demonstrated that miR-100 played an important role in pancreatic cancer development possibly through targeting FGFR3;Overexpression of miR 100 inhibits cell proliferation migration and chemosensitivity in human glioblastoma through FGFR3; Expression of fibroblast growth factor receptor 3 FGFR3 the bioinformatically predicted target of miR-100 was examined by Western blot in glioblastoma; FGFR3 was directly regulated by miR-100 in glioblastoma; Ectopically overexpressing FGFR3 was able to ameliorate the anticancer effects of upregulation of miR-100 on glioblastoma growth migration and chemosensitivity; Overexpressing miR-100 had anticancer effects on glioblastoma likely through regulation of FGFR3;Overexpression of miR 100 inhibits growth of osteosarcoma through FGFR3; Here we reported significantly higher levels of fibroblast growth factor receptor 3 FGFR3 and significantly lower levels of miR-100 in the OS specimen compared to those in the paired normal bone tissues; Bioinformatics analysis and luciferase reporter assay suggest that miR-100 binds to the 3'UTR of FGFR3 mRNA to prevent its translation; Taken together our data demonstrate that miR-100 may inhibit the growth of OS through FGFR3;Hypoxia regulates FGFR3 expression via HIF 1α and miR 100 and contributes to cell survival in non muscle invasive bladder cancer; We have previously investigated the role of microRNAs in bladder cancer and have shown that FGFR3 is a target of miR-100; In this study we investigated the effects of hypoxia on miR-100 and FGFR3 expression and the link between miR-100 and FGFR3 in hypoxia; Bladder cancer cell lines were exposed to normoxic or hypoxic conditions and examined for the expression of FGFR3 by quantitative PCR qPCR and western blotting and miR-100 by qPCR; The effect of FGFR3 and miR-100 on cell viability in two-dimensional 2-D and three-dimensional 3-D was examined by transfecting siRNA or mimic-100 respectively; Increased FGFR3 was also in part dependent on miR-100 levels which decreased in hypoxia; Hypoxia in part via suppression of miR-100 induces FGFR3 expression in bladder cancer both of which have an important role in maintaining cell viability under conditions of stress ;;;poor survival pancreatic cancer;glioblastoma;sarcoma;bladder cancer hsa-miR-142-3p FGFR3 1.53951237846104 2.06769936282034e-17 1.86754699235588 2.21866053477893e-14 miRanda -0.186690154425788 4.78356753122835e-05 NA NA NA hsa-miR-19a-3p FGFR3 0.817507952171848 3.76285090469625e-08 1.86754699235588 2.21866053477893e-14 mirMAP -0.197762599825557 0.000503996109696735 NA NA NA hsa-miR-19b-3p FGFR3 0.139124391010031 0.255658553872934 1.86754699235588 2.21866053477893e-14 mirMAP -0.298900555145995 1.74346769891144e-05 NA NA NA hsa-miR-24-3p FGFR3 -0.120123317289941 0.21432376743243 1.86754699235588 2.21866053477893e-14 MirTarget;miRNATAP -0.593260915828542 1.24772160110599e-11 NA NA NA hsa-miR-337-3p FGFR3 -2.14274617598363 2.70942896809734e-43 1.86754699235588 2.21866053477893e-14 MirTarget -0.276342803887382 8.88392937600617e-08 NA NA NA hsa-miR-455-3p FGFR3 0.678392920628872 0.000327758844555877 1.86754699235588 2.21866053477893e-14 PITA -0.311970748789501 2.16597532806041e-12 NA NA NA hsa-miR-532-5p FGFR3 0.0386976222860582 0.682018574136515 1.86754699235588 2.21866053477893e-14 mirMAP -0.285596505551977 0.00164247472635195 NA NA NA hsa-miR-99a-5p FGFR3 -2.25079447371102 1.14086813185885e-54 1.86754699235588 2.21866053477893e-14 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.320837636799125 4.30235413296182e-09 23409016;24456664;23298836 Photofrin based photodynamic therapy and miR 99a transfection inhibited FGFR3 and PI3K/Akt signaling mechanisms to control growth of human glioblastoma In vitro and in vivo; Further photofrin based PDT followed by miR-99a transfection dramatically increased miR-99a expression and also increased apoptosis in glioblastoma cell cultures and drastically reduced tumor growth in athymic nude mice due to down regulation of fibroblast growth factor receptor 3 FGFR3 and PI3K/Akt signaling mechanisms leading to inhibition of cell proliferation and induction of molecular mechanisms of apoptosis;miR 99a promotes proliferation targeting FGFR3 in human epithelial ovarian cancer cells; Importantly fibroblast growth factor receptor 3 FGFR3 predicted to be one target gene of miR-99a using computational algorithms was higher in expression in EOC cells; Subsequently FGFR3 was proved to be direct target of miR-99a by dual luciferase assay; Furthermore overexpression of miR-99a dramatically suppressed expression level of FGFR3 at both mRNA and protein levels proving FGFR3 to be inversely correlated with miR-99a; Finally overexpression of miR-99a could significantly inhibit EOC cell proliferation in vitro by decreasing the expression of FGFR3 which also reduced the EOC cell growth after siRNA knockdown; Conclusively miR-99a expression was remarkably downregulated in serums tissues and cell and suppresses EOC cell proliferation by targeting FGFR3 suggesting miR-99a as a prospective prognosis marker and potential tumor suppressor for EOC therapeutics;The tumorigenic FGFR3 TACC3 gene fusion escapes miR 99a regulation in glioblastoma; The fusion caused by tandem duplication on 4p16.3 led to the loss of the 3'-UTR of FGFR3 blocking gene regulation of miR-99a and enhancing expression of the fusion gene ;worse prognosis; glioblastoma;ovarian cancer;glioblastoma hsa-miR-326 FGFR4 -1.54449030848141 1.04703417845594e-17 1.53418109992094 1.4752304353364e-09 miRanda;miRNATAP -0.170195226279497 0.000361551375916332 NA NA NA hsa-miR-491-5p FGFR4 -1.04307210952796 1.14586501470398e-15 1.53418109992094 1.4752304353364e-09 miRanda -0.298147283214882 7.57138894516634e-06 NA NA NA hsa-miR-106b-5p FLT1 1.22477789913175 2.31787213143326e-32 -0.976682151789582 4.79604479745106e-23 MirTarget;miRNATAP -0.268178565619622 3.8111441429214e-17 NA NA NA hsa-miR-107 FLT1 0.678998026350662 2.56224148930538e-16 -0.976682151789582 4.79604479745106e-23 miRanda -0.314938269682321 1.32029901450643e-14 NA NA NA hsa-miR-146b-5p FLT1 0.631041661167954 6.20366593452965e-08 -0.976682151789582 4.79604479745106e-23 miRanda -0.202091696389093 5.72871952887742e-12 NA NA NA hsa-miR-155-5p FLT1 1.09766314984151 4.55650930885814e-15 -0.976682151789582 4.79604479745106e-23 miRNAWalker2_validate;mirMAP -0.141999192123706 4.82181118961184e-09 NA NA NA hsa-miR-17-5p FLT1 0.599531757298529 1.07318660415906e-05 -0.976682151789582 4.79604479745106e-23 MirTarget;TargetScan;miRNATAP -0.16218378079303 1.23634449026838e-10 NA NA NA hsa-miR-181a-5p FLT1 0.635863607630183 6.72486918157744e-11 -0.976682151789582 4.79604479745106e-23 miRNATAP -0.219297738042611 4.37776931001255e-10 NA NA NA hsa-miR-181b-5p FLT1 1.22749257795094 1.21998198830919e-27 -0.976682151789582 4.79604479745106e-23 miRNATAP -0.222578263194722 4.64458789140547e-14 NA NA NA hsa-miR-181c-5p FLT1 0.574847839298605 4.51287502066478e-07 -0.976682151789582 4.79604479745106e-23 miRNATAP -0.239615399546846 1.17848772957282e-15 NA NA NA hsa-miR-19a-3p FLT1 0.817507952171848 3.76285090469625e-08 -0.976682151789582 4.79604479745106e-23 MirTarget -0.158919931608538 4.57034977071252e-12 NA NA NA hsa-miR-19b-3p FLT1 0.139124391010031 0.255658553872934 -0.976682151789582 4.79604479745106e-23 MirTarget -0.152764572510247 6.91840199554879e-08 NA NA NA hsa-miR-200b-3p FLT1 2.11744072703209 1.66710572104191e-43 -0.976682151789582 4.79604479745106e-23 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan -0.237387253520266 3.06104626134544e-30 21115742 Forced miR-200 expression suppressed Flt1 levels in metastasis-prone lung adenocarcinoma cells derived from K-ras/p53-mutant mice and negatively regulated the Flt1 3'-UTR in reporter assays; We conclude that miR-200 suppresses lung tumorigenesis by targeting Flt1 metastasis;tumorigenesis lung cancer hsa-miR-200c-3p FLT1 2.07038651806183 6.84613386286599e-51 -0.976682151789582 4.79604479745106e-23 miRNAWalker2_validate;miRTarBase;MirTarget -0.255668357647663 5.50361375778068e-29 NA NA NA hsa-miR-20a-5p FLT1 0.44979911120222 0.000602386964327228 -0.976682151789582 4.79604479745106e-23 MirTarget;miRNATAP -0.233663438164218 2.1323989806554e-19 NA NA NA hsa-miR-361-5p FLT1 -0.0996867547900457 0.095428250126326 -0.976682151789582 4.79604479745106e-23 miRanda -0.281387154816715 1.81346924134673e-06 NA NA NA hsa-miR-429 FLT1 2.84108892259147 3.88520896426515e-64 -0.976682151789582 4.79604479745106e-23 MirTarget;miRanda;miRNATAP -0.212494365543953 2.16632813796484e-30 NA NA NA hsa-miR-590-3p FLT1 1.27438512890514 1.03164723481469e-23 -0.976682151789582 4.79604479745106e-23 miRanda -0.150457515693858 5.7105738196085e-08 NA NA NA hsa-miR-93-5p FLT1 1.01788600209314 1.24312921618126e-21 -0.976682151789582 4.79604479745106e-23 MirTarget;miRNATAP -0.305409002203918 9.83786412000852e-23 NA NA NA hsa-miR-590-3p FLT3 1.27438512890514 1.03164723481469e-23 0.668821394707615 0.0177967298375228 PITA;miRanda;mirMAP;miRNATAP -0.209971805478275 0.00685581280914592 NA NA NA hsa-miR-491-5p FLT3LG -1.04307210952796 1.14586501470398e-15 -0.341567133322199 0.00313331665479677 miRanda -0.167637903921863 2.255502889352e-08 NA NA NA hsa-miR-331-3p FLT4 0.74441480542886 1.17071235477799e-07 -1.21716420627977 1.17202457914118e-32 miRNAWalker2_validate -0.256733379585136 1.34101220391357e-24 NA NA NA hsa-miR-429 FLT4 2.84108892259147 3.88520896426515e-64 -1.21716420627977 1.17202457914118e-32 miRanda;miRNATAP -0.220062777103595 1.23886320860694e-29 NA NA NA hsa-let-7g-5p FN1 0.0200025784917734 0.791635298815256 2.70001074183699 2.543883061007e-54 miRTarBase -0.225052184089239 0.0083113477930263 NA NA NA hsa-miR-1-3p FN1 -1.92761220689044 2.14541307073875e-29 2.70001074183699 2.543883061007e-54 miRTarBase;MirTarget -0.1494579156962 3.5893984502932e-05 NA NA NA hsa-miR-139-5p FN1 -3.25846663863766 2.62018989415003e-112 2.70001074183699 2.543883061007e-54 miRanda -0.510579303686055 6.29670431922458e-45 NA NA NA hsa-miR-193a-5p FN1 -1.1508321886378 7.11949564429669e-37 2.70001074183699 2.543883061007e-54 miRNATAP -0.201454473655291 0.00288108647203586 NA NA NA hsa-miR-217 FN1 -0.616728851115349 8.64977119332241e-05 2.70001074183699 2.543883061007e-54 MirTarget;PITA;miRanda -0.127242963083369 0.00168826238282668 NA NA NA hsa-miR-26b-5p FN1 -0.331527896896866 0.000551258576575092 2.70001074183699 2.543883061007e-54 miRNAWalker2_validate -0.298684140290862 7.00186998284315e-06 NA NA NA hsa-miR-27a-5p FN1 -1.23847974615121 1.26367431345318e-19 2.70001074183699 2.543883061007e-54 MirTarget -0.220835464335831 1.24127004151762e-06 NA NA NA hsa-miR-320a FN1 -0.287921276129346 0.00141693637596294 2.70001074183699 2.543883061007e-54 miRanda -0.339258614542988 1.62507196664897e-06 NA NA NA hsa-miR-511-5p FN1 -2.27545475897362 3.84085514050558e-36 2.70001074183699 2.543883061007e-54 MirTarget -0.10476429635028 0.00200343663280643 NA NA NA hsa-miR-103a-3p FOXO3 0.610673390034345 1.63113920514646e-12 -0.569903527602583 2.86129588559767e-12 mirMAP -0.257373634353798 8.37424745833717e-16 NA NA NA hsa-miR-107 FOXO3 0.678998026350662 2.56224148930538e-16 -0.569903527602583 2.86129588559767e-12 miRanda;mirMAP -0.241737835867968 4.11362198115301e-13 NA NA NA hsa-miR-221-3p FOXO3 -0.317970198201501 0.00910855267413448 -0.569903527602583 2.86129588559767e-12 miRNAWalker2_validate;miRTarBase -0.102971824212777 8.79537054419204e-06 NA NA NA hsa-miR-27a-3p FOXO3 0.388496361379095 1.42373532086331e-05 -0.569903527602583 2.86129588559767e-12 miRNATAP -0.12585799199065 6.82808873003821e-05 23621269 FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes glioblastoma hsa-miR-29b-3p FOXO3 0.438847962603729 6.46722433236121e-05 -0.569903527602583 2.86129588559767e-12 miRNATAP -0.117477827392279 4.69947625496637e-06 NA NA NA hsa-miR-29c-3p FOXO3 -0.0652732378039769 0.563774115756922 -0.569903527602583 2.86129588559767e-12 miRNATAP -0.126340887444201 4.3495643844148e-07 NA NA NA hsa-miR-340-5p FOXO3 1.0857159627596 4.94471062546756e-24 -0.569903527602583 2.86129588559767e-12 mirMAP -0.115779071352587 6.22915321942398e-06 NA NA NA hsa-miR-361-3p FOXO3 -0.0370446538644886 0.624902392829872 -0.569903527602583 2.86129588559767e-12 miRNATAP -0.210072128381716 2.12730489785468e-08 NA NA NA hsa-miR-142-3p GHR 1.53951237846104 2.06769936282034e-17 -3.36237154973502 5.01584120795748e-76 MirTarget;PITA;miRanda;miRNATAP -0.258278368150427 1.97065873638164e-12 NA NA NA hsa-miR-15a-5p GHR 0.812445324157528 4.50263417099822e-20 -3.36237154973502 5.01584120795748e-76 MirTarget;miRNATAP -0.605293472852955 7.30489418651164e-16 NA NA NA hsa-miR-15b-5p GHR 0.788659356634696 1.13678211092534e-11 -3.36237154973502 5.01584120795748e-76 MirTarget;miRNATAP -0.400222488614987 4.86555636936937e-12 NA NA NA hsa-miR-16-2-3p GHR 0.286355254959005 0.0256946046884474 -3.36237154973502 5.01584120795748e-76 mirMAP -0.302593175048704 1.65900338359926e-08 NA NA NA hsa-miR-16-5p GHR 0.46835991343556 1.91125356547776e-06 -3.36237154973502 5.01584120795748e-76 MirTarget;miRNATAP -0.35025844837926 4.66351956117755e-07 NA NA NA hsa-miR-29a-5p GHR 0.334183773952055 0.00445137265870197 -3.36237154973502 5.01584120795748e-76 mirMAP;miRNATAP -0.336628492524444 7.8070583731355e-09 NA NA NA hsa-miR-424-5p GHR 0.420789584199235 0.00480101660479489 -3.36237154973502 5.01584120795748e-76 MirTarget;miRNATAP -0.129234384065226 0.00501663875372727 NA NA NA hsa-miR-429 GHR 2.84108892259147 3.88520896426515e-64 -3.36237154973502 5.01584120795748e-76 miRanda;miRNATAP -0.565426163436487 1.88289719909835e-57 NA NA NA hsa-miR-455-5p GHR 0.284641224662773 0.0357572339594277 -3.36237154973502 5.01584120795748e-76 miRanda -0.358213904373597 9.36049755011874e-13 NA NA NA hsa-miR-590-3p GHR 1.27438512890514 1.03164723481469e-23 -3.36237154973502 5.01584120795748e-76 mirMAP -0.559291311133228 1.52163936994309e-25 NA NA NA hsa-miR-7-1-3p GHR 0.686579567507582 1.20731554448806e-07 -3.36237154973502 5.01584120795748e-76 MirTarget;mirMAP -0.252886866186807 1.44233263663563e-06 NA NA NA hsa-miR-23a-3p GNB2 -0.217176729033818 0.00962615056202409 0.88241249965083 1.56989231821362e-33 miRNAWalker2_validate -0.223826338992611 4.29157165949292e-13 NA NA NA hsa-miR-30b-3p GNB3 -0.536462824497982 2.41814659272548e-05 -0.218781005289026 0.156021511354067 MirTarget -0.10995518264951 0.00843692403427671 NA NA NA hsa-miR-326 GNB3 -1.54449030848141 1.04703417845594e-17 -0.218781005289026 0.156021511354067 miRanda -0.159948449709777 2.11022567862306e-08 NA NA NA hsa-miR-330-5p GNB3 0.626063225953928 3.42215028606779e-09 -0.218781005289026 0.156021511354067 miRanda -0.169128654107362 0.000640956496089397 NA NA NA hsa-miR-491-5p GNB3 -1.04307210952796 1.14586501470398e-15 -0.218781005289026 0.156021511354067 miRanda -0.157999452002575 8.19626078438683e-05 NA NA NA hsa-miR-29c-3p GNB4 -0.0652732378039769 0.563774115756922 -0.0279351136019024 0.79353569056173 miRNAWalker2_validate;MirTarget -0.301018105639441 1.91560837681814e-21 NA NA NA hsa-miR-361-5p GNB4 -0.0996867547900457 0.095428250126326 -0.0279351136019024 0.79353569056173 miRanda;miRNATAP -0.177421789581256 0.00432005891214685 NA NA NA hsa-miR-128-3p GNB5 0.90296715955238 9.925204823863e-23 -0.407104708333545 6.5228202573912e-07 miRNAWalker2_validate -0.114359353671188 0.000132372943896389 NA NA NA hsa-miR-130b-3p GNB5 1.38122163823846 1.00932179737836e-26 -0.407104708333545 6.5228202573912e-07 miRNAWalker2_validate -0.138451761651205 5.12903842421723e-11 NA NA NA hsa-miR-17-5p GNB5 0.599531757298529 1.07318660415906e-05 -0.407104708333545 6.5228202573912e-07 TargetScan -0.174552122552597 6.18835184294834e-18 NA NA NA hsa-miR-425-5p GNB5 0.719898949241718 6.01079610190374e-08 -0.407104708333545 6.5228202573912e-07 miRNAWalker2_validate -0.128350915841136 8.24892867293889e-10 NA NA NA hsa-miR-590-3p GNB5 1.27438512890514 1.03164723481469e-23 -0.407104708333545 6.5228202573912e-07 miRanda -0.130708855700002 5.67204097508921e-09 NA NA NA hsa-miR-320b GNG11 0.412787504747938 0.00113457276903985 -2.08770328232076 1.27194301059522e-79 miRanda -0.183001196145562 2.81304752037874e-08 NA NA NA hsa-miR-330-5p GNG11 0.626063225953928 3.42215028606779e-09 -2.08770328232076 1.27194301059522e-79 miRanda -0.363851891974706 2.48648847018044e-21 NA NA NA hsa-miR-107 GNG12 0.678998026350662 2.56224148930538e-16 -1.00662202078145 1.13075382846906e-21 miRanda -0.551701699322264 2.91176766840327e-39 NA NA NA hsa-miR-30d-3p GNG12 0.264544633563642 0.00755023189129747 -1.00662202078145 1.13075382846906e-21 mirMAP -0.208468484284917 2.20758275785736e-08 NA NA NA hsa-miR-374a-5p GNG12 -0.239709888246804 0.00309819667153627 -1.00662202078145 1.13075382846906e-21 mirMAP -0.224009250033915 9.67825178709072e-07 NA NA NA hsa-miR-374b-5p GNG12 -0.466781429946153 1.17238311619116e-08 -1.00662202078145 1.13075382846906e-21 mirMAP -0.149043145224379 0.000959250373367468 NA NA NA hsa-miR-423-5p GNG12 -0.0644843381453004 0.462275547464843 -1.00662202078145 1.13075382846906e-21 miRNAWalker2_validate -0.232418945817642 3.66766754252117e-08 NA NA NA hsa-miR-429 GNG12 2.84108892259147 3.88520896426515e-64 -1.00662202078145 1.13075382846906e-21 miRanda -0.170551562257512 1.90032220822832e-17 NA NA NA hsa-miR-455-5p GNG12 0.284641224662773 0.0357572339594277 -1.00662202078145 1.13075382846906e-21 miRanda -0.136447911297092 5.25157686814491e-07 NA NA NA hsa-miR-508-3p GNG12 0.260698649973977 0.189926238144551 -1.00662202078145 1.13075382846906e-21 mirMAP -0.121287386585328 6.28721215718235e-11 NA NA NA hsa-miR-542-3p GNG12 0.568822103820889 8.5699075257651e-07 -1.00662202078145 1.13075382846906e-21 miRanda -0.112752886973826 0.000401656809905115 NA NA NA hsa-miR-590-3p GNG12 1.27438512890514 1.03164723481469e-23 -1.00662202078145 1.13075382846906e-21 miRanda;mirMAP -0.230577467147612 2.85613249871964e-15 NA NA NA hsa-miR-590-5p GNG12 0.746515607868274 8.51300479640125e-09 -1.00662202078145 1.13075382846906e-21 PITA -0.177407665286786 2.67698443222439e-10 NA NA NA hsa-miR-96-5p GNG12 3.24596558166606 1.92296159041707e-98 -1.00662202078145 1.13075382846906e-21 TargetScan -0.177675699622518 2.20766570176543e-17 NA NA NA hsa-miR-511-5p GNG13 -2.27545475897362 3.84085514050558e-36 3.60156617640628 6.90742745616058e-33 MirTarget -0.512118109882972 5.35501018269789e-20 NA NA NA hsa-miR-181a-5p GNG2 0.635863607630183 6.72486918157744e-11 -1.9182405505051 4.75840171347434e-62 miRNATAP -0.158622872266113 0.000268518996801588 NA NA NA hsa-miR-181b-5p GNG2 1.22749257795094 1.21998198830919e-27 -1.9182405505051 4.75840171347434e-62 miRNATAP -0.242792923107725 2.59948071095444e-11 NA NA NA hsa-miR-181c-5p GNG2 0.574847839298605 4.51287502066478e-07 -1.9182405505051 4.75840171347434e-62 miRNATAP -0.238454563522782 1.20468334925699e-10 NA NA NA hsa-miR-21-5p GNG2 2.32007501846389 3.08394781855554e-137 -1.9182405505051 4.75840171347434e-62 miRNATAP -0.40885794761468 1.88540041022037e-27 NA NA NA hsa-miR-29b-3p GNG2 0.438847962603729 6.46722433236121e-05 -1.9182405505051 4.75840171347434e-62 miRNATAP -0.177191825291211 4.85752847508701e-06 NA NA NA hsa-miR-30d-3p GNG2 0.264544633563642 0.00755023189129747 -1.9182405505051 4.75840171347434e-62 mirMAP -0.314140730350831 2.09757667757834e-13 NA NA NA hsa-miR-324-5p GNG2 0.211552647440779 0.139408130311654 -1.9182405505051 4.75840171347434e-62 miRanda -0.111829031139688 0.000180436111790897 NA NA NA hsa-miR-330-5p GNG2 0.626063225953928 3.42215028606779e-09 -1.9182405505051 4.75840171347434e-62 miRanda -0.128481680756102 0.00136130772027182 NA NA NA hsa-miR-429 GNG2 2.84108892259147 3.88520896426515e-64 -1.9182405505051 4.75840171347434e-62 miRanda;miRNATAP -0.366896431796571 2.59270002688012e-63 NA NA NA hsa-miR-590-3p GNG2 1.27438512890514 1.03164723481469e-23 -1.9182405505051 4.75840171347434e-62 PITA;miRanda;mirMAP;miRNATAP -0.176213741742696 2.38118796696407e-07 NA NA NA hsa-miR-590-5p GNG2 0.746515607868274 8.51300479640125e-09 -1.9182405505051 4.75840171347434e-62 miRanda;miRNATAP -0.162596484872571 6.15767106446387e-07 NA NA NA hsa-miR-218-5p GNG3 -1.02621788318234 2.9221925445771e-23 1.11699723966564 1.27168600858717e-10 MirTarget -0.29417055790856 2.01234117411904e-07 NA NA NA hsa-let-7a-5p GNG5 -0.210286642041636 0.0072546234707717 0.720669896372741 1.33713375996743e-23 MirTarget;TargetScan;miRNATAP -0.263609332795226 1.89983891846224e-16 NA NA NA hsa-let-7b-5p GNG5 -0.54215483448257 7.21347617597858e-08 0.720669896372741 1.33713375996743e-23 MirTarget;miRNATAP -0.215365938729338 1.98107309244769e-18 NA NA NA hsa-let-7c-5p GNG5 -1.9415540533505 9.55250745007793e-48 0.720669896372741 1.33713375996743e-23 MirTarget -0.130308599771332 1.4272369414858e-13 NA NA NA hsa-miR-100-5p GNG5 -1.89453309391092 5.00588682382388e-51 0.720669896372741 1.33713375996743e-23 miRNAWalker2_validate -0.170666649924024 2.31239110416299e-20 NA NA NA hsa-miR-199a-5p GNG5 -0.314703593105081 0.00909304983051124 0.720669896372741 1.33713375996743e-23 MirTarget;miRanda;miRNATAP -0.103725476670797 7.1766087314945e-07 NA NA NA hsa-miR-141-3p GNG7 2.99777540204168 1.26370498493024e-78 -1.27893544175867 1.22567787829192e-21 MirTarget;TargetScan;miRNATAP -0.160816727393679 1.22991601143051e-09 NA NA NA hsa-miR-182-5p GNG7 2.36408084103818 2.60011457600281e-72 -1.27893544175867 1.22567787829192e-21 mirMAP -0.192436587268947 3.0582156776103e-09 NA NA NA hsa-miR-185-3p GNG7 0.86591374114446 7.8790769678542e-10 -1.27893544175867 1.22567787829192e-21 mirMAP -0.277859437819126 4.04643911165602e-17 NA NA NA hsa-miR-18a-5p GNG7 0.969066793715868 1.69069796425892e-08 -1.27893544175867 1.22567787829192e-21 mirMAP -0.102334215711126 0.000163584996294418 NA NA NA hsa-miR-193b-5p GNG7 -0.481487407783336 0.000206895781789619 -1.27893544175867 1.22567787829192e-21 mirMAP -0.208151006145478 7.31519163743523e-09 NA NA NA hsa-miR-1976 GNG7 0.289190368864725 0.00167092758216584 -1.27893544175867 1.22567787829192e-21 mirMAP -0.300152613953244 3.81622005339607e-09 NA NA NA hsa-miR-22-5p GNG7 0.238328801322329 0.0377496760295305 -1.27893544175867 1.22567787829192e-21 mirMAP -0.260659828457892 1.55588475652111e-10 NA NA NA hsa-miR-23a-3p GNG7 -0.217176729033818 0.00962615056202409 -1.27893544175867 1.22567787829192e-21 mirMAP -0.241969817588217 1.71784058793468e-05 NA NA NA hsa-miR-29b-3p GNG7 0.438847962603729 6.46722433236121e-05 -1.27893544175867 1.22567787829192e-21 mirMAP -0.166028116001799 0.000102220857349818 NA NA NA hsa-miR-3127-5p GNG7 0.903405128081314 1.1325923598273e-12 -1.27893544175867 1.22567787829192e-21 mirMAP -0.264037103319252 2.7885903673376e-13 NA NA NA hsa-miR-330-5p GNG7 0.626063225953928 3.42215028606779e-09 -1.27893544175867 1.22567787829192e-21 mirMAP -0.360143214353564 1.04384209007179e-16 NA NA NA hsa-miR-532-3p GNG7 -0.395473145042459 0.00686714288452761 -1.27893544175867 1.22567787829192e-21 mirMAP -0.134877459760651 2.59162844952196e-05 NA NA NA hsa-miR-590-3p GNG7 1.27438512890514 1.03164723481469e-23 -1.27893544175867 1.22567787829192e-21 PITA;miRanda;mirMAP -0.234865237619927 3.35435901958928e-10 NA NA NA hsa-miR-671-5p GNG7 1.55248047544015 7.7377784125886e-33 -1.27893544175867 1.22567787829192e-21 mirMAP -0.243406330452064 3.86780682385241e-12 NA NA NA hsa-miR-766-3p GNG7 0.496790679032227 8.22686414022553e-05 -1.27893544175867 1.22567787829192e-21 MirTarget -0.263281448404197 8.01439191181685e-13 NA NA NA hsa-miR-96-5p GNG7 3.24596558166606 1.92296159041707e-98 -1.27893544175867 1.22567787829192e-21 mirMAP -0.189536520051665 1.57219350207875e-12 NA NA NA hsa-miR-100-5p GRB2 -1.89453309391092 5.00588682382388e-51 0.639828372471576 5.6864573011903e-23 miRNAWalker2_validate -0.156661486141178 3.5519143452408e-21 NA NA NA hsa-miR-378a-3p GRB2 -1.79882978380301 1.05005373951733e-38 0.639828372471576 5.6864573011903e-23 miRNAWalker2_validate;MirTarget;miRNATAP -0.128042134644829 1.17944311772052e-16 NA NA NA hsa-miR-378c GRB2 -1.79697571999492 1.07363220292403e-41 0.639828372471576 5.6864573011903e-23 MirTarget;miRNATAP -0.126006904107165 4.99041375742704e-15 NA NA NA hsa-miR-101-3p GSK3B -0.126035286271986 0.145997832808138 0.16507499183074 0.0104993269942212 miRNATAP -0.1804025732635 1.07790099640944e-12 NA NA NA hsa-miR-181a-5p GSK3B 0.635863607630183 6.72486918157744e-11 0.16507499183074 0.0104993269942212 mirMAP -0.116539047078398 1.93667948800215e-07 NA NA NA hsa-miR-26a-5p GSK3B -0.481180084102235 1.82368246174758e-10 0.16507499183074 0.0104993269942212 miRNAWalker2_validate;miRNATAP -0.275908606747704 3.58443650423207e-22 NA NA NA hsa-miR-29a-3p GSK3B -0.699539666833076 1.13935003139957e-13 0.16507499183074 0.0104993269942212 miRNATAP -0.20395154397803 2.73691130456682e-19 NA NA NA hsa-miR-29c-3p GSK3B -0.0652732378039769 0.563774115756922 0.16507499183074 0.0104993269942212 miRNATAP -0.148921210430456 1.24088422020447e-14 NA NA NA hsa-miR-320a GSK3B -0.287921276129346 0.00141693637596294 0.16507499183074 0.0104993269942212 miRanda;mirMAP -0.129942826481556 1.0429094971177e-07 NA NA NA hsa-miR-340-5p GSK3B 1.0857159627596 4.94471062546756e-24 0.16507499183074 0.0104993269942212 mirMAP -0.119708017508741 1.77864671732598e-09 NA NA NA hsa-miR-374a-5p GSK3B -0.239709888246804 0.00309819667153627 0.16507499183074 0.0104993269942212 mirMAP -0.161835653986741 2.7137832607523e-09 NA NA NA hsa-miR-542-3p GSK3B 0.568822103820889 8.5699075257651e-07 0.16507499183074 0.0104993269942212 miRanda -0.110477181605807 5.02640445089489e-09 NA NA NA hsa-let-7a-3p HGF 0.466622567891891 3.70886257943079e-07 -1.50227719275535 2.60659514703213e-20 mirMAP -0.37044217111857 1.87158404007688e-09 NA NA NA hsa-miR-141-3p HGF 2.99777540204168 1.26370498493024e-78 -1.50227719275535 2.60659514703213e-20 MirTarget;TargetScan -0.460530345225581 6.82815824837891e-52 NA NA NA hsa-miR-181a-5p HGF 0.635863607630183 6.72486918157744e-11 -1.50227719275535 2.60659514703213e-20 mirMAP -0.229180145731049 7.99295141524896e-05 NA NA NA hsa-miR-181b-5p HGF 1.22749257795094 1.21998198830919e-27 -1.50227719275535 2.60659514703213e-20 mirMAP -0.309758876766791 1.99330459381299e-10 NA NA NA hsa-miR-181c-5p HGF 0.574847839298605 4.51287502066478e-07 -1.50227719275535 2.60659514703213e-20 mirMAP -0.325896473603488 4.31927246381495e-11 NA NA NA hsa-miR-200a-3p HGF 2.55218480823434 2.96135446816238e-51 -1.50227719275535 2.60659514703213e-20 MirTarget -0.395550183948552 6.66851532585504e-39 NA NA NA hsa-miR-203a-3p HGF 1.67084394806148 6.06522273352132e-14 -1.50227719275535 2.60659514703213e-20 MirTarget -0.135558542998366 6.49068618028159e-08 NA NA NA hsa-miR-29a-5p HGF 0.334183773952055 0.00445137265870197 -1.50227719275535 2.60659514703213e-20 mirMAP -0.20905674153194 1.72665124213689e-05 NA NA NA hsa-miR-30b-5p HGF -0.46540603860146 2.4709940429591e-05 -1.50227719275535 2.60659514703213e-20 mirMAP -0.165128975760674 0.00139400110552165 NA NA NA hsa-miR-30c-5p HGF -0.393222674580066 8.06961465717306e-05 -1.50227719275535 2.60659514703213e-20 mirMAP -0.148910356466254 0.00939828977159023 NA NA NA hsa-miR-30d-5p HGF -0.247082219011045 0.00406717930063148 -1.50227719275535 2.60659514703213e-20 mirMAP -0.234640404839747 0.000434087952565876 NA NA NA hsa-miR-30e-5p HGF 0.293491764699475 0.000372291967271834 -1.50227719275535 2.60659514703213e-20 mirMAP -0.36586551099525 1.24219852188022e-07 NA NA NA hsa-miR-338-3p HGF 0.0799903526884966 0.575137668326679 -1.50227719275535 2.60659514703213e-20 miRanda -0.165633335494788 3.4386025988981e-05 NA NA NA hsa-miR-429 HGF 2.84108892259147 3.88520896426515e-64 -1.50227719275535 2.60659514703213e-20 miRanda -0.390119016718709 2.48108439155069e-38 NA NA NA hsa-miR-590-3p HGF 1.27438512890514 1.03164723481469e-23 -1.50227719275535 2.60659514703213e-20 miRanda;mirMAP -0.405090232781221 1.5991156344667e-19 NA NA NA hsa-miR-590-5p HGF 0.746515607868274 8.51300479640125e-09 -1.50227719275535 2.60659514703213e-20 miRanda -0.432886188104826 2.68689870271352e-24 NA NA NA hsa-miR-7-1-3p HGF 0.686579567507582 1.20731554448806e-07 -1.50227719275535 2.60659514703213e-20 mirMAP -0.201885417951517 3.66160567897647e-06 NA NA NA hsa-let-7a-5p HRAS -0.210286642041636 0.0072546234707717 0.871315771945202 9.54693368299171e-15 miRNAWalker2_validate;miRTarBase -0.156144832596741 0.00194814074250424 20607356;23134218;18344688;20033209;19323605 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells;MicroRNA let 7a inhibits the proliferation and invasion of nonsmall cell lung cancer cell line 95D by regulating K Ras and HMGA2 gene expression; K-RAS and HMGA2 mRNA levels were significantly higher in the let-7a overexpressed group than those in the let-7a inhibited group p < 0.05; However the protein levels of K-RAS and HMGA2 were significantly lower in the let-7a overexpressed group than those in the let-7a inhibited group p < 0.05; We suppose that let-7a inhibits the proliferation and invasion of the cell line 95D by regulating the translation of K-RAS and HMGA2 mRNA not the transcription of the mRNA itself;Using an established orthotopic mouse lung cancer model we show that intranasal let-7 administration reduces tumor formation in vivo in the lungs of animals expressing a G12D activating mutation for the K-ras oncogene;k-Ras and c-Myc two key oncogenes in lung cancer have been found to be targeted by let-7 in vitro; The aim of the present study is to determine the effect of let-7a a member of let-7 family on the growth of lung cancer in vivo and to investigate whether let-7-induced suppression of k-Ras and c-Myc is involved in lung cancer; Overexpression of let-7a can inhibit the growth of lung cancer transplanted subcutaneously in nude mice by suppression of k-Ras and c-Myc;Because let-7 microRNA targets the K-ras oncogene we aimed to characterize let-7 expression and function in PDAC in vitro and in vivo; Restoring let-7 levels in cancer-derived cell lines strongly inhibits cell proliferation K-ras expression and mitogen-activated protein kinase activation but fails to impede tumor growth progression after intratumoral gene transfer or after implantation of Capan-1 cells stably overexpressing let-7 microRNA ;;;;progression glioblastoma;lung squamous cell cancer;lung cancer;lung cancer;pancreatic cancer hsa-let-7b-5p HRAS -0.54215483448257 7.21347617597858e-08 0.871315771945202 9.54693368299171e-15 miRTarBase -0.245640732103343 1.64072414700874e-10 20607356;20881268;18344688;20033209;19323605 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells;On the basis of these data we suggest that the downregulation of let-7b and let-7e targeting K-ras and the upregulation of miR-17* a CRC marker could be considered as candidate molecular markers of cetuximab resistance;Using an established orthotopic mouse lung cancer model we show that intranasal let-7 administration reduces tumor formation in vivo in the lungs of animals expressing a G12D activating mutation for the K-ras oncogene;k-Ras and c-Myc two key oncogenes in lung cancer have been found to be targeted by let-7 in vitro; The aim of the present study is to determine the effect of let-7a a member of let-7 family on the growth of lung cancer in vivo and to investigate whether let-7-induced suppression of k-Ras and c-Myc is involved in lung cancer;Because let-7 microRNA targets the K-ras oncogene we aimed to characterize let-7 expression and function in PDAC in vitro and in vivo; Restoring let-7 levels in cancer-derived cell lines strongly inhibits cell proliferation K-ras expression and mitogen-activated protein kinase activation but fails to impede tumor growth progression after intratumoral gene transfer or after implantation of Capan-1 cells stably overexpressing let-7 microRNA ;drug resistance;;;progression glioblastoma;colorectal cancer;lung cancer;lung cancer;pancreatic cancer hsa-miR-143-3p HRAS -1.23248181631499 4.74160976678504e-28 0.871315771945202 9.54693368299171e-15 miRNAWalker2_validate;miRTarBase -0.13171025180707 9.59955008801748e-05 21276449 The Evi1 microRNA 143 K Ras axis in colon cancer colon cancer hsa-miR-766-3p HRAS 0.496790679032227 8.22686414022553e-05 0.871315771945202 9.54693368299171e-15 MirTarget -0.107283016649624 0.000532542374235056 NA NA NA hsa-miR-361-5p HSP90AA1 -0.0996867547900457 0.095428250126326 0.292350862138424 0.000313424861015945 MirTarget;PITA;miRanda -0.152038821849007 0.00132870230934462 NA NA NA hsa-miR-411-5p HSP90AA1 -0.975911498088906 3.12547465315205e-11 0.292350862138424 0.000313424861015945 MirTarget -0.113432613316559 1.60589036274037e-09 NA NA NA hsa-miR-139-5p IBSP -3.25846663863766 2.62018989415003e-112 5.71906594628957 7.23332970679324e-78 miRanda -1.07512158184459 1.20501314576614e-62 NA NA NA hsa-miR-199b-5p IBSP -1.37022396448377 3.25758057539223e-26 5.71906594628957 7.23332970679324e-78 miRanda -0.616681302292973 6.48501758674696e-13 NA NA NA hsa-miR-26b-5p IBSP -0.331527896896866 0.000551258576575092 5.71906594628957 7.23332970679324e-78 miRNAWalker2_validate -0.325286472919456 0.0071964232969712 NA NA NA hsa-miR-17-3p IFNAR1 0.721439431208839 7.95900548819742e-10 -0.376764183648069 3.13884253862067e-14 mirMAP -0.141624091960054 1.59714503842496e-23 NA NA NA hsa-miR-361-5p IFNAR1 -0.0996867547900457 0.095428250126326 -0.376764183648069 3.13884253862067e-14 miRanda -0.186548906123732 1.28504149793092e-10 NA NA NA hsa-miR-3913-5p IFNAR1 0.71890413546147 2.61153227160465e-13 -0.376764183648069 3.13884253862067e-14 mirMAP -0.143784211345676 3.40037571160059e-17 NA NA NA hsa-miR-342-3p IFNAR2 1.21944481975657 6.81914373001202e-19 -0.402774748171707 2.23085738240317e-07 miRanda -0.128014720473531 1.25634020519851e-11 NA NA NA hsa-let-7a-3p IGF1 0.466622567891891 3.70886257943079e-07 -2.74966407318656 1.16668946763881e-41 mirMAP -0.598494517106358 2.99480834420479e-14 NA NA NA hsa-let-7f-1-3p IGF1 0.37532726250321 0.00156021688753682 -2.74966407318656 1.16668946763881e-41 mirMAP -0.284984074850365 5.12019686555637e-06 NA NA NA hsa-miR-130b-3p IGF1 1.38122163823846 1.00932179737836e-26 -2.74966407318656 1.16668946763881e-41 MirTarget -0.632738321082739 2.08097755219068e-32 NA NA NA hsa-miR-142-3p IGF1 1.53951237846104 2.06769936282034e-17 -2.74966407318656 1.16668946763881e-41 miRanda -0.123324920262687 0.00187203848886841 NA NA NA hsa-miR-148a-5p IGF1 0.572692271754695 2.14306251522796e-06 -2.74966407318656 1.16668946763881e-41 mirMAP -0.165502731566367 0.00629257314556201 NA NA NA hsa-miR-155-5p IGF1 1.09766314984151 4.55650930885814e-15 -2.74966407318656 1.16668946763881e-41 mirMAP -0.165951614876576 0.00129161967089585 NA NA NA hsa-miR-15b-3p IGF1 1.12898579025388 1.18050912917317e-14 -2.74966407318656 1.16668946763881e-41 mirMAP -0.538169881674298 1.72833038996145e-29 NA NA NA hsa-miR-16-1-3p IGF1 1.30142784811196 2.53343713753744e-29 -2.74966407318656 1.16668946763881e-41 mirMAP -0.666577091007243 4.14343498994569e-26 NA NA NA hsa-miR-181a-5p IGF1 0.635863607630183 6.72486918157744e-11 -2.74966407318656 1.16668946763881e-41 mirMAP -0.494938478163062 2.34360673909487e-11 NA NA NA hsa-miR-181b-5p IGF1 1.22749257795094 1.21998198830919e-27 -2.74966407318656 1.16668946763881e-41 mirMAP -0.585684202347318 2.25280244287885e-21 NA NA NA hsa-miR-181c-5p IGF1 0.574847839298605 4.51287502066478e-07 -2.74966407318656 1.16668946763881e-41 mirMAP -0.395598534798654 5.11211832683976e-10 NA NA NA hsa-miR-186-5p IGF1 -0.426987301460247 1.04129380549315e-05 -2.74966407318656 1.16668946763881e-41 mirMAP -0.219700941598902 0.00375910687826014 NA NA NA hsa-miR-19a-3p IGF1 0.817507952171848 3.76285090469625e-08 -2.74966407318656 1.16668946763881e-41 MirTarget -0.513925086378424 2.94832706936413e-27 NA NA NA hsa-miR-19b-1-5p IGF1 0.647704962003062 3.07687667945597e-07 -2.74966407318656 1.16668946763881e-41 mirMAP -0.553744322104364 7.97205759882393e-23 NA NA NA hsa-miR-19b-3p IGF1 0.139124391010031 0.255658553872934 -2.74966407318656 1.16668946763881e-41 MirTarget -0.446691776662058 5.00619914718767e-14 NA NA NA hsa-miR-20a-3p IGF1 -0.218902047543766 0.179868363625547 -2.74966407318656 1.16668946763881e-41 mirMAP -0.256736318876148 1.0275917705269e-08 NA NA NA hsa-miR-27a-3p IGF1 0.388496361379095 1.42373532086331e-05 -2.74966407318656 1.16668946763881e-41 miRNAWalker2_validate;miRTarBase -0.544265988607736 2.14223289540701e-11 NA NA NA hsa-miR-29b-3p IGF1 0.438847962603729 6.46722433236121e-05 -2.74966407318656 1.16668946763881e-41 MirTarget -0.414656321477745 3.77745108303762e-10 25592039 Luciferase reporter assays were conducted to determine the association between miR-29b and the insulin-like growth factor 1 IGF1 3' untranslated region 3'UTR; IGF1 an activator of PI3K/Akt signaling was confirmed as a novel target of miR-29b colorectal cancer hsa-miR-301a-3p IGF1 1.32170168375632 2.12280368957514e-15 -2.74966407318656 1.16668946763881e-41 MirTarget -0.553869611428521 1.38070780054886e-40 NA NA NA hsa-miR-320b IGF1 0.412787504747938 0.00113457276903985 -2.74966407318656 1.16668946763881e-41 miRNATAP -0.353937956338846 8.47295336022305e-10 NA NA NA hsa-miR-362-5p IGF1 -0.248676340901797 0.0726089851456282 -2.74966407318656 1.16668946763881e-41 mirMAP -0.161368577599581 0.00235431302635106 NA NA NA hsa-miR-3913-5p IGF1 0.71890413546147 2.61153227160465e-13 -2.74966407318656 1.16668946763881e-41 mirMAP -0.344116396893892 3.11162487969542e-06 NA NA NA hsa-miR-454-3p IGF1 1.28177672191095 6.84024979484587e-21 -2.74966407318656 1.16668946763881e-41 MirTarget -0.592555267211157 2.36575948576207e-31 NA NA NA hsa-miR-576-5p IGF1 0.0649387707567879 0.588791966096287 -2.74966407318656 1.16668946763881e-41 PITA;mirMAP;miRNATAP -0.338610461178183 2.7172717713968e-08 NA NA NA hsa-miR-590-3p IGF1 1.27438512890514 1.03164723481469e-23 -2.74966407318656 1.16668946763881e-41 MirTarget;miRanda;mirMAP;miRNATAP -0.58985675364928 6.40681378251621e-25 NA NA NA hsa-miR-629-5p IGF1 0.42411327890895 2.46262300605706e-05 -2.74966407318656 1.16668946763881e-41 mirMAP -0.48961361687082 1.21612287765371e-11 NA NA NA hsa-let-7c-5p IGF1R -1.9415540533505 9.55250745007793e-48 0.118209603036572 0.482168850037006 MirTarget -0.15218155714742 0.000174427683240424 NA NA NA hsa-let-7d-5p IGF1R 0.410958462607207 6.07808182756787e-06 0.118209603036572 0.482168850037006 miRNAWalker2_validate;MirTarget;miRNATAP -0.250052725996837 7.84216944881654e-05 25287248 Focusing on the let-7d-5p/3p pair the respectively targeted IGF1R and KRAS were shown to be in a reverse relationship with expression of the respective miRNA which was confirmed in transient transfection assays using let-7d mimic or inhibitor; Targeting of KRAS by let-7d was previous reported; targeting of IGF1R by let-7d-5p was confirmed in luciferase assays in this study colon cancer hsa-let-7i-5p IGF1R -0.333419326258177 3.56960810786801e-08 0.118209603036572 0.482168850037006 MirTarget;miRNATAP -0.668659308812613 1.79577155658529e-12 NA NA NA hsa-miR-140-3p IGF1R -1.25844395244141 5.29222651039892e-33 0.118209603036572 0.482168850037006 mirMAP -0.257373015650615 9.61550249349614e-07 24039995 Integrated analysis identified IGF1R as a direct and functional target of miR-140; Knockdown of IGF1R inhibited cell proliferation and invasion resembling that of miR-140 overexpression while overexpression of IGF1R attenuated the function of miR-140 in NSCLC cells; Together our results highlight the significance of miR-140 and IGF1R in the development and progression of NSCLC progression lung squamous cell cancer hsa-miR-140-5p IGF1R -0.755971041589807 9.68060922934542e-14 0.118209603036572 0.482168850037006 PITA;miRNATAP -0.148653480588664 0.0080741246036404 24039995 Integrated analysis identified IGF1R as a direct and functional target of miR-140; Knockdown of IGF1R inhibited cell proliferation and invasion resembling that of miR-140 overexpression while overexpression of IGF1R attenuated the function of miR-140 in NSCLC cells; Together our results highlight the significance of miR-140 and IGF1R in the development and progression of NSCLC progression lung squamous cell cancer hsa-miR-146b-5p IGF1R 0.631041661167954 6.20366593452965e-08 0.118209603036572 0.482168850037006 miRanda -0.476741215566141 2.27302563692138e-23 NA NA NA hsa-miR-148a-5p IGF1R 0.572692271754695 2.14306251522796e-06 0.118209603036572 0.482168850037006 mirMAP -0.391801977453846 4.46869543973991e-17 NA NA NA hsa-miR-150-5p IGF1R -0.832049526091211 3.45299201139224e-05 0.118209603036572 0.482168850037006 mirMAP -0.142283823812173 5.38370164694848e-07 NA NA NA hsa-miR-155-5p IGF1R 1.09766314984151 4.55650930885814e-15 0.118209603036572 0.482168850037006 mirMAP -0.389821123327602 4.78445816137562e-23 NA NA NA hsa-miR-15a-5p IGF1R 0.812445324157528 4.50263417099822e-20 0.118209603036572 0.482168850037006 miRNATAP -0.336209414565688 1.21874645332961e-07 NA NA NA hsa-miR-15b-3p IGF1R 1.12898579025388 1.18050912917317e-14 0.118209603036572 0.482168850037006 mirMAP -0.198448695749043 2.64062076757912e-07 NA NA NA hsa-miR-15b-5p IGF1R 0.788659356634696 1.13678211092534e-11 0.118209603036572 0.482168850037006 miRNATAP -0.206027032960709 2.56310348083525e-05 NA NA NA hsa-miR-16-1-3p IGF1R 1.30142784811196 2.53343713753744e-29 0.118209603036572 0.482168850037006 mirMAP -0.14097038784495 0.00570795285012571 NA NA NA hsa-miR-181a-2-3p IGF1R -0.0718643470098321 0.422077954889554 0.118209603036572 0.482168850037006 MirTarget -0.360130400204588 2.19622508166106e-08 NA NA NA hsa-miR-181b-5p IGF1R 1.22749257795094 1.21998198830919e-27 0.118209603036572 0.482168850037006 miRNAWalker2_validate -0.314350444937727 1.52635622217289e-10 NA NA NA hsa-miR-185-5p IGF1R 0.482334746589337 3.68475879959415e-09 0.118209603036572 0.482168850037006 MirTarget -0.547639501693991 2.52288181739065e-15 NA NA NA hsa-miR-186-5p IGF1R -0.426987301460247 1.04129380549315e-05 0.118209603036572 0.482168850037006 mirMAP -0.3758467428144 1.76981090502528e-10 NA NA NA hsa-miR-197-3p IGF1R -0.526589453596294 3.61594599437195e-06 0.118209603036572 0.482168850037006 mirMAP -0.346888473706894 3.79661059911104e-12 NA NA NA hsa-miR-19a-3p IGF1R 0.817507952171848 3.76285090469625e-08 0.118209603036572 0.482168850037006 mirMAP -0.280968212416369 1.34023709702226e-13 NA NA NA hsa-miR-22-5p IGF1R 0.238328801322329 0.0377496760295305 0.118209603036572 0.482168850037006 miRNATAP -0.295682146919029 3.20081591538083e-09 NA NA NA hsa-miR-223-3p IGF1R -0.966406159689943 7.90612601654887e-12 0.118209603036572 0.482168850037006 miRNAWalker2_validate;miRTarBase;MirTarget -0.327294415285608 1.36068714681003e-16 NA NA NA hsa-miR-224-5p IGF1R -2.22249420723892 1.07787949391113e-18 0.118209603036572 0.482168850037006 mirMAP -0.262380884793216 2.0900858366331e-34 NA NA NA hsa-miR-27a-3p IGF1R 0.388496361379095 1.42373532086331e-05 0.118209603036572 0.482168850037006 mirMAP -0.421381250131072 3.92848078456193e-11 NA NA NA hsa-miR-27b-3p IGF1R -0.0796803270437447 0.410503155574393 0.118209603036572 0.482168850037006 mirMAP -0.178939647350548 0.00277834896804242 NA NA NA hsa-miR-28-3p IGF1R -0.815347900986328 3.29387468083828e-30 0.118209603036572 0.482168850037006 miRNATAP -0.227953787002667 0.0037140065116185 NA NA NA hsa-miR-29a-3p IGF1R -0.699539666833076 1.13935003139957e-13 0.118209603036572 0.482168850037006 mirMAP -0.170708697516285 0.00479364896792699 NA NA NA hsa-miR-30e-5p IGF1R 0.293491764699475 0.000372291967271834 0.118209603036572 0.482168850037006 mirMAP -0.273677390130313 9.34606129272947e-05 NA NA NA hsa-miR-320b IGF1R 0.412787504747938 0.00113457276903985 0.118209603036572 0.482168850037006 PITA;mirMAP;miRNATAP -0.128021905768405 0.00503727353000704 NA NA NA hsa-miR-3613-5p IGF1R 1.11067990676844 4.33424951743633e-21 0.118209603036572 0.482168850037006 mirMAP;miRNATAP -0.187739659554805 8.4057598129776e-05 NA NA NA hsa-miR-369-3p IGF1R 0.0880848299432269 0.48519534195924 0.118209603036572 0.482168850037006 PITA -0.197324616652377 1.69203394230354e-05 NA NA NA hsa-miR-374a-5p IGF1R -0.239709888246804 0.00309819667153627 0.118209603036572 0.482168850037006 mirMAP -0.462875503518205 6.29233435988137e-11 NA NA NA hsa-miR-374b-5p IGF1R -0.466781429946153 1.17238311619116e-08 0.118209603036572 0.482168850037006 mirMAP -0.38240327187586 4.43580587406014e-08 NA NA NA hsa-miR-378a-3p IGF1R -1.79882978380301 1.05005373951733e-38 0.118209603036572 0.482168850037006 miRTarBase;MirTarget;miRNATAP -0.410965170800831 1.52092274416319e-26 24119742;25427715;24412052 The insulin-like growth factor 1 receptor IGF1R was predicted as a potential target of miR-378 and luciferase reporter activity of IGF1R was significantly decreased in the HEK293 cells co-transfected with miR-378 by 41.8% vs the control cells P less than 0.01; miR-378 may suppress growth characteristics of HBV-related HCC by directly targeting the IGF1R 3'-UTR and inhibiting its protein expression;Interestingly members of the miR-378 family presented as a possible target the insulin-like growth factor receptor 1 IGF1R a key signalling molecule in RMS; MiR-378a-3p over-expression in an RMS-derived cell line suppressed IGF1R expression and affected phosphorylated-Akt protein levels;Furthermore miR-378a-3p over-expression or down-regulation could inhibit or enhance insulin-like growth factor 1 receptor IGF1R expression in CRC cells; There was a significantly negative correlation between IGF1R protein expression and miR-378a-3p expression in CRC tissues ;; liver cancer;sarcoma;colorectal cancer hsa-miR-378a-5p IGF1R -2.43173551693523 1.44932837789175e-68 0.118209603036572 0.482168850037006 mirMAP -0.366238005092466 5.06012393985433e-23 24119742;25427715 The insulin-like growth factor 1 receptor IGF1R was predicted as a potential target of miR-378 and luciferase reporter activity of IGF1R was significantly decreased in the HEK293 cells co-transfected with miR-378 by 41.8% vs the control cells P less than 0.01; miR-378 may suppress growth characteristics of HBV-related HCC by directly targeting the IGF1R 3'-UTR and inhibiting its protein expression;Interestingly members of the miR-378 family presented as a possible target the insulin-like growth factor receptor 1 IGF1R a key signalling molecule in RMS ; liver cancer;sarcoma hsa-miR-378c IGF1R -1.79697571999492 1.07363220292403e-41 0.118209603036572 0.482168850037006 MirTarget;miRNATAP -0.372524048647036 2.92916006594659e-20 NA NA NA hsa-miR-452-5p IGF1R -2.48486455572568 1.24430987974325e-33 0.118209603036572 0.482168850037006 mirMAP -0.344176900884078 2.14757949011712e-41 NA NA NA hsa-miR-455-5p IGF1R 0.284641224662773 0.0357572339594277 0.118209603036572 0.482168850037006 miRNATAP -0.37596874913464 1.77232473048871e-19 NA NA NA hsa-miR-484 IGF1R 0.349444060004737 0.00154491249625173 0.118209603036572 0.482168850037006 miRNAWalker2_validate -0.138614288605464 0.00800305817129001 NA NA NA hsa-miR-495-3p IGF1R -1.67685437612894 1.70634429769052e-34 0.118209603036572 0.482168850037006 mirMAP -0.128987170041706 0.00143461494133436 NA NA NA hsa-miR-500a-3p IGF1R 0.139523673241448 0.185528510741158 0.118209603036572 0.482168850037006 mirMAP -0.595319934777455 3.03106324107414e-29 NA NA NA hsa-miR-532-5p IGF1R 0.0386976222860582 0.682018574136515 0.118209603036572 0.482168850037006 miRNATAP -0.573015490854942 1.08093916899163e-21 NA NA NA hsa-miR-582-3p IGF1R 0.126152488945932 0.333088461939587 0.118209603036572 0.482168850037006 PITA -0.114761029626503 0.00960538341063417 NA NA NA hsa-miR-671-5p IGF1R 1.55248047544015 7.7377784125886e-33 0.118209603036572 0.482168850037006 mirMAP -0.205843673722281 1.93098107239342e-06 NA NA NA hsa-miR-92a-3p IGF1R -0.342420481092807 0.000732275969501111 0.118209603036572 0.482168850037006 miRNAWalker2_validate -0.292401751465085 2.39617627316904e-07 NA NA NA hsa-miR-98-5p IGF1R 0.656336869298973 9.03113188590466e-15 0.118209603036572 0.482168850037006 MirTarget -0.35419321737761 1.24116459021004e-07 NA NA NA hsa-miR-130b-3p IGF2 1.38122163823846 1.00932179737836e-26 -1.80707502143834 9.64580354953106e-21 mirMAP -0.502522463738945 8.75042014015627e-24 NA NA NA hsa-miR-141-3p IGF2 2.99777540204168 1.26370498493024e-78 -1.80707502143834 9.64580354953106e-21 MirTarget;TargetScan -0.526450181465424 1.71357013794482e-47 NA NA NA hsa-miR-148a-3p IGF2 0.706969798912638 5.52538085480485e-09 -1.80707502143834 9.64580354953106e-21 mirMAP -0.483254422697307 7.93109854927416e-19 NA NA NA hsa-miR-148b-3p IGF2 1.0552576751879 1.6679285705231e-29 -1.80707502143834 9.64580354953106e-21 mirMAP -0.894988716311036 1.1779030441463e-40 NA NA NA hsa-miR-192-5p IGF2 1.33366224451777 9.5116159697107e-29 -1.80707502143834 9.64580354953106e-21 mirMAP -0.57061300154468 7.28513686071406e-27 NA NA NA hsa-miR-193a-3p IGF2 0.310617999804917 0.00477640270974957 -1.80707502143834 9.64580354953106e-21 mirMAP -0.376228277380693 8.52000035402321e-10 NA NA NA hsa-miR-19a-3p IGF2 0.817507952171848 3.76285090469625e-08 -1.80707502143834 9.64580354953106e-21 mirMAP -0.500967626901559 2.82727392231526e-30 NA NA NA hsa-miR-19b-3p IGF2 0.139124391010031 0.255658553872934 -1.80707502143834 9.64580354953106e-21 mirMAP -0.523445616011191 5.62894202989734e-22 NA NA NA hsa-miR-200a-3p IGF2 2.55218480823434 2.96135446816238e-51 -1.80707502143834 9.64580354953106e-21 MirTarget -0.43049563526396 2.80291025727271e-32 NA NA NA hsa-miR-210-3p IGF2 2.38367203491819 3.73261999106754e-22 -1.80707502143834 9.64580354953106e-21 MirTarget -0.334005796953098 7.04725823217349e-39 NA NA NA hsa-miR-301a-3p IGF2 1.32170168375632 2.12280368957514e-15 -1.80707502143834 9.64580354953106e-21 mirMAP -0.43546719532613 6.60141842803586e-29 NA NA NA hsa-miR-324-3p IGF2 0.404877731996915 0.000340036423920834 -1.80707502143834 9.64580354953106e-21 MirTarget;miRNATAP -0.609379587853179 1.74562567432832e-25 NA NA NA hsa-miR-339-5p IGF2 -0.211548290465418 0.0685885445822389 -1.80707502143834 9.64580354953106e-21 mirMAP -0.293532322036892 4.938517053805e-07 NA NA NA hsa-miR-342-5p IGF2 1.49554286219821 1.56221300255782e-22 -1.80707502143834 9.64580354953106e-21 MirTarget;mirMAP;miRNATAP -0.241664732659148 1.81332355609559e-08 NA NA NA hsa-miR-34a-5p IGF2 0.129201805242403 0.133261654068224 -1.80707502143834 9.64580354953106e-21 mirMAP -0.242484753846277 0.00229621728593982 NA NA NA hsa-miR-361-3p IGF2 -0.0370446538644886 0.624902392829872 -1.80707502143834 9.64580354953106e-21 mirMAP -0.472218454531822 1.62134013482131e-07 NA NA NA hsa-miR-454-3p IGF2 1.28177672191095 6.84024979484587e-21 -1.80707502143834 9.64580354953106e-21 mirMAP -0.493933025372171 2.31960198985041e-25 NA NA NA hsa-miR-455-5p IGF2 0.284641224662773 0.0357572339594277 -1.80707502143834 9.64580354953106e-21 mirMAP -0.183111096567841 0.000260407841126318 NA NA NA hsa-miR-484 IGF2 0.349444060004737 0.00154491249625173 -1.80707502143834 9.64580354953106e-21 miRNATAP -0.525568576532267 3.49127911615264e-18 NA NA NA hsa-miR-628-5p IGF2 0.107482129280898 0.569748271582925 -1.80707502143834 9.64580354953106e-21 mirMAP -0.173521078128283 1.24958032458701e-06 NA NA NA hsa-miR-744-3p IGF2 -0.00965764791535539 0.955204760097016 -1.80707502143834 9.64580354953106e-21 mirMAP -0.316277215330137 4.25874716426272e-16 NA NA NA hsa-miR-744-5p IGF2 0.114590984077262 0.312916425462546 -1.80707502143834 9.64580354953106e-21 MirTarget -0.461331772327901 6.20346139911665e-15 NA NA NA hsa-miR-9-5p IGF2 0.734772328078424 0.00261982843636383 -1.80707502143834 9.64580354953106e-21 mirMAP -0.218646593239723 1.15371975303189e-15 NA NA NA hsa-miR-93-5p IGF2 1.01788600209314 1.24312921618126e-21 -1.80707502143834 9.64580354953106e-21 miRNAWalker2_validate -0.643440034437039 2.08793079509927e-26 NA NA NA hsa-miR-146b-5p IKBKB 0.631041661167954 6.20366593452965e-08 0.273066826249678 0.00234033685126725 miRanda -0.152908526927625 4.45729055656516e-09 NA NA NA hsa-miR-151a-3p IKBKB 0.3530403796941 3.45015092968892e-05 0.273066826249678 0.00234033685126725 miRNAWalker2_validate -0.109349947297392 0.0025250144404732 NA NA NA hsa-miR-28-5p IKBKB -0.14267073663385 0.0497619246260571 0.273066826249678 0.00234033685126725 miRanda -0.249159305195741 4.36298401628208e-09 NA NA NA hsa-miR-324-5p IKBKB 0.211552647440779 0.139408130311654 0.273066826249678 0.00234033685126725 miRanda -0.118429787871439 3.07201825932054e-08 NA NA NA hsa-miR-330-5p IKBKB 0.626063225953928 3.42215028606779e-09 0.273066826249678 0.00234033685126725 miRanda -0.115452535316074 6.19261343388698e-05 NA NA NA hsa-miR-590-3p IKBKB 1.27438512890514 1.03164723481469e-23 0.273066826249678 0.00234033685126725 miRanda;mirMAP -0.100127823485223 4.7662908944255e-05 NA NA NA hsa-miR-125a-5p IKBKG -0.635694700253268 3.6703235721638e-11 0.490716396303581 1.1441359682473e-11 miRanda -0.228007302003838 2.38576015741085e-19 NA NA NA hsa-miR-140-5p IKBKG -0.755971041589807 9.68060922934542e-14 0.490716396303581 1.1441359682473e-11 miRanda -0.125852887257061 2.14634764656029e-07 NA NA NA hsa-miR-3607-3p IKBKG 0.00697875745616461 0.972073275173643 0.490716396303581 1.1441359682473e-11 mirMAP -0.123479124494569 5.75170407705992e-24 NA NA NA hsa-miR-3065-3p IL2RA 1.62787885002468 6.81477830005026e-19 0.552215139772291 0.00781659262634694 MirTarget -0.178827340978585 2.28054297181163e-06 NA NA NA hsa-miR-375 IL2RA 2.09893673049766 1.68351780396203e-16 0.552215139772291 0.00781659262634694 miRanda -0.226533685168416 3.8944695458836e-17 NA NA NA hsa-miR-125a-5p IL2RB -0.635694700253268 3.6703235721638e-11 0.409418481998368 0.0157972444641947 miRanda -0.29694183193025 7.00572007406091e-07 NA NA NA hsa-miR-30b-3p IL2RB -0.536462824497982 2.41814659272548e-05 0.409418481998368 0.0157972444641947 mirMAP -0.120917386166261 0.00852832449925365 NA NA NA hsa-miR-491-5p IL2RG -1.04307210952796 1.14586501470398e-15 0.527564404518184 0.0019177001745873 miRanda -0.177810126037921 6.02916563584062e-05 NA NA NA hsa-miR-331-3p IL4R 0.74441480542886 1.17071235477799e-07 -0.29952988564671 0.00152305772318984 miRNAWalker2_validate -0.164261893401686 3.92837965967983e-13 NA NA NA hsa-miR-339-5p IL4R -0.211548290465418 0.0685885445822389 -0.29952988564671 0.00152305772318984 miRanda -0.114416843350994 4.14803636170354e-05 NA NA NA hsa-miR-491-5p IL4R -1.04307210952796 1.14586501470398e-15 -0.29952988564671 0.00152305772318984 miRanda -0.104586865246844 2.11908180578236e-05 NA NA NA hsa-miR-107 IL6 0.678998026350662 2.56224148930538e-16 -3.07751927232311 9.9131162785608e-32 miRanda -0.335195787804263 0.00267549740903922 NA NA NA hsa-miR-429 IL6 2.84108892259147 3.88520896426515e-64 -3.07751927232311 9.9131162785608e-32 miRanda -0.424185146928772 6.81793191441461e-17 NA NA NA hsa-let-7e-5p IL6R 0.261471269338985 0.00191077095550257 -1.4959681212526 3.98690474593025e-23 miRNATAP -0.17507204538523 0.00585557898341552 NA NA NA hsa-miR-106b-5p IL6R 1.22477789913175 2.31787213143326e-32 -1.4959681212526 3.98690474593025e-23 mirMAP -0.307640134694259 3.99966327961051e-10 NA NA NA hsa-miR-130b-3p IL6R 1.38122163823846 1.00932179737836e-26 -1.4959681212526 3.98690474593025e-23 mirMAP -0.127525369485403 0.00150827294845077 NA NA NA hsa-miR-141-3p IL6R 2.99777540204168 1.26370498493024e-78 -1.4959681212526 3.98690474593025e-23 MirTarget -0.253098258336402 1.27382588367647e-17 NA NA NA hsa-miR-141-5p IL6R 2.38411349683216 4.56310582620282e-58 -1.4959681212526 3.98690474593025e-23 MirTarget -0.211774282012291 1.43748300605942e-10 NA NA NA hsa-miR-182-5p IL6R 2.36408084103818 2.60011457600281e-72 -1.4959681212526 3.98690474593025e-23 mirMAP -0.379208971544044 4.41744748962182e-26 NA NA NA hsa-miR-193a-3p IL6R 0.310617999804917 0.00477640270974957 -1.4959681212526 3.98690474593025e-23 mirMAP -0.186229101097756 0.00011695816491136 NA NA NA hsa-miR-193b-3p IL6R -0.0550374855074773 0.65382485898551 -1.4959681212526 3.98690474593025e-23 mirMAP -0.154861771649225 0.000363645984194793 NA NA NA hsa-miR-200a-3p IL6R 2.55218480823434 2.96135446816238e-51 -1.4959681212526 3.98690474593025e-23 MirTarget -0.235380598297946 6.33404340334906e-16 NA NA NA hsa-miR-20a-5p IL6R 0.44979911120222 0.000602386964327228 -1.4959681212526 3.98690474593025e-23 mirMAP -0.125418245247442 0.00197382511035134 NA NA NA hsa-miR-21-5p IL6R 2.32007501846389 3.08394781855554e-137 -1.4959681212526 3.98690474593025e-23 MirTarget;miRNATAP -0.329109136402319 6.05386734548003e-12 NA NA NA hsa-miR-301a-3p IL6R 1.32170168375632 2.12280368957514e-15 -1.4959681212526 3.98690474593025e-23 mirMAP -0.153584802129653 1.05970398736436e-06 NA NA NA hsa-miR-340-5p IL6R 1.0857159627596 4.94471062546756e-24 -1.4959681212526 3.98690474593025e-23 mirMAP -0.193028628130182 6.27919851615181e-05 NA NA NA hsa-miR-454-3p IL6R 1.28177672191095 6.84024979484587e-21 -1.4959681212526 3.98690474593025e-23 mirMAP -0.219027759212429 8.1279201972906e-09 NA NA NA hsa-miR-93-5p IL6R 1.01788600209314 1.24312921618126e-21 -1.4959681212526 3.98690474593025e-23 mirMAP -0.333750047512298 4.8028519401738e-12 NA NA NA hsa-miR-98-5p IL6R 0.656336869298973 9.03113188590466e-15 -1.4959681212526 3.98690474593025e-23 miRNAWalker2_validate -0.214105458008283 0.00057577278896208 NA NA NA hsa-miR-192-5p IL7 1.33366224451777 9.5116159697107e-29 -1.15623141298675 2.89787466175942e-14 miRNAWalker2_validate -0.140015624448661 0.00104644268433873 NA NA NA hsa-miR-429 IL7 2.84108892259147 3.88520896426515e-64 -1.15623141298675 2.89787466175942e-14 miRanda -0.191776055201327 3.56427683831472e-11 NA NA NA hsa-miR-181a-5p IL7R 0.635863607630183 6.72486918157744e-11 -0.94953189474671 0.000239240351308242 mirMAP -0.278666463769429 0.00209346116355377 NA NA NA hsa-miR-181b-5p IL7R 1.22749257795094 1.21998198830919e-27 -0.94953189474671 0.000239240351308242 mirMAP -0.268069156007438 0.000450229999141897 NA NA NA hsa-miR-181c-5p IL7R 0.574847839298605 4.51287502066478e-07 -0.94953189474671 0.000239240351308242 mirMAP -0.201331441874885 0.00962448518990064 NA NA NA hsa-let-7i-5p INSR -0.333419326258177 3.56960810786801e-08 -0.27492376201698 0.000536820623484817 miRNATAP -0.193128410769688 2.01434335345171e-05 NA NA NA hsa-miR-140-3p INSR -1.25844395244141 5.29222651039892e-33 -0.27492376201698 0.000536820623484817 mirMAP -0.110695842846824 8.67829836185724e-06 NA NA NA hsa-miR-15a-5p INSR 0.812445324157528 4.50263417099822e-20 -0.27492376201698 0.000536820623484817 miRNATAP -0.150758131062672 5.46108942040012e-07 NA NA NA hsa-miR-181a-5p INSR 0.635863607630183 6.72486918157744e-11 -0.27492376201698 0.000536820623484817 mirMAP -0.117022056564807 2.4043081879531e-05 NA NA NA hsa-miR-181b-5p INSR 1.22749257795094 1.21998198830919e-27 -0.27492376201698 0.000536820623484817 mirMAP -0.117276360210961 5.03874837628364e-07 NA NA NA hsa-miR-21-5p INSR 2.32007501846389 3.08394781855554e-137 -0.27492376201698 0.000536820623484817 mirMAP -0.169950201921848 3.994162980573e-12 24804226;27220494;21618027 Downregulation of miR-21 in radioresistant NSCLC A549 cells inhibited the colony-forming ability and proliferation of A549 cells after IR; Moreover silencing miR-21 enhanced apoptosis of A549 cells induced by IR accompanied by decreased phosphorylated-Akt protein level; However PI3K activator IGF-1 reversed suppression of phosphorylated-Akt protein level and promotion of apoptosis of A549 cells after IR caused by miR-21 knockdown; Silencing miR-21 in radioresistant NSCLC A549 cells sensitized them to IR by inhibiting cell proliferation and enhancing cell apoptosis through inhibition of PI3K/Akt signaling pathway;In addition we also demonstrated that miR-21 confers decreased autophagy in cervical cancer cells after IR via the Akt-mTOR signaling pathway;In this study we found that miR-21 expression was upregulated in response to ionizing radiation IR in U251 cells which suggested that miR-21 could be involved in the response of U251 cells to radiation; We showed that a miR-21 inhibitor enhanced IR-induced glioblastoma cell growth arrest and increased the level of apoptosis which was probably caused by abrogation of the G2-M arrest induced by IR ;;drug resistance lung squamous cell cancer;cervical and endocervical cancer;glioblastoma hsa-miR-361-5p INSR -0.0996867547900457 0.095428250126326 -0.27492376201698 0.000536820623484817 miRanda -0.254039592402768 3.53889340380226e-08 NA NA NA hsa-miR-374a-3p INSR 0.34122393953022 2.28714105382856e-05 -0.27492376201698 0.000536820623484817 mirMAP -0.134498034427715 7.19695391572516e-05 NA NA NA hsa-miR-128-3p IRS1 0.90296715955238 9.925204823863e-23 -1.09359999788168 5.90327201022502e-13 MirTarget -0.516101436793944 4.76442658080806e-21 26352220 In addition insulin receptor substrate 1 IRS1 a key mediator in oncogenic insulin-like growth factor IGF signaling was confirmed as a direct target of miR-128 by a luciferase reporter assay; Western blot analysis indicated that the overexpression of miR-128 significantly downregulated IRS1 expression and its downstream Akt signaling in CRC cells; Moreover miR-128 was negatively associated with IRS1 in CRC tissues compared to adjacent non-tumor tissues; Taken together these data suggested that miR-128 serves as a tumor suppressor and blocks CRC growth and metastasis by targeting IRS1 metastasis colorectal cancer hsa-miR-142-5p IRS1 1.16509303143593 1.96641162209073e-13 -1.09359999788168 5.90327201022502e-13 MirTarget;PITA -0.278677636402086 4.7517650901109e-18 NA NA NA hsa-miR-148a-3p IRS1 0.706969798912638 5.52538085480485e-09 -1.09359999788168 5.90327201022502e-13 miRNAWalker2_validate;miRTarBase -0.30022382292007 2.08482327044964e-12 NA NA NA hsa-miR-16-1-3p IRS1 1.30142784811196 2.53343713753744e-29 -1.09359999788168 5.90327201022502e-13 MirTarget -0.264746652710615 1.15304027473658e-08 NA NA NA hsa-miR-17-3p IRS1 0.721439431208839 7.95900548819742e-10 -1.09359999788168 5.90327201022502e-13 MirTarget -0.442821975034471 1.85644060475937e-24 NA NA NA hsa-miR-183-5p IRS1 2.95681814393872 1.09965777344285e-92 -1.09359999788168 5.90327201022502e-13 MirTarget;miRNATAP -0.199350251372208 5.75374286443932e-10 NA NA NA hsa-miR-200c-3p IRS1 2.07038651806183 6.84613386286599e-51 -1.09359999788168 5.90327201022502e-13 miRNATAP -0.176788257679081 7.61635995828395e-07 NA NA NA hsa-miR-203a-3p IRS1 1.67084394806148 6.06522273352132e-14 -1.09359999788168 5.90327201022502e-13 MirTarget -0.14369637894903 5.08437569345363e-10 NA NA NA hsa-miR-27a-3p IRS1 0.388496361379095 1.42373532086331e-05 -1.09359999788168 5.90327201022502e-13 MirTarget;miRNATAP -0.394727485317572 1.40332530772436e-11 NA NA NA hsa-miR-30e-5p IRS1 0.293491764699475 0.000372291967271834 -1.09359999788168 5.90327201022502e-13 MirTarget -0.363121554325956 1.30521965586155e-08 NA NA NA hsa-miR-33a-5p IRS1 1.35185127289399 9.80509199125785e-12 -1.09359999788168 5.90327201022502e-13 MirTarget -0.199427816231483 4.08280406713901e-14 NA NA NA hsa-miR-429 IRS1 2.84108892259147 3.88520896426515e-64 -1.09359999788168 5.90327201022502e-13 miRanda;miRNATAP -0.162361096724304 2.09525237425462e-08 NA NA NA hsa-miR-590-3p IRS1 1.27438512890514 1.03164723481469e-23 -1.09359999788168 5.90327201022502e-13 PITA;miRanda;miRNATAP -0.428943757952659 1.67201957493221e-25 NA NA NA hsa-miR-660-5p IRS1 0.406910761707485 0.000156175371762464 -1.09359999788168 5.90327201022502e-13 MirTarget;miRNATAP -0.447265712978181 1.05582114595633e-20 NA NA NA hsa-miR-96-5p IRS1 3.24596558166606 1.92296159041707e-98 -1.09359999788168 5.90327201022502e-13 MirTarget;TargetScan;miRNATAP -0.143349063007378 2.27407069359301e-06 21993544 hsa miR 96 up regulates MAP4K1 and IRS1 and may function as a promising diagnostic marker in human bladder urothelial carcinomas; Altogether these results suggest that hsa-miR-96 may affect the growth of bladder cancer cells by up-regulating IRS1 and MAP4K1 levels functioning as a promising diagnostic marker in human bladder urothelial carcinomas bladder cancer hsa-miR-107 ITGA1 0.678998026350662 2.56224148930538e-16 -1.29512393000353 7.98953749577455e-27 miRanda -0.360710818395196 7.96050052702476e-13 NA NA NA hsa-miR-141-5p ITGA1 2.38411349683216 4.56310582620282e-58 -1.29512393000353 7.98953749577455e-27 MirTarget -0.256930514466659 7.45874120468767e-23 NA NA NA hsa-miR-181a-5p ITGA1 0.635863607630183 6.72486918157744e-11 -1.29512393000353 7.98953749577455e-27 mirMAP -0.274560290949497 2.03207603080682e-10 NA NA NA hsa-miR-181b-5p ITGA1 1.22749257795094 1.21998198830919e-27 -1.29512393000353 7.98953749577455e-27 mirMAP -0.290785239040699 9.43497682095055e-16 NA NA NA hsa-miR-181c-5p ITGA1 0.574847839298605 4.51287502066478e-07 -1.29512393000353 7.98953749577455e-27 mirMAP -0.333783425235148 7.16688054712579e-20 NA NA NA hsa-miR-200a-5p ITGA1 2.58844508672328 1.79650654234569e-56 -1.29512393000353 7.98953749577455e-27 mirMAP -0.217378046124112 7.48753974744995e-20 NA NA NA hsa-miR-200b-3p ITGA1 2.11744072703209 1.66710572104191e-43 -1.29512393000353 7.98953749577455e-27 MirTarget -0.315620670948116 1.43489959453689e-35 NA NA NA hsa-miR-200b-5p ITGA1 1.43091697052145 2.07975242866479e-22 -1.29512393000353 7.98953749577455e-27 mirMAP -0.178425171236032 6.15339396903684e-10 NA NA NA hsa-miR-200c-3p ITGA1 2.07038651806183 6.84613386286599e-51 -1.29512393000353 7.98953749577455e-27 MirTarget -0.346757618979074 1.67421615105689e-35 NA NA NA hsa-miR-21-5p ITGA1 2.32007501846389 3.08394781855554e-137 -1.29512393000353 7.98953749577455e-27 mirMAP -0.323092029358791 2.56001275037339e-17 NA NA NA hsa-miR-30d-3p ITGA1 0.264544633563642 0.00755023189129747 -1.29512393000353 7.98953749577455e-27 MirTarget -0.267396879999101 4.87505152750255e-10 NA NA NA hsa-miR-320a ITGA1 -0.287921276129346 0.00141693637596294 -1.29512393000353 7.98953749577455e-27 miRanda -0.136353089337455 0.00415993436420383 NA NA NA hsa-miR-320b ITGA1 0.412787504747938 0.00113457276903985 -1.29512393000353 7.98953749577455e-27 miRanda -0.108164678876596 0.0014011617009071 NA NA NA hsa-miR-330-3p ITGA1 0.432728362037032 0.00041335506960812 -1.29512393000353 7.98953749577455e-27 mirMAP -0.117582550167345 0.000872333477704689 NA NA NA hsa-miR-361-5p ITGA1 -0.0996867547900457 0.095428250126326 -1.29512393000353 7.98953749577455e-27 miRanda -0.450747532254013 4.08750278829306e-10 NA NA NA hsa-miR-429 ITGA1 2.84108892259147 3.88520896426515e-64 -1.29512393000353 7.98953749577455e-27 MirTarget;miRanda -0.275342516757783 6.9727437361557e-34 NA NA NA hsa-miR-590-3p ITGA1 1.27438512890514 1.03164723481469e-23 -1.29512393000353 7.98953749577455e-27 miRanda;mirMAP -0.173311593056365 3.75056986535531e-07 NA NA NA hsa-let-7g-3p ITGA10 0.5854327663695 0.000371711766083627 -1.01261887152059 1.26964937815038e-09 MirTarget;miRNATAP -0.25022145572231 4.44098506761892e-13 NA NA NA hsa-miR-107 ITGA10 0.678998026350662 2.56224148930538e-16 -1.01261887152059 1.26964937815038e-09 miRanda -0.293391742370594 1.93368760823932e-05 NA NA NA hsa-miR-15a-5p ITGA10 0.812445324157528 4.50263417099822e-20 -1.01261887152059 1.26964937815038e-09 MirTarget -0.256551494125704 5.9773637761757e-05 NA NA NA hsa-miR-15b-5p ITGA10 0.788659356634696 1.13678211092534e-11 -1.01261887152059 1.26964937815038e-09 MirTarget -0.288455114499232 3.43378293337261e-09 NA NA NA hsa-miR-16-5p ITGA10 0.46835991343556 1.91125356547776e-06 -1.01261887152059 1.26964937815038e-09 MirTarget -0.519694834748005 1.35744457913283e-19 NA NA NA hsa-miR-22-3p ITGA10 -0.505819481364718 1.90494905330622e-13 -1.01261887152059 1.26964937815038e-09 MirTarget -0.237891647892153 0.00448546970714205 NA NA NA hsa-miR-324-5p ITGA10 0.211552647440779 0.139408130311654 -1.01261887152059 1.26964937815038e-09 miRanda;miRNATAP -0.268423674756526 1.8234381950223e-11 NA NA NA hsa-miR-338-3p ITGA10 0.0799903526884966 0.575137668326679 -1.01261887152059 1.26964937815038e-09 miRanda -0.108936063468032 0.0072047591779604 NA NA NA hsa-miR-429 ITGA10 2.84108892259147 3.88520896426515e-64 -1.01261887152059 1.26964937815038e-09 miRanda;miRNATAP -0.157135433897949 7.48029071890922e-07 NA NA NA hsa-miR-484 ITGA10 0.349444060004737 0.00154491249625173 -1.01261887152059 1.26964937815038e-09 miRNATAP -0.490022368221224 8.89098931625336e-22 NA NA NA hsa-miR-708-5p ITGA10 0.638386255192461 1.19735143565146e-06 -1.01261887152059 1.26964937815038e-09 MirTarget -0.242074924084671 2.48579510885826e-08 NA NA NA hsa-miR-29a-3p ITGA11 -0.699539666833076 1.13935003139957e-13 0.58367350429548 0.000556819748855291 miRNAWalker2_validate;miRTarBase -0.365504198702639 1.56675559117404e-09 NA NA NA hsa-miR-532-5p ITGA11 0.0386976222860582 0.682018574136515 0.58367350429548 0.000556819748855291 miRNATAP -0.223237654028104 0.000304389439139631 NA NA NA hsa-miR-103a-3p ITGA2 0.610673390034345 1.63113920514646e-12 -0.578073454534045 0.000245362336210971 miRNAWalker2_validate;MirTarget;miRNATAP -0.518553557094713 2.0689176307065e-17 NA NA NA hsa-miR-107 ITGA2 0.678998026350662 2.56224148930538e-16 -0.578073454534045 0.000245362336210971 MirTarget;miRanda;miRNATAP -0.578298559534823 5.92643308700432e-20 NA NA NA hsa-miR-130b-3p ITGA2 1.38122163823846 1.00932179737836e-26 -0.578073454534045 0.000245362336210971 mirMAP -0.290892177898957 6.18833747120119e-13 NA NA NA hsa-miR-15a-5p ITGA2 0.812445324157528 4.50263417099822e-20 -0.578073454534045 0.000245362336210971 MirTarget;miRNATAP -0.571710856591226 1.80311916315964e-22 NA NA NA hsa-miR-15b-5p ITGA2 0.788659356634696 1.13678211092534e-11 -0.578073454534045 0.000245362336210971 MirTarget;miRNATAP -0.380913533105479 4.41835184495374e-17 NA NA NA hsa-miR-16-5p ITGA2 0.46835991343556 1.91125356547776e-06 -0.578073454534045 0.000245362336210971 miRNAWalker2_validate;MirTarget;miRNATAP -0.242009742873415 1.02794727566796e-05 NA NA NA hsa-miR-181a-5p ITGA2 0.635863607630183 6.72486918157744e-11 -0.578073454534045 0.000245362336210971 MirTarget -0.205880789895555 0.000189169212142989 NA NA NA hsa-miR-181b-5p ITGA2 1.22749257795094 1.21998198830919e-27 -0.578073454534045 0.000245362336210971 MirTarget -0.22966433200823 7.49310977501828e-07 NA NA NA hsa-miR-185-5p ITGA2 0.482334746589337 3.68475879959415e-09 -0.578073454534045 0.000245362336210971 miRNATAP -0.711126708939395 1.04319804229734e-28 NA NA NA hsa-miR-18a-5p ITGA2 0.969066793715868 1.69069796425892e-08 -0.578073454534045 0.000245362336210971 MirTarget -0.277288585922338 1.4587093955565e-19 NA NA NA hsa-miR-19a-3p ITGA2 0.817507952171848 3.76285090469625e-08 -0.578073454534045 0.000245362336210971 MirTarget -0.183101395943965 3.69579697317755e-07 NA NA NA hsa-miR-19b-3p ITGA2 0.139124391010031 0.255658553872934 -0.578073454534045 0.000245362336210971 MirTarget -0.158712773707016 0.00034384673625258 NA NA NA hsa-miR-27a-3p ITGA2 0.388496361379095 1.42373532086331e-05 -0.578073454534045 0.000245362336210971 miRNATAP -0.191806915756419 0.00155051571375338 NA NA NA hsa-miR-301a-3p ITGA2 1.32170168375632 2.12280368957514e-15 -0.578073454534045 0.000245362336210971 mirMAP -0.166685535121401 1.92153514631417e-07 NA NA NA hsa-miR-30e-5p ITGA2 0.293491764699475 0.000372291967271834 -0.578073454534045 0.000245362336210971 mirMAP -0.174823441914395 0.00813656907618175 NA NA NA hsa-miR-324-5p ITGA2 0.211552647440779 0.139408130311654 -0.578073454534045 0.000245362336210971 miRanda -0.136513885303923 0.000310229032135042 NA NA NA hsa-miR-330-5p ITGA2 0.626063225953928 3.42215028606779e-09 -0.578073454534045 0.000245362336210971 PITA -0.136903008445456 0.0071300294329751 NA NA NA hsa-miR-340-5p ITGA2 1.0857159627596 4.94471062546756e-24 -0.578073454534045 0.000245362336210971 mirMAP -0.398174029476505 1.84496145385471e-16 NA NA NA hsa-miR-374a-5p ITGA2 -0.239709888246804 0.00309819667153627 -0.578073454534045 0.000245362336210971 MirTarget -0.315553562871544 2.50089061582709e-06 NA NA NA hsa-miR-374b-5p ITGA2 -0.466781429946153 1.17238311619116e-08 -0.578073454534045 0.000245362336210971 MirTarget -0.273064428324435 3.50223913512016e-05 NA NA NA hsa-miR-454-3p ITGA2 1.28177672191095 6.84024979484587e-21 -0.578073454534045 0.000245362336210971 mirMAP -0.188214261131704 1.22159793880003e-06 NA NA NA hsa-miR-532-5p ITGA2 0.0386976222860582 0.682018574136515 -0.578073454534045 0.000245362336210971 MirTarget -0.308713851602447 7.4391196813151e-08 NA NA NA hsa-miR-590-3p ITGA2 1.27438512890514 1.03164723481469e-23 -0.578073454534045 0.000245362336210971 MirTarget;miRanda;mirMAP -0.266166940907233 6.42250404215377e-10 NA NA NA hsa-miR-590-5p ITGA2 0.746515607868274 8.51300479640125e-09 -0.578073454534045 0.000245362336210971 miRanda -0.245731794950449 2.43759428268426e-09 NA NA NA hsa-miR-93-3p ITGA2 0.627759242044891 3.62527588304617e-06 -0.578073454534045 0.000245362336210971 MirTarget -0.122698897518948 0.00205245997033807 NA NA NA hsa-let-7a-3p ITGA3 0.466622567891891 3.70886257943079e-07 -0.299252352501844 0.0107499356635658 MirTarget;miRNATAP -0.124180274882071 0.00457163803013909 NA NA NA hsa-let-7f-1-3p ITGA3 0.37532726250321 0.00156021688753682 -0.299252352501844 0.0107499356635658 MirTarget -0.181412784841988 1.10062775439581e-07 NA NA NA hsa-miR-181a-5p ITGA3 0.635863607630183 6.72486918157744e-11 -0.299252352501844 0.0107499356635658 miRNATAP -0.118738593286317 0.00379092899252998 NA NA NA hsa-miR-181b-5p ITGA3 1.22749257795094 1.21998198830919e-27 -0.299252352501844 0.0107499356635658 miRNATAP -0.141676587277426 4.09633853169439e-05 NA NA NA hsa-miR-185-3p ITGA3 0.86591374114446 7.8790769678542e-10 -0.299252352501844 0.0107499356635658 MirTarget -0.201653772384404 1.26648850468307e-12 NA NA NA hsa-miR-186-5p ITGA3 -0.426987301460247 1.04129380549315e-05 -0.299252352501844 0.0107499356635658 miRNATAP -0.371980442215603 5.44987847157337e-20 NA NA NA hsa-miR-222-5p ITGA3 -0.0126640010215462 0.929279244700619 -0.299252352501844 0.0107499356635658 MirTarget -0.142441867500999 4.38403893501516e-07 NA NA NA hsa-miR-24-3p ITGA3 -0.120123317289941 0.21432376743243 -0.299252352501844 0.0107499356635658 miRNATAP -0.270588468315339 6.28825332413991e-11 NA NA NA hsa-miR-28-5p ITGA3 -0.14267073663385 0.0497619246260571 -0.299252352501844 0.0107499356635658 miRanda -0.36126889309902 6.72512700126372e-11 NA NA NA hsa-miR-330-5p ITGA3 0.626063225953928 3.42215028606779e-09 -0.299252352501844 0.0107499356635658 miRanda -0.205713288606192 4.19688854611286e-08 NA NA NA hsa-miR-339-5p ITGA3 -0.211548290465418 0.0685885445822389 -0.299252352501844 0.0107499356635658 MirTarget;miRanda -0.109803723960993 0.00155802251212262 NA NA NA hsa-miR-455-5p ITGA3 0.284641224662773 0.0357572339594277 -0.299252352501844 0.0107499356635658 miRanda -0.182352642454681 6.04973316101048e-10 NA NA NA hsa-miR-30c-5p ITGA4 -0.393222674580066 8.06961465717306e-05 0.254180639033599 0.0455327600453091 miRNAWalker2_validate -0.196342383284604 6.55018570755671e-06 NA NA NA hsa-miR-148b-3p ITGA5 1.0552576751879 1.6679285705231e-29 0.258471542303731 0.00349408854059877 miRNAWalker2_validate -0.122356058603336 9.68208950992594e-05 27328731 Notably transendothelial migration in vitro and extravasation in vivo impaired by singly alternating miR-214 or miR-148b could be overridden by overexpression of ITGA5 or ALCAM in the same tumor cells melanoma hsa-miR-25-3p ITGA5 -0.00546790775985073 0.944061771389468 0.258471542303731 0.00349408854059877 miRNATAP -0.133075462333109 0.000708776724292318 NA NA NA hsa-miR-26a-5p ITGA5 -0.481180084102235 1.82368246174758e-10 0.258471542303731 0.00349408854059877 miRNATAP -0.131306000973994 0.0010672095572809 25537511 Furthermore we provide experimental evidence to confirm that ITGA5 is a bona fide target of miR-26a liver cancer hsa-miR-26b-5p ITGA5 -0.331527896896866 0.000551258576575092 0.258471542303731 0.00349408854059877 miRNATAP -0.104503299205837 0.000970956331959348 NA NA NA hsa-miR-30a-5p ITGA5 -0.392489994825279 0.00954894667761676 0.258471542303731 0.00349408854059877 miRNATAP -0.126371446171278 1.90284369217649e-10 NA NA NA hsa-miR-30c-5p ITGA5 -0.393222674580066 8.06961465717306e-05 0.258471542303731 0.00349408854059877 miRNATAP -0.222941024449013 1.1526773250089e-13 NA NA NA hsa-miR-30d-5p ITGA5 -0.247082219011045 0.00406717930063148 0.258471542303731 0.00349408854059877 miRNATAP -0.329430073488982 1.88892816446792e-21 NA NA NA hsa-miR-92a-3p ITGA5 -0.342420481092807 0.000732275969501111 0.258471542303731 0.00349408854059877 miRNAWalker2_validate;miRTarBase;miRNATAP -0.14127429191828 2.23941319844575e-06 23499550 The level of miR-92a expression was significantly inversely correlated with ITGA5 expression in various cancer cells; miR-92a overexpression reduced the luciferase activity of the ITGA5 3'-untranslated region suggesting that ITGA5 mRNA is a direct target of miR-92a ovarian cancer hsa-miR-15b-5p ITGA6 0.788659356634696 1.13678211092534e-11 -1.38118153822314 3.75754642654678e-26 miRNAWalker2_validate -0.139367755454889 0.000391425145386089 NA NA NA hsa-miR-32-5p ITGA6 1.38676603945192 2.29428299685976e-34 -1.38118153822314 3.75754642654678e-26 miRNAWalker2_validate -0.121079963666484 0.00196209410668541 NA NA NA hsa-miR-342-3p ITGA6 1.21944481975657 6.81914373001202e-19 -1.38118153822314 3.75754642654678e-26 miRanda -0.188358642682528 8.25802470227682e-09 NA NA NA hsa-miR-330-5p ITGA7 0.626063225953928 3.42215028606779e-09 -3.42938571679422 4.12756715511965e-78 miRanda -0.645663755348389 1.98188299206038e-24 NA NA NA hsa-miR-339-5p ITGA7 -0.211548290465418 0.0685885445822389 -3.42938571679422 4.12756715511965e-78 miRanda -0.154798310368845 0.00974568162043182 NA NA NA hsa-miR-615-3p ITGA7 0.659240580933614 0.000360214386152507 -3.42938571679422 4.12756715511965e-78 miRNAWalker2_validate -0.260625486838749 1.88695834226043e-12 NA NA NA hsa-miR-130b-3p ITGA8 1.38122163823846 1.00932179737836e-26 -1.33167258927312 3.90791960236807e-09 mirMAP -0.363531829192523 5.51069664420395e-10 NA NA NA hsa-miR-16-1-3p ITGA8 1.30142784811196 2.53343713753744e-29 -1.33167258927312 3.90791960236807e-09 mirMAP -0.488642240734853 9.10322187741045e-13 NA NA NA hsa-miR-183-5p ITGA8 2.95681814393872 1.09965777344285e-92 -1.33167258927312 3.90791960236807e-09 miRNAWalker2_validate -0.278281073234491 5.61678710347892e-09 NA NA NA hsa-miR-19b-1-5p ITGA8 0.647704962003062 3.07687667945597e-07 -1.33167258927312 3.90791960236807e-09 mirMAP -0.514203459470821 1.74155500290764e-17 NA NA NA hsa-miR-21-5p ITGA8 2.32007501846389 3.08394781855554e-137 -1.33167258927312 3.90791960236807e-09 mirMAP -0.342105492128526 1.37371548465133e-06 NA NA NA hsa-miR-301a-3p ITGA8 1.32170168375632 2.12280368957514e-15 -1.33167258927312 3.90791960236807e-09 mirMAP -0.36265747189838 2.22867286891805e-15 NA NA NA hsa-miR-30b-5p ITGA8 -0.46540603860146 2.4709940429591e-05 -1.33167258927312 3.90791960236807e-09 mirMAP -0.353958762443397 5.1030620941536e-07 NA NA NA hsa-miR-30c-5p ITGA8 -0.393222674580066 8.06961465717306e-05 -1.33167258927312 3.90791960236807e-09 mirMAP -0.408830465208232 1.63637571622999e-07 NA NA NA hsa-miR-30d-5p ITGA8 -0.247082219011045 0.00406717930063148 -1.33167258927312 3.90791960236807e-09 mirMAP -0.273845615801928 0.00275158961654194 NA NA NA hsa-miR-33a-5p ITGA8 1.35185127289399 9.80509199125785e-12 -1.33167258927312 3.90791960236807e-09 mirMAP -0.214427754837525 5.39256493186554e-08 NA NA NA hsa-miR-454-3p ITGA8 1.28177672191095 6.84024979484587e-21 -1.33167258927312 3.90791960236807e-09 mirMAP -0.40057249108339 5.53256423312264e-13 NA NA NA hsa-miR-590-5p ITGA8 0.746515607868274 8.51300479640125e-09 -1.33167258927312 3.90791960236807e-09 mirMAP -0.414355153951717 2.6795641701057e-12 NA NA NA hsa-miR-141-5p ITGA9 2.38411349683216 4.56310582620282e-58 -1.80608575406488 8.11052269984858e-33 MirTarget -0.292946163417545 1.02122866661168e-18 NA NA NA hsa-miR-181a-5p ITGA9 0.635863607630183 6.72486918157744e-11 -1.80608575406488 8.11052269984858e-33 mirMAP -0.1566341947306 0.00445464433805693 NA NA NA hsa-miR-181b-5p ITGA9 1.22749257795094 1.21998198830919e-27 -1.80608575406488 8.11052269984858e-33 mirMAP -0.193912679917268 2.88669893981441e-05 NA NA NA hsa-miR-200b-3p ITGA9 2.11744072703209 1.66710572104191e-43 -1.80608575406488 8.11052269984858e-33 mirMAP -0.296491785199914 1.27602275062816e-19 24518294;26334393 β1 integrin inhibition elicits a prometastatic switch through the TGFβ miR 200 ZEB network in E cadherin positive triple negative breast cancer;We revealed that miR-200b suppresses the integrin β1-AKT pathway via targeting Kindlin-2 to mitigate ESCC cell invasiveness ; breast cancer;esophageal cancer hsa-miR-330-3p ITGA9 0.432728362037032 0.00041335506960812 -1.80608575406488 8.11052269984858e-33 mirMAP -0.123337776527911 0.00576598002141124 NA NA NA hsa-miR-33a-5p ITGA9 1.35185127289399 9.80509199125785e-12 -1.80608575406488 8.11052269984858e-33 mirMAP -0.100554823004913 0.000236674119256236 NA NA NA hsa-miR-340-5p ITGA9 1.0857159627596 4.94471062546756e-24 -1.80608575406488 8.11052269984858e-33 MirTarget;mirMAP -0.300344892660173 6.62740693635683e-10 NA NA NA hsa-miR-429 ITGA9 2.84108892259147 3.88520896426515e-64 -1.80608575406488 8.11052269984858e-33 miRanda -0.312074908424021 3.8893701166445e-27 NA NA NA hsa-miR-484 ITGA9 0.349444060004737 0.00154491249625173 -1.80608575406488 8.11052269984858e-33 miRNAWalker2_validate -0.22712257362986 3.26919371898204e-06 NA NA NA hsa-miR-590-3p ITGA9 1.27438512890514 1.03164723481469e-23 -1.80608575406488 8.11052269984858e-33 miRanda -0.215110025646779 6.02900640227352e-07 NA NA NA hsa-miR-590-5p ITGA9 0.746515607868274 8.51300479640125e-09 -1.80608575406488 8.11052269984858e-33 miRanda -0.109684483441211 0.00808092260383434 NA NA NA hsa-miR-664a-3p ITGA9 -0.237699857923843 0.0197071390183375 -1.80608575406488 8.11052269984858e-33 MirTarget -0.164400565077756 0.00198559100484125 NA NA NA hsa-miR-141-3p ITGAV 2.99777540204168 1.26370498493024e-78 -0.0515028444394998 0.572303431365019 MirTarget -0.108251623223024 6.82325620061394e-10 NA NA NA hsa-miR-146b-5p ITGAV 0.631041661167954 6.20366593452965e-08 -0.0515028444394998 0.572303431365019 miRNATAP -0.130383265663822 8.70537168422505e-07 NA NA NA hsa-miR-16-2-3p ITGAV 0.286355254959005 0.0256946046884474 -0.0515028444394998 0.572303431365019 mirMAP -0.114151130652451 2.89132415975006e-06 NA NA NA hsa-miR-200a-3p ITGAV 2.55218480823434 2.96135446816238e-51 -0.0515028444394998 0.572303431365019 MirTarget -0.10013577528264 6.24202667621143e-09 NA NA NA hsa-miR-25-3p ITGAV -0.00546790775985073 0.944061771389468 -0.0515028444394998 0.572303431365019 MirTarget;miRNATAP -0.370376073430225 7.01243332686135e-21 NA NA NA hsa-miR-32-5p ITGAV 1.38676603945192 2.29428299685976e-34 -0.0515028444394998 0.572303431365019 miRNAWalker2_validate;MirTarget;miRNATAP -0.156084602649262 2.65782272596475e-09 NA NA NA hsa-miR-340-5p ITGAV 1.0857159627596 4.94471062546756e-24 -0.0515028444394998 0.572303431365019 MirTarget -0.200546887202324 7.77472998675104e-13 NA NA NA hsa-miR-361-5p ITGAV -0.0996867547900457 0.095428250126326 -0.0515028444394998 0.572303431365019 MirTarget;miRanda -0.446958137818793 9.60789577977041e-18 NA NA NA hsa-miR-374a-5p ITGAV -0.239709888246804 0.00309819667153627 -0.0515028444394998 0.572303431365019 mirMAP -0.196885558896755 3.2752597700007e-07 NA NA NA hsa-miR-374b-5p ITGAV -0.466781429946153 1.17238311619116e-08 -0.0515028444394998 0.572303431365019 mirMAP -0.170952398660091 6.70571303305635e-06 NA NA NA hsa-miR-429 ITGAV 2.84108892259147 3.88520896426515e-64 -0.0515028444394998 0.572303431365019 miRanda -0.104635159336959 9.38637410830064e-10 NA NA NA hsa-miR-590-3p ITGAV 1.27438512890514 1.03164723481469e-23 -0.0515028444394998 0.572303431365019 MirTarget;PITA;miRanda -0.119529282212692 1.60610663897823e-06 NA NA NA hsa-miR-92a-3p ITGAV -0.342420481092807 0.000732275969501111 -0.0515028444394998 0.572303431365019 MirTarget;miRNATAP -0.195743851357691 1.45632172375267e-10 NA NA NA hsa-miR-29b-3p ITGB1 0.438847962603729 6.46722433236121e-05 -0.678317106156189 5.66194666134702e-21 MirTarget;miRNATAP -0.152257153554482 2.41999128688943e-11 NA NA NA hsa-miR-29c-3p ITGB1 -0.0652732378039769 0.563774115756922 -0.678317106156189 5.66194666134702e-21 MirTarget;miRNATAP -0.247090129124282 4.86511208246984e-30 26766915 MiR 29c inhibits cell growth invasion and migration of pancreatic cancer by targeting ITGB1; Further analysis revealed that ITGB1 is one of the functional target genes of miR-29c and knockdown of ITGB1 inhibited the proliferation migration and invasion of PC cells which was similar to the effects of overexpression of miR-29c pancreatic cancer hsa-miR-429 ITGB1 2.84108892259147 3.88520896426515e-64 -0.678317106156189 5.66194666134702e-21 miRanda -0.133174997661172 1.66767130152533e-22 NA NA NA hsa-let-7d-5p ITGB3 0.410958462607207 6.07808182756787e-06 -1.34505322338581 2.68734688128446e-18 MirTarget -0.226787731014433 0.00013304304139482 NA NA NA hsa-miR-146b-5p ITGB3 0.631041661167954 6.20366593452965e-08 -1.34505322338581 2.68734688128446e-18 miRanda -0.153799435346728 0.000833490792102149 NA NA NA hsa-miR-185-5p ITGB3 0.482334746589337 3.68475879959415e-09 -1.34505322338581 2.68734688128446e-18 MirTarget -0.304965495253094 3.39457628789725e-06 NA NA NA hsa-miR-30b-5p ITGB3 -0.46540603860146 2.4709940429591e-05 -1.34505322338581 2.68734688128446e-18 mirMAP -0.204346006917438 2.74563362212737e-05 20498642 Enforced constitutive expression of mir-30 in BT-ICs inhibits their self-renewal capacity by reducing Ubc9 and induces apoptosis through silencing ITGB3 breast cancer hsa-miR-30d-5p ITGB3 -0.247082219011045 0.00406717930063148 -1.34505322338581 2.68734688128446e-18 miRNATAP -0.250653743046726 6.86760348205262e-05 NA NA NA hsa-miR-320b ITGB3 0.412787504747938 0.00113457276903985 -1.34505322338581 2.68734688128446e-18 miRanda -0.153816296240077 0.000316895415818085 NA NA NA hsa-miR-425-5p ITGB3 0.719898949241718 6.01079610190374e-08 -1.34505322338581 2.68734688128446e-18 MirTarget -0.161987450170202 5.78628325273088e-05 NA NA NA hsa-miR-429 ITGB3 2.84108892259147 3.88520896426515e-64 -1.34505322338581 2.68734688128446e-18 miRanda -0.310603398492102 6.39753034365381e-27 NA NA NA hsa-miR-589-5p ITGB3 0.367182541239722 1.67804815542873e-05 -1.34505322338581 2.68734688128446e-18 MirTarget -0.276791248608563 1.19994930769854e-05 NA NA NA hsa-miR-590-3p ITGB3 1.27438512890514 1.03164723481469e-23 -1.34505322338581 2.68734688128446e-18 PITA;miRanda -0.288689456241701 1.55218406303079e-11 NA NA NA hsa-miR-590-5p ITGB3 0.746515607868274 8.51300479640125e-09 -1.34505322338581 2.68734688128446e-18 miRanda -0.246206813772265 1.96120756746322e-09 NA NA NA hsa-miR-98-5p ITGB3 0.656336869298973 9.03113188590466e-15 -1.34505322338581 2.68734688128446e-18 miRNAWalker2_validate;MirTarget -0.411301106439792 4.66935458245862e-11 26445551 miR 98 targets ITGB3 to inhibit proliferation migration and invasion of non small cell lung cancer; Furthermore we identified that miR-98 exerted inhibitory roles by directly binding to 3'-UTR of ITGB3 mRNA thus negatively regulated the expression of ITGB3; Interestingly upon restoring the expression of ITGB3 the effect of miR-98 on cell proliferation was partially reversed; Our findings suggest that miR-98 prevents proliferation migration and invasion of lung cancer cells by directly binding to the 3'-UTR of ITGB3 mRNA and could be a promising treatment option in anticancer therapy lung squamous cell cancer hsa-miR-16-5p ITGB4 0.46835991343556 1.91125356547776e-06 -0.721704740367056 6.04370035561946e-06 miRNAWalker2_validate -0.254895911174257 4.54993259813962e-06 NA NA NA hsa-miR-155-5p ITGB5 1.09766314984151 4.55650930885814e-15 0.194292620009985 0.0495928541847793 miRNAWalker2_validate -0.22825351522107 8.103079189556e-23 NA NA NA hsa-miR-361-5p ITGB5 -0.0996867547900457 0.095428250126326 0.194292620009985 0.0495928541847793 miRanda -0.1559317416226 0.00689088652431586 NA NA NA hsa-miR-378a-5p ITGB5 -2.43173551693523 1.44932837789175e-68 0.194292620009985 0.0495928541847793 MirTarget -0.136596769908688 7.57897389611419e-10 NA NA NA hsa-miR-484 ITGB5 0.349444060004737 0.00154491249625173 0.194292620009985 0.0495928541847793 miRNATAP -0.21252315870238 2.65861108593946e-12 NA NA NA hsa-miR-17-5p ITGB6 0.599531757298529 1.07318660415906e-05 -0.0559941318245576 0.830392713572643 TargetScan -0.348799569129861 8.46187370129024e-08 NA NA NA hsa-miR-197-3p ITGB6 -0.526589453596294 3.61594599437195e-06 -0.0559941318245576 0.830392713572643 MirTarget -0.216400152467493 0.00588762065397408 NA NA NA hsa-miR-181a-5p ITGB8 0.635863607630183 6.72486918157744e-11 -1.12696988747201 4.22273879817388e-08 miRNATAP -0.22913041995449 0.00154711030187845 NA NA NA hsa-miR-181b-5p ITGB8 1.22749257795094 1.21998198830919e-27 -1.12696988747201 4.22273879817388e-08 miRNATAP -0.193110441022224 0.00157578550753994 NA NA NA hsa-miR-182-5p ITGB8 2.36408084103818 2.60011457600281e-72 -1.12696988747201 4.22273879817388e-08 MirTarget -0.211281431029455 1.77955245600773e-05 NA NA NA hsa-miR-183-5p ITGB8 2.95681814393872 1.09965777344285e-92 -1.12696988747201 4.22273879817388e-08 MirTarget -0.18162729391263 3.05273919450045e-05 NA NA NA hsa-miR-338-3p ITGB8 0.0799903526884966 0.575137668326679 -1.12696988747201 4.22273879817388e-08 miRanda -0.131503165247955 0.00838243255881983 NA NA NA hsa-miR-454-3p ITGB8 1.28177672191095 6.84024979484587e-21 -1.12696988747201 4.22273879817388e-08 MirTarget -0.154091676524235 0.0025487540357266 NA NA NA hsa-miR-106b-5p JAK1 1.22477789913175 2.31787213143326e-32 -0.707887486984647 1.74800811102416e-25 miRNAWalker2_validate;MirTarget -0.237214698790733 4.89804513217782e-28 NA NA NA hsa-miR-128-3p JAK1 0.90296715955238 9.925204823863e-23 -0.707887486984647 1.74800811102416e-25 miRNAWalker2_validate -0.207402426169231 1.10214403924094e-16 NA NA NA hsa-miR-15b-5p JAK1 0.788659356634696 1.13678211092534e-11 -0.707887486984647 1.74800811102416e-25 miRNAWalker2_validate -0.136277035192922 1.43927343310931e-11 NA NA NA hsa-miR-17-5p JAK1 0.599531757298529 1.07318660415906e-05 -0.707887486984647 1.74800811102416e-25 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.130465097982384 4.14656265736306e-14 NA NA NA hsa-miR-20a-5p JAK1 0.44979911120222 0.000602386964327228 -0.707887486984647 1.74800811102416e-25 MirTarget -0.197828753019475 3.07441583635672e-29 NA NA NA hsa-miR-30d-5p JAK1 -0.247082219011045 0.00406717930063148 -0.707887486984647 1.74800811102416e-25 MirTarget -0.107054413197506 0.000126918699137881 NA NA NA hsa-miR-340-5p JAK1 1.0857159627596 4.94471062546756e-24 -0.707887486984647 1.74800811102416e-25 MirTarget -0.203953564859323 7.04769381259905e-22 NA NA NA hsa-miR-455-5p JAK1 0.284641224662773 0.0357572339594277 -0.707887486984647 1.74800811102416e-25 miRanda -0.111576100008034 1.90906613010571e-10 NA NA NA hsa-miR-93-5p JAK1 1.01788600209314 1.24312921618126e-21 -0.707887486984647 1.74800811102416e-25 MirTarget;miRNATAP -0.254149430182059 2.35024916289304e-33 NA NA NA hsa-miR-181b-5p JAK2 1.22749257795094 1.21998198830919e-27 -0.676025901327081 1.66897086621935e-13 mirMAP -0.244633185822312 7.80530437489859e-20 NA NA NA hsa-miR-30b-3p JAK3 -0.536462824497982 2.41814659272548e-05 0.393594308074697 0.00524109684441821 MirTarget -0.214091337771 1.67341044412352e-08 NA NA NA hsa-miR-15a-5p KDR 0.812445324157528 4.50263417099822e-20 -1.26178488366313 1.81783097872036e-35 miRNATAP -0.352492195366687 4.38886869055548e-19 NA NA NA hsa-miR-15b-5p KDR 0.788659356634696 1.13678211092534e-11 -1.26178488366313 1.81783097872036e-35 miRNATAP -0.158920943213795 2.46798947112166e-07 NA NA NA hsa-miR-19b-1-5p KDR 0.647704962003062 3.07687667945597e-07 -1.26178488366313 1.81783097872036e-35 miRNAWalker2_validate -0.228393316601211 4.50410521305737e-16 NA NA NA hsa-miR-200b-3p KDR 2.11744072703209 1.66710572104191e-43 -1.26178488366313 1.81783097872036e-35 miRNAWalker2_validate;miRTarBase;TargetScan -0.262999260406618 3.87053169124588e-34 NA NA NA hsa-miR-200c-3p KDR 2.07038651806183 6.84613386286599e-51 -1.26178488366313 1.81783097872036e-35 miRNATAP -0.289279648433676 3.71227459751374e-34 24205206 MiR 200c increases the radiosensitivity of non small cell lung cancer cell line A549 by targeting VEGF VEGFR2 pathway; MiR-200c at the nexus of epithelial-mesenchymal transition EMT is predicted to target VEGFR2; The purpose of this study is to test the hypothesis that regulation of VEGFR2 pathway by miR-200c could modulate the radiosensitivity of cancer cells; Bioinformatic analysis luciferase reporter assays and biochemical assays were carried out to validate VEGFR2 as a direct target of miR-200c; We identified VEGFR2 as a novel target of miR-200c lung squamous cell cancer hsa-miR-3065-3p KDR 1.62787885002468 6.81477830005026e-19 -1.26178488366313 1.81783097872036e-35 miRNATAP -0.151833949842702 1.66297752718813e-15 NA NA NA hsa-miR-429 KDR 2.84108892259147 3.88520896426515e-64 -1.26178488366313 1.81783097872036e-35 PITA;miRanda;miRNATAP -0.244793933063007 3.13570384816226e-37 NA NA NA hsa-miR-455-5p KDR 0.284641224662773 0.0357572339594277 -1.26178488366313 1.81783097872036e-35 PITA;miRanda;miRNATAP -0.184306159980816 3.7489040476857e-12 NA NA NA hsa-miR-590-3p KDR 1.27438512890514 1.03164723481469e-23 -1.26178488366313 1.81783097872036e-35 miRanda -0.164817675844501 1.21955605160493e-08 NA NA NA hsa-miR-590-5p KDR 0.746515607868274 8.51300479640125e-09 -1.26178488366313 1.81783097872036e-35 miRanda -0.121363305137282 1.22819221367569e-05 NA NA NA hsa-let-7a-3p KIT 0.466622567891891 3.70886257943079e-07 -3.55980779277124 2.17700605158782e-58 mirMAP -0.305708258630377 0.000563948340312811 NA NA NA hsa-miR-128-3p KIT 0.90296715955238 9.925204823863e-23 -3.55980779277124 2.17700605158782e-58 MirTarget -0.716184412196196 4.01386964924962e-17 NA NA NA hsa-miR-130b-3p KIT 1.38122163823846 1.00932179737836e-26 -3.55980779277124 2.17700605158782e-58 miRNATAP -0.463647857585747 2.22404067928257e-14 NA NA NA hsa-miR-193a-3p KIT 0.310617999804917 0.00477640270974957 -3.55980779277124 2.17700605158782e-58 MirTarget;PITA;miRanda;miRNATAP -0.423244385923827 9.1952492703228e-09 NA NA NA hsa-miR-193b-3p KIT -0.0550374855074773 0.65382485898551 -3.55980779277124 2.17700605158782e-58 MirTarget;miRNATAP -0.456397182897406 4.23711519022461e-12 NA NA NA hsa-miR-301a-3p KIT 1.32170168375632 2.12280368957514e-15 -3.55980779277124 2.17700605158782e-58 miRNATAP -0.378579845243396 1.98724994161281e-15 NA NA NA hsa-miR-338-3p KIT 0.0799903526884966 0.575137668326679 -3.55980779277124 2.17700605158782e-58 miRanda -0.311406099865202 4.36514658431642e-08 NA NA NA hsa-miR-342-3p KIT 1.21944481975657 6.81914373001202e-19 -3.55980779277124 2.17700605158782e-58 miRanda -0.435927652877587 2.76373234511638e-14 NA NA NA hsa-miR-34a-5p KIT 0.129201805242403 0.133261654068224 -3.55980779277124 2.17700605158782e-58 MirTarget -0.444920568628705 2.86104030251141e-06 NA NA NA hsa-miR-429 KIT 2.84108892259147 3.88520896426515e-64 -3.55980779277124 2.17700605158782e-58 miRNATAP -0.336829033851034 3.36923704405637e-14 NA NA NA hsa-miR-454-3p KIT 1.28177672191095 6.84024979484587e-21 -3.55980779277124 2.17700605158782e-58 miRNATAP -0.531899770931243 1.67917065615985e-20 NA NA NA hsa-miR-590-3p KIT 1.27438512890514 1.03164723481469e-23 -3.55980779277124 2.17700605158782e-58 miRanda;mirMAP -0.400477071862 6.4525241042505e-10 NA NA NA hsa-miR-671-5p KIT 1.55248047544015 7.7377784125886e-33 -3.55980779277124 2.17700605158782e-58 MirTarget -0.668037376396378 2.34590667378775e-29 NA NA NA hsa-miR-142-5p KITLG 1.16509303143593 1.96641162209073e-13 -0.144677358802896 0.348643719752218 MirTarget;PITA -0.135701636636652 3.48772477572693e-05 NA NA NA hsa-miR-17-3p KITLG 0.721439431208839 7.95900548819742e-10 -0.144677358802896 0.348643719752218 MirTarget -0.552597905855487 2.30753379596766e-38 NA NA NA hsa-miR-181a-5p KITLG 0.635863607630183 6.72486918157744e-11 -0.144677358802896 0.348643719752218 MirTarget -0.145945916834505 0.00678539392102327 NA NA NA hsa-miR-181b-5p KITLG 1.22749257795094 1.21998198830919e-27 -0.144677358802896 0.348643719752218 MirTarget -0.152369506837405 0.000802960531821635 NA NA NA hsa-miR-181c-5p KITLG 0.574847839298605 4.51287502066478e-07 -0.144677358802896 0.348643719752218 MirTarget -0.279559899195876 1.07150063863304e-09 NA NA NA hsa-miR-181d-5p KITLG 0.782618466877349 1.2821116358212e-09 -0.144677358802896 0.348643719752218 MirTarget -0.239492059331143 2.9152716697973e-09 NA NA NA hsa-miR-185-5p KITLG 0.482334746589337 3.68475879959415e-09 -0.144677358802896 0.348643719752218 miRNAWalker2_validate -0.42377124964546 3.25274076407301e-11 NA NA NA hsa-miR-186-5p KITLG -0.426987301460247 1.04129380549315e-05 -0.144677358802896 0.348643719752218 mirMAP -0.428668898343776 1.44268570297994e-15 NA NA NA hsa-miR-19a-3p KITLG 0.817507952171848 3.76285090469625e-08 -0.144677358802896 0.348643719752218 mirMAP -0.366984530985126 8.2317505317704e-27 NA NA NA hsa-miR-19b-3p KITLG 0.139124391010031 0.255658553872934 -0.144677358802896 0.348643719752218 mirMAP -0.416970084520976 4.95393846829457e-23 NA NA NA hsa-miR-22-5p KITLG 0.238328801322329 0.0377496760295305 -0.144677358802896 0.348643719752218 MirTarget -0.181446661957323 8.28817908890659e-05 NA NA NA hsa-miR-222-3p KITLG -0.429279381480379 0.00103945569781115 -0.144677358802896 0.348643719752218 miRNAWalker2_validate -0.246785551483141 6.9109803255638e-10 NA NA NA hsa-miR-23a-3p KITLG -0.217176729033818 0.00962615056202409 -0.144677358802896 0.348643719752218 mirMAP -0.246686544703499 9.72747872547485e-05 NA NA NA hsa-miR-30c-5p KITLG -0.393222674580066 8.06961465717306e-05 -0.144677358802896 0.348643719752218 mirMAP -0.154707158437856 0.00353873917775031 NA NA NA hsa-miR-30e-5p KITLG 0.293491764699475 0.000372291967271834 -0.144677358802896 0.348643719752218 mirMAP -0.479980922617283 4.095991313302e-14 NA NA NA hsa-miR-320b KITLG 0.412787504747938 0.00113457276903985 -0.144677358802896 0.348643719752218 MirTarget;PITA;miRanda -0.251791916761997 1.35049162381435e-09 NA NA NA hsa-miR-345-5p KITLG 0.639728356429512 0.00013070715719672 -0.144677358802896 0.348643719752218 MirTarget -0.110868562663438 0.000425939265168191 NA NA NA hsa-miR-34a-5p KITLG 0.129201805242403 0.133261654068224 -0.144677358802896 0.348643719752218 MirTarget -0.193013612056888 0.00180458841369172 NA NA NA hsa-miR-374a-5p KITLG -0.239709888246804 0.00309819667153627 -0.144677358802896 0.348643719752218 mirMAP -0.198926480140352 0.00244094214640679 NA NA NA hsa-miR-374b-5p KITLG -0.466781429946153 1.17238311619116e-08 -0.144677358802896 0.348643719752218 mirMAP -0.239181492580703 0.000205968556730923 NA NA NA hsa-miR-589-3p KITLG 0.0263457630961427 0.866577983997705 -0.144677358802896 0.348643719752218 mirMAP -0.134074718980368 0.000100452076952808 NA NA NA hsa-miR-590-3p KITLG 1.27438512890514 1.03164723481469e-23 -0.144677358802896 0.348643719752218 MirTarget;PITA;miRanda -0.285492852241295 9.72509971848425e-12 NA NA NA hsa-miR-9-5p KITLG 0.734772328078424 0.00261982843636383 -0.144677358802896 0.348643719752218 MirTarget -0.220147209748711 7.89179813840006e-26 NA NA NA hsa-miR-140-3p KRAS -1.25844395244141 5.29222651039892e-33 0.46818988509622 3.00748217900386e-10 MirTarget -0.245149449217664 6.89860831179258e-27 NA NA NA hsa-miR-143-3p KRAS -1.23248181631499 4.74160976678504e-28 0.46818988509622 3.00748217900386e-10 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.124135749972866 1.80319700574441e-08 22804917;27725862;19137007;26581910;23173124;26824186;21197560 In KRAS mutated tumours increased miR-200b and decreased miR-143 expression were associated with a good PFS;The expression of miR-143 is often down-regulated and it might play an important role by targeting KRAS in colorectal cancer CRC; Dual-luciferase reporter assays were performed to validate whether KRAS was regulated by miR-143; Dual-luciferase assays indicated that KRAS was a direct target of miR-143 as subsequently demonstrated by qPCR and western blot analysis showing that infection of SW480 cells with Ad-ZD55-miR-143 resulted in the down-regulation of KRAS at both mRNA and protein levels;Role of miR 143 targeting KRAS in colorectal tumorigenesis; In particular among a panel of presumed targets generated by in silico analysis that may interact with these aberrantly expressed miRNAs KRAS oncogene has been further experimentally validated as the target of miR-143; First an inverse correlation between KRAS protein and miR-143 in vivo was found; Second KRAS expression in Lovo cells was significantly abolished by treatment with miR-143 mimic whereas miR-143 inhibitor increased KRAS protein level; Third luciferase reporter assay confirmed that miR-143 directly recognize the 3'-untranslated region of KRAS transcripts; Finally inhibition of KRAS expression by miR-143 inhibits constitutive phosphorylation of ERK1/2; Taken together the present study provides the first evidences that miR-143 is significant in suppressing colorectal cancer cell growth through inhibition of KRAS translation;MicroRNA 143 replenishment re sensitizes colorectal cancer cells harboring mutant but not wild type KRAS to paclitaxel treatment; Our results showed that miR-143 but not let-7b increased sensitization of KRAS mutant tumor cells to paclitaxel; Furthermore transfection of miR-143 but not let-7b mimic negatively regulated the expression of mutant but not wild-type KRAS; Combination of miR-143 mimic and paclitaxel induced the onset of apoptosis and reverted in vitro metastatic properties migration and invasion in KRAS mutant tumor cells; MiR-143 thus can be used as a chemosensitizer for the treatment of KRAS mutant tumors and warrants further investigations in in vitro and pre-clinical in vivo models;In turn the level of miR-143 in CRC cells decreasing the amount of MACC1 metastasis-associated in colon cancer-1 and oncogenic KRAS protein may be utilized as a prognostic marker;Stable miR-143 or miR-145 overexpression increased cell sensitivity to cetuximab resulting in a significant increase of cetuximab-mediated ADCC independently of KRAS status;miR 143 decreases prostate cancer cells proliferation and migration and enhances their sensitivity to docetaxel through suppression of KRAS; The expression level of miR-143 and its target gene KRAS were measured by realtime PCR and western blotting respectively; Our results revealed an inverse correlation of expression between miR-143 and KRAS protein in prostate cancer samples Pearson's correlation scatter plots: R = -0.707 P < 0.05; These findings suggest that miR-143 plays an important role in prostate cancer proliferation migration and chemosensitivity by suppressing KRAS and subsequent inactivation of MAPK pathway which provides a potential development of a new approach for the treatment of prostate cancer ;;tumorigenesis;;metastasis;; colorectal cancer;colorectal cancer;colorectal cancer;colorectal cancer;colorectal cancer;colon cancer;prostate cancer hsa-miR-195-3p KRAS -1.49582726452416 6.53261924105147e-40 0.46818988509622 3.00748217900386e-10 mirMAP -0.144084535908296 1.9114583860958e-11 NA NA NA hsa-miR-30d-5p KRAS -0.247082219011045 0.00406717930063148 0.46818988509622 3.00748217900386e-10 mirMAP;miRNATAP -0.13177346252977 1.09256098608436e-05 NA NA NA hsa-miR-542-3p KRAS 0.568822103820889 8.5699075257651e-07 0.46818988509622 3.00748217900386e-10 miRanda -0.123003016716369 2.33095765684339e-08 NA NA NA hsa-miR-342-3p LAMA1 1.21944481975657 6.81914373001202e-19 -1.41719202732824 1.99645859303e-12 miRanda -0.373365420839019 3.17298377107165e-14 NA NA NA hsa-miR-146b-5p LAMA2 0.631041661167954 6.20366593452965e-08 -2.16756236589159 1.88679943487983e-31 miRanda -0.179973081651138 0.00143455201383938 NA NA NA hsa-miR-375 LAMA2 2.09893673049766 1.68351780396203e-16 -2.16756236589159 1.88679943487983e-31 miRanda -0.141366224796711 2.30583404419529e-08 NA NA NA hsa-miR-590-3p LAMA2 1.27438512890514 1.03164723481469e-23 -2.16756236589159 1.88679943487983e-31 miRanda -0.585831805457993 2.67005129674139e-30 NA NA NA hsa-miR-17-5p LAMA3 0.599531757298529 1.07318660415906e-05 -2.53251208906831 2.18425216006227e-32 TargetScan -0.410037720264012 9.13613581227756e-14 NA NA NA hsa-miR-181a-5p LAMA3 0.635863607630183 6.72486918157744e-11 -2.53251208906831 2.18425216006227e-32 miRNAWalker2_validate -0.515885630770767 1.96293779720287e-11 NA NA NA hsa-miR-590-3p LAMA3 1.27438512890514 1.03164723481469e-23 -2.53251208906831 2.18425216006227e-32 miRanda -0.514039700190172 1.03861305236823e-17 NA NA NA hsa-miR-21-5p LAMA4 2.32007501846389 3.08394781855554e-137 -1.35277171982831 9.76622188586797e-36 MirTarget -0.249548681112465 8.17325028836981e-13 NA NA NA hsa-miR-429 LAMA4 2.84108892259147 3.88520896426515e-64 -1.35277171982831 9.76622188586797e-36 miRanda -0.307419941987586 8.34841968593741e-53 NA NA NA hsa-miR-590-3p LAMA4 1.27438512890514 1.03164723481469e-23 -1.35277171982831 9.76622188586797e-36 miRanda -0.24483340239778 1.29870009663173e-15 NA NA NA hsa-miR-590-5p LAMA4 0.746515607868274 8.51300479640125e-09 -1.35277171982831 9.76622188586797e-36 MirTarget;miRanda -0.176702647528711 2.11847965347872e-09 NA NA NA hsa-miR-93-3p LAMA4 0.627759242044891 3.62527588304617e-06 -1.35277171982831 9.76622188586797e-36 miRNAWalker2_validate -0.109203388533782 0.000125334100204899 NA NA NA hsa-let-7a-3p LAMB1 0.466622567891891 3.70886257943079e-07 -0.359449827932765 0.000369001424098615 MirTarget -0.152234530153535 5.2428651237853e-05 NA NA NA hsa-miR-16-5p LAMB1 0.46835991343556 1.91125356547776e-06 -0.359449827932765 0.000369001424098615 miRNAWalker2_validate -0.14935846586241 2.10153923705453e-05 NA NA NA hsa-miR-339-5p LAMB1 -0.211548290465418 0.0685885445822389 -0.359449827932765 0.000369001424098615 miRanda -0.131454123943774 1.04413742156432e-05 NA NA NA hsa-miR-29b-1-5p LAMB2 0.0164831140865254 0.909727358526678 -0.627408163144899 1.14916224243242e-10 MirTarget -0.119137375812176 2.91965085101432e-07 NA NA NA hsa-miR-484 LAMB2 0.349444060004737 0.00154491249625173 -0.627408163144899 1.14916224243242e-10 miRNAWalker2_validate -0.233692536644385 8.56394221947151e-15 NA NA NA hsa-miR-16-5p LAMB3 0.46835991343556 1.91125356547776e-06 -2.04349199041731 6.00391362293159e-24 miRNAWalker2_validate -0.569347376189091 1.52818791510295e-15 NA NA NA hsa-miR-324-5p LAMB3 0.211552647440779 0.139408130311654 -2.04349199041731 6.00391362293159e-24 miRanda -0.198898358733369 6.62432446427303e-05 NA NA NA hsa-miR-484 LAMB3 0.349444060004737 0.00154491249625173 -2.04349199041731 6.00391362293159e-24 MirTarget;miRNATAP -0.456535599050427 9.75927556664892e-13 NA NA NA hsa-miR-107 LAMC1 0.678998026350662 2.56224148930538e-16 -1.22673201326814 1.35102621663939e-35 miRanda -0.257698330142802 6.41811392129414e-10 NA NA NA hsa-miR-15a-5p LAMC1 0.812445324157528 4.50263417099822e-20 -1.22673201326814 1.35102621663939e-35 MirTarget -0.326629832851419 1.81370836508136e-17 NA NA NA hsa-miR-15b-5p LAMC1 0.788659356634696 1.13678211092534e-11 -1.22673201326814 1.35102621663939e-35 MirTarget -0.106501584548076 0.000387350984312645 NA NA NA hsa-miR-182-5p LAMC1 2.36408084103818 2.60011457600281e-72 -1.22673201326814 1.35102621663939e-35 miRNATAP -0.279210293932062 2.59063689304366e-32 NA NA NA hsa-miR-193a-3p LAMC1 0.310617999804917 0.00477640270974957 -1.22673201326814 1.35102621663939e-35 MirTarget;PITA;miRanda -0.114858064497944 0.000338849778603983 NA NA NA hsa-miR-200b-3p LAMC1 2.11744072703209 1.66710572104191e-43 -1.22673201326814 1.35102621663939e-35 MirTarget;TargetScan -0.289967230006364 9.75181041203852e-45 NA NA NA hsa-miR-200c-3p LAMC1 2.07038651806183 6.84613386286599e-51 -1.22673201326814 1.35102621663939e-35 MirTarget;miRNATAP -0.329360689529761 5.25538223517216e-48 NA NA NA hsa-miR-29b-3p LAMC1 0.438847962603729 6.46722433236121e-05 -1.22673201326814 1.35102621663939e-35 MirTarget;miRNATAP -0.26005932104654 1.6052896437976e-16 NA NA NA hsa-miR-29c-3p LAMC1 -0.0652732378039769 0.563774115756922 -1.22673201326814 1.35102621663939e-35 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.293087073092568 8.3737259345595e-22 NA NA NA hsa-miR-338-3p LAMC1 0.0799903526884966 0.575137668326679 -1.22673201326814 1.35102621663939e-35 MirTarget;miRanda -0.109223045761013 9.54907872319034e-06 NA NA NA hsa-miR-429 LAMC1 2.84108892259147 3.88520896426515e-64 -1.22673201326814 1.35102621663939e-35 MirTarget;PITA;miRanda;miRNATAP -0.250412580267939 1.24821064577774e-41 NA NA NA hsa-miR-590-3p LAMC1 1.27438512890514 1.03164723481469e-23 -1.22673201326814 1.35102621663939e-35 PITA;miRanda;mirMAP -0.173527924429224 5.97096955178295e-10 NA NA NA hsa-miR-96-5p LAMC1 3.24596558166606 1.92296159041707e-98 -1.22673201326814 1.35102621663939e-35 MirTarget;TargetScan;miRNATAP -0.258001814246817 1.73137300974635e-40 NA NA NA hsa-miR-98-5p LAMC1 0.656336869298973 9.03113188590466e-15 -1.22673201326814 1.35102621663939e-35 miRNAWalker2_validate -0.166984209258336 4.95571574796494e-05 NA NA NA hsa-miR-148b-3p LAMC2 1.0552576751879 1.6679285705231e-29 -1.62034326586173 2.05014148904585e-12 miRNAWalker2_validate -0.468080437420498 1.33999489210089e-08 NA NA NA hsa-miR-193a-3p LAMC2 0.310617999804917 0.00477640270974957 -1.62034326586173 2.05014148904585e-12 MirTarget;PITA;miRanda;miRNATAP -0.373136854988213 2.63946875891495e-07 NA NA NA hsa-miR-193b-3p LAMC2 -0.0550374855074773 0.65382485898551 -1.62034326586173 2.05014148904585e-12 MirTarget;miRNATAP -0.210485711342769 0.00129019619433589 NA NA NA hsa-miR-3065-3p LAMC2 1.62787885002468 6.81477830005026e-19 -1.62034326586173 2.05014148904585e-12 miRNATAP -0.126910675281551 0.00295289352008255 NA NA NA hsa-miR-338-3p LAMC2 0.0799903526884966 0.575137668326679 -1.62034326586173 2.05014148904585e-12 miRanda -0.191701598436574 0.000640679213604702 NA NA NA hsa-miR-342-3p LAMC2 1.21944481975657 6.81914373001202e-19 -1.62034326586173 2.05014148904585e-12 miRanda -0.273518493093315 1.49370659196398e-06 NA NA NA hsa-miR-375 LAMC2 2.09893673049766 1.68351780396203e-16 -1.62034326586173 2.05014148904585e-12 miRanda -0.225853976513003 1.07683110051097e-13 NA NA NA hsa-miR-130b-3p LAMC3 1.38122163823846 1.00932179737836e-26 -1.32002986076632 4.64847430467652e-10 mirMAP -0.192590376790565 0.000506731874312583 NA NA NA hsa-miR-185-3p LAMC3 0.86591374114446 7.8790769678542e-10 -1.32002986076632 4.64847430467652e-10 mirMAP -0.221573832064258 2.28493264466774e-05 NA NA NA hsa-miR-192-5p LAMC3 1.33366224451777 9.5116159697107e-29 -1.32002986076632 4.64847430467652e-10 mirMAP -0.37466566954641 1.70512132569454e-10 NA NA NA hsa-miR-301a-3p LAMC3 1.32170168375632 2.12280368957514e-15 -1.32002986076632 4.64847430467652e-10 mirMAP -0.278814165392613 1.05673769172482e-10 NA NA NA hsa-miR-324-3p LAMC3 0.404877731996915 0.000340036423920834 -1.32002986076632 4.64847430467652e-10 mirMAP -0.274441696030032 2.25756338680375e-05 NA NA NA hsa-miR-330-5p LAMC3 0.626063225953928 3.42215028606779e-09 -1.32002986076632 4.64847430467652e-10 MirTarget;miRanda -0.292950727962664 1.9970694272922e-05 NA NA NA hsa-miR-425-5p LAMC3 0.719898949241718 6.01079610190374e-08 -1.32002986076632 4.64847430467652e-10 mirMAP -0.319956967959409 4.25190999181462e-09 NA NA NA hsa-miR-16-2-3p LPAR1 0.286355254959005 0.0256946046884474 -1.20736956265381 1.93522163393847e-21 MirTarget;mirMAP -0.19697282693615 1.5034723198793e-08 NA NA NA hsa-miR-200b-3p LPAR1 2.11744072703209 1.66710572104191e-43 -1.20736956265381 1.93522163393847e-21 MirTarget;TargetScan -0.30751425904766 7.7026883287757e-31 NA NA NA hsa-miR-200c-3p LPAR1 2.07038651806183 6.84613386286599e-51 -1.20736956265381 1.93522163393847e-21 miRNAWalker2_validate;MirTarget;miRNATAP -0.351153536982569 2.48589736261259e-33 NA NA NA hsa-miR-20a-3p LPAR1 -0.218902047543766 0.179868363625547 -1.20736956265381 1.93522163393847e-21 MirTarget -0.129594126969158 2.12761658223871e-06 NA NA NA hsa-miR-324-5p LPAR1 0.211552647440779 0.139408130311654 -1.20736956265381 1.93522163393847e-21 miRanda -0.176023078692938 1.36136378704964e-08 NA NA NA hsa-miR-342-3p LPAR1 1.21944481975657 6.81914373001202e-19 -1.20736956265381 1.93522163393847e-21 miRanda -0.103938094662758 0.00108015667344116 NA NA NA hsa-miR-361-5p LPAR1 -0.0996867547900457 0.095428250126326 -1.20736956265381 1.93522163393847e-21 miRanda -0.207471661221339 0.00632244085518377 NA NA NA hsa-miR-429 LPAR1 2.84108892259147 3.88520896426515e-64 -1.20736956265381 1.93522163393847e-21 MirTarget;miRanda;miRNATAP -0.284902325794861 2.8630305499812e-33 NA NA NA hsa-miR-576-5p LPAR1 0.0649387707567879 0.588791966096287 -1.20736956265381 1.93522163393847e-21 mirMAP -0.175656580277321 2.21953993247044e-06 NA NA NA hsa-miR-671-5p LPAR1 1.55248047544015 7.7377784125886e-33 -1.20736956265381 1.93522163393847e-21 MirTarget -0.363233883578158 5.80945759008805e-29 NA NA NA hsa-miR-7-1-3p LPAR1 0.686579567507582 1.20731554448806e-07 -1.20736956265381 1.93522163393847e-21 MirTarget -0.132315706715143 0.000106417358728036 NA NA NA hsa-miR-143-3p LPAR2 -1.23248181631499 4.74160976678504e-28 1.45482613879136 2.4402475071835e-52 miRNATAP -0.229390680773225 1.21623659623871e-14 NA NA NA hsa-miR-654-3p LPAR2 -1.20494927978695 4.07682448923698e-16 1.45482613879136 2.4402475071835e-52 MirTarget -0.201997770510525 6.26208275949693e-19 NA NA NA hsa-miR-192-5p LPAR4 1.33366224451777 9.5116159697107e-29 -0.744328364270219 1.68304940061366e-07 MirTarget;miRNATAP -0.151568860607239 0.000127542115024575 NA NA NA hsa-miR-3613-5p LPAR4 1.11067990676844 4.33424951743633e-21 -0.744328364270219 1.68304940061366e-07 miRNATAP -0.136705269862333 0.000794771431451876 NA NA NA hsa-miR-429 LPAR4 2.84108892259147 3.88520896426515e-64 -0.744328364270219 1.68304940061366e-07 miRNATAP -0.242512654278231 6.52512137164503e-20 NA NA NA hsa-miR-590-3p LPAR4 1.27438512890514 1.03164723481469e-23 -0.744328364270219 1.68304940061366e-07 MirTarget;miRanda;mirMAP -0.186896924184631 1.8731513874049e-06 NA NA NA hsa-miR-365a-3p LPAR5 -1.45032725192851 2.37888098061118e-37 0.705348873873176 1.3909840810237e-11 MirTarget -0.16756034195952 3.23777768844922e-08 NA NA NA hsa-miR-24-3p LPAR6 -0.120123317289941 0.21432376743243 -1.15577850867345 1.23453033614464e-42 MirTarget -0.108584259200921 0.00060640600623042 NA NA NA hsa-miR-27a-3p LPAR6 0.388496361379095 1.42373532086331e-05 -1.15577850867345 1.23453033614464e-42 MirTarget;miRNATAP -0.129795423736929 0.000138794680010169 NA NA NA hsa-miR-590-3p LPAR6 1.27438512890514 1.03164723481469e-23 -1.15577850867345 1.23453033614464e-42 miRanda -0.202524663777939 3.30984967978445e-17 NA NA NA hsa-miR-101-3p MAP2K1 -0.126035286271986 0.145997832808138 -0.185433389862149 0.000727711407664528 miRNAWalker2_validate -0.158307400181186 2.0108100169932e-13 NA NA NA hsa-miR-181a-5p MAP2K1 0.635863607630183 6.72486918157744e-11 -0.185433389862149 0.000727711407664528 miRNAWalker2_validate -0.103686555340189 5.16890556712928e-08 NA NA NA hsa-miR-361-5p MAP2K1 -0.0996867547900457 0.095428250126326 -0.185433389862149 0.000727711407664528 miRanda -0.121080950243885 0.000149537457617663 NA NA NA hsa-miR-542-3p MAP2K1 0.568822103820889 8.5699075257651e-07 -0.185433389862149 0.000727711407664528 miRanda -0.113613468340007 1.26995828232255e-12 NA NA NA hsa-let-7b-5p MAP2K2 -0.54215483448257 7.21347617597858e-08 0.61941089334837 7.64917587016439e-15 miRNAWalker2_validate -0.171851964586426 2.69292025446759e-10 NA NA NA hsa-miR-29c-3p MAPK1 -0.0652732378039769 0.563774115756922 -0.282469104608855 4.85723866828359e-06 mirMAP -0.107930467813368 8.09848447187363e-09 NA NA NA hsa-miR-361-5p MAPK1 -0.0996867547900457 0.095428250126326 -0.282469104608855 4.85723866828359e-06 PITA;miRanda;mirMAP -0.263780179525808 1.39108568009728e-13 NA NA NA hsa-miR-3913-5p MAPK1 0.71890413546147 2.61153227160465e-13 -0.282469104608855 4.85723866828359e-06 mirMAP -0.145979254174869 5.54224263447113e-12 NA NA NA hsa-miR-660-5p MAPK1 0.406910761707485 0.000156175371762464 -0.282469104608855 4.85723866828359e-06 mirMAP -0.144747929471906 1.05117786154161e-13 NA NA NA hsa-miR-197-3p MAPK3 -0.526589453596294 3.61594599437195e-06 -0.0806112580526674 0.201094437565328 miRNATAP -0.106370513582673 1.47526386613596e-08 NA NA NA hsa-miR-143-3p MDM2 -1.23248181631499 4.74160976678504e-28 0.134052426741699 0.110706751806459 miRNAWalker2_validate -0.116551192492011 2.45785814867103e-06 NA NA NA hsa-miR-181a-2-3p MDM2 -0.0718643470098321 0.422077954889554 0.134052426741699 0.110706751806459 mirMAP -0.12266836491989 0.000146166192040392 NA NA NA hsa-miR-25-3p MDM2 -0.00546790775985073 0.944061771389468 0.134052426741699 0.110706751806459 miRNAWalker2_validate;miRTarBase -0.121996633743668 0.00105879152857406 NA NA NA hsa-miR-340-5p MDM2 1.0857159627596 4.94471062546756e-24 0.134052426741699 0.110706751806459 mirMAP -0.114356673254404 1.13062337969042e-05 26718483 MicroRNA 340 inhibits prostate cancer cell proliferation and metastasis by targeting the MDM2 p53 pathway; Our findings suggest that miR-340 may function as a novel tumor suppressor in PCa through the MDM2-p53 pathway by directly targeting MDM2 which may be a promising miRNA-targeted therapy for PCa metastasis prostate cancer hsa-miR-369-3p MDM2 0.0880848299432269 0.48519534195924 0.134052426741699 0.110706751806459 mirMAP -0.139668513237869 8.70962517671477e-10 NA NA NA hsa-miR-374a-5p MDM2 -0.239709888246804 0.00309819667153627 0.134052426741699 0.110706751806459 mirMAP -0.181528999029164 3.25892646409229e-07 NA NA NA hsa-miR-374b-5p MDM2 -0.466781429946153 1.17238311619116e-08 0.134052426741699 0.110706751806459 mirMAP -0.113653249109849 0.00120350297983546 NA NA NA hsa-miR-485-3p MDM2 -0.866822427729628 4.5194665263492e-11 0.134052426741699 0.110706751806459 mirMAP -0.105743116773406 1.17706611197258e-06 NA NA NA hsa-miR-500a-5p MDM2 0.209805801129289 0.119177819862398 0.134052426741699 0.110706751806459 mirMAP -0.119455592892776 2.98058844851876e-08 NA NA NA hsa-miR-584-5p MDM2 -1.75458362433615 2.80754771470968e-33 0.134052426741699 0.110706751806459 mirMAP -0.102042062933 5.80205890285492e-08 NA NA NA hsa-miR-30a-5p MET -0.392489994825279 0.00954894667761676 -2.36906628256571 5.79970002931919e-38 miRNAWalker2_validate -0.134503205736039 0.00203853525633078 NA NA NA hsa-miR-34a-5p MET 0.129201805242403 0.133261654068224 -2.36906628256571 5.79970002931919e-38 miRNAWalker2_validate;miRTarBase -0.260533934700572 0.00074892552882336 23243217;26104764;22457788;23593387;19006648;19773441;19029026 Detection of miR 34a promoter methylation in combination with elevated expression of c Met and β catenin predicts distant metastasis of colon cancer; Using a case-control study design of 94 primary colon cancer samples with and without liver metastases we determined CpG methylation frequencies of miR-34a and miR-34b/c promoters expression of miR-34a and its targets c-Met Snail and β-catenin and their prognostic value; Decreased miR-34a expression was associated with upregulation of c-Met Snail and β-catenin protein levels P = 0.031 0.132 and 0.004 which were associated with distant metastases P = 0.001 0.017 and 0.005; In a confounder-adjusted multivariate regression model miR-34a methylation high c-Met and β-catenin levels provided the most significant prognostic information about metastases to the liver P = 0.014 0.031 and 0.058 and matched pairs showed a higher prevalence of these risk factors in the samples with distant spread P = 0.029; Silencing of miR-34a and upregulation of c-Met Snail and β-catenin expression is associated with liver metastases of colon cancer; Detection of miR-34a silencing in resected primary colon cancer may be of prognostic value especially in combination with detection of c-Met and β-catenin expression;The expression of microRNA 34a is inversely correlated with c MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients; We found significant inverse correlations between miR-34a and c-MET R = -0.316 P = 0.028 and CDK6 expression R = -0.4582 P = 0.004;c-Met is a target of miR-34a and regulates the migration and invasion of osteosarcoma cells; Osteosarcoma cells over-expressing miR-34a exhibited a significant decrease in the expression levels of c-Met mRNA and protein simultaneously; The results presented in this study demonstrated that over-expression of miR-34a could inhibit the tumor growth and metastasis of osteosarcoma probably through down regulating c-Met; And there are other putative miR-34a target genes beside c-Met which could potentially be key players in the development of osteosarcoma;Underexpression of miR 34a in hepatocellular carcinoma and its contribution towards enhancement of proliferating inhibitory effects of agents targeting c MET; In addition miR-34a mimic enhanced the effect of cell proliferation inhibition and caspase activity induction of agents targeting c-MET siRNAs and small molecular inhibitor su11274;miR 34a inhibits migration and invasion by down regulation of c Met expression in human hepatocellular carcinoma cells; miR-34a directly targeted c-Met and reduced both mRNA and protein levels of c-Met; thus decreased c-Met-induced phosphorylation of extracellular signal-regulated kinases 1 and 2 ERK1/2; Taken together these results provide evidence to show the suppression role of miR-34a in tumor migration and invasion through modulation of the c-Met signaling pathway;miR-34a levels in human gliomas inversely correlated to c-Met levels measured in the same tumors; Forced expression of c-Met or Notch-1/Notch-2 transcripts lacking the 3'-untranslated region sequences partially reversed the effects of miR-34a on cell cycle arrest and cell death in glioma cells and stem cells respectively; They show that miR-34a suppresses brain tumor growth by targeting c-Met and Notch;MicroRNA 34a inhibits uveal melanoma cell proliferation and migration through downregulation of c Met; In addition expression of c-Met and cell cycle-related proteins was determined by Western blotting and immunofluorescence after the introduction of miR-34a miR-34a is actively expressed in melanocytes but not in uveal melanoma cells based on Northern blot analysis; After identification of two putative miR-34a binding sites within the 3' UTR of the human c-Met mRNA miR-34a was shown to suppress luciferase activity using HEK293 cells with a luciferase reporter construct containing the binding sites; miR-34a was confirmed to downregulate the expression of c-Met protein by Western blotting and immunofluorescence; These results demonstrate that miR-34a acts as a tumor suppressor in uveal melanoma cell proliferation and migration through the downregulation of c-Met metastasis;;metastasis;;;; colon cancer;lung cancer;sarcoma;liver cancer;liver cancer;glioblastoma;melanoma hsa-miR-664a-3p MET -0.237699857923843 0.0197071390183375 -2.36906628256571 5.79970002931919e-38 mirMAP -0.177552910041329 0.00637075964459348 NA NA NA hsa-miR-7-1-3p MET 0.686579567507582 1.20731554448806e-07 -2.36906628256571 5.79970002931919e-38 mirMAP -0.239508473942628 2.05545838272513e-06 NA NA NA hsa-miR-107 MYB 0.678998026350662 2.56224148930538e-16 1.3385004671291 4.8746375657764e-10 miRNAWalker2_validate;miRTarBase;PITA;miRanda;miRNATAP -0.239055082149443 0.00716978817274039 NA NA NA hsa-miR-139-5p MYB -3.25846663863766 2.62018989415003e-112 1.3385004671291 4.8746375657764e-10 miRanda -0.201268900654479 6.36833621426088e-06 NA NA NA hsa-miR-140-3p MYB -1.25844395244141 5.29222651039892e-33 1.3385004671291 4.8746375657764e-10 miRNATAP -0.703149088566292 2.39987363078617e-26 NA NA NA hsa-miR-140-5p MYB -0.755971041589807 9.68060922934542e-14 1.3385004671291 4.8746375657764e-10 miRanda -0.713881791293094 4.82509990083579e-24 NA NA NA hsa-miR-150-5p MYB -0.832049526091211 3.45299201139224e-05 1.3385004671291 4.8746375657764e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.150452026646376 4.36457888146661e-05 25230975;25522282;23521217;24613688;23675407;22025269 miR 150 functions as a tumour suppressor in human colorectal cancer by targeting c Myb; We report that miR-150 is a key regulator in the tumourigenesis and progression of colorectal cancer by acting as a tumour suppressor targeting c-Myb;The associations of miR-150 c-Myb and MUC4 expression with survival rates were determined; Moreover miR-150 expression was inversely correlated with c-Myb and MUC4 activities in pancreatic tissue cell lines and nude mouse model; miR-150 is an important suppressor of pancreatic ductal carcinoma and acts as a regulator of c-Myb and MUC4 in aggressive progress;Re expression of microRNA 150 induces EBV positive Burkitt lymphoma differentiation by modulating c Myb in vitro; Of note re-expression of miR-150 also resulted in decreasing c-Myb protein levels; Additionally c-Myb knockdown in Daudi and Raji cell lines recapitulated the partial characteristics similar to that caused by re-expression of miR-150; Taken together our findings show that miR-150 can induce EBV-positive BL differentiation by targeting c-Myb;Moreover IHC and western blotting defined that c-Myb and Survivin are the protein targets of miR-150; Deregulation of miR-150 may be useful as a diagnostic tool in BL based on miRNA profile screening qRT-PCR and miRNA-ISH miR-150 plays an important role in BL by targeting c-Myb and Survivin;Furthermore the addition of the miRNA inhibitor 2'-O-methylated miR-150 blocked 3-Cl-AHPC-mediated increase in miR-150* levels and abrogated loss of c-Myb protein;We also show that miR-150 interacts with the 3'UTR of c-Myb mRNA and overexpression of miR-150 downregulates c-Myb protein levels; Collectively our findings demonstrate for the first time that miR-150 may be involved in liver CSC self-renewal potentially via modulation of the downstream target c-Myb progression;poor survival;progression;differentiation;;; colorectal cancer;pancreatic cancer;lymphoma;lymphoma;pancreatic cancer;liver cancer hsa-miR-155-5p MYB 1.09766314984151 4.55650930885814e-15 1.3385004671291 4.8746375657764e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.302949826442919 5.53369049741093e-09 NA NA NA hsa-miR-15a-5p MYB 0.812445324157528 4.50263417099822e-20 1.3385004671291 4.8746375657764e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.295988774508509 0.000337113835483446 NA NA NA hsa-miR-15b-5p MYB 0.788659356634696 1.13678211092534e-11 1.3385004671291 4.8746375657764e-10 miRNATAP -0.329373966819165 1.81773084340454e-07 NA NA NA hsa-miR-16-5p MYB 0.46835991343556 1.91125356547776e-06 1.3385004671291 4.8746375657764e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.353372047227905 2.75374248289707e-06 26934556 The expression levels of two target genes Myb and VEGFR2 were affected significantly by miR-16 while glucose administration inhibited miR-16 expression and enhanced tumor cell proliferation and migration; Hyperglycemia can impact the clinical outcomes of CRC patients likely by inhibiting miR-16 expression and the expression of its downstream genes Myb and VEGFR2 colorectal cancer hsa-miR-215-5p MYB -1.35643065897947 2.3180023440257e-20 1.3385004671291 4.8746375657764e-10 miRNAWalker2_validate -0.32172838281351 6.40521443133396e-11 NA NA NA hsa-miR-217 MYB -0.616728851115349 8.64977119332241e-05 1.3385004671291 4.8746375657764e-10 miRanda -0.191029245435586 5.15472566703123e-05 NA NA NA hsa-miR-28-5p MYB -0.14267073663385 0.0497619246260571 1.3385004671291 4.8746375657764e-10 miRanda -0.793948725008808 6.86876365185527e-15 NA NA NA hsa-miR-320a MYB -0.287921276129346 0.00141693637596294 1.3385004671291 4.8746375657764e-10 miRanda -0.221412212697963 0.00759005866073816 NA NA NA hsa-miR-320b MYB 0.412787504747938 0.00113457276903985 1.3385004671291 4.8746375657764e-10 miRanda -0.218205120332761 0.0002137636171269 NA NA NA hsa-miR-324-3p MYB 0.404877731996915 0.000340036423920834 1.3385004671291 4.8746375657764e-10 PITA -0.269894914817441 4.06305036420134e-05 NA NA NA hsa-miR-335-3p MYB -1.88448538773649 2.09538234398335e-32 1.3385004671291 4.8746375657764e-10 mirMAP -0.170060326447728 0.00015510291704237 NA NA NA hsa-miR-3613-5p MYB 1.11067990676844 4.33424951743633e-21 1.3385004671291 4.8746375657764e-10 miRNATAP -0.239482966833455 0.000105408186917262 NA NA NA hsa-miR-455-5p MYB 0.284641224662773 0.0357572339594277 1.3385004671291 4.8746375657764e-10 miRanda -0.491877875196625 6.52028318541079e-20 NA NA NA hsa-miR-532-3p MYB -0.395473145042459 0.00686714288452761 1.3385004671291 4.8746375657764e-10 miRNATAP -0.509447716854063 7.11169986336984e-25 NA NA NA hsa-miR-590-3p MYB 1.27438512890514 1.03164723481469e-23 1.3385004671291 4.8746375657764e-10 miRanda -0.259709354574183 1.2943032560409e-05 NA NA NA hsa-miR-21-5p MYC 2.32007501846389 3.08394781855554e-137 -1.13124656428776 3.22608205108151e-16 miRNAWalker2_validate -0.270190862069706 5.76701615920235e-10 22072622 In contrast the levels of β-catenin TCF/LEF activity and the expression of c-Myc Cyclin-D which are increased in CSCs are also augmented in miR-21 overexpressing colon cancer cells accompanied by an increased sphere forming ability in vitro and tumor formation in SCID mice colon cancer hsa-miR-34a-5p MYC 0.129201805242403 0.133261654068224 -1.13124656428776 3.22608205108151e-16 miRNAWalker2_validate;miRTarBase -0.216308870548993 0.000129276161670072 25572695;25686834;21460242;22159222;23640973;22830357;22235332 The c-Myc and CD44 were confirmed as direct targets of miR-34a in EJ cell apoptosis induced by PRE;miR 34a induces cellular senescence via modulation of telomerase activity in human hepatocellular carcinoma by targeting FoxM1/c Myc pathway;Myc mediated repression of microRNA 34a promotes high grade transformation of B cell lymphoma by dysregulation of FoxP1;MicroRNA 34a suppresses malignant transformation by targeting c Myc transcriptional complexes in human renal cell carcinoma; We investigated the functional effects of microRNA-34a miR-34a on c-Myc transcriptional complexes in renal cell carcinoma; miR-34a down-regulated expression of multiple oncogenes including c-Myc by targeting its 3' untranslated region which was revealed by luciferase reporter assays; Our results demonstrate that miR-34a suppresses assembly and function of the c-Myc complex that activates or elongates transcription indicating a novel role of miR-34a in the regulation of transcription by c-Myc;Among them miR-34a was also associated with poor prognosis in 2 independent series of leukemic and nodal MCL and in cooperation with high expression of the MYC oncogene;We report that miR-34a did not inhibit cell proliferation notwithstanding a marked down-regulation of c-MYC;MicroRNA 34a modulates c Myc transcriptional complexes to suppress malignancy in human prostate cancer cells; We studied the functional effects of miR-34a on c-Myc transcriptional complexes in PC-3 prostate cancer cells; miR-34a downregulated expression of c-Myc oncogene by targeting its 3' UTR as shown by luciferase reporter assays; This is the first report to document that miR-34a suppresses assembly and function of the c-Myc-Skp2-Miz1 complex that activates RhoA and the c-Myc-pTEFB complex that elongates transcription of various genes suggesting a novel role of miR-34a in the regulation of transcription by c-Myc complex ;;;malignant trasformation;worse prognosis;; bladder cancer;liver cancer;B cell lymphoma;kidney renal cell cancer;lymphoma;lymphoma;prostate cancer hsa-miR-429 MYC 2.84108892259147 3.88520896426515e-64 -1.13124656428776 3.22608205108151e-16 miRNAWalker2_validate -0.108453248703558 4.76591679996824e-05 21684154;24633485 miR 429 modulates the expression of c myc in human gastric carcinoma cells; SGC-7901 gastric cancer cells were transfected with miR-429 mimics and endogenous c-myc expression was detected by western blots; We performed functional assays using the 3'UTR of the c-myc gene as a miR-429 target in a luciferase reporter assay system; miR-429 significantly downregulated endogenous c-myc expression in SGC-7901 cells; Action of miR/429 on c-myc 3'UTR was confirmed; c-myc is an important miR-429 target gene;It is known that miR-429 is down-regulated and functions as a tumor suppressor by targeting c-myc and PLGG1 in gastric and breast cancer ; gastric cancer;sarcoma hsa-let-7d-5p NGF 0.410958462607207 6.07808182756787e-06 -0.787725123054401 9.35669050853888e-08 MirTarget -0.171326999284145 0.00225973911147285 NA NA NA hsa-miR-429 NGF 2.84108892259147 3.88520896426515e-64 -0.787725123054401 9.35669050853888e-08 miRanda -0.12206490353919 1.36541405382157e-05 NA NA NA hsa-miR-15b-3p NGFR 1.12898579025388 1.18050912917317e-14 -2.50042294481334 5.32154099004444e-27 mirMAP -0.662442871026151 9.19146915678377e-36 NA NA NA hsa-miR-185-3p NGFR 0.86591374114446 7.8790769678542e-10 -2.50042294481334 5.32154099004444e-27 miRNATAP -0.558631835952441 2.12953197650633e-22 NA NA NA hsa-miR-193a-3p NGFR 0.310617999804917 0.00477640270974957 -2.50042294481334 5.32154099004444e-27 miRanda -0.520708123872055 1.99092128302359e-12 NA NA NA hsa-miR-27a-3p NGFR 0.388496361379095 1.42373532086331e-05 -2.50042294481334 5.32154099004444e-27 miRNATAP -0.439062106500076 1.68928439077992e-06 NA NA NA hsa-miR-30b-3p NGFR -0.536462824497982 2.41814659272548e-05 -2.50042294481334 5.32154099004444e-27 mirMAP -0.305652864637524 2.34744572510331e-06 NA NA NA hsa-miR-324-5p NGFR 0.211552647440779 0.139408130311654 -2.50042294481334 5.32154099004444e-27 miRanda -0.552743690537487 6.69210223218312e-23 NA NA NA hsa-miR-330-5p NGFR 0.626063225953928 3.42215028606779e-09 -2.50042294481334 5.32154099004444e-27 miRanda;mirMAP;miRNATAP -0.663548277903429 1.91996593924222e-18 NA NA NA hsa-miR-342-5p NGFR 1.49554286219821 1.56221300255782e-22 -2.50042294481334 5.32154099004444e-27 mirMAP -0.37697123789083 2.96787913045429e-13 NA NA NA hsa-miR-34a-5p NGFR 0.129201805242403 0.133261654068224 -2.50042294481334 5.32154099004444e-27 mirMAP -0.493588136203429 2.5977817562076e-07 NA NA NA hsa-miR-429 NGFR 2.84108892259147 3.88520896426515e-64 -2.50042294481334 5.32154099004444e-27 miRNATAP -0.365440513479821 3.03625910927681e-16 NA NA NA hsa-miR-625-5p NGFR 0.728939236913178 5.97591217674307e-08 -2.50042294481334 5.32154099004444e-27 mirMAP -0.290398887460611 3.44867089710813e-06 NA NA NA hsa-miR-766-3p NGFR 0.496790679032227 8.22686414022553e-05 -2.50042294481334 5.32154099004444e-27 mirMAP -0.45318423386739 1.88340574844031e-12 NA NA NA hsa-miR-429 NOS3 2.84108892259147 3.88520896426515e-64 -0.415857403289588 4.78627333883668e-05 miRanda -0.130650037890721 1.06798855882441e-11 NA NA NA hsa-let-7d-5p NR4A1 0.410958462607207 6.07808182756787e-06 -1.94844386566107 4.44471295400257e-27 miRNAWalker2_validate -0.392348094917098 2.16168423920223e-08 NA NA NA hsa-miR-324-5p NR4A1 0.211552647440779 0.139408130311654 -1.94844386566107 4.44471295400257e-27 miRanda -0.316675057081773 8.15079425391408e-13 NA NA NA hsa-miR-93-5p NR4A1 1.01788600209314 1.24312921618126e-21 -1.94844386566107 4.44471295400257e-27 miRNAWalker2_validate -0.261546738240957 8.05513957986116e-06 NA NA NA hsa-miR-26a-5p NRAS -0.481180084102235 1.82368246174758e-10 0.183758021967058 0.0269071454303856 mirMAP;miRNATAP -0.171830491303475 4.57786379954293e-06 26458859 MiR 26a enhances the sensitivity of gastric cancer cells to cisplatin by targeting NRAS and E2F2; Our results suggest that miR-26a can improve the sensitivity of GC cells to cisplatin-based chemotherapies through targeting NRAS and E2F2 and provide the first evidence of the potential utility of miR-26a as a sensitizer in chemotherapy for GC gastric cancer hsa-miR-29a-3p NRAS -0.699539666833076 1.13935003139957e-13 0.183758021967058 0.0269071454303856 miRNATAP -0.131093968344875 1.07589675767432e-05 NA NA NA hsa-miR-29c-3p NRAS -0.0652732378039769 0.563774115756922 0.183758021967058 0.0269071454303856 miRNATAP -0.211489064949879 1.38049039033829e-17 NA NA NA hsa-miR-381-3p NRAS -1.28028930293369 1.61402949866141e-23 0.183758021967058 0.0269071454303856 mirMAP -0.110038347690747 3.16999123322707e-07 NA NA NA hsa-miR-142-3p NTF3 1.53951237846104 2.06769936282034e-17 -2.35193065681614 1.75397805257351e-24 miRanda -0.25559087299935 4.55546500058392e-09 NA NA NA hsa-miR-200b-3p NTF3 2.11744072703209 1.66710572104191e-43 -2.35193065681614 1.75397805257351e-24 TargetScan -0.321874947951398 1.05323715646676e-10 NA NA NA hsa-miR-200c-3p NTF3 2.07038651806183 6.84613386286599e-51 -2.35193065681614 1.75397805257351e-24 miRNAWalker2_validate;miRNATAP -0.405535845133949 1.08735324650309e-13 NA NA NA hsa-miR-21-5p NTF3 2.32007501846389 3.08394781855554e-137 -2.35193065681614 1.75397805257351e-24 miRTarBase;miRNATAP -0.481266108265449 4.86045305948914e-11 NA NA NA hsa-miR-429 NTF3 2.84108892259147 3.88520896426515e-64 -2.35193065681614 1.75397805257351e-24 PITA;miRanda;miRNATAP -0.318352061640753 7.25173081695566e-13 NA NA NA hsa-miR-590-3p NTF3 1.27438512890514 1.03164723481469e-23 -2.35193065681614 1.75397805257351e-24 miRanda -0.327104104078733 4.60676593188325e-07 NA NA NA hsa-miR-590-5p NTF3 0.746515607868274 8.51300479640125e-09 -2.35193065681614 1.75397805257351e-24 PITA;miRanda;miRNATAP -0.249639896667382 5.92923109438771e-05 NA NA NA hsa-miR-107 NTRK2 0.678998026350662 2.56224148930538e-16 -4.03565225718056 9.2986992242195e-64 PITA;miRanda -0.836390405916991 1.12926053720617e-15 NA NA NA hsa-miR-130b-3p NTRK2 1.38122163823846 1.00932179737836e-26 -4.03565225718056 9.2986992242195e-64 mirMAP -0.769347321078169 1.17967706482035e-32 NA NA NA hsa-miR-130b-5p NTRK2 1.0573161199584 2.55177349228739e-12 -4.03565225718056 9.2986992242195e-64 mirMAP -0.488025092950443 1.17958520925326e-17 NA NA NA hsa-miR-142-3p NTRK2 1.53951237846104 2.06769936282034e-17 -4.03565225718056 9.2986992242195e-64 miRanda -0.311735989864694 5.64944316358857e-11 NA NA NA hsa-miR-142-5p NTRK2 1.16509303143593 1.96641162209073e-13 -4.03565225718056 9.2986992242195e-64 mirMAP -0.267780450784351 1.15450126012637e-06 NA NA NA hsa-miR-146b-5p NTRK2 0.631041661167954 6.20366593452965e-08 -4.03565225718056 9.2986992242195e-64 PITA;miRanda -0.314474616023657 3.27685992622639e-05 NA NA NA hsa-miR-148a-5p NTRK2 0.572692271754695 2.14306251522796e-06 -4.03565225718056 9.2986992242195e-64 mirMAP -0.198050799697867 0.00696218497554125 NA NA NA hsa-miR-151a-5p NTRK2 0.509210376738198 3.17540030607215e-07 -4.03565225718056 9.2986992242195e-64 miRNATAP -0.780564562612712 3.70349058685946e-19 NA NA NA hsa-miR-155-5p NTRK2 1.09766314984151 4.55650930885814e-15 -4.03565225718056 9.2986992242195e-64 mirMAP -0.41539155563853 1.85377330452013e-11 NA NA NA hsa-miR-15b-3p NTRK2 1.12898579025388 1.18050912917317e-14 -4.03565225718056 9.2986992242195e-64 mirMAP -0.562890529553338 5.4599239852998e-22 NA NA NA hsa-miR-17-3p NTRK2 0.721439431208839 7.95900548819742e-10 -4.03565225718056 9.2986992242195e-64 miRNATAP -0.45111098908225 1.45919732139291e-09 NA NA NA hsa-miR-181a-5p NTRK2 0.635863607630183 6.72486918157744e-11 -4.03565225718056 9.2986992242195e-64 mirMAP -0.680395100851069 2.6766742709822e-14 NA NA NA hsa-miR-181b-5p NTRK2 1.22749257795094 1.21998198830919e-27 -4.03565225718056 9.2986992242195e-64 mirMAP -0.779842929679368 9.18504195603619e-26 NA NA NA hsa-miR-182-5p NTRK2 2.36408084103818 2.60011457600281e-72 -4.03565225718056 9.2986992242195e-64 mirMAP -0.570548474380832 3.49801193738836e-21 NA NA NA hsa-miR-185-5p NTRK2 0.482334746589337 3.68475879959415e-09 -4.03565225718056 9.2986992242195e-64 mirMAP -0.870491482821692 3.554861973461e-16 NA NA NA hsa-miR-200c-3p NTRK2 2.07038651806183 6.84613386286599e-51 -4.03565225718056 9.2986992242195e-64 miRNAWalker2_validate;miRTarBase -0.540459062838814 7.12630091174492e-20 23209748;23074172 miR 200c sensitizes breast cancer cells to doxorubicin treatment by decreasing TrkB and Bmi1 expression;miR-200c also targets TrkB a mediator of resistance to anoikis ;drug resistance breast cancer;ovarian cancer hsa-miR-21-5p NTRK2 2.32007501846389 3.08394781855554e-137 -4.03565225718056 9.2986992242195e-64 mirMAP -1.20592530757402 1.06429280476928e-57 NA NA NA hsa-miR-27b-5p NTRK2 0.478244809913032 2.28657237923291e-06 -4.03565225718056 9.2986992242195e-64 mirMAP -0.459936572788305 1.37095676083519e-07 NA NA NA hsa-miR-301a-3p NTRK2 1.32170168375632 2.12280368957514e-15 -4.03565225718056 9.2986992242195e-64 mirMAP -0.591414503280674 3.56317078452758e-31 NA NA NA hsa-miR-32-5p NTRK2 1.38676603945192 2.29428299685976e-34 -4.03565225718056 9.2986992242195e-64 mirMAP -0.661395204008918 3.13696590151528e-19 NA NA NA hsa-miR-330-5p NTRK2 0.626063225953928 3.42215028606779e-09 -4.03565225718056 9.2986992242195e-64 miRanda -0.478715290991117 7.66297627939398e-09 NA NA NA hsa-miR-33a-5p NTRK2 1.35185127289399 9.80509199125785e-12 -4.03565225718056 9.2986992242195e-64 mirMAP -0.330110530234138 1.17990045018443e-13 NA NA NA hsa-miR-340-5p NTRK2 1.0857159627596 4.94471062546756e-24 -4.03565225718056 9.2986992242195e-64 mirMAP -0.658186289275308 1.12023703246196e-16 NA NA NA hsa-miR-342-3p NTRK2 1.21944481975657 6.81914373001202e-19 -4.03565225718056 9.2986992242195e-64 miRanda -0.259575565229622 4.07249780461087e-05 NA NA NA hsa-miR-362-5p NTRK2 -0.248676340901797 0.0726089851456282 -4.03565225718056 9.2986992242195e-64 mirMAP -0.211021259711251 0.00102124609335382 NA NA NA hsa-miR-429 NTRK2 2.84108892259147 3.88520896426515e-64 -4.03565225718056 9.2986992242195e-64 PITA;miRanda;miRNATAP -0.51698534306774 1.82175500468262e-27 NA NA NA hsa-miR-454-3p NTRK2 1.28177672191095 6.84024979484587e-21 -4.03565225718056 9.2986992242195e-64 mirMAP -0.612915092365335 8.08891527812014e-23 NA NA NA hsa-miR-455-3p NTRK2 0.678392920628872 0.000327758844555877 -4.03565225718056 9.2986992242195e-64 mirMAP -0.199163639140476 2.0930591380016e-05 NA NA NA hsa-miR-532-5p NTRK2 0.0386976222860582 0.682018574136515 -4.03565225718056 9.2986992242195e-64 PITA -0.521210748289278 3.09260110111958e-08 NA NA NA hsa-miR-576-5p NTRK2 0.0649387707567879 0.588791966096287 -4.03565225718056 9.2986992242195e-64 PITA;mirMAP -0.278348766635835 0.000174544446483956 NA NA NA hsa-miR-590-3p NTRK2 1.27438512890514 1.03164723481469e-23 -4.03565225718056 9.2986992242195e-64 PITA;miRanda;mirMAP -0.630708695964853 1.65227664250245e-19 NA NA NA hsa-miR-590-5p NTRK2 0.746515607868274 8.51300479640125e-09 -4.03565225718056 9.2986992242195e-64 miRanda;mirMAP -0.533409974311982 1.82799161345103e-15 NA NA NA hsa-miR-193a-3p OSMR 0.310617999804917 0.00477640270974957 -0.668362253183246 2.80577564076125e-06 MirTarget -0.129820817377299 0.00371242521618259 NA NA NA hsa-miR-200b-3p OSMR 2.11744072703209 1.66710572104191e-43 -0.668362253183246 2.80577564076125e-06 TargetScan -0.20408879563362 1.18112518586047e-11 NA NA NA hsa-miR-429 OSMR 2.84108892259147 3.88520896426515e-64 -0.668362253183246 2.80577564076125e-06 miRanda;miRNATAP -0.181544786242502 1.42143787332253e-11 NA NA NA hsa-miR-590-3p OSMR 1.27438512890514 1.03164723481469e-23 -0.668362253183246 2.80577564076125e-06 miRanda;mirMAP;miRNATAP -0.126738008266365 0.0012960013841292 NA NA NA hsa-miR-98-5p OSMR 0.656336869298973 9.03113188590466e-15 -0.668362253183246 2.80577564076125e-06 miRNAWalker2_validate -0.259591236387239 5.89309442597424e-06 NA NA NA hsa-miR-590-3p PCK1 1.27438512890514 1.03164723481469e-23 -6.28805678682684 1.41732381208289e-58 miRanda -0.937752859089419 1.28924493149732e-16 NA NA NA hsa-miR-24-3p PCK2 -0.120123317289941 0.21432376743243 0.663748316053731 2.32985369690914e-13 miRNAWalker2_validate -0.148569750676668 5.1712568901566e-06 NA NA NA hsa-let-7d-5p PDGFA 0.410958462607207 6.07808182756787e-06 -1.03194821362155 2.98319775634862e-27 miRNAWalker2_validate -0.111085037882515 0.0028367772618075 NA NA NA hsa-miR-331-5p PDGFA 0.682437632502949 4.10871642662371e-11 -1.03194821362155 2.98319775634862e-27 PITA;miRNATAP -0.22667510028007 1.7281960179029e-12 NA NA NA hsa-miR-484 PDGFA 0.349444060004737 0.00154491249625173 -1.03194821362155 2.98319775634862e-27 PITA;miRNATAP -0.214059857890823 1.54568697231701e-12 NA NA NA hsa-miR-590-3p PDGFA 1.27438512890514 1.03164723481469e-23 -1.03194821362155 2.98319775634862e-27 miRanda -0.165100330465473 7.8988455074446e-10 NA NA NA hsa-miR-590-5p PDGFA 0.746515607868274 8.51300479640125e-09 -1.03194821362155 2.98319775634862e-27 miRanda -0.119998279179232 3.27111011320567e-06 NA NA NA hsa-let-7d-5p PDGFB 0.410958462607207 6.07808182756787e-06 0.0445377066563912 0.601024636333503 miRNATAP -0.127460924180761 6.8859001946812e-05 NA NA NA hsa-let-7g-5p PDGFB 0.0200025784917734 0.791635298815256 0.0445377066563912 0.601024636333503 miRNATAP -0.148185313332358 0.000134872450400757 NA NA NA hsa-let-7i-5p PDGFB -0.333419326258177 3.56960810786801e-08 0.0445377066563912 0.601024636333503 miRNATAP -0.131426457663067 0.00682130071674743 NA NA NA hsa-miR-146b-3p PDGFB -0.260891807886908 0.0211838235241739 0.0445377066563912 0.601024636333503 miRNAWalker2_validate -0.113394049081063 1.0320398497476e-05 NA NA NA hsa-miR-17-5p PDGFB 0.599531757298529 1.07318660415906e-05 0.0445377066563912 0.601024636333503 TargetScan -0.107407253083859 4.08751757932673e-07 NA NA NA hsa-miR-29a-3p PDGFB -0.699539666833076 1.13935003139957e-13 0.0445377066563912 0.601024636333503 MirTarget;miRNATAP -0.128475198337676 2.5654295496112e-05 NA NA NA hsa-let-7a-3p PDGFC 0.466622567891891 3.70886257943079e-07 -0.978440727200944 8.9591213400997e-19 mirMAP -0.130143500695485 0.00200973229404423 NA NA NA hsa-miR-142-5p PDGFC 1.16509303143593 1.96641162209073e-13 -0.978440727200944 8.9591213400997e-19 PITA -0.16246211089026 8.90808371952716e-12 NA NA NA hsa-miR-16-2-3p PDGFC 0.286355254959005 0.0256946046884474 -0.978440727200944 8.9591213400997e-19 mirMAP -0.200678062147313 2.53333168647401e-11 NA NA NA hsa-miR-330-3p PDGFC 0.432728362037032 0.00041335506960812 -0.978440727200944 8.9591213400997e-19 mirMAP -0.203742285211782 1.32009888968262e-10 NA NA NA hsa-miR-330-5p PDGFC 0.626063225953928 3.42215028606779e-09 -0.978440727200944 8.9591213400997e-19 miRanda -0.252272874306445 2.15422319075801e-12 NA NA NA hsa-miR-340-5p PDGFC 1.0857159627596 4.94471062546756e-24 -0.978440727200944 8.9591213400997e-19 mirMAP -0.314553890954425 6.17997923484242e-20 NA NA NA hsa-miR-361-5p PDGFC -0.0996867547900457 0.095428250126326 -0.978440727200944 8.9591213400997e-19 miRanda -0.318946471741077 1.16865088583823e-06 NA NA NA hsa-miR-374a-3p PDGFC 0.34122393953022 2.28714105382856e-05 -0.978440727200944 8.9591213400997e-19 mirMAP -0.226510946292756 2.43085641584885e-06 NA NA NA hsa-miR-590-3p PDGFC 1.27438512890514 1.03164723481469e-23 -0.978440727200944 8.9591213400997e-19 PITA;miRanda;mirMAP -0.277327179688994 6.90970581985756e-20 NA NA NA hsa-miR-7-1-3p PDGFC 0.686579567507582 1.20731554448806e-07 -0.978440727200944 8.9591213400997e-19 mirMAP -0.116839921632248 7.99328092215278e-05 NA NA NA hsa-miR-125a-3p PDGFD -0.176412960953959 0.10108079286419 -2.17788738091168 7.06207148591019e-60 miRanda -0.119583380809276 0.00952184976958956 NA NA NA hsa-miR-19b-1-5p PDGFD 0.647704962003062 3.07687667945597e-07 -2.17788738091168 7.06207148591019e-60 MirTarget -0.438124514443653 9.84221523032561e-32 NA NA NA hsa-miR-203a-3p PDGFD 1.67084394806148 6.06522273352132e-14 -2.17788738091168 7.06207148591019e-60 MirTarget -0.178881432965936 6.3979976925553e-17 NA NA NA hsa-miR-21-5p PDGFD 2.32007501846389 3.08394781855554e-137 -2.17788738091168 7.06207148591019e-60 miRNAWalker2_validate -0.58246612445279 1.38968424696146e-42 NA NA NA hsa-miR-320b PDGFD 0.412787504747938 0.00113457276903985 -2.17788738091168 7.06207148591019e-60 miRanda -0.234603273416289 1.42000791647672e-09 NA NA NA hsa-miR-330-3p PDGFD 0.432728362037032 0.00041335506960812 -2.17788738091168 7.06207148591019e-60 MirTarget;PITA -0.186433075255229 4.45712214549411e-06 NA NA NA hsa-miR-331-5p PDGFD 0.682437632502949 4.10871642662371e-11 -2.17788738091168 7.06207148591019e-60 MirTarget -0.44396123572881 9.47659812594788e-22 NA NA NA hsa-miR-590-3p PDGFD 1.27438512890514 1.03164723481469e-23 -2.17788738091168 7.06207148591019e-60 miRanda;mirMAP;miRNATAP -0.499606568060295 3.79285218268692e-40 NA NA NA hsa-miR-590-5p PDGFD 0.746515607868274 8.51300479640125e-09 -2.17788738091168 7.06207148591019e-60 miRanda -0.454069225558139 4.11350350024471e-36 NA NA NA hsa-miR-7-1-3p PDGFD 0.686579567507582 1.20731554448806e-07 -2.17788738091168 7.06207148591019e-60 mirMAP -0.179504767545686 1.85513561254515e-06 NA NA NA hsa-miR-9-5p PDGFD 0.734772328078424 0.00261982843636383 -2.17788738091168 7.06207148591019e-60 MirTarget -0.16906539909882 1.1572662105982e-17 NA NA NA hsa-let-7a-3p PDGFRA 0.466622567891891 3.70886257943079e-07 -1.84169721584144 2.50038285124215e-31 mirMAP -0.329460092115081 6.44888590147222e-08 NA NA NA hsa-miR-106a-5p PDGFRA 0.574594373206545 0.000145868284001732 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.114055302211927 0.0021406545639755 NA NA NA hsa-miR-106b-5p PDGFRA 1.22477789913175 2.31787213143326e-32 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.488023399387153 1.85919087848084e-21 NA NA NA hsa-miR-130b-3p PDGFRA 1.38122163823846 1.00932179737836e-26 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.205861486268687 1.18866735754345e-06 NA NA NA hsa-miR-141-3p PDGFRA 2.99777540204168 1.26370498493024e-78 -1.84169721584144 2.50038285124215e-31 MirTarget -0.465703815469129 7.78017639246501e-55 NA NA NA hsa-miR-149-5p PDGFRA 0.218233101703391 0.261629158371175 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.10186586746034 0.000432641759044271 NA NA NA hsa-miR-16-1-3p PDGFRA 1.30142784811196 2.53343713753744e-29 -1.84169721584144 2.50038285124215e-31 MirTarget -0.445034147047717 7.48526207581866e-20 NA NA NA hsa-miR-17-5p PDGFRA 0.599531757298529 1.07318660415906e-05 -1.84169721584144 2.50038285124215e-31 MirTarget;TargetScan;miRNATAP -0.230385311598016 1.83020228188668e-08 NA NA NA hsa-miR-181b-5p PDGFRA 1.22749257795094 1.21998198830919e-27 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.19733318786805 4.51362347726677e-05 NA NA NA hsa-miR-181c-5p PDGFRA 0.574847839298605 4.51287502066478e-07 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.199505884862497 4.93594812999378e-05 NA NA NA hsa-miR-181d-5p PDGFRA 0.782618466877349 1.2821116358212e-09 -1.84169721584144 2.50038285124215e-31 MirTarget -0.209000657310327 1.2726595161397e-06 NA NA NA hsa-miR-182-5p PDGFRA 2.36408084103818 2.60011457600281e-72 -1.84169721584144 2.50038285124215e-31 miRNATAP -0.488133036134936 5.32981617385656e-39 NA NA NA hsa-miR-193a-3p PDGFRA 0.310617999804917 0.00477640270974957 -1.84169721584144 2.50038285124215e-31 miRanda -0.236933469215575 3.5076412689528e-06 NA NA NA hsa-miR-200a-3p PDGFRA 2.55218480823434 2.96135446816238e-51 -1.84169721584144 2.50038285124215e-31 MirTarget -0.404359885272289 5.76229938736958e-42 NA NA NA hsa-miR-20a-5p PDGFRA 0.44979911120222 0.000602386964327228 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.252447130222501 3.08338747587722e-09 NA NA NA hsa-miR-20b-5p PDGFRA 0.78236583455125 8.7938334801223e-05 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.120554127383801 1.73060256254725e-05 NA NA NA hsa-miR-29b-2-5p PDGFRA -0.263499971180298 0.0400001755611965 -1.84169721584144 2.50038285124215e-31 mirMAP -0.308292211262466 1.26968663702118e-12 NA NA NA hsa-miR-29b-3p PDGFRA 0.438847962603729 6.46722433236121e-05 -1.84169721584144 2.50038285124215e-31 MirTarget -0.500389516534886 1.15065170563473e-23 NA NA NA hsa-miR-29c-3p PDGFRA -0.0652732378039769 0.563774115756922 -1.84169721584144 2.50038285124215e-31 MirTarget -0.350109088283135 1.37340184176404e-12 NA NA NA hsa-miR-301a-3p PDGFRA 1.32170168375632 2.12280368957514e-15 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.255990348992222 8.11143570915912e-15 NA NA NA hsa-miR-320b PDGFRA 0.412787504747938 0.00113457276903985 -1.84169721584144 2.50038285124215e-31 miRanda -0.183733564050286 3.69207458785768e-05 NA NA NA hsa-miR-330-5p PDGFRA 0.626063225953928 3.42215028606779e-09 -1.84169721584144 2.50038285124215e-31 miRanda -0.192776801359275 0.000261739400977773 NA NA NA hsa-miR-338-3p PDGFRA 0.0799903526884966 0.575137668326679 -1.84169721584144 2.50038285124215e-31 miRanda -0.319924077772208 1.84779948590881e-16 NA NA NA hsa-miR-339-5p PDGFRA -0.211548290465418 0.0685885445822389 -1.84169721584144 2.50038285124215e-31 miRanda -0.18956471906179 9.38243938310758e-05 NA NA NA hsa-miR-33a-5p PDGFRA 1.35185127289399 9.80509199125785e-12 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.153487879078261 6.47868493614725e-08 NA NA NA hsa-miR-342-3p PDGFRA 1.21944481975657 6.81914373001202e-19 -1.84169721584144 2.50038285124215e-31 MirTarget;miRanda;miRNATAP -0.370862046281642 3.2391388940783e-21 NA NA NA hsa-miR-34a-5p PDGFRA 0.129201805242403 0.133261654068224 -1.84169721584144 2.50038285124215e-31 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.257308196495663 9.23678964579895e-05 22750848 Mechanistically in addition to its direct regulation of platelet-derived growth factor receptor-alpha PDGFRA promoter enrichment analysis of context likelihood of relatedness-inferred mRNA nodes established miR-34a as a novel regulator of a SMAD4 transcriptional network glioblastoma hsa-miR-429 PDGFRA 2.84108892259147 3.88520896426515e-64 -1.84169721584144 2.50038285124215e-31 miRanda;miRNATAP -0.373691764862624 4.97896969778458e-36 NA NA NA hsa-miR-454-3p PDGFRA 1.28177672191095 6.84024979484587e-21 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.35092972448673 9.46773059007511e-19 NA NA NA hsa-miR-590-3p PDGFRA 1.27438512890514 1.03164723481469e-23 -1.84169721584144 2.50038285124215e-31 miRanda;mirMAP -0.264364229545702 3.69842178603011e-09 NA NA NA hsa-miR-590-5p PDGFRA 0.746515607868274 8.51300479640125e-09 -1.84169721584144 2.50038285124215e-31 miRanda -0.212109048295796 7.97276608700393e-07 NA NA NA hsa-miR-664a-3p PDGFRA -0.237699857923843 0.0197071390183375 -1.84169721584144 2.50038285124215e-31 mirMAP -0.180157525965088 0.00115407518602231 NA NA NA hsa-miR-7-1-3p PDGFRA 0.686579567507582 1.20731554448806e-07 -1.84169721584144 2.50038285124215e-31 mirMAP -0.307956291240907 5.06202621843896e-13 NA NA NA hsa-miR-93-5p PDGFRA 1.01788600209314 1.24312921618126e-21 -1.84169721584144 2.50038285124215e-31 MirTarget;miRNATAP -0.499449521835939 3.61616920956413e-23 NA NA NA hsa-miR-96-5p PDGFRA 3.24596558166606 1.92296159041707e-98 -1.84169721584144 2.50038285124215e-31 TargetScan;miRNATAP -0.477431215706839 8.10828261241382e-56 NA NA NA hsa-miR-29a-3p PDGFRB -0.699539666833076 1.13935003139957e-13 -0.363053423609744 0.000930448280520273 miRNATAP -0.173489965488495 1.08203138499797e-05 NA NA NA hsa-miR-29b-3p PDGFRB 0.438847962603729 6.46722433236121e-05 -0.363053423609744 0.000930448280520273 miRNATAP -0.37404884670886 1.21864334087282e-29 NA NA NA hsa-miR-29c-3p PDGFRB -0.0652732378039769 0.563774115756922 -0.363053423609744 0.000930448280520273 miRNATAP -0.131693600188518 7.8570505856864e-05 NA NA NA hsa-miR-3127-5p PDGFRB 0.903405128081314 1.1325923598273e-12 -0.363053423609744 0.000930448280520273 mirMAP -0.190856341471899 4.71469241759899e-11 NA NA NA hsa-miR-338-3p PDGFRB 0.0799903526884966 0.575137668326679 -0.363053423609744 0.000930448280520273 miRanda -0.135507787608143 2.53645768220537e-07 NA NA NA hsa-miR-342-3p PDGFRB 1.21944481975657 6.81914373001202e-19 -0.363053423609744 0.000930448280520273 miRanda -0.120554527834238 6.56852598546147e-06 NA NA NA hsa-miR-361-3p PDGFRB -0.0370446538644886 0.624902392829872 -0.363053423609744 0.000930448280520273 MirTarget -0.332024100699143 2.34711270011442e-11 NA NA NA hsa-miR-484 PDGFRB 0.349444060004737 0.00154491249625173 -0.363053423609744 0.000930448280520273 mirMAP -0.17421282482312 2.9286066688125e-07 NA NA NA hsa-miR-664a-3p PDGFRB -0.237699857923843 0.0197071390183375 -0.363053423609744 0.000930448280520273 MirTarget -0.173222382702463 2.70287047774181e-06 NA NA NA hsa-miR-103a-3p PDPK1 0.610673390034345 1.63113920514646e-12 -0.0553897078043679 0.47893230919387 mirMAP -0.195227401446081 1.40474326687401e-10 NA NA NA hsa-miR-106b-5p PDPK1 1.22477789913175 2.31787213143326e-32 -0.0553897078043679 0.47893230919387 mirMAP -0.188998401205378 1.5003883981558e-14 NA NA NA hsa-miR-107 PDPK1 0.678998026350662 2.56224148930538e-16 -0.0553897078043679 0.47893230919387 mirMAP -0.159185203283762 5.38122436376593e-07 NA NA NA hsa-miR-128-3p PDPK1 0.90296715955238 9.925204823863e-23 -0.0553897078043679 0.47893230919387 miRNAWalker2_validate -0.176951960154388 3.45376583543913e-10 NA NA NA hsa-miR-15a-5p PDPK1 0.812445324157528 4.50263417099822e-20 -0.0553897078043679 0.47893230919387 mirMAP -0.221903804018165 3.37728999804261e-14 NA NA NA hsa-miR-17-5p PDPK1 0.599531757298529 1.07318660415906e-05 -0.0553897078043679 0.47893230919387 miRNAWalker2_validate;mirMAP -0.118251463530882 1.11170801086741e-09 NA NA NA hsa-miR-185-5p PDPK1 0.482334746589337 3.68475879959415e-09 -0.0553897078043679 0.47893230919387 miRNAWalker2_validate -0.209025442029251 1.03227784292795e-10 NA NA NA hsa-miR-20a-5p PDPK1 0.44979911120222 0.000602386964327228 -0.0553897078043679 0.47893230919387 mirMAP -0.187876784201181 3.83298842174516e-21 NA NA NA hsa-miR-22-5p PDPK1 0.238328801322329 0.0377496760295305 -0.0553897078043679 0.47893230919387 miRNATAP -0.103270556943386 9.3434029051065e-06 NA NA NA hsa-miR-24-3p PDPK1 -0.120123317289941 0.21432376743243 -0.0553897078043679 0.47893230919387 miRNAWalker2_validate -0.112573425800778 4.9504814395065e-05 NA NA NA hsa-miR-27a-3p PDPK1 0.388496361379095 1.42373532086331e-05 -0.0553897078043679 0.47893230919387 miRNATAP -0.166657339522545 2.08058309346529e-08 NA NA NA hsa-miR-27b-3p PDPK1 -0.0796803270437447 0.410503155574393 -0.0553897078043679 0.47893230919387 miRNATAP -0.128280079054786 3.59271031339703e-06 NA NA NA hsa-miR-28-5p PDPK1 -0.14267073663385 0.0497619246260571 -0.0553897078043679 0.47893230919387 mirMAP -0.188098133932334 3.6777139194769e-07 NA NA NA hsa-miR-29a-3p PDPK1 -0.699539666833076 1.13935003139957e-13 -0.0553897078043679 0.47893230919387 mirMAP -0.123115773524495 1.11259237928009e-05 NA NA NA hsa-miR-361-5p PDPK1 -0.0996867547900457 0.095428250126326 -0.0553897078043679 0.47893230919387 miRanda;mirMAP -0.395197723069709 8.02735285255607e-19 NA NA NA hsa-miR-374a-3p PDPK1 0.34122393953022 2.28714105382856e-05 -0.0553897078043679 0.47893230919387 mirMAP -0.151612379692758 4.98877746993602e-06 NA NA NA hsa-miR-374b-5p PDPK1 -0.466781429946153 1.17238311619116e-08 -0.0553897078043679 0.47893230919387 miRNATAP -0.173756085269167 8.73605181551714e-08 NA NA NA hsa-miR-542-3p PDPK1 0.568822103820889 8.5699075257651e-07 -0.0553897078043679 0.47893230919387 mirMAP -0.121087390005675 1.29773467969966e-07 NA NA NA hsa-miR-93-5p PDPK1 1.01788600209314 1.24312921618126e-21 -0.0553897078043679 0.47893230919387 mirMAP -0.192079485583165 1.90008080577296e-15 NA NA NA hsa-miR-142-3p PGF 1.53951237846104 2.06769936282034e-17 0.187603779609442 0.0921943717527187 miRanda -0.110153556627631 7.64442465753915e-08 NA NA NA hsa-miR-326 PGF -1.54449030848141 1.04703417845594e-17 0.187603779609442 0.0921943717527187 mirMAP -0.12535880272214 1.12977476405779e-09 NA NA NA hsa-miR-330-5p PGF 0.626063225953928 3.42215028606779e-09 0.187603779609442 0.0921943717527187 mirMAP -0.169384385810917 2.04633751679976e-06 NA NA NA hsa-miR-766-3p PGF 0.496790679032227 8.22686414022553e-05 0.187603779609442 0.0921943717527187 MirTarget -0.149878694548497 6.09352519262623e-07 NA NA NA hsa-miR-589-5p PHLPP1 0.367182541239722 1.67804815542873e-05 -0.769928393860824 2.16724047885379e-17 MirTarget -0.121857311491064 0.0010647852689542 NA NA NA hsa-miR-106b-5p PHLPP2 1.22477789913175 2.31787213143326e-32 -0.383642052753594 7.63863404919517e-07 miRNATAP -0.123562997753584 6.31243236342397e-07 NA NA NA hsa-miR-15a-5p PHLPP2 0.812445324157528 4.50263417099822e-20 -0.383642052753594 7.63863404919517e-07 MirTarget;miRNATAP -0.223528601903918 2.01431935237275e-14 NA NA NA hsa-miR-185-5p PHLPP2 0.482334746589337 3.68475879959415e-09 -0.383642052753594 7.63863404919517e-07 MirTarget;miRNATAP -0.1543161954068 2.0396401968705e-06 NA NA NA hsa-miR-20a-5p PHLPP2 0.44979911120222 0.000602386964327228 -0.383642052753594 7.63863404919517e-07 miRNATAP -0.128142034012742 2.11074236230527e-10 NA NA NA hsa-miR-26a-5p PHLPP2 -0.481180084102235 1.82368246174758e-10 -0.383642052753594 7.63863404919517e-07 mirMAP -0.166869075056111 2.14596603045617e-06 NA NA NA hsa-miR-27a-3p PHLPP2 0.388496361379095 1.42373532086331e-05 -0.383642052753594 7.63863404919517e-07 MirTarget;miRNATAP -0.186944436167634 2.78304427050395e-10 NA NA NA hsa-miR-27b-3p PHLPP2 -0.0796803270437447 0.410503155574393 -0.383642052753594 7.63863404919517e-07 MirTarget;miRNATAP -0.174530450922766 2.19998956778266e-10 NA NA NA hsa-miR-29a-3p PHLPP2 -0.699539666833076 1.13935003139957e-13 -0.383642052753594 7.63863404919517e-07 miRNATAP -0.118714509123023 2.23257866105876e-05 NA NA NA hsa-miR-29c-3p PHLPP2 -0.0652732378039769 0.563774115756922 -0.383642052753594 7.63863404919517e-07 miRNATAP -0.106060616909216 7.24119030631034e-06 NA NA NA hsa-miR-542-3p PHLPP2 0.568822103820889 8.5699075257651e-07 -0.383642052753594 7.63863404919517e-07 miRanda -0.123178769302971 7.51908430498907e-08 NA NA NA hsa-miR-182-5p PIK3AP1 2.36408084103818 2.60011457600281e-72 -0.0218481929973899 0.849092455363106 MirTarget -0.182778303086684 1.34564536779175e-11 NA NA NA hsa-miR-30d-3p PIK3AP1 0.264544633563642 0.00755023189129747 -0.0218481929973899 0.849092455363106 miRNATAP -0.112255533575307 0.00485338447007384 NA NA NA hsa-miR-146b-5p PIK3CA 0.631041661167954 6.20366593452965e-08 -0.881957552231161 1.15529457057533e-22 mirMAP -0.10172531442561 0.000163300940659646 NA NA NA hsa-miR-148a-5p PIK3CA 0.572692271754695 2.14306251522796e-06 -0.881957552231161 1.15529457057533e-22 mirMAP -0.111623090383025 1.82115916734019e-05 NA NA NA hsa-miR-200b-3p PIK3CA 2.11744072703209 1.66710572104191e-43 -0.881957552231161 1.15529457057533e-22 TargetScan -0.164394431071602 1.25931440199374e-17 NA NA NA hsa-miR-361-5p PIK3CA -0.0996867547900457 0.095428250126326 -0.881957552231161 1.15529457057533e-22 mirMAP -0.28523073173792 1.02307804662151e-07 NA NA NA hsa-miR-96-5p PIK3CA 3.24596558166606 1.92296159041707e-98 -0.881957552231161 1.15529457057533e-22 TargetScan -0.158039482109614 1.06514304964241e-18 NA NA NA hsa-miR-30b-3p PIK3CD -0.536462824497982 2.41814659272548e-05 -0.316904328019809 0.0048026997053421 MirTarget -0.12656414985523 3.07110294580941e-05 NA NA NA hsa-miR-484 PIK3CD 0.349444060004737 0.00154491249625173 -0.316904328019809 0.0048026997053421 MirTarget;miRNATAP -0.141552727299567 4.96089022907138e-05 NA NA NA hsa-miR-103a-3p PIK3R1 0.610673390034345 1.63113920514646e-12 -1.70627489565512 1.28475917565256e-44 MirTarget;miRNATAP -0.516011898515825 9.39970675304441e-26 NA NA NA hsa-miR-106b-5p PIK3R1 1.22477789913175 2.31787213143326e-32 -1.70627489565512 1.28475917565256e-44 MirTarget;miRNATAP -0.458684807618424 6.41212047089543e-31 NA NA NA hsa-miR-107 PIK3R1 0.678998026350662 2.56224148930538e-16 -1.70627489565512 1.28475917565256e-44 MirTarget;PITA;miRanda;miRNATAP -0.564782368968385 1.29413413077175e-28 NA NA NA hsa-miR-128-3p PIK3R1 0.90296715955238 9.925204823863e-23 -1.70627489565512 1.28475917565256e-44 MirTarget -0.464882086210543 2.18929508374026e-24 25962360 miR 128 3p suppresses hepatocellular carcinoma proliferation by regulating PIK3R1 and is correlated with the prognosis of HCC patients; Mechanistically miR-128-3p was confirmed to regulate PIK3R1 p85α expression thereby suppressing phosphatidylinositol 3-kinase PI3K/AKT pathway activation using qRT-PCR and western blot analysis; Hence we conclude that miR-128-3p which is frequently downregulated in HCC inhibits HCC progression by regulating PIK3R1 and PI3K/AKT activation and is a prognostic marker for HCC patients worse prognosis;progression liver cancer hsa-miR-1301-3p PIK3R1 1.80754972811286 2.96809840886499e-36 -1.70627489565512 1.28475917565256e-44 MirTarget -0.312737279865189 3.51588268231222e-28 NA NA NA hsa-miR-15a-5p PIK3R1 0.812445324157528 4.50263417099822e-20 -1.70627489565512 1.28475917565256e-44 MirTarget -0.467053807879393 1.27196995879228e-22 NA NA NA hsa-miR-15b-5p PIK3R1 0.788659356634696 1.13678211092534e-11 -1.70627489565512 1.28475917565256e-44 MirTarget -0.304923263873542 1.52536411684308e-16 NA NA NA hsa-miR-16-2-3p PIK3R1 0.286355254959005 0.0256946046884474 -1.70627489565512 1.28475917565256e-44 MirTarget -0.187390623272605 5.18756959426033e-08 NA NA NA hsa-miR-16-5p PIK3R1 0.46835991343556 1.91125356547776e-06 -1.70627489565512 1.28475917565256e-44 MirTarget -0.185144677288316 3.4393027748885e-05 NA NA NA hsa-miR-17-5p PIK3R1 0.599531757298529 1.07318660415906e-05 -1.70627489565512 1.28475917565256e-44 MirTarget;TargetScan;miRNATAP -0.305623214527227 2.73465966965389e-22 NA NA NA hsa-miR-182-5p PIK3R1 2.36408084103818 2.60011457600281e-72 -1.70627489565512 1.28475917565256e-44 miRNATAP -0.209986504782115 4.31661758321545e-12 NA NA NA hsa-miR-185-5p PIK3R1 0.482334746589337 3.68475879959415e-09 -1.70627489565512 1.28475917565256e-44 miRNATAP -0.576307293918371 1.92791131730155e-28 NA NA NA hsa-miR-200b-3p PIK3R1 2.11744072703209 1.66710572104191e-43 -1.70627489565512 1.28475917565256e-44 mirMAP -0.193888157381114 6.36921236487692e-13 NA NA NA hsa-miR-200c-3p PIK3R1 2.07038651806183 6.84613386286599e-51 -1.70627489565512 1.28475917565256e-44 mirMAP -0.257145031020416 2.3912254219973e-18 NA NA NA hsa-miR-20a-5p PIK3R1 0.44979911120222 0.000602386964327228 -1.70627489565512 1.28475917565256e-44 MirTarget;miRNATAP -0.329404805146422 7.47770247059783e-24 NA NA NA hsa-miR-21-5p PIK3R1 2.32007501846389 3.08394781855554e-137 -1.70627489565512 1.28475917565256e-44 miRNAWalker2_validate;MirTarget;miRNATAP -0.442813578327247 2.68375696066295e-30 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-29b-3p PIK3R1 0.438847962603729 6.46722433236121e-05 -1.70627489565512 1.28475917565256e-44 MirTarget;miRNATAP -0.212535407562809 1.00431864575306e-07 NA NA NA hsa-miR-320b PIK3R1 0.412787504747938 0.00113457276903985 -1.70627489565512 1.28475917565256e-44 miRNATAP -0.140552558146201 5.57745902628767e-05 NA NA NA hsa-miR-324-3p PIK3R1 0.404877731996915 0.000340036423920834 -1.70627489565512 1.28475917565256e-44 MirTarget;PITA;miRNATAP -0.224592588456891 6.81384539720142e-09 NA NA NA hsa-miR-330-3p PIK3R1 0.432728362037032 0.00041335506960812 -1.70627489565512 1.28475917565256e-44 MirTarget;PITA;miRNATAP -0.144041669892523 7.57903802651215e-05 NA NA NA hsa-miR-361-5p PIK3R1 -0.0996867547900457 0.095428250126326 -1.70627489565512 1.28475917565256e-44 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.278078422988115 0.000207710985727821 NA NA NA hsa-miR-429 PIK3R1 2.84108892259147 3.88520896426515e-64 -1.70627489565512 1.28475917565256e-44 mirMAP;miRNATAP -0.22503499935046 3.34180951947627e-21 NA NA NA hsa-miR-590-3p PIK3R1 1.27438512890514 1.03164723481469e-23 -1.70627489565512 1.28475917565256e-44 miRanda;mirMAP -0.239713681587516 7.2007499793076e-12 NA NA NA hsa-miR-590-5p PIK3R1 0.746515607868274 8.51300479640125e-09 -1.70627489565512 1.28475917565256e-44 MirTarget;PITA;miRanda;miRNATAP -0.209937562895491 3.68537681569131e-10 NA NA NA hsa-miR-629-3p PIK3R1 1.25035919247365 3.91361135469024e-14 -1.70627489565512 1.28475917565256e-44 MirTarget -0.122961236064884 5.54567297669118e-06 NA NA NA hsa-miR-93-5p PIK3R1 1.01788600209314 1.24312921618126e-21 -1.70627489565512 1.28475917565256e-44 MirTarget;miRNATAP -0.499295395694502 3.97678732290002e-38 NA NA NA hsa-miR-96-5p PIK3R1 3.24596558166606 1.92296159041707e-98 -1.70627489565512 1.28475917565256e-44 TargetScan;miRNATAP -0.245653154038236 3.77424010710224e-23 NA NA NA hsa-miR-126-3p PIK3R2 -1.17432628002954 1.02041618253013e-20 1.28293433832686 8.5972816204378e-73 miRNAWalker2_validate;miRTarBase -0.169496363080053 8.52458360518473e-17 21249429;26384552;27578985;26191164;25240815 Endothelial specific intron derived miR 126 is down regulated in human breast cancer and targets both VEGFA and PIK3R2; VEGFA and PIK3R2 were confirmed as the targets of miR-126 by luciferase reporter assay and Western blot;MicroRNA 126 suppresses proliferation of undifferentiated BRAFV600E and BRAFWT thyroid carcinoma through targeting PIK3R2 gene and repressing PI3K AKT proliferation survival signalling pathway; In addition miR-126 was found to act as proliferation suppressor targeting PIK3R2 gene and reducing p85β a regulatory subunit of PI3K kinase protein translation and lower AKT kinase activity;MiR 126 regulates proliferation and invasion in the bladder cancer BLS cell line by targeting the PIK3R2 mediated PI3K/Akt signaling pathway; A direct regulatory relationship between miR-126 and the PIK3R2 gene was demonstrated by luciferase reporter assays; Quantitative real-time polymerase chain reaction was used to measure miR-126 and PIK3R2 expressions; Luciferase assays showed that miR-126 significantly inhibited the PIK3R2 3' untranslated region 3'UTR luciferase reporter activity P<0.05; Overexpression of miR-126 negatively regulated the target gene PIK3R2 and further inhibited the PI3K/Akt signaling pathway thereby inhibiting proliferation migration and invasion and promoting apoptosis in BLS cells;Restoration of miR-126 in EC109 cells induced a reduction in PIK3R2 protein levels accompanied with a substantial reduction in phosphorylated AKT levels in EC109 cells suggesting impairment in PI3K/AKT signaling pathway; The luciferase reporter assay confirmed that PIK3R2 was a direct target of miR-126; Taken together our study suggests that miR-126 functions as a potential tumor suppressor in ESCC progression via regulating PI3K/AKT signaling pathway partly by targeting PIK3R2 and targeting of miR-126 may provide a novel strategy for the diagnosis and treatment of ESCC;MiR 126 3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2; LRP6 and PIK3R2 were identified as targets of miR-126-3p; Silencing LRP6 and PIK3R2 had similar effects of miR-126-3p restoration on metastasis and angiogenesis individually in HCC cells; Furthermore the miR-126-3p level was inversely correlated with LRP6 and PIK3R2 in HCC tissues; In addition the rescue experiments indicated that the metastasis and angiogenesis functions of miR-126-3p were mediated by LRP6 and PIK3R2 ;poor survival;;progression;metastasis breast cancer;thyroid cancer;bladder cancer;esophageal cancer;liver cancer hsa-miR-28-5p PIK3R2 -0.14267073663385 0.0497619246260571 1.28293433832686 8.5972816204378e-73 miRanda -0.170225138245223 4.06181677270291e-06 NA NA NA hsa-miR-30b-5p PIK3R2 -0.46540603860146 2.4709940429591e-05 1.28293433832686 8.5972816204378e-73 miRNATAP -0.111541133552651 3.5447180691879e-06 NA NA NA hsa-miR-30c-5p PIK3R2 -0.393222674580066 8.06961465717306e-05 1.28293433832686 8.5972816204378e-73 miRNAWalker2_validate;miRNATAP -0.102854891876324 0.000118472431445014 NA NA NA hsa-miR-30d-5p PIK3R2 -0.247082219011045 0.00406717930063148 1.28293433832686 8.5972816204378e-73 miRNATAP -0.135541098242265 1.30568664423737e-05 NA NA NA hsa-miR-320a PIK3R2 -0.287921276129346 0.00141693637596294 1.28293433832686 8.5972816204378e-73 miRanda -0.151022063052727 3.16338469232646e-07 NA NA NA hsa-miR-491-5p PIK3R2 -1.04307210952796 1.14586501470398e-15 1.28293433832686 8.5972816204378e-73 miRanda -0.116485616092769 7.44579853361516e-09 NA NA NA hsa-miR-181a-5p PIK3R3 0.635863607630183 6.72486918157744e-11 0.559172960265555 3.98014986716526e-09 MirTarget;miRNATAP -0.146056981056209 1.20649375988888e-05 NA NA NA hsa-miR-19b-3p PIK3R3 0.139124391010031 0.255658553872934 0.559172960265555 3.98014986716526e-09 MirTarget;miRNATAP -0.109224067759979 4.71454242795867e-05 NA NA NA hsa-miR-217 PIK3R3 -0.616728851115349 8.64977119332241e-05 0.559172960265555 3.98014986716526e-09 PITA;miRanda -0.118432783728797 1.05730160913028e-08 NA NA NA hsa-miR-25-3p PIK3R3 -0.00546790775985073 0.944061771389468 0.559172960265555 3.98014986716526e-09 MirTarget -0.129911102729152 0.00226643466666436 NA NA NA hsa-miR-26a-5p PIK3R3 -0.481180084102235 1.82368246174758e-10 0.559172960265555 3.98014986716526e-09 miRNATAP -0.28216506702381 5.37056936747542e-11 NA NA NA hsa-miR-374a-5p PIK3R3 -0.239709888246804 0.00309819667153627 0.559172960265555 3.98014986716526e-09 mirMAP -0.121442827230575 0.0029020400924295 NA NA NA hsa-miR-374b-5p PIK3R3 -0.466781429946153 1.17238311619116e-08 0.559172960265555 3.98014986716526e-09 mirMAP -0.120196538768652 0.00271578207935794 NA NA NA hsa-miR-381-3p PIK3R3 -1.28028930293369 1.61402949866141e-23 0.559172960265555 3.98014986716526e-09 mirMAP -0.140767078074606 1.38941193259491e-08 NA NA NA hsa-miR-532-5p PIK3R3 0.0386976222860582 0.682018574136515 0.559172960265555 3.98014986716526e-09 mirMAP -0.140279487612118 5.85354025061255e-05 NA NA NA hsa-miR-92a-3p PIK3R3 -0.342420481092807 0.000732275969501111 0.559172960265555 3.98014986716526e-09 MirTarget;miRNATAP -0.227757876133252 1.19233380886192e-12 NA NA NA hsa-miR-125a-5p PIK3R5 -0.635694700253268 3.6703235721638e-11 0.311128035887536 0.0038375952038782 MirTarget;miRanda -0.280593880513026 8.91996620676494e-14 NA NA NA hsa-miR-182-5p PIK3R5 2.36408084103818 2.60011457600281e-72 0.311128035887536 0.0038375952038782 MirTarget -0.108305079676846 2.32094065834864e-05 NA NA NA hsa-miR-149-5p PIK3R6 0.218233101703391 0.261629158371175 0.849989357566209 2.34485680256543e-14 MirTarget -0.101631195527119 3.02500849596628e-07 NA NA NA hsa-miR-106b-5p PKN2 1.22477789913175 2.31787213143326e-32 -0.63639482831944 1.50179769438523e-18 MirTarget;miRNATAP -0.114239154757245 1.178885174372e-06 NA NA NA hsa-miR-20a-5p PKN2 0.44979911120222 0.000602386964327228 -0.63639482831944 1.50179769438523e-18 MirTarget;miRNATAP -0.100093891540082 1.81419998508007e-07 NA NA NA hsa-miR-542-3p PKN2 0.568822103820889 8.5699075257651e-07 -0.63639482831944 1.50179769438523e-18 miRanda -0.16710086389033 7.72196298664446e-15 NA NA NA hsa-miR-589-5p PKN2 0.367182541239722 1.67804815542873e-05 -0.63639482831944 1.50179769438523e-18 MirTarget -0.133451275589961 6.75986465512432e-06 NA NA NA hsa-miR-93-5p PKN2 1.01788600209314 1.24312921618126e-21 -0.63639482831944 1.50179769438523e-18 MirTarget;miRNATAP -0.136735009972103 3.03854470600213e-09 NA NA NA hsa-miR-193b-3p PKN3 -0.0550374855074773 0.65382485898551 -0.750170791167584 4.61888022870859e-14 miRNAWalker2_validate -0.104941205578436 0.000202089128637252 NA NA NA hsa-miR-324-3p PKN3 0.404877731996915 0.000340036423920834 -0.750170791167584 4.61888022870859e-14 miRNAWalker2_validate -0.240315613310727 1.52079016229133e-15 NA NA NA hsa-miR-615-3p PKN3 0.659240580933614 0.000360214386152507 -0.750170791167584 4.61888022870859e-14 miRNAWalker2_validate -0.123169002961014 2.74274371498086e-11 NA NA NA hsa-miR-17-3p PPP2CA 0.721439431208839 7.95900548819742e-10 0.355964216272682 1.3164574185057e-12 MirTarget -0.133330530645377 1.5958296816568e-20 NA NA NA hsa-miR-425-5p PPP2CB 0.719898949241718 6.01079610190374e-08 -0.508468028837195 3.29413468049735e-15 miRNAWalker2_validate -0.1434305833926 3.027811361313e-18 NA NA NA hsa-miR-590-3p PPP2CB 1.27438512890514 1.03164723481469e-23 -0.508468028837195 3.29413468049735e-15 miRanda -0.10511834935696 4.0251015210593e-09 NA NA NA hsa-miR-128-3p PPP2R1B 0.90296715955238 9.925204823863e-23 -1.24069690315388 3.45729939213988e-47 MirTarget -0.109583188561297 0.000963818534703709 NA NA NA hsa-miR-146b-5p PPP2R1B 0.631041661167954 6.20366593452965e-08 -1.24069690315388 3.45729939213988e-47 miRanda -0.141184532696467 1.00140046078863e-07 NA NA NA hsa-miR-15a-5p PPP2R1B 0.812445324157528 4.50263417099822e-20 -1.24069690315388 3.45729939213988e-47 MirTarget -0.195736754254626 1.30304715741401e-08 NA NA NA hsa-miR-200b-3p PPP2R1B 2.11744072703209 1.66710572104191e-43 -1.24069690315388 3.45729939213988e-47 MirTarget -0.190114683335312 5.20726875191601e-24 NA NA NA hsa-miR-200c-3p PPP2R1B 2.07038651806183 6.84613386286599e-51 -1.24069690315388 3.45729939213988e-47 MirTarget -0.226923946553815 2.68646974148231e-28 21248297 Western blotting showed that knockdown of miR-200c expression was associated with increased expression of PPP2R1B a subunit of protein phosphatase 2A which resulted in reduced expression of phospho-Akt esophageal cancer hsa-miR-30d-3p PPP2R1B 0.264544633563642 0.00755023189129747 -1.24069690315388 3.45729939213988e-47 mirMAP -0.157184793756038 6.17261426855798e-07 NA NA NA hsa-miR-429 PPP2R1B 2.84108892259147 3.88520896426515e-64 -1.24069690315388 3.45729939213988e-47 MirTarget;miRanda;miRNATAP -0.148415458438853 1.78268855237664e-18 NA NA NA hsa-let-7i-5p PPP2R2A -0.333419326258177 3.56960810786801e-08 -0.167330560503955 0.00931403270504818 miRNATAP -0.107110666603503 0.00353562731274897 NA NA NA hsa-miR-106b-5p PPP2R2A 1.22477789913175 2.31787213143326e-32 -0.167330560503955 0.00931403270504818 MirTarget;miRNATAP -0.11513710270418 1.67830991715771e-08 NA NA NA hsa-miR-374a-5p PPP2R2A -0.239709888246804 0.00309819667153627 -0.167330560503955 0.00931403270504818 mirMAP -0.108346705337341 7.22514521796377e-05 NA NA NA hsa-miR-107 PPP2R2B 0.678998026350662 2.56224148930538e-16 -1.93953123840583 5.78989293907757e-30 miRanda -0.382818751276931 1.02239949522717e-07 NA NA NA hsa-miR-141-3p PPP2R2B 2.99777540204168 1.26370498493024e-78 -1.93953123840583 5.78989293907757e-30 TargetScan -0.315881073340513 5.36482191004776e-21 NA NA NA hsa-miR-7-1-3p PPP2R2B 0.686579567507582 1.20731554448806e-07 -1.93953123840583 5.78989293907757e-30 mirMAP -0.161881570908929 0.000489281404028886 NA NA NA hsa-miR-181a-2-3p PPP2R2C -0.0718643470098321 0.422077954889554 2.25419279038223 3.53879659770262e-10 MirTarget -0.44823553635027 0.00136468286381967 NA NA NA hsa-miR-218-5p PPP2R2C -1.02621788318234 2.9221925445771e-23 2.25419279038223 3.53879659770262e-10 miRNATAP -0.43489406545735 0.000214380392578878 NA NA NA hsa-miR-374b-5p PPP2R2C -0.466781429946153 1.17238311619116e-08 2.25419279038223 3.53879659770262e-10 miRNATAP -0.466306325492385 0.00215151258507057 NA NA NA hsa-miR-500a-3p PPP2R2C 0.139523673241448 0.185528510741158 2.25419279038223 3.53879659770262e-10 MirTarget -0.359889661556384 0.00241165449343688 NA NA NA hsa-miR-106b-5p PPP2R3A 1.22477789913175 2.31787213143326e-32 -0.626967779883103 1.20861407864987e-09 mirMAP -0.111862284465381 0.000767196160499777 NA NA NA hsa-miR-128-3p PPP2R3A 0.90296715955238 9.925204823863e-23 -0.626967779883103 1.20861407864987e-09 MirTarget -0.108493816479353 0.00424285824211482 NA NA NA hsa-miR-181a-5p PPP2R3A 0.635863607630183 6.72486918157744e-11 -0.626967779883103 1.20861407864987e-09 MirTarget -0.181048478657749 5.66732314331517e-07 NA NA NA hsa-miR-181b-5p PPP2R3A 1.22749257795094 1.21998198830919e-27 -0.626967779883103 1.20861407864987e-09 MirTarget -0.14052218325066 4.35547864865999e-06 NA NA NA hsa-miR-181c-5p PPP2R3A 0.574847839298605 4.51287502066478e-07 -0.626967779883103 1.20861407864987e-09 MirTarget -0.12347379024146 7.3120008996013e-05 NA NA NA hsa-miR-215-5p PPP2R3B -1.35643065897947 2.3180023440257e-20 0.568314513936472 2.22868668753805e-08 miRNAWalker2_validate -0.119533240278851 2.90833201921148e-07 NA NA NA hsa-miR-107 PPP2R5A 0.678998026350662 2.56224148930538e-16 -0.271185658300753 0.000577998233135009 miRanda -0.116676163127242 0.000291888356596273 NA NA NA hsa-miR-455-5p PPP2R5A 0.284641224662773 0.0357572339594277 -0.271185658300753 0.000577998233135009 miRanda -0.148237717752069 5.19586125294051e-14 NA NA NA hsa-miR-132-3p PPP2R5E -0.0365213889238607 0.668598301088667 0.018935638230313 0.793647310855766 MirTarget -0.117209424280952 5.86934317627525e-05 NA NA NA hsa-miR-146b-5p PPP2R5E 0.631041661167954 6.20366593452965e-08 0.018935638230313 0.793647310855766 miRanda -0.108519433912314 2.38654321669596e-07 NA NA NA hsa-miR-181a-5p PPP2R5E 0.635863607630183 6.72486918157744e-11 0.018935638230313 0.793647310855766 MirTarget;mirMAP -0.172705968640064 4.32215395940648e-12 NA NA NA hsa-miR-181b-5p PPP2R5E 1.22749257795094 1.21998198830919e-27 0.018935638230313 0.793647310855766 MirTarget;mirMAP -0.122508311764402 6.82476700676191e-09 NA NA NA hsa-miR-181c-5p PPP2R5E 0.574847839298605 4.51287502066478e-07 0.018935638230313 0.793647310855766 MirTarget;mirMAP -0.122046895343518 1.35193024529625e-08 NA NA NA hsa-miR-361-5p PPP2R5E -0.0996867547900457 0.095428250126326 0.018935638230313 0.793647310855766 miRanda -0.362844376404808 1.47810752731031e-18 NA NA NA hsa-miR-374a-3p PPP2R5E 0.34122393953022 2.28714105382856e-05 0.018935638230313 0.793647310855766 MirTarget -0.108150800102799 0.000444740858970142 NA NA NA hsa-miR-374a-5p PPP2R5E -0.239709888246804 0.00309819667153627 0.018935638230313 0.793647310855766 mirMAP -0.175200740577251 9.34615884892216e-09 NA NA NA hsa-miR-381-3p PPP2R5E -1.28028930293369 1.61402949866141e-23 0.018935638230313 0.793647310855766 MirTarget;mirMAP -0.104778657482713 2.09043362809501e-08 NA NA NA hsa-miR-455-5p PPP2R5E 0.284641224662773 0.0357572339594277 0.018935638230313 0.793647310855766 miRanda -0.1463471269634 3.89164487655405e-16 NA NA NA hsa-miR-542-3p PPP2R5E 0.568822103820889 8.5699075257651e-07 0.018935638230313 0.793647310855766 miRanda -0.14687324447004 3.28141828349973e-12 NA NA NA hsa-miR-128-3p PRKAA1 0.90296715955238 9.925204823863e-23 -0.889722997110138 6.68547349344403e-22 miRNAWalker2_validate -0.203711118503965 2.87586951912717e-09 NA NA NA hsa-miR-130b-3p PRKAA1 1.38122163823846 1.00932179737836e-26 -0.889722997110138 6.68547349344403e-22 miRNAWalker2_validate;MirTarget;miRNATAP -0.102341099053503 2.95589264304139e-05 NA NA NA hsa-miR-148b-3p PRKAA1 1.0552576751879 1.6679285705231e-29 -0.889722997110138 6.68547349344403e-22 miRNAWalker2_validate;MirTarget -0.144460761230633 1.70583649615035e-05 NA NA NA hsa-miR-15a-5p PRKAA1 0.812445324157528 4.50263417099822e-20 -0.889722997110138 6.68547349344403e-22 MirTarget -0.317615312742036 2.43574896376608e-19 NA NA NA hsa-miR-15b-5p PRKAA1 0.788659356634696 1.13678211092534e-11 -0.889722997110138 6.68547349344403e-22 MirTarget -0.113980224735419 3.72256160222342e-05 NA NA NA hsa-miR-182-5p PRKAA1 2.36408084103818 2.60011457600281e-72 -0.889722997110138 6.68547349344403e-22 MirTarget -0.12957259010959 7.74120097344211e-09 NA NA NA hsa-miR-20a-5p PRKAA1 0.44979911120222 0.000602386964327228 -0.889722997110138 6.68547349344403e-22 mirMAP -0.127482024088913 2.29558821504195e-07 NA NA NA hsa-miR-301a-3p PRKAA1 1.32170168375632 2.12280368957514e-15 -0.889722997110138 6.68547349344403e-22 MirTarget;miRNATAP -0.111641895127187 5.76713491206456e-09 NA NA NA hsa-miR-30d-3p PRKAA1 0.264544633563642 0.00755023189129747 -0.889722997110138 6.68547349344403e-22 MirTarget;miRNATAP -0.150165899451655 4.84642713120332e-06 NA NA NA hsa-miR-429 PRKAA1 2.84108892259147 3.88520896426515e-64 -0.889722997110138 6.68547349344403e-22 miRNATAP -0.141667891084818 1.10952877434855e-15 NA NA NA hsa-miR-454-3p PRKAA1 1.28177672191095 6.84024979484587e-21 -0.889722997110138 6.68547349344403e-22 MirTarget;miRNATAP -0.134963384764347 6.03158027315945e-09 NA NA NA hsa-miR-769-5p PRKAA1 0.564871766265874 8.16695101656941e-10 -0.889722997110138 6.68547349344403e-22 MirTarget;PITA -0.156145941793697 8.51973967414274e-06 NA NA NA hsa-let-7d-5p PRKAA2 0.410958462607207 6.07808182756787e-06 -0.579847549865762 0.0120328308442779 MirTarget -0.253927364326718 0.00361978152126917 NA NA NA hsa-let-7i-5p PRKAA2 -0.333419326258177 3.56960810786801e-08 -0.579847549865762 0.0120328308442779 MirTarget -0.434247047501726 0.00099975084098266 NA NA NA hsa-miR-106b-5p PRKAA2 1.22477789913175 2.31787213143326e-32 -0.579847549865762 0.0120328308442779 mirMAP -0.407309465215687 2.87950289122621e-08 NA NA NA hsa-miR-130b-3p PRKAA2 1.38122163823846 1.00932179737836e-26 -0.579847549865762 0.0120328308442779 MirTarget -0.236959907465252 7.269812745952e-05 NA NA NA hsa-miR-142-5p PRKAA2 1.16509303143593 1.96641162209073e-13 -0.579847549865762 0.0120328308442779 mirMAP -0.308494315080149 2.59550351577519e-10 NA NA NA hsa-miR-146a-5p PRKAA2 0.102405752365098 0.486975378688068 -0.579847549865762 0.0120328308442779 MirTarget -0.303068137585273 1.45529396860407e-08 NA NA NA hsa-miR-146b-5p PRKAA2 0.631041661167954 6.20366593452965e-08 -0.579847549865762 0.0120328308442779 MirTarget -0.466570898192425 2.87992191143047e-12 NA NA NA hsa-miR-15b-3p PRKAA2 1.12898579025388 1.18050912917317e-14 -0.579847549865762 0.0120328308442779 mirMAP -0.245586956351096 3.75844225220248e-06 NA NA NA hsa-miR-16-2-3p PRKAA2 0.286355254959005 0.0256946046884474 -0.579847549865762 0.0120328308442779 mirMAP -0.268112091248467 1.5313383960563e-05 NA NA NA hsa-miR-17-5p PRKAA2 0.599531757298529 1.07318660415906e-05 -0.579847549865762 0.0120328308442779 mirMAP -0.294736369867022 3.19418199550823e-07 NA NA NA hsa-miR-181a-5p PRKAA2 0.635863607630183 6.72486918157744e-11 -0.579847549865762 0.0120328308442779 mirMAP -0.425977225966366 1.12553795472933e-07 NA NA NA hsa-miR-181b-5p PRKAA2 1.22749257795094 1.21998198830919e-27 -0.579847549865762 0.0120328308442779 mirMAP -0.402894448830963 2.57710767596897e-09 NA NA NA hsa-miR-19a-3p PRKAA2 0.817507952171848 3.76285090469625e-08 -0.579847549865762 0.0120328308442779 mirMAP -0.223371384798626 2.3136263934908e-05 NA NA NA hsa-miR-203a-3p PRKAA2 1.67084394806148 6.06522273352132e-14 -0.579847549865762 0.0120328308442779 MirTarget -0.127404255594052 0.000267649490057364 NA NA NA hsa-miR-20a-5p PRKAA2 0.44979911120222 0.000602386964327228 -0.579847549865762 0.0120328308442779 mirMAP -0.346885432705756 7.06226699554703e-09 NA NA NA hsa-miR-21-3p PRKAA2 2.17476065946878 5.15299442583591e-75 -0.579847549865762 0.0120328308442779 mirMAP -0.257680881978514 2.16873974422669e-05 NA NA NA hsa-miR-22-5p PRKAA2 0.238328801322329 0.0377496760295305 -0.579847549865762 0.0120328308442779 mirMAP -0.236371300737833 0.000631744857978216 NA NA NA hsa-miR-301a-3p PRKAA2 1.32170168375632 2.12280368957514e-15 -0.579847549865762 0.0120328308442779 MirTarget -0.199811074896772 2.06431978910307e-05 NA NA NA hsa-miR-30b-5p PRKAA2 -0.46540603860146 2.4709940429591e-05 -0.579847549865762 0.0120328308442779 MirTarget -0.239154411813247 0.000844867804972869 NA NA NA hsa-miR-30d-3p PRKAA2 0.264544633563642 0.00755023189129747 -0.579847549865762 0.0120328308442779 MirTarget;miRNATAP -0.292199265560775 0.000269897209542169 NA NA NA hsa-miR-30d-5p PRKAA2 -0.247082219011045 0.00406717930063148 -0.579847549865762 0.0120328308442779 MirTarget -0.309661319614685 0.000819102278720473 NA NA NA hsa-miR-335-3p PRKAA2 -1.88448538773649 2.09538234398335e-32 -0.579847549865762 0.0120328308442779 MirTarget -0.125289555996901 0.00892382059630128 NA NA NA hsa-miR-340-5p PRKAA2 1.0857159627596 4.94471062546756e-24 -0.579847549865762 0.0120328308442779 MirTarget -0.322802145820831 6.75061943901237e-06 NA NA NA hsa-miR-500a-5p PRKAA2 0.209805801129289 0.119177819862398 -0.579847549865762 0.0120328308442779 mirMAP -0.411392653460274 3.23149194968278e-12 NA NA NA hsa-miR-589-5p PRKAA2 0.367182541239722 1.67804815542873e-05 -0.579847549865762 0.0120328308442779 MirTarget -0.416057776223634 7.30350634476401e-06 NA NA NA hsa-miR-590-3p PRKAA2 1.27438512890514 1.03164723481469e-23 -0.579847549865762 0.0120328308442779 mirMAP;miRNATAP -0.252553744754276 6.76594678507493e-05 NA NA NA hsa-miR-93-5p PRKAA2 1.01788600209314 1.24312921618126e-21 -0.579847549865762 0.0120328308442779 mirMAP -0.403745175357699 2.28259007015548e-08 NA NA NA hsa-miR-98-5p PRKAA2 0.656336869298973 9.03113188590466e-15 -0.579847549865762 0.0120328308442779 miRNAWalker2_validate;MirTarget -0.543058840822084 3.50897487370215e-09 NA NA NA hsa-miR-181b-5p PRKCA 1.22749257795094 1.21998198830919e-27 -1.70723657875247 1.7688165305473e-34 mirMAP -0.11742885805708 0.0062191009662321 NA NA NA hsa-miR-181c-5p PRKCA 0.574847839298605 4.51287502066478e-07 -1.70723657875247 1.7688165305473e-34 mirMAP -0.151937361609795 0.00048559417186175 NA NA NA hsa-miR-183-5p PRKCA 2.95681814393872 1.09965777344285e-92 -1.70723657875247 1.7688165305473e-34 miRNATAP -0.382401275003301 4.39521361355191e-39 NA NA NA hsa-miR-193b-3p PRKCA -0.0550374855074773 0.65382485898551 -1.70723657875247 1.7688165305473e-34 miRNAWalker2_validate -0.158591304154604 9.61914010087921e-05 NA NA NA hsa-miR-194-5p PRKCA 0.467781870340114 1.0386810618625e-07 -1.70723657875247 1.7688165305473e-34 mirMAP -0.194734764385864 0.000579018161354159 NA NA NA hsa-miR-200b-3p PRKCA 2.11744072703209 1.66710572104191e-43 -1.70723657875247 1.7688165305473e-34 TargetScan -0.338107140907763 7.43601898887161e-30 NA NA NA hsa-miR-200c-3p PRKCA 2.07038651806183 6.84613386286599e-51 -1.70723657875247 1.7688165305473e-34 miRNATAP -0.384868851727468 4.79014422420278e-32 NA NA NA hsa-miR-29b-2-5p PRKCA -0.263499971180298 0.0400001755611965 -1.70723657875247 1.7688165305473e-34 mirMAP -0.365220324605892 6.60788568306249e-22 NA NA NA hsa-miR-30d-3p PRKCA 0.264544633563642 0.00755023189129747 -1.70723657875247 1.7688165305473e-34 mirMAP -0.185045137833304 0.000246654172160224 NA NA NA hsa-miR-339-5p PRKCA -0.211548290465418 0.0685885445822389 -1.70723657875247 1.7688165305473e-34 mirMAP -0.116903398908303 0.00661066095178211 NA NA NA hsa-miR-340-5p PRKCA 1.0857159627596 4.94471062546756e-24 -1.70723657875247 1.7688165305473e-34 mirMAP -0.157880969288124 0.000482930053726641 NA NA NA hsa-miR-375 PRKCA 2.09893673049766 1.68351780396203e-16 -1.70723657875247 1.7688165305473e-34 miRNAWalker2_validate -0.240131861837491 2.29799527522168e-39 NA NA NA hsa-miR-429 PRKCA 2.84108892259147 3.88520896426515e-64 -1.70723657875247 1.7688165305473e-34 miRanda;miRNATAP -0.302006017947741 7.14515269636082e-30 NA NA NA hsa-let-7d-5p PRLR 0.410958462607207 6.07808182756787e-06 1.27351378761725 5.27607801731441e-13 miRNATAP -0.272028887463407 5.47002790868371e-05 NA NA NA hsa-let-7g-5p PRLR 0.0200025784917734 0.791635298815256 1.27351378761725 5.27607801731441e-13 miRNATAP -0.233689111950248 0.00432433307799952 NA NA NA hsa-let-7i-5p PRLR -0.333419326258177 3.56960810786801e-08 1.27351378761725 5.27607801731441e-13 miRNATAP -0.891894607115349 5.32674075990505e-19 NA NA NA hsa-miR-130a-3p PRLR -1.15024914519431 6.51290317952496e-19 1.27351378761725 5.27607801731441e-13 mirMAP -0.279947802725982 1.17999346885074e-09 NA NA NA hsa-miR-130b-3p PRLR 1.38122163823846 1.00932179737836e-26 1.27351378761725 5.27607801731441e-13 mirMAP -0.209027118831004 6.01635346843064e-06 NA NA NA hsa-miR-144-5p PRLR -2.75914786146772 1.55794225315972e-50 1.27351378761725 5.27607801731441e-13 mirMAP -0.236248227916033 1.47242221155932e-14 NA NA NA hsa-miR-146a-5p PRLR 0.102405752365098 0.486975378688068 1.27351378761725 5.27607801731441e-13 mirMAP -0.216643212140334 1.7523725363952e-07 NA NA NA hsa-miR-146b-5p PRLR 0.631041661167954 6.20366593452965e-08 1.27351378761725 5.27607801731441e-13 mirMAP -0.273438844577925 1.499175856621e-07 NA NA NA hsa-miR-148a-5p PRLR 0.572692271754695 2.14306251522796e-06 1.27351378761725 5.27607801731441e-13 mirMAP -0.134817074373083 0.00772994956929194 NA NA NA hsa-miR-15b-3p PRLR 1.12898579025388 1.18050912917317e-14 1.27351378761725 5.27607801731441e-13 mirMAP -0.20538661765261 5.74164111841047e-07 NA NA NA hsa-miR-186-5p PRLR -0.426987301460247 1.04129380549315e-05 1.27351378761725 5.27607801731441e-13 mirMAP -0.401617770723117 1.53804520349439e-10 NA NA NA hsa-miR-19b-1-5p PRLR 0.647704962003062 3.07687667945597e-07 1.27351378761725 5.27607801731441e-13 mirMAP -0.240190669375244 5.7283975939384e-07 NA NA NA hsa-miR-20a-3p PRLR -0.218902047543766 0.179868363625547 1.27351378761725 5.27607801731441e-13 mirMAP -0.100128257767749 0.00798148046637221 NA NA NA hsa-miR-218-5p PRLR -1.02621788318234 2.9221925445771e-23 1.27351378761725 5.27607801731441e-13 miRNATAP -0.29105637420739 4.53057925873605e-07 NA NA NA hsa-miR-22-5p PRLR 0.238328801322329 0.0377496760295305 1.27351378761725 5.27607801731441e-13 mirMAP -0.384645099058839 3.6032680629659e-13 NA NA NA hsa-miR-26a-5p PRLR -0.481180084102235 1.82368246174758e-10 1.27351378761725 5.27607801731441e-13 mirMAP -0.305382365474339 0.000163973613234094 NA NA NA hsa-miR-29a-5p PRLR 0.334183773952055 0.00445137265870197 1.27351378761725 5.27607801731441e-13 mirMAP -0.148313104785018 0.00465886457453621 NA NA NA hsa-miR-320a PRLR -0.287921276129346 0.00141693637596294 1.27351378761725 5.27607801731441e-13 miRanda -0.273303361157844 6.0014526817036e-05 NA NA NA hsa-miR-320b PRLR 0.412787504747938 0.00113457276903985 1.27351378761725 5.27607801731441e-13 miRanda -0.159127665629827 0.00105334402901543 NA NA NA hsa-miR-340-5p PRLR 1.0857159627596 4.94471062546756e-24 1.27351378761725 5.27607801731441e-13 mirMAP -0.195410559131314 0.000446695295062688 NA NA NA hsa-miR-362-5p PRLR -0.248676340901797 0.0726089851456282 1.27351378761725 5.27607801731441e-13 mirMAP -0.415662010540809 7.35379555005751e-22 NA NA NA hsa-miR-374a-5p PRLR -0.239709888246804 0.00309819667153627 1.27351378761725 5.27607801731441e-13 mirMAP -0.390711651873163 2.52402677048321e-07 NA NA NA hsa-miR-374b-5p PRLR -0.466781429946153 1.17238311619116e-08 1.27351378761725 5.27607801731441e-13 mirMAP -0.442449213962816 2.60603911325557e-09 NA NA NA hsa-miR-376c-3p PRLR -1.23221625820254 6.08631316990405e-17 1.27351378761725 5.27607801731441e-13 mirMAP -0.151370891986081 0.000240338414209719 NA NA NA hsa-miR-381-3p PRLR -1.28028930293369 1.61402949866141e-23 1.27351378761725 5.27607801731441e-13 mirMAP -0.173226031819158 0.00020060668215085 NA NA NA hsa-miR-455-5p PRLR 0.284641224662773 0.0357572339594277 1.27351378761725 5.27607801731441e-13 miRanda -0.409759103505849 2.29594134369905e-20 NA NA NA hsa-miR-485-3p PRLR -0.866822427729628 4.5194665263492e-11 1.27351378761725 5.27607801731441e-13 mirMAP -0.136788990260707 0.00333895603024827 NA NA NA hsa-miR-495-3p PRLR -1.67685437612894 1.70634429769052e-34 1.27351378761725 5.27607801731441e-13 mirMAP -0.167341960291121 0.000100878102927204 NA NA NA hsa-miR-576-5p PRLR 0.0649387707567879 0.588791966096287 1.27351378761725 5.27607801731441e-13 mirMAP -0.236764414410884 3.49177562421495e-06 NA NA NA hsa-miR-582-5p PRLR 0.201134299470983 0.149482495292227 1.27351378761725 5.27607801731441e-13 PITA -0.210704711037092 2.00132240557531e-06 NA NA NA hsa-miR-589-3p PRLR 0.0263457630961427 0.866577983997705 1.27351378761725 5.27607801731441e-13 mirMAP -0.125275909775997 0.00176650789337072 NA NA NA hsa-miR-9-5p PRLR 0.734772328078424 0.00261982843636383 1.27351378761725 5.27607801731441e-13 miRNATAP -0.111874928161752 7.39049424501855e-06 NA NA NA hsa-miR-103a-3p PTEN 0.610673390034345 1.63113920514646e-12 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase -0.310265672952247 8.75438711531989e-26 26511107;24828205 LncRNA GAS5 induces PTEN expression through inhibiting miR 103 in endometrial cancer cells; To investigate the expression of GAS5 PTEN and miR-103 RT-PCR was performed; Finally we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells; Through inhibiting the expression of miR-103 GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis and thus could be an important mediator in the pathogenesis of endometrial cancer;Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN ; endometrial cancer;colorectal cancer hsa-miR-106b-5p PTEN 1.22477789913175 2.31787213143326e-32 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.275865014410931 5.66481319436072e-31 26722252;24842611;26238857 Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells;MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance ;cell migration;drug resistance breast cancer;gastric cancer;colorectal cancer hsa-miR-107 PTEN 0.678998026350662 2.56224148930538e-16 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;PITA;miRanda -0.294379341856167 1.4825572602859e-21 NA NA NA hsa-miR-130b-3p PTEN 1.38122163823846 1.00932179737836e-26 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.10664357535475 8.50670598014416e-08 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-132-3p PTEN -0.0365213889238607 0.668598301088667 -0.737945156702629 1.26474514880987e-22 miRNATAP -0.136713479453887 1.19438197426319e-05 NA NA NA hsa-miR-141-3p PTEN 2.99777540204168 1.26370498493024e-78 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.12717832397844 6.47904977288109e-18 27644195;24742567 Involvement of microRNA 141 3p in 5 fluorouracil and oxaliplatin chemo resistance in esophageal cancer cells via regulation of PTEN; Western blot exhibited altered protein levels of PTEN Akt and PI3k with miR-141-3p inhibitor; An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples; Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo;PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis drug resistance;tumorigenesis esophageal cancer;endometrial cancer hsa-miR-142-5p PTEN 1.16509303143593 1.96641162209073e-13 -0.737945156702629 1.26474514880987e-22 MirTarget;PITA -0.109721832085825 1.70710191686431e-11 NA NA NA hsa-miR-146b-5p PTEN 0.631041661167954 6.20366593452965e-08 -0.737945156702629 1.26474514880987e-22 miRanda -0.188813122906075 1.83574416282237e-17 NA NA NA hsa-miR-16-1-3p PTEN 1.30142784811196 2.53343713753744e-29 -0.737945156702629 1.26474514880987e-22 MirTarget -0.13766907248918 3.37650605787999e-09 NA NA NA hsa-miR-17-5p PTEN 0.599531757298529 1.07318660415906e-05 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.152588360384734 1.28038204158757e-15 26629823;27400681;23391506;23133552;24462867;26318586;26215320;25634356;26500892;24912422;23418359 We hypothesized that knocking down the oncogenic microRNA oncomiR miR-17-5p might restore the expression levels of PDCD4 and PTEN tumor suppressor proteins illustrating a route to oligonucleotide therapy of TNBC; Contrary to conventional wisdom antisense knockdown of oncomiR miR-17-5p guide strand reduced PDCD4 and PTEN proteins by 1.8±0.3 fold in human TNBC cells instead of raising them; Bioinformatics analysis and folding energy calculations revealed that mRNA targets of miR-17-5p guide strand such as PDCD4 and PTEN could also be regulated by miR-17-3p passenger strand;GFRα2 prompts cell growth and chemoresistance through down regulating tumor suppressor gene PTEN via Mir 17 5p in pancreatic cancer; Mechanically we discovered that high GFRα2 expression level leads to PTEN inactivation via enhancing Mir-17-5p level;We found that these phenotypes were the results of miR-17 targeting PTEN;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;miR 17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression; Expression of miR-17 was negatively correlated with PTEN in OS tissues;Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR 17 family in prostate cancer; Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo;In addition ERβ expression significantly increased in calycosin-treated HCT-116 cells followed by a decrease of miR-17 and up-regulation of PTEN; Our results indicate that calycosin has an inhibitory effect on CRC which might be obtained by ERβ-mediated regulation of miR-17 and PTEN expression;The High Expression of the microRNA 17 92 Cluster and its Paralogs and the Downregulation of the Target Gene PTEN Is Associated with Primary Cutaneous B Cell Lymphoma Progression;MicroRNA 17 5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling; miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells;MicroRNA 17 5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression; We found that PTEN was a target of miR-17-5p in the colon cancer cells and their context-specific interactions were responsible for multiple drug-resistance; Chemotherapy was found to increase the expression levels of miR-17-5p which further repressed PTEN levels contributing to the development of chemo-resistance; MiR-17-5p is a predictive factor for chemotherapy response and a prognostic factor for overall survival in CRC which is due to its regulation of PTEN expression;The mature miR-17-5p exerted this function by repressing the expression of PTEN ;drug resistance;;;metastasis;;;progression;drug resistance;metastasis;drug resistance;poor survival; breast cancer;pancreatic cancer;glioblastoma;sarcoma;sarcoma;prostate cancer;colorectal cancer;B cell lymphoma;ovarian cancer;colorectal cancer;liver cancer hsa-miR-181a-5p PTEN 0.635863607630183 6.72486918157744e-11 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.217453583427793 2.70335682235321e-16 24532253;27534652;24685694;27264420;26323677;26750720 LPS induced miR 181a promotes pancreatic cancer cell migration via targeting PTEN and MAP2K4; Furthermore we predicted and confirmed that both PTEN and MAP2K4 were miR-181a targets; Pancreatic cancer tissues analysis showed that PTEN and MAP2K4 were all negatively correlated with miR-181a;From our results down-regulation of miR-181a increased the expression of PTEN and decreased phosphorylation of Akt and FOXO1;miR-181a performs this function by inhibiting the expression of PTEN leading to an increase of phosphorylated AKT which triggers metabolic shift;The expression of PTEN was regulated by IL-1β-stimulated miR-181a expression; In a PTEN reporter plasmid miR-181a binding site mutations were introduced; Furthermore a siRNA strategy was used to find that IL-1B regulates miR-181a expression via NF-κB and then regulates PTEN expression; Consequently repression of PTEN by miR-181a promotes colon cancer cell proliferation;Luciferase assays were performed to assess the ability of miR-181a to target the PTEN promoter and regulation of PTEN expression by miR-181a was assessed by western blot analysis and RT-PCR; PTEN was identified as a direct target of miR-181a; Our findings demonstrate that miR-181a expression in lung adenocarcinoma is associated with EMT progression potentially through targeting of PTEN;The expression of miR-181A and PTEN in CRC patients with late liver metastases was higher than that of the normal control group whereas the expression of miR-181A and PTEN was lower in all pathological groups TNM I-TNM IV; The expression of miR-181A and PTEN in the colon cell line NCM460 was lower than that of the colon cancer SW620 cell line; Upregulation of miR-181A promoted cell viability and inhibited apoptosis of SW620 cells suppressed PTEN expression and activated phosphorylation of AKT p-AKT in SW620 cells; Additionally downregulation of miR-181A inhibited cell viability of SW620 cells through promotion of PTEN and inhibition of p-AKT cell migration;;;;progression;metastasis pancreatic cancer;cervical and endocervical cancer;colon cancer;colorectal cancer;lung cancer;colorectal cancer hsa-miR-181b-5p PTEN 1.22749257795094 1.21998198830919e-27 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;MirTarget;miRNATAP -0.223803397924892 6.72795816887185e-24 NA NA NA hsa-miR-181c-5p PTEN 0.574847839298605 4.51287502066478e-07 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.128349050965034 2.50139620016373e-08 25695913 miR 181c promotes proliferation via suppressing PTEN expression in inflammatory breast cancer; In this study we showed that miR-181c as an oncogene promoted proliferation and it inhibited PTEN protein expression by targeting 3'-UTR of PTEN mRNA in IBC SUM149 cells; Thus targeting miR-181c and restoration of PTEN can be used in conjunction with other therapies to prevent progression of IBC progression breast cancer hsa-miR-181d-5p PTEN 0.782618466877349 1.2821116358212e-09 -0.737945156702629 1.26474514880987e-22 MirTarget -0.120384368723786 2.66954476137279e-09 27006767 Moreover hsa-miR-181d was upregulated to control expression a tumor suppressor gene PTEN to protect the cancer cell from apoptosis endometrial cancer hsa-miR-18a-5p PTEN 0.969066793715868 1.69069796425892e-08 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase -0.135863155421297 1.28041670903141e-19 24681249;27291152 However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis;miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis metastasis; colon cancer;esophageal cancer hsa-miR-19a-3p PTEN 0.817507952171848 3.76285090469625e-08 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.132652171590163 2.97639287894663e-14 27445062;24831732;21853360;26098000;24681249;27289489;25107371 The target genes of miR-19a such as ABCA1 and PTEN that had been suppressed by miR recovered their expression through CAP treatment;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN;Transfection of miR-19a and -19b mimics reversed the up-regulations of IGF2R and PTEN gene expression and abrogated the GSE induced anti-proliferative response;The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid; Meanwhile gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN; The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN ;;drug resistance;;metastasis;drug resistance; breast cancer;breast cancer;breast cancer;lung cancer;colon cancer;lung cancer;bladder cancer hsa-miR-19b-3p PTEN 0.139124391010031 0.255658553872934 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.108487626225012 5.17000304535224e-07 24831732;21853360;26098000;24681249 Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN ;drug resistance;;metastasis breast cancer;breast cancer;lung cancer;colon cancer hsa-miR-200a-3p PTEN 2.55218480823434 2.96135446816238e-51 -0.737945156702629 1.26474514880987e-22 miRNATAP -0.109411985169924 5.36101217094024e-14 22637745;24742567;21408027 Re expression of miR 200 by novel approaches regulates the expression of PTEN and MT1 MMP in pancreatic cancer; We initially compared the expression profile of miR-200 family PTEN and MT1-MMP expression in six pancreatic cancer PC cell lines by qRT-PCR and western blot analysis; We found loss of expression of miR-200a b and c in chemo-resistant PC cell lines which was correlated with loss of PTEN and over-expression of MT1-MMP; The expression of miR-200 family and PTEN was significantly re-expressed whereas the expression of MT1-MMP was down-regulated by CDF and BR-DIM treatment; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment;PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants ;tumorigenesis; pancreatic cancer;endometrial cancer;pancreatic cancer hsa-miR-200b-3p PTEN 2.11744072703209 1.66710572104191e-43 -0.737945156702629 1.26474514880987e-22 TargetScan;mirMAP -0.130827329200595 5.08867014435072e-16 22637745;21408027 Re expression of miR 200 by novel approaches regulates the expression of PTEN and MT1 MMP in pancreatic cancer; We initially compared the expression profile of miR-200 family PTEN and MT1-MMP expression in six pancreatic cancer PC cell lines by qRT-PCR and western blot analysis; The expression of miR-200 family and PTEN was significantly re-expressed whereas the expression of MT1-MMP was down-regulated by CDF and BR-DIM treatment; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants ; pancreatic cancer;pancreatic cancer hsa-miR-200c-3p PTEN 2.07038651806183 6.84613386286599e-51 -0.737945156702629 1.26474514880987e-22 mirMAP;miRNATAP -0.143190372668334 7.03511808200308e-16 24682933;22637745 In conclusion miR-200c functions as an oncogene in colon cancer cells through regulating tumor cell apoptosis survival invasion and metastasis as well as xenograft tumor growth through inhibition of PTEN expression and p53 phosphorylation;Forced over-expression or silencing of miR-200c followed by either CDF or BR-DIM treatment of MIAPaCa-2 cells altered the morphology of cells wound-healing capacity colony formation and the expression of MT1-MMP and PTEN; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment metastasis;poor survival; colon cancer;pancreatic cancer hsa-miR-20a-5p PTEN 0.44979911120222 0.000602386964327228 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.21141055042385 3.32787653231297e-27 26031366 The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction; MiR-20a levels were increased in HCC cell lines and tissues whereas PTEN was inversely correlated with it; PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance drug resistance liver cancer hsa-miR-21-5p PTEN 2.32007501846389 3.08394781855554e-137 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;mirMAP -0.241962926381789 5.21884080644212e-25 26387181;21471222;26905520;25027758;21820606;24930006;22547075;26666820;23951172;25647415;22678116;22832383;19212625;22267008;26384051;22322462;27644439;21468550;20113523;21806946;24154840;27611950;24780321;23036707;23684551;26559642;26731559;26847601;27350731;24331411;25909227;24293118;26289851;24324076;23201752;17681183;25963606;27188433;22958183;22956424;19730150;21842656;21104017;23894315;23548551;26236156;24460329;20092645;25973032;25563770;24659669;26230405;25543482;20048743;27725205;23466500;22922228;25058005;20223231;27220494;24763002;24221338;22120473;21408027;23174819;22978663;25799148;26741162;23226804;26787105;26864640;26311740;25603978;26975392 In addition treated with 5-AZA resulted in significant increases of miR-21 expression in both MCF-7 and MDA-MB-231 cells P < 0.01 with the protein level of PTEN increased in MCF-7 cell which was further involved in the downregulation of AKT;Here we demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474 SKBR3 and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody whereas protein expression of the PTEN gene a miR-21 target was reduced; Rescuing PTEN expression with a p3XFLAG-PTEN-mut construct with deleted miR-21 targeting sequence at its 3' UTR restored the growth inhibition of trastuzumab in the resistant cells by inducing PTEN activation and AKT inhibition; In vivo administering miR-21 antisense oligonucleotides restored trastuzumab sensitivity in the resistant breast cancer xenografts by inducing PTEN expression whereas injection of miR-21 mimics conferred trastuzumab resistant in the sensitive breast tumors via PTEN silence;The effect of ZA on miR-21 expression was quantified by qRT-PCR and the amount of PTEN protein and its targets were analyzed by Western blot;In the same patients we also compared miR-21 expression with the expression of its presumed target PTEN; miR-21 expression levels were found to significantly correlate with tumour size r = 0.403 p = 0.009; Spearman's rank whereas no relation was found between miR-21 and PTEN expression levels Kruskal-Wallis test;MicroRNA 21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN; TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-21 and tumor suppressor gene PTEN protein; We showed that upregulation of miR-21 in MCF-7/ADR cells was concurrent with downregulation of PTEN protein; Overexpression of PTEN could mimic the same effects of miR-21 inhibitor in MCF-7/ADR cells and PTEN-siRNA could increase the resistance of MCF-7 cells to ADR; MiR-21 inhibitor could increase PTEN protein expression and the luciferase activity of a PTEN 3' untranslated region-based reporter construct in MCF-7/ADR cells; Dysregulation of miR-21 plays critical roles in the ADR resistance of breast cancer at least in part via targeting PTEN;In present study we determined the miR-21 levels in TNBC specimens and TNBC cell levels in vitro and then identified the role of miR-21 on tumor cell proliferation apoptosis and then identified PTEN as the possible target of the microRNA;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;PTEN a direct target gene of miR-21 was significantly downregulated in gemcitabine-resistant breast cancer cells and restoration of PTEN expression blocked miR-21-induced EMT and gemcitabine resistance;The results showed that knockdown of miR-21 by antagomir-21 decreased cell proliferation and induced apoptosis via targeting PTEN both in 4T1 cells and HUVECs;MiR-21 upregulation contributes to PTEN downregulation which is beneficial for the activation of PI3K/AKT signaling;Furthermore miR-21-induced upregulation of CSF-1 mRNA and its transcription were prevented by expression of PTEN mRNA lacking 3'-untranslated region UTR and miR-21 recognition sequence; Our results reveal a novel mechanism for the therapeutic function of fish oil diet that blocks miR-21 thereby increasing PTEN levels to prevent expression of CSF-1 in breast cancer;After miR-21 was transfected in MCF-7 cells PTEN protein level was measured by Western blot; Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt resulting in accumulation of Bad p21/WAF1/CIP1 and p27/KIP1;To further determine the potential involvement of miR-21 in breast cancer we have evaluated the expression level of miR-21 by stem-loop real-time RT-PCR based on SYBR-Green I in human invasive ductal carcinoma of the breast and we have correlated the results with clinicopathologic features and PTEN protein expression; The expression levels of miR-21 were correlated with PTEN and commonly used clinicopathologic features of breast cancer; Expression of miR-21 was negatively correlated with expression of PTEN P=0.013; These findings suggest that PTEN is possibly one of the targets of miR-21 in breast cancer and high expression of mir-21 indicates a more aggressive phenotype;microRNA 21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN; Thus in this study we focused on the expression and significance of miR-21 in gastric cancer tissues and the role of miR-21 in the biological behaviour and the expression of PTEN in gastric cancer cells; Western blotting and the Luciferase Reporter Assay were used to evaluate the change of PTEN expression after lowered expression of miR-21 in gastric cancer cell lines; The western blot results and Luciferase Reporter Assay demonstrated that PTEN expression was remarkably increased after miR-21 inhibition P<0.05 microRNA-21 expression was upregulated in gastric carcinoma tissues and was significantly associated with the degree of differentiation of tumour tissues local invasion and lymph node metastasis;Furthermore we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog PTEN the result of which can be rescued by miR-21 inhibitor;Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon;Bmi-1 also regulates p53 and PTEN via miR-21;The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting respectively in tumor tissues as well as adjacent non-tumor mucosa; miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues; A negative correlation between expression of miR-21 and PTEN was established in vivo;MicroRNA 21 inhibitor sensitizes human glioblastoma cells U251 PTEN mutant and LN229 PTEN wild type to taxol; Human glioblastoma U251 PTEN-mutant and LN229 PTEN wild-type cells were treated with taxol and the miR-21 inhibitor in a poly amidoamine PAMAM dendrimer alone or in combination; Interestingly the above data suggested that in both the PTEN mutant and the wild-type GBM cells miR-21 blockage increased the chemosensitivity to taxol; Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;NTR1 activation stimulates expression of miR-21 and miR-155 in colonocytes via Akt and NF-κB to down-regulate PTEN and SOCS1 and promote growth of tumors in mice;Correlations between the expression levels of miR-21 PTEN and p-AKT were analyzed by real-time PCR and Western blot test in HER2-positive GC cell lines; Overexpression of miR-21 down-regulated PTEN expression increased AKT phosphorylation and did not affect HER2 expression; Inversely suppression of miR-21 increased PTEN expression and down-regulated AKT phosphorylation but still did not affect HER2 expression;MicroRNA 21 promotes TGF β1 induced epithelial mesenchymal transition in gastric cancer through up regulating PTEN expression; In GC tissues the expressions of miR-21 Akt and p-Akt were up-regulated while PTEN expression was down-regulated; These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression;Expressions of PTEN were significantly down-regulated in CRC tissues and negatively correlated with expressions of Notch-1 r2=0.5207 p<0.01 and miR-21 r2=0.6996 p<0.01; These data indicate that the crosstalk between Notch-1 and miR-21 is involved in CRC development through degradation of PTEN;Over-expression of miR-21 suppressed its target PTEN and disrupted acinar morphogenesis;MicroRNA 21 suppresses PTEN and hSulf 1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways;There was overexpression of the miR-21 target genes PTEN by 67% and caspase-3 by 15% upon cotreatment;We previously reported that microRNA-21 miR-21 was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target PTEN; Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN stem-loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status; miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression; These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus;Triptolide reduces proliferation and enhances apoptosis of human non small cell lung cancer cells through PTEN by targeting miR 21; To the best of our knowledge the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21;Quantitative real-time PCR qRT-PCR and Western blot were used to detect the expression levels of miR-21 and PTEN in HCT116 HT29 Colo32 and SW480 CRC cell lines; Also the expression levels of PTEN mRNA and its downstream proteins AKT and PI3K in HCT116 cells after downregulating miR-21 were investigated; In comparing the levels of PTEN protein and downstream AKT and PI3K in HCT116 cells after downregulation of miR-21 expression the levels of AKT and PI3K protein expression significantly decreased P < 0.05; PTEN is one of the direct target genes of miR-21;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;MiR-21 level was inversely correlated with the levels of FOXO1 and PTEN in DLBCL cell lines; MiR-21 also down-regulated PTEN expression and consequently activated the PI3K/AKT/mTOR pathway which further decreased FOXO1 expression;MiR 21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non small cell lung cancer A549 cells; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA was performed to modulate the expression of microRNA-21 and PTEN under the treatment of curcumin; Moreover the protein level of PTEN a putative target of microRNA-21 was significantly elevated in curcumin-treated A549 cells as determined by Western blot analysis; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA significantly P < 0.05 reversed the growth suppression and apoptosis induction by curcumin compared to corresponding controls; Our data suggest a novel molecular mechanism in which inhibition of microRNA-21 and upregulation of PTEN mediate the anticancer activities of curcumin in NSCLC cells;PTEN gene expression was performed as a known target of miR-21;We also found that Rawq01 up-regulated the expression of PTEN through mir-21 inhibition and therefore inhibited the PI3K-AKT pathway;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;MicroRNA 21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer; PTEN was shown to be a direct target of miR-21 and to contribute to miR-21 effects on cell invasion; Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9 both downstream mediators of PTEN involved in cell migration and invasion; Aberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth migration and invasion;Overexpression of miR 21 promotes the proliferation and migration of cervical cancer cells via the inhibition of PTEN; The aim of this study was to examine the expression of miR-21 and PTEN in cervical cancer specimens using quantitative PCR; miR-21 was upregulated in the cervical cancer specimens negatively correlating with the PTEN mRNA level; Transfection of the miR-21 mimics was markedly promoted whereas the miR-21 inhibitor suppressed the proliferation migration and invasion of cervical cancer cells with a significant inhibition of PTEN expression; The present study showed the upregulation of miR-21 in invasive cervical cancers and confirmed the promotion of miR-21 with regard to the proliferation migration and invasion in cervical cancer cells via inhibiting the PTEN expression;The expressions of miR-21 PTEN PI3K and AKT were detected in 89 esophageal cancer samples and 58 adjacent normal tissues respectively; MiR-21 PI3K and AKT have higher expressions but PTEN has lower expression in esophageal cancer tissues compared with adjacent normal tissues; Further PTEN was a target gene of miR-21;The expression level of miR-21 and PTEN messenger RNA were measured by quantitative real-time reverse transcription polymerase chain reaction or reverse transcription polymerase chain reaction; miR-21 was overexpressed and PTEN was suppressed in established radioresistant TE-R60 cells compared with the parent cells 1.3-fold and 70.83%; The inhibition of miR-21 significantly increased the cells' radiosensitivity P < 0.05 and the PTEN protein expression 2.3-fold in TE-1 cells; Knockdown of PTEN in anti-miR-21 TE-1 cells could abrogate the miR-21 inhibition-induced radiosensitization P < 0.05; Inhibition of miR-21 increased radiosensitivity of esophageal cancer TE-1 cells and this effect was possibly through the activation of PTEN;MicroRNA 21 miR 21 expression promotes growth metastasis and chemo or radioresistance in non small cell lung cancer cells by targeting PTEN; Taken together these results provide evidence to show the promotion role of miR-21 in NSCLC development through modulation of the PTEN signaling pathway;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Role of microRNA 21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma; To evaluate the role of miR-21 and PTEN cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells; In Eca109 when transfected with miR-21 mimics accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40% which resulted in the promotion of cell proliferation; However when transfected with miR-21 inhibitor expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79% which resulted in the suppression of cell proliferation; Furthermore there was a significantly inverse correlation between miR-21 expression and PTEN protein levels p < 0.05; The author concluded that MiR-21 was overexpressed in vitro and ESCC and promoted the cell proliferation might target PTEN at post-transcriptional level and regulated the cancer invasion in Kazakh's ESCC;Difluorinated curcumin CDF restores PTEN expression in colon cancer cells by down regulating miR 21; Indeed our current data demonstrate a marked downregulation of PTEN in SCID mice xenografts of miR-21 over-expressing colon cancer HCT116 cells; Colonospheres that are highly enriched in cancer stem/stem like cells reveal increased miR-21 expression and decreased PTEN; Difluorinated curcumin CDF a novel analog of the dietary ingredient curcumin which has been shown to inhibit the growth of 5-Flurouracil + Oxaliplatin resistant colon cancer cells downregulated miR-21 in chemo-resistant colon cancer HCT116 and HT-29 cells and restored PTEN levels with subsequent reduction in Akt phosphorylation;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells;Finally invasion and metastasis assays were performed and alteration in mir-21 PTEN AKT and pAKT level was evaluated in these cells; Enhanced invasion and metastasis increased miR-21 expression decreased PTEN elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells;In the present study we investigated the role of miR-21 and its potential as a therapeutic target in two prostate cancer cell lines characterized by different miR-21 expression levels and PTEN gene status;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;Expressions of microRNA-21 miR-21 PTEN MMP9 and p47 were detected by qPCR;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;microRNA 21 Regulates Cell Proliferation and Migration and Cross Talk with PTEN and p53 in Bladder Cancer; MicroRNA-21 regulates proliferation and migration of bladder cancer cells and cross talk with PTEN and p53 in bladder cancer;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status; To explore whether miR-21 can serve as a therapeutic target for glioblastoma we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 PTEN mutant and LN229 PTEN wild-type GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo; Taken together our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated; In conclusion our data suggest that mUA can suppress cell viability in DU145 cells through modulating miR-21 and its downstream series-wound targets including PTEN Akt and Wnt/β-catenin signaling;miR 21 confers cisplatin resistance in gastric cancer cells by regulating PTEN; In addition miR-21 induced cell survival and cisplatin resistance through downregulating the expression of phosphatase and tension homolog deleted on chromosome 10 PTEN and activation of Akt pathway;Finally we demonstrate that modulation of tumor suppressors PTEN and p53 in U87 cells does not affect the decrease in miR-21 levels associated with PDGF-B overexpression;This study aimed to investigate whether NSCLC miR-21 mediated resistance to TKIs also results from Pten targeting; Here we show miR-21 promotes cancer by negatively regulating Pten expression in human NSCLC tissues: high miR-21 expression levels were associated with shorter DFS in 47 NSCLC patients; high miR-21/low Pten expression levels indicated a poor TKI clinical response and shorter overall survival in another 46 NSCLC patients undergoing TKI treatment; In vitro assays showed that miR-21 was up-regulated concomitantly to down-regulation of Pten in pc-9/GR cells in comparison with pc-9 cells; Moreover over-expression of miR-21 significantly decreased gefitinib sensitivity by down-regulating Pten expression and activating Akt and ERK pathways in pc-9 cells while miR-21 knockdown dramatically restored gefitinib sensitivity of pc-9/GR cells by up-regulation of Pten expression and inactivation of AKT and ERK pathways in vivo and in vitro;MicroRNA 21 miR 21 represses tumor suppressor PTEN and promotes growth and invasion in non small cell lung cancer NSCLC; We identified the role of miR-21 in non-small cell lung cancer NSCLC and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation; Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot respectively; Tumor tissues showed an inverse correlation between miR-21 and PTEN protein; miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines; miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC;MiR-21 overexpression decreases PTEN increases p-Akt and subsequently increases HIF-1α expression while miR-21 inhibition results in increased PTEN decreased p-Akt and then decreased HIF-1α;This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma DLBCL paraffin-embedded tissues and evaluate its potential relevance with clinical characteristics; In patients with DLBCL the expression level of miR-21 was negatively correlated with the level of PTEN protein; These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL;Down regulation of PTEN expression modulated by dysregulated miR 21 contributes to the progression of esophageal cancer; We demonstrated that knockdown of miR-21 significantly increased expression of PTEN protein; Our findings suggest that miR-21 could be a potential oncomiR probably by regulation of PTEN and a novel prognostic factor for ESCC patients;The levels of mir-21 did not associate with the expression of PTEN an important tumour suppressor in CRC and one of many putative targets of miR-21 but interestingly was associated with stage of disease in the PTEN expressing tumours;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants;The PTEN protein levels in CRC tissues and cells had an inverse correlation with miR-21 expression; MiR-21 targets PTEN at the post-transcriptional level and regulates cell proliferation and invasion in CRC;These findings demonstrate a novel role of AR in the regulation of miR-21 and its target PTEN in growth factor-induced colon cancer cell growth;Whether miR-21 regulated PTEN expression was assessed by luciferase assay; The RNA and protein levels of PTEN were significantly decreased by exogenous miR-21 and the 3'-untranslated region of PTEN was shown to be a target of miR-21;In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX;microRNA 21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer; We performed a qRT-PCR assay with miR-21 and PTEN in 16 paired EEC tumor tissues and adjacent non-tumor endometrium; To validate the putative binding site of miR-21 in the 3' untranslated region 3'-UTR of PTEN messenger RNA mRNA a dual-luciferase reporter assay was carried out; The upregulation of miR-21 led to a significant decrease in the PTEN protein expression level P=0.007; The downregulation of miR-21 led to a significant increase in PTEN protein P=0.002; In conclusion we demonstrated that the expression of PTEN protein but not mRNA was negatively directly regulated by miR-21 in the KLE cell line; The overexpression of miR-21 modulated EEC cell proliferation through the downregulation of PTEN;The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR 21 and promoter methylation; In this study we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC; PTEN mRNA level was negatively correlated with miR-21 level r = -0.595 P < 0.001; PTEN expression was also correlated directly with the PTEN mRNA level r = 0.583 P < 0.001 and conversely with miR-21 level r = -0.632 P < 0.001; This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;Exposure of HCC cells to sorafenib led to an increase in miR-21 expression a decrease in PTEN expression and sequential Akt activation;MicroRNA 21 controls hTERT via PTEN in human colorectal cancer cell proliferation; The aim of this study was to determine a role of microRNA-21 miRNA-21 in colorectal cancer CRC and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue PTEN;Relevance of miR 21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells; We identified the tumor suppressor gene PTEN as a target of miR-21 and determined the mechanism of its regulation throughout reporter construct plasmids; Using this model we analyzed the expression of miR-21 and PTEN as well as functional effects such as autophagy and apoptosis induction; In SiHa cells there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells; We conclude that miR-21 post-transcriptionally down-regulates the expression of PTEN to promote cell proliferation and cervical cancer cell survival ;drug resistance;;;drug resistance;;;drug resistance;;;;;;metastasis;differentiation;;tumorigenesis;;;;;;;;;progression;;;;;drug resistance;;;;;;cell migration;;;;metastasis;drug resistance;;;;;;;metastasis;;;;;;;progression;malignant trasformation;;drug resistance;poor survival;;drug resistance;poor survival;;;;progression;staging;;;;;;;;drug resistance;;;poor survival breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;gastric cancer;cervical and endocervical cancer;colon cancer;gastric cancer;bladder cancer;glioblastoma;colon cancer;gastric cancer;gastric cancer;colorectal cancer;lung cancer;liver cancer;glioblastoma;melanoma;lung squamous cell cancer;colorectal cancer;lung squamous cell cancer;B cell lymphoma;lung squamous cell cancer;thyroid cancer;esophageal cancer;glioblastoma;liver cancer;cervical and endocervical cancer;esophageal cancer;esophageal cancer;lung squamous cell cancer;pancreatic cancer;lung cancer;esophageal cancer;colon cancer;B cell lymphoma;colon cancer;pancreatic cancer;prostate cancer;liver cancer;lung cancer;gastric cancer;bladder cancer;B cell lymphoma;glioblastoma;prostate cancer;gastric cancer;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;cervical and endocervical cancer;B cell lymphoma;esophageal cancer;colorectal cancer;pancreatic cancer;colorectal cancer;colon cancer;lung squamous cell cancer;lung cancer;endometrial cancer;colorectal cancer;pancreatic cancer;liver cancer;colorectal cancer;cervical and endocervical cancer hsa-miR-25-3p PTEN -0.00546790775985073 0.944061771389468 -0.737945156702629 1.26474514880987e-22 miRTarBase;MirTarget;miRNATAP -0.2154254166509 2.47655742416089e-10 NA NA NA hsa-miR-30d-3p PTEN 0.264544633563642 0.00755023189129747 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.168386426148969 2.57365382931766e-10 NA NA NA hsa-miR-30d-5p PTEN -0.247082219011045 0.00406717930063148 -0.737945156702629 1.26474514880987e-22 mirMAP -0.119749089269445 0.000108226912832517 NA NA NA hsa-miR-30e-5p PTEN 0.293491764699475 0.000372291967271834 -0.737945156702629 1.26474514880987e-22 mirMAP -0.102649949652368 0.00147806085442833 NA NA NA hsa-miR-32-5p PTEN 1.38676603945192 2.29428299685976e-34 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.17719636259429 1.05244002548292e-15 24123284;25647261;23617834 In this study we determined the levels of the correlation between and the clinical significance of the expression of miR-32 and phosphatase and tensin homologue PTEN a tumor suppressor targeted by miR-32 in CRC; The levels of miR-32 and PTEN gene expression in 35 colorectal carcinoma samples 35 corresponding cancer-adjacent tissue samples 27 colorectal adenoma samples and 16 normal tissue samples were quantified using real-time quantitative reverse transcriptase-polymerase chain reaction; The relationship between the miR-32 and PTEN protein expression and clinicopathological factors was analyzed; Significant upregulation of miR-32 expression and reduction of PTEN were identified in CRC tissues; An inverse relationship between miR-32 and PTEN protein expression was identified; MiR-32 and PTEN expression were inversely correlated and miR-32 may be associated with the development of CRC;MiR 32 induces cell proliferation migration and invasion in hepatocellular carcinoma by targeting PTEN; Besides miRNA-32 down-regulates PTEN through binding to 3'-UTR of PTEN mRNA from luciferase reporter assay and the expression level of miR-32 could affect the proliferation migration and invasion of liver cancer cell lines via PTEN/Akt signaling pathway; Down-expression of PTEN could significantly attenuate the inhibitory effects of knockdown miR-32 on the proliferation migration and invasion of liver cancer cells suggesting that miR-32 could be a potential target for HCC treatment;MicroRNA 32 miR 32 regulates phosphatase and tensin homologue PTEN expression and promotes growth migration and invasion in colorectal carcinoma cells; In this study we identified the potential effects of miR-32 on some important biological properties of CRC cells and clarified the regulation of PTEN by miR-32; The 3'-untranslated region 3'-UTR of PTEN combined with miR-32 was verified by dual-luciferase reporter assay; Gain-of-function and loss-of-function studies showed that overexpression of miR-32 promoted SW480 cell proliferation migration and invasion reduced apoptosis and resulted in downregulation of PTEN at a posttranscriptional level; However miR-32 knock-down inhibited these processes in HCT-116 cells and enhanced the expression of PTEN protein; In addition we further identified PTEN as the functional downstream target of miR-32 by directly targeting the 3'-UTR of PTEN; Our results demonstrated that miR-32 was involved in tumorigenesis of CRC at least in part by suppression of PTEN ;;tumorigenesis colorectal cancer;liver cancer;colorectal cancer hsa-miR-340-5p PTEN 1.0857159627596 4.94471062546756e-24 -0.737945156702629 1.26474514880987e-22 miRNATAP -0.219336420279721 1.16026230714932e-20 NA NA NA hsa-miR-374a-5p PTEN -0.239709888246804 0.00309819667153627 -0.737945156702629 1.26474514880987e-22 MirTarget;mirMAP -0.10880061073271 0.000942835734329093 NA NA NA hsa-miR-429 PTEN 2.84108892259147 3.88520896426515e-64 -0.737945156702629 1.26474514880987e-22 PITA;miRanda;mirMAP;miRNATAP -0.13365636792818 1.00274002812788e-20 NA NA NA hsa-miR-455-5p PTEN 0.284641224662773 0.0357572339594277 -0.737945156702629 1.26474514880987e-22 miRanda -0.18068645365542 3.51124729527242e-21 NA NA NA hsa-miR-532-5p PTEN 0.0386976222860582 0.682018574136515 -0.737945156702629 1.26474514880987e-22 PITA;mirMAP;miRNATAP -0.207204663361429 1.01792292857881e-13 NA NA NA hsa-miR-542-3p PTEN 0.568822103820889 8.5699075257651e-07 -0.737945156702629 1.26474514880987e-22 PITA;miRanda;miRNATAP -0.120703250512885 1.08830015884254e-07 NA NA NA hsa-miR-590-3p PTEN 1.27438512890514 1.03164723481469e-23 -0.737945156702629 1.26474514880987e-22 MirTarget;PITA;miRanda;mirMAP -0.130863097442091 5.26569206971815e-10 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting; miR-590-3p was found to activate PI3K-AKT signaling pathway by down-regulating PTEN to promote AKT1-S473 phosphorylation liver cancer hsa-miR-590-5p PTEN 0.746515607868274 8.51300479640125e-09 -0.737945156702629 1.26474514880987e-22 mirMAP -0.110007008517724 5.05737696379977e-08 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-92a-3p PTEN -0.342420481092807 0.000732275969501111 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.102588930556491 8.69033110484314e-05 26432332;25515201;24137349;23546593;23133552;24026406 Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor; Taken together these findings suggested that miR-92a could promote growth metastasis and chemoresistance in NSCLC cells at least partially by targeting PTEN;MiR 92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN Mediated PI3K/AKT Pathway; The expression of miR-92a PTEN and E-cadherin was analyzed by real-time PCR; In addition there was a negative correlation between levels of miR-92a and the PTEN gene p < 0.0001; The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;The expression levels of miR-92a and phosphatase and tensin homologue PTEN were detected by qRT-PCR and western blot; In addition the regulation of PTEN by miR-92a was evaluated by qRT-PCR western blot and luciferase reporter assays; There was an inverse correlation between the levels of miR-92a and PTEN in CRC tissues; The overexpression of miR-92a in CRC cells decreased PTEN expression at the translational level and decreased PTEN-driven luciferase-reporter activity; Our results demonstrated that miR-92a induced EMT and regulated cell growth migration and invasion in the SW480 cells at least partially via suppression of PTEN expression metastasis;drug resistance;metastasis;cell migration;;worse prognosis;; lung squamous cell cancer;colorectal cancer;cervical and endocervical cancer;liver cancer;sarcoma;colorectal cancer hsa-miR-93-5p PTEN 1.01788600209314 1.24312921618126e-21 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.264691004436426 2.0924597171148e-29 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-let-7c-5p PTK2 -1.9415540533505 9.55250745007793e-48 0.34551403513174 7.59893035573032e-07 miRNAWalker2_validate -0.145523582147357 2.05630494198177e-18 NA NA NA hsa-miR-139-5p PTK2 -3.25846663863766 2.62018989415003e-112 0.34551403513174 7.59893035573032e-07 miRanda -0.109205234931847 1.69268288863156e-14 NA NA NA hsa-miR-199a-5p PTK2 -0.314703593105081 0.00909304983051124 0.34551403513174 7.59893035573032e-07 miRanda -0.117208700092394 3.25513954280803e-09 NA NA NA hsa-miR-199b-5p PTK2 -1.37022396448377 3.25758057539223e-26 0.34551403513174 7.59893035573032e-07 miRanda -0.147263766750288 9.5558677434372e-17 NA NA NA hsa-miR-379-5p PTK2 -1.29872802686271 5.33844249801446e-22 0.34551403513174 7.59893035573032e-07 MirTarget -0.128620655965672 6.92581265300639e-14 26944318 Moreover miR-379-5p exerted this function by directly targeting focal adhesion kinase FAK 3'-UTR and repressing FAK expression thus leading to suppression of AKT signaling; Furthermore the tumor suppressive effects of miR-379-5p in HCC cells were reversed by activating AKT signaling or restoring FAK expression; In clinical samples of HCC miR-379-5p negatively correlated with FAK which was up-regulated in HCC liver cancer hsa-miR-101-3p RAC1 -0.126035286271986 0.145997832808138 0.176628662773421 0.000153658997491136 miRNAWalker2_validate;MirTarget -0.139972978942608 1.93650351223228e-14 25202416;24649082;25057058 In previous studies the role of miRNA-101 miR-101 in tumors has been identified as a tumor suppressor and until now the role of miR-101 and Rac1 in thyroid cancer has remained undefined; Rac1 was demonstrated to be negatively regulated by miR-101 at the post-transcriptional level via a specific target site within the 3' untranslated region by dual-luciferase reporter assay; The expression of Rac1 was also observed to inversely correlate with miR-101 expression in PTC tissues; knockdown of Rac1 by shRNA inhibited thyroid cancer cell migration and invasion resembling that of miR-101 overexpression; Thus these findings suggested that miR-101 acts as a novel suppressor by targeting the Rac1 gene and inhibiting thyroid cancer cell migration and invasion;miR 101 inhibits cell proliferation by targeting Rac1 in papillary thyroid carcinoma; In the present study we investigated the role of microRNA-101 miR-101 in PTC via targeting of Ras-related C3 botulinum toxin substrate 1 Rac1; Moreover algorithm-based and experimental strategies verified Rac1 as a direct target of miR-101 in the K1 cell line; Taken together these findings suggest that miR-101 inhibited PTC growth via the downregulation of Rac1 expression providing a better understanding of miRNA-modulated signaling networks for future cancer therapeutics;Mir-101 inhibitors significantly increased the luciferase activities of RAC1 3'UTR; Overexpression of mir-101 increased the expression of RAC1 while inhibition of mir-101 suppressed RAC1 expression; mir-101 can suppress RAC1 gene expression by targeting the specific sequence of RAC1 3'UTR cell migration;; thyroid cancer;thyroid cancer;colorectal cancer hsa-miR-106b-5p RBL2 1.22477789913175 2.31787213143326e-32 -0.777587758921412 2.2971094499013e-22 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.263898121665047 3.64871109439286e-25 NA NA NA hsa-miR-107 RBL2 0.678998026350662 2.56224148930538e-16 -0.777587758921412 2.2971094499013e-22 miRanda -0.214816939727489 9.7538516274009e-11 NA NA NA hsa-miR-17-5p RBL2 0.599531757298529 1.07318660415906e-05 -0.777587758921412 2.2971094499013e-22 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.11529529846799 1.64112965057067e-08 NA NA NA hsa-miR-20a-5p RBL2 0.44979911120222 0.000602386964327228 -0.777587758921412 2.2971094499013e-22 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.182553356518434 3.46682203211793e-18 NA NA NA hsa-miR-429 RBL2 2.84108892259147 3.88520896426515e-64 -0.777587758921412 2.2971094499013e-22 miRanda -0.114178875200587 9.05972696116893e-14 NA NA NA hsa-miR-93-5p RBL2 1.01788600209314 1.24312921618126e-21 -0.777587758921412 2.2971094499013e-22 MirTarget;miRNATAP -0.257781038097889 8.64400419240358e-25 NA NA NA hsa-miR-107 RELN 0.678998026350662 2.56224148930538e-16 -4.67087443281158 1.50848083381937e-55 miRanda -0.883231456020123 7.63489077408637e-12 NA NA NA hsa-miR-128-3p RELN 0.90296715955238 9.925204823863e-23 -4.67087443281158 1.50848083381937e-55 miRNAWalker2_validate;miRTarBase;MirTarget -0.862415996814179 5.48710419496746e-14 NA NA NA hsa-miR-142-3p RELN 1.53951237846104 2.06769936282034e-17 -4.67087443281158 1.50848083381937e-55 miRanda -0.178494228225566 0.00252091909305814 NA NA NA hsa-miR-15a-5p RELN 0.812445324157528 4.50263417099822e-20 -4.67087443281158 1.50848083381937e-55 MirTarget;miRNATAP -0.962503350000195 7.5057160857068e-16 NA NA NA hsa-miR-15b-5p RELN 0.788659356634696 1.13678211092534e-11 -4.67087443281158 1.50848083381937e-55 MirTarget;miRNATAP -0.703940415046345 1.79893150960565e-14 NA NA NA hsa-miR-16-5p RELN 0.46835991343556 1.91125356547776e-06 -4.67087443281158 1.50848083381937e-55 MirTarget;miRNATAP -0.793690699181889 4.62244726075035e-13 NA NA NA hsa-miR-200b-3p RELN 2.11744072703209 1.66710572104191e-43 -4.67087443281158 1.50848083381937e-55 MirTarget;TargetScan -0.657437580196194 1.99168662140682e-23 NA NA NA hsa-miR-200c-3p RELN 2.07038651806183 6.84613386286599e-51 -4.67087443281158 1.50848083381937e-55 MirTarget -0.830139052765864 6.0952837560973e-31 NA NA NA hsa-miR-27a-3p RELN 0.388496361379095 1.42373532086331e-05 -4.67087443281158 1.50848083381937e-55 MirTarget;miRNATAP -0.574127490254185 2.47928818802126e-06 NA NA NA hsa-miR-331-5p RELN 0.682437632502949 4.10871642662371e-11 -4.67087443281158 1.50848083381937e-55 MirTarget;miRNATAP -0.855521148823258 1.35216036845815e-16 NA NA NA hsa-miR-34a-5p RELN 0.129201805242403 0.133261654068224 -4.67087443281158 1.50848083381937e-55 miRNATAP -0.695025679503336 4.68377105693281e-08 NA NA NA hsa-miR-429 RELN 2.84108892259147 3.88520896426515e-64 -4.67087443281158 1.50848083381937e-55 MirTarget;PITA;miRanda;miRNATAP -0.68086775368573 1.39890314174555e-31 NA NA NA hsa-miR-455-3p RELN 0.678392920628872 0.000327758844555877 -4.67087443281158 1.50848083381937e-55 PITA -0.210673287531595 0.000257471590272544 NA NA NA hsa-miR-589-3p RELN 0.0263457630961427 0.866577983997705 -4.67087443281158 1.50848083381937e-55 MirTarget -0.212301564758921 0.00296952465679882 NA NA NA hsa-miR-590-3p RELN 1.27438512890514 1.03164723481469e-23 -4.67087443281158 1.50848083381937e-55 miRanda;miRNATAP -0.639422421570865 1.47439876580058e-13 NA NA NA hsa-miR-199a-5p RHEB -0.314703593105081 0.00909304983051124 0.313579869806246 6.00889396723587e-07 PITA;miRanda;miRNATAP -0.143553921266532 4.04630951633478e-16 NA NA NA hsa-miR-199b-5p RHEB -1.37022396448377 3.25758057539223e-26 0.313579869806246 6.00889396723587e-07 PITA;miRanda -0.123803296102846 9.10616206978519e-15 NA NA NA hsa-miR-103a-3p RPS6KB1 0.610673390034345 1.63113920514646e-12 -0.0571936206706596 0.498093695192352 miRNAWalker2_validate -0.13730715753257 3.30989618940789e-05 NA NA NA hsa-miR-148a-5p RPS6KB1 0.572692271754695 2.14306251522796e-06 -0.0571936206706596 0.498093695192352 MirTarget;miRNATAP -0.171064566688249 3.39982513466234e-13 NA NA NA hsa-miR-181a-5p RPS6KB1 0.635863607630183 6.72486918157744e-11 -0.0571936206706596 0.498093695192352 MirTarget;miRNATAP -0.130436776786364 8.76017102192071e-06 NA NA NA hsa-miR-181c-5p RPS6KB1 0.574847839298605 4.51287502066478e-07 -0.0571936206706596 0.498093695192352 MirTarget;miRNATAP -0.186058639495696 7.95661299115965e-14 NA NA NA hsa-miR-181d-5p RPS6KB1 0.782618466877349 1.2821116358212e-09 -0.0571936206706596 0.498093695192352 MirTarget -0.164885796389909 5.09783807829002e-14 NA NA NA hsa-miR-218-5p RPS6KB1 -1.02621788318234 2.9221925445771e-23 -0.0571936206706596 0.498093695192352 miRNATAP -0.15390061742019 1.35815707120652e-08 25943352 We found that decreased miR-218 expression in GBM cells also increased the expression of genes encoding additional upstream and downstream components of RTK signaling pathways including the RTK platelet-derived growth factor receptor α PDGFRα and the kinases ribosomal S6 kinase 2 RSK2 and S6 kinase 1 S6K1 that collectively overrode the negative feedback mechanism glioblastoma hsa-miR-223-3p RPS6KB1 -0.966406159689943 7.90612601654887e-12 -0.0571936206706596 0.498093695192352 MirTarget -0.140401561003547 1.98539879754319e-12 NA NA NA hsa-miR-27a-3p RPS6KB1 0.388496361379095 1.42373532086331e-05 -0.0571936206706596 0.498093695192352 MirTarget;miRNATAP -0.152883993564015 2.01871224053452e-06 NA NA NA hsa-miR-30a-5p RPS6KB1 -0.392489994825279 0.00954894667761676 -0.0571936206706596 0.498093695192352 mirMAP;miRNATAP -0.117644522086258 4.71701520431133e-10 NA NA NA hsa-miR-340-5p RPS6KB1 1.0857159627596 4.94471062546756e-24 -0.0571936206706596 0.498093695192352 mirMAP -0.12986566290195 6.5302066469287e-07 NA NA NA hsa-miR-361-5p RPS6KB1 -0.0996867547900457 0.095428250126326 -0.0571936206706596 0.498093695192352 miRanda -0.315724779040643 8.68560752083352e-11 NA NA NA hsa-miR-369-3p RPS6KB1 0.0880848299432269 0.48519534195924 -0.0571936206706596 0.498093695192352 MirTarget;PITA -0.109811993589112 1.74070420680125e-06 NA NA NA hsa-miR-381-3p RPS6KB1 -1.28028930293369 1.61402949866141e-23 -0.0571936206706596 0.498093695192352 MirTarget -0.11550176003524 1.23132177175341e-07 NA NA NA hsa-miR-455-5p RPS6KB1 0.284641224662773 0.0357572339594277 -0.0571936206706596 0.498093695192352 MirTarget;PITA;miRanda -0.195839125781244 5.6227516619354e-21 NA NA NA hsa-miR-542-3p RPS6KB1 0.568822103820889 8.5699075257651e-07 -0.0571936206706596 0.498093695192352 miRNATAP -0.146106785368595 3.33819415150885e-09 NA NA NA hsa-miR-125a-5p RPS6KB2 -0.635694700253268 3.6703235721638e-11 1.06459449292244 1.07488926981491e-32 miRanda -0.276064765205245 1.34823621720886e-17 NA NA NA hsa-miR-26b-5p RPS6KB2 -0.331527896896866 0.000551258576575092 1.06459449292244 1.07488926981491e-32 miRNAWalker2_validate -0.119704281563459 0.00031805074232755 NA NA NA hsa-miR-125b-5p RPTOR -1.94393269763542 2.78180543229264e-58 0.16829263588005 0.00702665954528763 mirMAP -0.11753402336419 4.95913801443251e-13 NA NA NA hsa-miR-1976 RXRA 0.289190368864725 0.00167092758216584 -0.26138464086707 0.000509224921230943 MirTarget -0.173776735702072 4.69820795335018e-10 NA NA NA hsa-miR-423-3p RXRA 0.338351552541003 0.000947983901628776 -0.26138464086707 0.000509224921230943 miRNAWalker2_validate -0.179629348092315 5.82271525887349e-13 NA NA NA hsa-miR-574-3p RXRA -0.797230714010546 1.13016535722548e-14 -0.26138464086707 0.000509224921230943 MirTarget;PITA -0.113625881842827 3.82092527756912e-06 NA NA NA hsa-miR-107 SGK1 0.678998026350662 2.56224148930538e-16 -1.38705395296005 1.03996119610091e-35 miRanda -0.30588649112444 7.82662640380512e-11 NA NA NA hsa-miR-15a-5p SGK1 0.812445324157528 4.50263417099822e-20 -1.38705395296005 1.03996119610091e-35 miRNATAP -0.22757008103211 2.24155024993129e-07 NA NA NA hsa-miR-15b-5p SGK1 0.788659356634696 1.13678211092534e-11 -1.38705395296005 1.03996119610091e-35 miRNATAP -0.121585450525022 0.000333367274223973 NA NA NA hsa-miR-16-5p SGK1 0.46835991343556 1.91125356547776e-06 -1.38705395296005 1.03996119610091e-35 miRNATAP -0.142899579776739 0.000404323817950751 NA NA NA hsa-miR-342-3p SGK1 1.21944481975657 6.81914373001202e-19 -1.38705395296005 1.03996119610091e-35 miRanda -0.224335481296603 9.44361279058632e-16 NA NA NA hsa-miR-590-3p SGK1 1.27438512890514 1.03164723481469e-23 -1.38705395296005 1.03996119610091e-35 miRanda;mirMAP -0.102477113846539 0.00132164401968109 NA NA NA hsa-miR-330-5p SGK2 0.626063225953928 3.42215028606779e-09 -3.71309018975174 5.51926429001233e-61 miRanda -0.63792289435375 1.12808888491018e-16 NA NA NA hsa-miR-21-5p SGK3 2.32007501846389 3.08394781855554e-137 -0.122969610682381 0.394389715112252 miRNAWalker2_validate -0.123722166946783 0.00594242169029888 NA NA NA hsa-miR-582-5p SGK3 0.201134299470983 0.149482495292227 -0.122969610682381 0.394389715112252 miRNATAP -0.159888958983747 7.74861030818091e-06 NA NA NA hsa-miR-590-5p SGK3 0.746515607868274 8.51300479640125e-09 -0.122969610682381 0.394389715112252 PITA;miRanda -0.135326908011393 0.000348089474178509 NA NA NA hsa-miR-107 SOS1 0.678998026350662 2.56224148930538e-16 -0.877855751541721 1.03695500778597e-32 miRNATAP -0.147394654065725 2.31192726053177e-06 NA NA NA hsa-miR-16-1-3p SOS1 1.30142784811196 2.53343713753744e-29 -0.877855751541721 1.03695500778597e-32 mirMAP -0.107043157753765 3.80189774791566e-06 NA NA NA hsa-miR-181a-5p SOS1 0.635863607630183 6.72486918157744e-11 -0.877855751541721 1.03695500778597e-32 MirTarget;miRNATAP -0.159742255037842 1.79079079153389e-09 NA NA NA hsa-miR-181b-5p SOS1 1.22749257795094 1.21998198830919e-27 -0.877855751541721 1.03695500778597e-32 MirTarget;miRNATAP -0.145535984933139 7.83280749467566e-11 NA NA NA hsa-miR-181c-5p SOS1 0.574847839298605 4.51287502066478e-07 -0.877855751541721 1.03695500778597e-32 MirTarget;miRNATAP -0.128681631990538 1.68288122680838e-08 NA NA NA hsa-miR-181d-5p SOS1 0.782618466877349 1.2821116358212e-09 -0.877855751541721 1.03695500778597e-32 MirTarget -0.116569643832434 6.10109884911499e-09 NA NA NA hsa-miR-182-5p SOS1 2.36408084103818 2.60011457600281e-72 -0.877855751541721 1.03695500778597e-32 miRNATAP -0.133258609259375 1.37594627633909e-13 NA NA NA hsa-miR-361-5p SOS1 -0.0996867547900457 0.095428250126326 -0.877855751541721 1.03695500778597e-32 miRanda -0.165292469967548 0.000212244095536951 NA NA NA hsa-miR-429 SOS1 2.84108892259147 3.88520896426515e-64 -0.877855751541721 1.03695500778597e-32 miRanda;miRNATAP -0.114619423820961 9.86568637608249e-16 NA NA NA hsa-miR-542-3p SOS1 0.568822103820889 8.5699075257651e-07 -0.877855751541721 1.03695500778597e-32 miRanda -0.156663695721104 2.52964146595814e-12 NA NA NA hsa-miR-96-5p SOS1 3.24596558166606 1.92296159041707e-98 -0.877855751541721 1.03695500778597e-32 TargetScan;miRNATAP -0.114957974727393 1.33288008849377e-14 NA NA NA hsa-miR-130b-3p SOS2 1.38122163823846 1.00932179737836e-26 -0.693584357428233 2.85648006434693e-23 miRNATAP -0.109371088184118 2.82016388666753e-09 NA NA NA hsa-miR-146b-5p SOS2 0.631041661167954 6.20366593452965e-08 -0.693584357428233 2.85648006434693e-23 miRanda -0.18410115041905 2.89003141851973e-19 NA NA NA hsa-miR-15a-5p SOS2 0.812445324157528 4.50263417099822e-20 -0.693584357428233 2.85648006434693e-23 miRNATAP -0.260459131538532 1.04889039594078e-22 NA NA NA hsa-miR-21-5p SOS2 2.32007501846389 3.08394781855554e-137 -0.693584357428233 2.85648006434693e-23 miRNATAP -0.267389788495656 7.45996997519246e-36 NA NA NA hsa-miR-139-5p SPP1 -3.25846663863766 2.62018989415003e-112 2.26670631164277 1.22530665713086e-25 miRanda -0.500352860676903 2.02284408020613e-29 NA NA NA hsa-miR-335-5p SPP1 -2.61355235628987 2.6462884165886e-51 2.26670631164277 1.22530665713086e-25 miRNAWalker2_validate -0.324190847145869 2.06671050183243e-15 NA NA NA hsa-miR-34c-5p SPP1 -0.0896438810345765 0.557016551646841 2.26670631164277 1.22530665713086e-25 miRanda -0.285906011260852 1.12986821005389e-08 NA NA NA hsa-miR-199a-3p STK11 -0.174830922802531 0.138748242352612 0.544842212403913 5.57417996369907e-11 PITA;miRNATAP -0.209034875931642 2.73576834835512e-18 NA NA NA hsa-miR-199b-3p STK11 -0.173412359075504 0.143232985227463 0.544842212403913 5.57417996369907e-11 PITA -0.209157826415224 2.02086037375367e-18 NA NA NA hsa-miR-326 STK11 -1.54449030848141 1.04703417845594e-17 0.544842212403913 5.57417996369907e-11 miRanda -0.166560489246979 4.2942013137581e-28 NA NA NA hsa-miR-330-5p STK11 0.626063225953928 3.42215028606779e-09 0.544842212403913 5.57417996369907e-11 miRanda -0.150358933571276 2.12169768579404e-08 NA NA NA hsa-miR-125a-3p TEK -0.176412960953959 0.10108079286419 -2.06294068128163 1.56421351499436e-50 miRanda -0.160800153949765 0.000632524010983298 NA NA NA hsa-miR-455-5p TEK 0.284641224662773 0.0357572339594277 -2.06294068128163 1.56421351499436e-50 miRanda -0.286824311750872 5.09302613807658e-15 NA NA NA hsa-miR-29b-2-5p TGFA -0.263499971180298 0.0400001755611965 -0.858787895391806 9.79578648139949e-07 mirMAP -0.338031086497945 4.06455756481292e-13 NA NA NA hsa-miR-342-3p TGFA 1.21944481975657 6.81914373001202e-19 -0.858787895391806 9.79578648139949e-07 miRanda -0.21750744009064 3.98827798617966e-07 NA NA NA hsa-miR-155-5p THBS1 1.09766314984151 4.55650930885814e-15 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate;mirMAP -0.133542323510001 7.64645600006924e-06 NA NA NA hsa-miR-16-1-3p THBS1 1.30142784811196 2.53343713753744e-29 -0.0614785645873859 0.62138763262321 MirTarget -0.140131478293771 0.000198601030104097 NA NA NA hsa-miR-17-5p THBS1 0.599531757298529 1.07318660415906e-05 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.211166755871651 7.35327505985432e-12 NA NA NA hsa-miR-181a-5p THBS1 0.635863607630183 6.72486918157744e-11 -0.0614785645873859 0.62138763262321 mirMAP -0.150019822568732 0.000545990600769292 NA NA NA hsa-miR-181b-5p THBS1 1.22749257795094 1.21998198830919e-27 -0.0614785645873859 0.62138763262321 mirMAP -0.1533233095909 2.76267327611462e-05 NA NA NA hsa-miR-181c-5p THBS1 0.574847839298605 4.51287502066478e-07 -0.0614785645873859 0.62138763262321 mirMAP -0.147290024790751 7.4630964763336e-05 NA NA NA hsa-miR-18a-5p THBS1 0.969066793715868 1.69069796425892e-08 -0.0614785645873859 0.62138763262321 MirTarget -0.181152396860618 8.69562380186631e-14 NA NA NA hsa-miR-19a-3p THBS1 0.817507952171848 3.76285090469625e-08 -0.0614785645873859 0.62138763262321 MirTarget;mirMAP -0.214042484472341 2.35179044480412e-14 NA NA NA hsa-miR-19b-3p THBS1 0.139124391010031 0.255658553872934 -0.0614785645873859 0.62138763262321 MirTarget;mirMAP -0.25361005913269 1.79395476818e-13 NA NA NA hsa-miR-20a-5p THBS1 0.44979911120222 0.000602386964327228 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.30952947364433 1.36418642869234e-22 NA NA NA hsa-miR-32-5p THBS1 1.38676603945192 2.29428299685976e-34 -0.0614785645873859 0.62138763262321 mirMAP -0.19932792247749 2.72222895176776e-08 NA NA NA hsa-miR-455-5p THBS1 0.284641224662773 0.0357572339594277 -0.0614785645873859 0.62138763262321 miRanda -0.181790591068211 5.96114242364848e-09 NA NA NA hsa-miR-500a-5p THBS1 0.209805801129289 0.119177819862398 -0.0614785645873859 0.62138763262321 mirMAP -0.167786316949011 1.51589228334337e-07 NA NA NA hsa-miR-590-3p THBS1 1.27438512890514 1.03164723481469e-23 -0.0614785645873859 0.62138763262321 miRanda -0.175386342682792 2.46112499417128e-07 NA NA NA hsa-miR-590-5p THBS1 0.746515607868274 8.51300479640125e-09 -0.0614785645873859 0.62138763262321 miRanda;mirMAP -0.167667995453039 2.43506908893591e-07 NA NA NA hsa-miR-671-5p THBS1 1.55248047544015 7.7377784125886e-33 -0.0614785645873859 0.62138763262321 MirTarget -0.149992236442967 2.76078556306308e-06 NA NA NA hsa-miR-92a-3p THBS1 -0.342420481092807 0.000732275969501111 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.236223996163107 1.60863345703223e-08 NA NA NA hsa-miR-98-5p THBS1 0.656336869298973 9.03113188590466e-15 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.196108417985267 8.06107193430326e-05 NA NA NA hsa-miR-106b-5p THBS2 1.22477789913175 2.31787213143326e-32 0.769808163205687 9.78636441866493e-07 MirTarget -0.302711566656831 1.58196287774772e-09 NA NA NA hsa-miR-139-5p THBS2 -3.25846663863766 2.62018989415003e-112 0.769808163205687 9.78636441866493e-07 miRanda -0.18351393710485 1.38608699702018e-08 NA NA NA hsa-miR-16-2-3p THBS2 0.286355254959005 0.0256946046884474 0.769808163205687 9.78636441866493e-07 mirMAP -0.266853998218325 2.47345099228546e-10 NA NA NA hsa-miR-17-5p THBS2 0.599531757298529 1.07318660415906e-05 0.769808163205687 9.78636441866493e-07 MirTarget;TargetScan -0.253625690199161 1.06814941199192e-10 NA NA NA hsa-miR-20a-5p THBS2 0.44979911120222 0.000602386964327228 0.769808163205687 9.78636441866493e-07 MirTarget -0.342590656166951 2.95705202030977e-17 NA NA NA hsa-miR-29a-3p THBS2 -0.699539666833076 1.13935003139957e-13 0.769808163205687 9.78636441866493e-07 MirTarget -0.330180674500405 5.19990176669046e-09 NA NA NA hsa-miR-29b-3p THBS2 0.438847962603729 6.46722433236121e-05 0.769808163205687 9.78636441866493e-07 MirTarget -0.440296132980001 7.26973369809758e-20 NA NA NA hsa-miR-29c-3p THBS2 -0.0652732378039769 0.563774115756922 0.769808163205687 9.78636441866493e-07 MirTarget -0.431990941588527 4.39914608500664e-20 NA NA NA hsa-miR-320a THBS2 -0.287921276129346 0.00141693637596294 0.769808163205687 9.78636441866493e-07 mirMAP -0.208219274662831 0.000550427836846861 NA NA NA hsa-miR-320b THBS2 0.412787504747938 0.00113457276903985 0.769808163205687 9.78636441866493e-07 mirMAP -0.124670374856683 0.00370734965981903 NA NA NA hsa-miR-335-3p THBS2 -1.88448538773649 2.09538234398335e-32 0.769808163205687 9.78636441866493e-07 MirTarget;mirMAP -0.104434722339321 0.00143160463399029 NA NA NA hsa-miR-590-3p THBS2 1.27438512890514 1.03164723481469e-23 0.769808163205687 9.78636441866493e-07 miRanda;mirMAP -0.165183001603145 0.000140989548326802 NA NA NA hsa-miR-590-5p THBS2 0.746515607868274 8.51300479640125e-09 0.769808163205687 9.78636441866493e-07 miRanda -0.184580610139702 8.27333563753023e-06 NA NA NA hsa-miR-7-1-3p THBS2 0.686579567507582 1.20731554448806e-07 0.769808163205687 9.78636441866493e-07 MirTarget;mirMAP -0.1868408017881 6.41716930046462e-06 NA NA NA hsa-miR-93-5p THBS2 1.01788600209314 1.24312921618126e-21 0.769808163205687 9.78636441866493e-07 MirTarget -0.273544363928753 3.16076912551438e-08 NA NA NA hsa-miR-326 THBS3 -1.54449030848141 1.04703417845594e-17 0.245441799654582 0.00263276060533888 PITA -0.120857065691596 6.87702237562674e-16 NA NA NA hsa-miR-142-3p THBS4 1.53951237846104 2.06769936282034e-17 -0.661382745748392 0.00786792397096919 miRanda -0.240044523261729 1.6805188940302e-07 NA NA NA hsa-miR-320b THBS4 0.412787504747938 0.00113457276903985 -0.661382745748392 0.00786792397096919 miRanda -0.430826287964397 1.28903705137441e-10 NA NA NA hsa-miR-107 TLR4 0.678998026350662 2.56224148930538e-16 -1.6490372510276 1.59824287329687e-47 miRanda -0.314192687515606 1.35045556955962e-10 NA NA NA hsa-miR-17-5p TLR4 0.599531757298529 1.07318660415906e-05 -1.6490372510276 1.59824287329687e-47 TargetScan -0.229180792527581 1.62807865459736e-14 NA NA NA hsa-miR-21-5p TLR4 2.32007501846389 3.08394781855554e-137 -1.6490372510276 1.59824287329687e-47 miRNAWalker2_validate -0.359793062620959 1.10927793812338e-22 27286259 For in vivo relevance expression of TLR4 was correlated with miR-21 expression and ROS production in freshly isolated untreated primary human lung cancer cells lung cancer hsa-miR-331-5p TLR4 0.682437632502949 4.10871642662371e-11 -1.6490372510276 1.59824287329687e-47 MirTarget -0.177454399821036 7.95152912181529e-06 NA NA NA hsa-miR-590-3p TLR4 1.27438512890514 1.03164723481469e-23 -1.6490372510276 1.59824287329687e-47 miRanda;mirMAP -0.153708141758483 3.37312311034582e-06 NA NA NA hsa-miR-590-5p TLR4 0.746515607868274 8.51300479640125e-09 -1.6490372510276 1.59824287329687e-47 miRanda -0.163934537866181 2.05626813690214e-07 NA NA NA hsa-miR-365a-3p TNC -1.45032725192851 2.37888098061118e-37 -0.13661350853256 0.448221214380957 MirTarget -0.138940803738652 0.00756635738729108 NA NA NA hsa-miR-125a-3p TNN -0.176412960953959 0.10108079286419 -1.54208094497544 8.25161343787378e-11 miRanda -0.374219624881681 1.02778952969851e-06 NA NA NA hsa-miR-338-3p TNN 0.0799903526884966 0.575137668326679 -1.54208094497544 8.25161343787378e-11 miRanda -0.26256563605259 5.09145719630248e-06 NA NA NA hsa-miR-1301-3p TNXB 1.80754972811286 2.96809840886499e-36 -4.29695121841169 1.00427411586091e-87 miRNAWalker2_validate -0.838795674126697 2.37686650012749e-60 NA NA NA hsa-miR-3607-3p TNXB 0.00697875745616461 0.972073275173643 -4.29695121841169 1.00427411586091e-87 miRNATAP -0.263395946229919 1.61191128010848e-10 NA NA NA hsa-miR-106b-5p TSC1 1.22477789913175 2.31787213143326e-32 -0.538880909055464 1.58533734753933e-21 miRNAWalker2_validate -0.137231047196163 5.24123367568988e-14 NA NA NA hsa-miR-148a-5p TSC1 0.572692271754695 2.14306251522796e-06 -0.538880909055464 1.58533734753933e-21 mirMAP -0.103146356844004 1.92663861875026e-10 NA NA NA hsa-miR-155-5p TSC1 1.09766314984151 4.55650930885814e-15 -0.538880909055464 1.58533734753933e-21 mirMAP -0.137386166665064 3.86179843967193e-24 NA NA NA hsa-miR-340-5p TSC1 1.0857159627596 4.94471062546756e-24 -0.538880909055464 1.58533734753933e-21 mirMAP -0.107750374208541 1.62313786574761e-09 NA NA NA hsa-miR-101-3p VEGFA -0.126035286271986 0.145997832808138 0.558196398264416 4.82412858140541e-08 miRNAWalker2_validate -0.244521682678937 1.84891665047835e-09 NA NA NA hsa-miR-134-5p VEGFA -0.943054080774898 1.50079821035544e-12 0.558196398264416 4.82412858140541e-08 miRNAWalker2_validate;miRTarBase;MirTarget -0.167128564426745 1.10726961805336e-10 NA NA NA hsa-miR-150-5p VEGFA -0.832049526091211 3.45299201139224e-05 0.558196398264416 4.82412858140541e-08 miRNAWalker2_validate;miRTarBase -0.151371862718652 1.00198904514709e-18 NA NA NA hsa-miR-195-5p VEGFA -1.47508108420708 2.20685847368258e-38 0.558196398264416 4.82412858140541e-08 miRNAWalker2_validate;miRTarBase;MirTarget -0.218575701645509 7.55403373439709e-14 27574422;23468064;26823724 In this study we used a new cationic peptide disulfide cross-linked stearylated polyarginine peptide modified with histidine H3R5 as a reducible vector cell penetrating peptide-modified aptamer ST21 with specific binding to HCC cells to conjugate to peptide H3R5 as the targeting probe miRNA-195 miR195 as a powerful gene drug to inhibit VEGF and fasudil to suppress vasculogenic mimicry by blocking ROCK2 all of which were simultaneously encapsulated in the same nanoparticles;Furthermore we revealed that miR-195 down-regulation resulted in enhanced VEGF levels in the tumor microenvironment which subsequently activated VEGF receptor 2 signaling in endothelial cells and thereby promoted angiogenesis;MiR 195 is a key negative regulator of hepatocellular carcinoma metastasis by targeting FGF2 and VEGFA; Luciferase reporter and ELISA assay prove that hematogenous metastasis related genes including FGF2 and VEGFA are the target genes of miR-195; Taken together our results suggest that miR-195 a tumor suppressor miRNA contributes to the lung metastasis of HCC by negatively regulating FGF2 and VEGFA providing key implications of miR-195 for the therapeutic intervention of HCC ;;metastasis liver cancer;liver cancer;liver cancer hsa-miR-199a-5p VEGFA -0.314703593105081 0.00909304983051124 0.558196398264416 4.82412858140541e-08 miRanda;miRNATAP -0.179279483927549 6.72134651164296e-10 25444917 In addition co-transfection with miR-199a mimic reversed the CCL5-mediated VEGF expression and angiogenesis in vitro and in vivo sarcoma hsa-miR-199b-5p VEGFA -1.37022396448377 3.25758057539223e-26 0.558196398264416 4.82412858140541e-08 miRanda -0.228409524155996 1.33793206666423e-18 NA NA NA hsa-miR-29a-3p VEGFA -0.699539666833076 1.13935003139957e-13 0.558196398264416 4.82412858140541e-08 MirTarget -0.114785243382774 0.00196474782587542 24209632 miR 29a suppresses growth and invasion of gastric cancer cells in vitro by targeting VEGF A; Furthermore western blot combined with the luciferase reporter assays demonstrate that vascular endothelial growth factor A VEGF-A is direct target of miR-29a; This is the first time miR-29a was found to suppress the tumor microvessel density in gastric cancer by targeting VEGF-A gastric cancer hsa-miR-29c-3p VEGFA -0.0652732378039769 0.563774115756922 0.558196398264416 4.82412858140541e-08 miRNAWalker2_validate;MirTarget -0.126355371943564 5.32172855437951e-05 NA NA NA hsa-miR-335-5p VEGFA -2.61355235628987 2.6462884165886e-51 0.558196398264416 4.82412858140541e-08 miRNAWalker2_validate -0.103950468396491 4.59347799365143e-08 NA NA NA hsa-miR-497-5p VEGFA -1.4667331575679 5.49909921822638e-36 0.558196398264416 4.82412858140541e-08 MirTarget -0.126722638387264 1.08881401780323e-05 26718330;26485685;26336827;26202364;27015364;25176450;26840372 Furthermore western blot assay confirmed that the overexpression of miR-497 reduced VEGF and HIF-1α protein levels; Moreover miR-497 inhibited the growth of tumors and reduced angiogenesis in a nude mouse xenograft tumor model which was probably caused by the downregulation of pro-angiogenic molecules such as VEGF and HIF-1α;Role of miR 497 in VEGF A mediated cancer cell growth and invasion in non small cell lung cancer; Here we showed that compared to other cancers NSCLC had a significant higher ratio of VEGF-A protein vs mRNA and significantly lower levels of miR-497 suggesting presence of a post-transcriptional control of VEGF-A in NSCLC different from other cancers; Compared with paired normal lung tissue NSCLC expressed lower levels of miR-497 and higher levels of VEGF-A; Moreover the levels of miR-497 and VEGF-A were inversely correlated in NSCLC specimen; Bioinformatics analyses showed that miR-497 bound to 3'-UTR of VEGF-A mRNA in NSCLC lines to inhibit its translation; Overexpression of miR-497 in NSCLC lines decreased VEGF-A protein while depletion of miR-497 in NSCLC lines increased VEGF-A protein; However modification of miR-497 levels in NSCLC lines did not alter VEGF-A mRNA levels;MiR 497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG 1; Subsequent investigations disclosed that miR-497 directly inhibited the 3'-untranslated regions UTRs of vascular endothelial growth factor A VEGFA and astrocyte elevated gene-1 AEG-1;The effects of ectopic miR-497 expression on the expression of VEGFA Akt and p-Akt were determined by western blot; Luciferase reporter assay and western blot confirmed that VEGFA was a direct target of miR-497;Subsequent investigations disclosed that Twist was regulated by miR-497 directly leading to the increased level of Vascular Endothelial Growth Factor-A VEGFA;We further disclosed that miR-497 exerted its function of anti-angiogenesis by suppressing VEGFA expression in ovarian cancer cells and in turn impairing the VEGFR2-mediated PI3K/AKT and MAPK/ERK pathways;Vascular endothelial growth factor-A VEGF-A was confirmed to be a target of miR-497 ;;metastasis;;;; breast cancer;lung squamous cell cancer;liver cancer;sarcoma;pancreatic cancer;ovarian cancer;colorectal cancer hsa-miR-128-3p VEGFC 0.90296715955238 9.925204823863e-23 -0.815181679329146 2.46618490633351e-18 miRNAWalker2_validate;MirTarget -0.277923499247748 3.92415081523196e-16 25001183;26722309 Interestingly ectopic miR-128 overexpression could significantly inhibit vascular endothelial growth factor VEGF-C expression and reduce the activity of a luciferase reporter containing the VEGF-C 3'-untranslated region; These findings suggest that miR-128 could play a role in NSCLC tumourigenesis at least in part by modulation of angiogenesis and lymphangiogenesis through targeting VEGF-C and could simultaneously block ERK AKT and p38 signalling pathways;miR 128 downregulation promotes growth and metastasis of bladder cancer cells and involves VEGF C upregulation; To investigate the regulation of miR-128 on VEGF-C expression and their effects on proliferation and metastasis of bladder cancer T24 and 5637 BC cells were transfected with pre-miR-128 anti-miR-128 and their respective negative control; miR-128 was downregulated in BC tissues and cell lines while the expression levels of VEGF-C were upregulated; The present results indicated that miR-128 negatively regulated VEGF-C expression in BC T24 and 5637 BC cells; VEGF-C is a direct target of miR-128 in BC cells ;metastasis lung squamous cell cancer;bladder cancer hsa-miR-16-2-3p VEGFC 0.286355254959005 0.0256946046884474 -0.815181679329146 2.46618490633351e-18 MirTarget -0.165282903433736 7.39907917672124e-11 NA NA NA hsa-miR-590-3p VEGFC 1.27438512890514 1.03164723481469e-23 -0.815181679329146 2.46618490633351e-18 miRanda -0.194896362906364 4.34747156563496e-14 NA NA NA hsa-miR-15a-5p VWF 0.812445324157528 4.50263417099822e-20 -2.03096525307727 9.93612805244949e-62 mirMAP -0.31151716601614 4.32266830697716e-10 NA NA NA hsa-miR-15b-5p VWF 0.788659356634696 1.13678211092534e-11 -2.03096525307727 9.93612805244949e-62 mirMAP -0.352554809474714 1.06468496540939e-20 NA NA NA hsa-miR-16-5p VWF 0.46835991343556 1.91125356547776e-06 -2.03096525307727 9.93612805244949e-62 mirMAP -0.307576833437167 1.52179655185271e-11 NA NA NA hsa-miR-429 VWF 2.84108892259147 3.88520896426515e-64 -2.03096525307727 9.93612805244949e-62 miRNATAP -0.368905568901854 5.44840907856256e-56 NA NA NA hsa-miR-218-5p YWHAB -1.02621788318234 2.9221925445771e-23 0.367736029126437 1.14326902549126e-12 MirTarget;miRNATAP -0.118771072777071 1.2025708808827e-12 NA NA NA hsa-miR-361-5p YWHAB -0.0996867547900457 0.095428250126326 0.367736029126437 1.14326902549126e-12 MirTarget;PITA;miRanda;miRNATAP -0.129790382733719 1.94479562657361e-05 NA NA NA hsa-miR-26a-5p YWHAE -0.481180084102235 1.82368246174758e-10 0.0789078821698794 0.139244898488797 miRNAWalker2_validate;miRNATAP -0.139062156125765 6.21839958682607e-09 NA NA NA hsa-miR-181c-5p YWHAG 0.574847839298605 4.51287502066478e-07 -0.30496627096666 3.16855379814272e-06 MirTarget;miRNATAP -0.145586859940849 6.31211340218828e-14 NA NA NA hsa-miR-181d-5p YWHAG 0.782618466877349 1.2821116358212e-09 -0.30496627096666 3.16855379814272e-06 MirTarget -0.103217848799385 1.84401651957152e-09 NA NA NA hsa-miR-200b-3p YWHAG 2.11744072703209 1.66710572104191e-43 -0.30496627096666 3.16855379814272e-06 MirTarget;TargetScan -0.11952750278469 2.04909339347489e-18 NA NA NA hsa-miR-200c-3p YWHAG 2.07038651806183 6.84613386286599e-51 -0.30496627096666 3.16855379814272e-06 MirTarget;miRNATAP -0.115098762293347 2.44122702999837e-14 NA NA NA hsa-miR-30a-5p YWHAG -0.392489994825279 0.00954894667761676 -0.30496627096666 3.16855379814272e-06 miRNATAP -0.130408882982876 3.06189502357674e-19 NA NA NA hsa-miR-34c-3p YWHAG 0.213037310586265 0.144107566901292 -0.30496627096666 3.16855379814272e-06 MirTarget;PITA -0.101761318229923 6.09583697264274e-11 NA NA NA hsa-miR-361-3p YWHAG -0.0370446538644886 0.624902392829872 -0.30496627096666 3.16855379814272e-06 miRNATAP -0.123033020131908 3.98096255501155e-05 NA NA NA hsa-miR-195-5p YWHAH -1.47508108420708 2.20685847368258e-38 0.574547913335089 6.31677198141686e-23 MirTarget;miRNATAP -0.154571273062705 3.79154556881082e-20 NA NA NA hsa-miR-361-5p YWHAH -0.0996867547900457 0.095428250126326 0.574547913335089 6.31677198141686e-23 miRanda -0.122118229637353 0.00045156556380787 NA NA NA hsa-miR-497-5p YWHAH -1.4667331575679 5.49909921822638e-36 0.574547913335089 6.31677198141686e-23 MirTarget;miRNATAP -0.101887170785079 8.76599010415193e-10 NA NA NA hsa-miR-195-5p YWHAQ -1.47508108420708 2.20685847368258e-38 0.14614608366116 0.0210037506575042 MirTarget;miRNATAP -0.131078204286072 2.84212998538795e-13 NA NA NA hsa-let-7b-5p YWHAZ -0.54215483448257 7.21347617597858e-08 0.614503695418861 6.69168275891896e-13 miRNAWalker2_validate -0.143693415048529 9.14380074121916e-07 NA NA NA hsa-miR-140-3p YWHAZ -1.25844395244141 5.29222651039892e-33 0.614503695418861 6.69168275891896e-13 MirTarget;PITA;miRNATAP -0.220682872816636 1.53890225766904e-16 NA NA NA hsa-miR-195-3p YWHAZ -1.49582726452416 6.53261924105147e-40 0.614503695418861 6.69168275891896e-13 MirTarget -0.217318055382634 8.6510493117177e-19 NA NA NA hsa-miR-199a-5p YWHAZ -0.314703593105081 0.00909304983051124 0.614503695418861 6.69168275891896e-13 miRanda -0.142636123966747 5.50463507912081e-09 NA NA NA hsa-miR-199b-5p YWHAZ -1.37022396448377 3.25758057539223e-26 0.614503695418861 6.69168275891896e-13 miRanda -0.177217586869737 5.92745522971954e-16 NA NA NA hsa-miR-30a-5p YWHAZ -0.392489994825279 0.00954894667761676 0.614503695418861 6.69168275891896e-13 miRNAWalker2_validate;MirTarget;miRNATAP -0.183528380744408 8.33049950582494e-22 NA NA NA hsa-miR-34c-5p YWHAZ -0.0896438810345765 0.557016551646841 0.614503695418861 6.69168275891896e-13 miRanda -0.122122109832999 2.94590640777635e-10 NA NA NA hsa-miR-495-3p YWHAZ -1.67685437612894 1.70634429769052e-34 0.614503695418861 6.69168275891896e-13 mirMAP -0.129022570922436 4.47948910436123e-10 NA NA NA