miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-125a-5p ATM -0.0833349424517387 0.325440200253607 -0.0447507082085021 0.439654845969846 miRanda -0.191891258396068 1.08085791159268e-10 NA NA NA hsa-miR-146b-5p ATM 0.441078059667297 0.000237619929889385 -0.0447507082085021 0.439654845969846 miRanda -0.139090363114708 3.02172223504569e-15 27602131 The role of microRNA 146b miR-146b in ATC remains to be elucidated; In order to characterize the role of miR-146b in ATC overexpression or interference of miR-146b was induced in ATC cell lines and cell proliferation and migration were evaluated; The potential targets of miR-146b were searched in the Gene Expression Omnibus database for ATC and matched non-tumor control samples; The expression level of potential targets was detected following overexpression or interference of miR-146b in ATC cell lines; In addition cell migration of ATC was also affected by miR-146b; During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis; In conclusion p21 may participate in the regulation of ATC cell proliferation by miR-146b cell migration;progression thyroid cancer hsa-miR-186-5p ATM 0.0606381894130505 0.514217717592464 -0.0447507082085021 0.439654845969846 mirMAP -0.123208669272272 4.47711357729956e-07 NA NA NA hsa-miR-18a-5p ATM 0.175320895022849 0.0732549187136286 -0.0447507082085021 0.439654845969846 miRNAWalker2_validate;miRTarBase;MirTarget -0.104121682157558 6.75084520132396e-07 23437304;25963391;23857602;23229340 MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression colorectal cancer;cervical and endocervical cancer;breast cancer;colon cancer hsa-miR-26b-5p ATM -0.0984536538762768 0.231803769574623 -0.0447507082085021 0.439654845969846 MirTarget -0.123652362350772 3.89261799231929e-05 NA NA NA hsa-miR-30b-5p ATM -0.0858101016322106 0.263243418521854 -0.0447507082085021 0.439654845969846 mirMAP -0.145142243457673 7.69838249932177e-06 25202329 However specific miRNAs are downregulated in ATC such as those of the miR-200 and miR-30 families which are important negative regulators of cell migration invasion and epithelial-to-mesenchymal transition EMT processes that are overactivated in ATC cell migration thyroid cancer hsa-miR-30c-5p ATM -0.0469114469943097 0.565934557464627 -0.0447507082085021 0.439654845969846 mirMAP -0.21095555767297 5.90764153731747e-11 NA NA NA hsa-miR-30d-3p ATM 0.0854049347792898 0.20691254801121 -0.0447507082085021 0.439654845969846 mirMAP -0.108465899695928 0.000445378990366815 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-30d-5p ATM -0.0607010868720046 0.471410043548794 -0.0447507082085021 0.439654845969846 mirMAP -0.1256777304023 0.000751902317966877 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-324-5p ATM 0.0183370482495953 0.842996782677271 -0.0447507082085021 0.439654845969846 miRanda -0.136864230463836 1.45860904388815e-09 NA NA NA hsa-miR-339-5p ATM 0.273253751377152 0.0185300170790638 -0.0447507082085021 0.439654845969846 miRanda -0.10715400072152 1.20256225486914e-09 NA NA NA hsa-miR-500a-5p ATM 0.0634452729978863 0.396072382601677 -0.0447507082085021 0.439654845969846 mirMAP -0.13339851803771 1.47786050929078e-06 NA NA NA hsa-miR-766-3p ATM -0.147747825439875 0.266658981351507 -0.0447507082085021 0.439654845969846 MirTarget -0.100027991553697 4.5702698390424e-11 NA NA NA hsa-miR-346 BAX -0.577786425963268 2.10883270131593e-09 0.353229927783273 4.30723325351116e-09 PITA;miRanda;miRNATAP -0.173817100628184 4.61458517988793e-13 NA NA NA hsa-miR-504-5p BAX -0.510726779625117 6.12122269907368e-05 0.353229927783273 4.30723325351116e-09 miRNAWalker2_validate -0.168060346140926 3.36340342595628e-20 NA NA NA hsa-miR-140-5p BID 0.065063507103468 0.380452509211773 -0.303077853524202 1.77086057276777e-05 miRanda -0.226072474033958 8.47902007941316e-09 NA NA NA hsa-miR-142-3p BID 0.558908907203977 1.37828834702416e-05 -0.303077853524202 1.77086057276777e-05 MirTarget;miRanda -0.209613625216468 5.20251278351838e-24 NA NA NA hsa-miR-149-5p BID -0.0535821592029952 0.657713892012548 -0.303077853524202 1.77086057276777e-05 miRNAWalker2_validate -0.175744339411029 3.58377936748149e-15 NA NA NA hsa-miR-17-5p BID 0.0975751369167082 0.20621110214601 -0.303077853524202 1.77086057276777e-05 TargetScan -0.150049651662491 0.000572947477601046 NA NA NA hsa-miR-199a-5p BID 0.174889610847968 0.0900027418912454 -0.303077853524202 1.77086057276777e-05 miRanda -0.180779053931333 4.77962650285497e-12 NA NA NA hsa-miR-199b-5p BID 0.436298593972862 0.00200225454383871 -0.303077853524202 1.77086057276777e-05 miRanda -0.170740349718571 1.69717072548974e-20 NA NA NA hsa-miR-335-3p BID 0.34540584335595 0.00043848660222294 -0.303077853524202 1.77086057276777e-05 MirTarget -0.187737200480552 2.35088015189847e-11 NA NA NA hsa-miR-543 BID -0.347737549779625 0.0157394585079683 -0.303077853524202 1.77086057276777e-05 miRanda -0.110677529644886 5.61425667539111e-09 NA NA NA hsa-let-7g-5p CASP3 -0.0253396334608382 0.742556489443668 0.262751412479442 2.56855295665157e-06 MirTarget;miRNATAP -0.129202676446113 0.000422033827716308 NA NA NA hsa-miR-101-3p CASP3 -0.132333931567244 0.241891922291381 0.262751412479442 2.56855295665157e-06 MirTarget -0.106383210575799 1.67996775990553e-05 NA NA NA hsa-miR-128-3p CASP3 -0.781932174412672 7.1812756321342e-10 0.262751412479442 2.56855295665157e-06 miRNAWalker2_validate -0.154501736884318 6.74484437136043e-18 NA NA NA hsa-miR-138-5p CASP3 -0.616087649920851 1.35200027885729e-05 0.262751412479442 2.56855295665157e-06 miRNAWalker2_validate;miRTarBase -0.113189968294872 3.60467356853659e-14 NA NA NA hsa-miR-139-5p CASP3 -0.647754756897633 2.15378307237163e-06 0.262751412479442 2.56855295665157e-06 miRanda -0.14903095658868 1.69348733053664e-21 NA NA NA hsa-miR-30d-5p CASP3 -0.0607010868720046 0.471410043548794 0.262751412479442 2.56855295665157e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.147143327631089 0.000150487575452898 NA NA NA hsa-miR-342-3p CASP3 -0.125683829770877 0.105810751640137 0.262751412479442 2.56855295665157e-06 miRanda -0.139797539988921 6.72797720817884e-06 NA NA NA hsa-miR-374b-5p CASP3 -0.210113949579608 0.0056866083818614 0.262751412479442 2.56855295665157e-06 mirMAP -0.148669291364588 3.58505826678344e-06 NA NA NA hsa-miR-7-1-3p CASP3 -0.0894475316162393 0.37322359785108 0.262751412479442 2.56855295665157e-06 MirTarget -0.104664105333427 1.16615180856721e-06 NA NA NA hsa-miR-105-5p CASP8 -0.567459149851677 8.20858944634795e-06 0.54677465094659 3.45072924958376e-10 MirTarget -0.327558183042417 2.59399847236527e-29 NA NA NA hsa-miR-129-5p CASP8 -0.567133990117126 0.00116152215679936 0.54677465094659 3.45072924958376e-10 miRanda -0.121355359480355 5.2366688422782e-08 NA NA NA hsa-miR-137 CASP8 -0.924849266740909 1.546832202049e-06 0.54677465094659 3.45072924958376e-10 miRanda -0.129351826753639 2.71139751772931e-11 NA NA NA hsa-miR-490-3p CASP8 -0.834268051506884 5.26203010743215e-06 0.54677465094659 3.45072924958376e-10 miRanda -0.119572674587039 1.12289344974493e-08 NA NA NA hsa-miR-125a-3p CASP9 0.183834985432376 0.106230647107917 -0.190390818122219 0.00860629060432951 miRanda -0.10922116203645 1.51947097140883e-05 NA NA NA hsa-miR-126-5p CASP9 0.177332403948664 0.0711675116243123 -0.190390818122219 0.00860629060432951 MirTarget -0.260533886267505 9.08731800375383e-19 NA NA NA hsa-miR-133a-3p CASP9 -0.335058908302964 0.0180402783121034 -0.190390818122219 0.00860629060432951 miRNAWalker2_validate;miRTarBase -0.126150713979561 1.5336707780324e-10 NA NA NA hsa-miR-140-5p CASP9 0.065063507103468 0.380452509211773 -0.190390818122219 0.00860629060432951 miRanda -0.198208812177151 1.81618262797927e-06 NA NA NA hsa-miR-15a-5p CASP9 0.142658985373611 0.0536219185009004 -0.190390818122219 0.00860629060432951 mirMAP -0.235687914842974 3.68148704667338e-07 NA NA NA hsa-miR-15b-5p CASP9 0.592863156692837 1.41971517354055e-10 -0.190390818122219 0.00860629060432951 mirMAP -0.194862381425862 1.11164072592137e-09 NA NA NA hsa-miR-16-5p CASP9 0.246024668671282 0.00346042804503716 -0.190390818122219 0.00860629060432951 mirMAP -0.203519004171527 3.05123518551225e-07 NA NA NA hsa-miR-193b-3p CASP9 0.0766333116224072 0.324723824453143 -0.190390818122219 0.00860629060432951 miRNAWalker2_validate -0.287691751817182 2.55461285152645e-14 NA NA NA hsa-miR-199a-3p CASP9 0.41226331871076 0.000497136154474528 -0.190390818122219 0.00860629060432951 mirMAP -0.203767948496481 7.25854614561419e-18 23319430 The techniques used were the MTT assay flow cytometry real-time PCR to assess miR-199a expression as also caspase-8 and caspase-9 activity in HepG2 cells treated with Propofol liver cancer hsa-miR-199b-3p CASP9 0.411848361704393 0.000517815011892379 -0.190390818122219 0.00860629060432951 mirMAP -0.202937922848071 8.40130462827375e-18 NA NA NA hsa-miR-342-5p CASP9 0.0383784541944436 0.544128449296127 -0.190390818122219 0.00860629060432951 MirTarget -0.172780651339529 0.000183272409998028 NA NA NA hsa-miR-424-5p CASP9 0.25140374049676 0.00709934939774983 -0.190390818122219 0.00860629060432951 mirMAP -0.121649518123937 0.000136932797700071 NA NA NA hsa-miR-450b-5p CASP9 0.270544879013992 0.00801537838499562 -0.190390818122219 0.00860629060432951 mirMAP -0.107405523905285 0.000148848993244956 NA NA NA hsa-miR-495-3p CASP9 -0.421982588962504 0.00504424038526901 -0.190390818122219 0.00860629060432951 mirMAP -0.112698137537794 1.81004622187323e-09 NA NA NA hsa-miR-548b-3p CASP9 0.340474544210108 0.00224491592019745 -0.190390818122219 0.00860629060432951 MirTarget -0.12601735851912 9.6237618924232e-07 NA NA NA hsa-miR-590-3p CASP9 0.312951515438396 0.000480455423843996 -0.190390818122219 0.00860629060432951 miRanda -0.109539366255286 0.000563304082980271 NA NA NA hsa-miR-139-5p CCNB1 -0.647754756897633 2.15378307237163e-06 0.84976221863878 1.34547967203245e-21 miRanda -0.158522988130583 1.297334982042e-07 NA NA NA hsa-miR-339-5p CCNB3 0.273253751377152 0.0185300170790638 -0.234870852552629 0.00599558926982139 miRanda -0.176872777316358 3.1676352111193e-08 NA NA NA hsa-miR-361-5p CCNB3 0.0107519600643302 0.882230614095565 -0.234870852552629 0.00599558926982139 miRanda -0.221101186108731 0.000565419134065904 NA NA NA hsa-miR-590-3p CCNB3 0.312951515438396 0.000480455423843996 -0.234870852552629 0.00599558926982139 miRanda -0.182806767559893 1.10227582601784e-05 NA NA NA hsa-let-7e-5p CCND1 0.0471189209570344 0.657255455444655 0.517304800763483 1.04046594178888e-06 miRTarBase;miRNATAP -0.228941875441154 1.78800254985128e-06 NA NA NA hsa-let-7i-5p CCND1 0.0952853587923244 0.192120688957249 0.517304800763483 1.04046594178888e-06 miRNATAP -0.236343082962889 0.00183350614645 NA NA NA hsa-miR-1266-5p CCND1 0.313515127917259 0.0138664194727392 0.517304800763483 1.04046594178888e-06 MirTarget -0.112027246009442 0.00108554865163972 NA NA NA hsa-miR-135a-5p CCND1 -0.122734612943241 0.196985964641886 0.517304800763483 1.04046594178888e-06 mirMAP -0.247492714890572 1.3238406350361e-07 NA NA NA hsa-miR-23b-3p CCND1 0.0476610245860094 0.630277683372344 0.517304800763483 1.04046594178888e-06 miRNATAP -0.279243855267394 9.57342364736838e-09 NA NA NA hsa-miR-26b-5p CCND1 -0.0984536538762768 0.231803769574623 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate -0.171067179820866 0.00692173397836684 NA NA NA hsa-miR-29a-3p CCND1 -0.224140851576083 0.0349516103550236 0.517304800763483 1.04046594178888e-06 mirMAP -0.23358540897967 1.12603356998685e-05 NA NA NA hsa-miR-29b-3p CCND1 -0.178522712044497 0.0856106432165465 0.517304800763483 1.04046594178888e-06 mirMAP -0.30608004962895 3.25721913642118e-11 NA NA NA hsa-miR-29c-3p CCND1 -0.372180390387259 0.00202634431973208 0.517304800763483 1.04046594178888e-06 mirMAP -0.263497298429692 1.34805822955252e-11 NA NA NA hsa-miR-3065-5p CCND1 -0.262608140481507 0.0414570974148034 0.517304800763483 1.04046594178888e-06 mirMAP -0.105747126080182 0.00178155205723976 NA NA NA hsa-miR-330-3p CCND1 -0.427388106938151 0.00300489284414266 0.517304800763483 1.04046594178888e-06 mirMAP -0.156757729272097 1.33987981854083e-07 NA NA NA hsa-miR-338-3p CCND1 -0.39963704679384 0.0034410643220691 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase;miRanda -0.138060683824931 7.24806702911648e-05 NA NA NA hsa-miR-34a-5p CCND1 0.143472919835524 0.211283228567057 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.176667405034615 4.78965946188406e-06 25792709;21399894 This inhibition of proliferation was associated with a decrease in cyclin D1 levels orchestrated principally by HNF-4α a target of miR-34a considered to act as a tumour suppressor in the liver;Quantitative PCR and western analysis confirmed decreased expression of two genes BCL-2 and CCND1 in docetaxel-resistant cells which are both targeted by miR-34a ; liver cancer;breast cancer hsa-miR-365a-3p CCND1 -0.184222766207919 0.0292663235724171 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase -0.195991913128339 0.000198680829195249 NA NA NA hsa-miR-425-5p CCND1 -0.0412837711174561 0.682284485592858 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate -0.134644465621285 0.00325561137510874 NA NA NA hsa-miR-490-3p CCND1 -0.834268051506884 5.26203010743215e-06 0.517304800763483 1.04046594178888e-06 miRanda -0.131117175699242 2.90415525218072e-08 24440705 MicroRNA 490 3p inhibits proliferation of A549 lung cancer cells by targeting CCND1; We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1 which plays a key role in G1/S phase transition; In addition the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3'UTR lung cancer hsa-miR-495-3p CCND1 -0.421982588962504 0.00504424038526901 0.517304800763483 1.04046594178888e-06 MirTarget -0.136090131725584 2.1911780731285e-06 NA NA NA hsa-miR-7-1-3p CCND1 -0.0894475316162393 0.37322359785108 0.517304800763483 1.04046594178888e-06 mirMAP -0.241924898170475 2.08913227927897e-08 NA NA NA hsa-miR-769-3p CCND1 -0.287263649023213 0.018145224330845 0.517304800763483 1.04046594178888e-06 mirMAP -0.276564457533319 1.97356475814615e-15 NA NA NA hsa-miR-885-5p CCND1 -0.423614695846868 1.14213289528402e-05 0.517304800763483 1.04046594178888e-06 miRNATAP -0.292419966156513 1.08197956283879e-10 NA NA NA hsa-let-7b-3p CCND2 -0.00495618733298731 0.952288980686967 0.278072058804804 0.00230941164668614 mirMAP -0.124812140552693 0.00599796857804289 NA NA NA hsa-let-7f-1-3p CCND2 0.110713086694053 0.21484524300518 0.278072058804804 0.00230941164668614 mirMAP -0.122537465829283 0.00233902672067921 NA NA NA hsa-let-7g-5p CCND2 -0.0253396334608382 0.742556489443668 0.278072058804804 0.00230941164668614 miRNATAP -0.196434601966773 0.0012996932102575 NA NA NA hsa-let-7i-5p CCND2 0.0952853587923244 0.192120688957249 0.278072058804804 0.00230941164668614 miRNATAP -0.162156277541767 0.00988534673137717 NA NA NA hsa-miR-129-5p CCND2 -0.567133990117126 0.00116152215679936 0.278072058804804 0.00230941164668614 mirMAP -0.105272286982926 4.44505393807218e-07 NA NA NA hsa-miR-145-3p CCND2 0.110543172922298 0.294150620210019 0.278072058804804 0.00230941164668614 mirMAP -0.149944712694934 9.82587587919985e-06 NA NA NA hsa-miR-145-5p CCND2 0.108948399943015 0.310032352776693 0.278072058804804 0.00230941164668614 miRNATAP -0.155654659327631 1.04593167784463e-05 NA NA NA hsa-miR-146b-3p CCND2 0.283486177568242 0.0118918188864879 0.278072058804804 0.00230941164668614 MirTarget;PITA;miRNATAP -0.117766891479947 0.000283548163350027 NA NA NA hsa-miR-150-5p CCND2 0.0645271721519611 0.513295658828718 0.278072058804804 0.00230941164668614 mirMAP -0.149451068560082 7.57763104304709e-05 NA NA NA hsa-miR-151a-3p CCND2 0.0336074122085215 0.726834677736803 0.278072058804804 0.00230941164668614 mirMAP -0.12420907434582 0.00497911559673839 NA NA NA hsa-miR-185-5p CCND2 -0.0767432095218297 0.404113540276816 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.183398276514872 8.3630357382723e-06 NA NA NA hsa-miR-186-5p CCND2 0.0606381894130505 0.514217717592464 0.278072058804804 0.00230941164668614 mirMAP;miRNATAP -0.133720175872038 0.00173369326890833 NA NA NA hsa-miR-191-5p CCND2 0.0973986771912738 0.220078671774056 0.278072058804804 0.00230941164668614 MirTarget -0.20451499794437 0.00023723661594718 NA NA NA hsa-miR-26a-5p CCND2 0.033823776747294 0.726610414342369 0.278072058804804 0.00230941164668614 miRNAWalker2_validate;miRTarBase;mirMAP;miRNATAP -0.122521188171157 0.00669669430395561 NA NA NA hsa-miR-26b-5p CCND2 -0.0984536538762768 0.231803769574623 0.278072058804804 0.00230941164668614 mirMAP;miRNATAP -0.21765809461179 3.01296451472833e-05 NA NA NA hsa-miR-28-5p CCND2 0.104003572105781 0.153594705579534 0.278072058804804 0.00230941164668614 miRanda -0.151040812510917 0.00458703218164268 NA NA NA hsa-miR-29a-3p CCND2 -0.224140851576083 0.0349516103550236 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.199711571525784 5.61968185759315e-06 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29a-5p CCND2 -0.0697727833812678 0.350621682973119 0.278072058804804 0.00230941164668614 mirMAP -0.166759307844904 0.000688470083956136 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29b-3p CCND2 -0.178522712044497 0.0856106432165465 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.202303651104096 1.45292987304036e-07 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29c-3p CCND2 -0.372180390387259 0.00202634431973208 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.239386359038839 8.64180673949139e-14 NA NA NA hsa-miR-30d-3p CCND2 0.0854049347792898 0.20691254801121 0.278072058804804 0.00230941164668614 mirMAP -0.155355754639963 0.00382748112364014 NA NA NA hsa-miR-30e-3p CCND2 0.00173769765874887 0.984965501547409 0.278072058804804 0.00230941164668614 mirMAP -0.268522462794922 4.02496537819957e-07 NA NA NA hsa-miR-324-3p CCND2 -0.0103367134443655 0.904494071318044 0.278072058804804 0.00230941164668614 miRNAWalker2_validate -0.184828101121747 1.93642919504512e-05 NA NA NA hsa-miR-331-5p CCND2 0.00538076980695534 0.942833685963252 0.278072058804804 0.00230941164668614 miRNATAP -0.168360380366874 0.000470859110297375 NA NA NA hsa-miR-33a-3p CCND2 -0.158366376619191 0.0991512910387226 0.278072058804804 0.00230941164668614 MirTarget -0.10197096016646 0.00681801603426165 NA NA NA hsa-miR-3622a-3p CCND2 0.0389203848593471 0.75605812962459 0.278072058804804 0.00230941164668614 mirMAP -0.125163354365051 1.78891207109425e-05 NA NA NA hsa-miR-488-3p CCND2 -0.257955761012568 0.00280795162806256 0.278072058804804 0.00230941164668614 mirMAP -0.183392273105331 1.52514393662224e-05 NA NA NA hsa-miR-488-5p CCND2 -0.385755602369049 7.99431673275965e-08 0.278072058804804 0.00230941164668614 mirMAP -0.153852506035559 0.00315722783501446 NA NA NA hsa-miR-500a-5p CCND2 0.0634452729978863 0.396072382601677 0.278072058804804 0.00230941164668614 mirMAP -0.155381019814906 0.0013241335866013 NA NA NA hsa-miR-501-5p CCND2 0.0878951859817452 0.309663377222443 0.278072058804804 0.00230941164668614 PITA;mirMAP;miRNATAP -0.110941849752897 0.00801840144207187 NA NA NA hsa-miR-660-5p CCND2 -0.138989893523975 0.0945491063873448 0.278072058804804 0.00230941164668614 mirMAP -0.238701834193771 2.94573193719288e-07 NA NA NA hsa-miR-7-1-3p CCND2 -0.0894475316162393 0.37322359785108 0.278072058804804 0.00230941164668614 mirMAP -0.150925481137646 2.69591030726187e-05 NA NA NA hsa-miR-192-5p CCNE1 -0.0426540615456616 0.621616927627554 0.253536339251842 6.27968052141139e-05 miRNAWalker2_validate -0.134085089321587 0.000290914914800468 NA NA NA hsa-miR-195-5p CCNE1 -0.00728332132631593 0.92969850577609 0.253536339251842 6.27968052141139e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.187859421689644 1.50466938767298e-07 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-497-5p CCNE1 -0.000783764887886562 0.991703566618871 0.253536339251842 6.27968052141139e-05 MirTarget;miRNATAP -0.107990353660474 0.00593270549342151 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-30a-5p CCNE2 -0.062942936611238 0.513851954700252 0.318474669342545 0.000263822187487161 miRNATAP -0.404174405891182 3.32123969313609e-10 NA NA NA hsa-miR-30d-5p CCNE2 -0.0607010868720046 0.471410043548794 0.318474669342545 0.000263822187487161 miRNATAP -0.248704351406826 0.000276173784789898 25843294 MicroRNA 30d 5p inhibits tumour cell proliferation and motility by directly targeting CCNE2 in non small cell lung cancer; In addition the re-introduction of CCNE2 expression antagonised the inhibitory effects of miR-30d-5p on the capacity of NSCLC cells for proliferation and motility motility lung squamous cell cancer hsa-miR-9-5p CCNG1 -0.125377971246422 0.269896477802284 0.0934380765860343 0.0537872647490795 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.227763946954994 2.4450745494354e-10 26152689 CCNG1 validated as a direct target of miR-9 mediates paclitaxel resistance; Methylation-associated miR-9 down-regulation is probably one of the key mechanisms for paclitaxel resistance in EOC cells via targeting CCNG1 drug resistance ovarian cancer hsa-let-7a-2-3p CCNG2 0.295235553592458 0.00290654476250609 -0.119917009119163 0.0350459315966934 MirTarget -0.102756897159306 2.34051153731279e-06 NA NA NA hsa-miR-135a-5p CCNG2 -0.122734612943241 0.196985964641886 -0.119917009119163 0.0350459315966934 MirTarget;miRNATAP -0.135256775822502 4.29999914255432e-09 NA NA NA hsa-miR-21-5p CCNG2 1.0504925175311 2.58081295984106e-11 -0.119917009119163 0.0350459315966934 mirMAP -0.132686298591375 2.54448310697497e-19 NA NA NA hsa-miR-26b-5p CCNG2 -0.0984536538762768 0.231803769574623 -0.119917009119163 0.0350459315966934 mirMAP -0.150925319257971 1.0846980549679e-06 NA NA NA hsa-miR-28-5p CCNG2 0.104003572105781 0.153594705579534 -0.119917009119163 0.0350459315966934 miRanda -0.154451001265378 9.23001217026964e-07 NA NA NA hsa-miR-3065-3p CCNG2 -0.261348288537884 0.0343753235107924 -0.119917009119163 0.0350459315966934 MirTarget;miRNATAP -0.159856607078787 7.4245383375086e-20 NA NA NA hsa-miR-3065-5p CCNG2 -0.262608140481507 0.0414570974148034 -0.119917009119163 0.0350459315966934 MirTarget;mirMAP -0.137559231214808 1.91685241123173e-17 NA NA NA hsa-miR-320c CCNG2 0.343259124189966 0.000248338401291051 -0.119917009119163 0.0350459315966934 mirMAP -0.122844133540121 1.28310756711071e-07 NA NA NA hsa-miR-374a-5p CCNG2 0.0121037060909277 0.865417963345881 -0.119917009119163 0.0350459315966934 mirMAP -0.145466578668616 6.2850265782515e-06 NA NA NA hsa-miR-576-5p CCNG2 0.106482276040047 0.194379152564166 -0.119917009119163 0.0350459315966934 mirMAP -0.165633288080333 2.15789259244248e-10 NA NA NA hsa-miR-590-3p CCNG2 0.312951515438396 0.000480455423843996 -0.119917009119163 0.0350459315966934 miRanda;mirMAP -0.1216811306551 2.75076981908578e-07 NA NA NA hsa-miR-590-5p CCNG2 0.16946027035949 0.0386004382081678 -0.119917009119163 0.0350459315966934 mirMAP -0.112950782739825 1.79478142014273e-05 NA NA NA hsa-miR-664a-3p CCNG2 0.0413918462467198 0.611334357897022 -0.119917009119163 0.0350459315966934 MirTarget;mirMAP -0.121244150088071 6.52892229429524e-06 NA NA NA hsa-miR-708-3p CCNG2 0.00458267260363954 0.962455132950708 -0.119917009119163 0.0350459315966934 MirTarget -0.172711831931792 7.70766647502576e-13 NA NA NA hsa-miR-93-5p CCNG2 0.139766237971122 0.101057320076629 -0.119917009119163 0.0350459315966934 miRNAWalker2_validate;MirTarget;miRNATAP -0.107774382424247 0.00549563203353949 NA NA NA hsa-miR-362-3p CD82 0.032375035235532 0.736465332563774 -0.0998124614282885 0.173309927350168 miRanda -0.100254810063697 0.000473505792469159 25652145 Anti miR 362 3p Inhibits Migration and Invasion of Human Gastric Cancer Cells by Its Target CD82; Next we analyzed the level of miR-362-3p expression and CD82 in different differentiated GC cells compared with a normal gastric mucosa cell by RT-PCR and Western blot; Dual-luciferase reporter assay and Western blot confirmed a direct interaction between miR-362-3p and CD82 3'UTR; After miR-362-3p and CD82 were silenced in GC cells we compared the transfected GC cells migration and invasion capacity by transwell assay; Western blot was used to detect the impact of CD82 and miR-362-3p on epithelial-to-mesenchymal transition markers in treated GC cells; Level of miR-362-3p expression was much higher in GC cells than in normal gastric mucosa cell and miR-362-3p expression negatively correlated with CD82 mRNA expression in these cell lines; Furthermore miR-362-3p expression induced corrected GC cell metastasis capacity by suppression of CD82 expression; This study illuminated that downregulation of miR-362-3p along with the upregulation of CD82 in GC cells resulted in the inhibition of GC migration and invasion; Thus our results suggested that miR-362-3p or CD82 can be exploited as a new potential target for control of GC in the future metastasis gastric cancer hsa-miR-103a-3p CDK2 -0.147443193527362 0.128400914654149 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate -0.244529342446133 9.50719675121511e-05 NA NA NA hsa-miR-124-3p CDK2 -0.506026514549291 0.0078056370358648 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate;miRTarBase -0.168041487619791 1.69965544728722e-19 NA NA NA hsa-miR-490-3p CDK2 -0.834268051506884 5.26203010743215e-06 0.813366814153226 5.60398748524507e-23 miRanda -0.197884061603562 5.32644876787653e-25 NA NA NA hsa-miR-495-3p CDK2 -0.421982588962504 0.00504424038526901 0.813366814153226 5.60398748524507e-23 mirMAP -0.162294675651709 1.05494965345435e-11 NA NA NA hsa-miR-7-1-3p CDK2 -0.0894475316162393 0.37322359785108 0.813366814153226 5.60398748524507e-23 mirMAP -0.215889061968793 2.42828507111814e-09 NA NA NA hsa-miR-885-5p CDK2 -0.423614695846868 1.14213289528402e-05 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate;miRTarBase -0.319170723509576 1.73805882382479e-17 NA NA NA hsa-miR-124-3p CDK4 -0.506026514549291 0.0078056370358648 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.149095153225998 7.48747415901698e-17 25348738;25731732;27323123 MiR 124 retards bladder cancer growth by directly targeting CDK4; In order to investigate the physiological role of miR-124 in bladder cancer target genes of miR-124 were predicted by the TargetScan software and cyclin-dependent kinase CDK4 which has been implicated as a regulator of cell cycle was chosen for further study; MiR-124 could significantly repress CDK4 expression by targeting its binding site in the 3'UTR of CDK4 in vitro; In both bladder cancer cell lines and tissues the expression of miR-124 was significantly down-regulated while CDK4 expression was up-regulated; And the expression of miR-124 and CDK4 showed an obvious inverse correlation in these xenograft tissues which was also observed in human bladder cancer tissue samples; Taken together our results strongly suggest that miR-124 can arrest cell cycle and restrain the growth of bladder cancer by targeting CDK4 directly;MiR 124 inhibits cell proliferation in breast cancer through downregulation of CDK4; We identified and confirmed that cyclin-dependent kinase 4 CDK4 was a direct target of miR-124; Overexpression of miR-124 suppressed CDK4 protein expression and attenuated cell viability proliferation and cell cycle progression in MCF-7 and MDA-MB-435S breast cancer cells in vitro; Overexpression of CDK4 partially rescued the inhibitory effect of miR-124 in the breast cancer cells; Our results demonstrate that miR-124 functions as a growth-suppressive miRNA and plays an important role in inhibiting tumorigenesis by targeting CDK4;miR 124 radiosensitizes human esophageal cancer cell TE 1 by targeting CDK4; Finally we identified that CDK4 is a direct target of miR-124 in TE-1 cells using target prediction algorithms and a luciferase reporter assay; Moreover western blot assay confirmed that CDK4 was downregulated during miR-124 transfection ;progression;tumorigenesis; bladder cancer;breast cancer;esophageal cancer hsa-miR-145-5p CDK4 0.108948399943015 0.310032352776693 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.175782720088333 2.61540642411898e-07 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-24-3p CDK4 0.133257261462871 0.212960921400853 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.144306679535031 6.44290009253847e-05 NA NA NA hsa-miR-34a-5p CDK4 0.143472919835524 0.211283228567057 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.118448417522733 0.000139347458480003 25789847 Real-time PCR and western blot analysis of extracted RNA and total protein revealed artemsinin and artesunate increased miR-34a expression in a dose-dependent manner correlating with down-regulation of the miR-34a target gene CDK4; Phytochemical treatments inhibited the luciferase activity of a construct containing the wild-type 3'UTR of CDK4 but not those with a mutated miR-34a binding site whereas transfection of miR-34a inhibitors ablated the phytochemical mediated down-regulation of CDK4 and induction of cell cycle arrest breast cancer hsa-miR-766-3p CDK4 -0.147747825439875 0.266658981351507 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate -0.211336637770052 5.92406894308187e-17 NA NA NA hsa-miR-101-3p CDK6 -0.132333931567244 0.241891922291381 0.514594252893836 5.86679673505214e-06 mirMAP -0.214050983778852 0.000115517087379797 NA NA NA hsa-miR-1179 CDK6 -0.117056451908751 0.310989525634765 0.514594252893836 5.86679673505214e-06 PITA;miRNATAP -0.142889521672303 0.000881663691628466 NA NA NA hsa-miR-1224-5p CDK6 -0.53941068839383 0.00201058335769201 0.514594252893836 5.86679673505214e-06 miRNATAP -0.101570856158794 0.000240685196252074 NA NA NA hsa-miR-129-5p CDK6 -0.567133990117126 0.00116152215679936 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate -0.103420012068894 0.00024955678235129 24055727 Interestingly we showed that cyclin dependent kinase 6 CDK6 a cell cycle-associated protein involved in G1-S transition was a target of miR-129 gastric cancer hsa-miR-137 CDK6 -0.924849266740909 1.546832202049e-06 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.100134233590921 5.13631857844561e-05 18577219;25342326;23178712;21051724 Transfection of microRNA-124 or microRNA-137 also induced G1 cell cycle arrest in U251 and SF6969 glioblastoma multiforme cells which was associated with decreased expression of cyclin-dependent kinase 6 and phosphorylated retinoblastoma pSer 807/811 proteins;Bioinformatics prediction and luciferase reporter assay revealed CDK6 as a target gene through which miR-137 exerted an inhibitory function;miR 137 inhibits the proliferation of lung cancer cells by targeting Cdc42 and Cdk6; Ectopic expression of miR-137 in lung cancer cells significantly downregulated Cdc42 Cdk6 and induced G1 cell cycle arrest leading to a significant decrease in cell growth in vivo and in vitro; Further both Cdc42 and Cdk6 were confirmed as targets of miR-137;The results showed that miR-137 can act as a tumor suppressor in uveal melanoma cell proliferation through downregulation of the targets MITF and CDK6 miR-137 may be epigenetically silenced during uveal melanoma tumorigenesis ;;;tumorigenesis glioblastoma;gastric cancer;lung cancer;melanoma hsa-miR-140-3p CDK6 -0.123864258867705 0.14796686619449 0.514594252893836 5.86679673505214e-06 miRNATAP -0.26390527008378 0.000421888747453862 NA NA NA hsa-miR-29a-3p CDK6 -0.224140851576083 0.0349516103550236 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.295012706425205 5.99888048504157e-07 22493297;23245396;20086245 In addition a target of miR-29a cyclin-dependent kinase 6 gene and a target of miR-142-3p TGF-β-activated kinase 1/MAP3K7 binding protein 2 gene are involved in the regulation of both monocytic and granulocytic differentiation;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL differentiation;; acute myeloid leukemia;melanoma;lymphoma hsa-miR-29b-3p CDK6 -0.178522712044497 0.0856106432165465 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.269230638368595 2.03842569918004e-07 26180082;23245396;25472644;23591808;27230400;20086245 Knockdown of NTSR1 increased the expression of miR-29b-1 and miR-129-3p which were responsible for the decreased CDK6 expression;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;Moreover miR-29b inhibited the expression of MCL1 and CDK6;Here we have identified the oncogene cyclin-dependent protein kinase 6 CDK6 as a direct target of miR-29b in lung cancer;MiR 29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6; In this study we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods; We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells; Furthermore we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues; The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL ;;;;; glioblastoma;melanoma;colorectal cancer;lung cancer;sarcoma;lymphoma hsa-miR-29c-3p CDK6 -0.372180390387259 0.00202634431973208 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.208117488470636 2.11482006457337e-06 26396669 Furthermore through qPCR and Western blot assays confirmed that overexpression of miR-29c reduced CDK6 mRNA and protein levels; miR-29c could inhibit the proliferation migration and invasion of bladder cancer cells via regulating CDK6 bladder cancer hsa-miR-30a-5p CDK6 -0.062942936611238 0.513851954700252 0.514594252893836 5.86679673505214e-06 mirMAP -0.216479485176302 0.00926959328929756 NA NA NA hsa-miR-30b-5p CDK6 -0.0858101016322106 0.263243418521854 0.514594252893836 5.86679673505214e-06 mirMAP -0.264336978353372 0.000515451713470756 NA NA NA hsa-miR-30d-3p CDK6 0.0854049347792898 0.20691254801121 0.514594252893836 5.86679673505214e-06 mirMAP -0.200436058332701 0.00560114486149501 NA NA NA hsa-miR-30d-5p CDK6 -0.0607010868720046 0.471410043548794 0.514594252893836 5.86679673505214e-06 mirMAP -0.300718358106314 0.000558788158739809 NA NA NA hsa-miR-338-3p CDK6 -0.39963704679384 0.0034410643220691 0.514594252893836 5.86679673505214e-06 mirMAP -0.123780088353193 0.00144999386822314 NA NA NA hsa-miR-362-5p CDK6 -0.0730185557229279 0.425572997292224 0.514594252893836 5.86679673505214e-06 mirMAP -0.16543927930901 0.0021247110211983 NA NA NA hsa-miR-488-5p CDK6 -0.385755602369049 7.99431673275965e-08 0.514594252893836 5.86679673505214e-06 mirMAP -0.265214262784328 0.000150929371106913 NA NA NA hsa-miR-491-3p CDK6 -0.523374665885358 1.93479565404951e-05 0.514594252893836 5.86679673505214e-06 PITA;miRNATAP -0.14151833133198 0.000308714771991301 NA NA NA hsa-miR-660-5p CDK6 -0.138989893523975 0.0945491063873448 0.514594252893836 5.86679673505214e-06 mirMAP -0.206589176560963 0.00107106139495866 NA NA NA hsa-miR-7-1-3p CDK6 -0.0894475316162393 0.37322359785108 0.514594252893836 5.86679673505214e-06 mirMAP -0.161897757484807 0.000856176984301135 NA NA NA hsa-miR-767-5p CDK6 -0.551653504513145 2.61109058244854e-05 0.514594252893836 5.86679673505214e-06 mirMAP -0.218990178100663 3.12583485290879e-09 NA NA NA hsa-miR-885-5p CDK6 -0.423614695846868 1.14213289528402e-05 0.514594252893836 5.86679673505214e-06 mirMAP -0.261342311783077 2.73713377344836e-07 NA NA NA hsa-miR-935 CDK6 -0.277848050075718 0.02166231531395 0.514594252893836 5.86679673505214e-06 mirMAP -0.11716238994515 0.00413414436299781 NA NA NA hsa-let-7c-5p CDKN1A -0.04295851886768 0.676052842748691 0.427121917792729 0.00123136007948734 MirTarget -0.323059985687549 1.83340518666355e-05 NA NA NA hsa-let-7i-5p CDKN1A 0.0952853587923244 0.192120688957249 0.427121917792729 0.00123136007948734 MirTarget -0.290880872793201 0.00310217886465504 NA NA NA hsa-miR-106b-5p CDKN1A 0.167096183547615 0.0497544396383451 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.354916178485011 1.93698403552949e-06 NA NA NA hsa-miR-125a-5p CDKN1A -0.0833349424517387 0.325440200253607 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase -0.417635698065873 2.90152784450923e-07 NA NA NA hsa-miR-129-2-3p CDKN1A -0.6175132197013 0.000561573620904867 0.427121917792729 0.00123136007948734 mirMAP -0.132493112138164 2.17805696270136e-05 NA NA NA hsa-miR-17-5p CDKN1A 0.0975751369167082 0.20621110214601 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.455125254344935 4.24790487714932e-07 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-455-3p CDKN1A 0.512701816402104 0.000459755983280155 0.427121917792729 0.00123136007948734 MirTarget -0.108213069650159 0.00527030056074479 NA NA NA hsa-miR-491-5p CDKN1A -0.749881113074983 8.22859263179194e-09 0.427121917792729 0.00123136007948734 miRanda -0.187701364863274 8.68850198681925e-06 NA NA NA hsa-miR-505-5p CDKN1A 0.0973008785018434 0.183209581426791 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;MirTarget -0.381395926281218 9.16666243828523e-07 NA NA NA hsa-miR-708-5p CDKN1A -0.00936519718301376 0.919857671524527 0.427121917792729 0.00123136007948734 MirTarget -0.340713510326019 2.02954343368862e-07 NA NA NA hsa-miR-93-5p CDKN1A 0.139766237971122 0.101057320076629 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.407487489770916 6.20600753969256e-05 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-98-5p CDKN1A -0.0806971683871618 0.251777941211683 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;MirTarget -0.306627890186986 0.000734149322136236 NA NA NA hsa-let-7g-5p CDKN2A -0.0253396334608382 0.742556489443668 0.0187886573712563 0.903922340142693 miRNAWalker2_validate;miRTarBase -0.34824822585108 0.00265574549691866 NA NA NA hsa-miR-124-3p CDKN2A -0.506026514549291 0.0078056370358648 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.250164816877691 1.31566252867206e-12 NA NA NA hsa-miR-125a-5p CDKN2A -0.0833349424517387 0.325440200253607 0.0187886573712563 0.903922340142693 miRanda -0.370077543367592 0.000189260563152586 NA NA NA hsa-miR-128-3p CDKN2A -0.781932174412672 7.1812756321342e-10 0.0187886573712563 0.903922340142693 MirTarget -0.262360804552711 6.19986805514199e-06 NA NA NA hsa-miR-155-5p CDKN2A 0.969837380981085 5.86551495710056e-15 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.18750964292148 0.000446932476411726 NA NA NA hsa-miR-16-5p CDKN2A 0.246024668671282 0.00346042804503716 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.399970122623873 2.77212055657459e-05 NA NA NA hsa-miR-192-5p CDKN2A -0.0426540615456616 0.621616927627554 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.355895513978692 0.000105711706086703 NA NA NA hsa-miR-24-3p CDKN2A 0.133257261462871 0.212960921400853 0.0187886573712563 0.903922340142693 miRNAWalker2_validate;miRTarBase -0.286694703652825 4.87185115774767e-05 26847530;22336108 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated; p27 and p16 were confirmed as targets of miR-24 in prostate cancer cells and a significant inverse correlation between miR-24 and p27 was revealed in clinical prostatectomy specimens; These findings provide evidence that miR-24 has a tumor suppressor role in prostate cancer and also targets p27 and p16 in prostate cancer cells;To study p14ARF biogenesis retinoblastoma cells were treated with the proteasome inhibitor MG132 and siRNA against miR-24; miR-24 a microRNA that represses p14ARF expression is expressed in retinoblastoma cell lines and correlates with lower protein expression when compared to other cell lines with high p14ARF mRNA; Transient over-expression of siRNA against miR-24 led to elevated p14ARF protein in retinoblastoma cells; p14ARF protein levels were restored without change in mRNA abundance upon miR-24 inhibition suggesting that miR-24 could functionally repress expression effectively blocking p53 tumor surveillance ; prostate cancer;retinoblastoma hsa-miR-34a-5p CDKN2A 0.143472919835524 0.211283228567057 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.275930017996842 5.30358193376552e-06 25862914;26734589 MiR-34a tumor levels significantly correlated with oropharyngeal origin p=0.0284 and p16 positivity p=0.0218;The Significance of miR 34a Expression in Endometrial Carcinogenesis: Correlation With Expression of p16 and Ki 67 Proteins in Endometrial Cancers; This study was undertaken to analyze miR-34a expression in simple endometrial hyperplasia and endometrial cancer and to evaluate the relationship between expression of miR-34a and p16 and Ki-67 proteins in endometrial cancers; These were analyzed for miR-34a expression by quantitative real-time PCR and the expression of p16 and Ki-67 proteins in endometrial cancers was evaluated by immunohistochemistry; Although not statistically significant the frequency of p16 and Ki-67 overexpression tended to be lower in the cases with miR-34a underexpression than in cases with miR-34a overexpression ;tumorigenesis head and neck cancer;endometrial cancer hsa-miR-365a-3p CDKN2A -0.184222766207919 0.0292663235724171 0.0187886573712563 0.903922340142693 MirTarget -0.214641593907008 0.00965027186755129 NA NA NA hsa-miR-455-3p CDKN2A 0.512701816402104 0.000459755983280155 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.168237751538781 0.000328290439616957 NA NA NA hsa-miR-671-5p CDKN2A 0.379677704170736 0.000129391032677459 0.0187886573712563 0.903922340142693 PITA -0.335505749888256 7.04085752831714e-07 NA NA NA hsa-miR-940 CDKN2A -0.0183807673806575 0.881747775878136 0.0187886573712563 0.903922340142693 MirTarget -0.411718461242004 1.98167555161455e-14 NA NA NA hsa-miR-107 CHEK1 -0.121070851008387 0.198381281894242 0.573955749984589 8.28992689476689e-16 miRanda;miRNATAP -0.114487588080244 0.00132462804560562 NA NA NA hsa-miR-129-5p CHEK1 -0.567133990117126 0.00116152215679936 0.573955749984589 8.28992689476689e-16 miRanda -0.122309084327909 1.74565500015445e-11 NA NA NA hsa-miR-139-5p CHEK1 -0.647754756897633 2.15378307237163e-06 0.573955749984589 8.28992689476689e-16 miRanda -0.187266116631356 7.28244952419277e-16 NA NA NA hsa-miR-326 CHEK1 -0.631050656251074 8.83643450601471e-05 0.573955749984589 8.28992689476689e-16 miRanda -0.127671135956966 2.62556784627874e-11 NA NA NA hsa-miR-330-5p CHEK1 -0.262932095995605 0.0465226565966658 0.573955749984589 8.28992689476689e-16 miRanda -0.118282510893023 6.77322870635937e-07 NA NA NA hsa-miR-346 CHEK1 -0.577786425963268 2.10883270131593e-09 0.573955749984589 8.28992689476689e-16 miRanda -0.261286859784501 1.56543047407142e-16 NA NA NA hsa-miR-491-3p CHEK1 -0.523374665885358 1.93479565404951e-05 0.573955749984589 8.28992689476689e-16 MirTarget -0.175954445428317 2.99887467961377e-12 NA NA NA hsa-miR-99b-5p CHEK1 0.0938366784257259 0.419406306408393 0.573955749984589 8.28992689476689e-16 miRNAWalker2_validate -0.112056772233989 0.00170409121443777 NA NA NA hsa-miR-3065-5p CYCS -0.262608140481507 0.0414570974148034 0.024719343035275 0.675835191929842 mirMAP -0.160124769897675 9.92708866612115e-27 NA NA NA hsa-miR-576-5p CYCS 0.106482276040047 0.194379152564166 0.024719343035275 0.675835191929842 mirMAP -0.117195910223943 2.29387377994863e-06 NA NA NA hsa-miR-93-3p CYCS 0.0778767956494004 0.272224943247293 0.024719343035275 0.675835191929842 miRNAWalker2_validate -0.105610171429319 0.000386651303177803 NA NA NA hsa-miR-504-5p FAS -0.510726779625117 6.12122269907368e-05 0.265125211236005 0.00536978445115343 miRNAWalker2_validate -0.123587493507518 0.000186057016947978 NA NA NA hsa-miR-129-5p GADD45A -0.567133990117126 0.00116152215679936 0.578892696032557 1.73109905217762e-08 miRanda -0.117655587237976 4.85565235622386e-06 NA NA NA hsa-miR-504-5p GADD45A -0.510726779625117 6.12122269907368e-05 0.578892696032557 1.73109905217762e-08 miRNAWalker2_validate -0.168708037304693 1.35009592733874e-06 NA NA NA hsa-miR-767-3p GADD45B -0.429854368420513 0.000821445291167688 0.336709257563835 0.00421134915835084 miRNATAP -0.202993831911469 2.25161118087796e-07 NA NA NA hsa-miR-107 GADD45G -0.121070851008387 0.198381281894242 -0.285061728022188 0.0219160171580943 miRanda -0.395129731583354 1.47199359369081e-11 NA NA NA hsa-miR-127-3p GADD45G -0.164565333886312 0.2710118155902 -0.285061728022188 0.0219160171580943 miRanda;miRNATAP -0.22122061367908 1.46503376303966e-09 NA NA NA hsa-miR-1468-5p GADD45G -0.00455857880561572 0.960319769044627 -0.285061728022188 0.0219160171580943 MirTarget -0.287534063688522 5.82428979745539e-07 NA NA NA hsa-miR-1976 GADD45G 0.127074435034705 0.277235601775457 -0.285061728022188 0.0219160171580943 MirTarget -0.288291002566395 7.11683611697151e-11 NA NA NA hsa-miR-139-5p GTSE1 -0.647754756897633 2.15378307237163e-06 1.39568625070209 3.75113613545606e-22 miRanda -0.35004661461097 6.33473575341772e-13 NA NA NA hsa-miR-181c-5p GTSE1 -0.285631740307867 0.00281600994521758 1.39568625070209 3.75113613545606e-22 MirTarget -0.34959907191584 4.82570422233003e-06 NA NA NA hsa-miR-338-3p GTSE1 -0.39963704679384 0.0034410643220691 1.39568625070209 3.75113613545606e-22 miRanda -0.297095890669549 1.41590043202577e-08 NA NA NA hsa-miR-342-3p GTSE1 -0.125683829770877 0.105810751640137 1.39568625070209 3.75113613545606e-22 miRanda -0.246853922814393 0.00968624335014599 NA NA NA hsa-miR-346 GTSE1 -0.577786425963268 2.10883270131593e-09 1.39568625070209 3.75113613545606e-22 miRanda -0.532291433544917 7.74368310288451e-16 NA NA NA hsa-miR-490-3p GTSE1 -0.834268051506884 5.26203010743215e-06 1.39568625070209 3.75113613545606e-22 miRanda -0.22416672891566 3.4804548185714e-10 NA NA NA hsa-miR-491-5p GTSE1 -0.749881113074983 8.22859263179194e-09 1.39568625070209 3.75113613545606e-22 miRanda -0.396328091689019 2.33924258698768e-16 NA NA NA hsa-miR-543 GTSE1 -0.347737549779625 0.0157394585079683 1.39568625070209 3.75113613545606e-22 MirTarget;miRanda -0.150829340433235 0.00125135215758065 NA NA NA hsa-miR-766-3p GTSE1 -0.147747825439875 0.266658981351507 1.39568625070209 3.75113613545606e-22 MirTarget -0.202056315564114 3.67832207662707e-05 NA NA NA hsa-miR-320a IGF1 0.092135599636725 0.243028758989929 -0.203195580339987 0.029670632911231 miRNATAP -0.272065311188534 2.24035754570114e-05 NA NA NA hsa-miR-592 IGF1 0.0170005651576508 0.876633091134809 -0.203195580339987 0.029670632911231 mirMAP -0.113833175952146 0.00219422982453576 NA NA NA hsa-miR-9-3p IGF1 -0.184945845984695 0.0290051908009009 -0.203195580339987 0.029670632911231 MirTarget -0.387265166384702 1.83580721842057e-09 NA NA NA hsa-miR-3065-5p IGFBP3 -0.262608140481507 0.0414570974148034 0.813873247049911 5.13040813514096e-07 MirTarget -0.197111855208535 0.000317701746457494 NA NA NA hsa-miR-374b-5p IGFBP3 -0.210113949579608 0.0056866083818614 0.813873247049911 5.13040813514096e-07 MirTarget -0.298786997614656 0.00453049747951356 NA NA NA hsa-miR-9-5p IGFBP3 -0.125377971246422 0.269896477802284 0.813873247049911 5.13040813514096e-07 MirTarget;miRNATAP -0.835295489754643 4.84694667135587e-08 NA NA NA hsa-miR-3065-5p MDM2 -0.262608140481507 0.0414570974148034 0.179468554373188 0.00090619936510014 mirMAP -0.108004752778931 9.0880493197318e-14 NA NA NA hsa-miR-374b-5p MDM2 -0.210113949579608 0.0056866083818614 0.179468554373188 0.00090619936510014 mirMAP -0.115805376151209 4.29838757263469e-05 NA NA NA hsa-miR-504-5p MDM2 -0.510726779625117 6.12122269907368e-05 0.179468554373188 0.00090619936510014 miRNAWalker2_validate -0.122605313729819 6.11646737446648e-17 NA NA NA hsa-miR-7-1-3p MDM2 -0.0894475316162393 0.37322359785108 0.179468554373188 0.00090619936510014 mirMAP -0.103468424775092 4.37531630762283e-08 NA NA NA hsa-miR-9-3p MDM2 -0.184945845984695 0.0290051908009009 0.179468554373188 0.00090619936510014 mirMAP -0.122381796737815 5.87019491428867e-05 NA NA NA hsa-let-7b-5p MDM4 0.036218385218298 0.719557004564478 0.0648102987569947 0.345708692826051 miRNAWalker2_validate;MirTarget -0.109183389588662 0.00495397248931305 NA NA NA hsa-miR-107 MDM4 -0.121070851008387 0.198381281894242 0.0648102987569947 0.345708692826051 miRanda -0.149637201433794 1.12356165334077e-06 NA NA NA hsa-miR-125b-2-3p MDM4 -0.0775572585326127 0.381771874712035 0.0648102987569947 0.345708692826051 mirMAP -0.110261427965281 0.00110388700620945 NA NA NA hsa-miR-126-5p MDM4 0.177332403948664 0.0711675116243123 0.0648102987569947 0.345708692826051 MirTarget -0.135532106746436 3.2583603883033e-06 NA NA NA hsa-miR-140-3p MDM4 -0.123864258867705 0.14796686619449 0.0648102987569947 0.345708692826051 MirTarget -0.109780595937769 0.00990117645024203 NA NA NA hsa-miR-148b-3p MDM4 0.0710353380371513 0.322794068460232 0.0648102987569947 0.345708692826051 MirTarget -0.133852450917256 0.00243388769711256 NA NA NA hsa-miR-150-5p MDM4 0.0645271721519611 0.513295658828718 0.0648102987569947 0.345708692826051 MirTarget -0.13544278054786 2.45971123103794e-06 NA NA NA hsa-miR-192-5p MDM4 -0.0426540615456616 0.621616927627554 0.0648102987569947 0.345708692826051 miRNAWalker2_validate -0.129280429802387 0.00047833897218192 NA NA NA hsa-miR-29a-5p MDM4 -0.0697727833812678 0.350621682973119 0.0648102987569947 0.345708692826051 mirMAP -0.10210005799411 0.00660519007244013 NA NA NA hsa-miR-30d-3p MDM4 0.0854049347792898 0.20691254801121 0.0648102987569947 0.345708692826051 mirMAP -0.159207027364255 9.86420602511879e-05 NA NA NA hsa-miR-30d-5p MDM4 -0.0607010868720046 0.471410043548794 0.0648102987569947 0.345708692826051 mirMAP;miRNATAP -0.283270397963204 6.99646153515509e-09 NA NA NA hsa-miR-30e-5p MDM4 0.0764521828640934 0.41904991629622 0.0648102987569947 0.345708692826051 mirMAP -0.145357159965698 0.000378502521449909 NA NA NA hsa-miR-326 MDM4 -0.631050656251074 8.83643450601471e-05 0.0648102987569947 0.345708692826051 miRanda -0.100113657060335 1.94244801764792e-09 NA NA NA hsa-miR-3607-3p MDM4 -0.0118040987013632 0.907228689257085 0.0648102987569947 0.345708692826051 mirMAP -0.19658590549326 2.3612062892002e-13 NA NA NA hsa-miR-488-3p MDM4 -0.257955761012568 0.00280795162806256 0.0648102987569947 0.345708692826051 mirMAP -0.166044242655751 2.64307325949592e-07 NA NA NA hsa-miR-491-5p MDM4 -0.749881113074983 8.22859263179194e-09 0.0648102987569947 0.345708692826051 miRanda -0.115644309340274 1.5734793639656e-08 NA NA NA hsa-miR-7-1-3p MDM4 -0.0894475316162393 0.37322359785108 0.0648102987569947 0.345708692826051 mirMAP -0.104734705391964 0.00013937072910631 NA NA NA hsa-miR-766-3p MDM4 -0.147747825439875 0.266658981351507 0.0648102987569947 0.345708692826051 MirTarget -0.108960060599752 7.69310725236255e-08 NA NA NA hsa-miR-98-5p MDM4 -0.0806971683871618 0.251777941211683 0.0648102987569947 0.345708692826051 MirTarget -0.138052928606747 0.00183397997033713 NA NA NA hsa-miR-125a-5p PPM1D -0.0833349424517387 0.325440200253607 0.223880834234628 4.58568238564558e-06 miRanda -0.218767262976163 2.40761181086109e-16 NA NA NA hsa-miR-26b-5p PPM1D -0.0984536538762768 0.231803769574623 0.223880834234628 4.58568238564558e-06 miRNAWalker2_validate -0.14060431062657 2.29225976378235e-07 NA NA NA hsa-miR-29a-3p PPM1D -0.224140851576083 0.0349516103550236 0.223880834234628 4.58568238564558e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.164513639603831 3.97701972866745e-13 NA NA NA hsa-miR-29a-5p PPM1D -0.0697727833812678 0.350621682973119 0.223880834234628 4.58568238564558e-06 MirTarget -0.129752810702726 3.74797795198897e-07 NA NA NA hsa-miR-29b-3p PPM1D -0.178522712044497 0.0856106432165465 0.223880834234628 4.58568238564558e-06 MirTarget;miRNATAP -0.175389086111835 4.34809545023955e-19 NA NA NA hsa-miR-29c-3p PPM1D -0.372180390387259 0.00202634431973208 0.223880834234628 4.58568238564558e-06 miRNAWalker2_validate;MirTarget;miRNATAP -0.142127421299469 1.42992603116014e-17 25888625 A suppressive role of ionizing radiation responsive miR 29c in the development of liver carcinoma via targeting WIP1; miR-29c expression is downregulated in human hepatocellular carcinoma cells which is inversely correlated with WIP1 expression; miR-29c attenuates luciferase activity of a reporter harboring the 3'UTR binding motif of WIP1 mRNA; The biological effects of miR-29c may be mediated by its target WIP1 which regulates p53 activity via dephosphorylation at Ser-15; Finally fluorescence in situ hybridization FISH and immunohistochemical analyses indicate that miR-29c is downregulated in 50.6% of liver carcinoma tissues examined whereas WIP1 is upregulated in 45.4% of these tissues; The expression of miR-29c inversely correlates with that of WIP1 in HCC; Our results suggest that the IR-responsive miR-29c may function as a tumor suppressor that plays a crucial role in the development of liver carcinoma via targeting WIP1 therefore possibly representing a target molecule for therapeutic intervention for this disease liver cancer hsa-miR-346 PPM1D -0.577786425963268 2.10883270131593e-09 0.223880834234628 4.58568238564558e-06 miRanda -0.114861179220204 1.83877178908115e-09 NA NA NA hsa-miR-361-5p PPM1D 0.0107519600643302 0.882230614095565 0.223880834234628 4.58568238564558e-06 miRanda;miRNATAP -0.192215614666882 1.55839584466538e-09 NA NA NA hsa-miR-491-5p PPM1D -0.749881113074983 8.22859263179194e-09 0.223880834234628 4.58568238564558e-06 miRanda -0.111452384401258 6.69264265195707e-16 NA NA NA hsa-miR-25-3p PTEN 0.316649850160985 0.000777982468387578 -0.166774236847838 0.000693239621129742 miRTarBase;MirTarget;miRNATAP -0.10796918628155 5.86307501062941e-07 NA NA NA hsa-miR-320a PTEN 0.092135599636725 0.243028758989929 -0.166774236847838 0.000693239621129742 MirTarget;PITA;miRanda;miRNATAP -0.120673517851669 1.04464750662028e-06 NA NA NA hsa-miR-320b PTEN 0.283477746304177 0.0005953763020907 -0.166774236847838 0.000693239621129742 MirTarget;PITA;miRanda;miRNATAP -0.101263900507425 6.87434169702041e-08 NA NA NA hsa-miR-93-5p PTEN 0.139766237971122 0.101057320076629 -0.166774236847838 0.000693239621129742 miRNAWalker2_validate;miRTarBase;miRNATAP -0.127367364164339 6.57333465080741e-06 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-542-3p RCHY1 0.0835995677900829 0.403838744494071 -0.192868555091836 1.63704213384007e-06 miRNATAP -0.109126940296307 1.31047035528688e-14 NA NA NA hsa-miR-335-5p RPRM 0.0938511632756978 0.336889056387694 -0.831926532364272 1.11175951229609e-09 MirTarget;miRNATAP -0.232392053463486 0.000194426161261015 NA NA NA hsa-miR-423-5p RPRM 0.0965587900813789 0.148039180704338 -0.831926532364272 1.11175951229609e-09 MirTarget -0.455316585063038 6.05387285555132e-06 NA NA NA hsa-miR-501-3p RPRM 0.0726267165568464 0.382729320534971 -0.831926532364272 1.11175951229609e-09 miRNATAP -0.475849890769315 1.16595506155945e-10 NA NA NA hsa-let-7e-5p RRM2 0.0471189209570344 0.657255455444655 1.72962461314845 1.1581260579143e-19 miRNATAP -0.279692903103837 0.00364962053307039 NA NA NA hsa-let-7g-5p RRM2 -0.0253396334608382 0.742556489443668 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate;miRNATAP -0.495185466781977 0.000760406582174647 NA NA NA hsa-miR-100-5p RRM2 0.0939087252225708 0.437623927320764 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate -0.244948738958063 0.00506316871324009 NA NA NA hsa-miR-125a-5p RRM2 -0.0833349424517387 0.325440200253607 1.72962461314845 1.1581260579143e-19 miRanda -0.722263621277643 7.13952812151879e-09 NA NA NA hsa-miR-26a-5p RRM2 0.033823776747294 0.726610414342369 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate -0.303789574350027 0.00524886414864605 NA NA NA hsa-miR-30a-5p RRM2 -0.062942936611238 0.513851954700252 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate -0.774981141398335 1.45719574576206e-07 NA NA NA hsa-miR-30c-5p RRM2 -0.0469114469943097 0.565934557464627 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate -0.611695991452107 7.10703705683768e-06 NA NA NA hsa-miR-342-3p RRM2 -0.125683829770877 0.105810751640137 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate;miRanda -0.356992143743657 0.00438084834317225 NA NA NA hsa-miR-421 RRM2 -0.171573235029879 0.0518112933651029 1.72962461314845 1.1581260579143e-19 miRanda -0.317127561186805 0.00130125216463725 NA NA NA hsa-miR-484 RRM2 -0.0190828819137732 0.802298900989652 1.72962461314845 1.1581260579143e-19 miRNAWalker2_validate -0.370574235214709 0.00242368401630534 NA NA NA hsa-miR-186-5p RRM2B 0.0606381894130505 0.514217717592464 0.038399701626723 0.474347124721092 mirMAP -0.163443533438203 8.55974174989147e-13 NA NA NA hsa-miR-30c-5p RRM2B -0.0469114469943097 0.565934557464627 0.038399701626723 0.474347124721092 mirMAP -0.153012706644655 6.7784613295446e-07 NA NA NA hsa-miR-124-3p SERPINE1 -0.506026514549291 0.0078056370358648 1.27620546981704 1.91626732714512e-12 miRNAWalker2_validate -0.11053232774917 0.00933875755402922 NA NA NA hsa-miR-129-5p SERPINE1 -0.567133990117126 0.00116152215679936 1.27620546981704 1.91626732714512e-12 miRanda -0.136388531449073 0.00376304958296275 NA NA NA hsa-miR-138-5p SERPINE1 -0.616087649920851 1.35200027885729e-05 1.27620546981704 1.91626732714512e-12 miRNAWalker2_validate -0.27183048471367 1.58790910020002e-06 NA NA NA hsa-miR-767-3p SERPINE1 -0.429854368420513 0.000821445291167688 1.27620546981704 1.91626732714512e-12 PITA -0.360745702058261 1.27615948946032e-08 NA NA NA hsa-miR-15b-5p SESN1 0.592863156692837 1.41971517354055e-10 -0.369459276277261 1.3742368209488e-07 MirTarget;miRNATAP -0.191658244672968 1.65302880062613e-09 NA NA NA hsa-miR-200c-3p SESN1 0.413752622631695 8.6936333715621e-06 -0.369459276277261 1.3742368209488e-07 MirTarget;miRNATAP -0.12529105266438 3.47381629017008e-05 24791940 We found that miR-200c overexpression increased cellular radiosensitivity by direct regulation of the oxidative stress response genes PRDX2 GAPB/Nrf2 and SESN1 in ways that inhibits DNA double-strand breaks repair increase levels of reactive oxygen species and upregulate p21 drug resistance lung cancer hsa-miR-424-5p SESN1 0.25140374049676 0.00709934939774983 -0.369459276277261 1.3742368209488e-07 MirTarget;miRNATAP -0.163817950984226 1.99961550672545e-07 NA NA NA hsa-miR-23b-3p SESN2 0.0476610245860094 0.630277683372344 0.202952431661197 0.00028888392689232 miRNATAP -0.124962029205828 2.1007966810668e-07 NA NA NA hsa-miR-27b-3p SESN2 -0.0599187745243146 0.58208143475992 0.202952431661197 0.00028888392689232 miRNATAP -0.113811566870458 1.36776420626997e-07 NA NA NA hsa-miR-146b-5p SESN3 0.441078059667297 0.000237619929889385 -0.0656016620402924 0.552417247655311 PITA;miRanda;miRNATAP -0.120927107890466 0.00342788133630851 NA NA NA hsa-miR-20a-5p SESN3 -0.0115865752288853 0.887069448776018 -0.0656016620402924 0.552417247655311 MirTarget -0.321175445764073 3.74683904084502e-06 NA NA NA hsa-miR-320a SESN3 0.092135599636725 0.243028758989929 -0.0656016620402924 0.552417247655311 miRanda;mirMAP -0.292586084185307 9.45135212669266e-05 NA NA NA hsa-miR-454-3p SESN3 0.177318549827649 0.0120865170334418 -0.0656016620402924 0.552417247655311 mirMAP -0.191985281390642 0.00615053083247489 NA NA NA hsa-miR-500a-5p SESN3 0.0634452729978863 0.396072382601677 -0.0656016620402924 0.552417247655311 mirMAP -0.246256084148311 0.000107167051359629 NA NA NA hsa-miR-9-3p SESN3 -0.184945845984695 0.0290051908009009 -0.0656016620402924 0.552417247655311 MirTarget -0.401701336702146 9.96760616230229e-08 NA NA NA hsa-miR-1296-5p SHISA5 -0.54372484392435 2.8372994306455e-07 0.253265339539354 1.31377626877513e-05 miRNAWalker2_validate -0.118036778805522 5.123336766207e-10 NA NA NA hsa-miR-193b-3p SIAH1 0.0766333116224072 0.324723824453143 0.00581809385550081 0.890923204236709 miRNATAP -0.135133349463128 3.54935240682215e-14 NA NA NA hsa-miR-200b-3p SIAH1 0.245768526504511 0.0580744465744737 0.00581809385550081 0.890923204236709 TargetScan -0.116917198908622 9.27165175527678e-32 NA NA NA hsa-miR-30a-3p SIAH1 0.0655410309550977 0.509541256241954 0.00581809385550081 0.890923204236709 miRNATAP -0.201715217879709 4.71664770108326e-34 NA NA NA hsa-miR-421 SIAH1 -0.171573235029879 0.0518112933651029 0.00581809385550081 0.890923204236709 miRanda -0.11373692685153 3.28392408036691e-14 NA NA NA hsa-miR-488-5p SIAH1 -0.385755602369049 7.99431673275965e-08 0.00581809385550081 0.890923204236709 miRNATAP -0.102522963028622 1.14924955661141e-07 NA NA NA hsa-miR-532-5p SIAH1 0.0263466708637097 0.75763006039876 0.00581809385550081 0.890923204236709 PITA;miRNATAP -0.127320129384503 1.86784904104102e-11 NA NA NA hsa-miR-128-3p STEAP3 -0.781932174412672 7.1812756321342e-10 0.851505451100282 5.28142587331167e-10 MirTarget -0.37490843499386 2.7467323425801e-13 NA NA NA hsa-miR-490-3p STEAP3 -0.834268051506884 5.26203010743215e-06 0.851505451100282 5.28142587331167e-10 miRanda -0.137561425956177 4.05745773296894e-05 NA NA NA hsa-miR-9-5p STEAP3 -0.125377971246422 0.269896477802284 0.851505451100282 5.28142587331167e-10 MirTarget -1.07056472415579 2.71982697310106e-16 NA NA NA hsa-miR-338-3p THBS1 -0.39963704679384 0.0034410643220691 0.542687032308904 0.000869835800177595 MirTarget;PITA;miRanda -0.218145983536744 0.000122986602033708 NA NA NA hsa-miR-26b-5p TNFRSF10B -0.0984536538762768 0.231803769574623 0.334610949770233 2.80844341840702e-05 mirMAP -0.145112479707621 0.00300053851841411 NA NA NA hsa-miR-3065-5p TNFRSF10B -0.262608140481507 0.0414570974148034 0.334610949770233 2.80844341840702e-05 mirMAP -0.219007549297091 5.79943007315713e-18 NA NA NA hsa-miR-30c-1-3p TNFRSF10B 0.0175008295781193 0.849381871022214 0.334610949770233 2.80844341840702e-05 MirTarget -0.162621201182926 1.0567643617516e-05 NA NA NA hsa-miR-331-3p TNFRSF10B 0.0126984726684189 0.905175053956974 0.334610949770233 2.80844341840702e-05 miRNAWalker2_validate -0.139320916932824 1.1678251691796e-05 NA NA NA hsa-miR-3622a-3p TNFRSF10B 0.0389203848593471 0.75605812962459 0.334610949770233 2.80844341840702e-05 mirMAP -0.109923831583013 5.54121970241808e-05 NA NA NA hsa-miR-940 TNFRSF10B -0.0183807673806575 0.881747775878136 0.334610949770233 2.80844341840702e-05 miRNAWalker2_validate -0.152290945518939 1.36170749375405e-08 NA NA NA hsa-miR-125a-5p TP53 -0.0833349424517387 0.325440200253607 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase;miRanda -0.375445863722298 1.2184854773952e-16 26389681;21777146;23079745 Mechanistically inactivation of miR-125a during cervical carcinogenesis was caused by HPV suppression of p53 expression;In addition wild-type p53 mRNA and protein expression was increased by hsa-miR-125a-5p overexpression; Moreover blocking wild-type p53 attenuated the effect of hsa-miR-125a-5p on apoptosis; In loss-of-function experiments wild-type p53 mRNA and protein expression was decreased by blocking hsa-miR-125a-5p; The effect of hsa-miR-125a-5p inhibitor on apoptosis was also weakened by blocking wild-type p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity tumorigenesis;; cervical and endocervical cancer;lung cancer;liver cancer hsa-miR-125b-5p TP53 -0.0119698382910496 0.890233923197358 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase -0.294628443669371 1.07324994036762e-07 24169356;24846940;26335100;24137477;26686386;24402874;21399871;23585871;24762088;23079745 MiR 125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK independent pathways; Further studies reveal that p53 is regulated by miR-125b; However downregulation of the endogenous miR-125b also results in p53-independent apoptotic pathway leading to apoptosis in p53 mutated U251 cells and p53 knockdown U87 cells;The results revealed that hsa-miR-125b may regulate multiple biological processes and signal transduction pathways and drug-resistant occurrence is associated with cell proliferation cell apoptosis cell cycle and signaling pathways including MAPK Wnt and p53;The carboxy terminal domain of connexin 43 CT Cx43 modulates the expression of p53 by altering miR 125b expression in low grade human breast cancers; In addition CT-Cx43 was exogenously expressed in the breast cancer-derived cell line MCF-7 and its effect on the expression of miR-125b and its downstream target p53 were evaluated as well as its effect on cellular proliferation and death using MTT and LDH assays respectively; Interestingly we found that miR-125b a negative regulator of p53 exhibited an inverse expression relationship with CT-Cx43 in the breast cancer samples tested;TP53 and hsa-miR-125b were observed to form a self-adaptation association;In this study we have investigated the delivery and transfection of wild-type wt- p53 and microRNA-125b miR-125b expressing plasmid DNA in SK-LU-1 human lung adenocarcinoma cells as well as in KrasG12D/p53fl/fl KP genetically engineered mouse model of lung cancer;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;In vitro assays revealed that overexpression of miR-125b repressed the endogenous level of p53 protein in human colorectal cancer cells;In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b;A stable overexpression of miR-125b in human melanoma cell line Mel-Juso resulted in a G0/G1 cell cycle block and emergence of large cells expressing senescence markers: senescence-associated beta-galactosidase p21 p27 and p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity; To show the clinical significance of these findings mutations in the DNA binding domain of p53 and promoter methylation of miR-125b were investigated; Four out of nine patients with induced SIRT7 carried mutations in the p53 gene and one patient showed hypermethylation of the miR-125b promoter region ;;;;;immune resistance;;;; glioblastoma;gastric cancer;breast cancer;pancreatic cancer;lung cancer;cervical and endocervical cancer;colorectal cancer;prostate cancer;melanoma;liver cancer hsa-miR-150-5p TP53 0.0645271721519611 0.513295658828718 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.156281214913021 3.09404029899862e-06 23747308;23228962 miR 150 promotes the proliferation of lung cancer cells by targeting P53; Here we demonstrate that miR-150 is aberrantly upregulated in lung cancer tissue and negatively correlates with the expression of the proapoptotic gene p53 but not EGR2; We show that miR-150 specifically targets the 3'-UTR of p53 and regulates its expression; Inhibition of miR-150 effectively delays cell proliferation and promotes apoptosis accompanied by increased p53 protein expression;miR-150 and miR-3940-5p were found to be significantly downregulated in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA; miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect pathway ; lung cancer;lung squamous cell cancer hsa-miR-221-3p TP53 -0.0109507285071775 0.936440693394519 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.143405645468124 6.89189438710031e-10 20880178;24324033 Circulating miR 221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression; The correlation between miR-221 levels and protein levels of p53 CEA ER and PR clinicopathological features or overall survival was analyzed; The immunohistochemistry analysis demonstrates a significant correlation between plasma miR-221 level and p53 expression; The direct amplification of plasma miR-221 can be used as a potential noninvasive molecular marker for diagnosis and prognosis of CRC and is correlated with p53 expression;Interestingly miR-221 can activate the p53/mdm2 axis by inhibiting MDM2 and in turn p53 activation contributes to miR-221 enhanced expression; Moreover by modulating the p53 axis miR-221 impacts cell-cycle progression and apoptotic response to doxorubicin in hepatocellular carcinoma-derived cell lines; These data were confirmed in clinical specimens of hepatocellular carcinoma in which elevated miR-221 expression was associated with the simultaneous presence of wild-type p53 and DNA hypomethylation poor survival;worse prognosis;progression;drug resistance colorectal cancer;liver cancer hsa-miR-222-3p TP53 0.107117065575197 0.423818119725697 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.142653482163308 1.2671214916531e-09 NA NA NA hsa-miR-30d-5p TP53 -0.0607010868720046 0.471410043548794 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.336903694979927 3.32137008004591e-09 NA NA NA hsa-miR-324-5p TP53 0.0183370482495953 0.842996782677271 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.170645382602635 1.21583558752455e-06 NA NA NA hsa-miR-338-3p TP53 -0.39963704679384 0.0034410643220691 0.346312114104146 1.42081328137217e-05 miRanda -0.117560999445537 4.2049029410775e-06 NA NA NA hsa-miR-34a-5p TP53 0.143472919835524 0.211283228567057 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.127236433475415 7.74576992902642e-06 23155233;19773441;25572695;25123132;22438124;21702042;22292433;21383543;25982144;19443717;26790955;24642471;22198213;25362853;23036084;19421141;24444609;20186752;22457788;27186405;21731696;19736307;26879132;23569431;25771001;23292869;24209638;24957404;21240262;24503183;22102859;18803879;24630988;25895459;25894979;19714243;26944831;19921694;21909380;25789847;23450486;24402874;24337371;18497571;25490093;22593438;24528540;20145172;23349340;25038915;21321636;23632240;23624843;19029026;20309940;22810507;23862748;25932212;18834855;21225432 The high miR-34a expression level in the cells after irradiation at 60 Gy reduced the p53 expression level;MicroRNA-34a miR-34a is a transcriptional target of p53 that is down-regulated in some cancer cell lines; Analysis of human specimens showed that miR-34a expression is down-regulated in glioblastoma tissues as compared with normal brain and in mutant p53 gliomas as compared with wild-type p53 gliomas;For this purpose cell viability assay Realtime quantitative PCR for mRNA quantification western blot for essential protein expression p53 silencing by shRNA and miR-34a knockdown were performed in the present study; PRE also elevated p53 protein and triggered miR-34a expression;As expected p53 loss caused downregulation of established p53 targets e.g p21 and miR-34 family and increased proliferation in both luminal and basal-like cell lines;Moreover re-expression of miR-34a by transfection in NSCLC cells resulted in inhibition of cell growth and invasiveness induction of apoptosis and enhanced p53 activity;MiR 34a chemosensitizes bladder cancer cells to cisplatin treatment regardless of p53 Rb pathway status; MiR-34a is a downstream effector of p53 that has been shown to target several molecules associated with cell cycle and cell survival pathways; As alterations in these pathways are frequent in muscle invasive transitional cell carcinoma of the bladder MI-TCC for example mutation or loss of p53 and Rb the goal of this study was to determine whether manipulation of miR-34a expression levels could abrogate the effect of these alterations and sensitize bladder cancer cells to chemotherapy;Reporter assay further showed that NF-kappaB-induced miR-34a transcriptional activity was reduced by p53 impairment; And wildtype p53 is responsible for NF-kappaB-mediated miR-34a transcriptional activity but not for NF-kappaB binding;Recently miR-34 family has been shown to be part of the p53 pathway which is frequently involved in lung cancer and the expression of miR-34 has been reported to be regulated by DNA methylation;5 Aminolevulinic acid mediated sonodynamic therapy induces anti tumor effects in malignant melanoma via p53 miR 34a Sirt1 axis; Therefore the p53 miR-34a and SIRT1 constituted a positive feedback loop;miR-34a expression could be increased in Y79 cells but not Weri-Rb1 cells after p53 activation; This differential regulation was not caused by genomic alterations at the miR-34a p53 binding site or mature gene;Conversely kallistatin stimulated expression of the tumorigenic suppressors miR-34a and p53;Members of the miR-34 family are induced by the tumor suppressor p53 and are known to inhibit epithelial-to-mesenchymal transition EMT and therefore presumably suppress the early phases of metastasis;MicroRNA-34a miR-34a a transcriptional target of p53 is a well-known tumor suppressor gene; Moreover we found that miR-34a was downregulated in 25 of 40 62.5% colon cancer tissues as compared with the adjacent normal colon tissues and that the expression of miR-34a was correlated with the DNA-binding activity of p53;Ectopic expression of miR-34a-5p in p53 wild-type colon cancer cell HCT116 significantly inhibited cell growth migration invasion and metastasis; miR-34a-5p induced cell apoptosis cell cycle arrest at G1 phase and p53 transcription activity in HCT116 cells but not in the HCT116 p53 knockout p53-/- cells; miR-34a-5p significantly suppressed the HCT116 growth in vivo whereas it showed no effect on the HCT116 p53-/- xenograft indicating that the growth-inhibiting effect by miR-34a-5p was dependent on p53;microRNA 34a sensitizes lung cancer cell lines to DDP treatment independent of p53 status; However the precise biological role of miR-34a in p53 deficient lung cancer cell lines remains largely elusive; Overall in this study we found the proliferation inhibition function of miR-34a in vitro in lung cancer cell lines is p53 independent and also demonstrated the combination therapeutic potential of miR-34a and DDP in lung cancer cell lines;In particular mammalian miR-34 is upregulated by p53 in response to radiation but little is known about the role of this miRNA in vivo; These findings show a role for mir-34 in both apoptotic and non-apoptotic cell death in vivo much like that of cep-1 the C elegans p53 homolog;Tumor-suppressive miR-34a a direct target of p53 has been shown to target several molecules of cell survival pathways; Here we show that capsaicin-induced oxidative DNA damage culminates in p53 activation to up-regulate expression of miR-34a in non-small cell lung carcinoma NSCLC cells;Accumulating evidence suggests that miR-34a as a key mediator of p53 tumor suppression is aberrantly expressed in human cancers; Bioinformatics analysis produced a protein-protein interaction network which revealed that the p53 signaling pathway and cell cycle pathway were two major hubs containing most of the proteins regulated by miR-34a;Studies have demonstrated that miR-34a which is a direct target of the p53 tumor suppressor gene functions as a tumor suppressor and is associated with the tumor growth and metastasis of various human malignances;Knockdown of oncoprotein E6 expression of human papillomavirus in SiHa and HeLa cells by siRNAs lead to an increased protein level of p53 decreased level of miR-34a as well as reduced Warburg effect;miR-34a is known as a p53 regulated tumor suppressor microRNA in many cancer types;In addition in patients with sufficient tumor tissue we assessed p53 mutations and the methylation status of the MIRN34A gene promoter region and correlated these findings with miR-34a expression; Patients with both p53 mutations and low miR-34a levels had the highest probability of relapse P = 0.001;Particularly miR-34a has been revealed to be a direct transcriptional target of p53 which is frequently mutated in epithelial ovarian carcinomas especially in high grade serous cancer; The aim of this study was to investigate the clinical relevance of mir34a as well as its promoter methylation in a subset of 133 ovarian cancers with a special focus on the p53 mutation status the dualistic type I and type II ovarian cancer model and the different histotypes; The expression of miR-34a was found lower in type II than in type I cancers p = 0.037 in p53 mutated as compared to p53 wild type cancers p = 0.003 and in high grade compared to in low grade cancers p = 0.028; The inverse association between miR-34a expression and grading p53 mutation status and dualistic tumor type classification together with its prognostic relevance may underline the tumor-suppressive character of miR-34a in ovarian cancer;As a direct target gene of p53 miR-34a has been suggested to mediate the tumor suppressor function of p53;Further studies indicated that PEG4000-TQ-Nps could significantly increase the expression of miR-34a through p53;Downregulation of miR 34a in breast tumors is not associated with either p53 mutations or promoter hypermethylation while it correlates with metastasis; Recent studies have shown that p53 upregulates miR-34 family leading to direct repression of several key oncogenes; In this study expression of mature miR-34a in breast tumors with wild-type p53 was investigated in order to find any correlation between dysregulation of miR-34a expression and breast cancer; In about 40 % of the wild-type p53 samples miR-34a was significantly downregulated; This study has provided evidence that miR-34a expression can be affected in a significant proportion of breast tumors independent of p53; Knowledge of miR-34a status may provide additional useful information regarding the nature of breast tumors especially when p53 testing does not show any aberration;Among these we found well studied molecules such as the miR-17-92 cluster comprising potent oncogenic microRNA and miR-34 recently found to interact with p53;The results demonstrated that miR-199a and miR-34a could induce the apoptosis of human osteosarcoma cells via p53 signalling pathway;Here we show through expression analysis that miR-34a a p53 target was underexpressed in CD44+ prostate cancer cells purified from xenograft and primary tumors;Members of the miR-34 family have been shown to be transcriptional targets of the tumour suppressor gene P53;Functional genomic analysis highlighted a novel regulatory role of the transcription factor MAZ apart from the known control by p53 on the expression of miR-34a and a number of miR-34a targets; Our analysis for regulatory loops suggest that MAZ and p53 transcription factors co-operate in modulating miR-34a as well as miR-34a targets involved in several cellular pathways;Restoration of tumor suppressor miR 34 inhibits human p53 mutant gastric cancer tumorspheres; MicroRNA miR-34 was recently found to be a direct target of p53 functioning downstream of the p53 pathway as a tumor suppressor; Our results demonstrate that in p53-deficient human gastric cancer cells restoration of functional miR-34 inhibits cell growth and induces chemosensitization and apoptosis indicating that miR-34 may restore p53 function;miR 34 cooperates with p53 in suppression of prostate cancer by joint regulation of stem cell compartment; The miR-34 family was originally found to be a direct target of p53 and is a group of putative tumor suppressors; Here we report that mice with prostate epithelium-specific inactivation of mir-34 and p53 show expansion of the prostate stem cell compartment and develop early invasive adenocarcinomas and high-grade prostatic intraepithelial neoplasia whereas no such lesions are observed after inactivation of either the mir-34 or p53 genes alone by 15 months of age; Consistently combined deficiency of p53 and miR-34 leads to acceleration of MET-dependent growth self-renewal and motility of prostate stem/progenitor cells; Our study provides direct genetic evidence that mir-34 genes are bona fide tumor suppressors and identifies joint control of MET expression by p53 and miR-34 as a key component of prostate stem cell compartment regulation aberrations in which may lead to cancer;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however the effect of miR-34a on the ovarian cancer cells remains unclear;The microRNA-34 family miR-34a -34b and -34c have been reported to be tumor suppressor microRNAs miRNAs that are regulated by the TP53 and DNA hypermethylation; To elucidate the roles of miR-34 family in colon carcinogenesis miR-34a/b/c were measured in tumors and adjacent noncancerous tissues from 159 American and 113 Chinese colon cancer patients using quantitative RT-PCR and we examined associations between miR-34a/b/c expression with TNM staging cancer-specific mortality TP53 mutation status and Affymetrix microarray data;Transcription of the three miRNA miR-34 family members was recently found to be directly regulated by p53; Our results demonstrate that miR-34 may restore at least in part the tumor suppressing function of the p53 in p53-deficient human pancreatic cancer cells;p53 status was not correlated with miR-34a;The above results suggest that microRNA-34a is one of the important components of PRIMA-1-induced apoptotic network in the cancer cells harboring mutant p53;MicroRNA-34a miR-34a is a transcriptional target of p53 and is down-regulated in pancreatic cancer;Phytochemical regulation of the tumor suppressive microRNA miR 34a by p53 dependent and independent responses in human breast cancer cells; The tumor suppressive microRNA miR-34a is transcriptionally regulated by p53 and shown to inhibit breast cancer cell proliferation as well as being a marker of increased disease free survival; Human breast cancer cells expressing wild-type MCF-7 or mutant p53 T47D were treated with a concentration range and time course of each phytochemical under conditions of cell cycle arrest as detected by flow cytometry to examine the potential connection between miR-34a expression and their anti-proliferative responses; Our results suggest that miR-34a is an essential component of the anti-proliferative activities of I3C artemisinin and artesunate and demonstrate that both wild-type p53 dependent and independent pathways are responsible for miR-34a induction;Compared with p53 normal group the expression level of miR-34a was significantly lower in p53 deletion group;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;The microRNA miR-34 family is a direct transcriptional target of tumor-suppressor TP53 and loss of miR-34 function may impair TP53-mediated cell cycle arrest and apoptosis;It appears that AR-dependent inhibition of p53 resulted in suppression of miR-34a and -34c expression;In this study we evaluated the cascade of events determined by etoposide-induced DNA damage in OS cell lines with different p53 status focusing on methylation status and expression of miR-34a that modulate tumor cell growth and cell cycle progression; Our results suggest that the open and unmethylated conformation of the miR-34a gene may be regulated by p53 able to bind the gene promoter;The miR-34 family under-expressed in-non small cell lung cancers NSCLCs are effectors of p53 activation upon irradiation of cells;MiR-34a a direct p53 target gene possesses tumor-suppressive properties as they mediate apoptosis cell cycle arrest and senescence;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in human epithelial ovarian cancer EOC; miR-34a expression is decreased in 100% and miR-34b*/c in 72% of EOC with p53 mutation whereas miR-34a is also downregulated in 93% of tumors with wild-type p53; Finally miR-34 reconstitution experiments in p53 mutant EOC cells resulted in reduced proliferation motility and invasion the latter of which was dependent on MET expression;miR-34a is transcriptionally induced by the tumor suppressor gene p53 which is often downregulated in non-small cell lung cancer NSCLC;In conclusion overexpression of PEBP4 reduced the sensitivity of A549 cells to DDP-induced cytotoxicity mainly through the altered expression of the p53 protein or the modulation of miR-34a;To investigate the effects of miR-449 and miR-34 on cell growth cell cycle and target gene expression based on these miRNA different expressions in ovarian cancer cell lines SKOV3 and SKOV3-ipl both with mutation of p53;Numerous studies have focused on the association between miR-34 family members which are direct p53 targets and carcinogenesis of many cancers including hepatocellular carcinoma HCC;p53 regulates nuclear GSK 3 levels through miR 34 mediated Axin2 suppression in colorectal cancer cells;MicroRNA-34a miR-34a a potential key effector of the p53 tumor-suppressor gene was studied as a potential tumor suppressor in uveal melanoma;The miR-34 family members are direct transcriptional targets of tumor suppressor p53 and loss of miR-34 function can impair p53-mediated cell cycle arrest and apoptosis;Profiling microRNA miRNA expression delineated TP53 alteration-associated miRNA profiles and identified miR-34a and miR-100 as the most significantly down- and upregulated miRNA respectively; Clinically low miR-34a expression and TP53 alterations predicted for chemotherapy resistance and inferior outcome; Thus detailed molecular profiling links impaired p53 to decreased miR-34a expression but also identifies p53-independent miR-34a induction mechanisms as shown in TP53biallelic altered cell lines treated with 15-deoxy-Δ1214-prostaglandin;MiR-34a a direct target of p53 has been shown to target several molecules associated with the cell cycle and cell survival pathways and its dysregulation is implicated in cancer drug resistance or sensitivity in several human cancers;MicroRNA-34a miR-34a is a direct transcriptional target of p53 and links tumor suppressor function and the oncogenic pathways in some cancers;Tumor suppressor p53 transcriptionally regulates expression of microRNA-34a which confers translational inhibition and mRNA degradation of genes involved in cell cycle control and apoptosis; MiR-34a expression was markedly reduced in p53-null PC3 cells and p53-mutated DU145 cells compared with LNCaP cells expressing wild-type p53;The microRNA encoding genes miR-34a and miR-34b/c represent direct p53 target genes and possess tumor suppressive properties as they mediate apoptosis cell cycle arrest and senescence; In the colorectal cancer samples a statistically significant correlation of miR-34a methylation and the absence of p53 mutation was detected; The mutual exclusiveness of miR-34a methylation and p53 mutation indicates that miR-34a inactivation may substitute for loss of p53 function in cancer ;;;;;poor survival;;;malignant trasformation;;;metastasis;;metastasis;;drug resistance;poor survival;;metastasis;;;;;;;metastasis;;;;;;;motility;;staging;tumorigenesis;;;;;drug resistance;poor survival;;immune resistance;;;progression;;;motility;;;;tumorigenesis;;;;drug resistance;poor survival;drug resistance;;; glioblastoma;glioblastoma;bladder cancer;breast cancer;lung squamous cell cancer;bladder cancer;esophageal cancer;lung squamous cell cancer;melanoma;retinoblastoma;breast cancer;colorectal cancer;colon cancer;colorectal cancer;lung cancer;breast cancer;lung squamous cell cancer;liver cancer;sarcoma;cervical and endocervical cancer;lung squamous cell cancer;lung squamous cell cancer;ovarian cancer;sarcoma;breast cancer;breast cancer;head and neck cancer;sarcoma;prostate cancer;kidney renal cell cancer;breast cancer;gastric cancer;prostate cancer;ovarian cancer;colon cancer;pancreatic cancer;esophageal cancer;lung cancer;pancreatic cancer;breast cancer;B cell lymphoma;cervical and endocervical cancer;colorectal cancer;prostate cancer;sarcoma;lung squamous cell cancer;esophageal cancer;ovarian cancer;lung squamous cell cancer;lung cancer;ovarian cancer;liver cancer;colorectal cancer;melanoma;liver cancer;acute myeloid leukemia;liver cancer;gastric cancer;prostate cancer;sarcoma hsa-miR-380-5p TP53 -0.356387125389103 0.0163515117264043 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.130032370502874 2.53621800466177e-09 NA NA NA hsa-miR-381-3p TP53 -0.192118086401438 0.203198554750071 0.346312114104146 1.42081328137217e-05 MirTarget -0.10958470195975 2.36383687034549e-07 NA NA NA hsa-miR-421 TP53 -0.171573235029879 0.0518112933651029 0.346312114104146 1.42081328137217e-05 miRanda -0.12756767272718 0.000455424352559313 NA NA NA hsa-miR-491-5p TP53 -0.749881113074983 8.22859263179194e-09 0.346312114104146 1.42081328137217e-05 MirTarget;miRanda -0.210461133541589 1.59624493209635e-19 23519249 Targeted site prediction indicated that both Bcl-XL and TP53 contain miR-491-5p recognizing sites in their 3' UTRs; Overexpression of miR-491-5p in the pancreatic cancer cell line SW1990 effectively inhibited both endogenous Bcl-XL and TP53 gene expressions pancreatic cancer hsa-miR-504-5p TP53 -0.510726779625117 6.12122269907368e-05 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;MirTarget -0.175599590135581 1.78517568542536e-12 25015107 TFF1 activates p53 through down regulation of miR 504 in gastric cancer; Alternatively we found that the reconstitution of TFF1 down-regulates miR-504 a negative regulator of p53; Western blot analysis data demonstrated that miR-504 abrogates TFF1-induced p53 protein expression and activity; In conclusion the in vitro and in vivo data demonstrate for the first time a novel mechanism by which the tumor suppressor functions of TFF1 involve activation of p53 through down-regulation of miR-504 gastric cancer hsa-miR-504-5p TP53I3 -0.510726779625117 6.12122269907368e-05 0.649356716842886 6.54586153445078e-14 miRNAWalker2_validate -0.199427220318157 1.07036313551481e-11 NA NA NA hsa-miR-1224-5p TP73 -0.53941068839383 0.00201058335769201 0.964090476895583 5.59044169933368e-09 mirMAP -0.11210415403837 0.00781111483262794 NA NA NA hsa-miR-138-5p TP73 -0.616087649920851 1.35200027885729e-05 0.964090476895583 5.59044169933368e-09 MirTarget -0.234483508402263 5.94793628771871e-06 NA NA NA hsa-miR-488-5p TP73 -0.385755602369049 7.99431673275965e-08 0.964090476895583 5.59044169933368e-09 mirMAP -0.391543079285817 0.000231458550339123 NA NA NA hsa-let-7a-3p ZMAT3 0.0530563276543763 0.370749834463566 0.024562456397323 0.769773882221632 mirMAP -0.268635103910218 6.89649562555374e-05 NA NA NA hsa-miR-106b-5p ZMAT3 0.167096183547615 0.0497544396383451 0.024562456397323 0.769773882221632 mirMAP -0.170261251754868 0.000347796943773406 NA NA NA hsa-miR-148b-5p ZMAT3 0.127111784487801 0.188731110216917 0.024562456397323 0.769773882221632 MirTarget -0.188651573382066 4.19832788340146e-07 NA NA NA hsa-miR-17-5p ZMAT3 0.0975751369167082 0.20621110214601 0.024562456397323 0.769773882221632 mirMAP -0.269560012856129 2.51985706064932e-06 NA NA NA hsa-miR-186-5p ZMAT3 0.0606381894130505 0.514217717592464 0.024562456397323 0.769773882221632 MirTarget -0.39230566376434 8.1890133882599e-22 NA NA NA hsa-miR-19a-3p ZMAT3 0.0466707843731911 0.576818938277755 0.024562456397323 0.769773882221632 MirTarget -0.238423459070755 8.3376820194036e-08 NA NA NA hsa-miR-19b-3p ZMAT3 -0.0138427450847924 0.856506310673067 0.024562456397323 0.769773882221632 MirTarget -0.290585314157867 4.15537287267186e-08 NA NA NA hsa-miR-200c-3p ZMAT3 0.413752622631695 8.6936333715621e-06 0.024562456397323 0.769773882221632 MirTarget -0.205959563384138 5.98026007971345e-08 NA NA NA hsa-miR-20a-5p ZMAT3 -0.0115865752288853 0.887069448776018 0.024562456397323 0.769773882221632 mirMAP -0.187697922877071 0.000370175103763504 NA NA NA hsa-miR-28-3p ZMAT3 0.139444423971018 0.0811187936164314 0.024562456397323 0.769773882221632 mirMAP -0.166583277124753 0.00415293126059693 NA NA NA hsa-miR-301a-3p ZMAT3 0.00956875714542704 0.916380325220259 0.024562456397323 0.769773882221632 MirTarget -0.424405298491792 1.82229351939905e-27 NA NA NA hsa-miR-3065-5p ZMAT3 -0.262608140481507 0.0414570974148034 0.024562456397323 0.769773882221632 MirTarget;mirMAP -0.274255600387842 7.59211137248196e-25 NA NA NA hsa-miR-362-5p ZMAT3 -0.0730185557229279 0.425572997292224 0.024562456397323 0.769773882221632 mirMAP -0.2490726836783 2.15998763269918e-10 NA NA NA hsa-miR-374a-3p ZMAT3 0.176226170437625 0.0343958848425446 0.024562456397323 0.769773882221632 MirTarget;miRNATAP -0.323079558164232 1.06744413692063e-09 NA NA NA hsa-miR-374a-5p ZMAT3 0.0121037060909277 0.865417963345881 0.024562456397323 0.769773882221632 mirMAP -0.211005742573199 9.28969321286914e-05 NA NA NA hsa-miR-374b-5p ZMAT3 -0.210113949579608 0.0056866083818614 0.024562456397323 0.769773882221632 mirMAP -0.255146441503145 1.56820897988266e-06 NA NA NA hsa-miR-421 ZMAT3 -0.171573235029879 0.0518112933651029 0.024562456397323 0.769773882221632 mirMAP;miRNATAP -0.17526838764659 1.56788997277325e-05 NA NA NA hsa-miR-423-5p ZMAT3 0.0965587900813789 0.148039180704338 0.024562456397323 0.769773882221632 miRNATAP -0.158144442873344 0.00904103042078743 NA NA NA hsa-miR-425-5p ZMAT3 -0.0412837711174561 0.682284485592858 0.024562456397323 0.769773882221632 MirTarget -0.176134134642428 2.5537963152098e-06 NA NA NA hsa-miR-454-3p ZMAT3 0.177318549827649 0.0120865170334418 0.024562456397323 0.769773882221632 MirTarget -0.289974513956204 3.1310801238371e-08 NA NA NA hsa-miR-576-5p ZMAT3 0.106482276040047 0.194379152564166 0.024562456397323 0.769773882221632 MirTarget -0.394453462406171 2.57024185824139e-20 NA NA NA hsa-miR-590-3p ZMAT3 0.312951515438396 0.000480455423843996 0.024562456397323 0.769773882221632 mirMAP;miRNATAP -0.185659210532145 2.87159601528353e-06 NA NA NA hsa-miR-590-5p ZMAT3 0.16946027035949 0.0386004382081678 0.024562456397323 0.769773882221632 miRanda -0.173650033243766 8.21944678193922e-05 NA NA NA hsa-miR-708-3p ZMAT3 0.00458267260363954 0.962455132950708 0.024562456397323 0.769773882221632 mirMAP -0.219595387287916 6.96743046490771e-08 NA NA NA hsa-miR-9-3p ZMAT3 -0.184945845984695 0.0290051908009009 0.024562456397323 0.769773882221632 MirTarget;mirMAP -0.172450169123374 0.00272253197713594 NA NA NA hsa-miR-93-5p ZMAT3 0.139766237971122 0.101057320076629 0.024562456397323 0.769773882221632 mirMAP -0.414475211839477 8.5577839400403e-11 NA NA NA