miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-21-5p ABAT 1.51084295924322 1.54551622173259e-34 -2.16281544019696 3.93403666966165e-18 MirTarget -0.92627033209199 4.24502265699131e-25 NA NA NA hsa-miR-21-5p ABCA1 1.51084295924322 1.54551622173259e-34 -1.09636606966708 7.96780532063531e-16 mirMAP -0.285550113313049 1.80466337738302e-08 NA NA NA hsa-miR-21-5p ABCA10 1.51084295924322 1.54551622173259e-34 -1.06365527432395 3.8402730713037e-05 mirMAP -0.450522220378371 1.73922913330204e-06 NA NA NA hsa-miR-21-5p ABCD3 1.51084295924322 1.54551622173259e-34 -0.902074727071182 3.19852861835435e-18 miRNAWalker2_validate -0.37544948577207 7.02368348393481e-24 NA NA NA hsa-miR-21-3p ACAD11 -0.484219378716006 0.00299941098417659 -1.86007980131262 1.45750872387156e-27 MirTarget -0.241642308857888 8.02354819436745e-06 NA NA NA hsa-miR-21-5p ACAT1 1.51084295924322 1.54551622173259e-34 -1.61982651854581 1.24371695502075e-23 miRNAWalker2_validate -0.618343622608645 6.65323276600699e-26 NA NA NA hsa-miR-21-5p ACBD5 1.51084295924322 1.54551622173259e-34 -0.747355613421877 3.97123554106721e-11 miRNAWalker2_validate;miRNATAP -0.327371319483953 1.04628864477185e-15 NA NA NA hsa-miR-21-5p ACVR2A 1.51084295924322 1.54551622173259e-34 -0.549017310001321 8.35009567429434e-13 miRNATAP -0.169607048854855 1.73814737029117e-09 NA NA NA hsa-miR-21-5p AIM1 1.51084295924322 1.54551622173259e-34 -1.46469358428655 1.2646301993896e-12 miRNAWalker2_validate -0.351580360018553 4.71693671375075e-06 NA NA NA hsa-miR-21-5p AKAP6 1.51084295924322 1.54551622173259e-34 -0.968677768825482 1.23169099553274e-07 MirTarget -0.541064541522812 1.32687890881404e-16 NA NA NA hsa-miR-21-5p AKAP9 1.51084295924322 1.54551622173259e-34 -0.793970431680178 2.34289104389822e-08 miRNAWalker2_validate -0.290501060527038 2.26170828191353e-08 NA NA NA hsa-miR-21-5p AKIRIN1 1.51084295924322 1.54551622173259e-34 -0.726962157087393 2.07022326698311e-14 miRNATAP -0.126515681462167 0.000383737994199711 NA NA NA hsa-miR-21-5p AKT2 1.51084295924322 1.54551622173259e-34 -0.343608685894869 1.25471482871759e-05 miRNAWalker2_validate -0.246625533117166 4.43585626011661e-19 NA NA NA hsa-miR-221-3p AMOT 1.12244756358612 3.48370308238012e-13 -0.89778365109471 0.000787406411317435 miRNAWalker2_validate -0.321933225160784 9.090255444677e-05 NA NA NA hsa-miR-21-5p ANKRD28 1.51084295924322 1.54551622173259e-34 -0.356990023333576 5.78744492945674e-06 miRNAWalker2_validate -0.174630027645497 7.45048491311049e-10 NA NA NA hsa-miR-21-5p ANKRD46 1.51084295924322 1.54551622173259e-34 -0.781694714145421 7.42587619630524e-10 miRNAWalker2_validate;miRTarBase -0.406156253153047 2.75148411405391e-19 21219636 Knockdown of miR-21 significantly increased the expression of ANKRD46 at both mRNA and protein levels; ANKRD46 is newly identified as a direct target of miR-21 in BC breast cancer hsa-miR-21-5p ANO3 1.51084295924322 1.54551622173259e-34 -2.29674689433524 3.8766367804959e-09 mirMAP -0.983667262898009 3.76138226056672e-12 NA NA NA hsa-miR-21-5p AP1AR 1.51084295924322 1.54551622173259e-34 -0.606234109288887 7.43586488835338e-08 MirTarget;miRNATAP -0.254779994139421 4.6831692514021e-10 NA NA NA hsa-miR-21-5p APC 1.51084295924322 1.54551622173259e-34 -0.178106776451097 0.0679159506996304 miRNAWalker2_validate -0.170963654395431 1.15664392926358e-06 23773491;24832083 The prognostic significance of APC gene mutation and miR 21 expression in advanced stage colorectal cancer; The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients; APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction; APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation; In Taiwan downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation; APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC especially in patients with advanced disease;MicroRNA 21 promotes tumour malignancy via increased nuclear translocation of β catenin and predicts poor outcome in APC mutated but not in APC wild type colorectal cancer; However in our preliminary data the prognostic value of miR-21 levels was observed only in adenomatous polyposis coli APC-mutated tumours not in APC-wild-type tumours; We enrolled 165 colorectal tumour to determine APC mutation miR-21 levels and nuclear β-catenin expression by direct sequencing real-time PCR and immunohistochemistry staging; colorectal cancer;colorectal cancer hsa-miR-21-5p APOLD1 1.51084295924322 1.54551622173259e-34 0.453740241904891 0.00928610512501684 miRNAWalker2_validate -0.1771891442795 0.0054502772943704 NA NA NA hsa-miR-221-3p AQP3 1.12244756358612 3.48370308238012e-13 -1.90428727360161 1.63416767229399e-21 MirTarget -0.171766599201336 0.00794925207301195 NA NA NA hsa-miR-221-3p ARAP2 1.12244756358612 3.48370308238012e-13 -1.13466869294013 1.21001797842144e-11 MirTarget -0.139559068683898 0.00818110075154483 NA NA NA hsa-miR-21-5p ARHGAP21 1.51084295924322 1.54551622173259e-34 -0.0901325619855351 0.372735683508637 miRNAWalker2_validate -0.109477885593189 0.00286441130824444 NA NA NA hsa-miR-21-5p ARHGAP24 1.51084295924322 1.54551622173259e-34 -0.989759863701917 1.90104401611984e-08 MirTarget;miRNATAP -0.361321405537448 2.02161621269502e-08 NA NA NA hsa-miR-21-5p ARHGAP32 1.51084295924322 1.54551622173259e-34 -0.199023023372693 0.099211320915758 MirTarget -0.291141695246814 1.24484805534917e-11 NA NA NA hsa-miR-221-3p ARHGAP42 1.12244756358612 3.48370308238012e-13 -1.24308952548647 4.86146691234769e-14 miRNAWalker2_validate;MirTarget;miRNATAP -0.402522335066655 1.85507356799353e-15 NA NA NA hsa-miR-21-5p ARHGEF12 1.51084295924322 1.54551622173259e-34 -0.860756828496805 1.466966277395e-15 miRNAWalker2_validate;MirTarget -0.259576540298464 7.48464022130423e-11 NA NA NA hsa-miR-21-5p ARID4A 1.51084295924322 1.54551622173259e-34 -0.895975441173601 4.13512367982488e-14 miRNAWalker2_validate -0.430336670497644 1.70726642436494e-24 NA NA NA hsa-miR-21-3p ARMC5 -0.484219378716006 0.00299941098417659 -0.651415856794464 1.25785383667628e-05 mirMAP -0.114056843498903 0.0110888666303187 NA NA NA hsa-miR-21-5p ART4 1.51084295924322 1.54551622173259e-34 -1.74425490375507 1.67364109265157e-09 mirMAP -0.483938253913385 6.01331559838995e-06 NA NA NA hsa-miR-21-3p ASB4 -0.484219378716006 0.00299941098417659 -0.619984466341846 0.0668344671524796 MirTarget -0.460026681655635 4.36140868711869e-06 NA NA NA hsa-miR-221-3p ASB4 1.12244756358612 3.48370308238012e-13 -0.619984466341846 0.0668344671524796 MirTarget -0.338696773167931 0.00111155215175412 NA NA NA hsa-miR-221-3p ASB7 1.12244756358612 3.48370308238012e-13 -0.199738778844576 0.00927477847644337 miRNATAP -0.101705955765497 1.34211428398082e-05 NA NA NA hsa-miR-221-3p ASPA 1.12244756358612 3.48370308238012e-13 -2.87708424623409 2.78732403857851e-24 MirTarget -0.664434785455406 1.31990121226134e-13 NA NA NA hsa-miR-21-5p ASPN 1.51084295924322 1.54551622173259e-34 -1.64007252962853 7.34792651154405e-10 MirTarget;miRNATAP -0.195771684549619 0.0490195828246832 NA NA NA hsa-miR-221-3p ASXL3 1.12244756358612 3.48370308238012e-13 -2.68929167531641 1.94609933734597e-13 miRNAWalker2_validate -0.946333249427835 2.29633026363543e-17 NA NA NA hsa-miR-21-5p ATAD2B 1.51084295924322 1.54551622173259e-34 -0.0635507129186372 0.479097593847395 miRNAWalker2_validate -0.136102764343005 2.65124149284912e-05 NA NA NA hsa-miR-21-5p ATF2 1.51084295924322 1.54551622173259e-34 -0.239353619837203 0.156190712870845 miRNAWalker2_validate -0.180342957288245 0.00335655702560994 NA NA NA hsa-miR-221-3p ATL2 1.12244756358612 3.48370308238012e-13 -0.0961038265935708 0.239507878733616 miRNAWalker2_validate -0.101275516664927 4.55377195161898e-05 NA NA NA hsa-miR-21-5p ATMIN 1.51084295924322 1.54551622173259e-34 -0.371002750700502 5.51678829659403e-08 miRNAWalker2_validate -0.12457794175966 5.46986481967043e-07 NA NA NA hsa-miR-21-5p ATP11B 1.51084295924322 1.54551622173259e-34 -0.346367156166827 0.00133271118765081 miRNAWalker2_validate -0.160915560238489 4.13017098875205e-05 NA NA NA hsa-miR-21-5p ATP2B2 1.51084295924322 1.54551622173259e-34 -0.395324820466063 0.155178018219404 mirMAP -0.239511793571401 0.0183742080487048 NA NA NA hsa-miR-21-5p ATRN 1.51084295924322 1.54551622173259e-34 0.11840720862332 0.341728688804806 miRNATAP -0.171300205806303 0.000147158588937355 NA NA NA hsa-miR-21-5p ATRNL1 1.51084295924322 1.54551622173259e-34 -2.27538406759707 4.17399658519262e-07 MirTarget -0.654556532527154 7.71322402871404e-05 NA NA NA hsa-miR-21-5p ATRX 1.51084295924322 1.54551622173259e-34 -0.0677373088337614 0.487587480385071 miRNAWalker2_validate -0.103911084514062 0.00334972263417209 NA NA NA hsa-miR-21-5p ATXN3 1.51084295924322 1.54551622173259e-34 -0.293269047017985 0.000369461897713509 mirMAP -0.22240426764001 2.59427646320707e-14 NA NA NA hsa-miR-21-5p AUTS2 1.51084295924322 1.54551622173259e-34 -1.19765271507382 9.8904416911024e-07 miRNAWalker2_validate -0.852360135392829 2.65946752125067e-23 NA NA NA hsa-miR-21-3p B4GALT6 -0.484219378716006 0.00299941098417659 0.170595319626529 0.279200848517993 MirTarget -0.105486608622757 0.0248281640834649 NA NA NA hsa-miR-21-3p BACE1 -0.484219378716006 0.00299941098417659 -0.448698228025254 5.54093323862846e-07 mirMAP -0.163277479909995 7.18922032555605e-10 NA NA NA hsa-miR-21-5p BACE1 1.51084295924322 1.54551622173259e-34 -0.448698228025254 5.54093323862846e-07 mirMAP -0.285520961360197 1.62841748241726e-19 NA NA NA hsa-miR-21-5p BCL6 1.51084295924322 1.54551622173259e-34 -0.381728718096561 0.00304721869179574 miRNAWalker2_validate -0.188140069576131 5.95723698698918e-05 NA NA NA hsa-miR-21-5p BDH2 1.51084295924322 1.54551622173259e-34 -1.59270294219311 1.02319078238481e-23 miRNAWalker2_validate -0.56515861454063 2.8021537723332e-22 NA NA NA hsa-miR-21-5p BMP2K 1.51084295924322 1.54551622173259e-34 -0.818475794333253 1.85957627715201e-10 mirMAP -0.123948593677391 0.00963381579892384 NA NA NA hsa-miR-21-5p BMPR2 1.51084295924322 1.54551622173259e-34 -0.735692104466334 2.00485450351153e-12 miRNAWalker2_validate;miRTarBase;MirTarget -0.284574090091544 7.19521500454622e-14 NA NA NA hsa-miR-221-3p BNIP3 1.12244756358612 3.48370308238012e-13 -0.56708142845002 7.502879446291e-06 miRNAWalker2_validate -0.191683138524634 8.17069300196437e-07 NA NA NA hsa-miR-221-3p BNIP3L 1.12244756358612 3.48370308238012e-13 -0.67007493110919 3.59838811073751e-10 miRNAWalker2_validate;miRTarBase -0.123031734398075 0.000226666656146561 NA NA NA hsa-miR-21-5p BRD1 1.51084295924322 1.54551622173259e-34 -0.222201860531052 0.011729440795329 MirTarget -0.100100275047052 0.00179805204947613 NA NA NA hsa-miR-221-3p BRWD1 1.12244756358612 3.48370308238012e-13 -0.472319203635839 2.18876455365102e-07 MirTarget -0.143440565347809 3.0138189803526e-07 NA NA NA hsa-miR-21-5p C16orf52 1.51084295924322 1.54551622173259e-34 -0.498924156279034 2.0232126667381e-06 miRNATAP -0.288699972211743 1.38137136690354e-14 NA NA NA hsa-miR-21-5p C1orf226 1.51084295924322 1.54551622173259e-34 0.294177134343668 0.072174506797414 mirMAP -0.125474880304265 0.0358426145551995 NA NA NA hsa-miR-21-3p C22orf29 -0.484219378716006 0.00299941098417659 1.09019898263552 1.10984149710713e-27 mirMAP -0.11290483781846 0.000373147888781202 NA NA NA hsa-miR-21-5p CADM2 1.51084295924322 1.54551622173259e-34 -2.31674580160095 5.89007396472084e-10 mirMAP;miRNATAP -0.379737941440532 0.00647561026230968 NA NA NA hsa-miR-21-5p CALD1 1.51084295924322 1.54551622173259e-34 -0.530621733887527 1.64156095327975e-08 miRNAWalker2_validate;MirTarget -0.273877053450713 3.45477925002901e-16 NA NA NA hsa-miR-21-3p CAMK2A -0.484219378716006 0.00299941098417659 -0.327454379942138 0.356907439672438 MirTarget -0.428695676613169 4.7255606363298e-05 NA NA NA hsa-miR-21-3p CAMLG -0.484219378716006 0.00299941098417659 0.418245964487841 9.85419489975729e-07 MirTarget -0.103676946396764 4.991884602039e-05 NA NA NA hsa-miR-221-3p CAMTA1 1.12244756358612 3.48370308238012e-13 -0.308821305875669 0.001102834823798 MirTarget -0.128246997831631 9.23948054031477e-06 NA NA NA hsa-miR-21-5p CASC4 1.51084295924322 1.54551622173259e-34 -0.459665382720857 2.26052627896481e-06 miRNATAP -0.140945392919455 7.51563165261554e-05 NA NA NA hsa-miR-221-3p CASR 1.12244756358612 3.48370308238012e-13 -3.09354922799373 1.45515579446105e-14 MirTarget -0.387673661676025 0.00241027141784746 NA NA NA hsa-miR-221-3p CBWD3 1.12244756358612 3.48370308238012e-13 -0.675462571944565 1.19900987247376e-06 MirTarget -0.164847279062406 0.000129011336258364 NA NA NA hsa-miR-21-3p CCDC117 -0.484219378716006 0.00299941098417659 0.129046545647341 0.171605248088927 MirTarget -0.185148962247655 1.43800107534829e-11 NA NA NA hsa-miR-21-5p CCDC121 1.51084295924322 1.54551622173259e-34 0.0857156966036231 0.430175996609146 MirTarget -0.211628505009972 5.68139329018811e-08 NA NA NA hsa-miR-21-5p CCDC152 1.51084295924322 1.54551622173259e-34 -1.09230945352764 5.84433015164347e-09 mirMAP -0.609464701764088 6.12032620010967e-20 NA NA NA hsa-miR-21-3p CCDC158 -0.484219378716006 0.00299941098417659 -1.41478029977726 5.24188374029468e-08 MirTarget -0.190226957780397 0.0158615284716201 NA NA NA hsa-miR-21-5p CCNG1 1.51084295924322 1.54551622173259e-34 -0.0461189096804278 0.640330790441284 miRNAWalker2_validate -0.131168170626415 0.000240629548489461 NA NA NA hsa-miR-221-3p CD4 1.12244756358612 3.48370308238012e-13 -2.19805487067597 2.60882227268234e-32 MirTarget;miRNATAP -0.346525489965451 1.27856306501111e-08 NA NA NA hsa-miR-21-3p CDADC1 -0.484219378716006 0.00299941098417659 -0.680352220690451 3.27861198316647e-09 MirTarget -0.216582777263197 2.45453470573157e-10 NA NA NA hsa-miR-21-3p CDC14B -0.484219378716006 0.00299941098417659 -1.18862488716786 6.19212759828214e-11 mirMAP -0.297956469233426 5.31433385530136e-08 NA NA NA hsa-miR-21-3p CDK14 -0.484219378716006 0.00299941098417659 -0.0249968813971337 0.858766472747201 MirTarget -0.146413804998751 0.000440358018181749 NA NA NA hsa-miR-221-3p CDKN1B 1.12244756358612 3.48370308238012e-13 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.153438831523776 5.56071406720925e-10 20146005;23637992;19953484;23939688;19126397;23967190;17569667;22992757;17721077;20461750 Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression staging;tumorigenesis;progression;;;;;;;;poor survival liver cancer;breast cancer;glioblastoma;breast cancer;melanoma;pancreatic cancer;prostate cancer;breast cancer;glioblastoma;ovarian cancer hsa-miR-21-3p CELF1 -0.484219378716006 0.00299941098417659 -0.38329898141669 2.26246188708636e-05 MirTarget -0.108499079497582 5.78878390245103e-05 NA NA NA hsa-miR-21-5p CLCN5 1.51084295924322 1.54551622173259e-34 -0.778462528596492 1.09010470162582e-08 miRNAWalker2_validate;mirMAP -0.326165630569137 4.23073780387319e-11 NA NA NA hsa-miR-221-3p CLN8 1.12244756358612 3.48370308238012e-13 -0.897397341277566 2.6962794902646e-12 mirMAP -0.139109615720654 0.000570745837446507 NA NA NA hsa-miR-21-5p CLOCK 1.51084295924322 1.54551622173259e-34 -0.52744295488734 0.00745405676073919 miRNAWalker2_validate -0.260389633076203 0.000290149185096459 NA NA NA hsa-miR-21-3p CLYBL -0.484219378716006 0.00299941098417659 -1.25097168648654 5.78163364095158e-13 mirMAP -0.139496521815271 0.00886851711821237 NA NA NA hsa-miR-221-3p CNRIP1 1.12244756358612 3.48370308238012e-13 -0.549923028422068 3.33463829283631e-05 miRNAWalker2_validate -0.120503414878902 0.00329937879148511 NA NA NA hsa-miR-21-5p CNTFR 1.51084295924322 1.54551622173259e-34 -3.51076939120689 5.09275920906821e-11 miRNATAP -1.10448148913803 2.07872949243695e-08 NA NA NA hsa-miR-21-3p COBL -0.484219378716006 0.00299941098417659 -0.625889423404631 0.000231830653158842 MirTarget -0.142502531304083 0.00519789860115497 NA NA NA hsa-miR-21-3p COBLL1 -0.484219378716006 0.00299941098417659 -1.47196788797961 8.44363885667744e-17 mirMAP -0.388079457932599 3.536745325947e-13 NA NA NA hsa-miR-21-5p COBLL1 1.51084295924322 1.54551622173259e-34 -1.47196788797961 8.44363885667744e-17 miRNAWalker2_validate -0.802903087494741 1.14645324287371e-39 NA NA NA hsa-miR-21-5p CPEB3 1.51084295924322 1.54551622173259e-34 -2.73760799365771 6.08365930201296e-43 miRNAWalker2_validate;MirTarget;miRNATAP -0.980746493840046 7.30211745183844e-41 NA NA NA hsa-miR-221-3p CPNE8 1.12244756358612 3.48370308238012e-13 -0.764323108203908 3.17718650291404e-08 MirTarget;miRNATAP -0.299051871575289 1.13961265873534e-12 NA NA NA hsa-miR-21-5p CREBL2 1.51084295924322 1.54551622173259e-34 -0.86271516459951 5.41073501751202e-18 miRNATAP -0.279086534335069 3.23834303999446e-14 NA NA NA hsa-miR-221-3p CREBL2 1.12244756358612 3.48370308238012e-13 -0.86271516459951 5.41073501751202e-18 MirTarget -0.263088230630059 1.03665184994387e-17 NA NA NA hsa-miR-21-5p CRIM1 1.51084295924322 1.54551622173259e-34 -0.427323666964741 0.0396278582056627 miRNATAP -0.151258114496889 0.0465522385435014 NA NA NA hsa-miR-21-3p CRLS1 -0.484219378716006 0.00299941098417659 -0.215826506189612 0.0806992664086582 MirTarget -0.269496629342567 4.64769822014688e-14 NA NA NA hsa-miR-21-5p CSNK1A1 1.51084295924322 1.54551622173259e-34 -0.337358696279789 1.0388780822195e-09 miRNAWalker2_validate -0.104077813571288 2.3200921239842e-07 NA NA NA hsa-miR-21-3p CTNNA3 -0.484219378716006 0.00299941098417659 -2.93718508917758 1.13925248568176e-18 MirTarget -0.407566699322434 8.0112613537227e-05 NA NA NA hsa-miR-221-3p CXCL12 1.12244756358612 3.48370308238012e-13 -3.59146357807385 1.03718574480494e-35 MirTarget -0.313249809796118 0.00117662736734837 NA NA NA hsa-miR-21-5p CYBRD1 1.51084295924322 1.54551622173259e-34 -1.29333537908526 9.44725361000613e-10 miRNAWalker2_validate -0.314388439169356 5.96588466109424e-05 NA NA NA hsa-miR-221-3p CYP1B1 1.12244756358612 3.48370308238012e-13 -0.0782876655143347 0.760967318096207 miRNAWalker2_validate;MirTarget -0.243561056612926 0.002022863297573 NA NA NA hsa-miR-21-3p CYP4F3 -0.484219378716006 0.00299941098417659 -1.55307662086169 1.07679103282901e-07 mirMAP -0.285264053607143 0.00124770208201665 NA NA NA hsa-miR-21-5p CYP4V2 1.51084295924322 1.54551622173259e-34 -1.71426542413107 2.57937029230203e-24 miRNAWalker2_validate -0.70899133339694 8.38005084001024e-32 NA NA NA hsa-miR-21-5p DAAM1 1.51084295924322 1.54551622173259e-34 -0.820319075229238 3.16122030911227e-05 miRNAWalker2_validate -0.578036099340168 1.45141919650478e-16 NA NA NA hsa-miR-21-5p DCAF10 1.51084295924322 1.54551622173259e-34 -0.27023416404054 0.000162269494983322 miRNAWalker2_validate -0.1886444046417 1.1767746170605e-13 NA NA NA hsa-miR-21-5p DCAF8 1.51084295924322 1.54551622173259e-34 -0.0265942497922618 0.751662897530488 miRNAWalker2_validate -0.162351371603683 6.7996925496588e-08 NA NA NA hsa-miR-221-3p DCUN1D1 1.12244756358612 3.48370308238012e-13 -0.454096561519516 2.98980089676835e-05 MirTarget -0.182188557878299 4.09071502586311e-08 NA NA NA hsa-miR-21-3p DCUN1D4 -0.484219378716006 0.00299941098417659 -0.241051443609763 0.00351891274628995 MirTarget -0.124696780466938 2.96800741020015e-07 NA NA NA hsa-miR-221-3p DCUN1D4 1.12244756358612 3.48370308238012e-13 -0.241051443609763 0.00351891274628995 MirTarget -0.100305199054875 7.0338674223595e-05 NA NA NA hsa-miR-21-5p DDAH1 1.51084295924322 1.54551622173259e-34 -0.918386843412848 3.01044229943748e-09 miRNAWalker2_validate -0.392903247297597 2.61310628460181e-12 26741162 In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX lung cancer hsa-miR-221-3p DDAH1 1.12244756358612 3.48370308238012e-13 -0.918386843412848 3.01044229943748e-09 miRNAWalker2_validate -0.231632930713386 1.41877548281945e-06 NA NA NA hsa-miR-21-3p DDI2 -0.484219378716006 0.00299941098417659 -1.53096229477393 2.81259482387215e-06 mirMAP -0.271144570472602 0.00597740800126801 NA NA NA hsa-miR-21-5p DDR2 1.51084295924322 1.54551622173259e-34 -0.840962754160993 0.00127709673572228 miRNAWalker2_validate -0.303610798872828 0.00148820739751815 NA NA NA hsa-miR-21-5p DDX3X 1.51084295924322 1.54551622173259e-34 -0.695107825481528 1.74554774697009e-17 miRNAWalker2_validate -0.201463309127107 2.71491429159216e-11 NA NA NA hsa-miR-221-3p DDX3X 1.12244756358612 3.48370308238012e-13 -0.695107825481528 1.74554774697009e-17 miRNATAP -0.100622298174859 0.000104064592416466 NA NA NA hsa-miR-221-3p DGKE 1.12244756358612 3.48370308238012e-13 -0.855065779351597 4.0361991167554e-06 miRNATAP -0.357373219511501 2.59209263594667e-10 NA NA NA hsa-miR-221-3p DIRAS3 1.12244756358612 3.48370308238012e-13 -4.17016298624992 2.73003492088956e-33 miRTarBase -0.985400240957357 7.11911280907473e-19 21071579;23801152;22117988 Further studies on a new mechanism of ARHI downregulation showed a significant inverse relationship between ARHI and miR-221 and 222 which were upregulated in prostate cancer cell lines; Transfection of miR-221 and 222 inhibitors into PC-3 cells caused a significant induction of ARHI expression; A direct interaction of miR-221 or 222 with a target site on the 3'UTR of ARHI was confirmed by a dual luciferase pMIR-REPORT assay; Finally we also found that genistein upregulates ARHI by downregulating miR-221 and 222 in PC-3 cells;Effects of ARHI on breast cancer cell biological behavior regulated by microRNA 221; The downregulation of ARHI was regulated by miR-221 in prostate cancer cell lines; However it has not been reported whether ARHI is regulated by miR-221 in breast cancer; To address whether ARHI is regulated by miR-221 in breast cancer cell lines the results in this study showed that a significant inverse correlation existed between ARHI and miR-221; The inhibition of miR-221 induced a significant upregulation of ARHI in MCF-7 cells; To prove a direct interaction between miR-221 and ARHI mRNA ARHI 3'UTR which includes the potential target site for miR-221 was cloned downstream of the luciferase reporter gene of the pMIR-REPORT vector to generate the pMIR-ARHI-3'UTR vector; The results confirmed a direct interaction of miR-221 with a target site on the 3'UTR of ARHI; This study demonstrated for the first time that the downregulation of ARHI in breast cancer cells could be regulated by miR-221;MicroRNA 221 and 222 have been shown to regulate ARHI expression negatively; Expression of ARHI and microRNA 221 and 222 was measured by real-time reverse transcription-polymerase chain reaction; Whether ARHI and microRNA 221 and 222 could be considered as biomarkers for disease progression in prostate cancer requires further investigation ;;progression prostate cancer;breast cancer;prostate cancer hsa-miR-21-5p DLG1 1.51084295924322 1.54551622173259e-34 -0.138873477942219 0.0841159694184806 miRNAWalker2_validate -0.189341396834391 3.40040140631564e-11 NA NA NA hsa-miR-21-5p DMD 1.51084295924322 1.54551622173259e-34 -1.6618166260325 2.70344741582688e-17 miRNAWalker2_validate -0.770128026941647 2.69472437841492e-28 NA NA NA hsa-miR-21-5p DOCK4 1.51084295924322 1.54551622173259e-34 -0.196572963974556 0.0730533705216442 miRNAWalker2_validate;miRTarBase -0.112445237932462 0.00485054593393611 NA NA NA hsa-miR-21-5p DOCK5 1.51084295924322 1.54551622173259e-34 -0.642812791721581 0.000979588907494083 miRNAWalker2_validate;miRTarBase -0.250802976494196 0.000433372119735922 NA NA NA hsa-miR-21-5p DOK6 1.51084295924322 1.54551622173259e-34 -1.91134260561967 2.47519280623105e-09 mirMAP -0.470056189254098 7.50851899469102e-05 NA NA NA hsa-miR-221-3p DPP8 1.12244756358612 3.48370308238012e-13 -0.274761779428937 0.0691793168762807 MirTarget -0.104806082957886 0.0242641125646373 NA NA NA hsa-miR-21-3p DSG1 -0.484219378716006 0.00299941098417659 -2.5596665573821 2.42887012547052e-08 MirTarget -0.636212998352284 4.09883972799604e-06 NA NA NA hsa-miR-21-5p DST 1.51084295924322 1.54551622173259e-34 -0.376077518150669 0.00154802384484121 mirMAP -0.192141258894703 8.41432997112948e-06 22403704 While the EDCs and estrogen similarly altered the expression of multiple microRNAs in MCF-7 cells including miR-21 differential patterns of microRNA expression were induced by DDT and BPA compared to estrogen breast cancer hsa-miR-21-5p DUSP10 1.51084295924322 1.54551622173259e-34 -1.25031678861182 9.23323170092562e-16 miRNAWalker2_validate;miRTarBase -0.33582473632279 6.72233513653455e-09 NA NA NA hsa-miR-21-3p DYNC1I1 -0.484219378716006 0.00299941098417659 1.66864494169359 1.46552904583527e-05 MirTarget -0.335277191056423 0.00382700919631388 NA NA NA hsa-miR-21-3p EBF3 -0.484219378716006 0.00299941098417659 1.73730919764558 2.69401589893511e-14 mirMAP -0.429577570871597 4.87631396235938e-10 NA NA NA hsa-miR-21-5p EGFR 1.51084295924322 1.54551622173259e-34 -1.01672920195188 6.69873186671412e-07 miRNAWalker2_validate;miRTarBase -0.440371363258275 3.21604130567409e-09 24198203;20113523;24012640;24331411;26563758;19597153;26026961;20048743 In radically resected NSCLC patients the expression levels of miR-21 10b in patients with EGFR mutation were much higher than those without mutation;Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;Further the expression of miR-21 is regulated by EGFR via the activation of β-catenin and AP-1; These data indicate that a feedback loop exists between miR-21 and EGFR; These results clarify a novel association between miR-21 and EGFR in the regulation of cancer cell progression;MiR 21 overexpression is associated with acquired resistance of EGFR TKI in non small cell lung cancer;Higher expression levels of miR-21 AmiR-27a and miR-218 detected in this study suggest potential roles of these miRNAs in primary resistance to EGFR-TKI in advanced NSCLC patients with EGFR exon 19 deletion mutations;MiR 21 is an EGFR regulated anti apoptotic factor in lung cancer in never smokers; The changes in expression of some of these miRNAs including miR-21 were more remarkable in cases with EGFR mutations than in those without these mutations; In the never-smoker-derived lung adenocarcinoma cell line H3255 with mutant EGFR and high levels of p-EGFR and miR-21 antisense inhibition of miR-21 enhanced AG1478-induced apoptosis; In a never-smoker-derived adenocarcinoma cell line H441 with wild-type EGFR the antisense miR-21 not only showed the additive effect with AG1478 but also induced apoptosis by itself; These results suggest that aberrantly increased expression of miR-21 which is enhanced further by the activated EGFR signaling pathway plays a significant role in lung carcinogenesis in never-smokers as well as in smokers and is a potential therapeutic target in both EGFR-mutant and wild-type cases;Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR mutated lung cancer via nickel induced microRNA 21 expression;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status ;;progression;drug resistance;staging;drug resistance;tumorigenesis;; lung squamous cell cancer;glioblastoma;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;lung cancer;lung cancer;glioblastoma hsa-miR-21-5p EIF1AX 1.51084295924322 1.54551622173259e-34 -0.291968132466788 0.00113929910702412 miRNATAP -0.23005485673981 5.66462566420026e-13 NA NA NA hsa-miR-221-3p EIF4E3 1.12244756358612 3.48370308238012e-13 -1.65776517548242 5.25920384886669e-14 miRNATAP -0.356271018925111 2.54218264646758e-07 NA NA NA hsa-miR-21-5p EIF4EBP2 1.51084295924322 1.54551622173259e-34 -0.113333423080928 0.274925870028417 miRNAWalker2_validate -0.25252138439241 7.37948303219334e-12 NA NA NA hsa-miR-21-5p EIF5 1.51084295924322 1.54551622173259e-34 -0.718800486296156 2.12840695796032e-16 miRNAWalker2_validate -0.256656216236592 9.41542312025708e-16 NA NA NA hsa-miR-21-5p ELF2 1.51084295924322 1.54551622173259e-34 -0.389705823694809 5.48578362990734e-07 MirTarget;miRNATAP -0.166817795102023 3.10695031389035e-09 NA NA NA hsa-miR-21-3p ELK4 -0.484219378716006 0.00299941098417659 0.23519596686873 0.0267189511596387 mirMAP -0.106868781896012 0.000704209163548549 NA NA NA hsa-miR-21-5p ENTPD4 1.51084295924322 1.54551622173259e-34 -0.411856151063958 0.000793591455053331 mirMAP -0.139222165419153 0.00192356522289834 NA NA NA hsa-miR-21-5p EPM2A 1.51084295924322 1.54551622173259e-34 -1.15770706080728 3.57362529045184e-22 miRNAWalker2_validate -0.563090697791134 5.72879488783456e-42 NA NA NA hsa-miR-21-3p EPM2AIP1 -0.484219378716006 0.00299941098417659 -0.295909832927366 0.00243576222358727 MirTarget -0.152314607868055 1.20779966036571e-07 NA NA NA hsa-miR-21-5p EPM2AIP1 1.51084295924322 1.54551622173259e-34 -0.295909832927366 0.00243576222358727 mirMAP -0.279763209592185 4.45838175922043e-16 NA NA NA hsa-miR-221-3p ERCC4 1.12244756358612 3.48370308238012e-13 -0.398490625556519 0.000316330847829454 MirTarget -0.235928816218724 1.36429369950774e-12 NA NA NA hsa-miR-21-3p ESR1 -0.484219378716006 0.00299941098417659 -4.11885094897542 1.63160126578307e-28 mirMAP -0.313737544889119 0.00839097080849286 25969534;19264808;25337203;21131358 Dehydroepiandrosterone Activation of G protein coupled Estrogen Receptor Rapidly Stimulates MicroRNA 21 Transcription in Human Hepatocellular Carcinoma Cells;miR-21 is higher in ER alpha positive than negative tumors but no one has examined how estradiol E2 regulates miR-21 in breast cancer cells; The E2-induced reduction in miR-21 was inhibited by 4-hydroxytamoxifen 4-OHT ICI 182 780 Faslodex and siRNA ER alpha indicating that the suppression is ER alpha-mediated; ER alpha and ER beta agonists PPT and DPN inhibited and 4-OHT increased miR-21 expression; These results are the first to demonstrate that E2 represses the expression of an oncogenic miRNA miR-21 by activating estrogen receptor in MCF-7 cells;Odds ratios ORs showed that miR-21 expression was closely associated with estrogen receptor ER progesterone receptor PR lymph node metastasis histological grade Her2/neu;Induction of miR 21 by retinoic acid in estrogen receptor positive breast carcinoma cells: biological correlates and molecular targets ;;metastasis; liver cancer;breast cancer;breast cancer;breast cancer hsa-miR-221-3p ESR1 1.12244756358612 3.48370308238012e-13 -4.11885094897542 1.63160126578307e-28 miRNAWalker2_validate;miRNATAP -1.10370333584046 3.63055649331572e-21 25483016 HBx protein induced upregulation of microRNA 221 promotes aberrant proliferation in HBV‑related hepatocellular carcinoma by targeting estrogen receptor α liver cancer hsa-miR-21-5p ESYT2 1.51084295924322 1.54551622173259e-34 -0.172147082999767 0.039027418424603 miRNAWalker2_validate;MirTarget -0.121583148574807 5.55345929002504e-05 NA NA NA hsa-miR-221-3p ETS1 1.12244756358612 3.48370308238012e-13 -0.888146453921594 7.30600721510989e-10 miRTarBase;miRNATAP -0.163620716574743 0.000282330030340872 21711453 To close the loop we demonstrate ETS-1 as a direct target of miR-222 but not miR-221 showing the novel option of their uncoupled functions melanoma hsa-miR-221-3p ETS2 1.12244756358612 3.48370308238012e-13 -1.75880257972087 1.4083094059301e-32 miRNATAP -0.357161643234593 8.61994145380732e-14 NA NA NA hsa-miR-21-5p EXOC5 1.51084295924322 1.54551622173259e-34 -0.381869800654396 2.14629338906463e-06 miRNAWalker2_validate -0.199036570313502 5.77507937444715e-12 NA NA NA hsa-miR-21-5p FAM107B 1.51084295924322 1.54551622173259e-34 -0.783707675483699 1.16417578511053e-13 miRNATAP -0.235074428188822 1.61942879549264e-09 NA NA NA hsa-miR-21-5p FAM126B 1.51084295924322 1.54551622173259e-34 -0.636777402416019 9.26093586171305e-12 miRNAWalker2_validate -0.284805117357217 2.09374309898492e-17 NA NA NA hsa-miR-221-3p FAM13A 1.12244756358612 3.48370308238012e-13 -2.57744428571964 2.57406538004678e-26 miRNATAP -0.57412851838564 1.18945467601914e-13 NA NA NA hsa-miR-21-5p FAM46A 1.51084295924322 1.54551622173259e-34 -1.45135702962017 1.01521313725168e-19 miRNAWalker2_validate;miRNATAP -0.306527061274061 3.82955649227577e-07 NA NA NA hsa-miR-21-5p FAM63B 1.51084295924322 1.54551622173259e-34 -0.544563365894281 0.0024060920267518 miRNATAP -0.211932275839677 0.00123083612197724 NA NA NA hsa-miR-21-5p FAS 1.51084295924322 1.54551622173259e-34 -1.01888565147264 1.11378826495697e-07 miRNAWalker2_validate -0.345881974093154 8.950367188801e-07 24710931 miR 21 targets Fas ligand mediated apoptosis in breast cancer cell line MCF 7 breast cancer hsa-miR-21-5p FBXL17 1.51084295924322 1.54551622173259e-34 -0.248708925063077 0.00488542308997094 miRNAWalker2_validate;miRNATAP -0.189629901093539 2.1369479396319e-09 NA NA NA hsa-miR-21-5p FBXO28 1.51084295924322 1.54551622173259e-34 -0.503712735174914 1.26784281427187e-10 MirTarget -0.193267594867859 1.05986699358805e-11 NA NA NA hsa-miR-21-5p FBXO3 1.51084295924322 1.54551622173259e-34 -0.544237692707206 2.24798669719614e-09 miRNAWalker2_validate -0.271169014440239 7.74693142357381e-17 NA NA NA hsa-miR-21-5p FERMT2 1.51084295924322 1.54551622173259e-34 -1.17471907439772 6.50761494287311e-25 miRNAWalker2_validate -0.471357960010248 1.37679112814546e-30 NA NA NA hsa-miR-221-3p FERMT2 1.12244756358612 3.48370308238012e-13 -1.17471907439772 6.50761494287311e-25 MirTarget;miRNATAP -0.153189097329362 3.36111787442557e-05 NA NA NA hsa-miR-21-5p FGD4 1.51084295924322 1.54551622173259e-34 -1.15544171645474 1.45947973849956e-12 MirTarget -0.493369028543639 6.13846194813968e-17 NA NA NA hsa-miR-21-3p FGF2 -0.484219378716006 0.00299941098417659 -1.08625533231299 0.000324809942833161 mirMAP -0.240197113439825 0.00811114129230132 NA NA NA hsa-miR-21-5p FGF2 1.51084295924322 1.54551622173259e-34 -1.08625533231299 0.000324809942833161 mirMAP -0.443381765546842 5.91228766084995e-05 NA NA NA hsa-miR-21-5p FILIP1L 1.51084295924322 1.54551622173259e-34 -1.18591508212985 2.30526943213347e-11 miRNAWalker2_validate -0.219368868643445 0.000918628151324769 NA NA NA hsa-miR-221-3p FKBP5 1.12244756358612 3.48370308238012e-13 -0.232073250029758 0.19420333201796 mirMAP -0.219251177999315 5.95058700008135e-05 NA NA NA hsa-miR-21-5p FMR1 1.51084295924322 1.54551622173259e-34 0.0205799660201702 0.848215525236684 miRNAWalker2_validate -0.179786227316967 3.48177677419723e-06 NA NA NA hsa-miR-221-3p FNDC3A 1.12244756358612 3.48370308238012e-13 -0.995884475839302 2.03718393267477e-13 MirTarget;miRNATAP -0.14342992972706 0.000806093199924562 NA NA NA hsa-miR-221-3p FOS 1.12244756358612 3.48370308238012e-13 -3.63859914445361 9.27794861825719e-37 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.561899061238752 3.60354241869353e-09 NA NA NA hsa-miR-21-5p FOXN3 1.51084295924322 1.54551622173259e-34 -0.433290741984388 5.6274900890093e-05 miRNAWalker2_validate;mirMAP -0.363687314557807 3.15767982646235e-22 NA NA NA hsa-miR-21-5p FOXP2 1.51084295924322 1.54551622173259e-34 -3.24994941369723 1.71142859920958e-14 mirMAP -0.735827260635865 3.46535182735824e-06 NA NA NA hsa-miR-21-5p FREM2 1.51084295924322 1.54551622173259e-34 -5.80688485224769 4.9991869723566e-26 mirMAP -1.22416237431968 6.30716303810603e-09 NA NA NA hsa-miR-21-5p FRMD3 1.51084295924322 1.54551622173259e-34 0.199205669298484 0.521900499692411 MirTarget -0.384481377544254 0.000675378904605147 NA NA NA hsa-miR-221-3p FXN 1.12244756358612 3.48370308238012e-13 -1.37523749344236 9.95432558430183e-29 MirTarget -0.155802968185431 0.000122146660224215 NA NA NA hsa-miR-21-5p FZD4 1.51084295924322 1.54551622173259e-34 -0.346477770504521 0.0109747315056824 mirMAP -0.321725677881532 3.91700387140416e-11 NA NA NA hsa-miR-221-3p GAB1 1.12244756358612 3.48370308238012e-13 -0.316010954422049 0.00456197149339329 MirTarget;miRNATAP -0.141495019686334 3.2987590560242e-05 NA NA NA hsa-miR-21-5p GFOD1 1.51084295924322 1.54551622173259e-34 -0.739136473829968 1.54144336609472e-06 mirMAP -0.365376621578094 4.83300398323549e-11 NA NA NA hsa-miR-21-5p GLCCI1 1.51084295924322 1.54551622173259e-34 -0.285824127662563 0.0400950675736783 miRNAWalker2_validate;MirTarget;miRNATAP -0.421184063560818 6.28784287543577e-18 NA NA NA hsa-miR-21-5p GLYR1 1.51084295924322 1.54551622173259e-34 -0.173595165539688 0.0275427557335278 miRNATAP -0.104856851434877 0.000236785879216891 NA NA NA hsa-miR-221-3p GLYR1 1.12244756358612 3.48370308238012e-13 -0.173595165539688 0.0275427557335278 miRNAWalker2_validate -0.10587081673189 9.80351392221864e-06 NA NA NA hsa-miR-21-5p GNAQ 1.51084295924322 1.54551622173259e-34 -0.387863258052234 5.35620148383708e-06 miRNAWalker2_validate -0.246469163664991 3.51832080720891e-16 NA NA NA hsa-miR-21-5p GNE 1.51084295924322 1.54551622173259e-34 -1.82350791069008 3.50187061744317e-25 miRNAWalker2_validate -0.756130062299966 3.40005906023502e-33 NA NA NA hsa-miR-21-5p GOLGA4 1.51084295924322 1.54551622173259e-34 -0.24392104493269 0.0186334222534644 miRNAWalker2_validate -0.245719613261825 3.01269574706493e-11 NA NA NA hsa-miR-21-5p GPAM 1.51084295924322 1.54551622173259e-34 -0.472844151955403 0.0637965177079971 miRNAWalker2_validate;mirMAP -0.627076677904986 5.37674526051361e-12 NA NA NA hsa-miR-221-3p GPM6A 1.12244756358612 3.48370308238012e-13 -4.84536564920804 3.01663369835863e-39 miRNATAP -0.872916127861325 8.87832692814009e-13 NA NA NA hsa-miR-21-3p GPRASP2 -0.484219378716006 0.00299941098417659 -0.64634103964299 3.73116290903046e-06 MirTarget -0.131512399515082 0.00175432439901039 NA NA NA hsa-miR-21-5p GRAMD3 1.51084295924322 1.54551622173259e-34 -0.644653008314935 1.85257840666131e-12 MirTarget -0.269437888121112 3.43421721008377e-16 NA NA NA hsa-miR-21-3p GTF2A1 -0.484219378716006 0.00299941098417659 -0.686715241762695 0.00535884343767192 MirTarget -0.185189066425539 0.0121330641309006 NA NA NA hsa-miR-21-5p GTF2A1 1.51084295924322 1.54551622173259e-34 -0.686715241762695 0.00535884343767192 miRNAWalker2_validate -0.432330601899388 1.32918511417795e-06 NA NA NA hsa-miR-21-5p GTF2I 1.51084295924322 1.54551622173259e-34 -0.00748089243032002 0.968048343679462 miRNAWalker2_validate -0.228506436155094 0.000758135102357862 NA NA NA hsa-miR-221-3p GUCY1A2 1.12244756358612 3.48370308238012e-13 0.0715677649443884 0.676881020366457 MirTarget -0.213204118706513 4.72670178076662e-05 NA NA NA hsa-miR-21-5p GXYLT1 1.51084295924322 1.54551622173259e-34 -0.349240174864085 3.06569030604751e-05 mirMAP -0.165206324251823 4.93697672660951e-08 NA NA NA hsa-miR-21-5p HBP1 1.51084295924322 1.54551622173259e-34 -0.357103377571568 1.81649948774568e-05 miRNATAP -0.235889270910882 1.4074266579388e-15 26282675 MicroRNA 21 is a potential link between non alcoholic fatty liver disease and hepatocellular carcinoma via modulation of the HBP1 p53 Srebp1c pathway; Further studies revealed that Hbp1 was a novel target of microRNA-21 and a transcriptional activator of p53 liver cancer hsa-miR-21-5p HECTD1 1.51084295924322 1.54551622173259e-34 -0.512253958763233 2.26630270647066e-07 miRNAWalker2_validate -0.378550769030999 1.41086458902319e-28 NA NA NA hsa-miR-221-3p HECTD1 1.12244756358612 3.48370308238012e-13 -0.512253958763233 2.26630270647066e-07 miRNAWalker2_validate -0.122744599499136 6.07660978879542e-05 NA NA NA hsa-miR-21-5p HHIP 1.51084295924322 1.54551622173259e-34 -5.86031709546153 8.91627452602351e-41 mirMAP -1.12169197316927 1.01232747513074e-10 NA NA NA hsa-miR-21-5p HIPK1 1.51084295924322 1.54551622173259e-34 -0.16031440103832 0.127196102040304 mirMAP -0.108627540305843 0.00448750637888426 NA NA NA hsa-miR-221-3p HIPK1 1.12244756358612 3.48370308238012e-13 -0.16031440103832 0.127196102040304 MirTarget;miRNATAP -0.114800628703969 0.000350703360286203 NA NA NA hsa-miR-21-3p HIPK2 -0.484219378716006 0.00299941098417659 -0.239689841914744 0.0838927941290475 mirMAP -0.17489744414667 2.03920224734065e-05 NA NA NA hsa-miR-221-3p HIPK2 1.12244756358612 3.48370308238012e-13 -0.239689841914744 0.0838927941290475 miRNATAP -0.20708116108492 9.03305228275801e-07 NA NA NA hsa-miR-21-5p HIPK3 1.51084295924322 1.54551622173259e-34 -1.50429429958896 1.36124385011385e-07 MirTarget -0.578663959515645 2.86739236558473e-08 NA NA NA hsa-miR-221-3p HIPK3 1.12244756358612 3.48370308238012e-13 -1.50429429958896 1.36124385011385e-07 MirTarget -0.301787858496408 0.000683773574086624 NA NA NA hsa-miR-221-3p HIVEP1 1.12244756358612 3.48370308238012e-13 -1.10787940806176 3.2752718772151e-16 miRNAWalker2_validate -0.20729234794859 1.32454788862852e-06 NA NA NA hsa-miR-221-3p HMBOX1 1.12244756358612 3.48370308238012e-13 -0.648299686508313 1.92402108459881e-07 MirTarget;miRNATAP -0.141745318893312 0.000243612435054518 NA NA NA hsa-miR-221-3p HOOK1 1.12244756358612 3.48370308238012e-13 -1.00940473494578 9.98206390806586e-11 MirTarget -0.208587200086646 1.88071993726591e-05 NA NA NA hsa-miR-21-5p HPS5 1.51084295924322 1.54551622173259e-34 -1.54497064795797 1.96024564670947e-23 miRNAWalker2_validate -0.358324432918708 1.04352822729288e-09 NA NA NA hsa-miR-21-5p HS3ST3B1 1.51084295924322 1.54551622173259e-34 -2.60592423161066 9.57213132909845e-18 miRNAWalker2_validate -0.976241117224746 1.34764029616548e-18 NA NA NA hsa-miR-21-3p HSD17B11 -0.484219378716006 0.00299941098417659 -0.50760380562319 0.000113495231116008 MirTarget -0.112459705192718 0.00434960969887362 NA NA NA hsa-miR-21-5p HSD17B4 1.51084295924322 1.54551622173259e-34 -0.640677186239444 9.56450055730174e-06 MirTarget -0.49064613873703 3.4002637267839e-22 NA NA NA hsa-miR-21-5p IDS 1.51084295924322 1.54551622173259e-34 -0.546753809841531 7.44193151771148e-07 mirMAP -0.101285486064705 0.0129830877855469 NA NA NA hsa-miR-21-5p IL6R 1.51084295924322 1.54551622173259e-34 -0.197357669964791 0.382548993653367 MirTarget;miRNATAP -0.536139043119449 2.87865737326418e-11 NA NA NA hsa-miR-21-5p IL6ST 1.51084295924322 1.54551622173259e-34 -1.91925864959166 1.59476897276323e-08 mirMAP -0.643707080430308 2.44682283240179e-07 NA NA NA hsa-miR-21-5p ING3 1.51084295924322 1.54551622173259e-34 -0.222016692366606 0.0136456256313062 MirTarget -0.175389774794838 5.94720107713985e-08 NA NA NA hsa-miR-221-3p INSIG1 1.12244756358612 3.48370308238012e-13 -0.982451848864065 0.000118571254506076 MirTarget;miRNATAP -0.66232636123501 2.64292654726766e-18 NA NA NA hsa-miR-21-5p INSR 1.51084295924322 1.54551622173259e-34 -0.278293402111066 0.00785689311731476 mirMAP -0.133506275914425 0.00045620211593194 24804226;27220494;21618027 Downregulation of miR-21 in radioresistant NSCLC A549 cells inhibited the colony-forming ability and proliferation of A549 cells after IR; Moreover silencing miR-21 enhanced apoptosis of A549 cells induced by IR accompanied by decreased phosphorylated-Akt protein level; However PI3K activator IGF-1 reversed suppression of phosphorylated-Akt protein level and promotion of apoptosis of A549 cells after IR caused by miR-21 knockdown; Silencing miR-21 in radioresistant NSCLC A549 cells sensitized them to IR by inhibiting cell proliferation and enhancing cell apoptosis through inhibition of PI3K/Akt signaling pathway;In addition we also demonstrated that miR-21 confers decreased autophagy in cervical cancer cells after IR via the Akt-mTOR signaling pathway;In this study we found that miR-21 expression was upregulated in response to ionizing radiation IR in U251 cells which suggested that miR-21 could be involved in the response of U251 cells to radiation; We showed that a miR-21 inhibitor enhanced IR-induced glioblastoma cell growth arrest and increased the level of apoptosis which was probably caused by abrogation of the G2-M arrest induced by IR ;;drug resistance lung squamous cell cancer;cervical and endocervical cancer;glioblastoma hsa-miR-21-5p INTS6 1.51084295924322 1.54551622173259e-34 -0.650926189132886 9.71819582580471e-11 MirTarget -0.226990475333205 6.77043846342216e-10 NA NA NA hsa-miR-21-5p IPP 1.51084295924322 1.54551622173259e-34 -0.117789097974411 0.331754038056184 miRNAWalker2_validate -0.149850091403392 0.000668566581290115 NA NA NA hsa-miR-21-5p IREB2 1.51084295924322 1.54551622173259e-34 -0.370291061083076 1.38083203196362e-05 miRNAWalker2_validate -0.224298460304271 1.56840495630729e-13 NA NA NA hsa-miR-221-3p IRF2 1.12244756358612 3.48370308238012e-13 -0.488730264470989 9.63225888653794e-08 miRNATAP -0.127838277330377 6.17003025135955e-06 24607843 Survival in patients with high risk prostate cancer is predicted by miR 221 which regulates proliferation apoptosis and invasion of prostate cancer cells by inhibiting IRF2 and SOCS3; miR-221 directly inhibits the expression of SOCS3 and IRF2 two oncogenes that negatively regulate this signaling pathway poor survival prostate cancer hsa-miR-21-5p ITGA1 1.51084295924322 1.54551622173259e-34 -0.675046465429795 1.83477212641831e-07 mirMAP -0.324725552717668 3.57684105241524e-12 NA NA NA hsa-miR-221-3p ITGB3 1.12244756358612 3.48370308238012e-13 -0.984722780911358 0.000225186373225592 MirTarget -0.386235530184791 2.395985878903e-06 NA NA NA hsa-miR-21-3p JMY -0.484219378716006 0.00299941098417659 -0.0723925290319816 0.482620332419229 mirMAP -0.114846133835329 0.00016646519838069 NA NA NA hsa-miR-21-5p JMY 1.51084295924322 1.54551622173259e-34 -0.0723925290319816 0.482620332419229 miRNAWalker2_validate;mirMAP -0.167929539107582 6.27891328902698e-06 NA NA NA hsa-miR-21-5p KBTBD6 1.51084295924322 1.54551622173259e-34 -0.188967726777397 0.0378562517960693 miRNAWalker2_validate;MirTarget -0.212823281956273 5.20842909132223e-11 NA NA NA hsa-miR-21-5p KBTBD7 1.51084295924322 1.54551622173259e-34 -0.935413933152499 3.50066588121242e-06 miRNAWalker2_validate -0.541976693322973 6.40797115655026e-14 NA NA NA hsa-miR-221-3p KBTBD8 1.12244756358612 3.48370308238012e-13 -0.535277103367331 0.00302109600455283 MirTarget -0.196397871725741 0.00040178822828246 NA NA NA hsa-miR-21-5p KCNA3 1.51084295924322 1.54551622173259e-34 -1.97421353774174 1.82123288049626e-13 miRNATAP -0.299637746508877 0.00295778536619722 NA NA NA hsa-miR-221-3p KCNK2 1.12244756358612 3.48370308238012e-13 -1.05245883813564 0.004513177443484 MirTarget -0.341457459965925 0.00279992007191986 NA NA NA hsa-miR-21-5p KIAA0355 1.51084295924322 1.54551622173259e-34 0.0309935715483438 0.728406529714885 MirTarget -0.149906051615669 3.03724363617167e-06 NA NA NA hsa-miR-221-3p KIAA0430 1.12244756358612 3.48370308238012e-13 -0.22181354906702 0.00175734986558445 MirTarget;miRNATAP -0.114871770181451 8.77375299524028e-08 NA NA NA hsa-miR-21-3p KIAA1462 -0.484219378716006 0.00299941098417659 1.43292602496162 9.79261879524332e-18 MirTarget -0.18095854328301 0.000474113126880573 NA NA NA hsa-miR-21-5p KIAA1671 1.51084295924322 1.54551622173259e-34 -0.839733934948399 1.10952867050449e-11 mirMAP -0.164845382686981 0.000345737631950021 NA NA NA hsa-miR-21-5p KLF12 1.51084295924322 1.54551622173259e-34 -0.863221902725629 5.4142350807271e-09 mirMAP -0.545651132892819 4.906175473079e-26 NA NA NA hsa-miR-21-3p KLF13 -0.484219378716006 0.00299941098417659 1.11571195583237 1.69897534012305e-15 mirMAP -0.134235765039176 0.00189492202587126 NA NA NA hsa-miR-21-5p KLF3 1.51084295924322 1.54551622173259e-34 -0.612085068754795 1.13963384347611e-13 miRNATAP -0.230378723364208 1.47789299053091e-14 NA NA NA hsa-miR-21-5p KLF6 1.51084295924322 1.54551622173259e-34 -1.43852959018658 4.46638206050043e-20 miRNATAP -0.265009732469504 8.49803425647928e-06 NA NA NA hsa-miR-21-5p KLF9 1.51084295924322 1.54551622173259e-34 -1.35905833834713 1.22932810880238e-12 miRNAWalker2_validate -0.607422599570453 1.16785059060082e-18 NA NA NA hsa-miR-221-3p KLF9 1.12244756358612 3.48370308238012e-13 -1.35905833834713 1.22932810880238e-12 miRNAWalker2_validate -0.558617680931615 2.50451535003869e-22 NA NA NA hsa-miR-21-5p KLHL15 1.51084295924322 1.54551622173259e-34 -1.43616317329618 4.62856180062188e-29 miRNAWalker2_validate;miRNATAP -0.656684809309307 3.29358691062809e-49 NA NA NA hsa-miR-21-5p KLHL24 1.51084295924322 1.54551622173259e-34 -0.401981621711891 5.62016159465388e-05 miRNAWalker2_validate -0.302519889785232 8.17078443675782e-18 NA NA NA hsa-miR-21-5p KLHL4 1.51084295924322 1.54551622173259e-34 -1.08539693977576 1.31560067160153e-06 mirMAP -0.175487393215786 0.0345586631691532 NA NA NA hsa-miR-221-3p KLHL8 1.12244756358612 3.48370308238012e-13 -0.860556861719106 3.7235566785704e-09 miRNAWalker2_validate -0.331993361082056 7.49559007688179e-14 NA NA NA hsa-miR-221-3p KPNA6 1.12244756358612 3.48370308238012e-13 -0.221388310063308 0.00128672982834015 miRNAWalker2_validate -0.119204640480054 9.51436736094924e-09 NA NA NA hsa-miR-21-3p L2HGDH -0.484219378716006 0.00299941098417659 -0.858608570256839 3.35757853795555e-11 MirTarget -0.219064272169924 1.87618343459461e-08 NA NA NA hsa-miR-21-5p LAMP2 1.51084295924322 1.54551622173259e-34 -0.141489314494891 0.203338123114718 miRNAWalker2_validate -0.136266688445851 0.000736718604940198 NA NA NA hsa-miR-21-5p LATS1 1.51084295924322 1.54551622173259e-34 -0.730797585786345 4.24866694321044e-05 miRNAWalker2_validate -0.259052381224193 7.34088380250575e-05 25769949 miR 21 modulates resistance of HR HPV positive cervical cancer cells to radiation through targeting LATS1; By using dual luciferase assay we verified a binding site between miR-21 and 3'-UTR of large tumor suppressor kinase 1 LATS1; Through direct binding miR-21 can regulate LATS1 expression in cervical cancer cells; LATS1 overexpression can reverse miR-21 induced higher colony formation rate and also reduced miR-21 induced S phase accumulation and G2/M phase block reduction under radiation treatment drug resistance cervical and endocervical cancer hsa-miR-21-5p LCOR 1.51084295924322 1.54551622173259e-34 -0.828225234134718 0.000381975258357077 mirMAP -0.300233861296471 0.000433982911945699 NA NA NA hsa-miR-21-5p LCORL 1.51084295924322 1.54551622173259e-34 -0.465224089674271 0.000125378997414632 miRNAWalker2_validate;miRNATAP -0.169008923460901 0.000136559612734448 NA NA NA hsa-miR-21-5p LEMD3 1.51084295924322 1.54551622173259e-34 -0.241034061384027 0.00152124310234342 miRNATAP -0.124267537760017 6.15901201616457e-06 NA NA NA hsa-miR-21-5p LIFR 1.51084295924322 1.54551622173259e-34 -3.54791005337671 1.62449725318617e-42 miRNAWalker2_validate -0.970873248845757 3.02875243005404e-22 NA NA NA hsa-miR-221-3p LIFR 1.12244756358612 3.48370308238012e-13 -3.54791005337671 1.62449725318617e-42 miRNATAP -0.543309890221884 4.42342545438935e-10 NA NA NA hsa-miR-21-5p LIN7C 1.51084295924322 1.54551622173259e-34 -0.778211251236792 5.38883335125652e-24 miRNAWalker2_validate -0.316945336013872 1.32056596586162e-30 NA NA NA hsa-miR-21-5p LMBR1 1.51084295924322 1.54551622173259e-34 -0.126617891799222 0.154766250119006 miRNAWalker2_validate -0.12248170979845 0.000141864653574036 NA NA NA hsa-miR-21-5p LPA 1.51084295924322 1.54551622173259e-34 -3.96486166434916 7.78567900365653e-23 MirTarget -1.32367982780015 6.65823633941695e-19 26098771 DNA microarray and real-time PCR analyses further demonstrated that LPA up-regulated the oncomiR miR-21 through an LPA1/Pi3K/ZEB1-dependent mechanism breast cancer hsa-miR-21-5p LPIN2 1.51084295924322 1.54551622173259e-34 -1.28920874369498 9.96607683916703e-16 mirMAP -0.32154832087112 8.22951864870956e-08 NA NA NA hsa-miR-221-3p LRP6 1.12244756358612 3.48370308238012e-13 -0.374410866893442 0.0013487864538849 miRNAWalker2_validate -0.100566445726281 0.00520193003801261 NA NA NA hsa-miR-21-5p LRRC55 1.51084295924322 1.54551622173259e-34 -3.40869096246176 2.95454085746275e-31 mirMAP -0.782400175562664 4.02887388179652e-12 NA NA NA hsa-miR-221-3p LRRCC1 1.12244756358612 3.48370308238012e-13 0.0427198654794261 0.831958818301209 MirTarget -0.20232256254731 0.00103300782777049 NA NA NA hsa-miR-21-5p LRRFIP1 1.51084295924322 1.54551622173259e-34 -0.587622691682438 1.09608882717065e-10 miRNAWalker2_validate;miRTarBase -0.17386208053287 2.08191255544983e-07 19559015 MicroRNA 21 targets LRRFIP1 and contributes to VM 26 resistance in glioblastoma multiforme; We further identified and validated LRRFIP1 whose product is an inhibitor of NF-kappaB signaling as a direct target gene of miR-21 drug resistance glioblastoma hsa-miR-21-3p LYNX1 -0.484219378716006 0.00299941098417659 0.0416489629136718 0.869448587301387 mirMAP -0.267247585293623 0.000381996548631173 NA NA NA hsa-miR-21-5p LYRM7 1.51084295924322 1.54551622173259e-34 -0.285114105890549 0.000746869763807281 miRNAWalker2_validate -0.246685558442981 1.19117649166692e-16 NA NA NA hsa-miR-21-5p LZTFL1 1.51084295924322 1.54551622173259e-34 -1.00294712153243 8.04932478902966e-12 MirTarget -0.342375510020902 1.99016002722285e-10 NA NA NA hsa-miR-21-5p MAP2K3 1.51084295924322 1.54551622173259e-34 -1.21754800169498 4.92442767488047e-26 MirTarget -0.296630051548559 1.21863335298365e-11 24112539 The 3'-untranslated region 3'-UTR of MAP2K3 combined with miR-21 was experimentally verified by a miRNA luciferase reporter approach; More importantly the repression of MAP2K3 was inversely correlated with the expression of miR-21 in HCC; Further study demonstrated that the MAP2K3 was a novel direct target of miR-21 which was experimentally validated by a miRNA luciferase reporter approach; In HepG2 cells inhibition of miR-21 expression with an adenoviral miR-21 sponge vector profoundly suppressed cell proliferation by up-regulating MAP2K3 expression at both mRNA and protein levels; These results provide a clinical evidence that MAP2K3 may be a tumor repressor gene and it is a direct target of miR-21 in HCC indicating an underlying mechanism by which miR-21 is able to directly target MAP2K3 and inhibit its expression during the carcinogenesis of HCC at both transcriptional and post-translational levels tumorigenesis liver cancer hsa-miR-21-5p MAP3K2 1.51084295924322 1.54551622173259e-34 -0.770538748173389 6.13734867546361e-08 miRNAWalker2_validate;mirMAP -0.282876404225472 5.34149880909856e-08 NA NA NA hsa-miR-221-3p MAP3K2 1.12244756358612 3.48370308238012e-13 -0.770538748173389 6.13734867546361e-08 MirTarget -0.239445403358139 4.60022991721426e-08 NA NA NA hsa-miR-221-3p MAPK10 1.12244756358612 3.48370308238012e-13 -1.09210524801043 3.20431274629696e-05 miRNATAP -0.345546531666561 1.95748420882985e-05 NA NA NA hsa-miR-21-5p MATN2 1.51084295924322 1.54551622173259e-34 -0.692218006755673 0.0026937518103409 miRNATAP -0.439006230822363 1.45013185590069e-07 NA NA NA hsa-miR-21-5p MBLAC2 1.51084295924322 1.54551622173259e-34 -0.651946233751667 4.55219397233215e-07 MirTarget -0.420975094961421 2.6165956891375e-20 NA NA NA hsa-miR-21-3p MBNL3 -0.484219378716006 0.00299941098417659 -0.597245834128655 0.0142379871483953 MirTarget -0.337276960903564 2.98910638546252e-06 NA NA NA hsa-miR-21-5p MBNL3 1.51084295924322 1.54551622173259e-34 -0.597245834128655 0.0142379871483953 MirTarget;mirMAP -0.4533266262411 2.65298361951513e-07 NA NA NA hsa-miR-21-5p MBP 1.51084295924322 1.54551622173259e-34 -0.401034568218827 0.00152582531127159 mirMAP -0.141180976134 0.00226478841976692 NA NA NA hsa-miR-21-5p MBTPS2 1.51084295924322 1.54551622173259e-34 -0.319653122535307 0.000361871015724243 miRNATAP -0.201114962301786 4.01285459715525e-10 NA NA NA hsa-miR-21-5p MCC 1.51084295924322 1.54551622173259e-34 -2.36915505634884 5.46995786737825e-22 mirMAP -0.582634900768505 3.39690430566654e-10 NA NA NA hsa-miR-21-5p MEF2A 1.51084295924322 1.54551622173259e-34 -0.4924154435107 8.54172101119762e-06 miRNAWalker2_validate -0.209572178655664 1.86913254606396e-07 NA NA NA hsa-miR-21-5p MEGF9 1.51084295924322 1.54551622173259e-34 -0.767121149729698 4.19664132638782e-10 miRNAWalker2_validate;mirMAP;miRNATAP -0.33278690568075 6.687300960684e-14 NA NA NA hsa-miR-221-3p MEGF9 1.12244756358612 3.48370308238012e-13 -0.767121149729698 4.19664132638782e-10 MirTarget;miRNATAP -0.153028854885442 6.49570820433605e-05 NA NA NA hsa-miR-221-3p MFN2 1.12244756358612 3.48370308238012e-13 -0.60468815586085 4.04345111138286e-11 miRNAWalker2_validate -0.207769422705299 1.03150022618557e-13 NA NA NA hsa-miR-21-3p MIA3 -0.484219378716006 0.00299941098417659 -0.209214323545272 0.0718582390655248 MirTarget -0.200738979798704 3.72684342597484e-09 NA NA NA hsa-miR-21-5p MIB1 1.51084295924322 1.54551622173259e-34 -0.0264398771590288 0.808071482948044 miRNAWalker2_validate;mirMAP -0.125529680447218 0.00148022035997289 NA NA NA hsa-miR-221-3p MIER3 1.12244756358612 3.48370308238012e-13 -0.289689451855984 0.00044472169352925 MirTarget;miRNATAP -0.110138217681738 1.24570866635589e-05 NA NA NA hsa-miR-21-5p MKNK2 1.51084295924322 1.54551622173259e-34 -0.0576836459714736 0.518912431453789 miRNAWalker2_validate -0.119547802728919 0.000218174804414907 NA NA NA hsa-miR-21-3p MMRN2 -0.484219378716006 0.00299941098417659 -0.0535902644152353 0.692073107856265 MirTarget -0.129101959174448 0.00131071722248291 NA NA NA hsa-miR-21-5p MOAP1 1.51084295924322 1.54551622173259e-34 -0.300255392027902 0.000344464463896101 miRNAWalker2_validate -0.177136746595483 4.29370776021504e-09 NA NA NA hsa-miR-21-5p MON2 1.51084295924322 1.54551622173259e-34 -0.365012009899068 9.80646639998691e-06 miRNAWalker2_validate;MirTarget;mirMAP -0.220754527266474 6.21137821016038e-14 NA NA NA hsa-miR-21-5p MORC3 1.51084295924322 1.54551622173259e-34 -0.493622872657661 7.75189399922391e-06 miRNAWalker2_validate -0.336982344826891 5.83692743621512e-18 NA NA NA hsa-miR-21-5p MPP5 1.51084295924322 1.54551622173259e-34 -0.288077654324143 0.00156212311296768 miRNAWalker2_validate -0.261426709918366 3.95335462458201e-16 NA NA NA hsa-miR-221-3p MRAP 1.12244756358612 3.48370308238012e-13 -1.1227534347457 0.0175857112926596 MirTarget -0.337299951028342 0.0207787687025171 NA NA NA hsa-miR-21-5p MTMR12 1.51084295924322 1.54551622173259e-34 -0.30433091231081 8.46080653212321e-05 miRNAWalker2_validate;miRNATAP -0.153608728333415 3.79045501898405e-08 NA NA NA hsa-miR-21-5p MTMR9 1.51084295924322 1.54551622173259e-34 -0.787590544067709 5.77939416034669e-15 miRNAWalker2_validate -0.204464978203831 4.90417157828363e-08 NA NA NA hsa-miR-21-5p MYCBP2 1.51084295924322 1.54551622173259e-34 -0.593972864681513 6.8369331659891e-07 miRNAWalker2_validate -0.207075485776808 2.20253115650157e-06 NA NA NA hsa-miR-221-3p MYO10 1.12244756358612 3.48370308238012e-13 -1.59578787890497 5.76385780041621e-13 MirTarget;miRNATAP -0.155827611349414 0.0264178140204685 NA NA NA hsa-miR-21-5p MYO9A 1.51084295924322 1.54551622173259e-34 -1.00759496257002 7.50264844143602e-06 miRNAWalker2_validate -0.453185104639855 3.01731319012402e-08 NA NA NA hsa-miR-21-5p N4BP2L1 1.51084295924322 1.54551622173259e-34 -1.71399858767707 1.0517231633165e-27 MirTarget -0.739815199438225 2.03614634263877e-40 NA NA NA hsa-miR-21-5p NAA30 1.51084295924322 1.54551622173259e-34 -0.454004458262681 8.01308724482182e-10 miRNAWalker2_validate;MirTarget -0.225387831025051 1.09431284378073e-17 NA NA NA hsa-miR-221-3p NAP1L5 1.12244756358612 3.48370308238012e-13 -1.29692509941602 3.32250714880114e-12 MirTarget;miRNATAP -0.34607457043993 2.16429669348486e-09 NA NA NA hsa-miR-221-3p NDFIP1 1.12244756358612 3.48370308238012e-13 -0.228407565966697 0.00532073775853203 miRNAWalker2_validate -0.149849589513468 1.33268201682902e-09 NA NA NA hsa-miR-221-3p NDST3 1.12244756358612 3.48370308238012e-13 -5.21438460909548 2.47688530616434e-64 MirTarget -0.506438753862259 4.32396104959882e-06 NA NA NA hsa-miR-21-3p NDUFA5 -0.484219378716006 0.00299941098417659 -0.406708643172571 3.49036349124771e-05 mirMAP -0.126325942117954 1.59278227040421e-05 NA NA NA hsa-miR-221-3p NDUFS1 1.12244756358612 3.48370308238012e-13 -0.676344923057284 6.13862508636795e-12 miRNAWalker2_validate -0.205293687765296 1.14953251431145e-11 NA NA NA hsa-miR-21-5p NEK1 1.51084295924322 1.54551622173259e-34 -0.421013421815401 0.00013611043051042 miRNAWalker2_validate -0.217073502904145 5.42988662846301e-08 NA NA NA hsa-miR-21-3p NEK9 -0.484219378716006 0.00299941098417659 0.0585881900267706 0.4098817465597 MirTarget -0.114820544832342 3.12529861730142e-08 NA NA NA hsa-miR-21-5p NFAT5 1.51084295924322 1.54551622173259e-34 -0.326144498869474 0.00164314559569324 miRNAWalker2_validate;mirMAP -0.110763353790018 0.00341649802767152 NA NA NA hsa-miR-21-5p NFIA 1.51084295924322 1.54551622173259e-34 -0.924685216157224 2.60745966247054e-13 MirTarget;miRNATAP -0.531732418939975 5.46694443575908e-34 NA NA NA hsa-miR-21-5p NFIB 1.51084295924322 1.54551622173259e-34 -0.473125743246742 0.000230272053316789 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.229349055079126 8.74417104722524e-07 NA NA NA hsa-miR-21-5p NFIC 1.51084295924322 1.54551622173259e-34 -0.577436174020039 0.00748919731318304 mirMAP -0.483685235297627 4.40025750800735e-10 NA NA NA hsa-miR-21-5p NHLRC2 1.51084295924322 1.54551622173259e-34 -1.1330149468621 2.05949478884869e-14 mirMAP -0.402866583052703 1.13908305342286e-13 NA NA NA hsa-miR-21-5p NKIRAS1 1.51084295924322 1.54551622173259e-34 -1.03217749329624 2.29315553156374e-18 MirTarget -0.463907525797565 2.32268152012086e-28 NA NA NA hsa-miR-221-3p NRG1 1.12244756358612 3.48370308238012e-13 -3.95953255287574 8.76353628841238e-16 miRNATAP -1.23477474002632 3.94326582819637e-16 NA NA NA hsa-miR-21-5p NTF3 1.51084295924322 1.54551622173259e-34 -3.97962534066229 2.44784627289438e-41 miRTarBase;miRNATAP -1.02982933864977 2.33503442659123e-19 NA NA NA hsa-miR-221-3p NTF3 1.12244756358612 3.48370308238012e-13 -3.97962534066229 2.44784627289438e-41 miRNATAP -0.487132834663555 1.03773554125066e-06 NA NA NA hsa-miR-21-5p NTRK2 1.51084295924322 1.54551622173259e-34 -2.30467079336051 6.66762032408277e-09 mirMAP -0.307805996175828 0.0376038333861936 NA NA NA hsa-miR-21-5p NUBPL 1.51084295924322 1.54551622173259e-34 -0.487181955870277 0.000338824031290841 miRNAWalker2_validate -0.390836294612581 4.75661868182593e-16 NA NA NA hsa-miR-21-5p NUDT16 1.51084295924322 1.54551622173259e-34 -0.306472623503673 0.00173007556301039 mirMAP -0.195353647088521 2.96129833416772e-08 NA NA NA hsa-miR-21-3p ONECUT2 -0.484219378716006 0.00299941098417659 0.120890541524141 0.551384233388767 mirMAP -0.215264361287505 0.000352273373201946 NA NA NA hsa-miR-21-5p OSBPL1A 1.51084295924322 1.54551622173259e-34 -0.743477471816192 2.63416935352607e-06 miRNAWalker2_validate -0.155153775737544 0.0078788472837604 NA NA NA hsa-miR-21-5p OSBPL6 1.51084295924322 1.54551622173259e-34 -0.977274624507167 6.64307851482683e-05 mirMAP -0.344549480682748 0.00012294985070846 NA NA NA hsa-miR-21-5p OTUD1 1.51084295924322 1.54551622173259e-34 -0.719143389849849 2.30062508510608e-14 miRNAWalker2_validate -0.218830236552547 2.98514655251898e-10 NA NA NA hsa-miR-21-5p OTUD4 1.51084295924322 1.54551622173259e-34 -0.781147538845887 2.89406618341624e-12 mirMAP -0.2461519497621 2.22000817829873e-09 NA NA NA hsa-miR-221-3p PAFAH1B2 1.12244756358612 3.48370308238012e-13 -0.49682050496285 0.00261299670984924 MirTarget -0.143740707434348 0.00473106063053187 NA NA NA hsa-miR-21-5p PAIP2B 1.51084295924322 1.54551622173259e-34 -2.26694774889415 1.51639004034747e-12 miRNATAP -0.581637444658434 1.05014180588205e-06 NA NA NA hsa-miR-21-5p PALM2 1.51084295924322 1.54551622173259e-34 -2.07895743328401 4.60304002325711e-20 mirMAP -0.717919756509347 7.43976394867784e-18 NA NA NA hsa-miR-21-5p PANK3 1.51084295924322 1.54551622173259e-34 -0.310601166776069 0.00575629167578977 mirMAP -0.232870955587185 8.54810274373579e-09 NA NA NA hsa-miR-21-3p PARD3B -0.484219378716006 0.00299941098417659 -0.31418892508061 0.257688654850123 MirTarget -0.320829748306835 9.79612981131347e-05 NA NA NA hsa-miR-21-5p PARP9 1.51084295924322 1.54551622173259e-34 -0.616306428128511 2.79656140991419e-08 miRNAWalker2_validate -0.169817598803572 3.2104242462217e-05 NA NA NA hsa-miR-21-5p PBRM1 1.51084295924322 1.54551622173259e-34 -0.300407126251519 0.000299318324625375 miRNAWalker2_validate -0.18266380398239 9.06357874420144e-10 NA NA NA hsa-miR-21-5p PBX1 1.51084295924322 1.54551622173259e-34 -1.3362034667203 5.80856733969378e-07 miRNAWalker2_validate -0.391114448383062 7.03160449429696e-05 NA NA NA hsa-miR-21-5p PCBP2 1.51084295924322 1.54551622173259e-34 -0.236371834912035 0.00019771729259111 MirTarget;miRNATAP -0.110749884572612 1.33904259299185e-06 NA NA NA hsa-miR-221-3p PCDHA12 1.12244756358612 3.48370308238012e-13 -2.68228512919554 1.22349401150652e-09 MirTarget -0.463108560507881 0.000806976964917824 NA NA NA hsa-miR-221-3p PCDHA13 1.12244756358612 3.48370308238012e-13 -2.25664029288571 8.67102156279892e-07 MirTarget -0.504720242506018 0.000395994502377721 NA NA NA hsa-miR-221-3p PCDHAC1 1.12244756358612 3.48370308238012e-13 -2.39947483963875 3.46248760946361e-10 MirTarget -0.479141329586346 6.12131524523156e-05 NA NA NA hsa-miR-221-3p PCDHAC2 1.12244756358612 3.48370308238012e-13 -2.81338526483965 3.55719413756446e-10 MirTarget -0.579687250037446 3.54355659202688e-05 NA NA NA hsa-miR-21-5p PCGF5 1.51084295924322 1.54551622173259e-34 -0.536598424916245 4.26862146890107e-10 mirMAP -0.23487459167029 3.37819673993488e-14 NA NA NA hsa-miR-21-3p PCMTD2 -0.484219378716006 0.00299941098417659 -0.0149749491376436 0.876755700702286 MirTarget -0.17411753126397 6.08555267812655e-10 NA NA NA hsa-miR-21-5p PCSK6 1.51084295924322 1.54551622173259e-34 -1.18950076181489 3.29501368019698e-09 MirTarget;miRNATAP -0.577125974927784 1.46237272255029e-15 NA NA NA hsa-miR-21-5p PDCD4 1.51084295924322 1.54551622173259e-34 -0.0963058046431708 0.345660764291274 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.133707586805023 0.000300820272836491 22322403;23604124;20978511;25973032;25575817;25316501;22072622;22267128;19836969;22212233;25520758;19682430;23272133;25477028;26252635;25994220;25991676;22747440;23827854;23824073;24865582;24902663;24888238;24149370;17991735;22689922;27355932;19308091;24331411;26169933;22547075;20372781;23558936;23201752;20815812;17968323;25310697;21842656;23567619;20581857;23888942;23548551;19013014;21685938;26142886;24472409;26418978;24659669;22086602;26212010;25543482;25543261;23904372;27725205;23564788;25400316;26150475;21546206;19509156;27035745;23052036;22353043;19633292;25566594;21412018;19276261;21330826;26864640;22335905;21636706 miR 21 promotes migration and invasion by the miR 21 PDCD4 AP 1 feedback loop in human hepatocellular carcinoma; The expression level of miR-21 was inversely correlated with the protein expression level of a previously identified target gene programmed cell death 4 PDCD4;HBx mediated miR 21 upregulation represses tumor suppressor function of PDCD4 in hepatocellular carcinoma; HBx downregulates PDCD4 expression at least partially through miR-21; Taken together this study reported for the first time that HBx downregulates PDCD4 and upregulates miR-21 expression; The deregulation of PDCD4 by HBx through miR-21 represents a potential novel mechanism of the downregulation of PDCD4 in HBV-related HCC and provides new insights into HCC development;Transfection of anti-miR-21 rendered HCC cells sensitive to IFN-α/5-FU and such sensitivity was weakened by transfection of siRNAs of target molecules PETN and PDCD4 miR-21 expression in clinical HCC specimens was significantly associated with the clinical response to the IFN-α/5-FU combination therapy and survival rate;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;We identified miR-21 was directly downregulated by RBP2 and found that miR-21 downregulated PDCD4 expression in leukemia cells;MTCs were consistently associated with miR-21 up-regulation P < .0016 and featured significant PDCD4 nuclear down-regulation; An inverse correlation emerged between miR-21 overexpression and PDCD4 down-regulation P = .0013; This study showed in MTCs that miR-21 regulates PDCD4 expression and also that the miR-21/PDCD4 pathway correlates with clinicopathological variables and prognosis;Although microRNA-21 miR-21 is emerging as an oncogene and has been shown to target several tumor suppressor genes including programmed cell death 4 PDCD4 its precise mechanism of action on cancer stem cells CSCs is unclear; Herein we report that FOLFOX-resistant HCT-116 and HT-29 cells that are enriched in CSCs show a 3- to 7-fold upregulation of pre- and mature miR-21 and downregulation of PDCD4; Likewise overexpression of miR-21 in HCT-116 cells achieved through stable transfection led to the downregulation of PDCD4 and transforming growth factor beta receptor 2 TGFβR2;Prognostic significance of PDCD4 expression and association with microRNA 21 in each Dukes' stage of colorectal cancer patients; Furthermore relationships between the PDCD4 mRNA and microRNA-21 miR-21 were evaluated; The PDCD4 mRNA was investigated by the quantitative real-time RT-PCR method and miR-21 was examined by TaqMan microRNA assays; Significant inverse correlations were demonstrated between PDCD4 and miR-21;This short review will focus on our recent finding that the microRNA miR-21 posttranscriptionally regulates Pdcd4 as well as invasion intravasation and metastasis;PDCD4 expression inversely correlated with miR 21 levels in gastric cancers;MicroRNA 21 Down regulates Rb1 Expression by Targeting PDCD4 in Retinoblastoma; MicroRNA-21 miR-21 possesses the oncogenic potential to target several tumor suppressor genes including PDCD4 and regulates tumor progression and metastasis; However the mechanism of how miR-21 regulates PDCD4 is poorly understood in RB; Using the TargetScan program we identified a list of potential target genes of these miRNAs of which PDCD4 is one the targets of miR-21; In this study we focused on the regulatory mechanism of miR-21 on PDCD4 in RB; We demonstrated an inverse correlation between miR-21 and PDCD4 expression in Weri-Rb1 and Y79 cells; These data suggest that miR-21 down-regulates Rb1 by targeting PDCD4 tumor suppressor;MicroRNA 21 promotes cell proliferation and down regulates the expression of programmed cell death 4 PDCD4 in HeLa cervical carcinoma cells; In this study we found that the inhibition of miR-21 in HeLa cervical cancer cells caused profound suppression of cell proliferation and up-regulated the expression of the tumor suppressor gene PDCD4;Additionally resveratrol increased the expression of tumor suppressors PDCD4 and maspin which are negatively regulated by miR-21; Short interfering si RNA against PDCD4 attenuated resveratrol's effect on prostate cancer cells and similar effects were observed following over expression of miR-21 with pre-miR-21 oligonucleotides; MiR-21 expression in these cells appeared to be dependent on Akt as LY294002 reduced the levels of miR-21 along with a concurrent increase in PDCD4 expression; These tumor- and metastatic-suppressive effects of resveratrol were associated with reduced miR-21 and pAkt and elevated PDCD4 levels;The expression of miR-21 and PDCD4 mRNA in transfected cells was quantified by real-time polymerase chain reaction and the expression of PDCD4 protein by Western blot; Cell proliferation apoptosis migration and invasion assays were performed to determine the biological effects of miR-21 expression and PDCD4 inhibition; Co-transfection of miR-21-sponge with PDCD4 siRNA upregulated miR-21 expression in these cells; PDCD4 mRNA and protein levels were increased 2.14-fold and 2.16-fold respectively following inhibition of miR-21 expression; Depletion of PDCD4 by siRNA transfection reversed the reduction of cell proliferation migration and invasion induced by inhibition of miR-21 in A549 cells; It indicates that miR-21 is highly expressed in patients with NSCLC and inhibition of miR-21 expression reduces proliferation migration and invasion of A549 cells by upregulating PDCD4 expression; Modulation of miR-21 or PDCD4 expression may provide a potentially novel therapeutic approach for NSCLC;IL 6 Inhibits the Targeted Modulation of PDCD4 by miR 21 in Prostate Cancer; In this study we evaluated the role of IL-6 in PDCD4 gene expression and how the microRNA miR-21 regulates this process in prostate cancer cell lines PC-3 and LNCaP; The expression of PDCD4 was inhibited by IL-6 but this effect was weakened in cell lines with low expression of miR-21; These findings support the feasibility of future efforts for diagnosis and gene therapy for prostate cancer that are based on IL-6 miR-21 and PDCD4;Furthermore the absence of miR-21 increased the expression of its target gene PDCD4 and subsequently modulated nuclear factor NF-κB activation;In this study using reverse phase protein arrays RPPAs we assessed MPS1 mediated cell signaling pathways and demonstrated that inhibiting MPS1 could upregulate the expression of the tumor suppressor PDCD4 and MSH2 genes by down regulating micro RNA-21 miR-21; We also show the prognostic effect of miR21 PDCD4 and MSH2 levels to patient survival across different GBM molecular subtypes;We also analyzed the immunohistochemical expression of PDCD4 an miR-21 gene target; PDCD4 expression was significantly downregulated in MTC samples consistent with miR-21 upregulation;Aldose reductase inhibition suppresses colon cancer cell viability by modulating microRNA 21 mediated programmed cell death 4 PDCD4 expression; Further AR inhibition also increased PDCD4 a putative target of miR-21 in human colon cancer cells; Collectively these results indicate that AR inhibition prevents growth factor-induced colon cancer growth by down-regulating miR-21 expression and increasing PDCD4 levels through the reactive oxygen species ROS/AMPK/mTOR/AP1/4E-BP1 pathway;Berberine could inhibit miR-21 expression and function in ovarian cancer as shown by an enhancement of its target PDCD4 an important tumor suppressor in ovarian cancer;Overexpression of miR-21 in cisplatin sensitive cells decreased PDCD4 levels and increased cell proliferation;MicroRNA 21 miR 21 post transcriptionally downregulates tumor suppressor PDCD4 and promotes cell transformation proliferation and metastasis in renal cell carcinoma; MiR-21 induces neoplastic transformation cell proliferation and metastasis and downregulates programmed cell death4 PDCD4 in some cancers; The aim of this study was to investigate the roles and interactions of PDCD4 and miR-21 in human renal cell carcinoma RCC; The expression levels of PDCD4 protein and mRNA and miR-21 were examined by Western blot analysis and by qRT-PCR and luciferase reporter assays; MiR-21 not only promoted cancer cell hyperplasia and contributed to tumor cell transformation and metastasis but also post-transcriptionally downregulated PDCD4 protein expression; PDCD4 and miR-21 expression levels potentially play an important role in renal cell cancer;Programmed cell death 4 PDCD4 is a tumor suppressor gene whose expression is directly controlled by microRNA-21 miR-21; This PDCD4 and miR-21 inverse expression was also noted in cells and exosomes from OSC peritoneal effusions compared with nonneoplastic effusions; PDCD4 and miR-21 are involved in OSC oncogenesis;Finally in a subgroup of tumor specimens ROC curve analysis performed on quantitative PCR data sets for miR-21 ITGβ4 and PDCD4 shows that the combination of high miR-21 with low ITGβ4 and PDCD4 expression is able to predict presence of metastasis; In conclusion miR-21 is a key player in oncogenic EMT its overexpression is controlled by the cooperation of genetic and epigenetic alterations and its levels along with ITGβ4 and PDCD4 expression could be exploited as a prognostic tool for CRC metastasis;Programmed cell death 4 PDCD4 is an important functional target of the microRNA miR 21 in breast cancer cells;In ZD esophagus and tongue oncogenic miR-31 and miR-21 overexpression was accompanied by down-regulation of their respective tumor-suppressor targets PPP2R2A and PDCD4;Due to inhibition of miR-21 expression of the programed cell death 4 PDCD4 gene was promoted in tumors resulting in the induction of apoptosis of tumor cells;We also demonstrated that BMP-6-induced downregulation of miR-21 modified the activity of PDCD4 3'UTR and inhibited MDA-MB-231 cell invasion;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;The results indicated that the miR-21 inhibition could induce the cell cycle arrest in G1 phase upregulate the expression of Programmed Cell Death Protein 4 PDCD4 and thus active the caspase-3 apoptosis pathway;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;Clinicopathological and prognostic significance of PDCD4 and microRNA 21 in human gastric cancer; Recent studies have demonstrated that the novel tumor suppressor protein programmed cell death 4 PDCD4 is downregulated in several human solid cancer types and is suppressed by microRNA-21 miR-21; The objectives of this study were: i to establish the clinicopathological and prognostic significance of PDCD4 mRNA and ii to elucidate any correlation between PDCD4 mRNA and miR-21 in gastric cancer; We also performed an association study comparing PDCD4 mRNA and miR-21 in eight cell lines and 49 gastric cancers; An inverse correlation between PDCD4 mRNA and miR-21 was found in gastric cancer; Furthermore our findings suggest that PDCD4 mRNA is negatively regulated by miR-21 in gastric cancer and may serve as a target for effective therapies;Furthermore miR-21 mimic or inhibitor significantly reduced or increased the expression of PDCD4 and TPM1; MiR-21 is overexpressed in RCC tissue and modulates the growth apoptosis and cell cycle progression of RCC cells and regulates the expression of PDCD4 and TPM1;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;Curcumin treatment reduced miR-21 promoter activity and expression in a dose-dependent manner by inhibiting AP-1 binding to the promoter and induced the expression of the tumour suppressor Pdcd4 programmed cell death protein 4 which is a target of miR-21; Taken together this is the first paper to show that curcumin inhibits the transcriptional regulation of miR-21 via AP-1 suppresses cell proliferation tumour growth invasion and in vivo metastasis and stabilizes the expression of the tumour suppressor Pdcd4 in colorectal cancer;MicroRNA 21 miR 21 post transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion intravasation and metastasis in colorectal cancer; This is the first study to show that Pdcd4 is negatively regulated by miR-21;Inflammation and MiR 21 pathways functionally interact to downregulate PDCD4 in colorectal cancer; These findings indicate that miR-21 is a component of the COX-2 inflammation pathway and that this pathway promotes worsening of disease stage in colorectal cancer by inducing accumulation of PGE2 and increasing expression of miR-21 with consequent downregulation of the tumour suppressor gene PDCD4;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Interestingly NVP-LDE-225 induced PDCD4 and apoptosis and inhibited cell viability by suppressing miR-21;PDCD4 a direct target gene of miR-21 could mediate chemoresistance to docetaxel in PC3 cells;Oxidative stress upregulates PDCD4 expression in patients with gastric cancer via miR 21; The expression of miR-21 and PDCD4 was highly correlated with the degree of differentiation tumor staging local lymphatic node metastasis and remote metastasis; In addition the relative expression of PDCD4 was negatively correlated with miR-21; Thus we conclude that ROS promotes gastric carcinogenesis via upregulating miR-21 expression which in turn down-regulates the expression of PDCD4 in gastric cancer cells;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;MicroRNA 21 down regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G; Expression of PDCD4 protein correlates inversely with expression of miR-21 in a number of human glioblastoma cell lines such as T98G A172 U87 and U251; Inhibition of miR-21 increases endogenous levels of PDCD4 in cell line T98G and over-expression miR-21 inhibits PDCD4-dependent apoptosis;Treatment of HSC-3 cells with Nanog- and/or Stat-3-specific small interfering RNAs effectively blocks HA-mediated Nanog-Stat-3 signaling events abrogates miR-21 production and increases PDCD4 expression;Programmed cell death 4 PDCD4 is critical in mediating apoptosis in GBM and is downregulated by miR-21 which may mediate the resistance of glioblastoma cells against chemotherapy or radiation via its target genes PDCD4; This review will focus on the roles of miRNAs family members particularly miR-21 and its target gene PDCD4 in tumors like glioblastoma and new targeting strategies as examples some new targeting therapeutic methods and molecular mechanisms of signal pathways in glioblastoma therapeutics to give the reader the current trends of approach to target regulation of these miRNA and genes for future glioma therapies;Using Western blot analysis miR-21 knockdown enhanced the expression of tumor suppressor PDCD4 and attenuated apoptosis inhibitor c-IAP2;METHODS 3-45-dimethylthiazol-2-yl-25-diphenyltetrazolium bromide MTT assay was used to determine the effect of 5-FU on the viability of RKO cells with knockout of miR-21 or high expression of PDCD4;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;The effects were accompanied by up-regulation of tumor suppressor PDCD4 a known miR-21 target; as well as up-regulation of cofilin and profilin two key proteins involved in actin polymerization and cytoskeleton maintenance as a consequence of miR-182 down-regulation;Increased levels of miR-21 in turn downregulate the expression of the metastasis-suppressor protein programmed cell death 4 PDCD4 a validated miR-21 target;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;In this study we analyzed the expression of miR-21 and of its target PDCD4 Programmed Cell Death 4 during normal hematopoietic differentiation and in AMLs;MiR 21 mediates the radiation resistance of glioblastoma cells by regulating PDCD4 and hMSH2; In addition miR-21 knockdown increased the expression of endogenous PDCD4 and hMSH2 which contributed to the apoptosis and G2 arrest of T98G cells; The findings suggested that miR-21 may mediate the resistance of glioblastoma cells against radiation via its target genes PDCD4 and hMSH2;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated;The expression of programmed cell death-4 PDCD4 was increased by I3C and reduced by miR-21 transfection; Such enhanced chemosensitivity might be explained by the increased expression of PDCD4 which is a downstream target which miR-21 negatively regulates;It has been reported that miR-21 was upregulated in most malignant cancers the proposed mechanism of which was through suppressing expression of programmed cell death 4 PDCD4; In present study it is firstly reported that miR-21 was upregulated in Kazakh's ESCC and that miR-21 played a negative role in regulating PDCD4 using in situ hybridization ISH and luciferase reporter approach;To measure levels of microRNA miR-21 and its target gene programmed cell death 4 PDCD4 in samples of human cutaneous malignant melanoma and normal non-malignant control skin; Relative levels of miR-21 and PDCD4 mRNA were measured using a quantitative real-time reverse transcription-polymerase chain reaction; Compared with normal skin samples the relative level of miR-21 was significantly higher and the relative level of PDCD4 mRNA was significantly lower in the melanoma specimens; A significant negative correlation between PDCD4 mRNA and miR-21 was demonstrated in malignant melanoma r = -0.602; Elevated miR-21 and reduced PDCD4 mRNA levels were both significantly correlated with increased tumour size a higher Clark classification level and the presence of lymph node metastases in malignant melanoma; These findings suggest that miR-21 and PDCD4 might be potential biomarkers for malignant melanoma and might provide treatment targets in the future;MicroRNA 21 and PDCD4 expression in colorectal cancer; Studies have shown that miR-21 exerts its oncogenic activity by targeting the PDCD4 tumour suppressor 3'-UTR; The purpose of this study was to delineate the interaction between PDCD4 and its oncogenic modulator miR-21 in colorectal cancer; A cohort of 48 colorectal tumours 61 normal tissues and 7 polyps were profiled for miR-21 and PDCD4 gene expression; A significant inverse relationship between miR-21 and PDCD4 gene expression p < 0.001 was identified by RT-qPCR; In addition significant reduction of PDCD4 p < 0.001 expression and reciprocal upregulation of miR-21 p = 0.005 in a progressive manner from tumour-polyp-normal mucosae was identified; This study demonstrates the inverse relationship between miR-21 and PDCD4 thus suggesting that miR-21 post-transcriptionally modulates PDCD4 via mRNA degradation;Expression patterns of miR-21 RNA and its target tumor suppressor protein PDCD4 were mutually exclusive;Exosome shuttling microRNA 21 promotes cell migration and invasion targeting PDCD4 in esophageal cancer;Fenhexamid and fludioxonil stimulated miR-21 expression in a concentration-dependent manner and reduced the expression of miR-21 target Pdcd4 protein; Antisense to miR-21 blocked the increase in Pdcd4 protein by fenhexamid and fludioxonil;The expression of programmed cell death 4 PDCD4 which is a miR-21 targeting apoptotic gene has also been enhanced by UA; And over-expression of miR-21 in U251 cells abolished the enhancement of PDCD4 protein by UA;This process leads to microRNA-21 miR-21 production and a tumor suppressor protein e.g PDCD4 program cell death 4 reduction; Transfection of MCF-7 cells with PKCepsilon or Nanog-specific small interfering RNAs effectively blocks HA-mediated PKCepsilon-Nanog signaling events abrogates miR-21 production and increases PDCD4 expression/eIF4A binding;Through dual-luciferase method it was verified that PDCD4 and PDCD10 were real target genes of miR-21 and miR-200c respectively;miR-21 and miR-155 expression was assessed in tumor tissue and in adjacent normal tissue of 156 CRC patients by TaqMan MicroRNA assays and PDCD4 and TP53INP1 mRNA levels were measured by quantitative real-time reverse transcriptase PCR RT-PCR; Significant inverse correlations were demonstrated between miR-21 and PDCD4 mRNA and miR-155 and TP53INP1 mRNA;The aim of this study was a to determine a role of microRNA-21 in esophageal squamous cell carcinoma and b to elucidate the regulation of the programmed cell death 4 PDCD4 gene by microRNA-21; In addition the regulation of PDCD4 by microRNA-21 was elucidated to identify the mechanisms of this regulation; The PDCD4 protein levels in esophageal squamous cell carcinoma cells have an inverse correlation with microRNA-21 expression; MicroRNA-21 targets PDCD4 at the posttranscriptional level and regulates cell proliferation and invasion in esophageal squamous cell carcinoma;Maspin Pdcd4 and miR-21 expressions were evaluated by a real-time polymerase chain reaction in 20 endometrial cancer and 10 normal endometrium samples; Comparison between IA and more advanced International Federation of Gynecology and Obstetrics stages of endometrial cancer in regard to expression levels of maspin Pdcd4 and miR-21 did not reveal any significant differences;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;Sequence prediction and gene expression regulation assays showed that miR-21 could reversely regulate the expression of PDCD4 P < 0.01; Suppression of miR-21 expression is associated with an elevation of Pdcd4 resulting in a significant reduction of proliferation and the apoptosis rate 2.6% was increased to 10.9%; Moreover the anti-proliferation and pro-apoptotic effect by miR-21 suppression could be reversed by PDCD4 knock down;Downregulation of Pdcd4 by mir 21 facilitates glioblastoma proliferation in vivo; We demonstrate that downregulation of mir-21 in glioblastoma-derived cell lines results in increased expression of its target programmed cell death 4 Pdcd4 a known tumor-suppressor gene; In addition our data indicate that either downregulation of mir-21 or overexpression of its target Pdcd4 in glioblastoma-derived cell lines leads to decreased proliferation increased apoptosis and decreased colony formation in soft agar; Using a glioblastoma xenograft model in immune-deficient nude mice we observe that glioblastoma-derived cell lines in which mir-21 levels are downregulated or Pdcd4 is over-expressed exhibit decreased tumor formation and growth; These critical in vivo findings demonstrate an important functional linkage between mir-21 and Pdcd4 and further elucidate the molecular mechanisms by which the known high level of mir-21 expression in glioblastoma can attribute to tumorigenesis--namely inhibition of Pdcd4 and its tumor-suppressive functions ;;poor survival;drug resistance;;;worse prognosis;;staging;metastasis;;metastasis;progression;;;;;;poor survival;;;;;metastasis;;metastasis;;;;;drug resistance;;;;progression;;metastasis;metastasis;staging;;;drug resistance;staging;metastasis;differentiation;tumorigenesis;;;;drug resistance;;;;;metastasis;;differentiation;drug resistance;;;malignant trasformation;malignant trasformation;metastasis;progression;;cell migration;;;;;;;staging;drug resistance;;tumorigenesis liver cancer;liver cancer;liver cancer;liver cancer;chronic myeloid leukemia;thyroid cancer;colon cancer;colorectal cancer;colon cancer;gastric cancer;retinoblastoma;cervical and endocervical cancer;prostate cancer;lung squamous cell cancer;prostate cancer;colorectal cancer;glioblastoma;thyroid cancer;colon cancer;ovarian cancer;ovarian cancer;kidney renal cell cancer;ovarian cancer;colorectal cancer;breast cancer;esophageal cancer;glioblastoma;breast cancer;lung squamous cell cancer;breast cancer;breast cancer;gastric cancer;kidney renal cell cancer;glioblastoma;colorectal cancer;colorectal cancer;colorectal cancer;lung cancer;prostate cancer;prostate cancer;gastric cancer;B cell lymphoma;glioblastoma;head and neck cancer;glioblastoma;ovarian cancer;colon cancer;gastric cancer;breast cancer;breast cancer;B cell lymphoma;acute myeloid leukemia;glioblastoma;prostate cancer;pancreatic cancer;esophageal cancer;melanoma;colorectal cancer;colorectal cancer;esophageal cancer;breast cancer;glioblastoma;breast cancer;breast cancer;colorectal cancer;esophageal cancer;endometrial cancer;pancreatic cancer;lung squamous cell cancer;glioblastoma hsa-miR-21-3p PDIK1L -0.484219378716006 0.00299941098417659 -0.0468630905538001 0.608073460423057 MirTarget -0.133922732787134 5.99762456245728e-07 NA NA NA hsa-miR-221-3p PDIK1L 1.12244756358612 3.48370308238012e-13 -0.0468630905538001 0.608073460423057 miRNAWalker2_validate -0.105968800033143 0.000140077432892211 NA NA NA hsa-miR-21-3p PDP2 -0.484219378716006 0.00299941098417659 -0.449946555806165 0.00178954078552096 MirTarget -0.16756986291705 9.39240662511639e-05 NA NA NA hsa-miR-21-5p PER2 1.51084295924322 1.54551622173259e-34 -0.671467371709996 2.18508455717704e-08 MirTarget -0.31016960095489 7.92967821787459e-13 NA NA NA hsa-miR-21-5p PER3 1.51084295924322 1.54551622173259e-34 -0.848602701375337 1.12316239584011e-06 miRNAWalker2_validate -0.495600144148107 1.73737178564388e-15 NA NA NA hsa-miR-221-3p PEX1 1.12244756358612 3.48370308238012e-13 -0.293374407449564 0.0109875123548264 miRNAWalker2_validate -0.14198576420168 5.72115861711408e-05 NA NA NA hsa-miR-221-3p PEX19 1.12244756358612 3.48370308238012e-13 -0.223033990954226 0.0244750913396949 miRNAWalker2_validate -0.117045200308747 0.000111562529685485 NA NA NA hsa-miR-21-3p PGPEP1 -0.484219378716006 0.00299941098417659 0.167322965185669 0.143077647806586 mirMAP -0.117663327232917 0.000518658494580287 NA NA NA hsa-miR-21-5p PGRMC2 1.51084295924322 1.54551622173259e-34 -0.34369815717862 7.20024903580005e-05 miRNAWalker2_validate -0.181193895693892 6.43859631847864e-09 NA NA NA hsa-miR-21-5p PHACTR2 1.51084295924322 1.54551622173259e-34 -0.74980974150153 3.84085236018818e-07 miRNAWalker2_validate -0.139329194785773 0.0107368595820114 NA NA NA hsa-miR-21-3p PHF2 -0.484219378716006 0.00299941098417659 -0.285706277302981 0.00535336932565434 MirTarget -0.123784822313365 4.76439596413739e-05 NA NA NA hsa-miR-21-3p PHYHIPL -0.484219378716006 0.00299941098417659 0.875425421453594 0.00299276786748856 MirTarget -0.244772558484879 0.00556496793769685 NA NA NA hsa-miR-21-5p PIGN 1.51084295924322 1.54551622173259e-34 -0.115796664312666 0.17921793826658 miRNAWalker2_validate -0.134350451621118 1.5560087613616e-05 NA NA NA hsa-miR-21-5p PIK3C2A 1.51084295924322 1.54551622173259e-34 -0.190979416238561 0.0562063266883107 miRNAWalker2_validate -0.178042173346167 7.80247659980854e-07 NA NA NA hsa-miR-21-5p PIK3R1 1.51084295924322 1.54551622173259e-34 -0.891953424131491 1.38465728969785e-08 miRNAWalker2_validate;MirTarget;miRNATAP -0.626634426633867 6.01244523464713e-31 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-221-3p PIK3R1 1.12244756358612 3.48370308238012e-13 -0.891953424131491 1.38465728969785e-08 MirTarget -0.217914846238424 7.87055620620445e-06 NA NA NA hsa-miR-21-5p PIKFYVE 1.51084295924322 1.54551622173259e-34 -0.360642566218591 0.00157442514854895 MirTarget -0.251222573601493 8.81825641630044e-10 NA NA NA hsa-miR-21-3p PITPNM2 -0.484219378716006 0.00299941098417659 0.252057635121367 0.0799674757736623 mirMAP -0.18373445328703 1.62121647287956e-05 NA NA NA hsa-miR-221-3p PITPNM2 1.12244756358612 3.48370308238012e-13 0.252057635121367 0.0799674757736623 MirTarget -0.120283319820245 0.00652412301490194 NA NA NA hsa-miR-21-5p PJA2 1.51084295924322 1.54551622173259e-34 -0.547154205949659 1.53194178694233e-08 MirTarget -0.246478997567002 1.64079176744364e-12 NA NA NA hsa-miR-21-5p PLEKHA1 1.51084295924322 1.54551622173259e-34 -0.551113202524991 2.34631688636684e-09 miRNAWalker2_validate;MirTarget;miRNATAP -0.18932877131577 1.98834273745768e-08 NA NA NA hsa-miR-221-3p PLSCR4 1.12244756358612 3.48370308238012e-13 -2.21840265832849 1.26161246830308e-31 MirTarget -0.415370900712108 1.53717954104031e-11 NA NA NA hsa-miR-21-5p POLR3B 1.51084295924322 1.54551622173259e-34 -0.413501114011505 1.11276506966565e-07 miRNAWalker2_validate -0.188714653183677 1.78932901569377e-11 NA NA NA hsa-miR-21-3p PPARA -0.484219378716006 0.00299941098417659 -0.607171871846345 3.30592576448713e-05 mirMAP -0.254611975630506 3.93539631619617e-09 22244963 In the final integrative analysis of lung cancer related miR-21-targets analysis 24 hub genes were identified by overlap calculation suggesting that miR-21 may play an important role in the development and progression of lung cancer through JAK/STAT signal pathway MAPK signaling pathway Wnt signaling pathway cell cycle PPAR signaling pathway apoptosis pathway and other pathways progression lung cancer hsa-miR-21-5p PPARA 1.51084295924322 1.54551622173259e-34 -0.607171871846345 3.30592576448713e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.393331541274169 5.04548601402031e-14 22244963 In the final integrative analysis of lung cancer related miR-21-targets analysis 24 hub genes were identified by overlap calculation suggesting that miR-21 may play an important role in the development and progression of lung cancer through JAK/STAT signal pathway MAPK signaling pathway Wnt signaling pathway cell cycle PPAR signaling pathway apoptosis pathway and other pathways progression lung cancer hsa-miR-221-3p PPARGC1A 1.12244756358612 3.48370308238012e-13 -1.91181447721978 1.53762022606371e-08 miRNATAP -0.865804752132837 1.77855991900398e-17 NA NA NA hsa-miR-21-5p PPIF 1.51084295924322 1.54551622173259e-34 -0.769387045826857 5.54110086115503e-11 miRNAWalker2_validate -0.367561626641704 2.21090335244967e-18 NA NA NA hsa-miR-21-3p PPM1A -0.484219378716006 0.00299941098417659 -0.491863739444724 3.59260984655925e-09 MirTarget -0.14757126985842 2.69145680315522e-09 NA NA NA hsa-miR-21-5p PPM1L 1.51084295924322 1.54551622173259e-34 -0.358173274286561 0.0774099414261059 miRNAWalker2_validate -0.34957959654957 1.87441685478428e-06 NA NA NA hsa-miR-221-3p PPM1L 1.12244756358612 3.48370308238012e-13 -0.358173274286561 0.0774099414261059 mirMAP -0.347809048765759 1.51226412791389e-08 NA NA NA hsa-miR-21-3p PPP1R1A -0.484219378716006 0.00299941098417659 -2.43709881713158 3.94097460426434e-08 MirTarget -0.289706914850715 0.0316072350970944 NA NA NA hsa-miR-21-5p PPP1R3B 1.51084295924322 1.54551622173259e-34 -1.91981385054872 2.06614046154102e-21 MirTarget;miRNATAP -0.814270913907123 2.33116286083925e-29 NA NA NA hsa-miR-21-5p PPP3CA 1.51084295924322 1.54551622173259e-34 -0.536165287387848 2.77787023947662e-11 miRNATAP -0.143821297395015 1.2695170248385e-06 NA NA NA hsa-miR-221-3p PRDM2 1.12244756358612 3.48370308238012e-13 -0.339899736847659 4.69085239503072e-05 miRNATAP -0.12549511481209 8.66247217462794e-07 NA NA NA hsa-miR-21-5p PREPL 1.51084295924322 1.54551622173259e-34 -0.0505743779794514 0.543479808946717 miRNAWalker2_validate -0.106206266700281 0.000417724264158222 NA NA NA hsa-miR-21-5p PRICKLE2 1.51084295924322 1.54551622173259e-34 -1.36375835552812 1.67599789165395e-08 miRNAWalker2_validate;MirTarget -0.234850115033432 0.00889222361721155 NA NA NA hsa-miR-21-5p PRKAB2 1.51084295924322 1.54551622173259e-34 0.56659891692032 3.41527340619955e-05 miRNAWalker2_validate -0.138674962156893 0.00579019389615852 NA NA NA hsa-miR-21-5p PRKAR2A 1.51084295924322 1.54551622173259e-34 -0.445956894636509 0.039701655854281 mirMAP -0.324627317167112 3.73345274692546e-05 NA NA NA hsa-miR-21-5p PRKCE 1.51084295924322 1.54551622173259e-34 -0.525415812633483 0.00121438245155814 miRNAWalker2_validate -0.368254580492906 2.77693046693172e-10 NA NA NA hsa-miR-21-5p PRKG1 1.51084295924322 1.54551622173259e-34 -1.38853534989461 1.21422425786253e-07 mirMAP -0.4069850497267 2.52993522002991e-05 NA NA NA hsa-miR-21-5p PRRG1 1.51084295924322 1.54551622173259e-34 -0.908192707084856 3.41333706973093e-12 MirTarget -0.29970624846694 4.07127207882099e-10 NA NA NA hsa-miR-21-5p PTAR1 1.51084295924322 1.54551622173259e-34 -0.30295618795535 0.00286391397269786 miRNAWalker2_validate;mirMAP -0.257873578273132 9.8848770375557e-13 NA NA NA hsa-miR-21-5p PTCH1 1.51084295924322 1.54551622173259e-34 -0.77814763515361 7.5867355509332e-05 mirMAP -0.229498420855219 0.00147432260745022 NA NA NA hsa-miR-21-5p PTEN 1.51084295924322 1.54551622173259e-34 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;mirMAP -0.235206406510716 3.9272579156044e-11 23684551;17681183;25973032;26311740;26387181;22267008;26384051;22322462;27644439;21471222;21468550;20113523;26905520;21806946;24154840;27611950;24780321;25027758;23036707;26559642;26731559;26847601;27350731;24331411;21820606;25909227;24930006;24293118;26289851;22547075;26666820;24324076;23201752;25963606;27188433;22958183;22956424;19730150;21842656;21104017;23894315;23548551;26236156;23951172;24460329;20092645;25563770;24659669;25647415;26230405;25543482;20048743;27725205;23466500;22922228;25058005;20223231;27220494;22678116;24763002;24221338;22120473;21408027;23174819;22832383;19212625;22978663;25799148;26741162;23226804;26787105;26864640;25603978;26975392 MicroRNA 21 suppresses PTEN and hSulf 1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways;MicroRNA 21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer; PTEN was shown to be a direct target of miR-21 and to contribute to miR-21 effects on cell invasion; Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9 both downstream mediators of PTEN involved in cell migration and invasion; Aberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth migration and invasion;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;Exposure of HCC cells to sorafenib led to an increase in miR-21 expression a decrease in PTEN expression and sequential Akt activation;In addition treated with 5-AZA resulted in significant increases of miR-21 expression in both MCF-7 and MDA-MB-231 cells P < 0.01 with the protein level of PTEN increased in MCF-7 cell which was further involved in the downregulation of AKT;microRNA 21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN; Thus in this study we focused on the expression and significance of miR-21 in gastric cancer tissues and the role of miR-21 in the biological behaviour and the expression of PTEN in gastric cancer cells; Western blotting and the Luciferase Reporter Assay were used to evaluate the change of PTEN expression after lowered expression of miR-21 in gastric cancer cell lines; The western blot results and Luciferase Reporter Assay demonstrated that PTEN expression was remarkably increased after miR-21 inhibition P<0.05 microRNA-21 expression was upregulated in gastric carcinoma tissues and was significantly associated with the degree of differentiation of tumour tissues local invasion and lymph node metastasis;Furthermore we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog PTEN the result of which can be rescued by miR-21 inhibitor;Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon;Bmi-1 also regulates p53 and PTEN via miR-21;Here we demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474 SKBR3 and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody whereas protein expression of the PTEN gene a miR-21 target was reduced; Rescuing PTEN expression with a p3XFLAG-PTEN-mut construct with deleted miR-21 targeting sequence at its 3' UTR restored the growth inhibition of trastuzumab in the resistant cells by inducing PTEN activation and AKT inhibition; In vivo administering miR-21 antisense oligonucleotides restored trastuzumab sensitivity in the resistant breast cancer xenografts by inducing PTEN expression whereas injection of miR-21 mimics conferred trastuzumab resistant in the sensitive breast tumors via PTEN silence;The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting respectively in tumor tissues as well as adjacent non-tumor mucosa; miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues; A negative correlation between expression of miR-21 and PTEN was established in vivo;MicroRNA 21 inhibitor sensitizes human glioblastoma cells U251 PTEN mutant and LN229 PTEN wild type to taxol; Human glioblastoma U251 PTEN-mutant and LN229 PTEN wild-type cells were treated with taxol and the miR-21 inhibitor in a poly amidoamine PAMAM dendrimer alone or in combination; Interestingly the above data suggested that in both the PTEN mutant and the wild-type GBM cells miR-21 blockage increased the chemosensitivity to taxol; Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;The effect of ZA on miR-21 expression was quantified by qRT-PCR and the amount of PTEN protein and its targets were analyzed by Western blot;NTR1 activation stimulates expression of miR-21 and miR-155 in colonocytes via Akt and NF-κB to down-regulate PTEN and SOCS1 and promote growth of tumors in mice;Correlations between the expression levels of miR-21 PTEN and p-AKT were analyzed by real-time PCR and Western blot test in HER2-positive GC cell lines; Overexpression of miR-21 down-regulated PTEN expression increased AKT phosphorylation and did not affect HER2 expression; Inversely suppression of miR-21 increased PTEN expression and down-regulated AKT phosphorylation but still did not affect HER2 expression;MicroRNA 21 promotes TGF β1 induced epithelial mesenchymal transition in gastric cancer through up regulating PTEN expression; In GC tissues the expressions of miR-21 Akt and p-Akt were up-regulated while PTEN expression was down-regulated; These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression;Expressions of PTEN were significantly down-regulated in CRC tissues and negatively correlated with expressions of Notch-1 r2=0.5207 p<0.01 and miR-21 r2=0.6996 p<0.01; These data indicate that the crosstalk between Notch-1 and miR-21 is involved in CRC development through degradation of PTEN;In the same patients we also compared miR-21 expression with the expression of its presumed target PTEN; miR-21 expression levels were found to significantly correlate with tumour size r = 0.403 p = 0.009; Spearman's rank whereas no relation was found between miR-21 and PTEN expression levels Kruskal-Wallis test;Over-expression of miR-21 suppressed its target PTEN and disrupted acinar morphogenesis;There was overexpression of the miR-21 target genes PTEN by 67% and caspase-3 by 15% upon cotreatment;We previously reported that microRNA-21 miR-21 was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target PTEN; Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN stem-loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status; miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression; These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus;Triptolide reduces proliferation and enhances apoptosis of human non small cell lung cancer cells through PTEN by targeting miR 21; To the best of our knowledge the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21;Quantitative real-time PCR qRT-PCR and Western blot were used to detect the expression levels of miR-21 and PTEN in HCT116 HT29 Colo32 and SW480 CRC cell lines; Also the expression levels of PTEN mRNA and its downstream proteins AKT and PI3K in HCT116 cells after downregulating miR-21 were investigated; In comparing the levels of PTEN protein and downstream AKT and PI3K in HCT116 cells after downregulation of miR-21 expression the levels of AKT and PI3K protein expression significantly decreased P < 0.05; PTEN is one of the direct target genes of miR-21;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;MicroRNA 21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN; TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-21 and tumor suppressor gene PTEN protein; We showed that upregulation of miR-21 in MCF-7/ADR cells was concurrent with downregulation of PTEN protein; Overexpression of PTEN could mimic the same effects of miR-21 inhibitor in MCF-7/ADR cells and PTEN-siRNA could increase the resistance of MCF-7 cells to ADR; MiR-21 inhibitor could increase PTEN protein expression and the luciferase activity of a PTEN 3' untranslated region-based reporter construct in MCF-7/ADR cells; Dysregulation of miR-21 plays critical roles in the ADR resistance of breast cancer at least in part via targeting PTEN;MiR-21 level was inversely correlated with the levels of FOXO1 and PTEN in DLBCL cell lines; MiR-21 also down-regulated PTEN expression and consequently activated the PI3K/AKT/mTOR pathway which further decreased FOXO1 expression;In present study we determined the miR-21 levels in TNBC specimens and TNBC cell levels in vitro and then identified the role of miR-21 on tumor cell proliferation apoptosis and then identified PTEN as the possible target of the microRNA;MiR 21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non small cell lung cancer A549 cells; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA was performed to modulate the expression of microRNA-21 and PTEN under the treatment of curcumin; Moreover the protein level of PTEN a putative target of microRNA-21 was significantly elevated in curcumin-treated A549 cells as determined by Western blot analysis; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA significantly P < 0.05 reversed the growth suppression and apoptosis induction by curcumin compared to corresponding controls; Our data suggest a novel molecular mechanism in which inhibition of microRNA-21 and upregulation of PTEN mediate the anticancer activities of curcumin in NSCLC cells;PTEN gene expression was performed as a known target of miR-21;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;PTEN a direct target gene of miR-21 was significantly downregulated in gemcitabine-resistant breast cancer cells and restoration of PTEN expression blocked miR-21-induced EMT and gemcitabine resistance;We also found that Rawq01 up-regulated the expression of PTEN through mir-21 inhibition and therefore inhibited the PI3K-AKT pathway;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;Overexpression of miR 21 promotes the proliferation and migration of cervical cancer cells via the inhibition of PTEN; The aim of this study was to examine the expression of miR-21 and PTEN in cervical cancer specimens using quantitative PCR; miR-21 was upregulated in the cervical cancer specimens negatively correlating with the PTEN mRNA level; Transfection of the miR-21 mimics was markedly promoted whereas the miR-21 inhibitor suppressed the proliferation migration and invasion of cervical cancer cells with a significant inhibition of PTEN expression; The present study showed the upregulation of miR-21 in invasive cervical cancers and confirmed the promotion of miR-21 with regard to the proliferation migration and invasion in cervical cancer cells via inhibiting the PTEN expression;The expressions of miR-21 PTEN PI3K and AKT were detected in 89 esophageal cancer samples and 58 adjacent normal tissues respectively; MiR-21 PI3K and AKT have higher expressions but PTEN has lower expression in esophageal cancer tissues compared with adjacent normal tissues; Further PTEN was a target gene of miR-21;The expression level of miR-21 and PTEN messenger RNA were measured by quantitative real-time reverse transcription polymerase chain reaction or reverse transcription polymerase chain reaction; miR-21 was overexpressed and PTEN was suppressed in established radioresistant TE-R60 cells compared with the parent cells 1.3-fold and 70.83%; The inhibition of miR-21 significantly increased the cells' radiosensitivity P < 0.05 and the PTEN protein expression 2.3-fold in TE-1 cells; Knockdown of PTEN in anti-miR-21 TE-1 cells could abrogate the miR-21 inhibition-induced radiosensitization P < 0.05; Inhibition of miR-21 increased radiosensitivity of esophageal cancer TE-1 cells and this effect was possibly through the activation of PTEN;MicroRNA 21 miR 21 expression promotes growth metastasis and chemo or radioresistance in non small cell lung cancer cells by targeting PTEN; Taken together these results provide evidence to show the promotion role of miR-21 in NSCLC development through modulation of the PTEN signaling pathway;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Role of microRNA 21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma; To evaluate the role of miR-21 and PTEN cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells; In Eca109 when transfected with miR-21 mimics accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40% which resulted in the promotion of cell proliferation; However when transfected with miR-21 inhibitor expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79% which resulted in the suppression of cell proliferation; Furthermore there was a significantly inverse correlation between miR-21 expression and PTEN protein levels p < 0.05; The author concluded that MiR-21 was overexpressed in vitro and ESCC and promoted the cell proliferation might target PTEN at post-transcriptional level and regulated the cancer invasion in Kazakh's ESCC;Difluorinated curcumin CDF restores PTEN expression in colon cancer cells by down regulating miR 21; Indeed our current data demonstrate a marked downregulation of PTEN in SCID mice xenografts of miR-21 over-expressing colon cancer HCT116 cells; Colonospheres that are highly enriched in cancer stem/stem like cells reveal increased miR-21 expression and decreased PTEN; Difluorinated curcumin CDF a novel analog of the dietary ingredient curcumin which has been shown to inhibit the growth of 5-Flurouracil + Oxaliplatin resistant colon cancer cells downregulated miR-21 in chemo-resistant colon cancer HCT116 and HT-29 cells and restored PTEN levels with subsequent reduction in Akt phosphorylation;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells;The results showed that knockdown of miR-21 by antagomir-21 decreased cell proliferation and induced apoptosis via targeting PTEN both in 4T1 cells and HUVECs;Finally invasion and metastasis assays were performed and alteration in mir-21 PTEN AKT and pAKT level was evaluated in these cells; Enhanced invasion and metastasis increased miR-21 expression decreased PTEN elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells;In the present study we investigated the role of miR-21 and its potential as a therapeutic target in two prostate cancer cell lines characterized by different miR-21 expression levels and PTEN gene status;Expressions of microRNA-21 miR-21 PTEN MMP9 and p47 were detected by qPCR;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;MiR-21 upregulation contributes to PTEN downregulation which is beneficial for the activation of PI3K/AKT signaling;microRNA 21 Regulates Cell Proliferation and Migration and Cross Talk with PTEN and p53 in Bladder Cancer; MicroRNA-21 regulates proliferation and migration of bladder cancer cells and cross talk with PTEN and p53 in bladder cancer;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status; To explore whether miR-21 can serve as a therapeutic target for glioblastoma we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 PTEN mutant and LN229 PTEN wild-type GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo; Taken together our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated; In conclusion our data suggest that mUA can suppress cell viability in DU145 cells through modulating miR-21 and its downstream series-wound targets including PTEN Akt and Wnt/β-catenin signaling;miR 21 confers cisplatin resistance in gastric cancer cells by regulating PTEN; In addition miR-21 induced cell survival and cisplatin resistance through downregulating the expression of phosphatase and tension homolog deleted on chromosome 10 PTEN and activation of Akt pathway;Finally we demonstrate that modulation of tumor suppressors PTEN and p53 in U87 cells does not affect the decrease in miR-21 levels associated with PDGF-B overexpression;This study aimed to investigate whether NSCLC miR-21 mediated resistance to TKIs also results from Pten targeting; Here we show miR-21 promotes cancer by negatively regulating Pten expression in human NSCLC tissues: high miR-21 expression levels were associated with shorter DFS in 47 NSCLC patients; high miR-21/low Pten expression levels indicated a poor TKI clinical response and shorter overall survival in another 46 NSCLC patients undergoing TKI treatment; In vitro assays showed that miR-21 was up-regulated concomitantly to down-regulation of Pten in pc-9/GR cells in comparison with pc-9 cells; Moreover over-expression of miR-21 significantly decreased gefitinib sensitivity by down-regulating Pten expression and activating Akt and ERK pathways in pc-9 cells while miR-21 knockdown dramatically restored gefitinib sensitivity of pc-9/GR cells by up-regulation of Pten expression and inactivation of AKT and ERK pathways in vivo and in vitro;MicroRNA 21 miR 21 represses tumor suppressor PTEN and promotes growth and invasion in non small cell lung cancer NSCLC; We identified the role of miR-21 in non-small cell lung cancer NSCLC and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation; Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot respectively; Tumor tissues showed an inverse correlation between miR-21 and PTEN protein; miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines; miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC;MiR-21 overexpression decreases PTEN increases p-Akt and subsequently increases HIF-1α expression while miR-21 inhibition results in increased PTEN decreased p-Akt and then decreased HIF-1α;Furthermore miR-21-induced upregulation of CSF-1 mRNA and its transcription were prevented by expression of PTEN mRNA lacking 3'-untranslated region UTR and miR-21 recognition sequence; Our results reveal a novel mechanism for the therapeutic function of fish oil diet that blocks miR-21 thereby increasing PTEN levels to prevent expression of CSF-1 in breast cancer;This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma DLBCL paraffin-embedded tissues and evaluate its potential relevance with clinical characteristics; In patients with DLBCL the expression level of miR-21 was negatively correlated with the level of PTEN protein; These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL;Down regulation of PTEN expression modulated by dysregulated miR 21 contributes to the progression of esophageal cancer; We demonstrated that knockdown of miR-21 significantly increased expression of PTEN protein; Our findings suggest that miR-21 could be a potential oncomiR probably by regulation of PTEN and a novel prognostic factor for ESCC patients;The levels of mir-21 did not associate with the expression of PTEN an important tumour suppressor in CRC and one of many putative targets of miR-21 but interestingly was associated with stage of disease in the PTEN expressing tumours;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants;The PTEN protein levels in CRC tissues and cells had an inverse correlation with miR-21 expression; MiR-21 targets PTEN at the post-transcriptional level and regulates cell proliferation and invasion in CRC;After miR-21 was transfected in MCF-7 cells PTEN protein level was measured by Western blot; Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt resulting in accumulation of Bad p21/WAF1/CIP1 and p27/KIP1;To further determine the potential involvement of miR-21 in breast cancer we have evaluated the expression level of miR-21 by stem-loop real-time RT-PCR based on SYBR-Green I in human invasive ductal carcinoma of the breast and we have correlated the results with clinicopathologic features and PTEN protein expression; The expression levels of miR-21 were correlated with PTEN and commonly used clinicopathologic features of breast cancer; Expression of miR-21 was negatively correlated with expression of PTEN P=0.013; These findings suggest that PTEN is possibly one of the targets of miR-21 in breast cancer and high expression of mir-21 indicates a more aggressive phenotype;These findings demonstrate a novel role of AR in the regulation of miR-21 and its target PTEN in growth factor-induced colon cancer cell growth;Whether miR-21 regulated PTEN expression was assessed by luciferase assay; The RNA and protein levels of PTEN were significantly decreased by exogenous miR-21 and the 3'-untranslated region of PTEN was shown to be a target of miR-21;In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX;microRNA 21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer; We performed a qRT-PCR assay with miR-21 and PTEN in 16 paired EEC tumor tissues and adjacent non-tumor endometrium; To validate the putative binding site of miR-21 in the 3' untranslated region 3'-UTR of PTEN messenger RNA mRNA a dual-luciferase reporter assay was carried out; The upregulation of miR-21 led to a significant decrease in the PTEN protein expression level P=0.007; The downregulation of miR-21 led to a significant increase in PTEN protein P=0.002; In conclusion we demonstrated that the expression of PTEN protein but not mRNA was negatively directly regulated by miR-21 in the KLE cell line; The overexpression of miR-21 modulated EEC cell proliferation through the downregulation of PTEN;The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR 21 and promoter methylation; In this study we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC; PTEN mRNA level was negatively correlated with miR-21 level r = -0.595 P < 0.001; PTEN expression was also correlated directly with the PTEN mRNA level r = 0.583 P < 0.001 and conversely with miR-21 level r = -0.632 P < 0.001; This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;MicroRNA 21 controls hTERT via PTEN in human colorectal cancer cell proliferation; The aim of this study was to determine a role of microRNA-21 miRNA-21 in colorectal cancer CRC and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue PTEN;Relevance of miR 21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells; We identified the tumor suppressor gene PTEN as a target of miR-21 and determined the mechanism of its regulation throughout reporter construct plasmids; Using this model we analyzed the expression of miR-21 and PTEN as well as functional effects such as autophagy and apoptosis induction; In SiHa cells there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells; We conclude that miR-21 post-transcriptionally down-regulates the expression of PTEN to promote cell proliferation and cervical cancer cell survival progression;cell migration;;;;metastasis;differentiation;;tumorigenesis;;drug resistance;;;;;;;;;;;;;;drug resistance;drug resistance;;;;;;drug resistance;;;;;;metastasis;drug resistance;;;;;;;;metastasis;;;;;;;progression;malignant trasformation;;drug resistance;poor survival;;drug resistance;poor survival;;;;;progression;staging;;;;;;;;;;drug resistance;;poor survival liver cancer;liver cancer;liver cancer;liver cancer;breast cancer;gastric cancer;cervical and endocervical cancer;colon cancer;gastric cancer;breast cancer;bladder cancer;glioblastoma;breast cancer;colon cancer;gastric cancer;gastric cancer;colorectal cancer;breast cancer;lung cancer;glioblastoma;melanoma;lung squamous cell cancer;colorectal cancer;lung squamous cell cancer;breast cancer;B cell lymphoma;breast cancer;lung squamous cell cancer;thyroid cancer;breast cancer;breast cancer;esophageal cancer;glioblastoma;cervical and endocervical cancer;esophageal cancer;esophageal cancer;lung squamous cell cancer;pancreatic cancer;lung cancer;esophageal cancer;colon cancer;B cell lymphoma;colon cancer;breast cancer;pancreatic cancer;prostate cancer;lung cancer;gastric cancer;breast cancer;bladder cancer;B cell lymphoma;glioblastoma;prostate cancer;gastric cancer;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;cervical and endocervical cancer;breast cancer;B cell lymphoma;esophageal cancer;colorectal cancer;pancreatic cancer;colorectal cancer;breast cancer;breast cancer;colon cancer;lung squamous cell cancer;lung cancer;endometrial cancer;colorectal cancer;pancreatic cancer;colorectal cancer;cervical and endocervical cancer hsa-miR-21-5p PTPN3 1.51084295924322 1.54551622173259e-34 -1.09679913983405 1.08772189092188e-15 miRNAWalker2_validate -0.346268834460471 7.08819192499444e-12 NA NA NA hsa-miR-21-5p PTPRB 1.51084295924322 1.54551622173259e-34 -1.32542877067513 1.77250908183134e-12 mirMAP -0.468187150825792 1.0789974921566e-11 NA NA NA hsa-miR-21-5p PTPRT 1.51084295924322 1.54551622173259e-34 -1.89012666330824 4.99769771079087e-07 mirMAP -0.410670273096541 0.00313629325312729 NA NA NA hsa-miR-21-5p PURA 1.51084295924322 1.54551622173259e-34 -0.370395647075075 1.00532282271468e-06 miRNAWalker2_validate -0.198963437888801 1.97908484987669e-13 NA NA NA hsa-miR-21-5p RAB11FIP2 1.51084295924322 1.54551622173259e-34 0.0463066186430496 0.661781012741478 miRNAWalker2_validate -0.129344121684595 0.000750313312053913 NA NA NA hsa-miR-21-5p RAB30 1.51084295924322 1.54551622173259e-34 -0.688840167280648 5.94886883391515e-06 mirMAP -0.331290882284831 1.82601971707362e-09 NA NA NA hsa-miR-21-5p RAB6A 1.51084295924322 1.54551622173259e-34 -0.425231925971688 6.4816140566381e-09 miRNAWalker2_validate;miRNATAP -0.10348788977009 0.000122896644917287 NA NA NA hsa-miR-21-5p RAB6C 1.51084295924322 1.54551622173259e-34 -0.589269355987388 5.0901403406691e-07 miRNAWalker2_validate -0.156086876111219 0.000298090383111658 NA NA NA hsa-miR-21-5p RALGPS2 1.51084295924322 1.54551622173259e-34 -0.265145862108441 0.0452541902055679 miRNAWalker2_validate;MirTarget -0.298500023178292 2.94960664606271e-10 NA NA NA hsa-miR-21-5p RAPGEF6 1.51084295924322 1.54551622173259e-34 -0.492574631515547 0.0475932470995822 miRNAWalker2_validate -0.275740488474668 0.00236685464406788 NA NA NA hsa-miR-21-5p RAPH1 1.51084295924322 1.54551622173259e-34 -0.828501491561341 2.21189027809936e-08 miRNAWalker2_validate;mirMAP -0.502969139994053 5.88322638281878e-22 NA NA NA hsa-miR-221-3p RAPH1 1.12244756358612 3.48370308238012e-13 -0.828501491561341 2.21189027809936e-08 mirMAP -0.245161829914477 7.98532935115403e-08 NA NA NA hsa-miR-21-5p RASA1 1.51084295924322 1.54551622173259e-34 -0.0728742068751655 0.561743113598097 miRNATAP -0.101935589192876 0.0258841950427365 25663768;27101583 RKO cells were transfected with vectors overexpressing or down-regulating either miR-21 or RASA1; Furthermore a luciferase reporter assay was used to determine whether RASA1 is a gene target of miR-21; RASA1 protein levels were significantly decreased in RKO cells compared with the other 5 colon cancer cell lines and RASA1 was confirmed as a target gene of miR-21; Interestingly RASA1 mRNA and protein levels in pre-miR-21-LV up-regulation of miR-21 cells were lower than those in anti-miR-21-LV down-regulation of miR-21 cells P < 0.05; RASA1 is a target gene of miR-21 which promotes malignant behaviors of RKO cells through regulation of RASA1 expression;Circulating MicroRNA 21 Is Involved in Lymph Node Metastasis in Cervical Cancer by Targeting RASA1; Then cervical cancer cell lines HeLa HPV-18 DNA E6/E7RNA and HT-3 HPV DNA E6/E7RNA were used to confirm the interaction between miR-21 and RASA1; MicroRNA-21 reduces RASA1 expression in cervical cancer cell lines and promotes cervical cancer cell migration via RASA1 malignant trasformation;metastasis;cell migration colon cancer;cervical and endocervical cancer hsa-miR-21-5p RB1 1.51084295924322 1.54551622173259e-34 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate -0.130479458630801 0.00994795669328339 NA NA NA hsa-miR-21-3p RCBTB1 -0.484219378716006 0.00299941098417659 0.323233540069233 0.0104156978634511 MirTarget -0.182012790140924 1.05705110744651e-06 NA NA NA hsa-miR-21-5p RDH11 1.51084295924322 1.54551622173259e-34 -0.412846891947739 0.000434475737209474 miRNAWalker2_validate -0.242728961766966 9.83085113436111e-09 NA NA NA hsa-miR-21-5p RECK 1.51084295924322 1.54551622173259e-34 -0.815315461427365 1.96892620421425e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.243949385419305 0.000503969331428142 25720799;19730150;20480266;22642976;18794849 We verified that HMGB1-induced expression of miR-21 in HCC provides a posttranscriptional repression of the matrix metalloproteinase MMP inhibitors RECK and TIMP3 which are known to impact HCC progression and metastases; Finally we found that inhibition of miR-21 in murine HMGB1-overexpressing HCC xenografts led to reduced tumor MMP activity through released repression of the miR-21 targets RECK and TIMP3 which ultimately impeded tumor progression;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;Furthermore we identified that RECK reversion-inducing-cysteine-rich protein with kazal motifs a tumor suppressor gene was a direct target of miR-21; Our results suggest that miR-21 expression has a key role in regulating cellular processes in osteosarcoma likely through regulating RECK and may serve as a therapeutic target;miR 21 may acts as an oncomir by targeting RECK a matrix metalloproteinase regulator in prostate cancer; The micro RNA miRNA mir-21 has been shown to regulate invasiveness in cancer through translational repression of the Metaloproteinase MMP inhibitor RECK; Our aim is to investigate the levels of expression of RECK and miR-21 in PCa comparing with classical prognostic factors and disease outcome and also test if RECK is a target of miR-21 in in vitro study using PCa cell line; To determine if RECK is a target of miR-21 in prostate cancer we performed an in vitro assay with PCa cell line DU-145 transfected with pre-miR-21 and anti-miR-21; To determine miR-21 and RECK expression levels in PCa samples we performed quantitative real-time polymerase chain reaction qRT-PCR; From men who presented the second profile miR-21 overexpression and RECK underexpression 91.7% were staged pT3; Our results demonstrate RECK as a target of miR-21; We believe that miR-21 may be important in PCa progression through its regulation of RECK a known regulator of tumor cell invasion;Finally we showed that RECK a known tumor suppressor in gastric cancer is a bona fide target of miR-21; Taken together miR-21 may be important in the initiation and progression of gastric cancers as an oncomiR likely through regulating RECK; Our findings suggest a potential regulatory pathway in which H pylori infection upregulates expression of miR-21 which in turn downregulates RECK and then leads to the development of gastric cancer metastasis;progression;;;staging;progression;progression liver cancer;pancreatic cancer;sarcoma;prostate cancer;gastric cancer hsa-miR-21-5p REEP5 1.51084295924322 1.54551622173259e-34 -0.41097136505496 2.30189092122078e-06 mirMAP -0.103494196589679 0.00118513348610856 NA NA NA hsa-miR-21-5p REST 1.51084295924322 1.54551622173259e-34 -1.00246189990012 0.000168942614777061 miRNAWalker2_validate;miRTarBase;mirMAP -0.485307393654179 5.49786816225102e-07 NA NA NA hsa-miR-21-5p REV1 1.51084295924322 1.54551622173259e-34 -0.0941519895887022 0.175117002553565 miRNAWalker2_validate -0.135012076309716 5.30171003159604e-08 NA NA NA hsa-miR-21-5p REV3L 1.51084295924322 1.54551622173259e-34 -0.483702621543161 0.000164111273212257 miRNAWalker2_validate -0.292308331723035 2.44981895454986e-10 NA NA NA hsa-miR-221-3p REV3L 1.12244756358612 3.48370308238012e-13 -0.483702621543161 0.000164111273212257 miRNATAP -0.141261854229422 0.00035348728523936 NA NA NA hsa-miR-21-5p RHOB 1.51084295924322 1.54551622173259e-34 -1.7358770639627 5.43143135520556e-23 miRNAWalker2_validate;miRTarBase;miRNATAP -0.561330691774606 7.06300169113094e-18 21872591;25861727 miR 21 targets the tumor suppressor RhoB and regulates proliferation invasion and apoptosis in colorectal cancer cells; Here we show that expression of miR-21 in HEK293 and several colorectal cancer cells was found inversely correlated with ras homolog gene family member B RhoB expression; miR-21 expression significantly suppressed RhoB 3' UTR luciferase-reporter activity but the inhibitory effect was lost when the putative target sites were mutated; Exogenous miR-21 over-expression mimicked the effect of RhoB knockdown in promoting proliferation and invasion and inhibiting apoptosis whereas anti-miR-21 or RhoB expression yielded opposite effects in colorectal cancer cells; These results suggest that miR-21 is a regulator of RhoB expression and RhoB could be a useful target in exploring the potential therapeutic benefits of miR-21 mediated tumor cell behaviors in colorectal cancer;Here we demonstrate that intravenously-administered chlorotoxin CTX-coupled targeted stable nucleic acid lipid particle SNALP-formulated anti-miR-21 oligonucleotides accumulate preferentially within brain tumors and promote efficient miR-21 silencing which results in increased mRNA and protein levels of its target RhoB while showing no signs of systemic immunogenicity ; colorectal cancer;glioblastoma hsa-miR-21-5p RMND5A 1.51084295924322 1.54551622173259e-34 -0.492348577397466 7.84892428586817e-07 miRNAWalker2_validate;MirTarget;miRNATAP -0.403003232389479 2.03513171913892e-32 NA NA NA hsa-miR-21-5p RNF103 1.51084295924322 1.54551622173259e-34 -0.254183630226668 0.00489792672661882 MirTarget -0.159966535927483 9.34956974467895e-07 NA NA NA hsa-miR-21-5p RNF11 1.51084295924322 1.54551622173259e-34 -0.638411154558487 5.72201685892189e-13 miRNAWalker2_validate -0.255585765765577 1.58574927655847e-15 NA NA NA hsa-miR-21-5p RNF111 1.51084295924322 1.54551622173259e-34 -0.210320255430396 0.0161550339003716 miRNATAP -0.135087044530108 1.94177510649676e-05 NA NA NA hsa-miR-21-3p RNF128 -0.484219378716006 0.00299941098417659 -0.731583017031184 9.18291340209668e-08 MirTarget -0.109694895325289 0.00811169468290333 NA NA NA hsa-miR-221-3p RNF152 1.12244756358612 3.48370308238012e-13 -1.99846276106589 3.29375349002461e-27 mirMAP -0.529799845859018 7.66563669791234e-20 NA NA NA hsa-miR-21-3p RORA -0.484219378716006 0.00299941098417659 -1.18591701647076 1.08163415927424e-11 mirMAP -0.199363778779816 0.000177270450725578 NA NA NA hsa-miR-21-5p RP2 1.51084295924322 1.54551622173259e-34 -0.819209747170963 6.4575408341589e-09 miRNAWalker2_validate;miRNATAP -0.242080603331582 3.16543614744208e-06 NA NA NA hsa-miR-21-3p RRAGB -0.484219378716006 0.00299941098417659 0.359547665063954 0.000344467681392019 MirTarget -0.127751893517882 1.87937874574002e-05 NA NA NA hsa-miR-21-5p RSBN1 1.51084295924322 1.54551622173259e-34 -0.449018724973878 5.46712303920279e-08 miRNATAP -0.161692680668615 7.78930500169261e-08 NA NA NA hsa-miR-21-5p RSF1 1.51084295924322 1.54551622173259e-34 -0.42651865559777 1.69657930050886e-05 miRNAWalker2_validate -0.223277086683801 4.14888167197538e-10 NA NA NA hsa-miR-21-5p RSPRY1 1.51084295924322 1.54551622173259e-34 -0.728312362542739 0.000260269767961343 miRNAWalker2_validate -0.287552771807177 7.92783272600922e-05 NA NA NA hsa-miR-21-5p RTN4 1.51084295924322 1.54551622173259e-34 -0.737552092106895 3.21720282494762e-12 miRNAWalker2_validate;miRTarBase -0.270647009690661 2.41797685711415e-12 NA NA NA hsa-miR-221-3p RUNDC3B 1.12244756358612 3.48370308238012e-13 -1.77231643639114 1.974302394488e-11 miRNAWalker2_validate -0.644731514464414 1.20643721256973e-15 NA NA NA hsa-miR-21-5p SACM1L 1.51084295924322 1.54551622173259e-34 -0.315204603662408 1.78726511835303e-06 miRNAWalker2_validate -0.236338892627455 3.65128683570502e-25 NA NA NA hsa-miR-221-3p SAMD5 1.12244756358612 3.48370308238012e-13 -3.18008032786867 6.30217447905566e-16 mirMAP -0.71879613241747 6.03121839066379e-09 NA NA NA hsa-miR-21-5p SAMD8 1.51084295924322 1.54551622173259e-34 -0.660046840397926 4.02497533644099e-07 mirMAP -0.176847269609028 0.000224590266324053 NA NA NA hsa-miR-21-5p SAR1B 1.51084295924322 1.54551622173259e-34 -0.602691569549908 4.60715859176311e-06 mirMAP -0.285426487057633 2.07980510331109e-09 NA NA NA hsa-miR-21-5p SASH1 1.51084295924322 1.54551622173259e-34 -0.641621637334084 3.36245859697969e-07 miRNAWalker2_validate;MirTarget -0.229739176617252 5.80981721365785e-07 NA NA NA hsa-miR-21-5p SATB1 1.51084295924322 1.54551622173259e-34 -1.66235785428604 6.97431443036821e-14 MirTarget;miRNATAP -0.340471853554043 4.20923938471208e-05 27107063 SATB1 is Down regulated in Clear Cell Renal Cell Carcinoma and Correlates with miR 21 5p Overexpression and Poor Prognosis; This study aimed to determine the expression levels of SATB1 as well as miR-21-5p -the post-transcriptional repressor of SATB1 expression- in clear cell renal cell carcinoma ccRCC and to investigate their association with the progression of ccRCC; Immunohistochemistry and quantitative polymerase chain reaction were used to assess the expression of SATB1 protein and mRNA as well as miR-21-5p in tumor and matched normal kidney tissues collected from 56 ccRCC patients; SATB1 mRNA level was down-regulated in ccRCC tissue and inversely correlated with the content of miR-21-5p; Down-regulation of SATB1 mRNA and up-regulation of miR-21-5p were associated with shorter patient survival; Decreased expression of SATB1 in ccRCC may result from over-expressed miR-21-5p worse prognosis;progression;poor survival kidney renal cell cancer hsa-miR-21-5p SBNO1 1.51084295924322 1.54551622173259e-34 -0.665259965543963 0.0143028966764536 mirMAP -0.404488518211087 4.20992560960646e-05 NA NA NA hsa-miR-21-3p SCAI -0.484219378716006 0.00299941098417659 0.00158497185467299 0.987324440239739 MirTarget -0.210575694006648 2.74097896019217e-13 NA NA NA hsa-miR-21-5p SCN8A 1.51084295924322 1.54551622173259e-34 -0.0533720825097165 0.8138851401538 miRNATAP -0.346698629870095 2.40168964461047e-05 NA NA NA hsa-miR-21-3p SDC2 -0.484219378716006 0.00299941098417659 -0.03087902282995 0.814769949243076 MirTarget -0.229018223179984 2.83112934657942e-09 NA NA NA hsa-miR-21-5p SEC14L5 1.51084295924322 1.54551622173259e-34 0.311980940356044 0.259327528499545 mirMAP -0.240988967484998 0.016991558309477 NA NA NA hsa-miR-21-5p SEC62 1.51084295924322 1.54551622173259e-34 -0.921906590113964 6.87913526550551e-31 mirMAP -0.331904493045303 4.15393465049971e-30 NA NA NA hsa-miR-221-3p SEC62 1.12244756358612 3.48370308238012e-13 -0.921906590113964 6.87913526550551e-31 miRNATAP -0.184748336432645 6.05137407387986e-13 NA NA NA hsa-miR-21-5p SEC63 1.51084295924322 1.54551622173259e-34 -0.325812925019161 4.09550265143784e-05 miRNAWalker2_validate;MirTarget -0.145396208223142 4.30963773886017e-07 NA NA NA hsa-miR-221-3p SELE 1.12244756358612 3.48370308238012e-13 -2.35025100185437 3.2370278829201e-13 miRNAWalker2_validate -0.52896422861731 1.63448650320114e-07 NA NA NA hsa-miR-21-5p SEMA5A 1.51084295924322 1.54551622173259e-34 -1.1432007554504 2.30205619351887e-07 miRNAWalker2_validate -0.360641166634172 8.92713073935218e-06 NA NA NA hsa-miR-221-3p SEMA5A 1.12244756358612 3.48370308238012e-13 -1.1432007554504 2.30205619351887e-07 mirMAP -0.457359982916681 1.11177738447042e-11 NA NA NA hsa-miR-221-3p SEMA6D 1.12244756358612 3.48370308238012e-13 -1.73527979620398 1.31444245208132e-15 mirMAP;miRNATAP -0.307723746797562 7.41427256696092e-06 NA NA NA hsa-miR-21-5p SESN1 1.51084295924322 1.54551622173259e-34 -0.582017748855085 1.56850305646972e-06 miRNAWalker2_validate -0.309095857741543 1.28235786632969e-12 NA NA NA hsa-miR-21-5p SFXN1 1.51084295924322 1.54551622173259e-34 -0.990318290801911 5.86324585320089e-17 miRNAWalker2_validate -0.394828254121738 3.12485232263999e-20 NA NA NA hsa-miR-21-5p SGCB 1.51084295924322 1.54551622173259e-34 -0.991863016074027 7.13504872359113e-06 miRNAWalker2_validate -0.408063134168544 4.09056141654257e-07 NA NA NA hsa-miR-21-5p SGK3 1.51084295924322 1.54551622173259e-34 -0.235888881337767 0.0629928865889445 miRNAWalker2_validate -0.158120464091527 0.000607322860528501 NA NA NA hsa-miR-21-5p SH3BGRL2 1.51084295924322 1.54551622173259e-34 -0.967834191230422 9.67498247511188e-10 mirMAP -0.509292184634394 1.69575741221457e-19 NA NA NA hsa-miR-21-5p SIKE1 1.51084295924322 1.54551622173259e-34 -0.25247843404849 0.000535002945958211 mirMAP -0.163668491824083 3.51523764041468e-10 NA NA NA hsa-miR-21-5p SKI 1.51084295924322 1.54551622173259e-34 -0.298904352736227 0.00375499820753305 MirTarget;miRNATAP -0.257048686823694 2.62914030320397e-12 NA NA NA hsa-miR-21-5p SKP2 1.51084295924322 1.54551622173259e-34 0.422595289761893 0.0200334942687184 miRNAWalker2_validate;MirTarget -0.199555879642397 0.00262016397392478 NA NA NA hsa-miR-21-5p SLAIN2 1.51084295924322 1.54551622173259e-34 -0.37865756950093 8.79283914288049e-08 miRNAWalker2_validate -0.16620781375253 7.34738059339728e-11 NA NA NA hsa-miR-21-5p SLC16A10 1.51084295924322 1.54551622173259e-34 -1.40592334895919 3.67016779027723e-07 miRNAWalker2_validate -0.637594864437139 2.07184643565534e-10 NA NA NA hsa-miR-21-3p SLC1A2 -0.484219378716006 0.00299941098417659 -2.05184743338733 5.07102901300161e-06 mirMAP -0.335196053665038 0.0135639113506395 NA NA NA hsa-miR-21-5p SLC30A10 1.51084295924322 1.54551622173259e-34 -0.0543453064095001 0.81132950410964 MirTarget -0.33819710274008 4.12133043822169e-05 NA NA NA hsa-miR-21-5p SLC31A1 1.51084295924322 1.54551622173259e-34 -1.14463479413429 2.19129504521951e-22 miRNAWalker2_validate -0.495672539696826 2.26515771240959e-32 NA NA NA hsa-miR-21-5p SLC35F5 1.51084295924322 1.54551622173259e-34 -0.347747668560416 0.000413266412082304 MirTarget -0.183358853918835 2.6404634334924e-07 NA NA NA hsa-miR-221-3p SLC4A4 1.12244756358612 3.48370308238012e-13 -2.23317622565422 7.81779722860372e-13 miRNATAP -0.784979956472366 1.6201625395874e-16 NA NA NA hsa-miR-21-3p SLC5A3 -0.484219378716006 0.00299941098417659 -1.14202730091157 9.93875965261116e-13 mirMAP -0.10029245756377 0.041524733723636 NA NA NA hsa-miR-21-5p SLC5A3 1.51084295924322 1.54551622173259e-34 -1.14202730091157 9.93875965261116e-13 miRNAWalker2_validate -0.537351225066056 6.71065741144818e-21 NA NA NA hsa-miR-221-3p SLC5A3 1.12244756358612 3.48370308238012e-13 -1.14202730091157 9.93875965261116e-13 mirMAP -0.143811301491153 0.00447982622806733 NA NA NA hsa-miR-21-5p SLK 1.51084295924322 1.54551622173259e-34 -0.352886234776743 0.000875676142405013 miRNAWalker2_validate -0.159745655335613 3.43594750142475e-05 NA NA NA hsa-miR-21-3p SMAD7 -0.484219378716006 0.00299941098417659 -0.723856409040082 1.50332971335389e-07 MirTarget -0.101250788505418 0.0151930427299818 23372687;27185036;26531758 Furthermore the expression of MSH2 and SMAD7 two important molecules involving TGF-β pathway was restored following miR-21 knockdown in both MCF-7 and Hs578T breast cancer cells;MicroRNA 21 Regulates Non Small Cell Lung Cancer Cell Invasion and Chemo Sensitivity through SMAD7; We performed bioinformatics analyses on the binding of microRNA-21 miR-21 to the 3'-UTR of SMAD7 mRNA and verified the biological effects of this binding using promoter luciferase reporter assay; Furthermore expression of miR-21 was found to be inhibited by Carboplatin and bioinformatics analyses showed that miR-21 targeted the 3'-UTR of SMAD7 mRNA to inhibit its translation which was confirmed by luciferase reporter assay; Carboplatin may upregulate SMAD7 through suppression of miR-21 to inhibit TGFβ receptor signaling mediated NSCLC cell invasion;MicroRNA 21 induces breast cancer cell invasion and migration by suppressing smad7 via EGF and TGF β pathways; The present study was undertaken to determine the association of miR-21 smad7 EGF and TGF-β with breast cancer cell invasion and migration and to identify the molecular mechanisms involved using immunohistochemistry and western blot analysis; Smad7 was confirmed to be a direct target of miR-21 by luciferase reporter and western blot assays; The downregulation of smad7 by miR-21 or sismad7 enhanced EGF-dependent invasion and migration as well as TGF-β-dependent invasion and migration; The actions of miR-21 were abrogated by expressing a modified smad7 cDNA resistant to miR-21; In conclusion our results demonstrated that plasma miR-21 levels may serve as a diagnostic marker in breast cancers whereas miR-21 promotes breast cancer cell proliferation and invasion by suppressing smad7 which enhances EGF and TGF-β pathways ;; breast cancer;lung squamous cell cancer;breast cancer hsa-miR-21-5p SMAD7 1.51084295924322 1.54551622173259e-34 -0.723856409040082 1.50332971335389e-07 miRNAWalker2_validate;MirTarget;miRNATAP -0.273576086802476 5.38840691437798e-08 23372687;27185036;26531758 Furthermore the expression of MSH2 and SMAD7 two important molecules involving TGF-β pathway was restored following miR-21 knockdown in both MCF-7 and Hs578T breast cancer cells;MicroRNA 21 Regulates Non Small Cell Lung Cancer Cell Invasion and Chemo Sensitivity through SMAD7; We performed bioinformatics analyses on the binding of microRNA-21 miR-21 to the 3'-UTR of SMAD7 mRNA and verified the biological effects of this binding using promoter luciferase reporter assay; Furthermore expression of miR-21 was found to be inhibited by Carboplatin and bioinformatics analyses showed that miR-21 targeted the 3'-UTR of SMAD7 mRNA to inhibit its translation which was confirmed by luciferase reporter assay; Carboplatin may upregulate SMAD7 through suppression of miR-21 to inhibit TGFβ receptor signaling mediated NSCLC cell invasion;MicroRNA 21 induces breast cancer cell invasion and migration by suppressing smad7 via EGF and TGF β pathways; The present study was undertaken to determine the association of miR-21 smad7 EGF and TGF-β with breast cancer cell invasion and migration and to identify the molecular mechanisms involved using immunohistochemistry and western blot analysis; Smad7 was confirmed to be a direct target of miR-21 by luciferase reporter and western blot assays; The downregulation of smad7 by miR-21 or sismad7 enhanced EGF-dependent invasion and migration as well as TGF-β-dependent invasion and migration; The actions of miR-21 were abrogated by expressing a modified smad7 cDNA resistant to miR-21; In conclusion our results demonstrated that plasma miR-21 levels may serve as a diagnostic marker in breast cancers whereas miR-21 promotes breast cancer cell proliferation and invasion by suppressing smad7 which enhances EGF and TGF-β pathways ;; breast cancer;lung squamous cell cancer;breast cancer hsa-miR-21-5p SNED1 1.51084295924322 1.54551622173259e-34 -0.697753140844763 1.06949754459612e-05 miRNATAP -0.573975270011572 1.6651715194934e-25 NA NA NA hsa-miR-21-5p SNRK 1.51084295924322 1.54551622173259e-34 -0.85262800892875 3.46352510534433e-18 miRNAWalker2_validate -0.413281409472092 2.81445266762145e-33 NA NA NA hsa-miR-21-5p SNX1 1.51084295924322 1.54551622173259e-34 -0.199729686068656 0.00561090290533367 mirMAP -0.148423358085664 9.057605348543e-09 NA NA NA hsa-miR-221-3p SNX4 1.12244756358612 3.48370308238012e-13 -0.536100687729702 1.28915337553997e-11 MirTarget -0.149748699874215 8.48339420719281e-10 NA NA NA hsa-miR-221-3p SOD2 1.12244756358612 3.48370308238012e-13 -0.690525669767364 9.88917819083763e-05 MirTarget -0.307899581648328 1.25664929027329e-08 NA NA NA hsa-miR-21-5p SOS2 1.51084295924322 1.54551622173259e-34 -0.326258424146275 0.000900714126323724 miRNATAP -0.279899776012046 7.56477883436428e-16 NA NA NA hsa-miR-21-5p SOX5 1.51084295924322 1.54551622173259e-34 -1.35870465626211 3.34572839723252e-09 miRNAWalker2_validate;miRTarBase;miRNATAP -0.752563630948822 3.02619719501452e-20 NA NA NA hsa-miR-21-5p SOX6 1.51084295924322 1.54551622173259e-34 -2.13149609157814 4.50445986535194e-12 MirTarget;miRNATAP -0.442546399262666 0.00011832613787611 NA NA NA hsa-miR-21-5p SOX7 1.51084295924322 1.54551622173259e-34 -0.986286162209553 5.18629323126467e-07 miRNATAP -0.456800368812046 1.40622099782934e-10 NA NA NA hsa-miR-21-5p SPATA18 1.51084295924322 1.54551622173259e-34 -2.42440573416054 6.14787335383988e-13 mirMAP -0.888168123228807 5.84448638593106e-13 NA NA NA hsa-miR-21-5p SPG11 1.51084295924322 1.54551622173259e-34 -0.217404490510732 0.0127089782026275 miRNAWalker2_validate -0.131221875348133 3.25452731693718e-05 NA NA NA hsa-miR-21-5p SPG20 1.51084295924322 1.54551622173259e-34 -2.0188037720553 2.56848033710916e-20 miRNATAP -0.36514192341709 1.12103205042907e-05 NA NA NA hsa-miR-21-5p SPIN1 1.51084295924322 1.54551622173259e-34 -0.173743578973942 0.107261524360596 miRNAWalker2_validate;miRNATAP -0.146320667513631 0.000182886372082751 NA NA NA hsa-miR-21-5p SPRY1 1.51084295924322 1.54551622173259e-34 -0.191200095311501 0.161703034220406 miRNATAP -0.14613864441268 0.00329886441657982 24155920 Functional studies showed that miR-21 over-expression in GICs induced comparable cell differentiation features and targeted SPRY1 mRNA which encodes for a negative regulator of neural stem-cell differentiation differentiation glioblastoma hsa-miR-21-5p SPRY2 1.51084295924322 1.54551622173259e-34 -1.61009043608154 1.29635420799403e-31 miRNAWalker2_validate;miRTarBase;miRNATAP -0.425735867826972 4.40806254549318e-16 22322462 Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon tumorigenesis colon cancer hsa-miR-21-5p SPTLC3 1.51084295924322 1.54551622173259e-34 -1.10803369124109 1.07050221229424e-07 miRNAWalker2_validate -0.301824182256997 8.61062447291768e-05 NA NA NA hsa-miR-21-5p SPTY2D1 1.51084295924322 1.54551622173259e-34 -0.240993112197188 0.000856993759157496 mirMAP -0.102380754626533 9.42383843828826e-05 NA NA NA hsa-miR-21-5p SSFA2 1.51084295924322 1.54551622173259e-34 -1.04867750273814 9.35976525656476e-18 miRNAWalker2_validate;miRNATAP -0.440458857136484 1.21522358351572e-23 NA NA NA hsa-miR-21-3p ST3GAL1 -0.484219378716006 0.00299941098417659 -0.767532914178657 6.27233158530294e-05 MirTarget -0.181888009049488 0.00157399211019762 NA NA NA hsa-miR-21-5p ST6GAL1 1.51084295924322 1.54551622173259e-34 -1.06038675540743 4.87924984478722e-10 miRNAWalker2_validate -0.381531456669473 9.47233085735488e-10 NA NA NA hsa-miR-21-5p ST8SIA3 1.51084295924322 1.54551622173259e-34 -2.69127578966183 3.01156225530941e-11 mirMAP -0.719320570916538 1.65097221003899e-06 NA NA NA hsa-miR-21-5p STAG2 1.51084295924322 1.54551622173259e-34 -0.0714198831818473 0.421841747970601 miRNAWalker2_validate;MirTarget;miRNATAP -0.137742528039037 1.73808146164746e-05 NA NA NA hsa-miR-21-3p STAT5B -0.484219378716006 0.00299941098417659 -0.206850819374259 0.00628123935115017 MirTarget -0.123627670641263 2.54618000627074e-08 NA NA NA hsa-miR-21-5p STK40 1.51084295924322 1.54551622173259e-34 -0.821847220109252 4.78818932052572e-18 MirTarget;miRNATAP -0.185491264902573 1.89215466830158e-07 NA NA NA hsa-miR-21-3p STX1B -0.484219378716006 0.00299941098417659 -0.640719876691996 0.00172836284903972 mirMAP -0.159305527804208 0.00931126450824499 NA NA NA hsa-miR-221-3p STX1B 1.12244756358612 3.48370308238012e-13 -0.640719876691996 0.00172836284903972 miRNATAP -0.457879211104611 8.3348808250142e-14 NA NA NA hsa-miR-221-3p SUN2 1.12244756358612 3.48370308238012e-13 -0.932850868223685 1.96552976055956e-16 miRNATAP -0.177235744649676 7.34315451746358e-07 NA NA NA hsa-miR-221-3p SYBU 1.12244756358612 3.48370308238012e-13 -1.03424280598767 8.76454515036323e-06 MirTarget;miRNATAP -0.533431254409308 2.80510282121204e-14 NA NA NA hsa-miR-21-5p SYNE2 1.51084295924322 1.54551622173259e-34 -0.596040988930904 1.98484771381191e-05 miRNAWalker2_validate -0.267941031650076 1.30549594293723e-07 NA NA NA hsa-miR-21-5p SYT15 1.51084295924322 1.54551622173259e-34 -0.962117365831715 0.000115018031669002 MirTarget -0.390773797269576 1.79311302132641e-05 NA NA NA hsa-miR-21-5p TAF1 1.51084295924322 1.54551622173259e-34 0.0712783642995616 0.555885290313746 miRNAWalker2_validate -0.138574765034134 0.0016161652445308 NA NA NA hsa-miR-221-3p TAOK1 1.12244756358612 3.48370308238012e-13 -0.395598965266263 0.0493024563419061 miRNATAP -0.15781076312396 0.0108208120585214 NA NA NA hsa-miR-21-3p TBC1D24 -0.484219378716006 0.00299941098417659 -0.0812041226045097 0.49898606857134 MirTarget -0.111066262618356 0.00184156647545758 NA NA NA hsa-miR-21-5p TCF21 1.51084295924322 1.54551622173259e-34 -2.50041580080969 5.23151011651186e-23 miRNAWalker2_validate -0.614038692243697 1.41229606334496e-10 NA NA NA hsa-miR-21-3p TEF -0.484219378716006 0.00299941098417659 0.525410771213851 0.000338359038626339 MirTarget -0.29082157580639 1.28174363293255e-11 NA NA NA hsa-miR-21-5p TESK2 1.51084295924322 1.54551622173259e-34 -1.01560084238235 8.87948423764961e-14 miRNAWalker2_validate;MirTarget;miRNATAP -0.507273804433523 6.74819091283339e-26 NA NA NA hsa-miR-21-5p TGFBR2 1.51084295924322 1.54551622173259e-34 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.236840105633096 2.41705180023436e-07 24037531 Androgen receptor and microRNA 21 axis downregulates transforming growth factor beta receptor II TGFBR2 expression in prostate cancer; Our results revealed that miR-21 suppresses TGFBR2 levels by binding to its 3'-UTR and AR signaling further potentiates this effect in both untransformed and transformed human prostate epithelial cells as well as in human prostate cancers; Manipulation of androgen signaling or the expression levels of AR or miR-21 negatively altered TGFBR2 expression in untransformed and transformed human prostate epithelial cells human prostate cancer xenografts and mouse prostate glands; Together these results suggest that the AR and miR-21 axis exerts its oncogenic effects in prostate tumors by downregulating TGFBR2 hence inhibiting the tumor-suppressive activity of TGFβ pathway prostate cancer hsa-miR-21-5p TGFBR3 1.51084295924322 1.54551622173259e-34 -1.53475612080717 2.32211754233392e-15 miRNAWalker2_validate -0.700530304569563 4.67202507056358e-24 NA NA NA hsa-miR-21-5p THRB 1.51084295924322 1.54551622173259e-34 -0.630015828817221 1.00813020178641e-06 MirTarget;miRNATAP -0.466517315998671 2.16976205749556e-25 21159845 Direct interaction with THRB was shown for miR-21 and miR-146a; We observed lower levels of THRB transcripts in cell lines transfected with miR-21 -146a and -221 down-regulation of 37-48%; P < 0.0001 but not with miR-181a; The analysis of tumor/normal tissue pairs revealed down-regulation of THRB in 11 of 13 pairs 1.3- to 9.1-fold and up-regulation of miR-21 -146a -181a and -221 in almost all pairs thyroid cancer hsa-miR-21-5p TIAM1 1.51084295924322 1.54551622173259e-34 -1.56411477781397 6.02866811798354e-13 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.321917397006639 7.35213503545411e-05 20826792 miR 21 and miR 31 converge on TIAM1 to regulate migration and invasion of colon carcinoma cells; We present compelling evidence that TIAM1 a guanidine exchange factor of the Rac GTPase is a direct target of both miR-21 and miR-31 colon cancer hsa-miR-221-3p TIAM1 1.12244756358612 3.48370308238012e-13 -1.56411477781397 6.02866811798354e-13 miRNAWalker2_validate -0.281862102724342 3.73338764540275e-05 NA NA NA hsa-miR-21-5p TIMP3 1.51084295924322 1.54551622173259e-34 -0.479569227754572 0.00042274706056242 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.285668268989237 5.87344757554495e-09 25720799;23936642;21820586;20346171;25587717 We verified that HMGB1-induced expression of miR-21 in HCC provides a posttranscriptional repression of the matrix metalloproteinase MMP inhibitors RECK and TIMP3 which are known to impact HCC progression and metastases; Finally we found that inhibition of miR-21 in murine HMGB1-overexpressing HCC xenografts led to reduced tumor MMP activity through released repression of the miR-21 targets RECK and TIMP3 which ultimately impeded tumor progression;Genetic heterogeneity of breast cancer metastasis may be related to miR 21 regulation of TIMP 3 in translation; The aim of this study is to determine whether microRNA-21 miR-21 a specific microRNA implicated in multiple aspects of carcinogenesis promoted breast cancer metastasis by regulating the tissue inhibitor of metalloproteinase 3 TIMP-3 gene; Correlation analysis was performed between miR-21 and TIMP-3; There was significantly inverse correlation between miR-21 and TIMP-3 extracted from tissue;The effect of TIMP3 on miR-21-induced cell survival was determined by transfection with TIMP3 lacking 3' untranslated region and miR-21;The aim of this study was to determine whether microRNA-21 miR-21 a specific microRNA implicated in multiple aspects of carcinogenesis impacts breast cancer invasion by regulating the tissue inhibitor of metalloproteinase 3 TIMP3 gene; In addition the regulation of tissue inhibitor of metalloproteinase 3 TIMP3 by miR-21 was evaluated by western blotting and luciferase assays; Evaluation of TIMP3 protein levels a peptidase involved in extarcellular matrix degredation inversely correlated with miR-21 expression; As knockdown of miR-21 increased TIMP3 protein expression and luciferase reporter activity our data suggests that miR-21 could promote invasion in breast cancer cells via its regulation of TIMP3;It was previously shown by our group that miR-21 a potential regulator of TIMP3 is over-expressed in cutaneous melanoma; It was therefore hypothesized that increased levels of miR-21 expression would lead to decreased expression of TIMP3 and thereby enhance the invasiveness of melanoma cells; Reduced expression of TIMP3 was achieved by siRNA knockdown and significantly enhanced invasion of melanoma cell lines mimicking the effects of miR-21 over-expression; Treatment of tumor cells with a linked nucleic acid antagomir to miR-21 inhibited tumor growth and increased tumor expression of TIMP3 in vivo in 01B74 Athymic NCr-nu/nu mice; This data shows that increased expression of miR-21 enhanced the invasive potential of melanoma cell lines through TIMP3 inhibition metastasis;progression;metastasis;tumorigenesis;poor survival;tumorigenesis; liver cancer;breast cancer;kidney renal cell cancer;breast cancer;melanoma hsa-miR-221-3p TIMP3 1.12244756358612 3.48370308238012e-13 -0.479569227754572 0.00042274706056242 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.144635283598806 0.000555681773778346 27077469 MiR 221 Exacerbate Cell Proliferation and Invasion by Targeting TIMP3 in Papillary Thyroid Carcinoma; The results indicated that miR-221 exacerbated PTC by downregulating the expression of TIMP3; The effects of miR-221 and TIMP3 in vivo were also confirmed by human PTC-bearing mice models which suggest consistent results with those in vitro studies thyroid cancer hsa-miR-221-3p TIPARP 1.12244756358612 3.48370308238012e-13 -0.956038686169793 4.14382659036917e-16 miRNAWalker2_validate -0.122527915469409 0.0010411994646257 NA NA NA hsa-miR-221-3p TLE3 1.12244756358612 3.48370308238012e-13 -0.222524741699439 0.182669026941518 MirTarget;miRNATAP -0.130688892291307 0.0108537727293893 NA NA NA hsa-miR-21-5p TLR4 1.51084295924322 1.54551622173259e-34 -1.6828344992865 2.89483284631908e-26 miRNAWalker2_validate -0.447384108410163 8.62007595677013e-14 27286259 For in vivo relevance expression of TLR4 was correlated with miR-21 expression and ROS production in freshly isolated untreated primary human lung cancer cells lung cancer hsa-miR-221-3p TMED7 1.12244756358612 3.48370308238012e-13 -0.253890513639785 0.00735487003225511 miRNAWalker2_validate;miRTarBase -0.129872834856252 6.87132678664697e-06 NA NA NA hsa-miR-21-5p TMEM170B 1.51084295924322 1.54551622173259e-34 -0.28665834823882 0.06540862544437 mirMAP -0.438717928720622 1.30887766625303e-15 NA NA NA hsa-miR-221-3p TMEM25 1.12244756358612 3.48370308238012e-13 -1.8651521520056 8.88146240297357e-13 MirTarget -0.655125853826896 2.01086228648022e-16 NA NA NA hsa-miR-21-5p TMEM56 1.51084295924322 1.54551622173259e-34 -1.75634225549522 4.44536598470124e-19 miRNAWalker2_validate;mirMAP -0.819689688932153 4.87190400354451e-32 NA NA NA hsa-miR-221-3p TMEM56 1.12244756358612 3.48370308238012e-13 -1.75634225549522 4.44536598470124e-19 mirMAP -0.479846425572342 4.93899541777324e-15 NA NA NA hsa-miR-221-3p TMEM64 1.12244756358612 3.48370308238012e-13 -0.00136604960589803 0.993609309009731 miRNAWalker2_validate -0.120583762709932 0.0213365728251542 NA NA NA hsa-miR-21-5p TNFAIP1 1.51084295924322 1.54551622173259e-34 -0.25456481631844 0.00166535803744622 mirMAP -0.134050497609676 4.70645131006535e-06 NA NA NA hsa-miR-221-3p TNFSF10 1.12244756358612 3.48370308238012e-13 -0.719605061671142 5.12756319214624e-05 miRNAWalker2_validate;miRTarBase -0.279691652886283 2.76653565272805e-07 NA NA NA hsa-miR-221-3p TNIP1 1.12244756358612 3.48370308238012e-13 0.0469734113262685 0.641943434956956 miRNAWalker2_validate -0.139797546065847 5.17117232636577e-06 NA NA NA hsa-miR-21-5p TNRC6B 1.51084295924322 1.54551622173259e-34 -0.188109276425255 0.0335580000177885 miRNAWalker2_validate;miRNATAP -0.167703483989802 1.3501746958449e-07 NA NA NA hsa-miR-21-5p TNS3 1.51084295924322 1.54551622173259e-34 -0.178816306653718 0.126735298257505 miRNAWalker2_validate -0.17568323830764 3.3969387411453e-05 NA NA NA hsa-miR-21-5p TOPORS 1.51084295924322 1.54551622173259e-34 -0.476181605025169 2.0539555781362e-07 miRNAWalker2_validate -0.26999537294657 1.24551741581356e-16 NA NA NA hsa-miR-21-5p TOR1AIP2 1.51084295924322 1.54551622173259e-34 -0.337836449293359 0.0591267564574758 mirMAP -0.298788114919202 3.98871752967024e-06 NA NA NA hsa-miR-21-5p TPRG1L 1.51084295924322 1.54551622173259e-34 -0.758883371513601 2.47046358234149e-10 miRNAWalker2_validate;MirTarget -0.319834211135076 1.78076309778345e-13 NA NA NA hsa-miR-21-5p TRIM2 1.51084295924322 1.54551622173259e-34 -0.669569001903942 0.000204730952595103 miRNAWalker2_validate;mirMAP;miRNATAP -0.360649471365021 3.33786514044469e-08 NA NA NA hsa-miR-21-3p TRIM66 -0.484219378716006 0.00299941098417659 0.807068904444835 4.08730410631591e-10 mirMAP -0.134736736582459 0.000585701672235426 NA NA NA hsa-miR-21-5p TRPM7 1.51084295924322 1.54551622173259e-34 -0.386704410514037 0.00191776856143715 miRNAWalker2_validate;miRNATAP -0.364353905234612 1.1645509151234e-16 NA NA NA hsa-miR-221-3p TSC22D2 1.12244756358612 3.48370308238012e-13 -1.12090763359319 2.22265538164126e-16 miRNAWalker2_validate -0.204958960929289 2.04978297616466e-06 NA NA NA hsa-miR-21-5p TSHZ1 1.51084295924322 1.54551622173259e-34 -0.489626961191658 7.53435228390986e-07 miRNATAP -0.312475230791863 3.84129485458031e-19 NA NA NA hsa-miR-21-5p TTC33 1.51084295924322 1.54551622173259e-34 -0.109909665894112 0.210986735501256 miRNAWalker2_validate -0.195027613607619 4.74386595104879e-10 NA NA NA hsa-miR-21-5p UBE2D3 1.51084295924322 1.54551622173259e-34 -0.546454726317387 9.53324910574043e-17 MirTarget;miRNATAP -0.171121255574583 1.36981728044781e-12 NA NA NA hsa-miR-21-5p UBE4A 1.51084295924322 1.54551622173259e-34 -0.18436367635478 0.00481429470133437 MirTarget;miRNATAP -0.134852775740646 8.06058947743651e-09 NA NA NA hsa-miR-21-5p UBR3 1.51084295924322 1.54551622173259e-34 -0.651263693121249 4.19615459779866e-12 miRNAWalker2_validate;MirTarget;miRNATAP -0.339040574154486 1.53829829139559e-24 NA NA NA hsa-miR-21-5p UBXN10 1.51084295924322 1.54551622173259e-34 -1.75060710944774 2.42078409774784e-06 mirMAP -0.749966978736364 2.92990620999404e-08 NA NA NA hsa-miR-21-5p UHMK1 1.51084295924322 1.54551622173259e-34 -0.803204254827771 0.00347508080027661 mirMAP -0.453633017143944 5.571326226094e-06 NA NA NA hsa-miR-21-5p USP15 1.51084295924322 1.54551622173259e-34 -0.501194395838042 8.03199763675317e-16 MirTarget -0.183025140623556 5.04387738895134e-16 NA NA NA hsa-miR-21-5p USP47 1.51084295924322 1.54551622173259e-34 -0.288927257048455 1.31798435018082e-05 miRNAWalker2_validate -0.192448823170205 1.94317660562691e-16 NA NA NA hsa-miR-21-5p VPS13A 1.51084295924322 1.54551622173259e-34 -0.258302170047732 0.0617206638390793 miRNAWalker2_validate -0.115758656468014 0.0218246546866006 NA NA NA hsa-miR-21-5p VPS36 1.51084295924322 1.54551622173259e-34 -0.567458160061146 1.90597492149183e-08 miRNAWalker2_validate -0.272758977873715 5.74486244863826e-14 NA NA NA hsa-miR-21-5p VSNL1 1.51084295924322 1.54551622173259e-34 -0.874873254541304 0.0199688194916894 MirTarget -0.326524166662513 0.0176627942750373 NA NA NA hsa-miR-21-5p WDR7 1.51084295924322 1.54551622173259e-34 -0.721226430814737 5.8107002873624e-15 miRNAWalker2_validate -0.337348716012021 8.71348629464671e-25 NA NA NA hsa-miR-21-5p WNK3 1.51084295924322 1.54551622173259e-34 -0.836532944570787 0.00785592148273515 miRNAWalker2_validate -0.876883933429564 3.74623998197249e-15 NA NA NA hsa-miR-221-3p WNK3 1.12244756358612 3.48370308238012e-13 -0.836532944570787 0.00785592148273515 miRNATAP -0.359498038872179 0.000199806844922679 NA NA NA hsa-miR-21-5p WWC2 1.51084295924322 1.54551622173259e-34 -0.895367958967769 3.36316136449853e-11 miRNAWalker2_validate -0.397341260935548 3.38337582715096e-16 NA NA NA hsa-miR-21-5p XPNPEP3 1.51084295924322 1.54551622173259e-34 -0.229940045643978 0.00504351402350879 mirMAP -0.124609379810193 2.69785828752746e-05 NA NA NA hsa-miR-21-5p YPEL2 1.51084295924322 1.54551622173259e-34 -1.00622202873194 2.21196225043249e-15 mirMAP -0.2748162339351 5.62347618311405e-09 NA NA NA hsa-miR-21-5p ZADH2 1.51084295924322 1.54551622173259e-34 -1.17032856012737 5.54900778507354e-29 miRNAWalker2_validate;mirMAP -0.449898962343669 1.55114134739767e-32 NA NA NA hsa-miR-221-3p ZADH2 1.12244756358612 3.48370308238012e-13 -1.17032856012737 5.54900778507354e-29 MirTarget -0.204256955835515 1.70495598867942e-09 NA NA NA hsa-miR-21-5p ZBTB20 1.51084295924322 1.54551622173259e-34 -0.721517199741877 6.05331504375829e-06 miRNAWalker2_validate -0.308921012280672 1.02102349879985e-07 NA NA NA hsa-miR-21-5p ZBTB38 1.51084295924322 1.54551622173259e-34 -0.322190916866726 0.022072727436213 miRNAWalker2_validate -0.135442175892406 0.00840003737850539 NA NA NA hsa-miR-221-3p ZC3H13 1.12244756358612 3.48370308238012e-13 -0.683664609759774 3.46943555156077e-07 MirTarget -0.141864841939699 0.000660440736530989 NA NA NA hsa-miR-21-5p ZDHHC17 1.51084295924322 1.54551622173259e-34 -0.16799106872775 0.0488926508637065 miRNATAP -0.128394985020913 3.10775657611721e-05 NA NA NA hsa-miR-221-3p ZEB2 1.12244756358612 3.48370308238012e-13 -1.50896689448163 2.21480558222559e-19 miRNAWalker2_validate;MirTarget;miRNATAP -0.182551774312563 0.000690586802131325 NA NA NA hsa-miR-21-5p ZFP36L2 1.51084295924322 1.54551622173259e-34 -0.368277506129601 0.000489675160959715 MirTarget -0.126203314684949 0.00107280663992073 NA NA NA hsa-miR-221-3p ZFPM2 1.12244756358612 3.48370308238012e-13 -2.22408950189141 1.94401967403455e-22 miRNATAP -0.46799720845663 1.13002039335422e-10 NA NA NA hsa-miR-21-3p ZHX3 -0.484219378716006 0.00299941098417659 -0.0728476988937654 0.495793402490393 mirMAP -0.101993260191739 0.00130225057418133 NA NA NA hsa-miR-21-5p ZNF24 1.51084295924322 1.54551622173259e-34 -0.418019959917853 3.16023417649809e-11 mirMAP -0.166170110277504 2.59599760704008e-13 NA NA NA hsa-miR-21-3p ZNF248 -0.484219378716006 0.00299941098417659 0.601489520563681 5.20265014741703e-12 MirTarget -0.120423940807891 5.00206398893248e-06 NA NA NA hsa-miR-21-5p ZNF326 1.51084295924322 1.54551622173259e-34 -0.426269705905494 6.21862215532185e-08 miRNAWalker2_validate -0.17477153056191 9.01092351909685e-10 NA NA NA hsa-miR-21-5p ZNF367 1.51084295924322 1.54551622173259e-34 -0.513348841626488 0.00175456475987379 miRNAWalker2_validate;miRNATAP -0.286148705844577 1.52740310844254e-06 NA NA NA hsa-miR-21-5p ZNF451 1.51084295924322 1.54551622173259e-34 0.00667997245057173 0.935667435375475 mirMAP -0.142196739648657 1.72736524516026e-06 NA NA NA hsa-miR-21-5p ZNF527 1.51084295924322 1.54551622173259e-34 0.260675782849676 0.00547326878026242 MirTarget -0.105578034047756 0.00200976037321645 NA NA NA hsa-miR-21-3p ZNF652 -0.484219378716006 0.00299941098417659 0.169367285673935 0.0353229494109345 MirTarget -0.121424732115483 2.78726964762337e-07 NA NA NA hsa-miR-21-5p ZNF654 1.51084295924322 1.54551622173259e-34 -0.621209689571205 1.69740975453914e-13 miRNATAP -0.284418544182187 3.24854923597832e-21 NA NA NA hsa-miR-21-5p ZNF667 1.51084295924322 1.54551622173259e-34 -0.675731521836621 0.0246747711488253 miRNAWalker2_validate -0.410646134854514 0.000176940975400381 NA NA NA hsa-miR-21-5p ZNF680 1.51084295924322 1.54551622173259e-34 -0.548304197900761 0.000339937850652107 MirTarget -0.407989154462753 7.3992267622587e-14 NA NA NA hsa-miR-21-3p ZNF772 -0.484219378716006 0.00299941098417659 -0.00107440076192367 0.994829945501399 mirMAP -0.259587769619618 9.97693192912258e-08 NA NA NA hsa-miR-21-3p ZNF780B -0.484219378716006 0.00299941098417659 0.0993812902607312 0.410868781515265 mirMAP -0.174758585548908 9.00966593581906e-07 NA NA NA hsa-miR-21-5p ZRANB1 1.51084295924322 1.54551622173259e-34 -0.533805690434926 1.19229356780518e-07 miRNAWalker2_validate -0.415103924712192 1.29253349161736e-33 NA NA NA hsa-miR-21-3p ZYG11B -0.484219378716006 0.00299941098417659 -0.715499327103507 8.21161432354526e-11 MirTarget -0.161735871024004 1.1285313171122e-06 NA NA NA hsa-miR-21-5p ZYG11B 1.51084295924322 1.54551622173259e-34 -0.715499327103507 8.21161432354526e-11 miRNAWalker2_validate;mirMAP -0.391557188035884 6.60996513323152e-24 NA NA NA