miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-335-5p APAF1 0.348427694169649 0.0962171361436908 -0.0044985981329102 0.970113017391907 miRNAWalker2_validate -0.12931882528084 1.48612749421584e-06 NA NA NA hsa-miR-125a-3p ATM 0.766248892008454 5.97048811423325e-05 -0.945652583949022 1.3895005434567e-12 miRanda -0.11181303344535 0.000941443595940847 NA NA NA hsa-miR-186-5p ATM 1.01160002003511 1.0995153919986e-13 -0.945652583949022 1.3895005434567e-12 mirMAP -0.127748904919455 0.00631628674251774 NA NA NA hsa-miR-18a-5p ATM 3.22282140470889 1.9203457351048e-39 -0.945652583949022 1.3895005434567e-12 miRNAWalker2_validate;miRTarBase;MirTarget -0.122437766847937 2.89708575104783e-07 23437304;25963391;23857602;23229340 MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression colorectal cancer;cervical and endocervical cancer;breast cancer;colon cancer hsa-miR-324-5p ATM 2.14595874800649 3.03958987970622e-28 -0.945652583949022 1.3895005434567e-12 miRanda -0.157121054541443 3.98170646788431e-07 NA NA NA hsa-miR-339-5p ATM 1.79341790518551 3.04346641619717e-17 -0.945652583949022 1.3895005434567e-12 miRanda -0.157235214415106 6.41432567147416e-08 NA NA NA hsa-miR-421 ATM 2.10188043858202 8.21949037851434e-21 -0.945652583949022 1.3895005434567e-12 miRNAWalker2_validate;miRTarBase;MirTarget;miRanda -0.121126884863035 1.08710659687324e-05 NA NA NA hsa-miR-590-3p ATM 1.73212597984247 2.26504285146779e-20 -0.945652583949022 1.3895005434567e-12 miRanda;mirMAP -0.116304658195287 0.000454405904700417 NA NA NA hsa-miR-590-5p ATM 1.45684002317639 1.74263571329047e-15 -0.945652583949022 1.3895005434567e-12 mirMAP -0.131314156014943 0.000125752230520263 NA NA NA hsa-miR-766-3p ATM 2.69859152586189 7.66286160573257e-37 -0.945652583949022 1.3895005434567e-12 MirTarget -0.147296429325492 1.00615553207294e-07 NA NA NA hsa-miR-92a-3p ATM 1.69110879028255 8.04580501722806e-26 -0.945652583949022 1.3895005434567e-12 miRNAWalker2_validate -0.117581759290502 0.00206078677184739 NA NA NA hsa-miR-939-5p ATM 2.14910982072251 2.05036683100157e-20 -0.945652583949022 1.3895005434567e-12 MirTarget -0.1249618135545 1.03494153223572e-05 NA NA NA hsa-miR-101-3p BBC3 -1.48434639686706 2.22407930927359e-19 0.450943865698324 0.00976889921715259 miRNATAP -0.150879262483712 0.0017989267091693 NA NA NA hsa-miR-125a-5p BBC3 -0.347216996653838 0.0211699797450826 0.450943865698324 0.00976889921715259 miRNATAP -0.200396606488568 0.000285712905963502 NA NA NA hsa-miR-296-5p BBC3 -0.208779469897537 0.549226448418253 0.450943865698324 0.00976889921715259 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.101688866253766 2.4473782794703e-05 NA NA NA hsa-let-7c-5p CASP3 -1.7227919059598 9.51049211594794e-12 0.884665192818714 9.10079583585507e-18 MirTarget -0.112614277640154 4.8020157950459e-09 NA NA NA hsa-miR-101-3p CASP3 -1.48434639686706 2.22407930927359e-19 0.884665192818714 9.10079583585507e-18 MirTarget -0.198151616475033 7.27070415878007e-12 NA NA NA hsa-miR-139-5p CASP3 -2.41416537859302 6.88706484174732e-28 0.884665192818714 9.10079583585507e-18 miRanda -0.128159697416884 1.46578793258762e-09 NA NA NA hsa-miR-140-5p CASP3 -0.887888681168421 5.76545563709515e-08 0.884665192818714 9.10079583585507e-18 miRanda -0.129919659241298 2.03218620238873e-05 NA NA NA hsa-miR-195-3p CASP3 -1.78063568244372 2.34306663657327e-11 0.884665192818714 9.10079583585507e-18 MirTarget -0.113241114270892 7.52017710294153e-10 NA NA NA hsa-miR-26b-5p CASP3 0.300594892480765 0.0431916558729258 0.884665192818714 9.10079583585507e-18 miRNAWalker2_validate -0.124559804021021 0.000273540002705722 NA NA NA hsa-miR-28-5p CASP3 -0.466180213899448 0.00012482099258297 0.884665192818714 9.10079583585507e-18 miRanda -0.157383603168507 0.000173227334205312 NA NA NA hsa-miR-30c-5p CASP3 0.724673207831053 1.32226261564805e-05 0.884665192818714 9.10079583585507e-18 miRNATAP -0.100909486947423 0.000860093675866737 NA NA NA hsa-miR-374b-5p CASP3 -0.162562360514506 0.226063855202319 0.884665192818714 9.10079583585507e-18 mirMAP -0.133111689821541 0.000476760620971864 NA NA NA hsa-miR-26b-5p CASP8 0.300594892480765 0.0431916558729258 0.630101598022788 1.00673762626595e-06 miRNAWalker2_validate -0.124685560602374 0.00285786906194624 NA NA NA hsa-miR-139-5p CCNB1 -2.41416537859302 6.88706484174732e-28 3.13841902704964 7.78600770833339e-59 miRanda -0.35791341392326 1.86868420121427e-16 NA NA NA hsa-miR-140-5p CCNB1 -0.887888681168421 5.76545563709515e-08 3.13841902704964 7.78600770833339e-59 miRanda -0.341488377570726 6.77111416614409e-08 NA NA NA hsa-let-7c-5p CCNB2 -1.7227919059598 9.51049211594794e-12 4.70342071100617 1.02459768916783e-82 miRNAWalker2_validate -0.276480181771414 2.5323986118936e-07 NA NA NA hsa-miR-23b-3p CCNB2 -0.940125237183254 5.29255710761003e-10 4.70342071100617 1.02459768916783e-82 miRNAWalker2_validate -0.505716041390737 2.31524800035482e-08 NA NA NA hsa-let-7i-5p CCND1 0.158027124606232 0.194776988625516 0.774100490156041 0.00198050874034212 miRNATAP -0.243017558150684 0.0146232464714826 NA NA NA hsa-miR-106b-5p CCND1 2.17788294729604 3.35797658884941e-37 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.152767699847694 0.0174235843107592 NA NA NA hsa-miR-15b-5p CCND1 2.49680902955696 1.79560488884714e-45 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.159818257398952 0.0087089805971255 NA NA NA hsa-miR-186-5p CCND1 1.01160002003511 1.0995153919986e-13 0.774100490156041 0.00198050874034212 mirMAP -0.268543325467188 0.00175385379189439 NA NA NA hsa-miR-193a-3p CCND1 0.422281760608533 0.0531653219715062 0.774100490156041 0.00198050874034212 MirTarget;PITA;miRanda -0.178263529379242 0.00108591999879024 NA NA NA hsa-miR-195-5p CCND1 -1.81969168340723 1.04765314267131e-11 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.11528712596772 0.00792611405618288 21350001;26631043;25823925 Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497 miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer;MiR 195 inhibits the proliferation of human cervical cancer cells by directly targeting cyclin D1; The present study was to evaluate the level of miR-195 and cyclin D1 in CC tissues and cells; We further investigated the molecular mechanisms of miR-195 and cyclin D1 in CC cell lines HeLa and SiHa; Furthermore the expression of miR-195 was inversely proportional to that of cyclin D1 mRNA or protein p = 0.013 p = 0.015 respectively; However the inhibitor of miR-195 promoted the expression of cyclin D1 and cell proliferation; In conclusion our data suggest that miR-195 may have the potential role in treatment of CC patients as well as miR-195 is a novel regulator of invasiveness and tumorigenicity in CC cells by targeting cyclin D1;MicroRNA profiling identifies MiR 195 suppresses osteosarcoma cell metastasis by targeting CCND1; Meanwhile CCND1 was identified as the target gene of miR-195 and further studied; More importantly using real-time PCR we evaluated the expression of miR-195 and CCND1 in osteosarcoma samples from 107 frozen biopsy tissues and 99 formalin- or paraformalin-fixed paraffin-embedded FFPE tissues; Results indicated lowly expressed miR-195 or highly CCND1 correlated with positive overall survival and their expression inversely related to each other; In summary our study suggests miR-195 functions as a tumor metastasis suppressor gene by down-regulating CCND1 and can be used as a potential target in the treatment of osteosarcoma ;;metastasis;poor survival breast cancer;cervical and endocervical cancer;sarcoma hsa-miR-19b-1-5p CCND1 0.959999569197417 4.46371760817159e-06 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;miRTarBase -0.137896613562186 0.0148092524205043 NA NA NA hsa-miR-20a-5p CCND1 1.51276418035651 8.71307033346922e-13 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.150559180615601 0.00609187871508627 NA NA NA hsa-miR-29c-3p CCND1 -1.23721966533715 2.76723950051374e-09 0.774100490156041 0.00198050874034212 mirMAP -0.297887238189869 8.65092275182463e-08 NA NA NA hsa-miR-338-3p CCND1 0.429940853175601 0.097699268368127 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;miRTarBase;miRanda -0.102826328434132 0.0251539919361595 NA NA NA hsa-miR-340-5p CCND1 1.35294088371205 8.25205106713644e-16 0.774100490156041 0.00198050874034212 mirMAP -0.189631226648046 0.00588969925324404 NA NA NA hsa-miR-374a-5p CCND1 -0.0832626833277432 0.509637142371946 0.774100490156041 0.00198050874034212 MirTarget -0.392651244911889 3.85840419314101e-05 27191497 microRNA 374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway; Furthermore luciferase reporter assays confirmed that miR-374a could directly reduce CCND1; We examined miR-374a levels by in situ hybridization and its correlation with CCND1 expression in CRC tumor tissues; High miR-374a expression with low level of CCND1 was protective factor in CRC; Together these findings indicate that miR-374a inactivates the PI3K/AKT axis by inhibiting CCND1 suppressing of colon cancer progression progression colon cancer hsa-miR-374b-5p CCND1 -0.162562360514506 0.226063855202319 0.774100490156041 0.00198050874034212 miRNAWalker2_validate;MirTarget -0.282238115659266 0.00167086640493017 NA NA NA hsa-miR-497-5p CCND1 -0.944286652785117 0.000311488624920066 0.774100490156041 0.00198050874034212 MirTarget;miRNATAP -0.11347798866463 0.0120999832110802 21350001 Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497 miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer breast cancer hsa-let-7a-3p CCND2 1.03029456575871 2.05932484675687e-08 -2.90637368197601 7.97867652738068e-25 mirMAP -0.239581067529379 0.00165944555482354 20418948 MicroRNA let 7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2 prostate cancer hsa-let-7e-5p CCND2 0.821771857232799 1.19334423679144e-10 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.379041562244543 0.000572917419499884 NA NA NA hsa-let-7f-1-3p CCND2 0.993720810761227 6.0407149976796e-08 -2.90637368197601 7.97867652738068e-25 mirMAP -0.237322955175854 0.00199587587185861 NA NA NA hsa-let-7g-5p CCND2 1.15803586058037 1.01768088555577e-12 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.344169378998088 5.28363064149805e-05 NA NA NA hsa-miR-106a-5p CCND2 2.93836356285531 1.22956096158575e-21 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.313743308662334 2.05139444242571e-13 NA NA NA hsa-miR-106b-5p CCND2 2.17788294729604 3.35797658884941e-37 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;miRTarBase;miRNATAP -0.666857943590157 9.08265679398644e-20 NA NA NA hsa-miR-10a-3p CCND2 2.65415484977961 4.06781213095544e-14 -2.90637368197601 7.97867652738068e-25 mirMAP -0.213456724219775 6.77002410328012e-08 NA NA NA hsa-miR-130b-5p CCND2 3.62170467703245 4.5670833909813e-51 -2.90637368197601 7.97867652738068e-25 mirMAP -0.384705597186037 3.45247419331457e-14 NA NA NA hsa-miR-141-3p CCND2 4.68123467996176 4.92783859837668e-50 -2.90637368197601 7.97867652738068e-25 MirTarget;TargetScan -0.367774092444505 5.01448255347277e-22 NA NA NA hsa-miR-151a-3p CCND2 0.944646013243785 4.69249786473818e-12 -2.90637368197601 7.97867652738068e-25 mirMAP -0.673402772305367 1.87631547505508e-11 NA NA NA hsa-miR-15a-5p CCND2 1.77791080875961 1.15367449842886e-31 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;miRTarBase;miRNATAP -0.624454857314169 2.2872795020307e-13 NA NA NA hsa-miR-15b-5p CCND2 2.49680902955696 1.79560488884714e-45 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.732291578179398 8.47926085446095e-27 NA NA NA hsa-miR-16-2-3p CCND2 2.33811104285535 7.35637935757058e-33 -2.90637368197601 7.97867652738068e-25 mirMAP -0.503613669385463 1.36053487006764e-14 NA NA NA hsa-miR-16-5p CCND2 1.88120757008208 1.6207082610779e-32 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;miRNATAP -0.598387483165678 1.92775144811544e-13 NA NA NA hsa-miR-17-5p CCND2 2.26819952852251 1.17080117367962e-29 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.518281285706455 5.94763144673765e-16 NA NA NA hsa-miR-181a-2-3p CCND2 1.64774852020118 8.81174897688593e-16 -2.90637368197601 7.97867652738068e-25 mirMAP -0.326012699547066 9.13706234118163e-07 NA NA NA hsa-miR-182-5p CCND2 5.17636717835373 1.04663621342014e-76 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;miRTarBase;miRNATAP -0.370617042021599 7.62910456371103e-20 NA NA NA hsa-miR-183-5p CCND2 6.01612233013103 1.47372794176386e-93 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.374086014133305 3.55147116947754e-24 NA NA NA hsa-miR-185-5p CCND2 1.98430335062845 1.43827631289268e-30 -2.90637368197601 7.97867652738068e-25 MirTarget;miRNATAP -0.571662942342317 1.98173786373456e-14 NA NA NA hsa-miR-186-5p CCND2 1.01160002003511 1.0995153919986e-13 -2.90637368197601 7.97867652738068e-25 mirMAP;miRNATAP -0.502985123613246 6.93914998225383e-07 NA NA NA hsa-miR-188-5p CCND2 1.83180846049089 3.69593490679723e-16 -2.90637368197601 7.97867652738068e-25 PITA;mirMAP -0.322873532447012 8.89540492036421e-08 NA NA NA hsa-miR-191-5p CCND2 2.2511568694408 2.00372706322068e-33 -2.90637368197601 7.97867652738068e-25 MirTarget -0.487502158027012 1.35184568144537e-12 NA NA NA hsa-miR-19a-3p CCND2 1.84830198357993 2.4710712042826e-20 -2.90637368197601 7.97867652738068e-25 MirTarget;miRNATAP -0.459148810183709 4.60812358943953e-12 NA NA NA hsa-miR-19b-3p CCND2 1.34004947278979 5.26920324973452e-16 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;MirTarget;miRNATAP -0.511589224588263 4.14932814576383e-10 NA NA NA hsa-miR-200a-3p CCND2 4.83505960801065 7.294437616154e-52 -2.90637368197601 7.97867652738068e-25 MirTarget -0.348983381438399 1.57989286386983e-20 NA NA NA hsa-miR-20a-5p CCND2 1.51276418035651 8.71307033346922e-13 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;miRTarBase;miRNATAP -0.419236880588012 6.06210145860637e-11 NA NA NA hsa-miR-20b-5p CCND2 3.35823156722934 1.69420622814985e-14 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.186348147303127 1.42773670498465e-09 NA NA NA hsa-miR-21-3p CCND2 2.45285942153002 1.98708014900155e-29 -2.90637368197601 7.97867652738068e-25 mirMAP -0.285737347792203 2.23714792602755e-06 NA NA NA hsa-miR-301a-3p CCND2 1.9416887079489 3.80655048022936e-21 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate -0.420988806888484 7.43152803674369e-11 NA NA NA hsa-miR-3065-3p CCND2 2.26276131398567 7.32764250522453e-13 -2.90637368197601 7.97867652738068e-25 MirTarget;miRNATAP -0.243208368884993 1.64309205118639e-08 NA NA NA hsa-miR-3065-5p CCND2 2.75496955380748 2.37091362562179e-15 -2.90637368197601 7.97867652738068e-25 mirMAP -0.228575985591394 2.17441983100348e-08 NA NA NA hsa-miR-30d-3p CCND2 0.755916398923689 3.64952022092868e-05 -2.90637368197601 7.97867652738068e-25 mirMAP -0.457394079234807 1.79359258470357e-09 NA NA NA hsa-miR-32-3p CCND2 2.40467177052961 5.81388257063569e-26 -2.90637368197601 7.97867652738068e-25 mirMAP -0.371884280518271 1.90283166643037e-09 NA NA NA hsa-miR-320a CCND2 0.502704491334741 0.00226252073794899 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate;mirMAP;miRNATAP -0.229909744909998 0.00781092664804684 NA NA NA hsa-miR-320b CCND2 1.09552613515995 1.0755423833529e-07 -2.90637368197601 7.97867652738068e-25 mirMAP;miRNATAP -0.308816018612761 4.69401568287548e-06 NA NA NA hsa-miR-320c CCND2 0.733362580458909 0.00057735467707277 -2.90637368197601 7.97867652738068e-25 mirMAP;miRNATAP -0.182233464403321 0.00731429232288534 NA NA NA hsa-miR-324-3p CCND2 2.34606663412817 2.38165582241626e-31 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate -0.57687571371449 4.40324487459554e-20 NA NA NA hsa-miR-331-5p CCND2 1.35722430593336 4.30006931857061e-16 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.420606003296683 2.56074439450733e-07 NA NA NA hsa-miR-33a-3p CCND2 1.39444251212239 7.26586547385458e-13 -2.90637368197601 7.97867652738068e-25 MirTarget -0.329871026439033 3.38635357606979e-06 NA NA NA hsa-miR-378a-3p CCND2 0.410763091457073 0.0978801515654437 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate -0.185551375767321 0.00114535077061192 NA NA NA hsa-miR-423-5p CCND2 0.875722574439598 4.995026399727e-09 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate -0.351632739043153 0.000170314534637264 NA NA NA hsa-miR-429 CCND2 5.03984117897747 4.44936205318976e-55 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.347355164269461 4.29890376916759e-21 NA NA NA hsa-miR-486-5p CCND2 0.630265651169473 0.0356088002128703 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.129319408756683 0.0061107119093723 NA NA NA hsa-miR-500a-5p CCND2 0.803568840489829 3.89317331148068e-05 -2.90637368197601 7.97867652738068e-25 mirMAP -0.237021283107101 0.00106011865518069 NA NA NA hsa-miR-501-5p CCND2 1.63365089104021 2.93689344466334e-15 -2.90637368197601 7.97867652738068e-25 PITA;mirMAP;miRNATAP -0.391911027120537 2.13648698521908e-09 NA NA NA hsa-miR-548v CCND2 1.69887322011149 1.0523433577007e-09 -2.90637368197601 7.97867652738068e-25 MirTarget -0.229547466478604 4.78218070433852e-06 NA NA NA hsa-miR-550a-5p CCND2 1.68852119077479 6.89894923025492e-17 -2.90637368197601 7.97867652738068e-25 MirTarget -0.466819104647831 1.90642347507647e-12 NA NA NA hsa-miR-577 CCND2 3.23403680126545 4.85573112916202e-14 -2.90637368197601 7.97867652738068e-25 PITA;mirMAP -0.150789621331072 3.16764407975412e-06 NA NA NA hsa-miR-589-3p CCND2 1.69712322956823 3.70306979263175e-12 -2.90637368197601 7.97867652738068e-25 mirMAP -0.299963877442968 1.58263259804119e-07 NA NA NA hsa-miR-590-3p CCND2 1.73212597984247 2.26504285146779e-20 -2.90637368197601 7.97867652738068e-25 miRanda;mirMAP -0.354766388840114 8.56085290699691e-07 NA NA NA hsa-miR-590-5p CCND2 1.45684002317639 1.74263571329047e-15 -2.90637368197601 7.97867652738068e-25 mirMAP -0.455566644209712 7.21054023862933e-10 NA NA NA hsa-miR-616-5p CCND2 1.97053352577988 6.50616168455898e-16 -2.90637368197601 7.97867652738068e-25 mirMAP -0.304230434157111 4.94935772330521e-08 NA NA NA hsa-miR-660-5p CCND2 0.20661370223084 0.267201322788119 -2.90637368197601 7.97867652738068e-25 mirMAP -0.197818759351872 0.00979078417631556 NA NA NA hsa-miR-7-1-3p CCND2 1.83538862227934 3.8339472676544e-18 -2.90637368197601 7.97867652738068e-25 mirMAP -0.349224846258081 4.30300182101647e-08 NA NA NA hsa-miR-877-5p CCND2 3.91787629016903 1.89259486237444e-39 -2.90637368197601 7.97867652738068e-25 miRNAWalker2_validate -0.357475468626144 2.20951038137177e-14 NA NA NA hsa-miR-9-3p CCND2 2.20853188955728 2.21538083391586e-07 -2.90637368197601 7.97867652738068e-25 MirTarget;mirMAP;miRNATAP -0.157580265255124 1.39277766224664e-06 NA NA NA hsa-miR-93-5p CCND2 2.57501728456111 3.73950852324834e-39 -2.90637368197601 7.97867652738068e-25 miRNATAP -0.65719306978185 4.56813339327463e-26 NA NA NA hsa-miR-96-5p CCND2 5.73185523568009 7.6326624678818e-88 -2.90637368197601 7.97867652738068e-25 TargetScan;miRNATAP -0.376449733310708 4.60956893816592e-23 NA NA NA hsa-miR-125b-5p CCNE1 -1.41889699079623 1.55084253952861e-08 3.878770582232 1.08532647092037e-40 miRNAWalker2_validate -0.254594388366061 1.62698208517514e-05 NA NA NA hsa-miR-195-5p CCNE1 -1.81969168340723 1.04765314267131e-11 3.878770582232 1.08532647092037e-40 miRNAWalker2_validate;MirTarget;miRNATAP -0.503995447401969 1.17829370872545e-21 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.631973911825762 2.12482912864596e-07 3.878770582232 1.08532647092037e-40 miRNAWalker2_validate;miRTarBase;miRNATAP -0.287862112843964 0.0212242069959781 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-424-5p CCNE1 -2.23160783176865 1.2307369299943e-15 3.878770582232 1.08532647092037e-40 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.358410566437467 2.71949751486714e-12 NA NA NA hsa-miR-497-5p CCNE1 -0.944286652785117 0.000311488624920066 3.878770582232 1.08532647092037e-40 MirTarget;miRNATAP -0.423520562277365 3.66065527064593e-14 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-126-3p CCNE2 -0.243150308745207 0.109583503635541 2.31927237338687 3.5804825336408e-18 miRNAWalker2_validate -0.236078493048986 0.00697503091668904 NA NA NA hsa-miR-140-5p CCNE2 -0.887888681168421 5.76545563709515e-08 2.31927237338687 3.5804825336408e-18 miRanda -0.410731277597876 1.98914718797002e-07 NA NA NA hsa-miR-26a-5p CCNE2 -0.631973911825762 2.12482912864596e-07 2.31927237338687 3.5804825336408e-18 miRNAWalker2_validate;miRTarBase;miRNATAP -0.620491421961091 6.43149529944205e-09 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-26b-5p CCNE2 0.300594892480765 0.0431916558729258 2.31927237338687 3.5804825336408e-18 miRNATAP -0.259177074398807 0.00372623001967479 NA NA NA hsa-miR-30a-5p CCNE2 0.204006364430255 0.420323839030139 2.31927237338687 3.5804825336408e-18 miRNATAP -0.17553192750384 0.000734098959294668 NA NA NA hsa-let-7e-5p CCNG1 0.821771857232799 1.19334423679144e-10 -0.675940428622058 7.91772042473665e-07 miRNAWalker2_validate -0.115630799709059 0.0235477084111507 NA NA NA hsa-miR-142-5p CCNG1 1.99051987846668 1.30874707506504e-14 -0.675940428622058 7.91772042473665e-07 mirMAP -0.107330106923716 1.10186125095206e-05 NA NA NA hsa-miR-21-5p CCNG1 1.51489362035879 4.99155235011048e-20 -0.675940428622058 7.91772042473665e-07 miRNAWalker2_validate -0.101890063123798 0.00756444257003557 NA NA NA hsa-miR-23a-3p CCNG1 1.0834199821542 3.34616494480896e-13 -0.675940428622058 7.91772042473665e-07 MirTarget;miRNATAP -0.212973190073614 6.47514348265785e-07 NA NA NA hsa-miR-24-3p CCNG1 0.861226137512247 2.90830475095671e-09 -0.675940428622058 7.91772042473665e-07 miRNAWalker2_validate -0.231569184907106 1.74009129394358e-07 NA NA NA hsa-miR-27a-3p CCNG1 1.20835958303386 1.23147423005989e-12 -0.675940428622058 7.91772042473665e-07 MirTarget;miRNATAP -0.210036844152323 1.66319312347609e-08 NA NA NA hsa-miR-335-3p CCNG1 1.32116009044055 1.0694366259168e-09 -0.675940428622058 7.91772042473665e-07 MirTarget;mirMAP -0.114905505166578 0.000103886793834707 NA NA NA hsa-miR-339-5p CCNG1 1.79341790518551 3.04346641619717e-17 -0.675940428622058 7.91772042473665e-07 miRanda -0.126997477406951 1.76466077520802e-05 NA NA NA hsa-miR-590-3p CCNG1 1.73212597984247 2.26504285146779e-20 -0.675940428622058 7.91772042473665e-07 miRanda -0.141615741264018 2.27761821192057e-05 NA NA NA hsa-miR-590-5p CCNG1 1.45684002317639 1.74263571329047e-15 -0.675940428622058 7.91772042473665e-07 miRanda -0.138700448640932 6.10341577965484e-05 NA NA NA hsa-miR-96-5p CCNG1 5.73185523568009 7.6326624678818e-88 -0.675940428622058 7.91772042473665e-07 MirTarget;TargetScan -0.105983061802625 6.09750218396257e-09 NA NA NA hsa-let-7a-3p CCNG2 1.03029456575871 2.05932484675687e-08 -0.244614020027427 0.0746242307970298 MirTarget;mirMAP -0.143563020875577 3.56340438629384e-05 NA NA NA hsa-let-7f-1-3p CCNG2 0.993720810761227 6.0407149976796e-08 -0.244614020027427 0.0746242307970298 MirTarget;mirMAP -0.130581288585358 0.000195830599707576 NA NA NA hsa-miR-142-5p CCNG2 1.99051987846668 1.30874707506504e-14 -0.244614020027427 0.0746242307970298 MirTarget;miRNATAP -0.141757699109127 3.32260593391446e-09 NA NA NA hsa-miR-16-2-3p CCNG2 2.33811104285535 7.35637935757058e-33 -0.244614020027427 0.0746242307970298 mirMAP -0.108457282933846 0.000417365081338729 NA NA NA hsa-miR-26a-2-3p CCNG2 0.0966122083896723 0.587572958861438 -0.244614020027427 0.0746242307970298 mirMAP -0.10562214240725 0.00396613805064432 NA NA NA hsa-miR-26a-5p CCNG2 -0.631973911825762 2.12482912864596e-07 -0.244614020027427 0.0746242307970298 mirMAP -0.146707573615938 0.00605359012745132 NA NA NA hsa-miR-26b-3p CCNG2 0.535170519177007 0.00102354513515568 -0.244614020027427 0.0746242307970298 MirTarget -0.132471937467975 0.000887954403349818 NA NA NA hsa-miR-26b-5p CCNG2 0.300594892480765 0.0431916558729258 -0.244614020027427 0.0746242307970298 mirMAP -0.204362694105687 2.91177501758955e-06 NA NA NA hsa-miR-331-5p CCNG2 1.35722430593336 4.30006931857061e-16 -0.244614020027427 0.0746242307970298 miRNATAP -0.153597289694475 4.34419957816026e-05 NA NA NA hsa-miR-335-5p CCNG2 0.348427694169649 0.0962171361436908 -0.244614020027427 0.0746242307970298 miRNAWalker2_validate;mirMAP -0.160017092121843 1.90053223071186e-07 NA NA NA hsa-miR-342-3p CCNG2 0.909988426908683 3.51879055202261e-09 -0.244614020027427 0.0746242307970298 miRanda -0.22902068731976 2.65852093527508e-08 NA NA NA hsa-miR-374b-5p CCNG2 -0.162562360514506 0.226063855202319 -0.244614020027427 0.0746242307970298 mirMAP -0.194470675275194 6.69636944713187e-05 NA NA NA hsa-miR-590-3p CCNG2 1.73212597984247 2.26504285146779e-20 -0.244614020027427 0.0746242307970298 miRanda;mirMAP -0.128838811621092 0.00010232332197756 NA NA NA hsa-miR-590-5p CCNG2 1.45684002317639 1.74263571329047e-15 -0.244614020027427 0.0746242307970298 mirMAP -0.147076089992545 1.72186944878187e-05 NA NA NA hsa-miR-127-3p CD82 -1.34817874284588 4.07880590250144e-07 0.668719987260467 0.0060301980223866 miRanda -0.223663311870521 1.34365035003627e-07 NA NA NA hsa-miR-140-5p CDK2 -0.887888681168421 5.76545563709515e-08 0.528154620901153 4.60203148465343e-05 miRanda -0.178534641722825 1.50051396536405e-06 NA NA NA hsa-miR-23b-3p CDK2 -0.940125237183254 5.29255710761003e-10 0.528154620901153 4.60203148465343e-05 miRNAWalker2_validate -0.124284609750097 0.00203924083201326 NA NA NA hsa-miR-26b-5p CDK2 0.300594892480765 0.0431916558729258 0.528154620901153 4.60203148465343e-05 miRNAWalker2_validate -0.134906430982117 0.00126418803440371 NA NA NA hsa-miR-195-5p CDK4 -1.81969168340723 1.04765314267131e-11 0.304519375063458 0.00478362156586663 miRNAWalker2_validate;miRTarBase -0.118629908598279 8.36678837560686e-11 NA NA NA hsa-let-7a-3p CDK6 1.03029456575871 2.05932484675687e-08 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.298439994418427 3.82070891926398e-06 NA NA NA hsa-let-7a-5p CDK6 0.0957603590789535 0.432888163037337 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase;TargetScan -0.57621762630461 6.88578022170073e-09 NA NA NA hsa-let-7b-3p CDK6 0.348643846906648 0.0705621714966111 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.26076123240432 3.13241710557565e-05 NA NA NA hsa-let-7b-5p CDK6 -0.0749477218001626 0.651851405417039 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase -0.366431301236386 4.66777599762871e-07 NA NA NA hsa-miR-106b-5p CDK6 2.17788294729604 3.35797658884941e-37 -0.989014950817131 9.56372271996119e-05 mirMAP -0.15352580002907 0.0187131042493937 NA NA NA hsa-miR-107 CDK6 1.80682322226628 2.47002735902976e-27 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.197468613640784 0.00392235993822929 19407485;22491216;21264532;19688090 Enforced expression of miR-107 in MiaPACA-2 and PANC-1 cells downregulated in vitro growth and this was associated with repression of the putative miR-107 target cyclin-dependent kinase 6 thereby providing a functional basis for the epigenetic inactivation of this miRNA in pancreatic cancer;Levels of known miR-107 targets protein kinase Cε PKCε cyclin-dependent kinase 6 CDK6 and hypoxia-inducible factor 1-β HIF1-β decreased following NP/pre-miR-107 treatment;We have identified miR-107 as a potential regulator of CDK6 expression; A bioinformatics search revealed a putative target site for miR-107 within the CDK6 3' untranslated region; Expression of miR-107 in gastric cancer cell lines was found inversely correlated with CDK6 expression; miR-107 could significantly suppress CDK6 3' UTR luciferase reporter activity and this effect was not detectable when the putative 3' UTR target site was mutated; Consistent with the results of the reporter assay ectopic expression of miR-107 reduced both mRNA and protein expression levels of CDK6 inhibited proliferation induced G1 cell cycle arrest and blocked invasion of the gastric cancer cells; Our results suggest that miR-107 may have a tumor suppressor function by directly targeting CDK6 to inhibit the proliferation and invasion activities of gastric cancer cells;Using miRNA-target prediction analyses and the array data we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6 ;;; pancreatic cancer;head and neck cancer;gastric cancer;lung squamous cell cancer hsa-miR-1254 CDK6 2.6936072043758 2.69365736193576e-22 -0.989014950817131 9.56372271996119e-05 PITA;miRNATAP -0.108417739878534 0.0177270172829199 NA NA NA hsa-miR-141-3p CDK6 4.68123467996176 4.92783859837668e-50 -0.989014950817131 9.56372271996119e-05 TargetScan;miRNATAP -0.20034360746943 2.43617228219254e-09 NA NA NA hsa-miR-148b-3p CDK6 1.80941322303736 4.89099431390024e-33 -0.989014950817131 9.56372271996119e-05 mirMAP -0.355969791002053 1.5372084785805e-06 NA NA NA hsa-miR-148b-5p CDK6 2.24566117508049 6.39491669468652e-28 -0.989014950817131 9.56372271996119e-05 mirMAP -0.229197409674557 3.3781419561209e-05 NA NA NA hsa-miR-15a-5p CDK6 1.77791080875961 1.15367449842886e-31 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.444168582591339 1.42892096802546e-09 NA NA NA hsa-miR-16-1-3p CDK6 2.08698516922856 1.42419622445015e-24 -0.989014950817131 9.56372271996119e-05 mirMAP -0.282016321134934 4.1332595569613e-07 NA NA NA hsa-miR-16-5p CDK6 1.88120757008208 1.6207082610779e-32 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase -0.421935840641667 1.78974719024504e-09 NA NA NA hsa-miR-182-5p CDK6 5.17636717835373 1.04663621342014e-76 -0.989014950817131 9.56372271996119e-05 mirMAP -0.186618126133005 1.83281335671308e-07 NA NA NA hsa-miR-185-5p CDK6 1.98430335062845 1.43827631289268e-30 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate -0.206784540780364 0.00159785534963276 19688090 Using miRNA-target prediction analyses and the array data we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6 lung squamous cell cancer hsa-miR-191-5p CDK6 2.2511568694408 2.00372706322068e-33 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase -0.32508674333528 4.32600782953417e-08 NA NA NA hsa-miR-193b-3p CDK6 1.19715471134498 1.60271373616177e-07 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate -0.174305015948811 0.000838861376722521 NA NA NA hsa-miR-195-5p CDK6 -1.81969168340723 1.04765314267131e-11 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase -0.101265593139456 0.0218612639907632 23333942 Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378 respectively gastric cancer hsa-miR-1976 CDK6 2.88535661397958 1.06588775996244e-47 -0.989014950817131 9.56372271996119e-05 mirMAP -0.293751566521881 7.94549952547701e-08 NA NA NA hsa-miR-200a-3p CDK6 4.83505960801065 7.294437616154e-52 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.189742237199017 9.19654881509274e-09 24009066 microRNA 200a is an independent prognostic factor of hepatocellular carcinoma and induces cell cycle arrest by targeting CDK6 liver cancer hsa-miR-200b-3p CDK6 4.56508753729609 3.8956399692993e-55 -0.989014950817131 9.56372271996119e-05 mirMAP -0.222567935435096 4.69243516033353e-10 NA NA NA hsa-miR-200c-3p CDK6 4.19740435353621 6.07734417092297e-49 -0.989014950817131 9.56372271996119e-05 mirMAP -0.208168804043929 2.26118944080953e-08 NA NA NA hsa-miR-30b-5p CDK6 0.359198073795339 0.0288588301689338 -0.989014950817131 9.56372271996119e-05 mirMAP -0.218011042458727 0.00322098361897355 NA NA NA hsa-miR-30d-3p CDK6 0.755916398923689 3.64952022092868e-05 -0.989014950817131 9.56372271996119e-05 mirMAP -0.193437065798113 0.00333325737637459 NA NA NA hsa-miR-30d-5p CDK6 0.577677794903206 0.000226725362226714 -0.989014950817131 9.56372271996119e-05 mirMAP -0.200470959230045 0.00962403716669674 NA NA NA hsa-miR-30e-3p CDK6 0.124986008379077 0.345651708234895 -0.989014950817131 9.56372271996119e-05 mirMAP -0.260905029027125 0.00485392713025478 NA NA NA hsa-miR-32-3p CDK6 2.40467177052961 5.81388257063569e-26 -0.989014950817131 9.56372271996119e-05 mirMAP -0.227641313562445 1.9992273124124e-05 NA NA NA hsa-miR-32-5p CDK6 1.61585199704519 4.93530744182272e-20 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.196595361742743 0.00287746756816587 NA NA NA hsa-miR-320a CDK6 0.502704491334741 0.00226252073794899 -0.989014950817131 9.56372271996119e-05 PITA;miRNATAP -0.255534851558063 0.000511961277629095 NA NA NA hsa-miR-320b CDK6 1.09552613515995 1.0755423833529e-07 -0.989014950817131 9.56372271996119e-05 PITA;miRNATAP -0.181350880210728 0.00174476086728566 NA NA NA hsa-miR-330-3p CDK6 1.76420587112262 1.73634190017122e-17 -0.989014950817131 9.56372271996119e-05 PITA;mirMAP -0.1217455964381 0.0311885387214421 NA NA NA hsa-miR-33a-5p CDK6 1.04549935225884 3.96713310508022e-05 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.1917228331574 4.61542751639555e-05 NA NA NA hsa-miR-33b-5p CDK6 2.2645934238776 2.84310918328736e-13 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.262155530769159 8.64618265016321e-12 NA NA NA hsa-miR-340-5p CDK6 1.35294088371205 8.25205106713644e-16 -0.989014950817131 9.56372271996119e-05 mirMAP -0.24699236859303 0.000400749773005368 NA NA NA hsa-miR-34a-5p CDK6 0.944430336151223 7.7422387648004e-06 -0.989014950817131 9.56372271996119e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.25139091658275 8.54896619351589e-06 21702042;26104764 Molecular analyses identified Cdk6 and sirtuin SIRT-1 as being targeted by miR-34a in MI-TCC cells however inhibition of Cdk6 and SIRT-1 was not as effective as pre-miR-34a in mediating chemosensitization;The expression of microRNA 34a is inversely correlated with c MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients; We found significant inverse correlations between miR-34a and c-MET R = -0.316 P = 0.028 and CDK6 expression R = -0.4582 P = 0.004 ; bladder cancer;lung cancer hsa-miR-3613-5p CDK6 2.36161077591908 1.58945209479103e-28 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.227382917410739 1.76121689278701e-05 NA NA NA hsa-miR-362-5p CDK6 0.449840584046766 0.0292542553380675 -0.989014950817131 9.56372271996119e-05 mirMAP -0.201630727928006 0.000587367308927917 NA NA NA hsa-miR-374a-3p CDK6 0.0941875095170328 0.532789406241938 -0.989014950817131 9.56372271996119e-05 mirMAP -0.209791622221342 0.00962417208251313 NA NA NA hsa-miR-378a-3p CDK6 0.410763091457073 0.0978801515654437 -0.989014950817131 9.56372271996119e-05 mirMAP -0.154683548660202 0.00149287954689365 23333942 Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378 respectively gastric cancer hsa-miR-421 CDK6 2.10188043858202 8.21949037851434e-21 -0.989014950817131 9.56372271996119e-05 mirMAP;miRNATAP -0.103289308611984 0.0463665678950571 NA NA NA hsa-miR-425-5p CDK6 3.37583546071413 8.59535002332206e-48 -0.989014950817131 9.56372271996119e-05 mirMAP -0.185010592591685 6.50207889634111e-05 NA NA NA hsa-miR-429 CDK6 5.03984117897747 4.44936205318976e-55 -0.989014950817131 9.56372271996119e-05 mirMAP;miRNATAP -0.192911788371378 2.60110012786824e-09 NA NA NA hsa-miR-491-3p CDK6 1.81866680232091 1.93335045253798e-15 -0.989014950817131 9.56372271996119e-05 PITA;miRNATAP -0.178762175466784 0.000587697927367447 NA NA NA hsa-miR-497-5p CDK6 -0.944286652785117 0.000311488624920066 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.139002852038347 0.00244899976309475 NA NA NA hsa-miR-500a-5p CDK6 0.803568840489829 3.89317331148068e-05 -0.989014950817131 9.56372271996119e-05 mirMAP -0.159868671821237 0.00982806206449146 NA NA NA hsa-miR-501-3p CDK6 2.18792856444299 6.3664407099549e-25 -0.989014950817131 9.56372271996119e-05 PITA;TargetScan;miRNATAP -0.211521079600504 8.2084974689421e-05 NA NA NA hsa-miR-502-3p CDK6 0.42804205917642 0.0100774901409161 -0.989014950817131 9.56372271996119e-05 PITA;miRNATAP -0.263078697018488 0.00030444275763597 NA NA NA hsa-miR-590-3p CDK6 1.73212597984247 2.26504285146779e-20 -0.989014950817131 9.56372271996119e-05 mirMAP;miRNATAP -0.317178870502969 2.42002063476642e-07 NA NA NA hsa-miR-590-5p CDK6 1.45684002317639 1.74263571329047e-15 -0.989014950817131 9.56372271996119e-05 mirMAP;miRNATAP -0.273075207720014 1.86611895239155e-05 NA NA NA hsa-miR-616-5p CDK6 1.97053352577988 6.50616168455898e-16 -0.989014950817131 9.56372271996119e-05 mirMAP -0.142613066171563 0.00307394605562186 NA NA NA hsa-miR-671-5p CDK6 2.3716249856662 4.71138196574897e-33 -0.989014950817131 9.56372271996119e-05 PITA;miRNATAP -0.190456888825144 0.000766145427004292 NA NA NA hsa-miR-7-1-3p CDK6 1.83538862227934 3.8339472676544e-18 -0.989014950817131 9.56372271996119e-05 mirMAP -0.248016446018151 5.76942925876452e-06 NA NA NA hsa-miR-9-3p CDK6 2.20853188955728 2.21538083391586e-07 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.134408373071388 1.4101697804249e-06 NA NA NA hsa-miR-9-5p CDK6 2.38651876502552 5.41791811050795e-08 -0.989014950817131 9.56372271996119e-05 mirMAP -0.118886025122029 9.78520255241092e-06 NA NA NA hsa-miR-92b-3p CDK6 2.30712626248952 3.58511983194853e-19 -0.989014950817131 9.56372271996119e-05 miRNATAP -0.266242569105257 1.75951619875479e-09 NA NA NA hsa-miR-93-5p CDK6 2.57501728456111 3.73950852324834e-39 -0.989014950817131 9.56372271996119e-05 mirMAP -0.153235126457442 0.00646254007007461 NA NA NA hsa-let-7e-5p CDKN1A 0.821771857232799 1.19334423679144e-10 -0.0493991502302471 0.848641722689714 MirTarget -0.25399591819006 0.0077168847872901 NA NA NA hsa-miR-101-3p CDKN1A -1.48434639686706 2.22407930927359e-19 -0.0493991502302471 0.848641722689714 MirTarget -0.321617945298423 6.01298813196538e-06 NA NA NA hsa-miR-106b-5p CDKN1A 2.17788294729604 3.35797658884941e-37 -0.0493991502302471 0.848641722689714 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.326259006114549 6.04540665162918e-07 NA NA NA hsa-miR-146b-5p CDKN1A 1.31285313891779 3.27846083137877e-10 -0.0493991502302471 0.848641722689714 miRNAWalker2_validate -0.203558425641458 0.000392548683760706 27602131 During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis progression thyroid cancer hsa-miR-17-5p CDKN1A 2.26819952852251 1.17080117367962e-29 -0.0493991502302471 0.848641722689714 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.343374853778203 1.00981087779429e-09 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-20a-5p CDKN1A 1.51276418035651 8.71307033346922e-13 -0.0493991502302471 0.848641722689714 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.38895427767553 1.75284776561439e-12 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-224-5p CDKN1A 1.54698256827631 5.96883056579208e-06 -0.0493991502302471 0.848641722689714 MirTarget -0.171545460457996 1.01742912316846e-06 26634414 Interestingly cell-unique downregulation of miR-224 or upregulation of miR-215 was found associated with CDKN1A induction colorectal cancer hsa-miR-28-5p CDKN1A -0.466180213899448 0.00012482099258297 -0.0493991502302471 0.848641722689714 miRNAWalker2_validate;miRTarBase;MirTarget;miRanda;miRNATAP -0.255606180200995 0.0121475686298841 NA NA NA hsa-miR-708-5p CDKN1A 0.874503880976718 0.00443872986168755 -0.0493991502302471 0.848641722689714 MirTarget -0.142688028712362 0.000308927269555815 NA NA NA hsa-miR-93-5p CDKN1A 2.57501728456111 3.73950852324834e-39 -0.0493991502302471 0.848641722689714 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.208998422262351 0.000231625937229055 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-10b-5p CDKN2A -1.71808089912719 3.75291415380712e-14 3.92273971998965 1.91639985434685e-20 miRNAWalker2_validate;miRTarBase -0.400100310739101 7.4963964454605e-06 NA NA NA hsa-miR-34a-5p CDKN2A 0.944430336151223 7.7422387648004e-06 3.92273971998965 1.91639985434685e-20 miRNAWalker2_validate -0.247785935180052 0.0122210036842052 26734589;25862914 The Significance of miR 34a Expression in Endometrial Carcinogenesis: Correlation With Expression of p16 and Ki 67 Proteins in Endometrial Cancers; This study was undertaken to analyze miR-34a expression in simple endometrial hyperplasia and endometrial cancer and to evaluate the relationship between expression of miR-34a and p16 and Ki-67 proteins in endometrial cancers; These were analyzed for miR-34a expression by quantitative real-time PCR and the expression of p16 and Ki-67 proteins in endometrial cancers was evaluated by immunohistochemistry; Although not statistically significant the frequency of p16 and Ki-67 overexpression tended to be lower in the cases with miR-34a underexpression than in cases with miR-34a overexpression;MiR-34a tumor levels significantly correlated with oropharyngeal origin p=0.0284 and p16 positivity p=0.0218 tumorigenesis; endometrial cancer;head and neck cancer hsa-miR-139-5p CHEK1 -2.41416537859302 6.88706484174732e-28 1.69355141540653 1.81819698771324e-25 miRanda -0.212366143767598 4.7377029023222e-10 NA NA NA hsa-miR-195-5p CHEK1 -1.81969168340723 1.04765314267131e-11 1.69355141540653 1.81819698771324e-25 MirTarget;miRNATAP -0.226721022215793 3.69949980552638e-15 25840419 MiR 195 suppresses non small cell lung cancer by targeting CHEK1; We discovered that CHEK1 was a direct target of miR-195 which decreased CHEK1 expression in lung cancer cells lung squamous cell cancer hsa-miR-424-5p CHEK1 -2.23160783176865 1.2307369299943e-15 1.69355141540653 1.81819698771324e-25 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.164917924156774 2.94933477208587e-09 22469983 Suppressed miR 424 expression via upregulation of target gene Chk1 contributes to the progression of cervical cancer; Moreover miR-424 expression levels were inversely correlated with Chk1 and p-Chk1 protein levels in both cervical cancer and normal tissues; Furthermore RNAi-mediated knockdown of Chk1 decreased matrix metalloproteinase 9 expression and phenocopied the tumor suppressive effects of miR-424 in cell models; Taken together our results identify a crucial tumor suppressive role of miR-424 in the progression of cervical cancer at least partly via upreglating the expression of Chk1 and p-Chk1 and suggest that miR-424 might be a candidate of prognostic predictor or an anticancer therapeutic target for cervical cancer patients progression cervical and endocervical cancer hsa-miR-497-5p CHEK1 -0.944286652785117 0.000311488624920066 1.69355141540653 1.81819698771324e-25 MirTarget;miRNATAP -0.159632825883805 1.81934427308075e-07 24464213 Checkpoint kinase 1 is negatively regulated by miR 497 in hepatocellular carcinoma; In silico analysis showed that CHEK1 was a candidate target of miR-497 which was previously found to be downregulated in HCC by us; To test whether miR-497 could bind to 3'untranslated region 3'UTR of CHEK1 luciferase reporter assay was conducted; The result revealed that miR-497 could bind to the 3'untranslated region 3'UTR of CHEK1 mRNA; Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1; Finally miR-497 expression was measured in the same 30 HCC samples and the correlation between miR-497 and CHEK1 was analyzed; The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1; Taken together these results demonstrated that CHEK1 was negatively regulated by miR-497 and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC which provided a potential molecular target for HCC therapy liver cancer hsa-miR-542-3p CHEK2 -2.02613612825205 8.22372377712865e-20 1.4039004664637 1.44057168008639e-21 miRanda -0.109163731915257 0.000511726642341551 NA NA NA hsa-miR-140-5p CYCS -0.887888681168421 5.76545563709515e-08 0.607605124707894 4.71796320922119e-06 miRanda -0.238330717985503 2.73731068681005e-10 NA NA NA hsa-miR-106a-5p FAS 2.93836356285531 1.22956096158575e-21 -1.05687386194019 7.55208132204358e-08 miRNAWalker2_validate;miRTarBase -0.18999823845935 3.151634810423e-11 22431000;27142596 miR 106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS; Bioinformatic analysis combining with validation experiments identified FAS as a direct target of miR-106a; Moreover a significant inverse correlation was found between miR-106a and FAS expression not only in gastric cancer cell lines but also in gastric cancer specimens; Taken together these findings suggest that ectopicly overexpressed miR-106a may play an oncogenic role in gastric carcinogenesis and impair extrinsic apoptotic pathway through targeting FAS;Functional experiment ascertained that miR-106a interacted with FAS and mediated caspase3 pathway tumorigenesis; gastric cancer;gastric cancer hsa-miR-361-5p FAS 0.505614361096266 0.000106749108296893 -1.05687386194019 7.55208132204358e-08 miRanda -0.324709656197576 7.30285975020992e-06 NA NA NA hsa-miR-491-5p FAS 1.22779907098202 1.78415304404366e-09 -1.05687386194019 7.55208132204358e-08 miRanda -0.110094371566109 0.0160189663610756 NA NA NA hsa-miR-590-5p FAS 1.45684002317639 1.74263571329047e-15 -1.05687386194019 7.55208132204358e-08 miRanda -0.289328057507152 4.6051084259043e-09 NA NA NA hsa-miR-98-5p FAS 1.05654915824416 2.25069999789746e-15 -1.05687386194019 7.55208132204358e-08 miRNAWalker2_validate -0.515907477977624 2.04225559395848e-14 NA NA NA hsa-miR-130a-3p GADD45A 0.188483151884821 0.402581058443795 -0.514765369988608 0.00492090888103211 MirTarget;miRNATAP -0.123561792128504 0.00138876699553693 NA NA NA hsa-miR-130b-3p GADD45A 3.42039109436067 3.89459455628164e-49 -0.514765369988608 0.00492090888103211 MirTarget;miRNATAP -0.101678408557166 0.00229464606464798 NA NA NA hsa-miR-146b-5p GADD45A 1.31285313891779 3.27846083137877e-10 -0.514765369988608 0.00492090888103211 miRanda -0.155377579461218 0.000135103789971723 NA NA NA hsa-miR-148b-3p GADD45A 1.80941322303736 4.89099431390024e-33 -0.514765369988608 0.00492090888103211 MirTarget -0.212358974333662 7.06101207590521e-05 NA NA NA hsa-miR-301a-3p GADD45A 1.9416887079489 3.80655048022936e-21 -0.514765369988608 0.00492090888103211 MirTarget;miRNATAP -0.178800608560708 8.44039970372651e-06 NA NA NA hsa-miR-324-5p GADD45A 2.14595874800649 3.03958987970622e-28 -0.514765369988608 0.00492090888103211 miRanda -0.124079317526865 0.0031120981024567 NA NA NA hsa-miR-331-5p GADD45A 1.35722430593336 4.30006931857061e-16 -0.514765369988608 0.00492090888103211 PITA;miRNATAP -0.164896174023222 0.00109272643171551 NA NA NA hsa-miR-361-3p GADD45A 0.701870987402474 2.37104651780238e-06 -0.514765369988608 0.00492090888103211 MirTarget -0.17834154236282 0.00222434435667187 NA NA NA hsa-miR-454-3p GADD45A 1.52878971973824 1.20025123958525e-19 -0.514765369988608 0.00492090888103211 MirTarget;miRNATAP -0.166134630615576 0.000797018564757013 NA NA NA hsa-miR-590-3p GADD45A 1.73212597984247 2.26504285146779e-20 -0.514765369988608 0.00492090888103211 miRanda;mirMAP -0.119961671181751 0.00717685047626542 NA NA NA hsa-miR-324-3p GADD45B 2.34606663412817 2.38165582241626e-31 -0.930432893111638 3.60855425958091e-06 MirTarget;miRNATAP -0.242037208710769 3.56306129694538e-08 NA NA NA hsa-miR-590-3p GADD45B 1.73212597984247 2.26504285146779e-20 -0.930432893111638 3.60855425958091e-06 miRanda -0.154523468408853 0.00171237179529765 NA NA NA hsa-miR-107 GADD45G 1.80682322226628 2.47002735902976e-27 -1.58665821115547 7.39273420165814e-10 miRanda -0.441303225004247 1.81725826941954e-10 NA NA NA hsa-miR-1976 GADD45G 2.88535661397958 1.06588775996244e-47 -1.58665821115547 7.39273420165814e-10 MirTarget -0.197073610405737 0.000529556407148933 NA NA NA hsa-miR-296-5p GADD45G -0.208779469897537 0.549226448418253 -1.58665821115547 7.39273420165814e-10 miRNATAP -0.107754085324882 0.00310257374946875 NA NA NA hsa-miR-320b GADD45G 1.09552613515995 1.0755423833529e-07 -1.58665821115547 7.39273420165814e-10 miRanda -0.145231743313527 0.0151063677392709 NA NA NA hsa-miR-421 GADD45G 2.10188043858202 8.21949037851434e-21 -1.58665821115547 7.39273420165814e-10 miRanda -0.226131038534135 1.95074577847569e-05 NA NA NA hsa-miR-429 GADD45G 5.03984117897747 4.44936205318976e-55 -1.58665821115547 7.39273420165814e-10 miRNATAP -0.105930203187737 0.0017440662666851 NA NA NA hsa-miR-139-5p GTSE1 -2.41416537859302 6.88706484174732e-28 4.28928649337725 2.35321123506919e-74 miRanda -0.500856783827883 2.06448697872002e-20 NA NA NA hsa-miR-140-5p GTSE1 -0.887888681168421 5.76545563709515e-08 4.28928649337725 2.35321123506919e-74 miRanda -0.34101869559629 2.00958280292284e-05 NA NA NA hsa-miR-455-5p GTSE1 -0.597175492609218 0.00643538336144091 4.28928649337725 2.35321123506919e-74 miRanda -0.144592110583326 0.0169766209713851 NA NA NA hsa-let-7f-1-3p IGF1 0.993720810761227 6.0407149976796e-08 -2.14280492881697 2.59941791149685e-09 mirMAP -0.290402698342035 0.00203057424337829 NA NA NA hsa-miR-103a-2-5p IGF1 2.42870001778613 1.23381577013723e-24 -2.14280492881697 2.59941791149685e-09 mirMAP -0.251547791163423 0.000314295644016659 NA NA NA hsa-miR-130b-3p IGF1 3.42039109436067 3.89459455628164e-49 -2.14280492881697 2.59941791149685e-09 MirTarget -0.269934586815518 4.77868913261969e-05 NA NA NA hsa-miR-148a-5p IGF1 1.24770308013998 1.92262031545036e-06 -2.14280492881697 2.59941791149685e-09 mirMAP -0.229831800276816 0.00043986574296279 NA NA NA hsa-miR-15b-3p IGF1 3.24791797249066 5.10037250255355e-48 -2.14280492881697 2.59941791149685e-09 mirMAP -0.390436257918866 1.35380667974337e-08 NA NA NA hsa-miR-16-1-3p IGF1 2.08698516922856 1.42419622445015e-24 -2.14280492881697 2.59941791149685e-09 mirMAP -0.248418763503828 0.00209215846343581 NA NA NA hsa-miR-19a-3p IGF1 1.84830198357993 2.4710712042826e-20 -2.14280492881697 2.59941791149685e-09 MirTarget -0.265285654505214 0.00141037816559179 NA NA NA hsa-miR-19b-1-5p IGF1 0.959999569197417 4.46371760817159e-06 -2.14280492881697 2.59941791149685e-09 mirMAP -0.330665708721346 5.64355959062011e-05 NA NA NA hsa-miR-27a-3p IGF1 1.20835958303386 1.23147423005989e-12 -2.14280492881697 2.59941791149685e-09 miRNAWalker2_validate;miRTarBase -0.32251985768723 0.00124209665002292 NA NA NA hsa-miR-301a-3p IGF1 1.9416887079489 3.80655048022936e-21 -2.14280492881697 2.59941791149685e-09 MirTarget -0.225476040760445 0.00526108072368388 NA NA NA hsa-miR-3065-5p IGF1 2.75496955380748 2.37091362562179e-15 -2.14280492881697 2.59941791149685e-09 mirMAP -0.185273914068493 0.000247699335715572 NA NA NA hsa-miR-32-3p IGF1 2.40467177052961 5.81388257063569e-26 -2.14280492881697 2.59941791149685e-09 mirMAP -0.191482365278494 0.0131051550839598 NA NA NA hsa-miR-33a-3p IGF1 1.39444251212239 7.26586547385458e-13 -2.14280492881697 2.59941791149685e-09 MirTarget -0.23268819154373 0.00800410476456397 NA NA NA hsa-miR-486-5p IGF1 0.630265651169473 0.0356088002128703 -2.14280492881697 2.59941791149685e-09 PITA;miRNATAP -0.196250517167786 0.000667616783745863 NA NA NA hsa-miR-576-5p IGF1 1.30779208544019 1.20419097314618e-13 -2.14280492881697 2.59941791149685e-09 PITA;mirMAP;miRNATAP -0.219281493796161 0.022843578496806 NA NA NA hsa-miR-590-3p IGF1 1.73212597984247 2.26504285146779e-20 -2.14280492881697 2.59941791149685e-09 MirTarget;miRanda;mirMAP;miRNATAP -0.240935178114737 0.00690487234405161 NA NA NA hsa-miR-592 IGF1 3.42176893366567 4.25404501506769e-21 -2.14280492881697 2.59941791149685e-09 mirMAP -0.129442645000446 0.00826936526891666 NA NA NA hsa-miR-629-5p IGF1 1.40922412417551 5.33576724423842e-10 -2.14280492881697 2.59941791149685e-09 mirMAP -0.264359104457603 0.000419052275131738 NA NA NA hsa-miR-940 IGF1 3.48873734617979 1.86622896910559e-36 -2.14280492881697 2.59941791149685e-09 MirTarget;PITA;miRNATAP -0.191770271380563 0.00124823224951527 NA NA NA hsa-let-7a-3p IGFBP3 1.03029456575871 2.05932484675687e-08 -0.311531996914283 0.18234552189749 miRNATAP -0.197619943951327 0.000878211481366828 NA NA NA hsa-miR-197-3p IGFBP3 1.87571028261536 4.68641627630274e-27 -0.311531996914283 0.18234552189749 MirTarget;miRNATAP -0.252940616530171 2.22421317195562e-05 NA NA NA hsa-miR-19a-3p IGFBP3 1.84830198357993 2.4710712042826e-20 -0.311531996914283 0.18234552189749 MirTarget;miRNATAP -0.261172824642014 6.44213826936234e-07 NA NA NA hsa-miR-19b-3p IGFBP3 1.34004947278979 5.26920324973452e-16 -0.311531996914283 0.18234552189749 MirTarget;miRNATAP -0.39878455783284 4.33990984278995e-10 NA NA NA hsa-miR-339-5p IGFBP3 1.79341790518551 3.04346641619717e-17 -0.311531996914283 0.18234552189749 miRanda -0.186478176187251 0.000196020741332572 NA NA NA hsa-miR-340-5p IGFBP3 1.35294088371205 8.25205106713644e-16 -0.311531996914283 0.18234552189749 MirTarget -0.217783370626594 0.000646296756021528 NA NA NA hsa-miR-374a-5p IGFBP3 -0.0832626833277432 0.509637142371946 -0.311531996914283 0.18234552189749 MirTarget;mirMAP -0.239562978609385 0.00719698130694237 NA NA NA hsa-miR-374b-5p IGFBP3 -0.162562360514506 0.226063855202319 -0.311531996914283 0.18234552189749 MirTarget -0.324687892189915 9.49219069071261e-05 NA NA NA hsa-miR-590-3p IGFBP3 1.73212597984247 2.26504285146779e-20 -0.311531996914283 0.18234552189749 miRanda -0.124354821718399 0.0287524247404911 NA NA NA hsa-miR-590-5p IGFBP3 1.45684002317639 1.74263571329047e-15 -0.311531996914283 0.18234552189749 miRanda -0.147694741932385 0.0119157386274042 NA NA NA hsa-miR-616-5p IGFBP3 1.97053352577988 6.50616168455898e-16 -0.311531996914283 0.18234552189749 MirTarget -0.15147207626533 0.000572221690537221 NA NA NA hsa-miR-21-5p MDM4 1.51489362035879 4.99155235011048e-20 -0.0103627395302235 0.931442844424316 miRNAWalker2_validate;mirMAP -0.124676826896648 0.000153768081514085 NA NA NA hsa-miR-27a-3p MDM4 1.20835958303386 1.23147423005989e-12 -0.0103627395302235 0.931442844424316 miRNATAP -0.129914185524538 6.59590744066317e-05 NA NA NA hsa-miR-330-5p MDM4 2.22118657747194 1.60999465282299e-26 -0.0103627395302235 0.931442844424316 miRanda -0.101096504897684 7.77328369428359e-05 NA NA NA hsa-miR-125a-3p PERP 0.766248892008454 5.97048811423325e-05 1.01267004811397 2.43673857373096e-07 miRanda -0.113304540490232 0.0214841048818742 NA NA NA hsa-miR-126-5p PERP -0.593422896912062 3.02290704755607e-05 1.01267004811397 2.43673857373096e-07 mirMAP -0.14784456139744 0.0261629554944329 NA NA NA hsa-miR-146b-5p PERP 1.31285313891779 3.27846083137877e-10 1.01267004811397 2.43673857373096e-07 miRanda -0.102865127629662 0.0203015230664685 NA NA NA hsa-miR-29b-1-5p PERP 0.186982580224589 0.373497464809179 1.01267004811397 2.43673857373096e-07 MirTarget -0.116443584831033 0.0098451764390626 NA NA NA hsa-miR-361-5p PERP 0.505614361096266 0.000106749108296893 1.01267004811397 2.43673857373096e-07 miRanda -0.186809501605129 0.0102092566329975 NA NA NA hsa-let-7a-3p PPM1D 1.03029456575871 2.05932484675687e-08 -0.83070665662455 9.29448428990069e-12 MirTarget -0.186173347634636 2.56426809291406e-09 NA NA NA hsa-let-7b-3p PPM1D 0.348643846906648 0.0705621714966111 -0.83070665662455 9.29448428990069e-12 MirTarget -0.106042141884383 0.000538066224251497 NA NA NA hsa-let-7f-1-3p PPM1D 0.993720810761227 6.0407149976796e-08 -0.83070665662455 9.29448428990069e-12 MirTarget -0.143680161944435 6.08025088960724e-06 NA NA NA hsa-miR-141-5p PPM1D 4.1163822626281 1.95594003811718e-46 -0.83070665662455 9.29448428990069e-12 MirTarget -0.139002371873183 6.3908667157681e-15 NA NA NA hsa-miR-148b-5p PPM1D 2.24566117508049 6.39491669468652e-28 -0.83070665662455 9.29448428990069e-12 MirTarget -0.168581857409081 2.39001470308872e-10 NA NA NA hsa-miR-15a-5p PPM1D 1.77791080875961 1.15367449842886e-31 -0.83070665662455 9.29448428990069e-12 MirTarget;miRNATAP -0.155762135264756 1.65057654998281e-05 NA NA NA hsa-miR-15b-5p PPM1D 2.49680902955696 1.79560488884714e-45 -0.83070665662455 9.29448428990069e-12 MirTarget;miRNATAP -0.17212105804611 7.19813823162557e-09 NA NA NA hsa-miR-16-5p PPM1D 1.88120757008208 1.6207082610779e-32 -0.83070665662455 9.29448428990069e-12 miRNAWalker2_validate;MirTarget;miRNATAP -0.205999884465744 1.72151969939106e-09 26064212 The relative activity of renila luciferase was detected to confirm the binding site of mir-16 on wip1 mRNA; Transfection of mir-16 minics significantly suppressed the luciferase reporter containing wild type not mutant wip1 3'-UTR lung cancer hsa-miR-26b-5p PPM1D 0.300594892480765 0.0431916558729258 -0.83070665662455 9.29448428990069e-12 miRNAWalker2_validate -0.212432394557768 7.98010193309949e-08 NA NA NA hsa-miR-32-3p PPM1D 2.40467177052961 5.81388257063569e-26 -0.83070665662455 9.29448428990069e-12 miRNATAP -0.165695519066186 1.12563770509835e-10 NA NA NA hsa-miR-32-5p PPM1D 1.61585199704519 4.93530744182272e-20 -0.83070665662455 9.29448428990069e-12 MirTarget -0.130712700204754 4.48544787196286e-05 NA NA NA hsa-miR-320a PPM1D 0.502704491334741 0.00226252073794899 -0.83070665662455 9.29448428990069e-12 miRanda -0.159483796000997 7.97968469380669e-06 NA NA NA hsa-miR-320b PPM1D 1.09552613515995 1.0755423833529e-07 -0.83070665662455 9.29448428990069e-12 miRanda -0.101231046802467 0.000331807967752927 NA NA NA hsa-miR-320c PPM1D 0.733362580458909 0.00057735467707277 -0.83070665662455 9.29448428990069e-12 miRanda -0.116682955417797 3.331197058659e-05 NA NA NA hsa-miR-33a-3p PPM1D 1.39444251212239 7.26586547385458e-13 -0.83070665662455 9.29448428990069e-12 MirTarget -0.140346184241498 2.01803895607381e-06 NA NA NA hsa-miR-429 PPM1D 5.03984117897747 4.44936205318976e-55 -0.83070665662455 9.29448428990069e-12 miRanda -0.118424088855251 3.24641576503372e-14 NA NA NA hsa-miR-532-3p PPM1D 1.39711438989287 4.70050755096321e-15 -0.83070665662455 9.29448428990069e-12 MirTarget -0.144259462339518 6.34998293435089e-06 NA NA NA hsa-miR-590-3p PPM1D 1.73212597984247 2.26504285146779e-20 -0.83070665662455 9.29448428990069e-12 MirTarget;PITA;miRanda;mirMAP -0.151850904049414 4.06589999109405e-07 NA NA NA hsa-miR-590-5p PPM1D 1.45684002317639 1.74263571329047e-15 -0.83070665662455 9.29448428990069e-12 miRanda -0.149950023838726 1.34706033863153e-06 NA NA NA hsa-let-7f-1-3p PTEN 0.993720810761227 6.0407149976796e-08 -0.84427149621286 2.56858517759026e-07 mirMAP -0.127233658331135 0.00280961062498823 NA NA NA hsa-miR-103a-3p PTEN 1.51214214201065 3.45388560860799e-21 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase -0.183182958091902 9.52119451919609e-05 26511107;24828205 LncRNA GAS5 induces PTEN expression through inhibiting miR 103 in endometrial cancer cells; To investigate the expression of GAS5 PTEN and miR-103 RT-PCR was performed; Finally we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells; Through inhibiting the expression of miR-103 GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis and thus could be an important mediator in the pathogenesis of endometrial cancer;Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN ; endometrial cancer;colorectal cancer hsa-miR-106a-5p PTEN 2.93836356285531 1.22956096158575e-21 -0.84427149621286 2.56858517759026e-07 miRNATAP -0.138543913825139 7.29385022821716e-09 26097565;26318586 miR 106a promotes growth and metastasis of non small cell lung cancer by targeting PTEN; Furthermore the presence of miR-106a was inversely correlated with PTEN in NSCLC tissues; Overall this study suggested that miR-106a inhibited the growth and metastasis of NSCLC cells by decreasing PTEN expression;Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo metastasis; lung squamous cell cancer;prostate cancer hsa-miR-106b-5p PTEN 2.17788294729604 3.35797658884941e-37 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;miRNATAP -0.250524474225071 1.87549794636595e-09 24842611;26238857;26722252 MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance;Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells cell migration;drug resistance; gastric cancer;colorectal cancer;breast cancer hsa-miR-107 PTEN 1.80682322226628 2.47002735902976e-27 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;PITA;miRanda -0.181352170275222 4.18635576468689e-05 NA NA NA hsa-miR-125a-3p PTEN 0.766248892008454 5.97048811423325e-05 -0.84427149621286 2.56858517759026e-07 miRanda -0.111729935890432 0.00653528699979761 NA NA NA hsa-miR-130b-3p PTEN 3.42039109436067 3.89459455628164e-49 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.156206688418345 1.6593540482809e-07 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-141-3p PTEN 4.68123467996176 4.92783859837668e-50 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.100784910378171 4.61079942988718e-06 24742567;27644195 PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis;Involvement of microRNA 141 3p in 5 fluorouracil and oxaliplatin chemo resistance in esophageal cancer cells via regulation of PTEN; Western blot exhibited altered protein levels of PTEN Akt and PI3k with miR-141-3p inhibitor; An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples; Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo tumorigenesis;drug resistance endometrial cancer;esophageal cancer hsa-miR-148b-3p PTEN 1.80941322303736 4.89099431390024e-33 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.216617867712398 6.96745047085722e-06 NA NA NA hsa-miR-15b-3p PTEN 3.24791797249066 5.10037250255355e-48 -0.84427149621286 2.56858517759026e-07 mirMAP -0.206513771757453 2.27250245840195e-11 NA NA NA hsa-miR-16-1-3p PTEN 2.08698516922856 1.42419622445015e-24 -0.84427149621286 2.56858517759026e-07 MirTarget -0.158720233662343 1.24391775503953e-05 NA NA NA hsa-miR-16-2-3p PTEN 2.33811104285535 7.35637935757058e-33 -0.84427149621286 2.56858517759026e-07 mirMAP -0.198566847333123 7.27397127510284e-08 NA NA NA hsa-miR-17-5p PTEN 2.26819952852251 1.17080117367962e-29 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.173820297208016 1.76091146389354e-06 27400681;23391506;23133552;26629823;24462867;26318586;26215320;25634356;26500892;24912422;23418359 GFRα2 prompts cell growth and chemoresistance through down regulating tumor suppressor gene PTEN via Mir 17 5p in pancreatic cancer; Mechanically we discovered that high GFRα2 expression level leads to PTEN inactivation via enhancing Mir-17-5p level;We found that these phenotypes were the results of miR-17 targeting PTEN;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;We hypothesized that knocking down the oncogenic microRNA oncomiR miR-17-5p might restore the expression levels of PDCD4 and PTEN tumor suppressor proteins illustrating a route to oligonucleotide therapy of TNBC; Contrary to conventional wisdom antisense knockdown of oncomiR miR-17-5p guide strand reduced PDCD4 and PTEN proteins by 1.8±0.3 fold in human TNBC cells instead of raising them; Bioinformatics analysis and folding energy calculations revealed that mRNA targets of miR-17-5p guide strand such as PDCD4 and PTEN could also be regulated by miR-17-3p passenger strand;miR 17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression; Expression of miR-17 was negatively correlated with PTEN in OS tissues;Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR 17 family in prostate cancer; Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo;In addition ERβ expression significantly increased in calycosin-treated HCT-116 cells followed by a decrease of miR-17 and up-regulation of PTEN; Our results indicate that calycosin has an inhibitory effect on CRC which might be obtained by ERβ-mediated regulation of miR-17 and PTEN expression;The High Expression of the microRNA 17 92 Cluster and its Paralogs and the Downregulation of the Target Gene PTEN Is Associated with Primary Cutaneous B Cell Lymphoma Progression;MicroRNA 17 5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling; miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells;MicroRNA 17 5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression; We found that PTEN was a target of miR-17-5p in the colon cancer cells and their context-specific interactions were responsible for multiple drug-resistance; Chemotherapy was found to increase the expression levels of miR-17-5p which further repressed PTEN levels contributing to the development of chemo-resistance; MiR-17-5p is a predictive factor for chemotherapy response and a prognostic factor for overall survival in CRC which is due to its regulation of PTEN expression;The mature miR-17-5p exerted this function by repressing the expression of PTEN drug resistance;;;;metastasis;;;progression;drug resistance;metastasis;drug resistance;poor survival; pancreatic cancer;glioblastoma;sarcoma;breast cancer;sarcoma;prostate cancer;colorectal cancer;B cell lymphoma;ovarian cancer;colorectal cancer;liver cancer hsa-miR-182-5p PTEN 5.17636717835373 1.04663621342014e-76 -0.84427149621286 2.56858517759026e-07 mirMAP -0.11933905629008 2.87742676309259e-07 NA NA NA hsa-miR-186-5p PTEN 1.01160002003511 1.0995153919986e-13 -0.84427149621286 2.56858517759026e-07 mirMAP;miRNATAP -0.279158600178463 6.74558938940574e-07 NA NA NA hsa-miR-188-5p PTEN 1.83180846049089 3.69593490679723e-16 -0.84427149621286 2.56858517759026e-07 MirTarget;PITA;miRNATAP -0.162055696030893 1.38452463430442e-06 NA NA NA hsa-miR-18a-5p PTEN 3.22282140470889 1.9203457351048e-39 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase -0.166371378968616 7.91650990648861e-09 24681249;27291152 However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis;miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis metastasis; colon cancer;esophageal cancer hsa-miR-193b-3p PTEN 1.19715471134498 1.60271373616177e-07 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRNATAP -0.135208099074967 6.37609081516721e-05 NA NA NA hsa-miR-19a-3p PTEN 1.84830198357993 2.4710712042826e-20 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.171238699524273 4.56798718178587e-06 27445062;26098000;24831732;21853360;24681249;27289489;25107371 The target genes of miR-19a such as ABCA1 and PTEN that had been suppressed by miR recovered their expression through CAP treatment;Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN;Transfection of miR-19a and -19b mimics reversed the up-regulations of IGF2R and PTEN gene expression and abrogated the GSE induced anti-proliferative response;The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid; Meanwhile gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN; The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN ;;;drug resistance;metastasis;drug resistance; breast cancer;lung cancer;breast cancer;breast cancer;colon cancer;lung cancer;bladder cancer hsa-miR-19b-3p PTEN 1.34004947278979 5.26920324973452e-16 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.196134872344623 1.94906356585214e-05 26098000;24831732;21853360;24681249 Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN ;;drug resistance;metastasis lung cancer;breast cancer;breast cancer;colon cancer hsa-miR-200c-3p PTEN 4.19740435353621 6.07734417092297e-49 -0.84427149621286 2.56858517759026e-07 mirMAP;miRNATAP -0.101506195158424 3.13425564253727e-05 24682933;22637745 In conclusion miR-200c functions as an oncogene in colon cancer cells through regulating tumor cell apoptosis survival invasion and metastasis as well as xenograft tumor growth through inhibition of PTEN expression and p53 phosphorylation;Forced over-expression or silencing of miR-200c followed by either CDF or BR-DIM treatment of MIAPaCa-2 cells altered the morphology of cells wound-healing capacity colony formation and the expression of MT1-MMP and PTEN; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment metastasis;poor survival; colon cancer;pancreatic cancer hsa-miR-20a-5p PTEN 1.51276418035651 8.71307033346922e-13 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;miRNATAP -0.12902418362165 0.000355771789438909 26031366 The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction; MiR-20a levels were increased in HCC cell lines and tissues whereas PTEN was inversely correlated with it; PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance drug resistance liver cancer hsa-miR-23a-3p PTEN 1.0834199821542 3.34616494480896e-13 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.132173719788307 0.0108182358011119 24317043;26160225 Taken together our findings suggest that ACA might have anticancer effects against human head and neck cancer through downregulation of miR-23a which can repress tumor suppressor PTEN;MicroRNA 23a enhances migration and invasion through PTEN in osteosarcoma; Gene and protein levels of miR-23a and PTEN were examined to determine the molecular relationship between them in the pathogenesis of osteosarcoma; Bioinformatics and luciferase-reporter assay revealed that miR-23a specifically targeted the 3'-untranslational region of PTEN and regulated its expression; Our study showed that miR-23a by downregulation of PTEN enhanced migration and invasion in osteosarcoma cells ; head and neck cancer;sarcoma hsa-miR-25-3p PTEN 1.36338550911959 2.27429614057655e-21 -0.84427149621286 2.56858517759026e-07 miRTarBase;MirTarget;miRNATAP -0.257676903343044 7.78945807324694e-07 NA NA NA hsa-miR-26b-5p PTEN 0.300594892480765 0.0431916558729258 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.108224803763522 0.0429141036715979 26068649 Down regulation of microRNA 26b modulates non small cell lung cancer cells chemoresistance and migration through the association of PTEN; It indicates that miR-26b may regulate NSCLC migration and chemosensitivity through the regulation of PTEN drug resistance lung squamous cell cancer hsa-miR-301a-3p PTEN 1.9416887079489 3.80655048022936e-21 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.190369213465919 1.38100003810051e-07 24315818;26846737 Upregulated microRNA 301a in breast cancer promotes tumor metastasis by targeting PTEN and activating Wnt/β catenin signaling; Furthermore miR-301a directly targeted and suppressed PTEN one negative regulator of the Wnt/β-catenin signaling cascade; These results demonstrate that miR-301a maintains constitutively activated Wnt/β-catenin signaling by directly targeting PTEN which promotes breast cancer invasion and metastasis;MicroRNA 301a promotes cell proliferation via PTEN targeting in Ewing's sarcoma cells; Our results demonstrated the novel mechanism controlling PTEN expression via miR-301a in ES cells metastasis; breast cancer;sarcoma hsa-miR-30b-5p PTEN 0.359198073795339 0.0288588301689338 -0.84427149621286 2.56858517759026e-07 mirMAP -0.131287666012627 0.00635778274696439 NA NA NA hsa-miR-30d-3p PTEN 0.755916398923689 3.64952022092868e-05 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.121268670790555 0.00463337748589533 NA NA NA hsa-miR-30e-5p PTEN 0.264119434405151 0.0488892309487705 -0.84427149621286 2.56858517759026e-07 mirMAP -0.138582594470504 0.0197094509446128 NA NA NA hsa-miR-32-3p PTEN 2.40467177052961 5.81388257063569e-26 -0.84427149621286 2.56858517759026e-07 mirMAP -0.140427212052649 5.21899744853115e-05 24123284;25647261;23617834 In this study we determined the levels of the correlation between and the clinical significance of the expression of miR-32 and phosphatase and tensin homologue PTEN a tumor suppressor targeted by miR-32 in CRC; The levels of miR-32 and PTEN gene expression in 35 colorectal carcinoma samples 35 corresponding cancer-adjacent tissue samples 27 colorectal adenoma samples and 16 normal tissue samples were quantified using real-time quantitative reverse transcriptase-polymerase chain reaction; The relationship between the miR-32 and PTEN protein expression and clinicopathological factors was analyzed; Significant upregulation of miR-32 expression and reduction of PTEN were identified in CRC tissues; An inverse relationship between miR-32 and PTEN protein expression was identified; MiR-32 and PTEN expression were inversely correlated and miR-32 may be associated with the development of CRC;MiR 32 induces cell proliferation migration and invasion in hepatocellular carcinoma by targeting PTEN; Besides miRNA-32 down-regulates PTEN through binding to 3'-UTR of PTEN mRNA from luciferase reporter assay and the expression level of miR-32 could affect the proliferation migration and invasion of liver cancer cell lines via PTEN/Akt signaling pathway; Down-expression of PTEN could significantly attenuate the inhibitory effects of knockdown miR-32 on the proliferation migration and invasion of liver cancer cells suggesting that miR-32 could be a potential target for HCC treatment;MicroRNA 32 miR 32 regulates phosphatase and tensin homologue PTEN expression and promotes growth migration and invasion in colorectal carcinoma cells; In this study we identified the potential effects of miR-32 on some important biological properties of CRC cells and clarified the regulation of PTEN by miR-32; The 3'-untranslated region 3'-UTR of PTEN combined with miR-32 was verified by dual-luciferase reporter assay; Gain-of-function and loss-of-function studies showed that overexpression of miR-32 promoted SW480 cell proliferation migration and invasion reduced apoptosis and resulted in downregulation of PTEN at a posttranscriptional level; However miR-32 knock-down inhibited these processes in HCT-116 cells and enhanced the expression of PTEN protein; In addition we further identified PTEN as the functional downstream target of miR-32 by directly targeting the 3'-UTR of PTEN; Our results demonstrated that miR-32 was involved in tumorigenesis of CRC at least in part by suppression of PTEN ;;tumorigenesis colorectal cancer;liver cancer;colorectal cancer hsa-miR-32-5p PTEN 1.61585199704519 4.93530744182272e-20 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.191091085245776 7.19634191579789e-06 24123284;25647261;23617834 In this study we determined the levels of the correlation between and the clinical significance of the expression of miR-32 and phosphatase and tensin homologue PTEN a tumor suppressor targeted by miR-32 in CRC; The levels of miR-32 and PTEN gene expression in 35 colorectal carcinoma samples 35 corresponding cancer-adjacent tissue samples 27 colorectal adenoma samples and 16 normal tissue samples were quantified using real-time quantitative reverse transcriptase-polymerase chain reaction; The relationship between the miR-32 and PTEN protein expression and clinicopathological factors was analyzed; Significant upregulation of miR-32 expression and reduction of PTEN were identified in CRC tissues; An inverse relationship between miR-32 and PTEN protein expression was identified; MiR-32 and PTEN expression were inversely correlated and miR-32 may be associated with the development of CRC;MiR 32 induces cell proliferation migration and invasion in hepatocellular carcinoma by targeting PTEN; Besides miRNA-32 down-regulates PTEN through binding to 3'-UTR of PTEN mRNA from luciferase reporter assay and the expression level of miR-32 could affect the proliferation migration and invasion of liver cancer cell lines via PTEN/Akt signaling pathway; Down-expression of PTEN could significantly attenuate the inhibitory effects of knockdown miR-32 on the proliferation migration and invasion of liver cancer cells suggesting that miR-32 could be a potential target for HCC treatment;MicroRNA 32 miR 32 regulates phosphatase and tensin homologue PTEN expression and promotes growth migration and invasion in colorectal carcinoma cells; In this study we identified the potential effects of miR-32 on some important biological properties of CRC cells and clarified the regulation of PTEN by miR-32; The 3'-untranslated region 3'-UTR of PTEN combined with miR-32 was verified by dual-luciferase reporter assay; Gain-of-function and loss-of-function studies showed that overexpression of miR-32 promoted SW480 cell proliferation migration and invasion reduced apoptosis and resulted in downregulation of PTEN at a posttranscriptional level; However miR-32 knock-down inhibited these processes in HCT-116 cells and enhanced the expression of PTEN protein; In addition we further identified PTEN as the functional downstream target of miR-32 by directly targeting the 3'-UTR of PTEN; Our results demonstrated that miR-32 was involved in tumorigenesis of CRC at least in part by suppression of PTEN ;;tumorigenesis colorectal cancer;liver cancer;colorectal cancer hsa-miR-320a PTEN 0.502704491334741 0.00226252073794899 -0.84427149621286 2.56858517759026e-07 MirTarget;PITA;miRanda;miRNATAP -0.179461519094388 0.000169707013533764 NA NA NA hsa-miR-320b PTEN 1.09552613515995 1.0755423833529e-07 -0.84427149621286 2.56858517759026e-07 MirTarget;PITA;miRanda;miRNATAP -0.165236028060939 1.01184739025079e-05 NA NA NA hsa-miR-320c PTEN 0.733362580458909 0.00057735467707277 -0.84427149621286 2.56858517759026e-07 MirTarget;PITA;miRanda;miRNATAP -0.115800831670003 0.00208219976527899 NA NA NA hsa-miR-330-3p PTEN 1.76420587112262 1.73634190017122e-17 -0.84427149621286 2.56858517759026e-07 mirMAP -0.128607364079954 0.000433361795764457 NA NA NA hsa-miR-335-3p PTEN 1.32116009044055 1.0694366259168e-09 -0.84427149621286 2.56858517759026e-07 MirTarget -0.155975685276752 1.06616962543212e-05 NA NA NA hsa-miR-340-5p PTEN 1.35294088371205 8.25205106713644e-16 -0.84427149621286 2.56858517759026e-07 miRNATAP -0.151622948618916 0.000831621350764217 NA NA NA hsa-miR-342-3p PTEN 0.909988426908683 3.51879055202261e-09 -0.84427149621286 2.56858517759026e-07 miRanda -0.208946346149697 3.2083861124117e-05 NA NA NA hsa-miR-374a-5p PTEN -0.0832626833277432 0.509637142371946 -0.84427149621286 2.56858517759026e-07 MirTarget;mirMAP -0.134110922196539 0.0344819869486353 NA NA NA hsa-miR-374b-5p PTEN -0.162562360514506 0.226063855202319 -0.84427149621286 2.56858517759026e-07 MirTarget;mirMAP;miRNATAP -0.159286891775142 0.00729676184303562 NA NA NA hsa-miR-421 PTEN 2.10188043858202 8.21949037851434e-21 -0.84427149621286 2.56858517759026e-07 miRanda -0.170368648587642 3.03031633209092e-07 NA NA NA hsa-miR-425-5p PTEN 3.37583546071413 8.59535002332206e-48 -0.84427149621286 2.56858517759026e-07 miRNATAP -0.166627633584347 2.34589119170066e-08 25154996 An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation gastric cancer hsa-miR-429 PTEN 5.03984117897747 4.44936205318976e-55 -0.84427149621286 2.56858517759026e-07 PITA;miRanda;mirMAP;miRNATAP -0.104315093958323 8.41440789886008e-07 NA NA NA hsa-miR-454-3p PTEN 1.52878971973824 1.20025123958525e-19 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.176655039809621 7.68859946739887e-05 26296312;27261580 MicroRNA 454 functions as an oncogene by regulating PTEN in uveal melanoma; Furthermore we identified PTEN as a direct target of miR-454; Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells;MiR 454 promotes the progression of human non small cell lung cancer and directly targets PTEN; At last the potential regulatory function of miR-454 on PTEN expression was confirmed; Further PTEN was confirmed as a direct target of miR-454 by using Luciferase Reporter Assay ;progression melanoma;lung squamous cell cancer hsa-miR-484 PTEN 2.63208825865802 1.33951432214396e-44 -0.84427149621286 2.56858517759026e-07 miRNATAP -0.148037989031567 8.34915055314783e-05 NA NA NA hsa-miR-589-3p PTEN 1.69712322956823 3.70306979263175e-12 -0.84427149621286 2.56858517759026e-07 MirTarget;mirMAP -0.11514248400618 0.00031786047004444 NA NA NA hsa-miR-590-3p PTEN 1.73212597984247 2.26504285146779e-20 -0.84427149621286 2.56858517759026e-07 MirTarget;PITA;miRanda;mirMAP -0.161997112996927 5.43800942124825e-05 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting; miR-590-3p was found to activate PI3K-AKT signaling pathway by down-regulating PTEN to promote AKT1-S473 phosphorylation liver cancer hsa-miR-590-5p PTEN 1.45684002317639 1.74263571329047e-15 -0.84427149621286 2.56858517759026e-07 mirMAP -0.229773231990624 2.35006940384485e-08 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-616-5p PTEN 1.97053352577988 6.50616168455898e-16 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.145907638722278 2.64048330609057e-06 NA NA NA hsa-miR-628-3p PTEN 0.967264135353398 1.20380730705551e-05 -0.84427149621286 2.56858517759026e-07 MirTarget;PITA -0.109572703221522 0.00206213911286011 NA NA NA hsa-miR-7-1-3p PTEN 1.83538862227934 3.8339472676544e-18 -0.84427149621286 2.56858517759026e-07 mirMAP -0.127391773118384 0.000357719306278926 NA NA NA hsa-miR-744-3p PTEN 2.11872740311414 3.16695678742758e-19 -0.84427149621286 2.56858517759026e-07 miRNATAP -0.161164161645532 3.23315243272093e-07 NA NA NA hsa-miR-92a-3p PTEN 1.69110879028255 8.04580501722806e-26 -0.84427149621286 2.56858517759026e-07 MirTarget;miRNATAP -0.187378955127214 4.8047502502359e-05 26432332;25515201;24137349;23546593;23133552;24026406 Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor; Taken together these findings suggested that miR-92a could promote growth metastasis and chemoresistance in NSCLC cells at least partially by targeting PTEN;MiR 92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN Mediated PI3K/AKT Pathway; The expression of miR-92a PTEN and E-cadherin was analyzed by real-time PCR; In addition there was a negative correlation between levels of miR-92a and the PTEN gene p < 0.0001; The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;The expression levels of miR-92a and phosphatase and tensin homologue PTEN were detected by qRT-PCR and western blot; In addition the regulation of PTEN by miR-92a was evaluated by qRT-PCR western blot and luciferase reporter assays; There was an inverse correlation between the levels of miR-92a and PTEN in CRC tissues; The overexpression of miR-92a in CRC cells decreased PTEN expression at the translational level and decreased PTEN-driven luciferase-reporter activity; Our results demonstrated that miR-92a induced EMT and regulated cell growth migration and invasion in the SW480 cells at least partially via suppression of PTEN expression metastasis;drug resistance;metastasis;cell migration;;worse prognosis;; lung squamous cell cancer;colorectal cancer;cervical and endocervical cancer;liver cancer;sarcoma;colorectal cancer hsa-miR-93-5p PTEN 2.57501728456111 3.73950852324834e-39 -0.84427149621286 2.56858517759026e-07 miRNAWalker2_validate;miRTarBase;miRNATAP -0.18244820649202 4.60583878947344e-07 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-424-5p RFWD2 -2.23160783176865 1.2307369299943e-15 0.547242266975046 3.11957227546194e-11 MirTarget;miRNATAP -0.121405375871416 2.7438332934687e-20 NA NA NA hsa-miR-338-3p RPRM 0.429940853175601 0.097699268368127 -4.67301758708093 3.22916998718183e-23 miRanda -0.233608437618068 0.0100340137640246 NA NA NA hsa-miR-423-5p RPRM 0.875722574439598 4.995026399727e-09 -4.67301758708093 3.22916998718183e-23 MirTarget -0.755461430856466 1.00596149308801e-06 NA NA NA hsa-miR-501-3p RPRM 2.18792856444299 6.3664407099549e-25 -4.67301758708093 3.22916998718183e-23 miRNATAP -0.625838178752399 1.36497991706476e-09 NA NA NA hsa-miR-940 RPRM 3.48873734617979 1.86622896910559e-36 -4.67301758708093 3.22916998718183e-23 miRNATAP -0.481321254541463 1.29178707875463e-09 NA NA NA hsa-miR-100-5p RRM2 -2.83787537179199 1.16422564671006e-21 4.63226118042495 4.42418159710687e-69 miRNAWalker2_validate -0.398646366482872 1.4243924093358e-18 NA NA NA hsa-miR-125a-5p RRM2 -0.347216996653838 0.0211699797450826 4.63226118042495 4.42418159710687e-69 miRanda -0.719948149506262 5.53693742606249e-14 NA NA NA hsa-miR-199a-5p RRM2 -0.898723308717733 0.000310515393412944 4.63226118042495 4.42418159710687e-69 miRanda -0.20308066451424 0.000523589436140355 NA NA NA hsa-miR-199b-5p RRM2 -0.530328679859984 0.0666759691762674 4.63226118042495 4.42418159710687e-69 miRanda -0.121557647488561 0.0168129413010743 NA NA NA hsa-miR-26a-5p RRM2 -0.631973911825762 2.12482912864596e-07 4.63226118042495 4.42418159710687e-69 miRNAWalker2_validate -0.815534106217093 5.77314966000411e-12 NA NA NA hsa-miR-28-5p RRM2 -0.466180213899448 0.00012482099258297 4.63226118042495 4.42418159710687e-69 miRanda -0.288329172529459 0.0185317842456254 NA NA NA hsa-miR-125a-5p RRM2B -0.347216996653838 0.0211699797450826 -0.157486703018183 0.31746185137698 miRanda -0.233425313520297 2.32498433528334e-06 NA NA NA hsa-miR-140-5p RRM2B -0.887888681168421 5.76545563709515e-08 -0.157486703018183 0.31746185137698 miRanda -0.19107088487685 2.0728757304335e-05 NA NA NA hsa-miR-186-5p RRM2B 1.01160002003511 1.0995153919986e-13 -0.157486703018183 0.31746185137698 mirMAP -0.211302027710062 7.85304171524234e-05 NA NA NA hsa-miR-26a-2-3p RRM2B 0.0966122083896723 0.587572958861438 -0.157486703018183 0.31746185137698 mirMAP -0.215467078213999 2.28312326232208e-07 NA NA NA hsa-miR-30c-5p RRM2B 0.724673207831053 1.32226261564805e-05 -0.157486703018183 0.31746185137698 mirMAP -0.151252870163962 0.000688912608252989 NA NA NA hsa-miR-30e-5p RRM2B 0.264119434405151 0.0488892309487705 -0.157486703018183 0.31746185137698 mirMAP -0.197438030022298 0.000431582308100917 NA NA NA hsa-miR-590-3p RRM2B 1.73212597984247 2.26504285146779e-20 -0.157486703018183 0.31746185137698 miRanda -0.107373232993264 0.00495601011567558 NA NA NA hsa-miR-7-1-3p RRM2B 1.83538862227934 3.8339472676544e-18 -0.157486703018183 0.31746185137698 mirMAP -0.10567790846392 0.00181008867980067 NA NA NA hsa-miR-2110 SERPINE1 1.57021301515342 7.33611160219404e-14 -0.506257443895086 0.074599930850929 miRNATAP -0.326924083018718 1.59036338528945e-07 NA NA NA hsa-miR-301a-3p SERPINE1 1.9416887079489 3.80655048022936e-21 -0.506257443895086 0.074599930850929 miRNAWalker2_validate;miRTarBase -0.193511329161878 0.00197180051645802 NA NA NA hsa-miR-30a-5p SERPINE1 0.204006364430255 0.420323839030139 -0.506257443895086 0.074599930850929 miRNATAP -0.124051886597879 0.0199832868091221 NA NA NA hsa-miR-30c-5p SERPINE1 0.724673207831053 1.32226261564805e-05 -0.506257443895086 0.074599930850929 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.320116960115344 6.79157788002547e-05 NA NA NA hsa-miR-30d-5p SERPINE1 0.577677794903206 0.000226725362226714 -0.506257443895086 0.074599930850929 miRNATAP -0.20895114016774 0.0153490720204196 NA NA NA hsa-miR-342-3p SERPINE1 0.909988426908683 3.51879055202261e-09 -0.506257443895086 0.074599930850929 PITA;miRanda -0.2052467357145 0.0178346495915523 NA NA NA hsa-miR-34c-5p SERPINE1 -0.27131143866706 0.619248345070017 -0.506257443895086 0.074599930850929 PITA;miRNATAP -0.148876384893019 8.21248442526567e-10 NA NA NA hsa-miR-378a-5p SERPINE1 0.548375041479493 0.0305578851647469 -0.506257443895086 0.074599930850929 MirTarget -0.262868976011592 5.60268128265104e-07 NA NA NA hsa-miR-425-5p SERPINE1 3.37583546071413 8.59535002332206e-48 -0.506257443895086 0.074599930850929 MirTarget;miRNATAP -0.110292527664149 0.0335136227968531 NA NA NA hsa-miR-769-5p SERPINE1 1.4141195738355 1.74458572237395e-16 -0.506257443895086 0.074599930850929 MirTarget;miRNATAP -0.342040378737015 5.91950698577066e-06 NA NA NA hsa-miR-942-5p SERPINE1 2.4506152128348 4.56477806519993e-27 -0.506257443895086 0.074599930850929 MirTarget -0.13218226532913 0.0195878336398297 NA NA NA hsa-miR-103a-2-5p SESN1 2.42870001778613 1.23381577013723e-24 -1.19019627083616 1.1816646801903e-17 MirTarget -0.180783483541262 2.17997570029904e-11 NA NA NA hsa-miR-146b-5p SESN1 1.31285313891779 3.27846083137877e-10 -1.19019627083616 1.1816646801903e-17 miRanda -0.22773679715732 3.88646348183728e-13 NA NA NA hsa-miR-15a-5p SESN1 1.77791080875961 1.15367449842886e-31 -1.19019627083616 1.1816646801903e-17 MirTarget;miRNATAP -0.145324993100439 0.000556001901568044 NA NA NA hsa-miR-15b-5p SESN1 2.49680902955696 1.79560488884714e-45 -1.19019627083616 1.1816646801903e-17 MirTarget;miRNATAP -0.248349628473109 3.40894759659201e-13 NA NA NA hsa-miR-16-5p SESN1 1.88120757008208 1.6207082610779e-32 -1.19019627083616 1.1816646801903e-17 MirTarget;miRNATAP -0.258013404175667 6.73590371333602e-11 NA NA NA hsa-miR-183-5p SESN1 6.01612233013103 1.47372794176386e-93 -1.19019627083616 1.1816646801903e-17 MirTarget -0.135010805470196 1.53555092877307e-13 NA NA NA hsa-miR-18a-3p SESN1 3.53840119922299 3.0702326386721e-42 -1.19019627083616 1.1816646801903e-17 MirTarget -0.223268914096921 3.34115286205989e-21 NA NA NA hsa-miR-200b-3p SESN1 4.56508753729609 3.8956399692993e-55 -1.19019627083616 1.1816646801903e-17 MirTarget;TargetScan -0.128304048642392 2.10543703644621e-10 NA NA NA hsa-miR-200c-3p SESN1 4.19740435353621 6.07734417092297e-49 -1.19019627083616 1.1816646801903e-17 MirTarget;miRNATAP -0.136445825356678 7.08251969495214e-11 24791940 We found that miR-200c overexpression increased cellular radiosensitivity by direct regulation of the oxidative stress response genes PRDX2 GAPB/Nrf2 and SESN1 in ways that inhibits DNA double-strand breaks repair increase levels of reactive oxygen species and upregulate p21 drug resistance lung cancer hsa-miR-21-5p SESN1 1.51489362035879 4.99155235011048e-20 -1.19019627083616 1.1816646801903e-17 miRNAWalker2_validate -0.258263783950898 3.65830420533539e-11 NA NA NA hsa-miR-24-3p SESN1 0.861226137512247 2.90830475095671e-09 -1.19019627083616 1.1816646801903e-17 miRNAWalker2_validate;MirTarget;miRNATAP -0.327115114869424 7.89962279961699e-13 NA NA NA hsa-miR-320b SESN1 1.09552613515995 1.0755423833529e-07 -1.19019627083616 1.1816646801903e-17 miRanda -0.124648276095686 0.000136730291243095 NA NA NA hsa-miR-429 SESN1 5.03984117897747 4.44936205318976e-55 -1.19019627083616 1.1816646801903e-17 MirTarget;PITA;miRanda;miRNATAP -0.124747035289472 7.32400703136821e-12 NA NA NA hsa-miR-501-3p SESN1 2.18792856444299 6.3664407099549e-25 -1.19019627083616 1.1816646801903e-17 MirTarget;miRNATAP -0.124586153385363 4.06650924890957e-05 NA NA NA hsa-miR-590-3p SESN1 1.73212597984247 2.26504285146779e-20 -1.19019627083616 1.1816646801903e-17 miRanda -0.19548571502468 1.67626946732877e-08 NA NA NA hsa-miR-590-5p SESN1 1.45684002317639 1.74263571329047e-15 -1.19019627083616 1.1816646801903e-17 miRanda -0.197618634009748 3.52424156600621e-08 NA NA NA hsa-miR-23b-3p SESN2 -0.940125237183254 5.29255710761003e-10 0.420641947281192 0.0036652256065414 miRNATAP -0.163184341730248 0.000264445487260297 NA NA NA hsa-miR-27b-3p SESN2 -0.936575637317446 2.43405225499419e-09 0.420641947281192 0.0036652256065414 miRNATAP -0.146061696296898 0.000724934304659731 NA NA NA hsa-miR-320a SESN2 0.502704491334741 0.00226252073794899 0.420641947281192 0.0036652256065414 mirMAP -0.118587524496263 0.00460930119319885 NA NA NA hsa-miR-335-5p SESN2 0.348427694169649 0.0962171361436908 0.420641947281192 0.0036652256065414 miRNAWalker2_validate -0.147033869564883 6.66947368495708e-06 NA NA NA hsa-miR-130a-3p SESN3 0.188483151884821 0.402581058443795 0.129022835968278 0.563342072440348 mirMAP -0.102759263587569 0.0291728754393218 NA NA NA hsa-miR-142-5p SESN3 1.99051987846668 1.30874707506504e-14 0.129022835968278 0.563342072440348 mirMAP -0.131964193829808 0.000845148347700907 NA NA NA hsa-miR-146b-5p SESN3 1.31285313891779 3.27846083137877e-10 0.129022835968278 0.563342072440348 PITA;miRanda;miRNATAP -0.145077258421183 0.00346885917148303 NA NA NA hsa-miR-16-2-3p SESN3 2.33811104285535 7.35637935757058e-33 0.129022835968278 0.563342072440348 mirMAP -0.102829495804113 0.0407737921800247 NA NA NA hsa-miR-192-3p SESN3 1.75017281711199 1.2581764844839e-09 0.129022835968278 0.563342072440348 MirTarget -0.100211925547823 0.00538344726357703 NA NA NA hsa-miR-30a-3p SESN3 0.307358101302791 0.229841318665456 0.129022835968278 0.563342072440348 mirMAP -0.207245141479587 3.99490053228926e-07 NA NA NA hsa-miR-32-5p SESN3 1.61585199704519 4.93530744182272e-20 0.129022835968278 0.563342072440348 MirTarget;miRNATAP -0.194835451253745 0.000704006659800592 NA NA NA hsa-miR-330-3p SESN3 1.76420587112262 1.73634190017122e-17 0.129022835968278 0.563342072440348 mirMAP -0.170184956503542 0.000532276209485329 NA NA NA hsa-miR-339-5p SESN3 1.79341790518551 3.04346641619717e-17 0.129022835968278 0.563342072440348 miRanda -0.100768146358612 0.0360572482149081 NA NA NA hsa-miR-454-3p SESN3 1.52878971973824 1.20025123958525e-19 0.129022835968278 0.563342072440348 mirMAP -0.140313473472409 0.0201665262633993 NA NA NA hsa-miR-511-5p SESN3 0.554715601076077 0.0348097472471271 0.129022835968278 0.563342072440348 MirTarget -0.105804074578759 0.00900461358699806 NA NA NA hsa-miR-550a-5p SESN3 1.68852119077479 6.89894923025492e-17 0.129022835968278 0.563342072440348 MirTarget -0.130897403564536 0.00969023557586965 NA NA NA hsa-miR-625-3p SESN3 0.992589228889688 5.90997361228204e-06 0.129022835968278 0.563342072440348 mirMAP -0.177749512940419 0.000190907039975714 NA NA NA hsa-miR-625-5p SESN3 1.10918025139677 1.4430215111181e-06 0.129022835968278 0.563342072440348 MirTarget -0.205931985388651 5.20529329772593e-06 NA NA NA hsa-miR-15b-5p SIAH1 2.49680902955696 1.79560488884714e-45 -0.625648858789827 1.92797621153852e-13 miRNATAP -0.115905620646415 2.52112005830345e-08 NA NA NA hsa-miR-16-5p SIAH1 1.88120757008208 1.6207082610779e-32 -0.625648858789827 1.92797621153852e-13 miRNATAP -0.147046522999528 7.21558103920146e-10 NA NA NA hsa-miR-505-5p SIAH1 0.91754482144444 1.3794595114153e-06 -0.625648858789827 1.92797621153852e-13 miRNAWalker2_validate -0.115455691155719 5.58865879824227e-08 NA NA NA hsa-miR-222-5p STEAP3 0.0863587509728097 0.719399526083386 0.906693839398955 0.000124181513262235 mirMAP -0.136549892779314 0.00381729742687999 NA NA NA hsa-let-7a-3p THBS1 1.03029456575871 2.05932484675687e-08 -2.38050486300434 8.75304846254371e-18 mirMAP -0.230023525084739 0.00173370436470145 NA NA NA hsa-let-7d-5p THBS1 0.769608372318022 2.02277615276328e-07 -2.38050486300434 8.75304846254371e-18 miRNAWalker2_validate -0.486307226890529 8.65403660804047e-08 NA NA NA hsa-let-7f-1-3p THBS1 0.993720810761227 6.0407149976796e-08 -2.38050486300434 8.75304846254371e-18 mirMAP -0.339895863814615 3.78605890431405e-06 NA NA NA hsa-miR-125a-3p THBS1 0.766248892008454 5.97048811423325e-05 -2.38050486300434 8.75304846254371e-18 miRanda -0.331604091618121 2.86467561148382e-06 NA NA NA hsa-miR-151a-3p THBS1 0.944646013243785 4.69249786473818e-12 -2.38050486300434 8.75304846254371e-18 MirTarget -0.695373743722873 5.13112874772156e-13 NA NA NA hsa-miR-155-5p THBS1 2.19935449503549 2.51453237821502e-16 -2.38050486300434 8.75304846254371e-18 miRNAWalker2_validate;mirMAP -0.16834115600407 0.000590361879560515 NA NA NA hsa-miR-16-1-3p THBS1 2.08698516922856 1.42419622445015e-24 -2.38050486300434 8.75304846254371e-18 MirTarget -0.391978824605635 3.43866072882179e-10 NA NA NA hsa-miR-17-5p THBS1 2.26819952852251 1.17080117367962e-29 -2.38050486300434 8.75304846254371e-18 miRNAWalker2_validate -0.515267261908826 6.03199334495254e-17 NA NA NA hsa-miR-181a-5p THBS1 0.965577301473587 3.6533705355927e-07 -2.38050486300434 8.75304846254371e-18 mirMAP -0.17661291151317 0.0133870150086893 NA NA NA hsa-miR-181b-5p THBS1 1.29743991759771 2.50613983144765e-11 -2.38050486300434 8.75304846254371e-18 mirMAP -0.247271202359774 0.000319237111052378 NA NA NA hsa-miR-181c-5p THBS1 0.59725303236225 0.0107673525407592 -2.38050486300434 8.75304846254371e-18 mirMAP -0.146029412607619 0.0123046942024461 NA NA NA hsa-miR-182-5p THBS1 5.17636717835373 1.04663621342014e-76 -2.38050486300434 8.75304846254371e-18 MirTarget -0.307073920282083 1.01777132863193e-14 24053448 Effects of anti miR 182 on TSP 1 expression in human colon cancer cells: there is a sense in antisense?; We investigate on the molecular mechanism by which miR-182 could regulate thrombospondin-1 TSP-1 expression a protein downregulated in CRC and inversely correlated with tumor vascularity and metastasis; miR-182 over-expressed in colorectal cancer CRC has like predictive target thrombospondin-1 TSP-1 a protein inversely correlated with tumor vascularity and metastasis that results downregulated in different types of cancer including CRC; We found that TSP-1 increased after transfection with anti-miR-182 and we showed that miR-182 targets TSP-1 3'UTR-mRNA in both cells; Our data suggest that miR-182 targets the anti-angiogenic factor TSP-1 and that anti-miR-182 determines an upregulation of TSP-1 expression in colon cancer cells metastasis colon cancer hsa-miR-186-5p THBS1 1.01160002003511 1.0995153919986e-13 -2.38050486300434 8.75304846254371e-18 mirMAP -0.543098259104612 2.41438685812501e-08 NA NA NA hsa-miR-18a-5p THBS1 3.22282140470889 1.9203457351048e-39 -2.38050486300434 8.75304846254371e-18 MirTarget -0.420656397837916 9.40347075371437e-18 NA NA NA hsa-miR-19a-3p THBS1 1.84830198357993 2.4710712042826e-20 -2.38050486300434 8.75304846254371e-18 MirTarget;mirMAP -0.456116988517492 9.29204123157682e-13 NA NA NA hsa-miR-19b-3p THBS1 1.34004947278979 5.26920324973452e-16 -2.38050486300434 8.75304846254371e-18 MirTarget;mirMAP -0.568250914723708 4.03690900701933e-13 NA NA NA hsa-miR-200a-5p THBS1 4.88923571940969 7.26134286540137e-61 -2.38050486300434 8.75304846254371e-18 MirTarget -0.342301442377175 3.52570472666346e-19 NA NA NA hsa-miR-200b-5p THBS1 4.83774625035774 1.7737491415346e-62 -2.38050486300434 8.75304846254371e-18 MirTarget -0.369837048995742 1.7059474231594e-21 NA NA NA hsa-miR-20a-5p THBS1 1.51276418035651 8.71307033346922e-13 -2.38050486300434 8.75304846254371e-18 miRNAWalker2_validate -0.440927291601209 7.38413211578831e-13 NA NA NA hsa-miR-218-5p THBS1 0.496805018594163 0.0671252638705632 -2.38050486300434 8.75304846254371e-18 mirMAP -0.180775100290437 0.000314360371598714 NA NA NA hsa-miR-3065-5p THBS1 2.75496955380748 2.37091362562179e-15 -2.38050486300434 8.75304846254371e-18 MirTarget -0.247399512922114 2.70319140187846e-10 NA NA NA hsa-miR-32-5p THBS1 1.61585199704519 4.93530744182272e-20 -2.38050486300434 8.75304846254371e-18 mirMAP -0.636318259172474 6.67052802602802e-19 NA NA NA hsa-miR-335-3p THBS1 1.32116009044055 1.0694366259168e-09 -2.38050486300434 8.75304846254371e-18 mirMAP -0.409161558657281 1.57485561142713e-11 NA NA NA hsa-miR-338-3p THBS1 0.429940853175601 0.097699268368127 -2.38050486300434 8.75304846254371e-18 MirTarget;PITA;miRanda -0.241955195705604 3.61303574765411e-06 NA NA NA hsa-miR-33a-3p THBS1 1.39444251212239 7.26586547385458e-13 -2.38050486300434 8.75304846254371e-18 MirTarget;mirMAP -0.336256957488003 8.65671542991954e-07 NA NA NA hsa-miR-421 THBS1 2.10188043858202 8.21949037851434e-21 -2.38050486300434 8.75304846254371e-18 miRanda -0.365625440908519 1.86114241856168e-10 NA NA NA hsa-miR-491-5p THBS1 1.22779907098202 1.78415304404366e-09 -2.38050486300434 8.75304846254371e-18 miRanda -0.302182793459541 4.03916300080789e-06 NA NA NA hsa-miR-500a-5p THBS1 0.803568840489829 3.89317331148068e-05 -2.38050486300434 8.75304846254371e-18 mirMAP -0.251751827074076 0.000303636009783692 NA NA NA hsa-miR-576-5p THBS1 1.30779208544019 1.20419097314618e-13 -2.38050486300434 8.75304846254371e-18 mirMAP -0.367646228645216 9.40046462136867e-07 NA NA NA hsa-miR-577 THBS1 3.23403680126545 4.85573112916202e-14 -2.38050486300434 8.75304846254371e-18 mirMAP -0.112544533960694 0.000330947333674446 NA NA NA hsa-miR-589-3p THBS1 1.69712322956823 3.70306979263175e-12 -2.38050486300434 8.75304846254371e-18 mirMAP -0.236328317481232 2.02301196348491e-05 NA NA NA hsa-miR-590-3p THBS1 1.73212597984247 2.26504285146779e-20 -2.38050486300434 8.75304846254371e-18 miRanda -0.411818877517185 2.44665855848244e-09 NA NA NA hsa-miR-590-5p THBS1 1.45684002317639 1.74263571329047e-15 -2.38050486300434 8.75304846254371e-18 miRanda;mirMAP -0.41228556595108 7.96685669960722e-09 NA NA NA hsa-miR-616-5p THBS1 1.97053352577988 6.50616168455898e-16 -2.38050486300434 8.75304846254371e-18 mirMAP -0.224738965116137 3.3332351151661e-05 NA NA NA hsa-miR-629-3p THBS1 2.65568346952191 8.96546275114201e-22 -2.38050486300434 8.75304846254371e-18 MirTarget -0.24169212985332 5.04391335222926e-07 NA NA NA hsa-miR-671-5p THBS1 2.3716249856662 4.71138196574897e-33 -2.38050486300434 8.75304846254371e-18 MirTarget -0.440019429888564 2.05958493438706e-12 NA NA NA hsa-miR-7-1-3p THBS1 1.83538862227934 3.8339472676544e-18 -2.38050486300434 8.75304846254371e-18 MirTarget;mirMAP -0.355090647754656 7.06513724296703e-09 NA NA NA hsa-miR-92a-3p THBS1 1.69110879028255 8.04580501722806e-26 -2.38050486300434 8.75304846254371e-18 miRNAWalker2_validate -0.749083511211979 1.60440139804765e-22 NA NA NA hsa-miR-940 THBS1 3.48873734617979 1.86622896910559e-36 -2.38050486300434 8.75304846254371e-18 MirTarget -0.366835107644412 5.6840572326631e-16 NA NA NA hsa-miR-98-5p THBS1 1.05654915824416 2.25069999789746e-15 -2.38050486300434 8.75304846254371e-18 miRNAWalker2_validate -0.707712834065435 4.96075567676847e-13 NA NA NA hsa-miR-26a-5p TNFRSF10B -0.631973911825762 2.12482912864596e-07 0.822199658997421 8.1142816616948e-08 mirMAP -0.275543424183851 4.62817286518087e-06 NA NA NA hsa-miR-28-5p TNFRSF10B -0.466180213899448 0.00012482099258297 0.822199658997421 8.1142816616948e-08 miRanda -0.124496389514824 0.0423064577948475 NA NA NA hsa-miR-542-3p TNFRSF10B -2.02613612825205 8.22372377712865e-20 0.822199658997421 8.1142816616948e-08 miRanda -0.103564310329005 0.00106316058467598 NA NA NA hsa-miR-30d-5p TP53 0.577677794903206 0.000226725362226714 0.498837102942024 0.00520255799128458 miRNAWalker2_validate -0.172511137240746 0.00144218092065489 NA NA NA hsa-miR-10b-5p TP73 -1.71808089912719 3.75291415380712e-14 2.12978903016336 3.76510395910864e-08 mirMAP -0.178248056334422 0.0256745290047586 NA NA NA hsa-miR-296-5p TP73 -0.208779469897537 0.549226448418253 2.12978903016336 3.76510395910864e-08 mirMAP -0.171993346628558 0.00159859603152295 NA NA NA hsa-miR-31-5p TP73 1.8950492867952 0.000263457461731176 2.12978903016336 3.76510395910864e-08 mirMAP -0.11848692704457 0.00138978096501232 NA NA NA hsa-miR-542-3p TP73 -2.02613612825205 8.22372377712865e-20 2.12978903016336 3.76510395910864e-08 mirMAP -0.173853008046563 0.0306407438899921 NA NA NA hsa-miR-101-3p ZMAT3 -1.48434639686706 2.22407930927359e-19 -0.351575278224335 0.0928788528375247 MirTarget -0.136253606537057 0.0187965509083316 NA NA NA hsa-miR-106a-5p ZMAT3 2.93836356285531 1.22956096158575e-21 -0.351575278224335 0.0928788528375247 mirMAP -0.109068143732094 0.000358135133889014 NA NA NA hsa-miR-106b-5p ZMAT3 2.17788294729604 3.35797658884941e-37 -0.351575278224335 0.0928788528375247 mirMAP -0.316642545890424 1.71452185098841e-09 NA NA NA hsa-miR-130b-3p ZMAT3 3.42039109436067 3.89459455628164e-49 -0.351575278224335 0.0928788528375247 MirTarget -0.120974320288943 0.00145578728473487 NA NA NA hsa-miR-148b-5p ZMAT3 2.24566117508049 6.39491669468652e-28 -0.351575278224335 0.0928788528375247 MirTarget -0.138699712693095 0.00229399037232546 NA NA NA hsa-miR-15a-5p ZMAT3 1.77791080875961 1.15367449842886e-31 -0.351575278224335 0.0928788528375247 MirTarget -0.192482444267517 0.00161152756310946 NA NA NA hsa-miR-15b-3p ZMAT3 3.24791797249066 5.10037250255355e-48 -0.351575278224335 0.0928788528375247 mirMAP -0.124855842217238 0.00166572218958804 NA NA NA hsa-miR-15b-5p ZMAT3 2.49680902955696 1.79560488884714e-45 -0.351575278224335 0.0928788528375247 MirTarget -0.170667749434932 0.000741852873269643 NA NA NA hsa-miR-17-5p ZMAT3 2.26819952852251 1.17080117367962e-29 -0.351575278224335 0.0928788528375247 mirMAP -0.25399982739052 2.64468706913105e-08 NA NA NA hsa-miR-18a-3p ZMAT3 3.53840119922299 3.0702326386721e-42 -0.351575278224335 0.0928788528375247 MirTarget -0.133780769178587 0.000176759452091104 NA NA NA hsa-miR-193a-3p ZMAT3 0.422281760608533 0.0531653219715062 -0.351575278224335 0.0928788528375247 MirTarget -0.170655786622789 0.0001667856031558 NA NA NA hsa-miR-19a-3p ZMAT3 1.84830198357993 2.4710712042826e-20 -0.351575278224335 0.0928788528375247 MirTarget -0.273523752115424 4.9301549846219e-09 NA NA NA hsa-miR-19b-3p ZMAT3 1.34004947278979 5.26920324973452e-16 -0.351575278224335 0.0928788528375247 MirTarget -0.24304511197435 2.72577141514337e-05 NA NA NA hsa-miR-20a-5p ZMAT3 1.51276418035651 8.71307033346922e-13 -0.351575278224335 0.0928788528375247 mirMAP -0.319590435181867 7.61385775812259e-13 NA NA NA hsa-miR-27a-3p ZMAT3 1.20835958303386 1.23147423005989e-12 -0.351575278224335 0.0928788528375247 MirTarget;miRNATAP -0.162069880654372 0.00449718081461404 NA NA NA hsa-miR-301a-3p ZMAT3 1.9416887079489 3.80655048022936e-21 -0.351575278224335 0.0928788528375247 MirTarget -0.177262328781875 0.000112534652071083 NA NA NA hsa-miR-320c ZMAT3 0.733362580458909 0.00057735467707277 -0.351575278224335 0.0928788528375247 miRanda;mirMAP -0.122572127955623 0.00988702215670918 NA NA NA hsa-miR-361-5p ZMAT3 0.505614361096266 0.000106749108296893 -0.351575278224335 0.0928788528375247 MirTarget;miRNATAP -0.35525635699669 2.85236329126855e-06 NA NA NA hsa-miR-362-3p ZMAT3 0.538723455753891 0.00242035379543243 -0.351575278224335 0.0928788528375247 MirTarget -0.128610612624736 0.0219027431782063 NA NA NA hsa-miR-374a-5p ZMAT3 -0.0832626833277432 0.509637142371946 -0.351575278224335 0.0928788528375247 mirMAP -0.187411629509048 0.0190505332714891 NA NA NA hsa-miR-421 ZMAT3 2.10188043858202 8.21949037851434e-21 -0.351575278224335 0.0928788528375247 mirMAP;miRNATAP -0.198324734694846 2.38843962412659e-06 NA NA NA hsa-miR-454-3p ZMAT3 1.52878971973824 1.20025123958525e-19 -0.351575278224335 0.0928788528375247 MirTarget -0.114610576363875 0.0432749682706092 NA NA NA hsa-miR-501-5p ZMAT3 1.63365089104021 2.93689344466334e-15 -0.351575278224335 0.0928788528375247 MirTarget;mirMAP -0.215950421185729 2.83882410866866e-06 NA NA NA hsa-miR-660-5p ZMAT3 0.20661370223084 0.267201322788119 -0.351575278224335 0.0928788528375247 mirMAP -0.246536618936574 3.42104402252299e-06 NA NA NA hsa-miR-671-5p ZMAT3 2.3716249856662 4.71138196574897e-33 -0.351575278224335 0.0928788528375247 MirTarget -0.102202227425083 0.0280969663897782 NA NA NA hsa-miR-93-5p ZMAT3 2.57501728456111 3.73950852324834e-39 -0.351575278224335 0.0928788528375247 mirMAP -0.277457911012472 9.24460143844722e-10 NA NA NA