miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-200b-3p ABCA1 2.06025765178663 3.07718049337497e-10 0.607204161288785 0.000197686022710701 TargetScan -0.163863464161358 1.5547472437985e-08 NA NA NA hsa-miR-192-5p ABCA8 2.0867723747501 3.57854249239079e-08 -2.99190082801217 2.10585396600667e-10 miRNAWalker2_validate -0.488500191150211 4.11167345797026e-11 NA NA NA hsa-miR-200b-3p ABI2 2.06025765178663 3.07718049337497e-10 0.175334214271024 0.14786862779391 TargetScan -0.168666434337723 2.27882307029266e-16 NA NA NA hsa-miR-21-5p ACAT1 2.21515015440792 6.0468197386674e-49 -0.962552552536598 5.9887145919487e-14 miRNAWalker2_validate -0.323427434087958 1.30635045869468e-13 NA NA NA hsa-miR-192-5p ACSL1 2.0867723747501 3.57854249239079e-08 -0.584863548769266 4.27832126568988e-05 miRNAWalker2_validate -0.112204276874451 4.77898049716326e-07 NA NA NA hsa-miR-200b-3p ACTR1A 2.06025765178663 3.07718049337497e-10 -0.397609071151318 1.89148917623928e-07 TargetScan -0.108111914069706 2.12268301213487e-16 NA NA NA hsa-miR-200b-3p ADAMTS3 2.06025765178663 3.07718049337497e-10 0.381745529837852 0.0472261904397674 TargetScan -0.180784168617438 1.04752758962753e-07 NA NA NA hsa-miR-200b-3p ADCY2 2.06025765178663 3.07718049337497e-10 -1.52957881268437 3.68996645479601e-05 TargetScan -0.621291864739548 1.72587422423198e-23 NA NA NA hsa-miR-200b-3p ADCY9 2.06025765178663 3.07718049337497e-10 -0.36257850343414 0.0191055880639231 TargetScan -0.214341971832769 4.89518535816012e-16 NA NA NA hsa-miR-200b-3p AFF1 2.06025765178663 3.07718049337497e-10 -0.38392701055879 0.000534832405102977 TargetScan -0.135739567609764 1.9152841266992e-12 NA NA NA hsa-miR-200b-3p AFF3 2.06025765178663 3.07718049337497e-10 -2.37331346400795 1.1482078568002e-10 TargetScan -0.563896340686397 2.42200688738896e-18 NA NA NA hsa-miR-18a-5p AFF4 2.04701000113742 1.30593450319289e-12 -0.266238435778205 0.0156473179650327 miRNAWalker2_validate -0.148452075199908 4.41395051368717e-12 NA NA NA hsa-miR-200b-3p AHNAK 2.06025765178663 3.07718049337497e-10 -1.2069240076455 1.34650096153062e-09 TargetScan -0.315757295187101 5.23497379185866e-20 NA NA NA hsa-miR-183-5p AKAP12 2.08412478942637 1.08931431254487e-10 -0.931840498501576 0.00280002417909614 miRNAWalker2_validate;miRTarBase -0.573047168308188 7.07407121257084e-29 NA NA NA hsa-miR-21-5p AKT2 2.21515015440792 6.0468197386674e-49 -0.163157777800721 0.0933816097673111 miRNAWalker2_validate -0.125319232582453 0.000121164702766457 NA NA NA hsa-miR-146b-5p AKT3 2.23105707690238 3.83997814011313e-23 -0.273001052722672 0.188651193580836 miRNAWalker2_validate -0.255166569260379 5.2556904982786e-07 NA NA NA hsa-miR-200b-3p AMOTL2 2.06025765178663 3.07718049337497e-10 0.146193942930294 0.30776365924427 TargetScan -0.110709235845408 1.3504727226348e-05 NA NA NA hsa-miR-192-5p ANKRD6 2.0867723747501 3.57854249239079e-08 -0.362693205944883 0.0394241452373118 miRNAWalker2_validate -0.21297280927049 3.86019911992963e-16 NA NA NA hsa-miR-200b-3p ANO5 2.06025765178663 3.07718049337497e-10 -2.17078328848367 8.0164866381684e-07 TargetScan -0.490376267388203 4.32101550307116e-10 NA NA NA hsa-miR-192-5p ANO6 2.0867723747501 3.57854249239079e-08 -0.0852083698444357 0.502269433202532 miRNAWalker2_validate -0.10991132446706 1.35656993437427e-08 NA NA NA hsa-miR-200b-3p ANO6 2.06025765178663 3.07718049337497e-10 -0.0852083698444357 0.502269433202532 TargetScan -0.163682649982031 4.96272712542492e-14 NA NA NA hsa-miR-192-5p AP1S2 2.0867723747501 3.57854249239079e-08 -0.606780926693101 0.00205224777129082 miRNAWalker2_validate -0.339299903270277 7.57738254499468e-36 NA NA NA hsa-miR-200b-3p AP1S2 2.06025765178663 3.07718049337497e-10 -0.606780926693101 0.00205224777129082 TargetScan -0.409677325624998 5.51773958449442e-41 NA NA NA hsa-miR-192-5p APOLD1 2.0867723747501 3.57854249239079e-08 -0.383751863841876 0.0485139708584483 miRNAWalker2_validate -0.128346096274816 2.16150212923558e-05 NA NA NA hsa-miR-21-5p APOLD1 2.21515015440792 6.0468197386674e-49 -0.383751863841876 0.0485139708584483 miRNAWalker2_validate -0.279710503336859 1.91741828890753e-05 NA NA NA hsa-miR-200b-3p ARHGAP20 2.06025765178663 3.07718049337497e-10 -1.37521379031409 6.54964322003788e-07 TargetScan -0.525093352316807 4.62765133109738e-31 NA NA NA hsa-miR-192-5p ARHGAP29 2.0867723747501 3.57854249239079e-08 0.131379720033421 0.429533510526147 miRNAWalker2_validate -0.133997834211656 1.49251889090626e-07 NA NA NA hsa-miR-200b-3p ARHGAP6 2.06025765178663 3.07718049337497e-10 -0.528937130002503 0.0400586038016635 TargetScan -0.294522253525362 5.68779920266667e-11 NA NA NA hsa-miR-192-5p ARHGEF10 2.0867723747501 3.57854249239079e-08 -0.0978946958084785 0.610003579994436 miRNAWalker2_validate -0.174267226405221 2.48276070321823e-09 NA NA NA hsa-miR-200b-3p ARHGEF17 2.06025765178663 3.07718049337497e-10 0.034326144778178 0.858945227172506 TargetScan -0.337457388320201 6.42116541129292e-27 NA NA NA hsa-miR-192-5p ARHGEF26 2.0867723747501 3.57854249239079e-08 -1.95662528882127 5.95096344991847e-08 miRNAWalker2_validate -0.50938422082555 3.08532273084327e-21 NA NA NA hsa-miR-192-5p ARL2BP 2.0867723747501 3.57854249239079e-08 -0.343286861480952 3.24929129460008e-05 miRNAWalker2_validate -0.105115494989654 8.87252420866826e-18 NA NA NA hsa-miR-200b-3p ARL2BP 2.06025765178663 3.07718049337497e-10 -0.343286861480952 3.24929129460008e-05 TargetScan -0.111898694101105 3.38650428950985e-15 NA NA NA hsa-miR-200b-3p ARL3 2.06025765178663 3.07718049337497e-10 -0.521628086406404 2.45297123307517e-07 TargetScan -0.1512050080486 3.28914199335082e-18 NA NA NA hsa-miR-133a-3p ARL6IP1 -2.63342156915404 5.72661351480585e-12 0.577537808488563 1.35778175758293e-05 miRNAWalker2_validate -0.144213438055402 2.66374212433194e-13 NA NA NA hsa-miR-21-5p ARMCX3 2.21515015440792 6.0468197386674e-49 -0.174723325566402 0.294467880066106 miRNAWalker2_validate -0.118942541202785 0.03566440612269 NA NA NA hsa-miR-200b-3p ARRDC3 2.06025765178663 3.07718049337497e-10 -0.146523158782965 0.287414873160273 TargetScan -0.121212288058031 6.12636605988112e-07 NA NA NA hsa-miR-200b-3p ASXL3 2.06025765178663 3.07718049337497e-10 -1.87842198623791 7.63940713243169e-08 TargetScan -0.455451974562606 1.67265650550218e-13 NA NA NA hsa-miR-200b-3p ATL2 2.06025765178663 3.07718049337497e-10 -0.383786242998031 0.000575339633888933 TargetScan -0.144410609905955 6.55387849763679e-14 NA NA NA hsa-miR-18a-5p ATM 2.04701000113742 1.30593450319289e-12 0.177849811817311 0.2156767159682 miRNAWalker2_validate;miRTarBase -0.106088412949694 0.000233162954700209 23437304;25963391;23857602;23229340 MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression colorectal cancer;cervical and endocervical cancer;breast cancer;colon cancer hsa-miR-192-5p ATP10D 2.0867723747501 3.57854249239079e-08 -0.370662483390109 0.00764032733906965 miRNAWalker2_validate -0.118803952333699 2.63436462290521e-08 NA NA NA hsa-miR-21-5p ATP2B4 2.21515015440792 6.0468197386674e-49 -0.644860700805612 0.00597426739858389 miRNAWalker2_validate -0.49658752508725 1.53394775911162e-10 NA NA NA hsa-miR-192-5p ATRNL1 2.0867723747501 3.57854249239079e-08 -1.68925010520238 1.18504412004834e-05 miRNAWalker2_validate -0.332444919500384 3.2567358192866e-08 NA NA NA hsa-miR-200b-3p ATXN1 2.06025765178663 3.07718049337497e-10 -0.510555216036074 0.000257554920054182 TargetScan -0.227285941659864 2.68913187665801e-22 NA NA NA hsa-miR-200b-3p AUTS2 2.06025765178663 3.07718049337497e-10 -0.265223250836084 0.180675187907501 TargetScan -0.158666274143614 6.56372225535204e-06 NA NA NA hsa-miR-21-5p AUTS2 2.21515015440792 6.0468197386674e-49 -0.265223250836084 0.180675187907501 miRNAWalker2_validate -0.218357988341999 0.00111918037965841 NA NA NA hsa-miR-192-5p B3GALNT1 2.0867723747501 3.57854249239079e-08 0.0771986442625621 0.706882769791785 miRNAWalker2_validate -0.143362281603169 6.16045930857031e-06 NA NA NA hsa-miR-200b-3p BACH2 2.06025765178663 3.07718049337497e-10 -0.153664361619301 0.559092317470499 TargetScan -0.276072963555481 1.99960027856549e-09 NA NA NA hsa-miR-200b-3p BBX 2.06025765178663 3.07718049337497e-10 0.142954198431845 0.229679690105959 TargetScan -0.118567696955767 1.26035634239081e-08 NA NA NA hsa-miR-196a-5p BCL11A 5.05945965885472 4.48343841468539e-25 -0.932475958681757 0.00629869935051708 miRNAWalker2_validate -0.101812613354099 0.0100326639017667 NA NA NA hsa-miR-192-5p BCL2 2.0867723747501 3.57854249239079e-08 -0.899142762694703 2.93311234351261e-06 miRNAWalker2_validate -0.219076747701746 8.11784107313729e-14 26550150 MicroRNA 192 regulates chemo resistance of lung adenocarcinoma for gemcitabine and cisplatin combined therapy by targeting Bcl 2; In this paper we try to test whether miR-192 regulates chemo-resistance in human carcinoma A549 mice model by targeting Bcl-2; MTT assay real-time RT-PCR western blotting assay were used to investigate miR-192 expression levels cell viability ratio and Bcl-2 protein expression levels; Bcl-2 mRNA and protein expression levels up-regulated in miR-192 inhibitor treated tumor; Bcl-2 is a key regulator for miR-192 related chemotherapy resistance; In this study we demonstrate that miR-192 regulates chemoresistance for gemcitabine and cisplatin combined chemotherapy in human adenocarcinoma lung cancer A549 cells and Bcl-2 is the target of miR-192 drug resistance lung cancer hsa-miR-196b-5p BCL2 4.51951031652328 7.04026169031626e-18 -0.899142762694703 2.93311234351261e-06 miRNAWalker2_validate -0.135038964160811 5.10281494563971e-11 NA NA NA hsa-miR-200b-3p BCL2 2.06025765178663 3.07718049337497e-10 -0.899142762694703 2.93311234351261e-06 miRNAWalker2_validate;miRTarBase;TargetScan -0.26795392182203 1.0913241181438e-15 NA NA NA hsa-miR-21-5p BCL2 2.21515015440792 6.0468197386674e-49 -0.899142762694703 2.93311234351261e-06 miRNAWalker2_validate;miRTarBase -0.40068246416158 5.43440909882672e-10 21468550;25994220;25381586;26555418;23359184;22964582;21376256 BCL-2 up-regulation could be achieved by miR-21 overexpression which prevented T24 cells from apoptosis induced by doxorubicin; Furthermore the miR-21 induced BCL-2 up-regulation could be cancelled by the PI3K inhibitor LY294002;Meanwhile miR-21 loss reduced STAT3 and Bcl-2 activation causing an increase in the apoptosis of tumour cells in CAC mice;Changes in the sensitivity of osteosarcoma cells to CDDP were examined after transfection with miR-21 mimics or anti-miR-21 or bcl-2 siRNA in combination with CDDP;The expression of Bax Bcl-2 and miR-21 in parental and paclitaxel-resistant cells was detected by RT-PCR and Western blotting;Resveratrol induces apoptosis of pancreatic cancers cells by inhibiting miR 21 regulation of BCL 2 expression; We also used Western blot to measure BCL-2 protein levels after down-regulation of miR-21 expression; Besides down-regulation of miR-21 expression can inhibit BCL-2 expression in PANC-1 CFPAC-1 and MIA Paca-2 cells; Over-expression of miR-21 expression can reverse down-regulation of BCL-2 expression and apoptosis induced by resveratrol; In this study we demonstrated that the effect of resveratrol on apoptosis is due to inhibiting miR-21 regulation of BCL-2 expression;Tumors harvested from these lungs have elevated levels of oncogenic miRNAs miR-21 and miR-155; are deficient for p53-regulated miRNAs; and have heightened expression of miR-34 target genes such as Met and Bcl-2;Bcl 2 upregulation induced by miR 21 via a direct interaction is associated with apoptosis and chemoresistance in MIA PaCa 2 pancreatic cancer cells; However the roles and mechanisms of miRNA miR-21 in regulation of Bcl-2 in pancreatic cancer remain to be elucidated; Then luciferase activity was observed after miR-21 mimics and pRL-TK plasmids containing wild-type and mutant 3'UTRs of Bcl-2 mRNA were co-transfected; Cells transfected with miR-21 inhibitor revealed an opposite trend. There was a significant increase in luciferase activity in the cells transfected with the wild-type pRL-TK plasmid in contrast to those transfected with the mutant one indicating that miR-21 promotes Bcl-2 expression by binding directly to the 3'UTR of Bcl-2 mRNA; Upregulation of Bcl-2 directly induced by miR-21 is associated with apoptosis chemoresistance and proliferation of MIA PaCa-2 pancreatic cancer cells ;;;;;;drug resistance bladder cancer;colorectal cancer;sarcoma;breast cancer;pancreatic cancer;lung cancer;pancreatic cancer hsa-miR-133a-3p BCL2L1 -2.63342156915404 5.72661351480585e-12 0.538910243524146 0.000232226072126618 miRNAWalker2_validate -0.109066541881666 9.61762914141871e-07 23756231 Furthermore bioinformatic prediction and experimental validation were applied to identify target genes of miR-133a and the results revealed that the anti-tumor effect of miR-133a was probably due to targeting and repressing of Bcl-xL and Mcl-1 expression sarcoma hsa-miR-21-5p BCL6 2.21515015440792 6.0468197386674e-49 -0.211315139145206 0.194893611053015 miRNAWalker2_validate -0.165442720294048 0.00270909362763998 NA NA NA hsa-miR-21-5p BDH2 2.21515015440792 6.0468197386674e-49 -1.19512162130432 1.04241007480529e-13 miRNAWalker2_validate -0.275216009220252 1.05449581736892e-06 NA NA NA hsa-miR-192-5p BGLAP 2.0867723747501 3.57854249239079e-08 -0.903293918409263 8.06427733957107e-08 miRNAWalker2_validate -0.160706050932145 9.54457164408615e-10 NA NA NA hsa-miR-200b-3p BHLHE41 2.06025765178663 3.07718049337497e-10 0.353158422209973 0.127533936542101 TargetScan -0.161230519705725 9.90840208951871e-05 NA NA NA hsa-miR-200b-3p BICC1 2.06025765178663 3.07718049337497e-10 0.837229563539162 0.00140398828471014 TargetScan -0.292418835609599 2.28826204650504e-10 NA NA NA hsa-miR-200b-3p BICD2 2.06025765178663 3.07718049337497e-10 -0.0193506322306654 0.886106311165939 TargetScan -0.168752167640922 3.48350526546594e-13 NA NA NA hsa-miR-192-5p BMPR2 2.0867723747501 3.57854249239079e-08 0.205986909393828 0.0634976367094706 miRNAWalker2_validate -0.101765259373634 1.64047719638657e-09 NA NA NA hsa-miR-200b-3p BNC2 2.06025765178663 3.07718049337497e-10 -0.491277997679328 0.0998827438687705 TargetScan -0.550025928584204 2.62751551805398e-30 NA NA NA hsa-miR-21-5p BOC 2.21515015440792 6.0468197386674e-49 -0.912042259856259 0.00560150824376481 miRNAWalker2_validate -0.629147771608922 9.99087704731287e-09 NA NA NA hsa-miR-21-5p BTBD3 2.21515015440792 6.0468197386674e-49 -0.0957505542945336 0.50083712425855 miRNAWalker2_validate -0.100655440219531 0.0369619198550155 NA NA NA hsa-miR-21-5p BTBD7 2.21515015440792 6.0468197386674e-49 -0.174009462180557 0.0347294753011317 miRNAWalker2_validate -0.103503810857852 0.000177656031075862 NA NA NA hsa-miR-192-5p BTC 2.0867723747501 3.57854249239079e-08 -1.69048652094822 9.83417119475149e-10 miRNAWalker2_validate -0.255557328135743 4.99543153685461e-09 NA NA NA hsa-miR-192-5p BTF3L4 2.0867723747501 3.57854249239079e-08 0.184476147719769 0.0505801356409219 miRNAWalker2_validate -0.100898299188196 9.16070829154608e-13 NA NA NA hsa-miR-21-5p BTG2 2.21515015440792 6.0468197386674e-49 -1.7630923979864 5.46944077014254e-17 miRNAWalker2_validate;miRTarBase -0.783413431170434 7.75255849116499e-31 24821435 miR 21 regulates N methyl N nitro N' nitrosoguanidine induced gastric tumorigenesis by targeting FASLG and BTG2 tumorigenesis gastric cancer hsa-miR-18a-5p BVES 2.04701000113742 1.30593450319289e-12 -1.51355443880608 7.967744286549e-06 miRNAWalker2_validate -0.688278393434613 8.95261759656455e-28 NA NA NA hsa-miR-192-5p C1QTNF3 2.0867723747501 3.57854249239079e-08 -1.2857015633976 2.75834869264902e-05 miRNAWalker2_validate -0.375602276827499 4.31975926078151e-16 NA NA NA hsa-miR-192-5p CAB39L 2.0867723747501 3.57854249239079e-08 -1.76562312798893 2.94345232906232e-14 miRNAWalker2_validate -0.397869047418757 1.92670095418945e-31 NA NA NA hsa-miR-200b-3p CACHD1 2.06025765178663 3.07718049337497e-10 -0.320745871977507 0.138986353847716 TargetScan -0.135520209219911 0.000484461288319484 NA NA NA hsa-miR-200b-3p CACNA1C 2.06025765178663 3.07718049337497e-10 -0.182409901963044 0.557004423318687 TargetScan -0.534302005247156 7.19545908944889e-26 NA NA NA hsa-miR-200b-3p CACNA2D1 2.06025765178663 3.07718049337497e-10 -0.846445244760543 0.00675290229856498 TargetScan -0.42715161103858 1.77599163924932e-15 NA NA NA hsa-miR-192-5p CADM1 2.0867723747501 3.57854249239079e-08 -0.899936241890849 0.000844138987328649 miRNAWalker2_validate -0.255287068605976 7.16867093595076e-10 NA NA NA hsa-miR-200b-3p CADM1 2.06025765178663 3.07718049337497e-10 -0.899936241890849 0.000844138987328649 TargetScan -0.262479616962518 4.3464640649513e-08 NA NA NA hsa-miR-21-5p CALD1 2.21515015440792 6.0468197386674e-49 -0.498524803482272 0.0854448127355164 miRNAWalker2_validate -0.474696767410786 9.91339917127746e-07 NA NA NA hsa-miR-192-5p CAMK4 2.0867723747501 3.57854249239079e-08 -0.596232499132412 0.011832094031569 miRNAWalker2_validate -0.100142787955831 0.00723122161813675 NA NA NA hsa-miR-200b-3p CAMSAP2 2.06025765178663 3.07718049337497e-10 0.423832054082736 0.000532909035924399 TargetScan -0.116070704028349 9.08905666331661e-08 NA NA NA hsa-miR-1-3p CAND1 -2.73171785118192 3.4424534921136e-12 0.404447093370241 0.000129508898109157 miRTarBase -0.110520366094798 3.07016260270459e-13 NA NA NA hsa-miR-200b-3p CASD1 2.06025765178663 3.07718049337497e-10 -0.0564635591512928 0.674318190720727 TargetScan -0.144246407370863 7.01726340330353e-10 NA NA NA hsa-miR-200b-3p CBX5 2.06025765178663 3.07718049337497e-10 0.164944902002406 0.244455396933291 TargetScan -0.104982650310067 3.08865043207079e-05 NA NA NA hsa-miR-21-5p CCNG1 2.21515015440792 6.0468197386674e-49 -0.702987782947956 5.39693567551696e-07 miRNAWalker2_validate -0.160860427066878 0.000886365792680893 NA NA NA hsa-miR-200b-3p CDC42EP3 2.06025765178663 3.07718049337497e-10 -0.123901712398209 0.566980171516008 TargetScan -0.372934006694852 5.07814006443935e-26 NA NA NA hsa-miR-200b-3p CDH11 2.06025765178663 3.07718049337497e-10 1.42671930314161 2.20201589928761e-10 TargetScan -0.2017247638054 1.02741801913574e-06 NA NA NA hsa-miR-194-5p CDH2 2.24386881692218 9.35116329002209e-11 -0.676171143790671 0.0404221186379083 miRNAWalker2_validate;miRTarBase -0.382377018680577 1.30645514844207e-12 21845495;20979124 The results of real-time PCR and western blot highlighted that miR-194 interacted with N-cadherin and negatively regulated its expression at the translational level;The overexpression of miR-194 in liver mesenchymal-like cancer cells reduced the expression of the mesenchymal cell marker N-cadherin and suppressed invasion and migration of the mesenchymal-like cancer cells both in vitro and in vivo ; gastric cancer;liver cancer hsa-miR-192-5p CDK14 2.0867723747501 3.57854249239079e-08 -0.300429156166662 0.186723710515312 miRNAWalker2_validate -0.238436217082729 3.67128683954332e-12 NA NA NA hsa-miR-200b-3p CDK17 2.06025765178663 3.07718049337497e-10 0.0694540025336501 0.487075282749813 TargetScan -0.122045467525224 1.10168728267942e-12 NA NA NA hsa-miR-146b-5p CDKN1A 2.23105707690238 3.83997814011313e-23 -1.26866697234787 1.6534116759688e-12 miRNAWalker2_validate -0.215180106045082 3.12241828598735e-06 27602131 During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis progression thyroid cancer hsa-miR-192-5p CDON 2.0867723747501 3.57854249239079e-08 -0.742711204088021 0.0145188883462419 miRNAWalker2_validate -0.414139414072637 1.17943038016748e-20 NA NA NA hsa-miR-200b-3p CEBPD 2.06025765178663 3.07718049337497e-10 -0.985425400909972 5.1357147416495e-09 TargetScan -0.224360051416899 5.61546314394064e-14 NA NA NA hsa-miR-200b-3p CECR2 2.06025765178663 3.07718049337497e-10 -0.991511187827841 0.00378647640083668 TargetScan -0.378890240582351 3.0995711555874e-10 NA NA NA hsa-miR-200b-3p CELF2 2.06025765178663 3.07718049337497e-10 -1.10167045974223 7.93604111646927e-06 TargetScan -0.431502230250276 8.39291923663402e-26 NA NA NA hsa-miR-200b-3p CEP85L 2.06025765178663 3.07718049337497e-10 -0.892076780989505 7.35720788146592e-07 TargetScan -0.191512590990988 2.82593021177595e-09 NA NA NA hsa-miR-192-5p CFL2 2.0867723747501 3.57854249239079e-08 -1.29748949709607 2.10137209702745e-06 miRNAWalker2_validate -0.480403942492533 2.21554593265399e-36 NA NA NA hsa-miR-200b-3p CFL2 2.06025765178663 3.07718049337497e-10 -1.29748949709607 2.10137209702745e-06 TargetScan -0.611661644743514 1.78443301504938e-48 NA NA NA hsa-miR-192-5p CGNL1 2.0867723747501 3.57854249239079e-08 -1.61265143988687 5.1888718612151e-07 miRNAWalker2_validate -0.435362816435892 1.30646694372153e-19 NA NA NA hsa-miR-200b-3p CHRDL1 2.06025765178663 3.07718049337497e-10 -2.81435068736559 3.17200324047588e-06 TargetScan -1.01209922554632 3.13218780857773e-23 NA NA NA hsa-miR-200b-3p CHST9 2.06025765178663 3.07718049337497e-10 -3.19932529158582 1.98123629158033e-07 TargetScan -0.578561259418833 2.05978900486222e-07 NA NA NA hsa-miR-200b-3p CITED2 2.06025765178663 3.07718049337497e-10 -1.54489997373465 2.4800656042848e-22 TargetScan -0.292403787339343 7.6894781536567e-25 NA NA NA hsa-miR-196b-5p CKB 4.51951031652328 7.04026169031626e-18 -2.84100469375822 1.80384318532105e-15 miRNAWalker2_validate -0.228223759110231 1.28387972057116e-08 NA NA NA hsa-miR-935 CKB 2.321600496365 1.00042201726114e-07 -2.84100469375822 1.80384318532105e-15 miRNAWalker2_validate -0.407137028249589 4.02746473570552e-15 NA NA NA hsa-miR-200b-3p CLASP1 2.06025765178663 3.07718049337497e-10 0.0864210099182321 0.350450750205728 TargetScan -0.135482032934453 2.30143104436211e-18 NA NA NA hsa-miR-200b-3p CLIC4 2.06025765178663 3.07718049337497e-10 -0.38961849989088 0.0412415922050508 TargetScan -0.372500384903988 5.2494412504583e-35 NA NA NA hsa-miR-200b-3p CLIP1 2.06025765178663 3.07718049337497e-10 -0.215282583297315 0.0868385355728641 TargetScan -0.196402081196344 7.21594843596929e-21 NA NA NA hsa-miR-192-5p CLIP4 2.0867723747501 3.57854249239079e-08 -1.15401000711272 6.33323986127773e-06 miRNAWalker2_validate -0.422031813886014 2.91864723770517e-31 NA NA NA hsa-miR-21-5p CLIP4 2.21515015440792 6.0468197386674e-49 -1.15401000711272 6.33323986127773e-06 miRNAWalker2_validate -0.520128166573803 1.40974934662611e-09 NA NA NA hsa-miR-192-5p CLK1 2.0867723747501 3.57854249239079e-08 -0.158615842799502 0.223068275923641 miRNAWalker2_validate -0.116810960574069 3.75978286455058e-09 NA NA NA hsa-miR-192-5p CLK4 2.0867723747501 3.57854249239079e-08 -0.0818303339579662 0.491847906638591 miRNAWalker2_validate -0.108598349447499 1.84257392433686e-09 NA NA NA hsa-miR-200b-3p CNN3 2.06025765178663 3.07718049337497e-10 -0.562615101587786 0.000315898705221665 TargetScan -0.23661988107496 4.65737922466567e-19 NA NA NA hsa-miR-200b-3p CNTFR 2.06025765178663 3.07718049337497e-10 -3.85530026542554 4.24189598216231e-12 TargetScan -0.486805626422576 2.38127269344753e-06 NA NA NA hsa-miR-200b-3p CNTN4 2.06025765178663 3.07718049337497e-10 -0.828286345837398 0.00133313844224883 TargetScan -0.277582421078064 1.09690256226905e-09 NA NA NA hsa-miR-192-5p CNTNAP3 2.0867723747501 3.57854249239079e-08 -1.46487148437228 0.000329660247305951 miRNAWalker2_validate -0.526478118772523 4.24037687642614e-18 NA NA NA hsa-miR-133a-3p COL1A1 -2.63342156915404 5.72661351480585e-12 3.01832941044284 9.17776951829232e-25 miRNAWalker2_validate -0.147252420806544 0.00287134050361538 NA NA NA hsa-miR-200b-3p COL4A3 2.06025765178663 3.07718049337497e-10 -0.748410740217012 0.0361489474679936 TargetScan -0.329085070334415 2.00627160069928e-07 NA NA NA hsa-miR-200b-3p CORO1C 2.06025765178663 3.07718049337497e-10 -0.0594383479650764 0.605555284745698 TargetScan -0.204804646220886 2.4463057662874e-28 NA NA NA hsa-miR-192-5p CORO2B 2.0867723747501 3.57854249239079e-08 -1.17741661882517 2.06880794851619e-05 miRNAWalker2_validate -0.317894527561999 3.88105880453811e-14 NA NA NA hsa-miR-21-5p CPEB3 2.21515015440792 6.0468197386674e-49 -0.726462801367756 6.97488351116509e-10 miRNAWalker2_validate -0.216267807811555 8.62942699277487e-08 NA NA NA hsa-miR-183-5p CR1 2.08412478942637 1.08931431254487e-10 -0.137331836906051 0.656366490278349 miRNAWalker2_validate -0.271636457682277 8.85870544308646e-07 NA NA NA hsa-miR-200b-3p CREB5 2.06025765178663 3.07718049337497e-10 0.0144734137836222 0.953004773309416 TargetScan -0.277717096886131 7.85164672894072e-11 NA NA NA hsa-miR-18a-5p CREBL2 2.04701000113742 1.30593450319289e-12 -0.289272671162855 0.00289044434753105 miRNAWalker2_validate -0.154826000212788 7.07074932603383e-17 NA NA NA hsa-miR-200b-3p CRTAP 2.06025765178663 3.07718049337497e-10 -0.491357908154423 0.000138058915693051 TargetScan -0.253353921703587 5.66438924935477e-35 NA NA NA hsa-miR-192-5p CSGALNACT2 2.0867723747501 3.57854249239079e-08 0.609558649145237 2.24794845816362e-07 miRNAWalker2_validate -0.112413364526062 7.88576819703072e-10 NA NA NA hsa-miR-18a-5p CSRNP3 2.04701000113742 1.30593450319289e-12 -0.890893120904682 0.000731559220338997 miRNAWalker2_validate -0.395610954750672 1.27569727021297e-14 NA NA NA hsa-miR-200b-3p CSRNP3 2.06025765178663 3.07718049337497e-10 -0.890893120904682 0.000731559220338997 TargetScan -0.334074441789312 3.44252384732335e-13 NA NA NA hsa-miR-18a-5p CTGF 2.04701000113742 1.30593450319289e-12 0.320520687991875 0.116821048397881 miRNAWalker2_validate;miRTarBase -0.229318859680867 1.38859698228017e-08 23249750 Targeting of TGFβ signature and its essential component CTGF by miR 18 correlates with improved survival in glioblastoma; Indeed microRNA-18a but not other miR-17∼92 members has a functional site in the CTGF 3' UTR and its forced reexpression sharply reduces CTGF protein and mRNA levels; The unexpected effects of miR-18a on CTGF transcription are mediated in part by direct targeting of Smad3 and ensuing weakening of TGFβ signaling poor survival glioblastoma hsa-miR-200b-3p CTNND2 2.06025765178663 3.07718049337497e-10 -1.4845827521637 0.00260429491755341 TargetScan -0.352972532134604 6.92958623666048e-05 NA NA NA hsa-miR-21-5p CYBRD1 2.21515015440792 6.0468197386674e-49 -1.57319886365625 1.13822187395473e-09 miRNAWalker2_validate -0.541525880464131 7.95535005726743e-10 NA NA NA hsa-miR-200b-3p CYP1B1 2.06025765178663 3.07718049337497e-10 -0.767683097481093 0.0530672981235295 TargetScan -0.625729296071077 4.50817949833391e-21 NA NA NA hsa-miR-200b-3p CYTH3 2.06025765178663 3.07718049337497e-10 0.117630101294158 0.359244933780906 TargetScan -0.143720007633974 1.09326673889832e-10 NA NA NA hsa-miR-21-5p DAAM1 2.21515015440792 6.0468197386674e-49 -0.338417179657082 0.0105035166930411 miRNAWalker2_validate -0.191165521420723 1.74085280692908e-05 NA NA NA hsa-miR-18a-5p DAAM2 2.04701000113742 1.30593450319289e-12 -1.34031580644644 1.14961034820812e-06 miRNAWalker2_validate -0.593477403081751 8.46467621317108e-32 NA NA NA hsa-miR-200b-3p DACT1 2.06025765178663 3.07718049337497e-10 0.783084312335694 0.00072482171739131 TargetScan -0.19615420673442 2.25778789045408e-06 NA NA NA hsa-miR-200b-3p DCAF5 2.06025765178663 3.07718049337497e-10 -0.432022552798976 2.11919388779901e-08 TargetScan -0.117968047733686 4.16585681627222e-19 NA NA NA hsa-miR-21-5p DCAF8 2.21515015440792 6.0468197386674e-49 -0.360410760328999 1.01481307378452e-08 miRNAWalker2_validate -0.196533339601291 5.67891964405955e-23 NA NA NA hsa-miR-192-5p DCBLD2 2.0867723747501 3.57854249239079e-08 -0.142686813220974 0.552400988273795 miRNAWalker2_validate -0.266087004125423 1.3538705034276e-13 NA NA NA hsa-miR-200b-3p DCBLD2 2.06025765178663 3.07718049337497e-10 -0.142686813220974 0.552400988273795 TargetScan -0.314823423852922 1.99333147051828e-14 NA NA NA hsa-miR-21-5p DDHD2 2.21515015440792 6.0468197386674e-49 -0.154583103342212 0.193842372532219 miRNAWalker2_validate -0.116144211349697 0.00385437164328784 NA NA NA hsa-miR-21-5p DDR2 2.21515015440792 6.0468197386674e-49 -0.761379109868812 0.0066880292071355 miRNAWalker2_validate -0.495161070948485 1.38635305253879e-07 NA NA NA hsa-miR-200b-3p DDX3Y 2.06025765178663 3.07718049337497e-10 -1.08011892315372 0.269270157228425 TargetScan -0.378958463767422 0.0318118681059195 NA NA NA hsa-miR-192-5p DEGS1 2.0867723747501 3.57854249239079e-08 -0.172879256073472 0.103647058225951 miRNAWalker2_validate -0.105462847337841 4.68525392348291e-11 NA NA NA hsa-miR-200b-3p DENND5A 2.06025765178663 3.07718049337497e-10 -0.152606794957467 0.390594828957252 TargetScan -0.335479849422894 1.89571003610997e-32 NA NA NA hsa-miR-200b-3p DIRAS2 2.06025765178663 3.07718049337497e-10 -2.05223261985053 1.29402751264844e-05 TargetScan -0.173060093145271 0.0452325597936238 NA NA NA hsa-miR-200b-3p DIXDC1 2.06025765178663 3.07718049337497e-10 -1.16493051519743 1.82036516809915e-06 TargetScan -0.485006218246082 5.46445881627915e-35 NA NA NA hsa-miR-200b-3p DLC1 2.06025765178663 3.07718049337497e-10 -0.381837238292576 0.0503828744141603 TargetScan -0.322209760284385 1.99020324412842e-23 NA NA NA hsa-miR-192-5p DLG5 2.0867723747501 3.57854249239079e-08 0.258176717985961 0.0632228839548884 miRNAWalker2_validate -0.128858531237489 1.13363732934286e-09 NA NA NA hsa-miR-200a-3p DLX5 2.02394729844446 2.21240501925011e-08 0.467035033598791 0.229765245212111 miRNAWalker2_validate;miRTarBase -0.268013516383989 2.19054842959425e-05 NA NA NA hsa-miR-200b-3p DMD 2.06025765178663 3.07718049337497e-10 -1.50584693629303 4.05157194167937e-06 TargetScan -0.601552511353115 5.33364821089218e-29 NA NA NA hsa-miR-21-5p DMD 2.21515015440792 6.0468197386674e-49 -1.50584693629303 4.05157194167937e-06 miRNAWalker2_validate -0.805216804768577 9.17890581926293e-14 NA NA NA hsa-miR-192-5p DNAJB4 2.0867723747501 3.57854249239079e-08 -0.699790825105696 7.61829706903065e-06 miRNAWalker2_validate -0.258570415571564 1.51422887158205e-31 NA NA NA hsa-miR-192-5p DNAJB5 2.0867723747501 3.57854249239079e-08 -1.63239432731099 4.88248701689133e-08 miRNAWalker2_validate -0.536416141443651 1.03386568429183e-37 NA NA NA hsa-miR-200b-3p DNAJB5 2.06025765178663 3.07718049337497e-10 -1.63239432731099 4.88248701689133e-08 TargetScan -0.684464035071018 7.69388306734523e-51 NA NA NA hsa-miR-200b-3p DNAJC18 2.06025765178663 3.07718049337497e-10 -0.991567847547574 6.93843475640889e-08 TargetScan -0.392810907369169 6.6029430388378e-42 NA NA NA hsa-miR-192-5p DNAL1 2.0867723747501 3.57854249239079e-08 0.0480634598292551 0.634935736519382 miRNAWalker2_validate -0.118779383699473 1.95674694853594e-15 NA NA NA hsa-miR-200b-3p DNMT3A 2.06025765178663 3.07718049337497e-10 0.301869013127361 0.0262018308733428 TargetScan -0.106005375987799 1.15742159683197e-05 NA NA NA hsa-miR-200b-3p DOCK4 2.06025765178663 3.07718049337497e-10 0.502120813551762 0.00108492735386023 TargetScan -0.122601879526226 8.34236139890405e-06 NA NA NA hsa-miR-21-5p DSE 2.21515015440792 6.0468197386674e-49 -0.231318207076859 0.0534669114878373 miRNAWalker2_validate -0.108614468692268 0.00745491647752206 NA NA NA hsa-miR-192-5p DST 2.0867723747501 3.57854249239079e-08 -0.712661407535561 0.000959062290630929 miRNAWalker2_validate -0.267602801247337 9.52253045322956e-17 NA NA NA hsa-miR-192-5p DSTYK 2.0867723747501 3.57854249239079e-08 0.140649893281743 0.203401353379972 miRNAWalker2_validate -0.151260304560656 3.01849739774487e-21 NA NA NA hsa-miR-200b-3p DTNA 2.06025765178663 3.07718049337497e-10 -1.64790334674543 7.31937738648404e-06 TargetScan -0.673152027501618 1.03884336718138e-28 NA NA NA hsa-miR-200b-3p DUSP1 2.06025765178663 3.07718049337497e-10 -1.75402429649966 1.13529116708344e-13 TargetScan -0.341211822872468 7.80535205483735e-16 NA NA NA hsa-miR-21-5p DUSP8 2.21515015440792 6.0468197386674e-49 -0.138770521214301 0.439264281546499 miRNAWalker2_validate -0.183282023719592 0.00252174568625556 NA NA NA hsa-miR-21-5p DYNC1LI2 2.21515015440792 6.0468197386674e-49 -0.151678434580852 0.11155732630237 miRNAWalker2_validate -0.107993496007619 0.000751077287290826 NA NA NA hsa-miR-200b-3p DZIP1 2.06025765178663 3.07718049337497e-10 -0.88362320879615 0.00028568683997458 TargetScan -0.459367082783166 2.23483215657587e-31 NA NA NA hsa-miR-21-5p ECI2 2.21515015440792 6.0468197386674e-49 -0.874473345533636 1.26576116281351e-08 miRNAWalker2_validate -0.243625122163077 4.10547171377639e-06 NA NA NA hsa-miR-21-5p EDIL3 2.21515015440792 6.0468197386674e-49 -0.975272918471383 5.22685481386266e-06 miRNAWalker2_validate -0.369963387882668 3.56890675405255e-07 NA NA NA hsa-miR-192-5p EEF1A2 2.0867723747501 3.57854249239079e-08 -3.58448086492029 3.42501683188058e-10 miRNAWalker2_validate -0.380332471366233 3.50156845837547e-05 NA NA NA hsa-miR-200b-3p EFNA5 2.06025765178663 3.07718049337497e-10 -0.421335116813535 0.178679228276983 TargetScan -0.206540537480006 0.000230717411847866 NA NA NA hsa-miR-183-5p EGR1 2.08412478942637 1.08931431254487e-10 -1.19548185353943 2.58321740617567e-06 miRNAWalker2_validate;miRTarBase -0.265319921884661 8.44246816462655e-09 NA NA NA hsa-miR-192-5p EGR1 2.0867723747501 3.57854249239079e-08 -1.19548185353943 2.58321740617567e-06 miRNAWalker2_validate -0.188229355580979 2.61936868526192e-06 NA NA NA hsa-miR-200b-3p EGR3 2.06025765178663 3.07718049337497e-10 -0.979948930483222 0.000185818581104659 TargetScan -0.280465895520504 1.56313188426172e-09 NA NA NA hsa-miR-192-5p EHBP1L1 2.0867723747501 3.57854249239079e-08 -0.377076908313878 0.00297956695224553 miRNAWalker2_validate -0.141231904284581 1.43584840470881e-13 NA NA NA hsa-miR-21-5p EIF4A2 2.21515015440792 6.0468197386674e-49 -0.382805867476675 0.000458178068034746 miRNAWalker2_validate;miRTarBase -0.117279159716951 0.00161150274188387 NA NA NA hsa-miR-200b-3p EIF4E3 2.06025765178663 3.07718049337497e-10 -1.24277770218871 4.31281769728062e-12 TargetScan -0.2451038959554 2.32931002112101e-14 NA NA NA hsa-miR-21-5p EIF4EBP2 2.21515015440792 6.0468197386674e-49 -0.315823877158708 0.000627369821832842 miRNAWalker2_validate -0.14295797939137 4.03805649840889e-06 NA NA NA hsa-miR-200b-3p ELAVL4 2.06025765178663 3.07718049337497e-10 -1.05201490861251 0.00375987986315775 TargetScan -0.488517170097894 5.94251871262108e-15 NA NA NA hsa-miR-21-5p ELAVL4 2.21515015440792 6.0468197386674e-49 -1.05201490861251 0.00375987986315775 miRNAWalker2_validate -0.597052178130805 1.02552347405006e-06 NA NA NA hsa-miR-200b-3p ELK3 2.06025765178663 3.07718049337497e-10 0.186740798453513 0.188175135041682 TargetScan -0.127862338479325 3.25323092584741e-07 NA NA NA hsa-miR-192-5p ELL2 2.0867723747501 3.57854249239079e-08 -1.46362215725712 1.58717825670906e-20 miRNAWalker2_validate -0.196449840616178 9.97331759997534e-15 NA NA NA hsa-miR-200b-3p ELL2 2.06025765178663 3.07718049337497e-10 -1.46362215725712 1.58717825670906e-20 TargetScan -0.20684374064257 2.10091962941171e-12 NA NA NA hsa-miR-21-5p ELOVL4 2.21515015440792 6.0468197386674e-49 -1.19952854478656 1.99479557964071e-05 miRNAWalker2_validate -0.608559626101489 9.014665489221e-11 NA NA NA hsa-miR-192-5p ELOVL5 2.0867723747501 3.57854249239079e-08 -0.0910045021719972 0.633667869483248 miRNAWalker2_validate -0.209795049625131 2.16570286132685e-13 NA NA NA hsa-miR-192-5p EML1 2.0867723747501 3.57854249239079e-08 -1.03848400994716 8.54789799498598e-05 miRNAWalker2_validate -0.391321229609126 2.24377940678663e-24 NA NA NA hsa-miR-21-5p ENAH 2.21515015440792 6.0468197386674e-49 -0.303508505660234 0.0898566571919018 miRNAWalker2_validate -0.279307247856932 3.14847372848835e-06 NA NA NA hsa-miR-192-5p ENDOD1 2.0867723747501 3.57854249239079e-08 -0.311613833349303 0.0226809514564935 miRNAWalker2_validate -0.101360061412654 1.60841216302576e-06 NA NA NA hsa-miR-192-5p ENTPD3 2.0867723747501 3.57854249239079e-08 -2.57455302613472 2.46343986336398e-11 miRNAWalker2_validate -0.2857339057772 4.24381139285329e-06 NA NA NA hsa-miR-192-5p EPDR1 2.0867723747501 3.57854249239079e-08 -0.267558637525019 0.269780074713144 miRNAWalker2_validate -0.112662647482773 0.00296853631235063 NA NA NA hsa-miR-21-5p EPHA4 2.21515015440792 6.0468197386674e-49 -0.721045772095589 0.00956988810687138 miRNAWalker2_validate -0.416201980009361 9.19477583509674e-06 NA NA NA hsa-miR-196a-5p EPHA7 5.05945965885472 4.48343841468539e-25 -2.19739166323181 3.30440512414909e-05 miRNAWalker2_validate -0.39939634241845 2.29377801567576e-11 NA NA NA hsa-miR-192-5p EPM2A 2.0867723747501 3.57854249239079e-08 -1.00729583125 1.98879330342911e-07 miRNAWalker2_validate -0.339687396633476 6.28341444958103e-36 NA NA NA hsa-miR-21-5p EPM2A 2.21515015440792 6.0468197386674e-49 -1.00729583125 1.98879330342911e-07 miRNAWalker2_validate -0.598076009274688 6.95318033824996e-22 NA NA NA hsa-miR-192-5p EPM2AIP1 2.0867723747501 3.57854249239079e-08 -0.374479682925325 0.0526272510372513 miRNAWalker2_validate -0.221592516986378 1.74137463631655e-14 NA NA NA hsa-miR-200b-3p ERBB4 2.06025765178663 3.07718049337497e-10 -1.97496082958559 1.58728422015894e-05 TargetScan -0.312066132439956 0.000176442888096672 NA NA NA hsa-miR-183-5p ERC1 2.08412478942637 1.08931431254487e-10 -0.107831770859248 0.387937444516273 miRNAWalker2_validate -0.161563273167404 8.35134459272146e-14 NA NA NA hsa-miR-200b-3p ERC1 2.06025765178663 3.07718049337497e-10 -0.107831770859248 0.387937444516273 TargetScan -0.200523473595961 2.81187064500887e-22 NA NA NA hsa-miR-200b-3p ERG 2.06025765178663 3.07718049337497e-10 0.00212829554190064 0.990109995094702 TargetScan -0.222000071451755 4.46970236013955e-14 27191272 The tumor suppressive miR 200b subfamily is an ERG target gene in human prostate tumors; Although induction of the tumor suppressive miR-200b subfamily by oncogenic ERG appears to be counterintuitive it is consistent with the observation that the vast majority of primary prostate cancers are slow-growing and indolent prostate cancer hsa-miR-18a-5p ERRFI1 2.04701000113742 1.30593450319289e-12 -0.797991095855851 8.16500395108496e-06 miRNAWalker2_validate -0.222270710965566 4.889617965357e-10 NA NA NA hsa-miR-200b-3p ERRFI1 2.06025765178663 3.07718049337497e-10 -0.797991095855851 8.16500395108496e-06 TargetScan -0.255031617869948 1.61384134408975e-16 NA NA NA hsa-miR-18a-5p ESR1 2.04701000113742 1.30593450319289e-12 -1.03540584472649 2.65202027022478e-05 miRNAWalker2_validate;miRTarBase -0.428841610453799 1.07620378964708e-19 19027010;24975878 MicroRNA 18a prevents estrogen receptor alpha expression promoting proliferation of hepatocellular carcinoma cells; The gene ESR1 which encodes the estrogen receptor-alpha ERalpha was identified as a target of miR-18a miR-18a can repress ERalpha translation by binding to its mRNA at the 3' untranslated region;Elevated p53 promotes the processing of miR 18a to decrease estrogen receptor α in female hepatocellular carcinoma; Our previous study identified that estrogen receptor alpha ERα protein is downregulated in 60% of female HCC cases via a miR-18a elevation mediated suppression of ERα translation ; liver cancer;liver cancer hsa-miR-200b-3p ESRRG 2.06025765178663 3.07718049337497e-10 -4.81883837531082 5.12622713493389e-21 TargetScan -0.310232569677695 0.0018627473269641 NA NA NA hsa-miR-192-5p EXTL2 2.0867723747501 3.57854249239079e-08 0.109243729813074 0.373750128185246 miRNAWalker2_validate -0.131604424129497 8.32060354897344e-13 NA NA NA hsa-miR-192-5p FADS1 2.0867723747501 3.57854249239079e-08 0.270403268873646 0.21340410024689 miRNAWalker2_validate -0.135471574739071 5.99581640159027e-05 NA NA NA hsa-miR-183-5p FAM110B 2.08412478942637 1.08931431254487e-10 -1.10453149128806 1.21017749000064e-05 miRNAWalker2_validate -0.349349194127216 3.64917454821309e-15 NA NA NA hsa-miR-18a-5p FAM120C 2.04701000113742 1.30593450319289e-12 0.170776103390755 0.263203241775743 miRNAWalker2_validate -0.126024114428764 3.6717799385452e-05 NA NA NA hsa-miR-192-5p FAM126A 2.0867723747501 3.57854249239079e-08 -0.0664935237033619 0.72412799652184 miRNAWalker2_validate -0.105399370799883 0.000321347632662215 NA NA NA hsa-miR-200b-3p FAM126A 2.06025765178663 3.07718049337497e-10 -0.0664935237033619 0.72412799652184 TargetScan -0.136391539391253 4.75362747304217e-05 NA NA NA hsa-miR-196a-5p FAM127A 5.05945965885472 4.48343841468539e-25 -0.726381248486746 0.000138289728116872 miRNAWalker2_validate -0.123512472441336 1.18778620051509e-08 NA NA NA hsa-miR-18a-5p FAM127B 2.04701000113742 1.30593450319289e-12 -0.561735132927407 0.00941023505557202 miRNAWalker2_validate -0.150231487519424 0.000601013708238945 NA NA NA hsa-miR-200b-3p FAM168B 2.06025765178663 3.07718049337497e-10 -0.135313494128928 0.193723824290666 TargetScan -0.110399625337369 1.11375152311079e-09 NA NA NA hsa-miR-192-5p FAM171B 2.0867723747501 3.57854249239079e-08 -0.0176591718289498 0.927528339765729 miRNAWalker2_validate -0.188557307480295 1.43845162990559e-10 NA NA NA hsa-miR-200b-3p FAM179B 2.06025765178663 3.07718049337497e-10 -0.167488194439022 0.19857804382265 TargetScan -0.11893603875181 2.18362927380513e-07 NA NA NA hsa-miR-192-5p FAM189A2 2.0867723747501 3.57854249239079e-08 -2.26859785003332 8.09998388383058e-08 miRNAWalker2_validate -0.229625668198354 0.000701137734357473 NA NA NA hsa-miR-200b-3p FAM19A5 2.06025765178663 3.07718049337497e-10 1.69117921336791 2.05488338044706e-09 TargetScan -0.190811529691732 0.000253191322318827 NA NA NA hsa-miR-192-5p FAM20B 2.0867723747501 3.57854249239079e-08 0.0115906902718228 0.915994905003775 miRNAWalker2_validate -0.119288009804203 3.46163894348874e-13 NA NA NA hsa-miR-21-5p FAM20B 2.21515015440792 6.0468197386674e-49 0.0115906902718228 0.915994905003775 miRNAWalker2_validate -0.122706915846483 0.000892710911420458 NA NA NA hsa-miR-200b-3p FAM219A 2.06025765178663 3.07718049337497e-10 0.218628262317111 0.0484705408275253 TargetScan -0.118620189848456 8.43793659422667e-10 NA NA NA hsa-miR-21-5p FAM46A 2.21515015440792 6.0468197386674e-49 -0.731253149491049 4.51804141138159e-05 miRNAWalker2_validate -0.192775115940049 0.0017226388588753 NA NA NA hsa-miR-192-5p FAM63B 2.0867723747501 3.57854249239079e-08 0.0653334389343767 0.616352094767286 miRNAWalker2_validate -0.166127913589892 3.31128427701621e-18 NA NA NA hsa-miR-200b-3p FAM63B 2.06025765178663 3.07718049337497e-10 0.0653334389343767 0.616352094767286 TargetScan -0.122069457785558 9.85615708532093e-08 NA NA NA hsa-miR-192-5p FAM83D 2.0867723747501 3.57854249239079e-08 0.190720348515486 0.389470030829468 miRNAWalker2_validate -0.256050219801128 8.91661096170149e-15 NA NA NA hsa-miR-200b-3p FAM8A1 2.06025765178663 3.07718049337497e-10 -0.222229045123044 0.0265174303093394 TargetScan -0.107500577717425 7.52592007226654e-10 NA NA NA hsa-miR-200b-3p FARP1 2.06025765178663 3.07718049337497e-10 0.504657815201534 0.00187642310069963 TargetScan -0.101546671329369 0.000518669775416538 NA NA NA hsa-miR-183-5p FAT2 2.08412478942637 1.08931431254487e-10 -0.278356365218703 0.44877423623627 miRNAWalker2_validate -0.154197493953449 0.0213965039338374 NA NA NA hsa-miR-200b-3p FAT3 2.06025765178663 3.07718049337497e-10 -1.60082132479507 0.000514379028219012 TargetScan -0.881557042785804 1.65832699347662e-32 NA NA NA hsa-miR-192-5p FAXC 2.0867723747501 3.57854249239079e-08 -1.51914231075936 0.000645899358679245 miRNAWalker2_validate -0.656924778648182 2.39711296148564e-24 NA NA NA hsa-miR-192-5p FBN1 2.0867723747501 3.57854249239079e-08 0.691519482636648 0.00278726958162726 miRNAWalker2_validate -0.179668638948456 5.50319382495199e-07 NA NA NA hsa-miR-21-5p FBXL17 2.21515015440792 6.0468197386674e-49 -0.585994253234401 1.52454989342516e-08 miRNAWalker2_validate -0.164003877266903 3.85205643120302e-06 NA NA NA hsa-miR-21-5p FBXL2 2.21515015440792 6.0468197386674e-49 -0.0416322263648743 0.858599282744402 miRNAWalker2_validate -0.198573361438143 0.0122423354122405 NA NA NA hsa-miR-21-5p FBXO3 2.21515015440792 6.0468197386674e-49 -0.554476776198341 4.67339274952818e-11 miRNAWalker2_validate -0.194996796654829 6.71063553628614e-12 NA NA NA hsa-miR-200b-3p FBXO30 2.06025765178663 3.07718049337497e-10 -0.260322470127411 0.0645263272243245 TargetScan -0.23657851130304 1.73939025836622e-24 NA NA NA hsa-miR-200b-3p FERMT2 2.06025765178663 3.07718049337497e-10 -0.896574191403877 0.000646206347161597 TargetScan -0.567914531506366 3.43180257889084e-45 23483548;24064224;26334393 Here we report that Kindlin 2 markedly downregulates the expression of miR-200 family by inducing CpG island hypermethylation; Our data indicate that Kindlin 2 plays a novel role in epigenetic repression of miR-200 family a mechanism that promotes breast cancer invasion;miR 200b suppresses invasiveness and modulates the cytoskeletal and adhesive machinery in esophageal squamous cell carcinoma cells via targeting Kindlin 2; Quantitative mass spectrometry identified 57 putative miR-200b targets including Kindlin-2 previously implicated in the regulation of tumor invasiveness and actin cytoskeleton in other cell types; Enforced expression of miR-200b mimic in ESCC cells led to a decrease of Kindlin-2 expression whereas transfection of miR-200b inhibitor induced Kindlin-2 expression; Furthermore transfection of miR-200b mimic or knockdown of Kindlin-2 in ESCC cells decreased cell protrusion and focal adhesion FA formation reduced cell spreading and invasiveness/migration; Enforced expression of Kindlin-2 largely abrogated the inhibitory effects of miR-200b on ESCC cell invasiveness; The tumor suppressor effects of miR-200b may be due to its suppression of Kindlin-2 a novel target of miR-200b that modulates actin cytoskeleton FA formation and the migratory/invasiveness properties of ESCC;We revealed that miR-200b suppresses the integrin β1-AKT pathway via targeting Kindlin-2 to mitigate ESCC cell invasiveness ;; breast cancer;esophageal cancer;esophageal cancer hsa-miR-21-5p FERMT2 2.21515015440792 6.0468197386674e-49 -0.896574191403877 0.000646206347161597 miRNAWalker2_validate -0.584342697115908 1.79074991263117e-11 NA NA NA hsa-miR-200b-3p FEZ2 2.06025765178663 3.07718049337497e-10 -0.457083662710375 2.71711282591411e-05 TargetScan -0.186801519566876 5.00102004604144e-25 NA NA NA hsa-miR-200b-3p FGD1 2.06025765178663 3.07718049337497e-10 -0.0806606542267625 0.632479567235876 TargetScan -0.251341115318483 7.26883256857235e-19 NA NA NA hsa-miR-200b-3p FGF10 2.06025765178663 3.07718049337497e-10 -1.8542097387882 2.08993527096072e-05 TargetScan -0.712312310936586 3.76939499928314e-22 NA NA NA hsa-miR-200b-3p FGF12 2.06025765178663 3.07718049337497e-10 -0.170390023502168 0.492844460583921 TargetScan -0.206614309600595 2.72454763868074e-06 NA NA NA hsa-miR-200b-3p FGF18 2.06025765178663 3.07718049337497e-10 0.577376156575134 0.0408104462212036 TargetScan -0.105225878046072 0.0394084383165484 NA NA NA hsa-miR-192-5p FGF2 2.0867723747501 3.57854249239079e-08 -1.0252152469847 2.26010255795334e-05 miRNAWalker2_validate -0.350526393312018 4.24849619675693e-23 NA NA NA hsa-miR-200b-3p FIGN 2.06025765178663 3.07718049337497e-10 -0.904389390868205 0.00585165623379918 TargetScan -0.45791267927381 3.9123474461321e-16 NA NA NA hsa-miR-21-5p FIGN 2.21515015440792 6.0468197386674e-49 -0.904389390868205 0.00585165623379918 miRNAWalker2_validate -0.573656767586535 1.83474714506736e-07 NA NA NA hsa-miR-21-5p FILIP1L 2.21515015440792 6.0468197386674e-49 -0.175347250548203 0.368391586032627 miRNAWalker2_validate -0.219196634962422 0.000870338603388346 NA NA NA hsa-miR-21-5p FKBP5 2.21515015440792 6.0468197386674e-49 -0.942251915802888 6.84066806283422e-06 miRNAWalker2_validate -0.48215813614059 4.34486747683706e-12 NA NA NA hsa-miR-200b-3p FLI1 2.06025765178663 3.07718049337497e-10 -0.29368802380763 0.109052566113421 TargetScan -0.166774150746005 2.61506754735907e-07 NA NA NA hsa-miR-196a-5p FLNA 5.05945965885472 4.48343841468539e-25 -0.771411398655463 0.0137692934141899 miRNAWalker2_validate -0.248653215955874 8.32875590787624e-13 NA NA NA hsa-miR-21-5p FMOD 2.21515015440792 6.0468197386674e-49 -0.295859195523175 0.211723275513595 miRNAWalker2_validate;miRTarBase -0.245217199162045 0.00224506569316894 19906824;23445447 Gain-of miR-21 function in MSMC and LSMC reduced TGF-beta-induced expression of fibromodulin and TGF-beta-induced factor P < 0.05 and moderately altered the rate of cell growth and caspase-3/7 activity in these cells;Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 °C and 1 aM at 4 °C which corresponds to nine strands of miR-21 in a 15 μL sample and it is capable of distinguishing among miRNA family members ; sarcoma;breast cancer hsa-miR-200b-3p FN1 2.06025765178663 3.07718049337497e-10 1.63571508215028 7.95640379222503e-08 miRNAWalker2_validate;miRTarBase;TargetScan -0.370936995941682 7.92466179566393e-12 24391862 In contrast mesenchymal markers fibronectin and vimentin were significantly downregulated by miR-200 b prostate cancer hsa-miR-192-5p FNBP1 2.0867723747501 3.57854249239079e-08 -0.968853731521844 4.0379880862342e-05 miRNAWalker2_validate -0.366441223667744 3.59795090803688e-27 NA NA NA hsa-miR-21-5p FNBP1 2.21515015440792 6.0468197386674e-49 -0.968853731521844 4.0379880862342e-05 miRNAWalker2_validate -0.491695496704124 4.57520954480045e-10 NA NA NA hsa-miR-200b-3p FOXF1 2.06025765178663 3.07718049337497e-10 -1.29010168370622 4.15319114357515e-07 TargetScan -0.473534046486302 2.20038709881786e-29 NA NA NA hsa-miR-200b-3p FOXF2 2.06025765178663 3.07718049337497e-10 -1.5540000098613 3.6581898003665e-08 TargetScan -0.482405452685898 6.61937343318697e-24 25798833 The miR 200 family and the miR 183~96~182 cluster target Foxf2 to inhibit invasion and metastasis in lung cancers; We therefore identified a novel mechanism whereby the miR-200 family and the miR-183~96~182 cluster inhibit lung cancer invasion and metastasis by targeting Foxf2 metastasis lung cancer hsa-miR-21-5p FOXN3 2.21515015440792 6.0468197386674e-49 -0.810742720031483 3.71770789957724e-09 miRNAWalker2_validate -0.370125103011511 3.90391954016989e-16 NA NA NA hsa-miR-200b-3p FRMD4A 2.06025765178663 3.07718049337497e-10 -0.200561131466576 0.154599868425942 TargetScan -0.242420870885251 5.88414112715224e-26 NA NA NA hsa-miR-200b-3p FRMD6 2.06025765178663 3.07718049337497e-10 -0.439190318770041 0.0686816780837771 TargetScan -0.408112106522538 9.43180435901221e-25 NA NA NA hsa-miR-200b-3p FSTL1 2.06025765178663 3.07718049337497e-10 0.589175839273943 0.00382207095964144 TargetScan -0.317264499810708 2.2313760583841e-20 NA NA NA hsa-miR-192-5p FUT11 2.0867723747501 3.57854249239079e-08 0.319728761354113 0.0137711462950643 miRNAWalker2_validate -0.134944868148386 5.99588690601135e-12 NA NA NA hsa-miR-200b-3p FYN 2.06025765178663 3.07718049337497e-10 -0.13118694976036 0.370511160601143 TargetScan -0.176448438457875 2.99722476456566e-12 NA NA NA hsa-miR-192-5p FZD1 2.0867723747501 3.57854249239079e-08 0.0753981007015279 0.61656364831583 miRNAWalker2_validate -0.137292935739732 2.00935514333215e-09 NA NA NA hsa-miR-192-5p FZD4 2.0867723747501 3.57854249239079e-08 -0.113370323882084 0.480936634316706 miRNAWalker2_validate -0.150355937966884 7.50047534617471e-10 NA NA NA hsa-miR-192-5p FZD7 2.0867723747501 3.57854249239079e-08 -0.147011194184077 0.508590593861751 miRNAWalker2_validate -0.211278644102795 3.97283811601885e-10 NA NA NA hsa-miR-200b-3p GAB1 2.06025765178663 3.07718049337497e-10 -0.826054570409834 2.95181484151435e-08 TargetScan -0.21756089118029 4.92795684674951e-17 NA NA NA hsa-miR-200b-3p GABBR2 2.06025765178663 3.07718049337497e-10 -0.605489398613026 0.176960778882388 TargetScan -0.293367220202624 0.000259930719272217 NA NA NA hsa-miR-200b-3p GALNT2 2.06025765178663 3.07718049337497e-10 0.0309590514667057 0.777920316536723 TargetScan -0.103719900870136 6.93503217766826e-08 NA NA NA hsa-miR-183-5p GAS1 2.08412478942637 1.08931431254487e-10 -1.04737668517358 0.0036431226503507 miRNAWalker2_validate -0.579615734152899 2.8571250749175e-21 NA NA NA hsa-miR-200b-3p GATA2 2.06025765178663 3.07718049337497e-10 -0.653581887509375 0.00159747550269564 TargetScan -0.158875117069992 1.90016916015782e-05 NA NA NA hsa-miR-200b-3p GDF6 2.06025765178663 3.07718049337497e-10 -0.403736179718871 0.376569127647394 TargetScan -0.58646801391443 8.17644331912555e-14 NA NA NA hsa-miR-200b-3p GDNF 2.06025765178663 3.07718049337497e-10 -1.05986535225821 0.000321077395089479 TargetScan -0.28875177105091 3.69647706920399e-08 NA NA NA hsa-miR-200b-3p GIT2 2.06025765178663 3.07718049337497e-10 -0.019873435999501 0.826579600264393 TargetScan -0.133671276224237 1.12907684895426e-18 NA NA NA hsa-miR-200b-3p GJC1 2.06025765178663 3.07718049337497e-10 -0.0478963839809916 0.854609133332298 TargetScan -0.470894342889208 6.68888824817788e-29 NA NA NA hsa-miR-192-5p GLCCI1 2.0867723747501 3.57854249239079e-08 -0.998098561450464 3.66984761021317e-10 miRNAWalker2_validate -0.11734661026095 4.08880821541927e-06 NA NA NA hsa-miR-200b-3p GLCCI1 2.06025765178663 3.07718049337497e-10 -0.998098561450464 3.66984761021317e-10 TargetScan -0.149555287960042 2.78684513474327e-07 NA NA NA hsa-miR-21-5p GLCCI1 2.21515015440792 6.0468197386674e-49 -0.998098561450464 3.66984761021317e-10 miRNAWalker2_validate -0.298469768405141 4.96385683552513e-08 NA NA NA hsa-miR-200b-3p GLI3 2.06025765178663 3.07718049337497e-10 -0.615147701555008 0.0148224102483965 TargetScan -0.483957405916442 3.07747114087143e-33 NA NA NA hsa-miR-200b-3p GLIS2 2.06025765178663 3.07718049337497e-10 0.760680870507226 0.000261285188401114 TargetScan -0.25865500905463 1.18313250230419e-12 NA NA NA hsa-miR-183-5p GLUL 2.08412478942637 1.08931431254487e-10 -1.1030137811041 1.43199220105315e-12 miRNAWalker2_validate -0.1248875366555 2.054847139668e-05 NA NA NA hsa-miR-196a-5p GLUL 5.05945965885472 4.48343841468539e-25 -1.1030137811041 1.43199220105315e-12 miRNAWalker2_validate -0.12315756070285 8.85455349450465e-12 NA NA NA hsa-miR-21-5p GNAQ 2.21515015440792 6.0468197386674e-49 -0.366797789436988 0.0010245125465966 miRNAWalker2_validate -0.156080037034321 3.50915664444775e-05 NA NA NA hsa-miR-192-5p GPC4 2.0867723747501 3.57854249239079e-08 -0.188149462544121 0.37722256005152 miRNAWalker2_validate -0.112746845788055 0.000687905252262054 NA NA NA hsa-miR-200b-3p GPC6 2.06025765178663 3.07718049337497e-10 -0.0536777685148131 0.814647314570525 TargetScan -0.284128771755665 5.9863355336262e-13 NA NA NA hsa-miR-21-5p GPD1L 2.21515015440792 6.0468197386674e-49 -1.07269581488453 9.10955853600853e-14 miRNAWalker2_validate -0.330360811923411 2.49582359901436e-11 NA NA NA hsa-miR-200b-3p GPM6A 2.06025765178663 3.07718049337497e-10 -3.49353111581557 4.10952936768988e-11 TargetScan -0.985236304175053 2.6592698819066e-28 NA NA NA hsa-miR-200b-3p GPR146 2.06025765178663 3.07718049337497e-10 -1.05300131983879 3.19301406494563e-09 TargetScan -0.250477521044042 1.1881177826671e-15 NA NA NA hsa-miR-200b-3p GPR173 2.06025765178663 3.07718049337497e-10 0.236285832262454 0.300156414322761 TargetScan -0.273168494059699 4.56002141384954e-12 NA NA NA hsa-miR-200b-3p GPR27 2.06025765178663 3.07718049337497e-10 -2.46222755352119 1.09764076702222e-07 TargetScan -0.567286902763872 6.06655129301196e-12 NA NA NA hsa-miR-192-5p GPR37 2.0867723747501 3.57854249239079e-08 -0.784678241835113 0.0673137411875174 miRNAWalker2_validate -0.346935605328656 1.52981941393634e-07 NA NA NA hsa-miR-200b-3p GREB1L 2.06025765178663 3.07718049337497e-10 -1.18599359346287 0.00788624523854754 TargetScan -0.337968708941261 2.39826490351051e-05 NA NA NA hsa-miR-200b-3p GREM2 2.06025765178663 3.07718049337497e-10 -2.41694634218495 9.57530301376063e-07 TargetScan -0.650966983729518 5.83407823607555e-14 NA NA NA hsa-miR-192-5p GRIA1 2.0867723747501 3.57854249239079e-08 -1.86501656904484 6.94746142617262e-05 miRNAWalker2_validate -0.459791959098861 1.94392775067077e-10 NA NA NA hsa-miR-200b-3p GRIN2A 2.06025765178663 3.07718049337497e-10 -2.16084479164215 3.33003121849154e-06 TargetScan -0.663279344356061 1.94774443346422e-16 NA NA NA hsa-miR-200b-3p GRIP1 2.06025765178663 3.07718049337497e-10 -0.818995365925745 0.00110036000308407 TargetScan -0.252718589497295 1.34686591638647e-08 NA NA NA hsa-miR-200b-3p GUCY1A3 2.06025765178663 3.07718049337497e-10 0.320384598483079 0.245679823414486 TargetScan -0.452328098297678 4.10073589854362e-23 NA NA NA hsa-miR-192-5p GULP1 2.0867723747501 3.57854249239079e-08 -0.781307951969337 0.00107208390718881 miRNAWalker2_validate -0.118533013079387 0.00164543105330443 NA NA NA hsa-miR-200b-3p HAS2 2.06025765178663 3.07718049337497e-10 0.0573496916277678 0.788936564855642 TargetScan -0.104649390180562 0.00653562765195696 NA NA NA hsa-miR-200b-3p HCFC2 2.06025765178663 3.07718049337497e-10 -0.716945650565211 4.70460742079511e-08 TargetScan -0.224622967909466 3.67656896848055e-24 NA NA NA hsa-miR-200b-3p HDAC4 2.06025765178663 3.07718049337497e-10 -0.328802223196003 0.0475619343153322 TargetScan -0.240059007124108 1.38953567168753e-17 NA NA NA hsa-miR-200b-3p HEG1 2.06025765178663 3.07718049337497e-10 0.241718758295208 0.17358962483006 TargetScan -0.26245029771979 1.88067331361102e-18 NA NA NA hsa-miR-18a-5p HERC1 2.04701000113742 1.30593450319289e-12 -0.195905781077467 0.0854376565297647 miRNAWalker2_validate -0.151540216505898 7.70850363060374e-12 NA NA NA hsa-miR-200b-3p HIPK3 2.06025765178663 3.07718049337497e-10 -0.453460252737316 0.00223114894328011 TargetScan -0.204587896775969 8.68837435752391e-16 NA NA NA hsa-miR-200b-3p HLF 2.06025765178663 3.07718049337497e-10 -2.44260242805055 4.45000537404727e-11 TargetScan -0.7185481952653 2.94512521460051e-31 NA NA NA hsa-miR-192-5p HOOK3 2.0867723747501 3.57854249239079e-08 0.272600395714842 0.0995732135990335 miRNAWalker2_validate -0.23683882489823 2.86840487058371e-23 NA NA NA hsa-miR-183-5p HOXA5 2.08412478942637 1.08931431254487e-10 -0.700496721763648 0.00235992010111307 miRNAWalker2_validate -0.347319919404707 1.77741173192178e-18 NA NA NA hsa-miR-200b-3p HOXA5 2.06025765178663 3.07718049337497e-10 -0.700496721763648 0.00235992010111307 TargetScan -0.310178603283595 5.68005372389173e-15 NA NA NA hsa-miR-200b-3p HS3ST3A1 2.06025765178663 3.07718049337497e-10 0.071423741699323 0.775993889593196 TargetScan -0.281722879818435 1.12078756796853e-10 NA NA NA hsa-miR-192-5p HSPA2 2.0867723747501 3.57854249239079e-08 -1.54334573172528 1.46012961305722e-08 miRNAWalker2_validate -0.437505144941808 1.87496258217843e-28 NA NA NA hsa-miR-1-3p HSPD1 -2.73171785118192 3.4424534921136e-12 1.09026136229175 5.42429006906136e-14 miRTarBase -0.216090768118312 9.44287457144795e-26 NA NA NA hsa-miR-192-5p ID4 2.0867723747501 3.57854249239079e-08 -1.64381994370786 3.62108939915692e-12 miRNAWalker2_validate -0.290354258738131 2.849515978583e-15 NA NA NA hsa-miR-200b-3p IDS 2.06025765178663 3.07718049337497e-10 -0.0995092469681822 0.452237040895841 TargetScan -0.163627855404718 6.60702722108642e-13 NA NA NA hsa-miR-183-5p IGF1R 2.08412478942637 1.08931431254487e-10 0.116961071763603 0.57112438908184 miRNAWalker2_validate -0.108412615681544 0.00383685739243794 NA NA NA hsa-miR-192-5p IGF1R 2.0867723747501 3.57854249239079e-08 0.116961071763603 0.57112438908184 miRNAWalker2_validate -0.147175389757737 3.89731047961255e-06 NA NA NA hsa-miR-194-5p IGF1R 2.24386881692218 9.35116329002209e-11 0.116961071763603 0.57112438908184 miRNAWalker2_validate;miRTarBase -0.139203332288475 5.93692812368786e-05 NA NA NA hsa-miR-200b-3p IGSF3 2.06025765178663 3.07718049337497e-10 -0.696165647878072 0.000303013857178524 TargetScan -0.127076062039306 0.000263731602032531 NA NA NA hsa-miR-192-5p IL1R1 2.0867723747501 3.57854249239079e-08 0.0774863445440346 0.657011719623057 miRNAWalker2_validate -0.143361190621522 8.17071706780348e-08 NA NA NA hsa-miR-192-5p IL6ST 2.0867723747501 3.57854249239079e-08 -0.401511913072166 0.00675820073922165 miRNAWalker2_validate -0.179368286957245 4.34841282521846e-16 NA NA NA hsa-miR-200b-3p IL6ST 2.06025765178663 3.07718049337497e-10 -0.401511913072166 0.00675820073922165 TargetScan -0.225425747172054 1.88662933456835e-19 NA NA NA hsa-miR-200b-3p IMMP2L 2.06025765178663 3.07718049337497e-10 -0.565538828322231 0.000152554189450385 TargetScan -0.109898033224479 4.41644228461287e-05 NA NA NA hsa-miR-200b-3p INPP4A 2.06025765178663 3.07718049337497e-10 0.070243412672542 0.535628784667479 TargetScan -0.14848743391678 1.61520128947153e-14 NA NA NA hsa-miR-192-5p INPP5F 2.0867723747501 3.57854249239079e-08 0.0457326310808011 0.7032738714146 miRNAWalker2_validate -0.140301640107846 3.02355923505294e-15 NA NA NA hsa-miR-200b-3p IQCJ-SCHIP1 2.06025765178663 3.07718049337497e-10 -0.348035812428486 0.134169166438994 TargetScan -0.461830121743074 7.77401758045142e-37 NA NA NA hsa-miR-200b-3p IRS1 2.06025765178663 3.07718049337497e-10 -0.0263168100229603 0.888545775357123 TargetScan -0.178970489896676 5.95671901451481e-08 26314828 Taken together our results suggest that miR-200 may activate AKT in lung adenocarcinoma cells through a FOG2-independent mechanism involving IRS-1 lung cancer hsa-miR-18a-5p IRS4 2.04701000113742 1.30593450319289e-12 -2.36249743886607 2.07501297504123e-05 miRNAWalker2_validate -0.511005690284128 5.83998243326969e-06 NA NA NA hsa-miR-21-5p ISCU 2.21515015440792 6.0468197386674e-49 -0.602780016325892 1.09946571189988e-09 miRNAWalker2_validate;miRTarBase -0.212248702001438 2.24306394488696e-10 NA NA NA hsa-miR-183-5p ITGA5 2.08412478942637 1.08931431254487e-10 0.451610603015354 0.0352433294585663 miRNAWalker2_validate -0.262508236266232 3.15413351773057e-12 NA NA NA hsa-miR-183-5p ITGA8 2.08412478942637 1.08931431254487e-10 -1.91389446067294 3.08011993269904e-08 miRNAWalker2_validate -0.558513541677476 1.6334610963548e-20 NA NA NA hsa-miR-194-5p ITGA9 2.24386881692218 9.35116329002209e-11 -0.825924039856462 0.00174155829133973 miRNAWalker2_validate;miRTarBase -0.304755562790261 2.04883725664225e-12 NA NA NA hsa-miR-183-5p ITGB1 2.08412478942637 1.08931431254487e-10 0.523596429686159 0.000169768671621539 miRNAWalker2_validate;miRTarBase -0.131986789220149 1.4627928835727e-07 NA NA NA hsa-miR-192-5p ITGB1 2.0867723747501 3.57854249239079e-08 0.523596429686159 0.000169768671621539 miRNAWalker2_validate -0.150999493689047 9.20856253461791e-13 NA NA NA hsa-miR-183-5p ITPKB 2.08412478942637 1.08931431254487e-10 -0.703543595422723 0.00105945909085452 miRNAWalker2_validate -0.423524568296436 2.86804480162215e-34 NA NA NA hsa-miR-200b-3p ITPR1 2.06025765178663 3.07718049337497e-10 -0.978205239289609 1.76770291502282e-06 TargetScan -0.354140928419501 5.23323926103515e-25 NA NA NA hsa-miR-200b-3p ITSN1 2.06025765178663 3.07718049337497e-10 -0.174910914321985 0.0772411521730026 TargetScan -0.109462423482328 1.89839791367856e-10 NA NA NA hsa-miR-200b-3p JAKMIP2 2.06025765178663 3.07718049337497e-10 -1.38795790540687 1.75125368830264e-06 TargetScan -0.52555477860223 9.42556314845875e-28 NA NA NA hsa-miR-200b-3p JAKMIP3 2.06025765178663 3.07718049337497e-10 -0.453817101961681 0.0924761400087551 TargetScan -0.272092990552816 9.79194461811902e-09 NA NA NA hsa-miR-200b-3p JAZF1 2.06025765178663 3.07718049337497e-10 -0.376602966601115 0.0267725739241035 TargetScan -0.293911916600013 5.30260186444781e-26 NA NA NA hsa-miR-200b-3p JMY 2.06025765178663 3.07718049337497e-10 -0.265588063934445 0.0620459109443017 TargetScan -0.206801076233742 8.46363040470566e-18 NA NA NA hsa-miR-21-5p JMY 2.21515015440792 6.0468197386674e-49 -0.265588063934445 0.0620459109443017 miRNAWalker2_validate -0.228453213114217 1.56898768866335e-06 NA NA NA hsa-miR-200b-3p JUN 2.06025765178663 3.07718049337497e-10 -0.932062991848233 5.08561574713346e-09 TargetScan -0.127430274428312 1.29168771293238e-05 NA NA NA hsa-miR-200b-3p KANK1 2.06025765178663 3.07718049337497e-10 -0.550274438223532 0.0013376758273072 TargetScan -0.1594750549317 1.71503156751433e-07 NA NA NA hsa-miR-200b-3p KANK2 2.06025765178663 3.07718049337497e-10 -0.932286924516263 1.5601005567271e-05 TargetScan -0.478444346206633 7.70332188506019e-48 NA NA NA hsa-miR-192-5p KAT2B 2.0867723747501 3.57854249239079e-08 -1.46630453221237 1.85020249256189e-18 miRNAWalker2_validate -0.192444824688028 9.08421646367046e-13 NA NA NA hsa-miR-192-5p KATNAL1 2.0867723747501 3.57854249239079e-08 -0.238121876187191 0.227473164378047 miRNAWalker2_validate -0.335836630590676 2.19347995558738e-35 NA NA NA hsa-miR-196a-5p KATNAL1 5.05945965885472 4.48343841468539e-25 -0.238121876187191 0.227473164378047 miRNAWalker2_validate -0.118842782955899 9.05831181579923e-08 NA NA NA hsa-miR-200b-3p KATNAL1 2.06025765178663 3.07718049337497e-10 -0.238121876187191 0.227473164378047 TargetScan -0.356423069845917 4.70082600332586e-29 NA NA NA hsa-miR-21-5p KBTBD7 2.21515015440792 6.0468197386674e-49 -0.0136055168559617 0.919602264955557 miRNAWalker2_validate -0.111793529919025 0.0142527695926195 NA NA NA hsa-miR-200b-3p KCNB1 2.06025765178663 3.07718049337497e-10 -3.20857676127652 3.00924790818105e-08 TargetScan -1.16302126630405 3.28261761258998e-35 NA NA NA hsa-miR-200b-3p KCND2 2.06025765178663 3.07718049337497e-10 1.01569792564355 0.00135256427875141 TargetScan -0.280058284022251 7.52238331765221e-07 NA NA NA hsa-miR-200b-3p KCND3 2.06025765178663 3.07718049337497e-10 -1.25081349685282 8.58949377013892e-05 TargetScan -0.358813297918302 1.85696661392149e-10 NA NA NA hsa-miR-1-3p KCNJ2 -2.73171785118192 3.4424534921136e-12 0.715735490663241 0.0012607689075355 miRTarBase -0.118340155376623 0.000348075112532277 NA NA NA hsa-miR-192-5p KCNMB4 2.0867723747501 3.57854249239079e-08 -0.377768864617567 0.178622044823026 miRNAWalker2_validate -0.284186682402571 2.3229021466011e-11 NA NA NA hsa-miR-133a-3p KCNQ1 -2.63342156915404 5.72661351480585e-12 0.128492375031743 0.689133079037418 miRNAWalker2_validate -0.122358101538187 0.0128387131948567 NA NA NA hsa-miR-200b-3p KCNQ4 2.06025765178663 3.07718049337497e-10 -0.902266691172708 0.00407242110892301 TargetScan -0.439702178787104 3.19957625326618e-16 NA NA NA hsa-miR-192-5p KCNS3 2.0867723747501 3.57854249239079e-08 -0.43705105181568 0.0619110887481087 miRNAWalker2_validate -0.213541007806964 2.39699501538651e-09 NA NA NA hsa-miR-192-5p KCTD12 2.0867723747501 3.57854249239079e-08 0.00787400313561992 0.970648583019239 miRNAWalker2_validate -0.146028740060388 1.016952649164e-05 NA NA NA hsa-miR-192-5p KCTD15 2.0867723747501 3.57854249239079e-08 -0.534965797066081 0.00975039845928537 miRNAWalker2_validate -0.3103328862612 1.27643564499081e-25 NA NA NA hsa-miR-200b-3p KCTD15 2.06025765178663 3.07718049337497e-10 -0.534965797066081 0.00975039845928537 TargetScan -0.328778575667664 2.40335129934816e-21 NA NA NA hsa-miR-192-5p KDELC2 2.0867723747501 3.57854249239079e-08 0.216170389477556 0.0898833025888716 miRNAWalker2_validate -0.144666965324139 2.76739449674176e-14 NA NA NA hsa-miR-200b-3p KIAA0355 2.06025765178663 3.07718049337497e-10 0.222251688753115 0.046704535001662 TargetScan -0.112084749094146 1.05142381086415e-08 NA NA NA hsa-miR-183-5p KIAA0430 2.08412478942637 1.08931431254487e-10 -0.304584793472401 0.00340668402488961 miRNAWalker2_validate -0.175857164640555 4.34989563026152e-24 NA NA NA hsa-miR-200b-3p KIAA0430 2.06025765178663 3.07718049337497e-10 -0.304584793472401 0.00340668402488961 TargetScan -0.131679232779677 2.14834465762948e-13 NA NA NA hsa-miR-200b-3p KIAA1456 2.06025765178663 3.07718049337497e-10 -0.824815382979913 0.00346285725933416 TargetScan -0.165355525377575 0.00117689038831329 NA NA NA hsa-miR-200b-3p KIAA1462 2.06025765178663 3.07718049337497e-10 0.552874008595252 0.00404720415721057 TargetScan -0.23251378670981 3.89438157849664e-12 NA NA NA hsa-miR-192-5p KIDINS220 2.0867723747501 3.57854249239079e-08 -0.00131844135927928 0.988667768580193 miRNAWalker2_validate -0.102153620573114 1.30170732377819e-13 NA NA NA hsa-miR-1-3p KIF2A -2.73171785118192 3.4424534921136e-12 0.342889432442366 0.00194241298945408 miRTarBase -0.106494928927666 2.29811917762755e-11 NA NA NA hsa-miR-183-5p KIF5C 2.08412478942637 1.08931431254487e-10 -0.770271099699533 0.00199021863152459 miRNAWalker2_validate -0.352565363387306 3.63884452903423e-16 NA NA NA hsa-miR-192-5p KIF5C 2.0867723747501 3.57854249239079e-08 -0.770271099699533 0.00199021863152459 miRNAWalker2_validate -0.26595563932561 2.01769685120343e-12 NA NA NA hsa-miR-146b-5p KIT 2.23105707690238 3.83997814011313e-23 -2.18364564416018 1.34619666534478e-12 miRNAWalker2_validate -0.58166429719042 3.24898395259902e-14 NA NA NA hsa-miR-192-5p KLF10 2.0867723747501 3.57854249239079e-08 -0.783161616014165 3.23661985875306e-08 miRNAWalker2_validate -0.191276033935484 2.3017710908313e-19 NA NA NA hsa-miR-200b-3p KLF10 2.06025765178663 3.07718049337497e-10 -0.783161616014165 3.23661985875306e-08 TargetScan -0.260290743180181 1.89797596155367e-28 NA NA NA hsa-miR-200a-3p KLF11 2.02394729844446 2.21240501925011e-08 -0.279313477370207 0.0534824444994751 miRNAWalker2_validate -0.161193239002396 1.47324265939492e-12 NA NA NA hsa-miR-200b-3p KLF11 2.06025765178663 3.07718049337497e-10 -0.279313477370207 0.0534824444994751 miRNAWalker2_validate -0.17223778160254 6.40839798164597e-12 NA NA NA hsa-miR-200b-3p KLF12 2.06025765178663 3.07718049337497e-10 -0.391196935968631 0.0893097621844466 TargetScan -0.272547645001692 9.32948646906076e-12 NA NA NA hsa-miR-135b-5p KLF4 2.80066701698235 2.12546333413447e-11 -1.88610674592481 2.42476920274834e-18 miRNAWalker2_validate -0.121006765598959 0.000337857966070833 NA NA NA hsa-miR-200b-3p KLF9 2.06025765178663 3.07718049337497e-10 -1.42972938246189 7.62390409996413e-11 TargetScan -0.398076308422641 1.07736995687844e-26 NA NA NA hsa-miR-21-5p KLF9 2.21515015440792 6.0468197386674e-49 -1.42972938246189 7.62390409996413e-11 miRNAWalker2_validate -0.641653819250471 8.8137342133806e-19 NA NA NA hsa-miR-200b-3p KLHL14 2.06025765178663 3.07718049337497e-10 -0.843682974908397 0.0541217730637787 TargetScan -0.203593499575582 0.010062796174042 NA NA NA hsa-miR-192-5p KLHL15 2.0867723747501 3.57854249239079e-08 -0.362737945993933 0.012962856301377 miRNAWalker2_validate -0.13099959220988 4.58597189241853e-09 NA NA NA hsa-miR-21-5p KLHL15 2.21515015440792 6.0468197386674e-49 -0.362737945993933 0.012962856301377 miRNAWalker2_validate -0.271943218699736 2.12204817076348e-08 NA NA NA hsa-miR-200b-3p KLHL3 2.06025765178663 3.07718049337497e-10 -0.578181441034985 0.00374713642224286 TargetScan -0.165632854987737 3.31663386635626e-06 NA NA NA hsa-miR-192-5p KPNA5 2.0867723747501 3.57854249239079e-08 -0.275081759540677 0.0683152031438195 miRNAWalker2_validate -0.178003032452795 2.18239408032147e-15 NA NA NA hsa-miR-200b-3p KSR1 2.06025765178663 3.07718049337497e-10 -0.0935264477686886 0.500996355091324 TargetScan -0.113193568873583 4.18392451843884e-06 NA NA NA hsa-miR-200b-3p LAMC1 2.06025765178663 3.07718049337497e-10 0.370285667657678 0.0366283240859651 TargetScan -0.246259940243578 3.71481628992343e-16 NA NA NA hsa-miR-1-3p LARP4 -2.73171785118192 3.4424534921136e-12 0.410432316370399 0.000134132263689673 miRTarBase -0.109980684985604 1.11021174598878e-12 NA NA NA hsa-miR-200b-3p LATS2 2.06025765178663 3.07718049337497e-10 0.159417049044428 0.23040279833664 TargetScan -0.190742258618125 1.98138601494745e-17 NA NA NA hsa-miR-200b-3p LCA5 2.06025765178663 3.07718049337497e-10 0.344794733894058 0.0481852760843641 TargetScan -0.17875475624585 5.519638403367e-09 NA NA NA hsa-miR-192-5p LHFP 2.0867723747501 3.57854249239079e-08 -0.16725244890427 0.413709309444094 miRNAWalker2_validate -0.251851259659309 7.83967038097141e-17 NA NA NA hsa-miR-200b-3p LHFP 2.06025765178663 3.07718049337497e-10 -0.16725244890427 0.413709309444094 TargetScan -0.325780711159206 9.92212710368773e-22 NA NA NA hsa-miR-192-5p LHFPL4 2.0867723747501 3.57854249239079e-08 -1.81431715801582 3.68825383401522e-05 miRNAWalker2_validate -0.292303859222491 2.44737387383583e-05 NA NA NA hsa-miR-21-5p LIFR 2.21515015440792 6.0468197386674e-49 -1.79354293269512 5.04936245466941e-09 miRNAWalker2_validate -0.675845199596711 7.93687305848831e-11 NA NA NA hsa-miR-192-5p LIMCH1 2.0867723747501 3.57854249239079e-08 -0.915408206659925 5.81579486964095e-06 miRNAWalker2_validate -0.18357428097274 4.97741621944863e-09 NA NA NA hsa-miR-21-5p LIMCH1 2.21515015440792 6.0468197386674e-49 -0.915408206659925 5.81579486964095e-06 miRNAWalker2_validate -0.404741372457521 2.52998047781006e-09 NA NA NA hsa-miR-200b-3p LIN7B 2.06025765178663 3.07718049337497e-10 -1.02527483051279 9.39783974005987e-13 TargetScan -0.138151447160607 1.86096234634516e-07 NA NA NA hsa-miR-21-5p LONRF2 2.21515015440792 6.0468197386674e-49 -2.03899615797417 9.63293546267136e-06 miRNAWalker2_validate -1.03389767928222 1.6990485428945e-11 NA NA NA hsa-miR-200b-3p LPAR1 2.06025765178663 3.07718049337497e-10 -1.56606812793793 2.0874874683954e-10 TargetScan -0.352326937227282 6.08851543911358e-16 NA NA NA hsa-miR-200b-3p LPIN1 2.06025765178663 3.07718049337497e-10 -0.293587483978061 0.0393942939219579 TargetScan -0.118037779532526 3.0861648199128e-06 NA NA NA hsa-miR-192-5p LRIG1 2.0867723747501 3.57854249239079e-08 -0.5374742879248 0.00587721628379153 miRNAWalker2_validate -0.126154667074504 3.41125117205796e-05 NA NA NA hsa-miR-200b-3p LRIG1 2.06025765178663 3.07718049337497e-10 -0.5374742879248 0.00587721628379153 TargetScan -0.237917177126363 2.35859157567259e-12 NA NA NA hsa-miR-200b-3p LRP1 2.06025765178663 3.07718049337497e-10 0.0907573600866467 0.623201963667416 TargetScan -0.189915376663552 4.25212126250088e-09 NA NA NA hsa-miR-200b-3p LRP1B 2.06025765178663 3.07718049337497e-10 -2.16345659630435 6.96966627503369e-08 TargetScan -0.5224621159595 1.80558403475425e-13 NA NA NA hsa-miR-192-5p LYRM7 2.0867723747501 3.57854249239079e-08 -0.403426800928447 0.000748648565531885 miRNAWalker2_validate -0.107604610205898 4.81295477986129e-09 NA NA NA hsa-miR-21-5p LYRM7 2.21515015440792 6.0468197386674e-49 -0.403426800928447 0.000748648565531885 miRNAWalker2_validate -0.125290875722158 0.00211275336479156 NA NA NA hsa-miR-192-5p MACF1 2.0867723747501 3.57854249239079e-08 0.0187245853067326 0.903346275968091 miRNAWalker2_validate -0.202678689636768 2.01074555597497e-19 NA NA NA hsa-miR-200b-3p MAF 2.06025765178663 3.07718049337497e-10 -1.25672430426217 1.95182535429058e-12 TargetScan -0.232442602107388 5.17049196553262e-13 NA NA NA hsa-miR-135b-5p MAFB 2.80066701698235 2.12546333413447e-11 -0.0233819798952197 0.898645258592483 miRNAWalker2_validate -0.126983393779143 1.61894731443194e-06 NA NA NA hsa-miR-200b-3p MAP1B 2.06025765178663 3.07718049337497e-10 -0.558714866074475 0.0502101250165206 TargetScan -0.541138644105683 1.70742285853639e-32 NA NA NA hsa-miR-200b-3p MAP2 2.06025765178663 3.07718049337497e-10 -0.497717570068985 0.0674635842749691 TargetScan -0.330601450202759 2.46203336951485e-12 NA NA NA hsa-miR-18a-5p MAP7D1 2.04701000113742 1.30593450319289e-12 -0.417757032591807 0.00158402713713047 miRNAWalker2_validate -0.219915467735183 2.93557819973434e-18 NA NA NA hsa-miR-200b-3p MARCH8 2.06025765178663 3.07718049337497e-10 -0.597317477363774 4.01259740637912e-06 TargetScan -0.139309928775565 1.4692831341423e-09 NA NA NA hsa-miR-200b-3p MBD5 2.06025765178663 3.07718049337497e-10 -0.418283005723191 0.000358917823942013 TargetScan -0.183003824129791 1.87474668108529e-20 NA NA NA hsa-miR-200b-3p MBNL1 2.06025765178663 3.07718049337497e-10 -0.264288076974033 0.031330406144267 TargetScan -0.183115479740923 7.97322084768595e-19 NA NA NA hsa-miR-21-5p MBNL1 2.21515015440792 6.0468197386674e-49 -0.264288076974033 0.031330406144267 miRNAWalker2_validate -0.135203765968342 0.00112555669494875 NA NA NA hsa-miR-192-5p MCAM 2.0867723747501 3.57854249239079e-08 -0.323857758583791 0.133834564119439 miRNAWalker2_validate -0.295629489308951 4.88186861300647e-21 NA NA NA hsa-miR-200b-3p MCC 2.06025765178663 3.07718049337497e-10 -1.00471938976376 1.64318673576847e-06 TargetScan -0.366970725681043 1.07588149883515e-25 NA NA NA hsa-miR-21-5p MEF2A 2.21515015440792 6.0468197386674e-49 -0.299000743634786 0.0124150173611637 miRNAWalker2_validate -0.187899122982137 2.72404798135243e-06 NA NA NA hsa-miR-21-5p MEF2C 2.21515015440792 6.0468197386674e-49 -0.498587747456098 0.014475128958342 miRNAWalker2_validate;miRTarBase -0.227454002726562 0.00101817169687185 NA NA NA hsa-miR-200b-3p MEF2D 2.06025765178663 3.07718049337497e-10 -0.504357600247539 1.81613073647628e-06 TargetScan -0.168029120413218 6.6353408756682e-21 NA NA NA hsa-miR-21-5p MEGF9 2.21515015440792 6.0468197386674e-49 -0.600901388206393 5.01783667124578e-08 miRNAWalker2_validate -0.219388990787695 3.97388718929709e-09 NA NA NA hsa-miR-21-5p MEIS1 2.21515015440792 6.0468197386674e-49 -1.0148748088312 5.58199831086994e-05 miRNAWalker2_validate -0.536557416133542 1.67837729853715e-10 NA NA NA hsa-miR-192-5p MEIS2 2.0867723747501 3.57854249239079e-08 -0.883335732687269 4.11894676730037e-05 miRNAWalker2_validate -0.178893258910726 9.75009546453556e-08 NA NA NA hsa-miR-1-3p MET -2.73171785118192 3.4424534921136e-12 1.57615611374924 2.64556490930012e-12 miRTarBase -0.154629845683453 7.76035855439818e-06 26499783;27169691 Moreover both EGCG and miR-1 mimic inhibited c-MET expression and combination treatment with EGCG and c-MET inhibitor crizotinib had enhanced inhibitory effects on the growth of MG-63 and U-2OS cells;Dual receptor EGFR and c MET inhibition by tumor suppressive miR 1 and miR 206 in head and neck squamous cell carcinoma ; sarcoma;head and neck cancer hsa-miR-192-5p MEX3B 2.0867723747501 3.57854249239079e-08 0.725871921297744 2.11635015696509e-05 miRNAWalker2_validate -0.153542338378413 6.60207072457244e-09 NA NA NA hsa-miR-200b-3p MEX3B 2.06025765178663 3.07718049337497e-10 0.725871921297744 2.11635015696509e-05 TargetScan -0.153574218897037 5.38729628636004e-07 NA NA NA hsa-miR-200b-3p MFAP5 2.06025765178663 3.07718049337497e-10 -1.38773655702249 0.000288777288845266 TargetScan -0.566283218300622 5.08454872408137e-18 NA NA NA hsa-miR-21-5p MKNK2 2.21515015440792 6.0468197386674e-49 -0.817359756676615 2.08516589759381e-11 miRNAWalker2_validate -0.276823985433867 2.54965706847016e-11 NA NA NA hsa-miR-200b-3p MMD 2.06025765178663 3.07718049337497e-10 0.358596402380194 0.0168656186141133 TargetScan -0.211248938604231 1.41711462495963e-16 NA NA NA hsa-miR-146b-5p MMP16 2.23105707690238 3.83997814011313e-23 0.339181683389834 0.222873688272483 miRNAWalker2_validate -0.251314227721671 0.000267723989148661 21823013;26549292 Inhibitory effects of miR 146b 5p on cell migration and invasion of pancreatic cancer by targeting MMP16; Furthermore we found that matrix metalloproteinase 16 MMP16 was a downstream target of miR-146b-5p by dual-luciferase reporter assay; Altogether our findings suggest that miR-146b-5p may be involved in pancreatic cancer cell migration and invasion by targeting MMP16 and miR-146b-5p may be a potential therapeutic target for the pancreatic cancer;Results of western blot assay indicated that miR-146b-5p increased MMP-16 protein expression and showed a decrease of ZNRF3 protein cell migration; pancreatic cancer;sarcoma hsa-miR-200b-3p MMP16 2.06025765178663 3.07718049337497e-10 0.339181683389834 0.222873688272483 TargetScan -0.362457134683772 3.33943366473066e-14 NA NA NA hsa-miR-192-5p MOAP1 2.0867723747501 3.57854249239079e-08 -0.635870544992165 1.26658509837612e-08 miRNAWalker2_validate -0.148788209309688 8.24998808481234e-19 NA NA NA hsa-miR-21-5p MOAP1 2.21515015440792 6.0468197386674e-49 -0.635870544992165 1.26658509837612e-08 miRNAWalker2_validate -0.338773807893189 5.90413589071478e-21 NA NA NA hsa-miR-200b-3p MOCS1 2.06025765178663 3.07718049337497e-10 -1.16957312236881 2.12145289838661e-08 TargetScan -0.326023117257929 1.49678082346333e-19 NA NA NA hsa-miR-21-5p MOXD1 2.21515015440792 6.0468197386674e-49 -0.390155882839977 0.175727823704991 miRNAWalker2_validate -0.264815994480751 0.00680628252121414 NA NA NA hsa-miR-196a-5p MPP2 5.05945965885472 4.48343841468539e-25 -0.979304354602387 0.0006144308249289 miRNAWalker2_validate -0.192444321794715 2.73544152914228e-09 NA NA NA hsa-miR-21-5p MRAP2 2.21515015440792 6.0468197386674e-49 -1.64970042412983 9.30116817421979e-05 miRNAWalker2_validate -0.466435851891185 0.00127896384482684 NA NA NA hsa-miR-192-5p MSN 2.0867723747501 3.57854249239079e-08 -0.0225963425243751 0.884221645798909 miRNAWalker2_validate -0.18470821036508 9.04356450598895e-16 NA NA NA hsa-miR-200b-3p MSN 2.06025765178663 3.07718049337497e-10 -0.0225963425243751 0.884221645798909 TargetScan -0.249946258334778 2.15257262157223e-22 24037528 MiR 200 can repress breast cancer metastasis through ZEB1 independent but moesin dependent pathways; Instead we found that miR-200b repressed several actin-associated genes with the knockdown of the ezrin-radixin-moesin family member moesin alone phenocopying the repression of cell invasion by miR-200b; Moesin was verified to be directly targeted by miR-200b and restoration of moesin in miR-200b-expressing cells was sufficient to alleviate metastatic repression; In breast cancer cell lines and patient samples the expression of moesin significantly inversely correlated with miR-200 expression and high levels of moesin were associated with poor relapse-free survival metastasis;poor survival breast cancer hsa-miR-192-5p MSRB3 2.0867723747501 3.57854249239079e-08 -1.1706465232609 0.000126305414645397 miRNAWalker2_validate -0.539563165395478 1.15236524330651e-37 NA NA NA hsa-miR-192-5p MT1X 2.0867723747501 3.57854249239079e-08 -2.05507506191053 8.36968885566554e-17 miRNAWalker2_validate -0.173858680305887 1.993845233099e-05 NA NA NA hsa-miR-21-5p MTMR12 2.21515015440792 6.0468197386674e-49 -0.253572180104434 0.0236206016681598 miRNAWalker2_validate -0.163265111729 1.39000796232176e-05 NA NA NA hsa-miR-192-5p MTSS1 2.0867723747501 3.57854249239079e-08 -0.810088738785264 0.000353714566592768 miRNAWalker2_validate -0.208890185970704 2.28942522682014e-09 NA NA NA hsa-miR-200b-3p MTSS1L 2.06025765178663 3.07718049337497e-10 -0.0650296407852853 0.747126264282662 TargetScan -0.285361312732457 6.91459830737785e-17 NA NA NA hsa-miR-200b-3p MXD4 2.06025765178663 3.07718049337497e-10 -0.518630488650339 8.01248091177692e-07 TargetScan -0.109877588243222 4.52859499031595e-09 NA NA NA hsa-miR-192-5p MXI1 2.0867723747501 3.57854249239079e-08 -0.986942900915952 1.94101534384788e-11 miRNAWalker2_validate -0.198188222406647 1.00103605957037e-18 NA NA NA hsa-miR-200b-3p MXI1 2.06025765178663 3.07718049337497e-10 -0.986942900915952 1.94101534384788e-11 TargetScan -0.190119656442599 5.76647650620571e-13 NA NA NA hsa-miR-21-5p MYEF2 2.21515015440792 6.0468197386674e-49 -1.50065972644809 0.000144351041165322 miRNAWalker2_validate -0.824433664032106 3.91379859201315e-10 NA NA NA hsa-miR-200b-3p MYLK 2.06025765178663 3.07718049337497e-10 -1.44806395967964 9.65012105905573e-05 TargetScan -0.797611667528977 6.35192067341987e-44 NA NA NA hsa-miR-200b-3p MYO9A 2.06025765178663 3.07718049337497e-10 -0.147200402080686 0.323729766315484 TargetScan -0.211125639834033 6.38546890420585e-17 NA NA NA hsa-miR-21-5p MYO9A 2.21515015440792 6.0468197386674e-49 -0.147200402080686 0.323729766315484 miRNAWalker2_validate -0.157350839286125 0.00178899475396405 NA NA NA hsa-miR-200b-3p MYT1 2.06025765178663 3.07718049337497e-10 -0.563889323774597 0.251181740028895 TargetScan -0.185860772502643 0.0362034243898411 NA NA NA hsa-miR-200b-3p NACC2 2.06025765178663 3.07718049337497e-10 -0.451762729052781 0.0126696300271997 TargetScan -0.328920272002979 4.28994097935262e-29 NA NA NA hsa-miR-200b-3p NANOS1 2.06025765178663 3.07718049337497e-10 -0.224839937186315 0.081535141672364 TargetScan -0.130640511918575 7.76101203825853e-09 NA NA NA hsa-miR-18a-5p NBEA 2.04701000113742 1.30593450319289e-12 -1.40726399632313 0.000274481542791401 miRNAWalker2_validate -0.696223191532435 2.29949416984955e-21 NA NA NA hsa-miR-21-5p NBEA 2.21515015440792 6.0468197386674e-49 -1.40726399632313 0.000274481542791401 miRNAWalker2_validate -0.882548512742303 5.21258264448881e-12 NA NA NA hsa-miR-935 NBEA 2.321600496365 1.00042201726114e-07 -1.40726399632313 0.000274481542791401 miRNAWalker2_validate -0.472261757164365 3.25082672761729e-19 NA NA NA hsa-miR-200b-3p NCAM1 2.06025765178663 3.07718049337497e-10 -2.66813815810839 1.70378058404723e-12 TargetScan -0.683261356797174 4.74869679562918e-26 NA NA NA hsa-miR-200b-3p NCS1 2.06025765178663 3.07718049337497e-10 -0.652620477793938 0.0278454371426777 TargetScan -0.558198966297297 9.2344830055105e-32 NA NA NA hsa-miR-192-5p NDE1 2.0867723747501 3.57854249239079e-08 -1.39508330784898 2.25855054668167e-05 miRNAWalker2_validate -0.539318434849762 8.43976039213558e-31 NA NA NA hsa-miR-200b-3p NDN 2.06025765178663 3.07718049337497e-10 -1.37695704553379 3.45629342707931e-08 TargetScan -0.522912115238464 1.74992887131126e-39 NA NA NA hsa-miR-200b-3p NDNF 2.06025765178663 3.07718049337497e-10 -1.51391923305532 5.24454277418782e-05 TargetScan -0.405458472066243 1.05157371455808e-09 NA NA NA hsa-miR-200b-3p NDST1 2.06025765178663 3.07718049337497e-10 -0.130588786602125 0.314387734671676 TargetScan -0.133836021928855 3.60941822425572e-09 NA NA NA hsa-miR-200b-3p NEGR1 2.06025765178663 3.07718049337497e-10 -2.01758948574649 3.53214332685673e-09 TargetScan -0.661584721763415 8.34578550967993e-32 NA NA NA hsa-miR-192-5p NEK1 2.0867723747501 3.57854249239079e-08 -0.287505938477648 0.00657690319494584 miRNAWalker2_validate -0.145703314458412 2.47324899347785e-21 NA NA NA hsa-miR-21-5p NEK1 2.21515015440792 6.0468197386674e-49 -0.287505938477648 0.00657690319494584 miRNAWalker2_validate -0.131560282088985 0.000229334527223842 NA NA NA hsa-miR-200b-3p NEK10 2.06025765178663 3.07718049337497e-10 -0.78923113131067 7.03147924958596e-05 TargetScan -0.192478608164016 5.591593429512e-08 NA NA NA hsa-miR-200b-3p NEK9 2.06025765178663 3.07718049337497e-10 -0.112532343950376 0.296110755688608 TargetScan -0.155410593546568 1.00557022924511e-17 NA NA NA hsa-miR-200b-3p NFASC 2.06025765178663 3.07718049337497e-10 -1.21285969875073 0.000588897114251105 TargetScan -0.684955870377482 8.99807161679891e-34 NA NA NA hsa-miR-200b-3p NFIA 2.06025765178663 3.07718049337497e-10 -0.546116799329663 0.00406390884761114 TargetScan -0.254040267632355 8.50776863154421e-15 NA NA NA hsa-miR-200b-3p NFIB 2.06025765178663 3.07718049337497e-10 0.0225947120786127 0.907947126450015 TargetScan -0.115124416405723 0.000994512841781149 NA NA NA hsa-miR-200b-3p NFIC 2.06025765178663 3.07718049337497e-10 -0.525555953395095 0.000626295957247341 TargetScan -0.243693173415379 4.13897936548959e-21 NA NA NA hsa-miR-183-5p NHSL2 2.08412478942637 1.08931431254487e-10 -0.397340956842721 0.158978844057479 miRNAWalker2_validate -0.497566645969317 1.34008425527034e-26 NA NA NA hsa-miR-192-5p NIN 2.0867723747501 3.57854249239079e-08 -0.252681320029704 0.137941934206193 miRNAWalker2_validate -0.24046897245338 1.35703690015093e-22 26743688 MiR 192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein prostate cancer hsa-miR-21-5p NIN 2.21515015440792 6.0468197386674e-49 -0.252681320029704 0.137941934206193 miRNAWalker2_validate -0.131946661146631 0.0226486995763727 NA NA NA hsa-miR-200b-3p NKD1 2.06025765178663 3.07718049337497e-10 -0.462280090506528 0.262062739608295 TargetScan -0.302543989129346 3.88399922641002e-05 NA NA NA hsa-miR-192-5p NKX3-1 2.0867723747501 3.57854249239079e-08 -0.537969900637996 0.0339763112751292 miRNAWalker2_validate -0.167683226505431 2.14868694894442e-05 NA NA NA hsa-miR-200b-3p NLGN4X 2.06025765178663 3.07718049337497e-10 -1.0328790909786 0.00177741749681608 TargetScan -0.452110876127727 2.00437539355395e-15 NA NA NA hsa-miR-200b-3p NOG 2.06025765178663 3.07718049337497e-10 -0.00525322557399677 0.988813892893077 TargetScan -0.476852456289133 1.37552568171658e-13 NA NA NA hsa-miR-183-5p NOTCH2 2.08412478942637 1.08931431254487e-10 0.138226358603258 0.351625722215957 miRNAWalker2_validate -0.173408604692044 2.67870355809377e-11 NA NA NA hsa-miR-200b-3p NOVA1 2.06025765178663 3.07718049337497e-10 -2.03955007528378 1.32480844598322e-07 TargetScan -0.695593910747403 4.88643859510498e-27 NA NA NA hsa-miR-200b-3p NOVA2 2.06025765178663 3.07718049337497e-10 0.42427201532203 0.00657550941360171 TargetScan -0.13549802722943 1.05845164426748e-06 NA NA NA hsa-miR-192-5p NPHP3 2.0867723747501 3.57854249239079e-08 -0.51989084361666 0.000175333522053114 miRNAWalker2_validate -0.154073648131723 2.07502939099699e-13 NA NA NA hsa-miR-200b-3p NPTX1 2.06025765178663 3.07718049337497e-10 -2.73557408094059 1.52696712035104e-06 TargetScan -1.06059722801479 1.00674568689959e-29 NA NA NA hsa-miR-192-5p NPTXR 2.0867723747501 3.57854249239079e-08 -1.00186986119569 0.00367721137641764 miRNAWalker2_validate -0.429750733853547 5.78344228820466e-17 NA NA NA hsa-miR-935 NR2F2 2.321600496365 1.00042201726114e-07 -0.138727905922965 0.397897286244025 miRNAWalker2_validate -0.120710377240753 1.85903370611349e-07 NA NA NA hsa-miR-18a-5p NR3C1 2.04701000113742 1.30593450319289e-12 -0.898894495561724 5.37287634787075e-07 miRNAWalker2_validate;miRTarBase -0.311367913026351 2.38499830124315e-19 NA NA NA hsa-miR-200b-3p NR3C1 2.06025765178663 3.07718049337497e-10 -0.898894495561724 5.37287634787075e-07 TargetScan -0.296748234046349 1.59926226265969e-22 NA NA NA hsa-miR-192-5p NREP 2.0867723747501 3.57854249239079e-08 0.530509207259767 0.00332033398775606 miRNAWalker2_validate -0.186658776770056 1.0471573749702e-11 NA NA NA hsa-miR-200b-3p NREP 2.06025765178663 3.07718049337497e-10 0.530509207259767 0.00332033398775606 TargetScan -0.216247817654621 6.63413892669662e-12 NA NA NA hsa-miR-200b-3p NRP2 2.06025765178663 3.07718049337497e-10 -0.239503242524362 0.327696709683055 TargetScan -0.486339113816061 6.17474436248232e-37 NA NA NA hsa-miR-196a-5p NRXN1 5.05945965885472 4.48343841468539e-25 -3.77804960296992 8.78046893071678e-12 miRNAWalker2_validate -0.449557239374727 2.00569876084534e-12 NA NA NA hsa-miR-192-5p NT5M 2.0867723747501 3.57854249239079e-08 -0.674854777150096 0.000470914802451387 miRNAWalker2_validate -0.15088109445808 4.96880207248029e-07 NA NA NA hsa-miR-200b-3p NTF3 2.06025765178663 3.07718049337497e-10 -1.52188285755702 4.13580352197116e-07 TargetScan -0.168627433095034 0.00227099339738329 NA NA NA hsa-miR-21-5p NTF3 2.21515015440792 6.0468197386674e-49 -1.52188285755702 4.13580352197116e-07 miRTarBase -0.741062168553893 1.15482809906199e-13 NA NA NA hsa-miR-192-5p NUCKS1 2.0867723747501 3.57854249239079e-08 0.325102076142334 0.00397222584600734 miRNAWalker2_validate -0.100907081276142 5.22903704582695e-09 NA NA NA hsa-miR-200b-3p NYAP1 2.06025765178663 3.07718049337497e-10 -1.17961202441282 0.000226776631096157 TargetScan -0.328720135513019 7.32370338061405e-09 NA NA NA hsa-miR-192-5p OGFRL1 2.0867723747501 3.57854249239079e-08 -0.44512577168017 0.0218904834741364 miRNAWalker2_validate -0.197906981761335 1.81249494965369e-11 NA NA NA hsa-miR-18a-5p ORAI3 2.04701000113742 1.30593450319289e-12 -0.256859678793534 0.0350545934554395 miRNAWalker2_validate -0.156742473553751 4.68488003226778e-11 NA NA NA hsa-miR-21-5p OSBPL1A 2.21515015440792 6.0468197386674e-49 -1.05361282706133 2.95872705953644e-08 miRNAWalker2_validate -0.411861019921473 1.38017574843007e-10 NA NA NA hsa-miR-192-5p OSBPL6 2.0867723747501 3.57854249239079e-08 -1.15327659051427 2.95657055047018e-06 miRNAWalker2_validate -0.370562627152088 7.40825407149164e-25 NA NA NA hsa-miR-200b-3p OSMR 2.06025765178663 3.07718049337497e-10 1.07319248891458 2.03893889502661e-07 TargetScan -0.1694133215127 6.55580706775718e-06 NA NA NA hsa-miR-200b-3p OSR1 2.06025765178663 3.07718049337497e-10 -0.914235079349368 0.011234198005583 TargetScan -0.381757575805446 1.8160415914766e-09 NA NA NA hsa-miR-21-5p OSR1 2.21515015440792 6.0468197386674e-49 -0.914235079349368 0.011234198005583 miRNAWalker2_validate -0.485551851015141 6.93760637786918e-05 NA NA NA hsa-miR-200b-3p OSTM1 2.06025765178663 3.07718049337497e-10 -0.246334914419587 0.0298039433570488 TargetScan -0.205134337444651 3.29953474911774e-29 NA NA NA hsa-miR-18a-5p PAFAH1B1 2.04701000113742 1.30593450319289e-12 -0.429125161604443 1.79547396100101e-08 miRNAWalker2_validate -0.111849021108107 8.99612519803754e-14 NA NA NA hsa-miR-200b-3p PAIP2 2.06025765178663 3.07718049337497e-10 -0.514527553538336 2.05179039015577e-08 TargetScan -0.1669100829357 4.91219253660578e-28 NA NA NA hsa-miR-200b-3p PAK3 2.06025765178663 3.07718049337497e-10 -0.628036493389936 0.0725313783898835 TargetScan -0.373652609833614 1.11036978399302e-09 NA NA NA hsa-miR-196a-5p PALLD 5.05945965885472 4.48343841468539e-25 -0.733799109511982 0.0016821174230045 miRNAWalker2_validate -0.180362145576339 4.64648676311079e-12 NA NA NA hsa-miR-21-5p PALLD 2.21515015440792 6.0468197386674e-49 -0.733799109511982 0.0016821174230045 miRNAWalker2_validate -0.462238934492261 2.86411397374222e-09 NA NA NA hsa-miR-200b-3p PALM2-AKAP2 2.06025765178663 3.07718049337497e-10 -0.288031326259333 0.0858840552909669 TargetScan -0.282318027114935 1.43597581154505e-24 NA NA NA hsa-miR-200b-3p PAM 2.06025765178663 3.07718049337497e-10 -0.213715233670169 0.151419217579645 TargetScan -0.206106868007026 4.1650250888171e-16 NA NA NA hsa-miR-200b-3p PAPLN 2.06025765178663 3.07718049337497e-10 -0.548479264272031 0.0280496512763077 TargetScan -0.363893775761635 8.92742975312923e-18 NA NA NA hsa-miR-200b-3p PARD3B 2.06025765178663 3.07718049337497e-10 -0.127470625293643 0.42618046220041 TargetScan -0.162298802006921 7.25494256556626e-09 NA NA NA hsa-miR-21-5p PBX1 2.21515015440792 6.0468197386674e-49 -1.20575316450853 1.28079310379343e-07 miRNAWalker2_validate -0.616000869238203 2.36239792296251e-16 NA NA NA hsa-miR-200b-3p PBX3 2.06025765178663 3.07718049337497e-10 -0.281102097769818 0.131536814716469 TargetScan -0.374622355506081 5.26518166211916e-38 NA NA NA hsa-miR-200b-3p PCDH19 2.06025765178663 3.07718049337497e-10 -1.82078557875039 1.37631334790411e-08 TargetScan -0.433530495548917 1.98139817101367e-14 NA NA NA hsa-miR-192-5p PCDH7 2.0867723747501 3.57854249239079e-08 -0.826841168363386 0.0134942869816737 miRNAWalker2_validate -0.38329800947266 2.40150372192992e-14 NA NA NA hsa-miR-200b-3p PCDH7 2.06025765178663 3.07718049337497e-10 -0.826841168363386 0.0134942869816737 TargetScan -0.507314214211285 2.95237817766257e-19 NA NA NA hsa-miR-200a-3p PCDH8 2.02394729844446 2.21240501925011e-08 -0.996776255196674 0.0536605014298841 miRNAWalker2_validate -0.296196334766945 0.000458396100351949 NA NA NA hsa-miR-200b-3p PCDH8 2.06025765178663 3.07718049337497e-10 -0.996776255196674 0.0536605014298841 TargetScan -0.32201277305269 0.000521644821152421 NA NA NA hsa-miR-200b-3p PCMTD1 2.06025765178663 3.07718049337497e-10 -0.481814036975657 7.02844288535814e-05 TargetScan -0.128642069845803 1.96865670417849e-09 NA NA NA hsa-miR-192-5p PCOLCE2 2.0867723747501 3.57854249239079e-08 -2.53758318917856 3.21821768315232e-08 miRNAWalker2_validate -0.520585350450961 1.92890703023688e-13 NA NA NA hsa-miR-200b-3p PCSK2 2.06025765178663 3.07718049337497e-10 -4.70473903822062 1.0090814029853e-15 TargetScan -0.840836317011267 2.56964323402735e-15 NA NA NA hsa-miR-21-5p PDCD4 2.21515015440792 6.0468197386674e-49 -0.887849040960037 3.08157230271797e-09 miRNAWalker2_validate;miRTarBase -0.226453919888007 1.22571285440319e-05 22212233;20372781;23888942;24659669;25575817;25316501;22072622;22267128;22322403;19836969;23604124;25520758;19682430;23272133;25477028;26252635;25994220;25991676;22747440;23827854;23824073;24865582;24902663;24888238;24149370;20978511;17991735;22689922;27355932;19308091;24331411;26169933;22547075;23558936;23201752;20815812;17968323;25310697;21842656;23567619;20581857;23548551;19013014;21685938;26142886;24472409;25973032;26418978;22086602;26212010;25543482;25543261;23904372;27725205;23564788;25400316;26150475;21546206;19509156;27035745;23052036;22353043;19633292;25566594;21412018;19276261;21330826;26864640;22335905;21636706 PDCD4 expression inversely correlated with miR 21 levels in gastric cancers;Clinicopathological and prognostic significance of PDCD4 and microRNA 21 in human gastric cancer; Recent studies have demonstrated that the novel tumor suppressor protein programmed cell death 4 PDCD4 is downregulated in several human solid cancer types and is suppressed by microRNA-21 miR-21; The objectives of this study were: i to establish the clinicopathological and prognostic significance of PDCD4 mRNA and ii to elucidate any correlation between PDCD4 mRNA and miR-21 in gastric cancer; We also performed an association study comparing PDCD4 mRNA and miR-21 in eight cell lines and 49 gastric cancers; An inverse correlation between PDCD4 mRNA and miR-21 was found in gastric cancer; Furthermore our findings suggest that PDCD4 mRNA is negatively regulated by miR-21 in gastric cancer and may serve as a target for effective therapies;Oxidative stress upregulates PDCD4 expression in patients with gastric cancer via miR 21; The expression of miR-21 and PDCD4 was highly correlated with the degree of differentiation tumor staging local lymphatic node metastasis and remote metastasis; In addition the relative expression of PDCD4 was negatively correlated with miR-21; Thus we conclude that ROS promotes gastric carcinogenesis via upregulating miR-21 expression which in turn down-regulates the expression of PDCD4 in gastric cancer cells;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;We identified miR-21 was directly downregulated by RBP2 and found that miR-21 downregulated PDCD4 expression in leukemia cells;MTCs were consistently associated with miR-21 up-regulation P < .0016 and featured significant PDCD4 nuclear down-regulation; An inverse correlation emerged between miR-21 overexpression and PDCD4 down-regulation P = .0013; This study showed in MTCs that miR-21 regulates PDCD4 expression and also that the miR-21/PDCD4 pathway correlates with clinicopathological variables and prognosis;Although microRNA-21 miR-21 is emerging as an oncogene and has been shown to target several tumor suppressor genes including programmed cell death 4 PDCD4 its precise mechanism of action on cancer stem cells CSCs is unclear; Herein we report that FOLFOX-resistant HCT-116 and HT-29 cells that are enriched in CSCs show a 3- to 7-fold upregulation of pre- and mature miR-21 and downregulation of PDCD4; Likewise overexpression of miR-21 in HCT-116 cells achieved through stable transfection led to the downregulation of PDCD4 and transforming growth factor beta receptor 2 TGFβR2;Prognostic significance of PDCD4 expression and association with microRNA 21 in each Dukes' stage of colorectal cancer patients; Furthermore relationships between the PDCD4 mRNA and microRNA-21 miR-21 were evaluated; The PDCD4 mRNA was investigated by the quantitative real-time RT-PCR method and miR-21 was examined by TaqMan microRNA assays; Significant inverse correlations were demonstrated between PDCD4 and miR-21;miR 21 promotes migration and invasion by the miR 21 PDCD4 AP 1 feedback loop in human hepatocellular carcinoma; The expression level of miR-21 was inversely correlated with the protein expression level of a previously identified target gene programmed cell death 4 PDCD4;This short review will focus on our recent finding that the microRNA miR-21 posttranscriptionally regulates Pdcd4 as well as invasion intravasation and metastasis;HBx mediated miR 21 upregulation represses tumor suppressor function of PDCD4 in hepatocellular carcinoma; HBx downregulates PDCD4 expression at least partially through miR-21; Taken together this study reported for the first time that HBx downregulates PDCD4 and upregulates miR-21 expression; The deregulation of PDCD4 by HBx through miR-21 represents a potential novel mechanism of the downregulation of PDCD4 in HBV-related HCC and provides new insights into HCC development;MicroRNA 21 Down regulates Rb1 Expression by Targeting PDCD4 in Retinoblastoma; MicroRNA-21 miR-21 possesses the oncogenic potential to target several tumor suppressor genes including PDCD4 and regulates tumor progression and metastasis; However the mechanism of how miR-21 regulates PDCD4 is poorly understood in RB; Using the TargetScan program we identified a list of potential target genes of these miRNAs of which PDCD4 is one the targets of miR-21; In this study we focused on the regulatory mechanism of miR-21 on PDCD4 in RB; We demonstrated an inverse correlation between miR-21 and PDCD4 expression in Weri-Rb1 and Y79 cells; These data suggest that miR-21 down-regulates Rb1 by targeting PDCD4 tumor suppressor;MicroRNA 21 promotes cell proliferation and down regulates the expression of programmed cell death 4 PDCD4 in HeLa cervical carcinoma cells; In this study we found that the inhibition of miR-21 in HeLa cervical cancer cells caused profound suppression of cell proliferation and up-regulated the expression of the tumor suppressor gene PDCD4;Additionally resveratrol increased the expression of tumor suppressors PDCD4 and maspin which are negatively regulated by miR-21; Short interfering si RNA against PDCD4 attenuated resveratrol's effect on prostate cancer cells and similar effects were observed following over expression of miR-21 with pre-miR-21 oligonucleotides; MiR-21 expression in these cells appeared to be dependent on Akt as LY294002 reduced the levels of miR-21 along with a concurrent increase in PDCD4 expression; These tumor- and metastatic-suppressive effects of resveratrol were associated with reduced miR-21 and pAkt and elevated PDCD4 levels;The expression of miR-21 and PDCD4 mRNA in transfected cells was quantified by real-time polymerase chain reaction and the expression of PDCD4 protein by Western blot; Cell proliferation apoptosis migration and invasion assays were performed to determine the biological effects of miR-21 expression and PDCD4 inhibition; Co-transfection of miR-21-sponge with PDCD4 siRNA upregulated miR-21 expression in these cells; PDCD4 mRNA and protein levels were increased 2.14-fold and 2.16-fold respectively following inhibition of miR-21 expression; Depletion of PDCD4 by siRNA transfection reversed the reduction of cell proliferation migration and invasion induced by inhibition of miR-21 in A549 cells; It indicates that miR-21 is highly expressed in patients with NSCLC and inhibition of miR-21 expression reduces proliferation migration and invasion of A549 cells by upregulating PDCD4 expression; Modulation of miR-21 or PDCD4 expression may provide a potentially novel therapeutic approach for NSCLC;IL 6 Inhibits the Targeted Modulation of PDCD4 by miR 21 in Prostate Cancer; In this study we evaluated the role of IL-6 in PDCD4 gene expression and how the microRNA miR-21 regulates this process in prostate cancer cell lines PC-3 and LNCaP; The expression of PDCD4 was inhibited by IL-6 but this effect was weakened in cell lines with low expression of miR-21; These findings support the feasibility of future efforts for diagnosis and gene therapy for prostate cancer that are based on IL-6 miR-21 and PDCD4;Furthermore the absence of miR-21 increased the expression of its target gene PDCD4 and subsequently modulated nuclear factor NF-κB activation;In this study using reverse phase protein arrays RPPAs we assessed MPS1 mediated cell signaling pathways and demonstrated that inhibiting MPS1 could upregulate the expression of the tumor suppressor PDCD4 and MSH2 genes by down regulating micro RNA-21 miR-21; We also show the prognostic effect of miR21 PDCD4 and MSH2 levels to patient survival across different GBM molecular subtypes;We also analyzed the immunohistochemical expression of PDCD4 an miR-21 gene target; PDCD4 expression was significantly downregulated in MTC samples consistent with miR-21 upregulation;Aldose reductase inhibition suppresses colon cancer cell viability by modulating microRNA 21 mediated programmed cell death 4 PDCD4 expression; Further AR inhibition also increased PDCD4 a putative target of miR-21 in human colon cancer cells; Collectively these results indicate that AR inhibition prevents growth factor-induced colon cancer growth by down-regulating miR-21 expression and increasing PDCD4 levels through the reactive oxygen species ROS/AMPK/mTOR/AP1/4E-BP1 pathway;Berberine could inhibit miR-21 expression and function in ovarian cancer as shown by an enhancement of its target PDCD4 an important tumor suppressor in ovarian cancer;Overexpression of miR-21 in cisplatin sensitive cells decreased PDCD4 levels and increased cell proliferation;MicroRNA 21 miR 21 post transcriptionally downregulates tumor suppressor PDCD4 and promotes cell transformation proliferation and metastasis in renal cell carcinoma; MiR-21 induces neoplastic transformation cell proliferation and metastasis and downregulates programmed cell death4 PDCD4 in some cancers; The aim of this study was to investigate the roles and interactions of PDCD4 and miR-21 in human renal cell carcinoma RCC; The expression levels of PDCD4 protein and mRNA and miR-21 were examined by Western blot analysis and by qRT-PCR and luciferase reporter assays; MiR-21 not only promoted cancer cell hyperplasia and contributed to tumor cell transformation and metastasis but also post-transcriptionally downregulated PDCD4 protein expression; PDCD4 and miR-21 expression levels potentially play an important role in renal cell cancer;Programmed cell death 4 PDCD4 is a tumor suppressor gene whose expression is directly controlled by microRNA-21 miR-21; This PDCD4 and miR-21 inverse expression was also noted in cells and exosomes from OSC peritoneal effusions compared with nonneoplastic effusions; PDCD4 and miR-21 are involved in OSC oncogenesis;Finally in a subgroup of tumor specimens ROC curve analysis performed on quantitative PCR data sets for miR-21 ITGβ4 and PDCD4 shows that the combination of high miR-21 with low ITGβ4 and PDCD4 expression is able to predict presence of metastasis; In conclusion miR-21 is a key player in oncogenic EMT its overexpression is controlled by the cooperation of genetic and epigenetic alterations and its levels along with ITGβ4 and PDCD4 expression could be exploited as a prognostic tool for CRC metastasis;Transfection of anti-miR-21 rendered HCC cells sensitive to IFN-α/5-FU and such sensitivity was weakened by transfection of siRNAs of target molecules PETN and PDCD4 miR-21 expression in clinical HCC specimens was significantly associated with the clinical response to the IFN-α/5-FU combination therapy and survival rate;Programmed cell death 4 PDCD4 is an important functional target of the microRNA miR 21 in breast cancer cells;In ZD esophagus and tongue oncogenic miR-31 and miR-21 overexpression was accompanied by down-regulation of their respective tumor-suppressor targets PPP2R2A and PDCD4;Due to inhibition of miR-21 expression of the programed cell death 4 PDCD4 gene was promoted in tumors resulting in the induction of apoptosis of tumor cells;We also demonstrated that BMP-6-induced downregulation of miR-21 modified the activity of PDCD4 3'UTR and inhibited MDA-MB-231 cell invasion;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;The results indicated that the miR-21 inhibition could induce the cell cycle arrest in G1 phase upregulate the expression of Programmed Cell Death Protein 4 PDCD4 and thus active the caspase-3 apoptosis pathway;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;Furthermore miR-21 mimic or inhibitor significantly reduced or increased the expression of PDCD4 and TPM1; MiR-21 is overexpressed in RCC tissue and modulates the growth apoptosis and cell cycle progression of RCC cells and regulates the expression of PDCD4 and TPM1;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;Curcumin treatment reduced miR-21 promoter activity and expression in a dose-dependent manner by inhibiting AP-1 binding to the promoter and induced the expression of the tumour suppressor Pdcd4 programmed cell death protein 4 which is a target of miR-21; Taken together this is the first paper to show that curcumin inhibits the transcriptional regulation of miR-21 via AP-1 suppresses cell proliferation tumour growth invasion and in vivo metastasis and stabilizes the expression of the tumour suppressor Pdcd4 in colorectal cancer;MicroRNA 21 miR 21 post transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion intravasation and metastasis in colorectal cancer; This is the first study to show that Pdcd4 is negatively regulated by miR-21;Inflammation and MiR 21 pathways functionally interact to downregulate PDCD4 in colorectal cancer; These findings indicate that miR-21 is a component of the COX-2 inflammation pathway and that this pathway promotes worsening of disease stage in colorectal cancer by inducing accumulation of PGE2 and increasing expression of miR-21 with consequent downregulation of the tumour suppressor gene PDCD4;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Interestingly NVP-LDE-225 induced PDCD4 and apoptosis and inhibited cell viability by suppressing miR-21;PDCD4 a direct target gene of miR-21 could mediate chemoresistance to docetaxel in PC3 cells;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;MicroRNA 21 down regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G; Expression of PDCD4 protein correlates inversely with expression of miR-21 in a number of human glioblastoma cell lines such as T98G A172 U87 and U251; Inhibition of miR-21 increases endogenous levels of PDCD4 in cell line T98G and over-expression miR-21 inhibits PDCD4-dependent apoptosis;Treatment of HSC-3 cells with Nanog- and/or Stat-3-specific small interfering RNAs effectively blocks HA-mediated Nanog-Stat-3 signaling events abrogates miR-21 production and increases PDCD4 expression;Programmed cell death 4 PDCD4 is critical in mediating apoptosis in GBM and is downregulated by miR-21 which may mediate the resistance of glioblastoma cells against chemotherapy or radiation via its target genes PDCD4; This review will focus on the roles of miRNAs family members particularly miR-21 and its target gene PDCD4 in tumors like glioblastoma and new targeting strategies as examples some new targeting therapeutic methods and molecular mechanisms of signal pathways in glioblastoma therapeutics to give the reader the current trends of approach to target regulation of these miRNA and genes for future glioma therapies;Using Western blot analysis miR-21 knockdown enhanced the expression of tumor suppressor PDCD4 and attenuated apoptosis inhibitor c-IAP2;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;METHODS 3-45-dimethylthiazol-2-yl-25-diphenyltetrazolium bromide MTT assay was used to determine the effect of 5-FU on the viability of RKO cells with knockout of miR-21 or high expression of PDCD4;The effects were accompanied by up-regulation of tumor suppressor PDCD4 a known miR-21 target; as well as up-regulation of cofilin and profilin two key proteins involved in actin polymerization and cytoskeleton maintenance as a consequence of miR-182 down-regulation;Increased levels of miR-21 in turn downregulate the expression of the metastasis-suppressor protein programmed cell death 4 PDCD4 a validated miR-21 target;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;In this study we analyzed the expression of miR-21 and of its target PDCD4 Programmed Cell Death 4 during normal hematopoietic differentiation and in AMLs;MiR 21 mediates the radiation resistance of glioblastoma cells by regulating PDCD4 and hMSH2; In addition miR-21 knockdown increased the expression of endogenous PDCD4 and hMSH2 which contributed to the apoptosis and G2 arrest of T98G cells; The findings suggested that miR-21 may mediate the resistance of glioblastoma cells against radiation via its target genes PDCD4 and hMSH2;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated;The expression of programmed cell death-4 PDCD4 was increased by I3C and reduced by miR-21 transfection; Such enhanced chemosensitivity might be explained by the increased expression of PDCD4 which is a downstream target which miR-21 negatively regulates;It has been reported that miR-21 was upregulated in most malignant cancers the proposed mechanism of which was through suppressing expression of programmed cell death 4 PDCD4; In present study it is firstly reported that miR-21 was upregulated in Kazakh's ESCC and that miR-21 played a negative role in regulating PDCD4 using in situ hybridization ISH and luciferase reporter approach;To measure levels of microRNA miR-21 and its target gene programmed cell death 4 PDCD4 in samples of human cutaneous malignant melanoma and normal non-malignant control skin; Relative levels of miR-21 and PDCD4 mRNA were measured using a quantitative real-time reverse transcription-polymerase chain reaction; Compared with normal skin samples the relative level of miR-21 was significantly higher and the relative level of PDCD4 mRNA was significantly lower in the melanoma specimens; A significant negative correlation between PDCD4 mRNA and miR-21 was demonstrated in malignant melanoma r = -0.602; Elevated miR-21 and reduced PDCD4 mRNA levels were both significantly correlated with increased tumour size a higher Clark classification level and the presence of lymph node metastases in malignant melanoma; These findings suggest that miR-21 and PDCD4 might be potential biomarkers for malignant melanoma and might provide treatment targets in the future;MicroRNA 21 and PDCD4 expression in colorectal cancer; Studies have shown that miR-21 exerts its oncogenic activity by targeting the PDCD4 tumour suppressor 3'-UTR; The purpose of this study was to delineate the interaction between PDCD4 and its oncogenic modulator miR-21 in colorectal cancer; A cohort of 48 colorectal tumours 61 normal tissues and 7 polyps were profiled for miR-21 and PDCD4 gene expression; A significant inverse relationship between miR-21 and PDCD4 gene expression p < 0.001 was identified by RT-qPCR; In addition significant reduction of PDCD4 p < 0.001 expression and reciprocal upregulation of miR-21 p = 0.005 in a progressive manner from tumour-polyp-normal mucosae was identified; This study demonstrates the inverse relationship between miR-21 and PDCD4 thus suggesting that miR-21 post-transcriptionally modulates PDCD4 via mRNA degradation;Expression patterns of miR-21 RNA and its target tumor suppressor protein PDCD4 were mutually exclusive;Exosome shuttling microRNA 21 promotes cell migration and invasion targeting PDCD4 in esophageal cancer;Fenhexamid and fludioxonil stimulated miR-21 expression in a concentration-dependent manner and reduced the expression of miR-21 target Pdcd4 protein; Antisense to miR-21 blocked the increase in Pdcd4 protein by fenhexamid and fludioxonil;The expression of programmed cell death 4 PDCD4 which is a miR-21 targeting apoptotic gene has also been enhanced by UA; And over-expression of miR-21 in U251 cells abolished the enhancement of PDCD4 protein by UA;This process leads to microRNA-21 miR-21 production and a tumor suppressor protein e.g PDCD4 program cell death 4 reduction; Transfection of MCF-7 cells with PKCepsilon or Nanog-specific small interfering RNAs effectively blocks HA-mediated PKCepsilon-Nanog signaling events abrogates miR-21 production and increases PDCD4 expression/eIF4A binding;Through dual-luciferase method it was verified that PDCD4 and PDCD10 were real target genes of miR-21 and miR-200c respectively;miR-21 and miR-155 expression was assessed in tumor tissue and in adjacent normal tissue of 156 CRC patients by TaqMan MicroRNA assays and PDCD4 and TP53INP1 mRNA levels were measured by quantitative real-time reverse transcriptase PCR RT-PCR; Significant inverse correlations were demonstrated between miR-21 and PDCD4 mRNA and miR-155 and TP53INP1 mRNA;The aim of this study was a to determine a role of microRNA-21 in esophageal squamous cell carcinoma and b to elucidate the regulation of the programmed cell death 4 PDCD4 gene by microRNA-21; In addition the regulation of PDCD4 by microRNA-21 was elucidated to identify the mechanisms of this regulation; The PDCD4 protein levels in esophageal squamous cell carcinoma cells have an inverse correlation with microRNA-21 expression; MicroRNA-21 targets PDCD4 at the posttranscriptional level and regulates cell proliferation and invasion in esophageal squamous cell carcinoma;Maspin Pdcd4 and miR-21 expressions were evaluated by a real-time polymerase chain reaction in 20 endometrial cancer and 10 normal endometrium samples; Comparison between IA and more advanced International Federation of Gynecology and Obstetrics stages of endometrial cancer in regard to expression levels of maspin Pdcd4 and miR-21 did not reveal any significant differences;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;Sequence prediction and gene expression regulation assays showed that miR-21 could reversely regulate the expression of PDCD4 P < 0.01; Suppression of miR-21 expression is associated with an elevation of Pdcd4 resulting in a significant reduction of proliferation and the apoptosis rate 2.6% was increased to 10.9%; Moreover the anti-proliferation and pro-apoptotic effect by miR-21 suppression could be reversed by PDCD4 knock down;Downregulation of Pdcd4 by mir 21 facilitates glioblastoma proliferation in vivo; We demonstrate that downregulation of mir-21 in glioblastoma-derived cell lines results in increased expression of its target programmed cell death 4 Pdcd4 a known tumor-suppressor gene; In addition our data indicate that either downregulation of mir-21 or overexpression of its target Pdcd4 in glioblastoma-derived cell lines leads to decreased proliferation increased apoptosis and decreased colony formation in soft agar; Using a glioblastoma xenograft model in immune-deficient nude mice we observe that glioblastoma-derived cell lines in which mir-21 levels are downregulated or Pdcd4 is over-expressed exhibit decreased tumor formation and growth; These critical in vivo findings demonstrate an important functional linkage between mir-21 and Pdcd4 and further elucidate the molecular mechanisms by which the known high level of mir-21 expression in glioblastoma can attribute to tumorigenesis--namely inhibition of Pdcd4 and its tumor-suppressive functions ;;staging;metastasis;differentiation;tumorigenesis;;;worse prognosis;;staging;;metastasis;;metastasis;progression;;;;;;poor survival;;;;;metastasis;;metastasis;poor survival;drug resistance;;;;;drug resistance;;;progression;;metastasis;metastasis;staging;;;drug resistance;;;;drug resistance;;;;;metastasis;;differentiation;drug resistance;;;malignant trasformation;malignant trasformation;metastasis;progression;;cell migration;;;;;;;staging;drug resistance;;tumorigenesis gastric cancer;gastric cancer;gastric cancer;gastric cancer;chronic myeloid leukemia;thyroid cancer;colon cancer;colorectal cancer;liver cancer;colon cancer;liver cancer;retinoblastoma;cervical and endocervical cancer;prostate cancer;lung squamous cell cancer;prostate cancer;colorectal cancer;glioblastoma;thyroid cancer;colon cancer;ovarian cancer;ovarian cancer;kidney renal cell cancer;ovarian cancer;colorectal cancer;liver cancer;breast cancer;esophageal cancer;glioblastoma;breast cancer;lung squamous cell cancer;breast cancer;breast cancer;kidney renal cell cancer;glioblastoma;colorectal cancer;colorectal cancer;colorectal cancer;lung cancer;prostate cancer;prostate cancer;B cell lymphoma;glioblastoma;head and neck cancer;glioblastoma;ovarian cancer;liver cancer;colon cancer;breast cancer;breast cancer;B cell lymphoma;acute myeloid leukemia;glioblastoma;prostate cancer;pancreatic cancer;esophageal cancer;melanoma;colorectal cancer;colorectal cancer;esophageal cancer;breast cancer;glioblastoma;breast cancer;breast cancer;colorectal cancer;esophageal cancer;endometrial cancer;pancreatic cancer;lung squamous cell cancer;glioblastoma hsa-miR-192-5p PDE2A 2.0867723747501 3.57854249239079e-08 -1.60248133935421 4.94483694901693e-10 miRNAWalker2_validate -0.342811496465308 2.2104300980193e-18 NA NA NA hsa-miR-18a-5p PDE4D 2.04701000113742 1.30593450319289e-12 -0.501529812759653 0.00928430510499284 miRNAWalker2_validate -0.279245973365489 8.82836095559533e-14 NA NA NA hsa-miR-200b-3p PDE7B 2.06025765178663 3.07718049337497e-10 -1.29539183024445 1.64484072041103e-05 TargetScan -0.516831622112932 8.04451430109395e-25 NA NA NA hsa-miR-21-5p PDGFD 2.21515015440792 6.0468197386674e-49 -1.14874460605402 8.02733598895641e-07 miRNAWalker2_validate -0.377074482110946 2.14599069029555e-06 NA NA NA hsa-miR-192-5p PDLIM3 2.0867723747501 3.57854249239079e-08 -1.37109689119218 2.54104405288571e-05 miRNAWalker2_validate -0.527976606659045 4.68334924766833e-30 NA NA NA hsa-miR-183-5p PEAK1 2.08412478942637 1.08931431254487e-10 0.498401249194297 3.01095206451051e-05 miRNAWalker2_validate -0.114866410431794 9.93417353006958e-08 NA NA NA hsa-miR-21-5p PER3 2.21515015440792 6.0468197386674e-49 -1.21493267564586 1.08869327262652e-08 miRNAWalker2_validate -0.49883605660383 2.85436632603892e-12 NA NA NA hsa-miR-200b-3p PFN2 2.06025765178663 3.07718049337497e-10 -0.308124594300883 0.268763972752918 TargetScan -0.297554388705208 1.03012453965708e-09 NA NA NA hsa-miR-21-5p PGRMC2 2.21515015440792 6.0468197386674e-49 -0.829994806645235 1.26502386117404e-18 miRNAWalker2_validate -0.22005475567961 2.34469526046833e-11 NA NA NA hsa-miR-200b-3p PHACTR3 2.06025765178663 3.07718049337497e-10 -0.760898446997406 0.0651038398581359 TargetScan -0.194945329476753 0.00883060443619837 NA NA NA hsa-miR-192-5p PHF10 2.0867723747501 3.57854249239079e-08 -0.118034981153644 0.293969343023263 miRNAWalker2_validate -0.121691340769862 4.56039736187872e-13 NA NA NA hsa-miR-200b-3p PHF21A 2.06025765178663 3.07718049337497e-10 -0.314340471617779 0.00621059979506441 TargetScan -0.206900602949406 1.06629659174374e-28 NA NA NA hsa-miR-200b-3p PHF21B 2.06025765178663 3.07718049337497e-10 -1.94296466828798 2.59177331886843e-05 TargetScan -0.706650141469302 3.52466567318501e-19 NA NA NA hsa-miR-200b-3p PHLDB1 2.06025765178663 3.07718049337497e-10 0.238938319482238 0.132122406244892 TargetScan -0.242654371116382 7.10759493249702e-20 NA NA NA hsa-miR-192-5p PHTF2 2.0867723747501 3.57854249239079e-08 0.581604321532215 2.7972387423281e-07 miRNAWalker2_validate -0.109363781022406 4.68953128914201e-10 NA NA NA hsa-miR-21-5p PIK3R1 2.21515015440792 6.0468197386674e-49 -0.132584613334835 0.368540743016032 miRNAWalker2_validate -0.149170797978773 0.00275496702870304 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-133a-3p PIK3R2 -2.63342156915404 5.72661351480585e-12 0.6802233434179 1.14371526174319e-06 miRNAWalker2_validate -0.113207314566063 1.18348525180172e-07 NA NA NA hsa-miR-192-5p PIM1 2.0867723747501 3.57854249239079e-08 -1.11369789456653 7.42912371797285e-11 miRNAWalker2_validate -0.125812320360565 4.57886158777155e-06 NA NA NA hsa-miR-200b-3p PIP4K2A 2.06025765178663 3.07718049337497e-10 0.0497046247727253 0.656960215436939 TargetScan -0.145799976846135 2.22527893941728e-14 NA NA NA hsa-miR-192-5p PIP4K2B 2.0867723747501 3.57854249239079e-08 0.176396912458267 0.06975976166235 miRNAWalker2_validate -0.103001064146051 1.58975324185675e-12 NA NA NA hsa-miR-200b-3p PIP4K2B 2.06025765178663 3.07718049337497e-10 0.176396912458267 0.06975976166235 TargetScan -0.13402459650587 4.4534617959894e-16 NA NA NA hsa-miR-192-5p PITPNB 2.0867723747501 3.57854249239079e-08 0.0720431781897597 0.527037368114435 miRNAWalker2_validate -0.105520231928809 9.62989185997897e-10 NA NA NA hsa-miR-200b-3p PITPNM3 2.06025765178663 3.07718049337497e-10 -1.63914377631659 3.3142339868577e-07 TargetScan -0.39642604073762 3.28390154622178e-12 NA NA NA hsa-miR-200b-3p PKD1 2.06025765178663 3.07718049337497e-10 -0.179389262603771 0.299404915965228 TargetScan -0.294552888291482 2.33820900238004e-25 NA NA NA hsa-miR-21-5p PKD2 2.21515015440792 6.0468197386674e-49 -0.239902831398395 0.217409599623742 miRNAWalker2_validate -0.284314803867733 1.31368399072314e-05 NA NA NA hsa-miR-200b-3p PKIA 2.06025765178663 3.07718049337497e-10 -1.26431427605 5.26373640576521e-06 TargetScan -0.456484235724047 2.4040542639679e-22 NA NA NA hsa-miR-21-5p PLAT 2.21515015440792 6.0468197386674e-49 -0.559228051840959 0.00104135060013876 miRNAWalker2_validate;miRTarBase -0.187692286863916 0.00124635653842505 19308091 We also demonstrated that both deltaEF1 and TPA induced miR-21 expression breast cancer hsa-miR-200b-3p PLCL1 2.06025765178663 3.07718049337497e-10 -0.868784420124132 0.000104162683697075 TargetScan -0.421479899586077 4.06733605756339e-31 NA NA NA hsa-miR-200b-3p PLCXD3 2.06025765178663 3.07718049337497e-10 -3.47820333039959 1.97747441352297e-11 TargetScan -0.671794173420585 5.94998609586976e-13 NA NA NA hsa-miR-192-5p PLEKHG2 2.0867723747501 3.57854249239079e-08 0.649639224953562 1.47814580043559e-05 miRNAWalker2_validate -0.105850536903389 6.94688839027781e-06 NA NA NA hsa-miR-200b-3p PLEKHM3 2.06025765178663 3.07718049337497e-10 -0.264766226099178 0.0391508799138563 TargetScan -0.184162958317684 2.46449741384261e-17 NA NA NA hsa-miR-183-5p PLOD2 2.08412478942637 1.08931431254487e-10 0.0206859895158393 0.906042216365495 miRNAWalker2_validate -0.148227505860964 2.31234111399076e-06 NA NA NA hsa-miR-192-5p PLS3 2.0867723747501 3.57854249239079e-08 0.302710860036512 0.0214867475151982 miRNAWalker2_validate -0.115329241982359 1.04819291008112e-08 NA NA NA hsa-miR-200b-3p PLXNA4 2.06025765178663 3.07718049337497e-10 0.21299044346966 0.551620295758605 TargetScan -0.474537186664197 9.46990842562096e-15 NA NA NA hsa-miR-1-3p PNP -2.73171785118192 3.4424534921136e-12 -0.185915552567808 0.260223330520435 miRTarBase -0.104453261173907 1.64536547088838e-05 22378464 PTMA and PNP were identified as new target genes regulated by the miR-1 and miR-133a cluster in BC bladder cancer hsa-miR-200b-3p POLK 2.06025765178663 3.07718049337497e-10 -0.188751167312746 0.0924696692852844 TargetScan -0.102560157916386 2.07419670429958e-07 NA NA NA hsa-miR-200b-3p POU6F1 2.06025765178663 3.07718049337497e-10 -0.860006522186688 1.36739727981555e-05 TargetScan -0.416532307174815 2.42244104276495e-41 NA NA NA hsa-miR-21-5p PPARA 2.21515015440792 6.0468197386674e-49 -0.378523693756559 0.00547606509752035 miRNAWalker2_validate;miRTarBase -0.152463272992749 0.000977751305593663 22244963 In the final integrative analysis of lung cancer related miR-21-targets analysis 24 hub genes were identified by overlap calculation suggesting that miR-21 may play an important role in the development and progression of lung cancer through JAK/STAT signal pathway MAPK signaling pathway Wnt signaling pathway cell cycle PPAR signaling pathway apoptosis pathway and other pathways progression lung cancer hsa-miR-200b-3p PPFIBP1 2.06025765178663 3.07718049337497e-10 0.0806674750313388 0.54797471582341 TargetScan -0.10400011306473 1.24350553825532e-05 NA NA NA hsa-miR-192-5p PPM1A 2.0867723747501 3.57854249239079e-08 -0.623302585337666 5.30525296218818e-12 miRNAWalker2_validate -0.10289857860824 2.4759358529833e-13 NA NA NA hsa-miR-200b-3p PPM1F 2.06025765178663 3.07718049337497e-10 -0.0631875406709161 0.564184144281478 TargetScan -0.171024566761045 5.24017186546818e-21 NA NA NA hsa-miR-21-5p PPM1L 2.21515015440792 6.0468197386674e-49 -0.537351176754866 0.00615520293012723 miRNAWalker2_validate -0.338741437320256 2.52918687319341e-07 NA NA NA hsa-miR-18a-5p PPP1CB 2.04701000113742 1.30593450319289e-12 -0.697343226432961 3.54901349210579e-12 miRNAWalker2_validate -0.145681749590503 5.79254854572247e-13 NA NA NA hsa-miR-192-5p PPP1CB 2.0867723747501 3.57854249239079e-08 -0.697343226432961 3.54901349210579e-12 miRNAWalker2_validate -0.16403578286484 4.53367132936433e-29 NA NA NA hsa-miR-200b-3p PPP1CB 2.06025765178663 3.07718049337497e-10 -0.697343226432961 3.54901349210579e-12 TargetScan -0.150717204905338 1.28057849740968e-17 NA NA NA hsa-miR-192-5p PPP1R12A 2.0867723747501 3.57854249239079e-08 -0.344541774510097 0.0204267785092073 miRNAWalker2_validate -0.229511733680736 1.19582084325733e-27 NA NA NA hsa-miR-200b-3p PPP1R12B 2.06025765178663 3.07718049337497e-10 -1.13914697277207 0.00076065186660822 TargetScan -0.61032224475182 3.15745635337338e-28 NA NA NA hsa-miR-200b-3p PPP1R18 2.06025765178663 3.07718049337497e-10 0.0679340646748354 0.656374261269267 TargetScan -0.20881215477652 8.80771050099062e-16 NA NA NA hsa-miR-200b-3p PPP1R9A 2.06025765178663 3.07718049337497e-10 -0.902560277535807 0.0425430977023791 TargetScan -0.270760624645925 0.00071561366648627 NA NA NA hsa-miR-200b-3p PPP2R2C 2.06025765178663 3.07718049337497e-10 -0.958837674641367 0.0742814866895039 TargetScan -0.341920658891564 0.000391136109564967 NA NA NA hsa-miR-192-5p PRICKLE1 2.0867723747501 3.57854249239079e-08 -0.111521563557497 0.670884828618259 miRNAWalker2_validate -0.287669113783684 2.62804395951269e-13 NA NA NA hsa-miR-21-5p PRICKLE2 2.21515015440792 6.0468197386674e-49 -1.10520729121812 0.000108388577025068 miRNAWalker2_validate -0.608807390651799 1.54800519379245e-10 NA NA NA hsa-miR-192-5p PRKAA2 2.0867723747501 3.57854249239079e-08 -2.4618359528533 1.10256665464061e-06 miRNAWalker2_validate -0.689798430456064 5.66854629636252e-20 NA NA NA hsa-miR-21-5p PRKAB2 2.21515015440792 6.0468197386674e-49 -0.367111274928678 0.0137111089812302 miRNAWalker2_validate -0.237416078773294 1.9957106446764e-06 NA NA NA hsa-miR-200b-3p PRKACB 2.06025765178663 3.07718049337497e-10 -0.78544048113582 8.15272293615053e-05 TargetScan -0.246879516070806 1.51468418809723e-12 NA NA NA hsa-miR-192-5p PRKAR1A 2.0867723747501 3.57854249239079e-08 -0.0626576748573413 0.503137741080174 miRNAWalker2_validate -0.100024379295753 7.72049910918392e-13 NA NA NA hsa-miR-200b-3p PRKAR1A 2.06025765178663 3.07718049337497e-10 -0.0626576748573413 0.503137741080174 TargetScan -0.105267398145428 7.36823437723296e-11 NA NA NA hsa-miR-200b-3p PRKAR2B 2.06025765178663 3.07718049337497e-10 -1.74491251131701 1.12121385516986e-10 TargetScan -0.504451059563918 1.37112574474705e-28 NA NA NA hsa-miR-200b-3p PRKCA 2.06025765178663 3.07718049337497e-10 0.011989547805439 0.942502741250812 TargetScan -0.108241426571207 0.000262578924015237 NA NA NA hsa-miR-21-5p PRKCE 2.21515015440792 6.0468197386674e-49 -0.403145114198355 0.000555635541398392 miRNAWalker2_validate -0.222499913706839 1.08892726886949e-08 NA NA NA hsa-miR-192-5p PRKD3 2.0867723747501 3.57854249239079e-08 -0.32115837201335 0.0275797017891455 miRNAWalker2_validate -0.142357880982247 1.28654242154409e-10 NA NA NA hsa-miR-200b-3p PRKG1 2.06025765178663 3.07718049337497e-10 -0.561746760371328 0.0108057633457725 TargetScan -0.415373394881215 7.75966836416119e-32 NA NA NA hsa-miR-192-5p PRNP 2.0867723747501 3.57854249239079e-08 -0.769694673212605 6.60491278620587e-05 miRNAWalker2_validate -0.330216253180879 1.13124609833152e-34 NA NA NA hsa-miR-192-5p PRSS23 2.0867723747501 3.57854249239079e-08 -0.182857440489201 0.344977903609868 miRNAWalker2_validate -0.21135748848448 3.33446401420089e-13 NA NA NA hsa-miR-196a-5p PRUNE2 5.05945965885472 4.48343841468539e-25 -1.73337783953577 0.000223209263309052 miRNAWalker2_validate -0.3230115536753 1.26652016501619e-09 NA NA NA hsa-miR-200b-3p PSIP1 2.06025765178663 3.07718049337497e-10 -0.0052613598177258 0.968971847932796 TargetScan -0.134449489520673 1.35574947551006e-08 NA NA NA hsa-miR-192-5p PTBP2 2.0867723747501 3.57854249239079e-08 -0.26638936428747 0.119993496444284 miRNAWalker2_validate -0.240454028254884 2.77303443605547e-22 NA NA NA hsa-miR-18a-5p PTEN 2.04701000113742 1.30593450319289e-12 -0.187156334149252 0.0774777059361541 miRNAWalker2_validate;miRTarBase -0.100748931792874 1.71536361157568e-06 24681249;27291152 However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis;miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis metastasis; colon cancer;esophageal cancer hsa-miR-200b-3p PTGDR 2.06025765178663 3.07718049337497e-10 -0.687281185684806 0.0141390468328478 TargetScan -0.240164878138216 1.47057607004593e-06 NA NA NA hsa-miR-21-5p PTGFR 2.21515015440792 6.0468197386674e-49 -0.233414407535669 0.454209756675319 miRNAWalker2_validate -0.326004575118758 0.00201691145355239 NA NA NA hsa-miR-146b-5p PTGS1 2.23105707690238 3.83997814011313e-23 -1.86379706848248 1.06731086101655e-15 miRNAWalker2_validate -0.448232959823284 1.82874449813464e-14 NA NA NA hsa-miR-196a-5p PTGS1 5.05945965885472 4.48343841468539e-25 -1.86379706848248 1.06731086101655e-15 miRNAWalker2_validate -0.241016016825071 7.3502839197723e-20 NA NA NA hsa-miR-196b-5p PTGS1 4.51951031652328 7.04026169031626e-18 -1.86379706848248 1.06731086101655e-15 miRNAWalker2_validate -0.204596316610976 6.72526389786578e-16 NA NA NA hsa-miR-200b-3p PTHLH 2.06025765178663 3.07718049337497e-10 -0.698481509843736 0.00799227292455291 TargetScan -0.340100291189722 8.04685634951928e-14 NA NA NA hsa-miR-192-5p PTP4A3 2.0867723747501 3.57854249239079e-08 0.37089836851384 0.0714256316138787 miRNAWalker2_validate -0.139798326084273 1.18296157443871e-05 NA NA NA hsa-miR-194-5p PTPN13 2.24386881692218 9.35116329002209e-11 -1.20755288636418 4.62853401871777e-06 miRNAWalker2_validate;miRTarBase -0.447360635180318 1.51375170838027e-27 NA NA NA hsa-miR-200b-3p PTPN13 2.06025765178663 3.07718049337497e-10 -1.20755288636418 4.62853401871777e-06 TargetScan -0.433663457968814 2.2906897923458e-22 NA NA NA hsa-miR-200b-3p PTPN14 2.06025765178663 3.07718049337497e-10 0.144321960759363 0.345946539624122 TargetScan -0.25572680274256 2.93805336168299e-24 NA NA NA hsa-miR-200b-3p PTPN21 2.06025765178663 3.07718049337497e-10 -0.58065256518922 1.06468190774833e-05 TargetScan -0.177123992747243 1.07042797357042e-14 NA NA NA hsa-miR-200a-3p PTPRD 2.02394729844446 2.21240501925011e-08 -1.00502953098866 0.000637604436440412 miRNAWalker2_validate -0.271906682595162 1.09246277403848e-08 NA NA NA hsa-miR-200b-3p PTPRD 2.06025765178663 3.07718049337497e-10 -1.00502953098866 0.000637604436440412 miRNAWalker2_validate -0.288688828311511 3.45579589252849e-08 NA NA NA hsa-miR-21-5p PTX3 2.21515015440792 6.0468197386674e-49 -1.43718463297197 7.26624158154991e-07 miRNAWalker2_validate -0.680140526369636 2.07565753759716e-12 NA NA NA hsa-miR-21-5p PURA 2.21515015440792 6.0468197386674e-49 -0.330747450626133 0.000135621233435024 miRNAWalker2_validate -0.124600602874901 2.02760036867837e-05 NA NA NA hsa-miR-192-5p PXDC1 2.0867723747501 3.57854249239079e-08 -0.544516484801669 0.000990453611649091 miRNAWalker2_validate -0.113652947287408 1.05920523358882e-05 NA NA NA hsa-miR-200b-3p QKI 2.06025765178663 3.07718049337497e-10 -0.332523081792703 0.0411054129206581 TargetScan -0.290326831854982 4.74685160856375e-28 NA NA NA hsa-miR-192-5p RAB11FIP2 2.0867723747501 3.57854249239079e-08 -0.259679029578217 0.0617021736002116 miRNAWalker2_validate -0.132184387341565 3.88899214231502e-10 NA NA NA hsa-miR-200b-3p RAB11FIP2 2.06025765178663 3.07718049337497e-10 -0.259679029578217 0.0617021736002116 TargetScan -0.144162950888408 3.20530256880898e-09 NA NA NA hsa-miR-21-5p RAB11FIP2 2.21515015440792 6.0468197386674e-49 -0.259679029578217 0.0617021736002116 miRNAWalker2_validate -0.130213784529132 0.00572528163605836 NA NA NA hsa-miR-18a-5p RAB23 2.04701000113742 1.30593450319289e-12 -0.096112489735301 0.66521993302631 miRNAWalker2_validate -0.357609358549775 2.68209325911484e-17 NA NA NA hsa-miR-192-5p RAB23 2.0867723747501 3.57854249239079e-08 -0.096112489735301 0.66521993302631 miRNAWalker2_validate -0.341519884810975 1.45030597397005e-27 NA NA NA hsa-miR-200b-3p RAB23 2.06025765178663 3.07718049337497e-10 -0.096112489735301 0.66521993302631 TargetScan -0.405142952873887 8.39457399685435e-30 NA NA NA hsa-miR-200a-3p RAB30 2.02394729844446 2.21240501925011e-08 -1.42442211692912 3.65381677106471e-13 miRNAWalker2_validate -0.136257423504337 4.46555272497937e-05 NA NA NA hsa-miR-200b-3p RAB37 2.06025765178663 3.07718049337497e-10 -1.20320026232116 2.2252385940338e-06 TargetScan -0.101005535927286 0.0308450646470225 NA NA NA hsa-miR-192-5p RABGAP1 2.0867723747501 3.57854249239079e-08 -0.106752464617506 0.378462368364444 miRNAWalker2_validate -0.193308534942298 2.57444474829562e-30 NA NA NA hsa-miR-21-5p RABGAP1 2.21515015440792 6.0468197386674e-49 -0.106752464617506 0.378462368364444 miRNAWalker2_validate -0.163996670734361 5.38478686992387e-05 NA NA NA hsa-miR-192-5p RADIL 2.0867723747501 3.57854249239079e-08 -1.62758052303071 7.68103486572053e-09 miRNAWalker2_validate -0.427271623557567 8.95367396528041e-25 NA NA NA hsa-miR-200b-3p RAPGEF1 2.06025765178663 3.07718049337497e-10 -0.0152957002202374 0.878400557041415 TargetScan -0.100367470326615 8.44449291873679e-09 NA NA NA hsa-miR-200a-3p RASSF2 2.02394729844446 2.21240501925011e-08 -0.273260229164372 0.2196076417753 miRNAWalker2_validate -0.244060166615596 3.59149466603464e-12 NA NA NA hsa-miR-200b-3p RASSF2 2.06025765178663 3.07718049337497e-10 -0.273260229164372 0.2196076417753 miRNAWalker2_validate -0.252549813842515 7.110986826073e-11 NA NA NA hsa-miR-18a-5p RASSF3 2.04701000113742 1.30593450319289e-12 -0.226294581630821 0.116913602693839 miRNAWalker2_validate -0.199039300057131 1.19251429273211e-12 NA NA NA hsa-miR-192-5p RASSF8 2.0867723747501 3.57854249239079e-08 -0.121570136144733 0.655913644139023 miRNAWalker2_validate -0.376661283386746 1.63895387736148e-21 NA NA NA hsa-miR-200b-3p RASSF8 2.06025765178663 3.07718049337497e-10 -0.121570136144733 0.655913644139023 TargetScan -0.483133049279681 1.0278957185645e-27 NA NA NA hsa-miR-200b-3p RBFOX2 2.06025765178663 3.07718049337497e-10 -0.23382535811194 0.0474300848701291 TargetScan -0.236127548907516 9.17291557021335e-38 NA NA NA hsa-miR-200b-3p RBFOX3 2.06025765178663 3.07718049337497e-10 -2.19005818118938 0.000121911420336494 TargetScan -1.01713955020247 2.81387415801674e-27 NA NA NA hsa-miR-200b-3p RBM20 2.06025765178663 3.07718049337497e-10 -1.95734925558392 5.80934241681352e-07 TargetScan -0.592424506178781 1.88172738438787e-18 NA NA NA hsa-miR-192-5p RBP7 2.0867723747501 3.57854249239079e-08 -1.55108469996823 2.92968464957998e-09 miRNAWalker2_validate -0.300842249818097 9.66157548801478e-14 NA NA NA hsa-miR-192-5p RCBTB2 2.0867723747501 3.57854249239079e-08 -0.615542074181102 1.20706327268499e-05 miRNAWalker2_validate -0.203322879695303 4.82196530892446e-23 NA NA NA hsa-miR-200b-3p RECK 2.06025765178663 3.07718049337497e-10 -1.04347373160309 9.33987268915419e-07 TargetScan -0.423201877789433 5.05302593040661e-35 NA NA NA hsa-miR-21-5p RECK 2.21515015440792 6.0468197386674e-49 -1.04347373160309 9.33987268915419e-07 miRNAWalker2_validate;miRTarBase -0.462770638390227 8.85041421987572e-11 18794849;19730150;20480266;22642976;25720799 Finally we showed that RECK a known tumor suppressor in gastric cancer is a bona fide target of miR-21; Taken together miR-21 may be important in the initiation and progression of gastric cancers as an oncomiR likely through regulating RECK; Our findings suggest a potential regulatory pathway in which H pylori infection upregulates expression of miR-21 which in turn downregulates RECK and then leads to the development of gastric cancer;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;Furthermore we identified that RECK reversion-inducing-cysteine-rich protein with kazal motifs a tumor suppressor gene was a direct target of miR-21; Our results suggest that miR-21 expression has a key role in regulating cellular processes in osteosarcoma likely through regulating RECK and may serve as a therapeutic target;miR 21 may acts as an oncomir by targeting RECK a matrix metalloproteinase regulator in prostate cancer; The micro RNA miRNA mir-21 has been shown to regulate invasiveness in cancer through translational repression of the Metaloproteinase MMP inhibitor RECK; Our aim is to investigate the levels of expression of RECK and miR-21 in PCa comparing with classical prognostic factors and disease outcome and also test if RECK is a target of miR-21 in in vitro study using PCa cell line; To determine if RECK is a target of miR-21 in prostate cancer we performed an in vitro assay with PCa cell line DU-145 transfected with pre-miR-21 and anti-miR-21; To determine miR-21 and RECK expression levels in PCa samples we performed quantitative real-time polymerase chain reaction qRT-PCR; From men who presented the second profile miR-21 overexpression and RECK underexpression 91.7% were staged pT3; Our results demonstrate RECK as a target of miR-21; We believe that miR-21 may be important in PCa progression through its regulation of RECK a known regulator of tumor cell invasion;We verified that HMGB1-induced expression of miR-21 in HCC provides a posttranscriptional repression of the matrix metalloproteinase MMP inhibitors RECK and TIMP3 which are known to impact HCC progression and metastases; Finally we found that inhibition of miR-21 in murine HMGB1-overexpressing HCC xenografts led to reduced tumor MMP activity through released repression of the miR-21 targets RECK and TIMP3 which ultimately impeded tumor progression progression;;;staging;progression;metastasis;progression gastric cancer;pancreatic cancer;sarcoma;prostate cancer;liver cancer hsa-miR-192-5p REEP1 2.0867723747501 3.57854249239079e-08 -2.11544155777275 2.02942413315448e-07 miRNAWalker2_validate -0.479953402635237 1.28696497124592e-14 NA NA NA hsa-miR-200b-3p REEP1 2.06025765178663 3.07718049337497e-10 -2.11544155777275 2.02942413315448e-07 TargetScan -0.616176338172447 2.09750484334052e-18 NA NA NA hsa-miR-196a-5p REEP2 5.05945965885472 4.48343841468539e-25 -1.43591098048092 4.30296016027365e-05 miRNAWalker2_validate -0.260307038283211 5.15877706417558e-11 NA NA NA hsa-miR-200b-3p RELN 2.06025765178663 3.07718049337497e-10 -2.4032593050865 9.46041009197227e-10 TargetScan -0.634831068527533 9.16409051000065e-21 NA NA NA hsa-miR-200b-3p RERE 2.06025765178663 3.07718049337497e-10 -0.181970978434266 0.124113140521512 miRNAWalker2_validate;miRTarBase -0.115464533256617 2.66966313806461e-08 NA NA NA hsa-miR-192-5p RET 2.0867723747501 3.57854249239079e-08 -0.832755310542644 0.0351710970965439 miRNAWalker2_validate -0.280377047147025 4.90849064031775e-06 NA NA NA hsa-miR-183-5p REV3L 2.08412478942637 1.08931431254487e-10 0.0648775417046759 0.622634319982209 miRNAWalker2_validate -0.121159841563941 2.43901406939581e-07 NA NA NA hsa-miR-200b-3p RGL1 2.06025765178663 3.07718049337497e-10 -0.442559496999595 0.0118773904815059 TargetScan -0.276656234016685 6.2977174943952e-21 NA NA NA hsa-miR-21-5p RHOB 2.21515015440792 6.0468197386674e-49 -1.05159658010263 6.81750709839854e-07 miRNAWalker2_validate;miRTarBase -0.565290576123069 4.17921149096228e-16 21872591;25861727 miR 21 targets the tumor suppressor RhoB and regulates proliferation invasion and apoptosis in colorectal cancer cells; Here we show that expression of miR-21 in HEK293 and several colorectal cancer cells was found inversely correlated with ras homolog gene family member B RhoB expression; miR-21 expression significantly suppressed RhoB 3' UTR luciferase-reporter activity but the inhibitory effect was lost when the putative target sites were mutated; Exogenous miR-21 over-expression mimicked the effect of RhoB knockdown in promoting proliferation and invasion and inhibiting apoptosis whereas anti-miR-21 or RhoB expression yielded opposite effects in colorectal cancer cells; These results suggest that miR-21 is a regulator of RhoB expression and RhoB could be a useful target in exploring the potential therapeutic benefits of miR-21 mediated tumor cell behaviors in colorectal cancer;Here we demonstrate that intravenously-administered chlorotoxin CTX-coupled targeted stable nucleic acid lipid particle SNALP-formulated anti-miR-21 oligonucleotides accumulate preferentially within brain tumors and promote efficient miR-21 silencing which results in increased mRNA and protein levels of its target RhoB while showing no signs of systemic immunogenicity ; colorectal cancer;glioblastoma hsa-miR-192-5p RHOBTB3 2.0867723747501 3.57854249239079e-08 0.116753499077459 0.613081108966582 miRNAWalker2_validate -0.194985894264289 3.46829160374809e-08 NA NA NA hsa-miR-21-5p RHOQ 2.21515015440792 6.0468197386674e-49 -0.181509167275028 0.112005666549592 miRNAWalker2_validate -0.158846177299076 3.32012120234618e-05 NA NA NA hsa-miR-200b-3p RIMKLB 2.06025765178663 3.07718049337497e-10 -0.516647470958743 0.0376812451743227 TargetScan -0.366519023218365 3.08828017106888e-18 NA NA NA hsa-miR-200b-3p RIMS2 2.06025765178663 3.07718049337497e-10 -1.36522903123664 0.00207602001734613 TargetScan -0.57810708470783 5.29128162742176e-14 NA NA NA hsa-miR-200b-3p RIMS3 2.06025765178663 3.07718049337497e-10 -1.3046164844765 2.73366807369862e-06 TargetScan -0.417630904651072 2.75647444951043e-18 NA NA NA hsa-miR-200b-3p RNF11 2.06025765178663 3.07718049337497e-10 -0.739445158915551 3.42257608103258e-15 TargetScan -0.143407154989315 8.89340074425969e-18 NA NA NA hsa-miR-21-5p RNF11 2.21515015440792 6.0468197386674e-49 -0.739445158915551 3.42257608103258e-15 miRNAWalker2_validate -0.259757279535172 2.45270849985509e-16 NA NA NA hsa-miR-200b-3p RNF38 2.06025765178663 3.07718049337497e-10 -0.173471812012056 0.142869431269605 TargetScan -0.163894852040884 3.4644896189701e-16 NA NA NA hsa-miR-200b-3p RPS6KA2 2.06025765178663 3.07718049337497e-10 -0.569696503739038 0.00012691212615164 TargetScan -0.183081097691008 1.82222094894106e-12 NA NA NA hsa-miR-183-5p RTN4 2.08412478942637 1.08931431254487e-10 -0.411549538148011 0.000141083434427497 miRNAWalker2_validate -0.146520538083903 8.33727478172824e-15 NA NA NA hsa-miR-21-5p RTN4 2.21515015440792 6.0468197386674e-49 -0.411549538148011 0.000141083434427497 miRNAWalker2_validate;miRTarBase -0.161265147044833 1.02187754265933e-05 NA NA NA hsa-miR-192-5p RUFY3 2.0867723747501 3.57854249239079e-08 -0.289478272206307 0.00359568710719357 miRNAWalker2_validate -0.125581649670283 1.06502779180138e-17 NA NA NA hsa-miR-200b-3p RUNX1T1 2.06025765178663 3.07718049337497e-10 -0.344000642439472 0.237404358600589 TargetScan -0.498780478272802 1.44553040827209e-25 NA NA NA hsa-miR-200b-3p RUSC2 2.06025765178663 3.07718049337497e-10 -0.140088157149797 0.375609388037744 TargetScan -0.277382036667665 3.37739602544384e-27 NA NA NA hsa-miR-21-5p SASH1 2.21515015440792 6.0468197386674e-49 -0.502378415947332 0.00175474680872799 miRNAWalker2_validate -0.275252945138833 3.23373732718039e-07 NA NA NA hsa-miR-200b-3p SBSPON 2.06025765178663 3.07718049337497e-10 -1.82449074362515 5.14488465520048e-07 TargetScan -0.48490473573115 3.02227370466804e-14 NA NA NA hsa-miR-192-5p SCMH1 2.0867723747501 3.57854249239079e-08 -0.43649737377921 0.00104525100356383 miRNAWalker2_validate -0.152157520902981 2.9742996207332e-14 NA NA NA hsa-miR-196a-5p SCN11A 5.05945965885472 4.48343841468539e-25 -1.14952987855662 0.00299169454930274 miRNAWalker2_validate -0.198519305086489 7.62685431522637e-06 NA NA NA hsa-miR-200b-3p SCN2A 2.06025765178663 3.07718049337497e-10 0.317164447705599 0.477497388671926 TargetScan -0.302181872769901 0.00015262490405479 NA NA NA hsa-miR-200b-3p SCN3B 2.06025765178663 3.07718049337497e-10 -1.12424455507364 0.000589168065844479 TargetScan -0.456910237126413 4.94035125738474e-16 NA NA NA hsa-miR-200b-3p SCN5A 2.06025765178663 3.07718049337497e-10 -0.259448692335781 0.48224277680017 TargetScan -0.289726916033095 1.02081984009576e-05 NA NA NA hsa-miR-21-5p SCRN1 2.21515015440792 6.0468197386674e-49 -0.00151151687256945 0.995131134492659 miRNAWalker2_validate -0.174031609110733 0.0387270087024981 NA NA NA hsa-miR-200b-3p SDC2 2.06025765178663 3.07718049337497e-10 -0.361794289426367 0.0421687423135213 TargetScan -0.293280882513722 2.65649850544273e-23 NA NA NA hsa-miR-200b-3p SDK2 2.06025765178663 3.07718049337497e-10 -0.581686295778627 0.0604550673556843 TargetScan -0.128843433487224 0.0213740112033626 NA NA NA hsa-miR-200b-3p SEC23A 2.06025765178663 3.07718049337497e-10 -0.428530407481632 0.000517364444097623 TargetScan -0.20657309547581 6.99921830187599e-24 NA NA NA hsa-miR-200b-3p SECISBP2L 2.06025765178663 3.07718049337497e-10 -0.57031166178403 4.09953159416214e-08 TargetScan -0.14712915810987 4.26992791693372e-16 NA NA NA hsa-miR-200b-3p SEMA6D 2.06025765178663 3.07718049337497e-10 -1.55565558119296 1.29394597397564e-07 TargetScan -0.281805749940666 1.25498679278817e-07 NA NA NA hsa-miR-192-5p SEPT10 2.0867723747501 3.57854249239079e-08 -0.29436849945031 0.0169826283606985 miRNAWalker2_validate -0.143561757199142 5.83373079076226e-15 NA NA NA hsa-miR-200a-3p SEPT7 2.02394729844446 2.21240501925011e-08 0.0277761378075443 0.749990388336138 miRNAWalker2_validate -0.124476266869426 2.19101554132703e-21 NA NA NA hsa-miR-200b-3p SEPT7 2.06025765178663 3.07718049337497e-10 0.0277761378075443 0.749990388336138 miRNAWalker2_validate -0.129857380114629 3.84748058014405e-19 NA NA NA hsa-miR-200b-3p SERINC1 2.06025765178663 3.07718049337497e-10 -0.381876612143236 3.23474497421462e-05 TargetScan -0.15147733842841 5.06226539143008e-23 NA NA NA hsa-miR-200b-3p SERPINI1 2.06025765178663 3.07718049337497e-10 -0.404315037266264 0.019875159578311 TargetScan -0.135558820730434 1.20381248680509e-05 NA NA NA hsa-miR-21-5p SERPINI1 2.21515015440792 6.0468197386674e-49 -0.404315037266264 0.019875159578311 miRNAWalker2_validate -0.188007539077205 0.00141707610411579 22464652 MicroRNA 21 inhibits Serpini1 a gene with novel tumour suppressive effects in gastric cancer; Molecular techniques include microRNA microarrays cDNA microarrays qRT-PCR for miR and mRNA expression transfection of MKN28 with miR-21 inhibitor or Serpini1 followed by Western blotting cell cycle analysis by flow cytometry and luciferase reporter assay; This search identified Serpini1 as a putative miR-21 target; Luciferase assays demonstrated direct interaction between miR-21 and Serpini1 3'UTR miR-21 and Serpini1 expression levels were inversely correlated in a subgroup of gastric cancers suggesting a regulatory mechanism that included both of these molecules; Taken together these data suggest that in a subgroup of gastric cancers miR-21 is upregulated inducing downregulation of Serpini1 which in turn releases the G1-S transition checkpoint with the end result being increased tumour growth gastric cancer hsa-miR-183-5p SERTAD4 2.08412478942637 1.08931431254487e-10 -0.855300469182066 0.00851964081929357 miRNAWalker2_validate -0.509937530817699 3.4909589123251e-20 NA NA NA hsa-miR-192-5p SERTAD4 2.0867723747501 3.57854249239079e-08 -0.855300469182066 0.00851964081929357 miRNAWalker2_validate -0.389487054289046 1.08051364705783e-15 NA NA NA hsa-miR-21-5p SESN1 2.21515015440792 6.0468197386674e-49 -0.316273897424733 0.0791103584277436 miRNAWalker2_validate -0.177386141100824 0.00369222639606522 NA NA NA hsa-miR-200b-3p SESN3 2.06025765178663 3.07718049337497e-10 -0.63123898051413 0.0145175326583435 TargetScan -0.224407240713081 1.04086423098693e-06 NA NA NA hsa-miR-200b-3p SETD7 2.06025765178663 3.07718049337497e-10 -0.28906268449287 0.0651104635619279 TargetScan -0.215063867888677 8.64274226154656e-16 NA NA NA hsa-miR-192-5p SGCB 2.0867723747501 3.57854249239079e-08 -0.278110445030599 0.0486812755185084 miRNAWalker2_validate -0.146200769812761 6.57053487211193e-12 NA NA NA hsa-miR-21-5p SGCB 2.21515015440792 6.0468197386674e-49 -0.278110445030599 0.0486812755185084 miRNAWalker2_validate -0.187935917103744 7.59518628554128e-05 NA NA NA hsa-miR-200b-3p SGCE 2.06025765178663 3.07718049337497e-10 -0.887861455136679 0.000291735874774958 TargetScan -0.452427921680518 9.16325755973155e-30 NA NA NA hsa-miR-200b-3p SGIP1 2.06025765178663 3.07718049337497e-10 0.300220569989602 0.133137161334211 TargetScan -0.267656804124419 5.1286550669651e-15 NA NA NA hsa-miR-183-5p SH3D19 2.08412478942637 1.08931431254487e-10 -0.40589349119468 0.00593283404485438 miRNAWalker2_validate -0.165887396829302 2.1849658839124e-10 NA NA NA hsa-miR-200b-3p SH3PXD2A 2.06025765178663 3.07718049337497e-10 0.104519604538216 0.49534024563541 TargetScan -0.153850441505416 1.00664247564097e-08 NA NA NA hsa-miR-183-5p SHISA2 2.08412478942637 1.08931431254487e-10 1.11799062553653 0.000261186958590419 miRNAWalker2_validate -0.111665912515352 0.0484229386147987 NA NA NA hsa-miR-200b-3p SHROOM4 2.06025765178663 3.07718049337497e-10 0.533433249282303 0.0033357310845142 TargetScan -0.137284559544724 2.49483560510638e-05 NA NA NA hsa-miR-200b-3p SIK2 2.06025765178663 3.07718049337497e-10 -0.498358334486584 1.42722402092494e-05 TargetScan -0.111935095693208 4.59557286723215e-08 NA NA NA hsa-miR-21-5p SLAIN2 2.21515015440792 6.0468197386674e-49 -0.450230709640584 7.71615685568148e-08 miRNAWalker2_validate -0.1681255879034 2.38512698180717e-09 NA NA NA hsa-miR-200b-3p SLC14A1 2.06025765178663 3.07718049337497e-10 -0.774209953172634 0.00506651647534284 TargetScan -0.185002178087437 0.00019772588099434 NA NA NA hsa-miR-192-5p SLC16A14 2.0867723747501 3.57854249239079e-08 -0.128026574623716 0.572732236984336 miRNAWalker2_validate -0.146965368157999 2.94418251492928e-05 NA NA NA hsa-miR-200b-3p SLC16A2 2.06025765178663 3.07718049337497e-10 -0.392210629327451 0.126440512346252 TargetScan -0.217616487329425 1.77166449938593e-06 NA NA NA hsa-miR-192-5p SLC16A7 2.0867723747501 3.57854249239079e-08 -1.78740503823139 7.82439110480803e-09 miRNAWalker2_validate -0.297492890773225 9.3570187164918e-10 NA NA NA hsa-miR-194-5p SLC16A9 2.24386881692218 9.35116329002209e-11 -2.32499767094881 1.72575242459783e-07 miRNAWalker2_validate -0.185347791106115 0.0167098991208726 NA NA NA hsa-miR-200b-3p SLC23A2 2.06025765178663 3.07718049337497e-10 -0.0699749852409353 0.607901925455689 TargetScan -0.130961406035207 4.43770228324087e-08 NA NA NA hsa-miR-200b-3p SLC24A4 2.06025765178663 3.07718049337497e-10 -0.889410656770861 9.42341078455181e-05 TargetScan -0.307811828843244 6.47994611572205e-15 NA NA NA hsa-miR-200b-3p SLC2A3 2.06025765178663 3.07718049337497e-10 0.21944182973729 0.225968938814907 TargetScan -0.112911156544842 0.000498117825800863 NA NA NA hsa-miR-92a-1-5p SLC2A4 2.04820501765632 9.32117486799495e-13 -2.41796075460274 1.09847746343808e-09 miRNAWalker2_validate -0.730026598510965 3.16746172755736e-20 NA NA NA hsa-miR-192-5p SLC35F3 2.0867723747501 3.57854249239079e-08 -1.63319200376934 4.64079893828067e-05 miRNAWalker2_validate -0.23513436706983 0.000207434956310952 NA NA NA hsa-miR-192-5p SLC48A1 2.0867723747501 3.57854249239079e-08 -0.514682081934797 3.48999885269257e-07 miRNAWalker2_validate -0.135810540441527 1.66974940976454e-19 NA NA NA hsa-miR-200b-3p SLC6A1 2.06025765178663 3.07718049337497e-10 0.994406984622405 9.59593686334557e-05 TargetScan -0.167134338095253 0.000299100429838722 NA NA NA hsa-miR-200b-3p SLC6A11 2.06025765178663 3.07718049337497e-10 -0.837595880554685 0.0996275345445822 TargetScan -0.211845907785713 0.0211400529635181 NA NA NA hsa-miR-196b-5p SLC6A15 4.51951031652328 7.04026169031626e-18 -2.37276785545784 1.70421464548129e-05 miRNAWalker2_validate -0.306243714763014 3.0876218376812e-07 NA NA NA hsa-miR-200b-3p SLC6A17 2.06025765178663 3.07718049337497e-10 -0.923464521615343 0.00716410237201486 TargetScan -0.399426550851497 2.89430991681926e-11 NA NA NA hsa-miR-192-5p SLC7A2 2.0867723747501 3.57854249239079e-08 -0.504055476896812 0.159045414836461 miRNAWalker2_validate -0.29980255271074 4.91686651391359e-08 NA NA NA hsa-miR-192-5p SLCO3A1 2.0867723747501 3.57854249239079e-08 0.704723025360271 0.000172885275405704 miRNAWalker2_validate -0.137933155360781 2.53321412148695e-06 NA NA NA hsa-miR-200b-3p SLIT2 2.06025765178663 3.07718049337497e-10 -1.0749206484349 0.0038634611143328 TargetScan -0.675850836377661 6.79363483823133e-29 NA NA NA hsa-miR-21-5p SLMAP 2.21515015440792 6.0468197386674e-49 -0.731049756779606 4.09382011272657e-05 miRNAWalker2_validate -0.374641831854935 2.97310314620869e-10 NA NA NA hsa-miR-18a-5p SMAD4 2.04701000113742 1.30593450319289e-12 -0.31259096016241 0.0041260218763756 miRNAWalker2_validate -0.102223897399558 2.68354883367347e-06 NA NA NA hsa-miR-200b-3p SMAD9 2.06025765178663 3.07718049337497e-10 -1.3017262581652 4.19826165756804e-05 TargetScan -0.440772198294869 1.17201241753696e-15 NA NA NA hsa-miR-192-5p SMARCA2 2.0867723747501 3.57854249239079e-08 -0.528624116298568 0.000138860805442413 miRNAWalker2_validate -0.145188705679969 6.6385021458089e-12 NA NA NA hsa-miR-200b-3p SNAI2 2.06025765178663 3.07718049337497e-10 -0.0530603959694083 0.773251196834074 TargetScan -0.165498559433967 3.49597886475489e-07 NA NA NA hsa-miR-200b-3p SNAP25 2.06025765178663 3.07718049337497e-10 -1.88036551123256 0.000140837414790177 TargetScan -0.743716900905436 1.19213621979677e-18 NA NA NA hsa-miR-21-5p SNRK 2.21515015440792 6.0468197386674e-49 -0.440363445705636 2.69716836697876e-05 miRNAWalker2_validate -0.20137262202129 9.70139742757545e-09 NA NA NA hsa-miR-194-5p SOCS2 2.24386881692218 9.35116329002209e-11 -1.10044523318765 6.64329026112842e-10 miRNAWalker2_validate;miRTarBase -0.14746253020015 1.6672323768358e-06 22102710 Functional studies indicated that CDKN1A/p21 and SOCS2 expression is directly regulated by miR-20a/-20b and miR-194 respectively; miR-20a/b and miR-194 target CDKN1A and SOCS2 in follicular lymphoma potentially contributing to tumor cell proliferation and survival poor survival lymphoma hsa-miR-21-5p SOD3 2.21515015440792 6.0468197386674e-49 -0.930102971360054 0.000795497639706759 miRNAWalker2_validate;miRTarBase -0.537791284895313 6.51564542517929e-09 NA NA NA hsa-miR-21-5p SOX2 2.21515015440792 6.0468197386674e-49 -2.14859025311565 0.00035012271767378 miRNAWalker2_validate -0.729903923617438 0.000377917304316825 26558781 Mir 21 Sox2 Axis Delineates Glioblastoma Subtypes with Prognostic Impact; Here we report that the stemness regulator Sox2 is a new clinically important target of microRNA-21 miR-21 in GBM with implications for prognosis; Further analysis of the miR-21-Sox2 relationship in mouse neural stem cells and in the mouse brain at different developmental stages indicates that miR-21 and Sox2 are predominantly expressed in mutually exclusive patterns suggesting a role in normal neural development worse prognosis;staging glioblastoma hsa-miR-194-5p SOX5 2.24386881692218 9.35116329002209e-11 -1.09083873380211 3.82011335261816e-05 miRNAWalker2_validate;miRTarBase -0.305658128044367 3.1724639015086e-12 NA NA NA hsa-miR-200b-3p SOX5 2.06025765178663 3.07718049337497e-10 -1.09083873380211 3.82011335261816e-05 TargetScan -0.38516161071944 2.87477272822889e-17 NA NA NA hsa-miR-21-5p SOX5 2.21515015440792 6.0468197386674e-49 -1.09083873380211 3.82011335261816e-05 miRNAWalker2_validate;miRTarBase -0.588926984125464 2.30670444869962e-11 NA NA NA hsa-miR-200b-3p SPG20 2.06025765178663 3.07718049337497e-10 -1.16044309087638 2.11945718235849e-07 TargetScan -0.396748392650767 2.91636381754576e-26 NA NA NA hsa-miR-200a-3p SRF 2.02394729844446 2.21240501925011e-08 -0.373426314355344 0.00505633063985255 miRTarBase -0.218918496517429 1.08016740824401e-28 NA NA NA hsa-miR-200b-3p SRF 2.06025765178663 3.07718049337497e-10 -0.373426314355344 0.00505633063985255 TargetScan -0.235037143254377 3.27007577422506e-27 NA NA NA hsa-miR-200b-3p ST3GAL2 2.06025765178663 3.07718049337497e-10 0.591994265703637 8.60761825288054e-05 TargetScan -0.143306026062821 9.88437838653042e-08 NA NA NA hsa-miR-192-5p ST3GAL6 2.0867723747501 3.57854249239079e-08 -0.515506786199837 0.00194391260076259 miRNAWalker2_validate -0.135789966475308 1.30524303338148e-07 NA NA NA hsa-miR-200b-3p ST6GALNAC5 2.06025765178663 3.07718049337497e-10 0.0593929043190893 0.834153304191114 TargetScan -0.44867718848151 2.09270591228023e-21 NA NA NA hsa-miR-200b-3p ST8SIA3 2.06025765178663 3.07718049337497e-10 -2.91558860135036 1.52609950297091e-08 TargetScan -0.623937282615249 1.2441486038807e-11 NA NA NA hsa-miR-200b-3p STARD13 2.06025765178663 3.07718049337497e-10 -0.186544413090553 0.273832535954335 TargetScan -0.251061223273788 2.33275856056792e-18 NA NA NA hsa-miR-183-5p STK33 2.08412478942637 1.08931431254487e-10 -0.92864452301099 0.00657026101364563 miRNAWalker2_validate -0.431959264836519 6.26293851149042e-13 NA NA NA hsa-miR-183-5p STK40 2.08412478942637 1.08931431254487e-10 -0.426530912271271 3.52828839037143e-05 miRNAWalker2_validate -0.108222462361264 4.67344977345167e-09 NA NA NA hsa-miR-192-5p STOX2 2.0867723747501 3.57854249239079e-08 -1.38885793271292 7.68761182671221e-06 miRNAWalker2_validate -0.297829809837207 5.92017630551452e-10 NA NA NA hsa-miR-200b-3p STRN3 2.06025765178663 3.07718049337497e-10 -0.14407563641855 0.145314550144892 TargetScan -0.10685916064434 5.08670870550869e-10 NA NA NA hsa-miR-18a-5p STX12 2.04701000113742 1.30593450319289e-12 -0.882181299570957 2.03175763589976e-22 miRNAWalker2_validate -0.118484104043417 7.95805486812409e-10 NA NA NA hsa-miR-200b-3p STXBP6 2.06025765178663 3.07718049337497e-10 -0.727299176015706 0.011776569506795 TargetScan -0.149570400110826 0.00414185544386918 NA NA NA hsa-miR-200b-3p SULF1 2.06025765178663 3.07718049337497e-10 2.66492104637834 5.21304611366659e-20 TargetScan -0.138332768869932 0.0144696325136573 NA NA NA hsa-miR-200b-3p SYDE1 2.06025765178663 3.07718049337497e-10 -0.0640700013959519 0.734826242563291 TargetScan -0.362658958637974 8.6322888920495e-34 NA NA NA hsa-miR-200b-3p SYT1 2.06025765178663 3.07718049337497e-10 -0.131809945129705 0.773109960771398 TargetScan -0.445400906687112 3.06992235440953e-08 NA NA NA hsa-miR-196a-5p SYT9 5.05945965885472 4.48343841468539e-25 -1.2424741563645 0.001403286587921 miRNAWalker2_validate -0.234504805978341 1.18827257418339e-07 NA NA NA hsa-miR-192-5p TAF9B 2.0867723747501 3.57854249239079e-08 -0.215431801968458 0.0920309395574713 miRNAWalker2_validate -0.174513080596497 5.10925335435812e-21 NA NA NA hsa-miR-200b-3p TBCEL 2.06025765178663 3.07718049337497e-10 -0.400003822040212 0.0189360861907399 TargetScan -0.194588443645355 6.03451290512809e-11 NA NA NA hsa-miR-192-5p TBL1X 2.0867723747501 3.57854249239079e-08 -0.252702013629877 0.178914931206428 miRNAWalker2_validate -0.210131363753877 8.16390693657987e-14 NA NA NA hsa-miR-200b-3p TBX5 2.06025765178663 3.07718049337497e-10 0.960968095527673 0.10661691842954 TargetScan -0.246857912942069 0.0217606332629862 NA NA NA hsa-miR-18a-5p TCEA2 2.04701000113742 1.30593450319289e-12 -0.653060587847677 0.00171167383430329 miRNAWalker2_validate -0.247641417809946 2.05533988786389e-09 NA NA NA hsa-miR-21-5p TCF21 2.21515015440792 6.0468197386674e-49 -1.32513315450316 2.22849997566556e-08 miRNAWalker2_validate -0.552529711637857 3.81809759900787e-12 NA NA NA hsa-miR-200b-3p TCF4 2.06025765178663 3.07718049337497e-10 0.0159386247034305 0.922706223016712 TargetScan -0.225255072605693 7.35257467511235e-16 NA NA NA hsa-miR-200a-3p TCF7L1 2.02394729844446 2.21240501925011e-08 -0.950810170611323 2.06235136278501e-05 miRNAWalker2_validate -0.405182514690993 1.2373922056801e-35 NA NA NA hsa-miR-200b-3p TCF7L1 2.06025765178663 3.07718049337497e-10 -0.950810170611323 2.06235136278501e-05 miRNAWalker2_validate -0.442824188097337 3.15038718766065e-35 NA NA NA hsa-miR-200b-3p TEAD1 2.06025765178663 3.07718049337497e-10 -0.180963993433754 0.334001704599182 TargetScan -0.32760980527879 6.12847459243077e-27 NA NA NA hsa-miR-21-5p TESK2 2.21515015440792 6.0468197386674e-49 -0.789206095132177 9.25451549840534e-12 miRNAWalker2_validate -0.218555409375617 4.50003818642008e-08 NA NA NA hsa-miR-18a-5p TGFBR2 2.04701000113742 1.30593450319289e-12 -0.243315633013612 0.114081643080486 miRNAWalker2_validate -0.209546376706227 2.56963197608545e-12 NA NA NA hsa-miR-21-5p TGFBR3 2.21515015440792 6.0468197386674e-49 -1.17329353105567 5.76332601597347e-06 miRNAWalker2_validate -0.58929783986775 7.9673565096856e-12 NA NA NA hsa-miR-192-5p THBD 2.0867723747501 3.57854249239079e-08 -0.791451046098264 5.07141412103183e-05 miRNAWalker2_validate -0.192372195439883 1.53383572386925e-10 NA NA NA hsa-miR-200b-3p THRB 2.06025765178663 3.07718049337497e-10 -1.11745660181354 0.00039648730743052 TargetScan -0.365906730083304 4.19911057757576e-11 NA NA NA hsa-miR-200b-3p THSD7A 2.06025765178663 3.07718049337497e-10 0.242279573672174 0.251540034596406 TargetScan -0.272474534273393 6.06531100434947e-14 NA NA NA hsa-miR-21-5p TIAM1 2.21515015440792 6.0468197386674e-49 -0.741051913482461 0.00185201928858849 miRNAWalker2_validate;miRTarBase -0.291116901444628 0.000324644941290984 20826792 miR 21 and miR 31 converge on TIAM1 to regulate migration and invasion of colon carcinoma cells; We present compelling evidence that TIAM1 a guanidine exchange factor of the Rac GTPase is a direct target of both miR-21 and miR-31 colon cancer hsa-miR-200b-3p TIMP2 2.06025765178663 3.07718049337497e-10 -0.112188449077383 0.558631566061868 TargetScan -0.339861164647159 8.22085239291579e-28 23205572 A novel target of miR-200b tissue inhibitor of metalloproteinase 2 TIMP2 was predicted using a bioinformatics approach and was confirmed in human endometrial cancer cell line HEC-1A cells by luciferase assay quantitative real-time polymerase chain reaction western blotting and enzyme-linked immunosorbent assay; We found that miR-200b repressed TIMP2 expression at both the messenger RNA and protein levels although a family member miR-200a had no such effect; Using reverse gelatin zymography we showed that miR-200b enhances matrix metallopeptidase 2 MMP2 activity by downregulating TIMP2 expression in HEC-1A cells endometrial cancer hsa-miR-21-5p TIMP3 2.21515015440792 6.0468197386674e-49 -0.545766258963537 0.0230696906023966 miRNAWalker2_validate;miRTarBase -0.34367964487154 2.19174550707526e-05 23936642;21820586;25720799;20346171;25587717 Genetic heterogeneity of breast cancer metastasis may be related to miR 21 regulation of TIMP 3 in translation; The aim of this study is to determine whether microRNA-21 miR-21 a specific microRNA implicated in multiple aspects of carcinogenesis promoted breast cancer metastasis by regulating the tissue inhibitor of metalloproteinase 3 TIMP-3 gene; Correlation analysis was performed between miR-21 and TIMP-3; There was significantly inverse correlation between miR-21 and TIMP-3 extracted from tissue;The effect of TIMP3 on miR-21-induced cell survival was determined by transfection with TIMP3 lacking 3' untranslated region and miR-21;We verified that HMGB1-induced expression of miR-21 in HCC provides a posttranscriptional repression of the matrix metalloproteinase MMP inhibitors RECK and TIMP3 which are known to impact HCC progression and metastases; Finally we found that inhibition of miR-21 in murine HMGB1-overexpressing HCC xenografts led to reduced tumor MMP activity through released repression of the miR-21 targets RECK and TIMP3 which ultimately impeded tumor progression;The aim of this study was to determine whether microRNA-21 miR-21 a specific microRNA implicated in multiple aspects of carcinogenesis impacts breast cancer invasion by regulating the tissue inhibitor of metalloproteinase 3 TIMP3 gene; In addition the regulation of tissue inhibitor of metalloproteinase 3 TIMP3 by miR-21 was evaluated by western blotting and luciferase assays; Evaluation of TIMP3 protein levels a peptidase involved in extarcellular matrix degredation inversely correlated with miR-21 expression; As knockdown of miR-21 increased TIMP3 protein expression and luciferase reporter activity our data suggests that miR-21 could promote invasion in breast cancer cells via its regulation of TIMP3;It was previously shown by our group that miR-21 a potential regulator of TIMP3 is over-expressed in cutaneous melanoma; It was therefore hypothesized that increased levels of miR-21 expression would lead to decreased expression of TIMP3 and thereby enhance the invasiveness of melanoma cells; Reduced expression of TIMP3 was achieved by siRNA knockdown and significantly enhanced invasion of melanoma cell lines mimicking the effects of miR-21 over-expression; Treatment of tumor cells with a linked nucleic acid antagomir to miR-21 inhibited tumor growth and increased tumor expression of TIMP3 in vivo in 01B74 Athymic NCr-nu/nu mice; This data shows that increased expression of miR-21 enhanced the invasive potential of melanoma cell lines through TIMP3 inhibition metastasis;tumorigenesis;poor survival;metastasis;progression;tumorigenesis; breast cancer;kidney renal cell cancer;liver cancer;breast cancer;melanoma hsa-miR-200b-3p TJP1 2.06025765178663 3.07718049337497e-10 -0.131938594922226 0.315131821076645 TargetScan -0.105051726159028 6.31057595486219e-06 NA NA NA hsa-miR-200b-3p TLN1 2.06025765178663 3.07718049337497e-10 -0.287038594162333 0.0813060668186822 TargetScan -0.329488776067562 1.50804597491702e-37 NA NA NA hsa-miR-200b-3p TMEFF2 2.06025765178663 3.07718049337497e-10 -2.42593068961104 8.20730991998178e-08 TargetScan -0.666358842419847 2.58075584135151e-17 NA NA NA hsa-miR-183-5p TMEM108 2.08412478942637 1.08931431254487e-10 -1.3113142499809 1.81979452471885e-09 miRNAWalker2_validate -0.391096569710113 1.06744311780889e-25 NA NA NA hsa-miR-192-5p TMEM136 2.0867723747501 3.57854249239079e-08 -0.269780737415521 0.169447074380575 miRNAWalker2_validate -0.20956477160737 1.30549229147536e-12 NA NA NA hsa-miR-200b-3p TMEM136 2.06025765178663 3.07718049337497e-10 -0.269780737415521 0.169447074380575 TargetScan -0.273342489646391 2.82986643677767e-16 NA NA NA hsa-miR-192-5p TMEM169 2.0867723747501 3.57854249239079e-08 0.160305852802737 0.4754028858431 miRNAWalker2_validate -0.123841264547483 0.00039914015236008 NA NA NA hsa-miR-192-5p TMEM17 2.0867723747501 3.57854249239079e-08 0.120835414929376 0.517397662876049 miRNAWalker2_validate -0.131853583471726 4.75087075893918e-06 NA NA NA hsa-miR-200b-3p TMEM17 2.06025765178663 3.07718049337497e-10 0.120835414929376 0.517397662876049 TargetScan -0.120821757628461 0.000289999575811601 NA NA NA hsa-miR-200b-3p TMEM170B 2.06025765178663 3.07718049337497e-10 -0.410928074487889 0.068480801438453 TargetScan -0.307541913896212 1.5465227988557e-15 NA NA NA hsa-miR-183-5p TMEM245 2.08412478942637 1.08931431254487e-10 -0.242721302151875 0.0930172579576728 miRNAWalker2_validate -0.233424403194951 7.55068175107412e-22 NA NA NA hsa-miR-200b-3p TMEM245 2.06025765178663 3.07718049337497e-10 -0.242721302151875 0.0930172579576728 TargetScan -0.232632184250286 2.9519181237607e-22 NA NA NA hsa-miR-21-5p TMEM245 2.21515015440792 6.0468197386674e-49 -0.242721302151875 0.0930172579576728 miRNAWalker2_validate -0.218389171822637 6.40278187413313e-06 NA NA NA hsa-miR-21-5p TMEM56 2.21515015440792 6.0468197386674e-49 -0.651275261976962 0.00109328650423537 miRNAWalker2_validate -0.224438512352995 0.000966225188268164 NA NA NA hsa-miR-200b-3p TMX4 2.06025765178663 3.07718049337497e-10 -0.330403187240345 0.0125813746541551 TargetScan -0.125218916229639 8.59396680856445e-08 NA NA NA hsa-miR-21-5p TMX4 2.21515015440792 6.0468197386674e-49 -0.330403187240345 0.0125813746541551 miRNAWalker2_validate -0.192227592579899 1.58300156345407e-05 NA NA NA hsa-miR-183-5p TNRC6B 2.08412478942637 1.08931431254487e-10 0.0200605311980917 0.853993906874431 miRNAWalker2_validate -0.114336247742014 2.63802632721441e-09 NA NA NA hsa-miR-192-5p TOR1AIP1 2.0867723747501 3.57854249239079e-08 -0.562316478900568 1.82898321931949e-07 miRNAWalker2_validate -0.18618085379582 7.04519235540636e-35 NA NA NA hsa-miR-200b-3p TOR1AIP1 2.06025765178663 3.07718049337497e-10 -0.562316478900568 1.82898321931949e-07 TargetScan -0.202239007384029 2.49477975505636e-30 NA NA NA hsa-miR-21-5p TPM1 2.21515015440792 6.0468197386674e-49 -1.25535971958143 8.44875947902356e-08 miRNAWalker2_validate;miRTarBase -0.685577834600619 2.31498153951898e-19 20952761;23558936 These two miRNAs have previously been identified as being overexpressed in MCF-7 breast cancer cells with miR-21 specifically implicated in down-regulating the tumor suppressor gene tropomyosin-1;Furthermore miR-21 mimic or inhibitor significantly reduced or increased the expression of PDCD4 and TPM1; MiR-21 is overexpressed in RCC tissue and modulates the growth apoptosis and cell cycle progression of RCC cells and regulates the expression of PDCD4 and TPM1 ;progression breast cancer;kidney renal cell cancer hsa-miR-21-5p TPRG1L 2.21515015440792 6.0468197386674e-49 -0.616973838753045 5.38924149646892e-12 miRNAWalker2_validate -0.226881348775698 4.34333978320703e-14 NA NA NA hsa-miR-192-5p TRIM23 2.0867723747501 3.57854249239079e-08 -0.342721432687234 0.00211580921154758 miRNAWalker2_validate -0.172431252443368 1.21328425432394e-27 NA NA NA hsa-miR-200b-3p TRIO 2.06025765178663 3.07718049337497e-10 0.709506263927999 2.17818099879629e-06 TargetScan -0.13718873323502 3.61354594636322e-07 NA NA NA hsa-miR-183-5p TRO 2.08412478942637 1.08931431254487e-10 -0.148177017444581 0.473781590601723 miRNAWalker2_validate -0.307530651759931 2.89315441153815e-18 NA NA NA hsa-miR-192-5p TRPC1 2.0867723747501 3.57854249239079e-08 -0.704677053980514 0.00534368864806038 miRNAWalker2_validate -0.389586869598043 3.50668563206409e-27 NA NA NA hsa-miR-200b-3p TSC22D1 2.06025765178663 3.07718049337497e-10 -0.528505038934999 1.98487242401898e-05 TargetScan -0.149233700643198 6.67395833868741e-12 NA NA NA hsa-miR-200b-3p TSC22D2 2.06025765178663 3.07718049337497e-10 -0.442389045444121 7.38597549150903e-05 TargetScan -0.100591969139521 4.41857168619621e-07 NA NA NA hsa-miR-18a-5p TSC22D3 2.04701000113742 1.30593450319289e-12 -1.74603210320545 3.61843236827956e-15 miRNAWalker2_validate -0.418370912316003 1.81472950101773e-21 NA NA NA hsa-miR-21-5p TSHZ3 2.21515015440792 6.0468197386674e-49 -0.556373800983045 0.0151607966428605 miRNAWalker2_validate -0.406515247243031 1.09107311064047e-07 NA NA NA hsa-miR-200b-3p TUBB3 2.06025765178663 3.07718049337497e-10 1.70550558746553 8.12639200368567e-12 TargetScan -0.114227404230901 0.014840997415908 NA NA NA hsa-miR-18a-5p TXNDC15 2.04701000113742 1.30593450319289e-12 -0.541269491654419 1.44216720811775e-08 miRNAWalker2_validate -0.172656356414452 4.44580029427513e-21 NA NA NA hsa-miR-183-5p TXNIP 2.08412478942637 1.08931431254487e-10 -1.27660062513074 4.10601839671154e-10 miRNAWalker2_validate -0.424922995530094 8.07678262414498e-37 NA NA NA hsa-miR-18a-5p UBC 2.04701000113742 1.30593450319289e-12 -0.547103795643103 5.02187280305657e-11 miRNAWalker2_validate -0.108026549544118 1.35317425865265e-10 NA NA NA hsa-miR-200b-3p ULK2 2.06025765178663 3.07718049337497e-10 -0.507777916304274 0.00720267539139616 TargetScan -0.231597745104915 1.7212853574088e-12 NA NA NA hsa-miR-200b-3p USP27X 2.06025765178663 3.07718049337497e-10 0.105236875735044 0.40514235370682 TargetScan -0.109132570916822 9.90531261349787e-07 NA NA NA hsa-miR-21-5p UTRN 2.21515015440792 6.0468197386674e-49 -0.341307034762131 0.0355686209546459 miRNAWalker2_validate -0.182640141724198 0.000895317871009116 NA NA NA hsa-miR-200b-3p VAT1L 2.06025765178663 3.07718049337497e-10 -1.2975564288276 7.71112308641131e-06 TargetScan -0.276508933330425 1.11047093921134e-07 NA NA NA hsa-miR-200a-3p VCAM1 2.02394729844446 2.21240501925011e-08 0.619629702942566 0.00353301931056086 miRNAWalker2_validate -0.117289609012904 0.000778152184684981 NA NA NA hsa-miR-133a-3p VEGFA -2.63342156915404 5.72661351480585e-12 0.628775505110226 0.000620913144913639 miRNAWalker2_validate -0.17467432535846 2.12949891237168e-10 NA NA NA hsa-miR-192-5p VGLL3 2.0867723747501 3.57854249239079e-08 0.314863164469675 0.263033597488497 miRNAWalker2_validate -0.290539198846591 7.9478822269219e-12 NA NA NA hsa-miR-200b-3p VLDLR 2.06025765178663 3.07718049337497e-10 -1.51502351723723 4.10702622044204e-07 TargetScan -0.43727672776589 3.73272026772736e-17 NA NA NA hsa-miR-200b-3p WASF1 2.06025765178663 3.07718049337497e-10 -0.152219015266867 0.560115892861614 TargetScan -0.435700616723815 4.20078123559881e-24 NA NA NA hsa-miR-200a-3p WASF3 2.02394729844446 2.21240501925011e-08 -1.11115772222304 0.000353153562928233 miRNAWalker2_validate;miRTarBase -0.376128282411932 1.937796562519e-14 NA NA NA hsa-miR-200b-3p WASF3 2.06025765178663 3.07718049337497e-10 -1.11115772222304 0.000353153562928233 miRNAWalker2_validate;miRTarBase;TargetScan -0.412650541045337 2.0149023151053e-14 NA NA NA hsa-miR-200b-3p WBP1L 2.06025765178663 3.07718049337497e-10 -0.377971404363762 8.29956294454323e-05 TargetScan -0.147429960768648 1.03657509289249e-19 NA NA NA hsa-miR-200b-3p WDFY3 2.06025765178663 3.07718049337497e-10 -0.20090024293122 0.0966993995672952 TargetScan -0.121485683961825 9.89561489958834e-09 NA NA NA hsa-miR-192-5p WDR47 2.0867723747501 3.57854249239079e-08 -0.378252468795409 0.000312581763040392 miRNAWalker2_validate -0.164915602787692 1.48765020170802e-28 NA NA NA hsa-miR-21-5p WDR7 2.21515015440792 6.0468197386674e-49 -0.297418546307748 0.00599423951804285 miRNAWalker2_validate -0.151752314117167 3.08140035512846e-05 NA NA NA hsa-miR-21-5p WFS1 2.21515015440792 6.0468197386674e-49 -0.492565756614294 0.00737087241652513 miRNAWalker2_validate -0.381197160012256 3.64724592788954e-10 NA NA NA hsa-miR-200b-3p WIPF1 2.06025765178663 3.07718049337497e-10 -0.0481229578257576 0.794464532128527 TargetScan -0.215326976518172 1.71865385162294e-11 NA NA NA hsa-miR-200b-3p WIPF3 2.06025765178663 3.07718049337497e-10 -1.35945672119358 0.000118862043671656 TargetScan -0.306665675655209 1.33529389426248e-06 NA NA NA hsa-miR-192-5p WNK1 2.0867723747501 3.57854249239079e-08 -0.0928178691135297 0.446606934438845 miRNAWalker2_validate;miRTarBase -0.141712469582297 4.68841547031315e-15 NA NA NA hsa-miR-200b-3p WNK3 2.06025765178663 3.07718049337497e-10 -0.566384750663729 0.0267756841098298 TargetScan -0.399865177513822 1.13836539002506e-20 NA NA NA hsa-miR-21-5p WNK3 2.21515015440792 6.0468197386674e-49 -0.566384750663729 0.0267756841098298 miRNAWalker2_validate -0.371609508962061 1.60935349877209e-05 NA NA NA hsa-miR-192-5p WWC2 2.0867723747501 3.57854249239079e-08 -0.178838877224607 0.274326089669387 miRNAWalker2_validate -0.14740778103028 3.36627055735297e-09 NA NA NA hsa-miR-192-5p WWC3 2.0867723747501 3.57854249239079e-08 -0.16305955042508 0.336949478457286 miRNAWalker2_validate -0.242010157336886 3.85716005272087e-23 NA NA NA hsa-miR-200b-3p WWC3 2.06025765178663 3.07718049337497e-10 -0.16305955042508 0.336949478457286 TargetScan -0.292751821407836 4.52711450760642e-26 NA NA NA hsa-miR-18a-5p WWTR1 2.04701000113742 1.30593450319289e-12 -0.344530111808307 0.119970728404188 miRNAWalker2_validate -0.406755001122672 6.95966845412224e-23 NA NA NA hsa-miR-200b-3p XKR4 2.06025765178663 3.07718049337497e-10 -3.2550766725692 2.66020302369966e-10 TargetScan -0.918962368097352 8.61420278965142e-26 NA NA NA hsa-miR-200b-3p XKR6 2.06025765178663 3.07718049337497e-10 -0.834048035532205 0.00301699280624388 TargetScan -0.156073840954401 0.0021502227989225 NA NA NA hsa-miR-192-5p XPA 2.0867723747501 3.57854249239079e-08 -0.571355339471061 6.41096410445808e-08 miRNAWalker2_validate -0.104898666798798 1.5226334855311e-10 NA NA NA hsa-miR-200a-3p YAP1 2.02394729844446 2.21240501925011e-08 0.22036194497483 0.118363238667109 miRNAWalker2_validate -0.117592578563962 2.13977844197491e-07 23340296 MicroRNA 200a promotes anoikis resistance and metastasis by targeting YAP1 in human breast cancer; We identified Yes-associated protein 1 YAP1 as a novel target of miR-200a; Our data showed that targeting of YAP1 by miR-200a resulted in decreased expression of proapoptotic proteins which leads to anoikis resistance; Remarkably we found that YAP1 expression was inversely correlated with miR-200a expression in breast cancer clinical specimens and miR-200a expression was associated with distant metastasis in patients with breast cancer metastasis;drug resistance breast cancer hsa-miR-21-5p ZADH2 2.21515015440792 6.0468197386674e-49 -0.352837585404997 0.000188320740050818 miRNAWalker2_validate -0.143578260685888 6.48558360273449e-06 NA NA NA hsa-miR-192-5p ZBTB10 2.0867723747501 3.57854249239079e-08 0.11491226178643 0.650172271983559 miRNAWalker2_validate -0.203674259504496 1.65430198616637e-07 NA NA NA hsa-miR-200b-3p ZBTB16 2.06025765178663 3.07718049337497e-10 -2.91433902145166 3.46425121470516e-11 TargetScan -0.86148969433283 4.92164837141701e-32 NA NA NA hsa-miR-200b-3p ZBTB20 2.06025765178663 3.07718049337497e-10 -0.121915327498964 0.575686805536531 TargetScan -0.283541886018591 3.1220339678085e-14 NA NA NA hsa-miR-200b-3p ZBTB38 2.06025765178663 3.07718049337497e-10 -0.113347911967603 0.309588302506711 TargetScan -0.110520294452932 1.47620611057896e-08 NA NA NA hsa-miR-21-5p ZBTB47 2.21515015440792 6.0468197386674e-49 -0.487946101076992 0.00504998853219209 miRNAWalker2_validate -0.370960562758701 1.08270540506839e-10 NA NA NA hsa-miR-192-5p ZC3H12C 2.0867723747501 3.57854249239079e-08 -0.811123594545078 0.000172087654901931 miRNAWalker2_validate -0.105730396043265 0.0019596486242818 NA NA NA hsa-miR-200b-3p ZC3H12C 2.06025765178663 3.07718049337497e-10 -0.811123594545078 0.000172087654901931 TargetScan -0.194043905068367 5.12703872075676e-07 NA NA NA hsa-miR-200b-3p ZC3H6 2.06025765178663 3.07718049337497e-10 -0.421092141864099 0.00728833157985793 TargetScan -0.192624167520686 1.50696114427257e-12 NA NA NA hsa-miR-200b-3p ZCCHC24 2.06025765178663 3.07718049337497e-10 -0.738590343521926 0.00346932529454311 TargetScan -0.507821192062702 2.61547608277397e-37 NA NA NA hsa-miR-200b-3p ZDHHC17 2.06025765178663 3.07718049337497e-10 0.149454217669535 0.211398255202947 TargetScan -0.123966915831086 2.91087044370823e-09 NA NA NA hsa-miR-200a-3p ZEB1 2.02394729844446 2.21240501925011e-08 -0.474334019913817 0.0476440408394034 miRNAWalker2_validate;miRTarBase -0.451673396814164 6.48579234036771e-41 27402864;27402080;23743934;24815002;24424572;20473948;19854497;26887977;24390222;26242262;19544444;23626803;24037528 This circuit comprises the microRNA 200 miR-200 family ZEB1 and GRHL2;Conversely ectopic expression of miR-200 inhibited this sprouting by indirectly reducing the protein levels of PDGFRβ through the direct suppression of ZEB1;This finding was accompanied by a sharp downregulation in the expression of the miR-200 family of microRNAs well-known targets of ZEB1;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;ZEB1 is also known to repress miR-200c-141 transcription in a negative feedback loop but otherwise little is known about the transcriptional regulation of the miR-200 family and miR-205;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;GRHL2 miR 200 ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification;Inhibition of ZEB1 by miR 200 characterizes Helicobacter pylori positive gastric diffuse large B cell lymphoma with a less aggressive behavior; Through genome-wide expression profiles of both miRNAs and mRNAs in nine H pylori-positive and nine H pylori-negative gastric diffuse large B-cell lymphomas we identified inhibition of ZEB1 zinc-finger E-box-binding homeobox 1 by miR-200 in H pylori-positive gastric diffuse large B-cell lymphomas; In 30 H pylori-positive and 30 H pylori-negative gastric diffuse large B-cell lymphomas we confirmed that H pylori-positive gastric diffuse large B-cell lymphomas had higher levels of miR-200 by qRT-PCR and lower levels of ZEB1 and higher levels of BCL6 using immunohistochemistry;MiR 200 suppresses metastases of colorectal cancer through ZEB1; Here we showed that the levels of miR-200 were significantly decreased and the levels of ZEB1 were significantly increased in the CRC specimens from patients compared to the paired non-tumor tissue; Moreover the levels of miR-200 and ZEB1 are inversely correlated; Bioinformatics analyses showed that miR-200 targeted the 3'-UTR of ZEB1 mRNA to inhibit its translation which was confirmed by luciferase reporter assay;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2;Concomitant with miR-200 decrease there was an increase in ZEB1 mRNA expression;MiR 200 can repress breast cancer metastasis through ZEB1 independent but moesin dependent pathways ;;;;tumorigenesis;;;;;metastasis;;;metastasis sarcoma;breast cancer;endometrial cancer;sarcoma;head and neck cancer;bladder cancer;ovarian cancer;ovarian cancer;B cell lymphoma;colorectal cancer;prostate cancer;breast cancer;breast cancer hsa-miR-200b-3p ZEB1 2.06025765178663 3.07718049337497e-10 -0.474334019913817 0.0476440408394034 miRNAWalker2_validate;miRTarBase;TargetScan -0.486888008854174 6.10126603219397e-39 24352645;27402864;27402080;23743934;24815002;24424572;20473948;26011542;19854497;26887977;24390222;27307751;26242262;19544444;23626803;26783187;24037528 Functional experiments demonstrated that miR-200b suppressed ZEB1 augmented E-cadherin inhibited cell migration and suppressed tumor growth in a mouse model;This circuit comprises the microRNA 200 miR-200 family ZEB1 and GRHL2;Conversely ectopic expression of miR-200 inhibited this sprouting by indirectly reducing the protein levels of PDGFRβ through the direct suppression of ZEB1;This finding was accompanied by a sharp downregulation in the expression of the miR-200 family of microRNAs well-known targets of ZEB1;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;ZEB1 is also known to repress miR-200c-141 transcription in a negative feedback loop but otherwise little is known about the transcriptional regulation of the miR-200 family and miR-205;Here we show that 53BP1 negatively regulated EMT by modulating ZEB1 through targeting microRNA miR-200b and miR-429; In 18 clinical tissue samples expression of 53BP1 was positively correlated with miR-200b and mir-429 and negatively correlated with ZEB1;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;GRHL2 miR 200 ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification;Inhibition of ZEB1 by miR 200 characterizes Helicobacter pylori positive gastric diffuse large B cell lymphoma with a less aggressive behavior; Through genome-wide expression profiles of both miRNAs and mRNAs in nine H pylori-positive and nine H pylori-negative gastric diffuse large B-cell lymphomas we identified inhibition of ZEB1 zinc-finger E-box-binding homeobox 1 by miR-200 in H pylori-positive gastric diffuse large B-cell lymphomas; In 30 H pylori-positive and 30 H pylori-negative gastric diffuse large B-cell lymphomas we confirmed that H pylori-positive gastric diffuse large B-cell lymphomas had higher levels of miR-200 by qRT-PCR and lower levels of ZEB1 and higher levels of BCL6 using immunohistochemistry;MicroRNA 200b acts as a tumor suppressor in osteosarcoma via targeting ZEB1; Moreover ZEB1 was identified as a target gene of miR-200b and its expression levels were negatively mediated by miR-200b in osteosarcoma cells; Based on these findings our study suggests that miR-200b inhibits the proliferation migration and invasion of osteosarcoma cells probably via the inhibition of ZEB1 expression;MiR 200 suppresses metastases of colorectal cancer through ZEB1; Here we showed that the levels of miR-200 were significantly decreased and the levels of ZEB1 were significantly increased in the CRC specimens from patients compared to the paired non-tumor tissue; Moreover the levels of miR-200 and ZEB1 are inversely correlated; Bioinformatics analyses showed that miR-200 targeted the 3'-UTR of ZEB1 mRNA to inhibit its translation which was confirmed by luciferase reporter assay;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2;Concomitant with miR-200 decrease there was an increase in ZEB1 mRNA expression; Concomitant with the increase in miR-200b and miR-200c ZEB1 expression was decreased and cells appeared more epithelial in morphology and were sensitized to TAM and fulvestrant inhibition;Nintedanib was able to reverse TGF-β1-induced EMT and resistance to gefitinib caused by miR-200b and miR-141 upregulation and ZEB1 downregulation;MiR 200 can repress breast cancer metastasis through ZEB1 independent but moesin dependent pathways cell migration;;;;;tumorigenesis;;;;;;;metastasis;;;drug resistance;metastasis gastric cancer;sarcoma;breast cancer;endometrial cancer;sarcoma;head and neck cancer;bladder cancer;breast cancer;ovarian cancer;ovarian cancer;B cell lymphoma;sarcoma;colorectal cancer;prostate cancer;breast cancer;lung squamous cell cancer;breast cancer hsa-miR-200a-3p ZEB2 2.02394729844446 2.21240501925011e-08 -0.365164555864173 0.0476046539874367 miRNAWalker2_validate;miRTarBase -0.258941895617459 3.90063580596047e-20 24518294;19787069;24815002;24424572;19854497;21529905;19544444;25797260 This switch involved activation of the transforming growth factor-β TGFβ signaling network that led to a shift in the balance between miR-200 microRNAs and the transcription factor zinc finger E-box-binding homeobox 2 ZEB2 resulting in suppressed transcription of the gene encoding E-cadherin;These findings are surprising since the miR-200 family was previously shown to promote epithelial characteristics by inhibiting the transcriptional repressor Zeb2 and thereby enhancing E-cadherin expression; Expression of miR-200 which promotes a mesenchymal to epithelial cell transition MET by inhibiting Zeb2 expression unexpectedly enhances macroscopic metastases in mouse breast cancer cell lines;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;MicroRNA 200a inhibits CD133/1+ ovarian cancer stem cells migration and invasion by targeting E cadherin repressor ZEB2; The 3'-UTR of ZEB2 mRNA a transcriptional repressor of E-cadherin was found to be regulated directly by miR-200a; Our results suggest that loss of expression of miR-200a may play a critical role in the repression of E-cadherin by ZEB2 thereby enhancing migration and invasion in CD133/1+ cells;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2;MicroRNA 200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2; Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2; The associations between miR-200a SP cells and ZEB2 were validated in HCC; These findings reveal that miR-200a suppresses metastasis of SP cells by downregulating ZEB2 ;metastasis;;tumorigenesis;;;;metastasis breast cancer;breast cancer;sarcoma;head and neck cancer;ovarian cancer;ovarian cancer;prostate cancer;liver cancer hsa-miR-200b-3p ZEB2 2.06025765178663 3.07718049337497e-10 -0.365164555864173 0.0476046539874367 miRNAWalker2_validate;miRTarBase -0.270934795672967 3.64130357556405e-18 22311119;24518294;19787069;24815002;24424572;24769353;19854497;19544444 MicroRNA 200b regulates cell proliferation invasion and migration by directly targeting ZEB2 in gastric carcinoma; There was a strong correlation between the levels of miR-200b and ZEB2 and E-cadherin mRNAs in gastric cancer patients; miR-200b regulates ZEB2 expression and thus controls metastasis in gastric cancer;This switch involved activation of the transforming growth factor-β TGFβ signaling network that led to a shift in the balance between miR-200 microRNAs and the transcription factor zinc finger E-box-binding homeobox 2 ZEB2 resulting in suppressed transcription of the gene encoding E-cadherin;These findings are surprising since the miR-200 family was previously shown to promote epithelial characteristics by inhibiting the transcriptional repressor Zeb2 and thereby enhancing E-cadherin expression; Expression of miR-200 which promotes a mesenchymal to epithelial cell transition MET by inhibiting Zeb2 expression unexpectedly enhances macroscopic metastases in mouse breast cancer cell lines;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;Zinc finger E box binding homeobox 2 ZEB2 regulated by miR 200b contributes to multi drug resistance of small cell lung cancer; In silico analysis demonstrated that there are complementary binding sites between miR-200b and ZEB2 3'-UTR and identified miR-200b as a potential regulator of ZEB2; Overexpression of miR-200b led to the downregulation of ZEB2 at protein level; Luciferase reporter gene assay showed that 3'UTR ZEB2 activity was regulated by miR-200b; Our results suggest that ZEB2 modulates drug resistance and is regulated by miR-200b;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2 metastasis;;metastasis;;tumorigenesis;drug resistance;; gastric cancer;breast cancer;breast cancer;sarcoma;head and neck cancer;lung squamous cell cancer;ovarian cancer;prostate cancer hsa-miR-18a-5p ZFAND5 2.04701000113742 1.30593450319289e-12 -0.409420327651102 4.50112739233377e-05 miRNAWalker2_validate -0.139892207456502 1.12301628705601e-12 NA NA NA hsa-miR-200b-3p ZFAND5 2.06025765178663 3.07718049337497e-10 -0.409420327651102 4.50112739233377e-05 TargetScan -0.147293298653332 7.58162422760474e-18 NA NA NA hsa-miR-183-5p ZFHX4 2.08412478942637 1.08931431254487e-10 0.0184972294773713 0.955736809911936 miRNAWalker2_validate -0.503861543295932 6.56226597412138e-19 NA NA NA hsa-miR-200b-3p ZFHX4 2.06025765178663 3.07718049337497e-10 0.0184972294773713 0.955736809911936 TargetScan -0.48709569890708 4.86258543242825e-18 NA NA NA hsa-miR-192-5p ZFP1 2.0867723747501 3.57854249239079e-08 0.170647763782241 0.0848812036527689 miRNAWalker2_validate -0.137080260254138 8.12689551191585e-22 NA NA NA hsa-miR-192-5p ZFP30 2.0867723747501 3.57854249239079e-08 -0.019514163754248 0.929402972344817 miRNAWalker2_validate -0.14062593692418 3.80093627917942e-05 NA NA NA hsa-miR-935 ZFP37 2.321600496365 1.00042201726114e-07 -0.21724276710313 0.296313629252117 miRNAWalker2_validate -0.121590551798104 4.19249560787618e-05 NA NA NA hsa-miR-200a-3p ZFPM2 2.02394729844446 2.21240501925011e-08 -0.936440499022908 0.00110696573568431 miRNAWalker2_validate;miRTarBase -0.524500925681918 6.79251172542736e-37 26314828 It was reported that miR-200 can activate PI3K/AKT by targeting FOG2 friend of GATA 2 which directly binds to the p85α regulatory subunit of PI3K lung cancer hsa-miR-200b-3p ZFPM2 2.06025765178663 3.07718049337497e-10 -0.936440499022908 0.00110696573568431 miRNAWalker2_validate;miRTarBase;TargetScan -0.563191699295219 7.81638009550403e-35 26314828 It was reported that miR-200 can activate PI3K/AKT by targeting FOG2 friend of GATA 2 which directly binds to the p85α regulatory subunit of PI3K lung cancer hsa-miR-192-5p ZMAT3 2.0867723747501 3.57854249239079e-08 0.27771952020038 0.0499447683089053 miRNAWalker2_validate -0.107130302776379 9.38039268345691e-07 NA NA NA hsa-miR-192-5p ZMYND11 2.0867723747501 3.57854249239079e-08 -0.200269385015078 0.0544853551345854 miRNAWalker2_validate -0.12223719372149 2.50274850314908e-15 NA NA NA hsa-miR-192-5p ZNF248 2.0867723747501 3.57854249239079e-08 -0.0574258532196534 0.651465511774278 miRNAWalker2_validate -0.165013462263238 5.88020534617679e-19 NA NA NA hsa-miR-200b-3p ZNF362 2.06025765178663 3.07718049337497e-10 -0.231025493088191 0.0584071488986172 TargetScan -0.200793924424658 2.1400571543582e-23 NA NA NA hsa-miR-200b-3p ZNF365 2.06025765178663 3.07718049337497e-10 0.197703399755446 0.570502929787385 TargetScan -0.378679051297217 4.88003490795333e-10 NA NA NA hsa-miR-192-5p ZNF385B 2.0867723747501 3.57854249239079e-08 -2.04206623220296 7.71685969426973e-08 miRNAWalker2_validate -0.377806353523122 1.80778449563247e-10 NA NA NA hsa-miR-200b-3p ZNF423 2.06025765178663 3.07718049337497e-10 -0.207391944090895 0.423116370581353 TargetScan -0.441004141441394 2.76827175434507e-25 NA NA NA hsa-miR-192-5p ZNF449 2.0867723747501 3.57854249239079e-08 0.27714056761396 0.0126076680026678 miRNAWalker2_validate -0.112718962180074 1.9531410783621e-11 NA NA NA hsa-miR-200b-3p ZNF516 2.06025765178663 3.07718049337497e-10 -0.530173332560438 0.00170477542591741 TargetScan -0.22395458531804 1.61525877688981e-14 NA NA NA hsa-miR-192-5p ZNF519 2.0867723747501 3.57854249239079e-08 0.233250382314415 0.142886283627222 miRNAWalker2_validate -0.128385798936462 1.50770128692082e-07 NA NA NA hsa-miR-200b-3p ZNF532 2.06025765178663 3.07718049337497e-10 0.410685792071696 0.016269152211887 TargetScan -0.242068622468561 9.30894745115562e-17 NA NA NA hsa-miR-192-5p ZNF641 2.0867723747501 3.57854249239079e-08 -0.017286068034279 0.852780011752403 miRNAWalker2_validate -0.106043730781173 1.46076571738426e-14 NA NA NA hsa-miR-200b-3p ZNF662 2.06025765178663 3.07718049337497e-10 -1.88043061006014 2.81229008426511e-10 TargetScan -0.464721724566856 3.94538746603031e-19 NA NA NA hsa-miR-21-5p ZNF667 2.21515015440792 6.0468197386674e-49 -0.765209789722188 0.00575237963736784 miRNAWalker2_validate -0.44394090488413 1.92969073762543e-06 NA NA NA hsa-miR-192-5p ZNF704 2.0867723747501 3.57854249239079e-08 -0.446994599853389 0.116513806383905 miRNAWalker2_validate -0.159073483939168 0.000344526735688431 NA NA NA hsa-miR-192-5p ZNF85 2.0867723747501 3.57854249239079e-08 -0.290701683812118 0.152906418227546 miRNAWalker2_validate -0.109360241200319 0.000567121526582895 NA NA NA hsa-miR-21-5p ZRANB1 2.21515015440792 6.0468197386674e-49 -0.162987253940597 0.104519813914062 miRNAWalker2_validate -0.147657764003379 1.06163251655857e-05 NA NA NA hsa-miR-18a-5p ZSWIM6 2.04701000113742 1.30593450319289e-12 -0.433553897728624 1.52139610356126e-05 miRNAWalker2_validate -0.108153680512356 7.16900181044978e-08 NA NA NA hsa-miR-200b-3p ZYG11B 2.06025765178663 3.07718049337497e-10 -0.120538055007567 0.278257919741293 TargetScan -0.154191951640231 2.6373221635263e-16 NA NA NA hsa-miR-21-5p ZYG11B 2.21515015440792 6.0468197386674e-49 -0.120538055007567 0.278257919741293 miRNAWalker2_validate -0.11454476109684 0.00223807809844976 NA NA NA