miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-23a-3p APAF1 0.111958289789488 0.393086414480463 0.0527330819081095 0.628296064246439 miRNATAP -0.131163580903016 0.000690306154175996 24992592;24249161 Luciferase assay was performed to verify a putative target site of miR-23a in the 3'-UTR of apoptosis protease activating factor 1 APAF1 mRNA; The expression levels of miR-23a and APAF1 in CRC cell lines SW480 and SW620 and clinical samples were assessed using reverse transcription-quantitative real-time PCR RT-qPCR and Western blot; Moreover miR-23a up-regulation was coupled with APAF1 down-regulation in CRC tissue samples;We found that the expression of miR-23a was increased and the level of apoptosis-activating factor-1 APAF-1 was decreased in 5-FU-treated colon cancer cells compared to untreated cells; APAF-1 as a target gene of miR-23a was identified and miR-23a antisense-induced increase in the activation of caspase-9 was observed ; colorectal cancer;colorectal cancer hsa-miR-23b-3p APAF1 -0.070273068040148 0.620585267977425 0.0527330819081095 0.628296064246439 miRNATAP -0.109314526987903 0.00215543348816896 NA NA NA hsa-miR-27a-3p APAF1 0.433402568013802 0.00737164631768323 0.0527330819081095 0.628296064246439 miRNATAP -0.16468480576707 8.19227183327682e-08 NA NA NA hsa-miR-27b-3p APAF1 0.236438984240408 0.12263510345883 0.0527330819081095 0.628296064246439 miRNATAP -0.166392707369462 3.36486831782177e-07 NA NA NA hsa-miR-664a-3p APAF1 0.443077463053857 0.0214248925850984 0.0527330819081095 0.628296064246439 mirMAP -0.111814656754016 1.51855061714124e-05 NA NA NA hsa-miR-664a-5p ATR -0.0936480603578111 0.662274905077117 0.550683780115255 1.77066789898484e-06 mirMAP -0.100350035287241 9.0707123785835e-05 NA NA NA hsa-miR-143-3p BBC3 -1.21021691908494 5.70938667332876e-06 0.729914396380796 0.000240595565409828 miRNATAP -0.259215465787601 6.42589215480081e-15 NA NA NA hsa-miR-423-5p BBC3 -1.80309342242719 1.34791344187441e-19 0.729914396380796 0.000240595565409828 PITA -0.188981076908296 1.9768046193777e-05 NA NA NA hsa-miR-139-5p CASP3 -2.26625553116965 7.22375119369519e-16 0.749868979708077 8.30799302647668e-11 miRanda -0.103400450602475 1.92824413318983e-08 NA NA NA hsa-miR-195-3p CASP3 -1.33068822339418 6.65427393047357e-09 0.749868979708077 8.30799302647668e-11 MirTarget -0.12156615230384 1.38560895801409e-07 NA NA NA hsa-miR-30c-5p CASP3 -0.327319105755825 0.123595832152687 0.749868979708077 8.30799302647668e-11 miRNATAP -0.14954468041522 2.8553699558622e-09 NA NA NA hsa-miR-30d-5p CASP3 -0.920901858717441 3.50898334321668e-05 0.749868979708077 8.30799302647668e-11 miRNAWalker2_validate;miRTarBase;miRNATAP -0.173377620122409 2.06657661969359e-13 NA NA NA hsa-let-7b-5p CCNB1 -1.62016148171663 1.56547487335694e-15 2.58605326822805 1.49721003431101e-20 miRNAWalker2_validate -0.600740382615842 9.27053199216893e-23 NA NA NA hsa-miR-139-5p CCNB1 -2.26625553116965 7.22375119369519e-16 2.58605326822805 1.49721003431101e-20 miRanda -0.399807636878469 2.18106497381391e-19 NA NA NA hsa-let-7a-5p CCNB2 -1.37014061604018 3.11451492971224e-14 3.04708109169996 5.83056043976083e-23 miRNAWalker2_validate -0.568312373812622 7.0365010069257e-13 NA NA NA hsa-let-7b-5p CCNB2 -1.62016148171663 1.56547487335694e-15 3.04708109169996 5.83056043976083e-23 miRNAWalker2_validate -0.613229739107824 5.61658809656701e-19 NA NA NA hsa-let-7c-5p CCNB2 -2.14139413005852 1.66416999341685e-18 3.04708109169996 5.83056043976083e-23 miRNAWalker2_validate -0.617345582596585 1.15760664423345e-28 NA NA NA hsa-miR-23b-3p CCNB2 -0.070273068040148 0.620585267977425 3.04708109169996 5.83056043976083e-23 miRNAWalker2_validate -0.601133570738828 1.22608993636691e-08 NA NA NA hsa-miR-106a-5p CCND1 1.3902608059488 6.07931522284525e-05 -0.295767629587253 0.255400743295204 MirTarget;miRNATAP -0.247441760563832 4.58943715400631e-11 NA NA NA hsa-miR-106b-5p CCND1 1.47447672652218 1.32004094930462e-13 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.261384817974007 7.4979419817232e-06 NA NA NA hsa-miR-142-3p CCND1 3.98476449801341 1.56256182021111e-35 -0.295767629587253 0.255400743295204 miRanda -0.119299030581201 0.000532277158339159 23619912 Transfection of miR-142-3p mimics in colon cancer cells downregulated cyclin D1 expression induced G1 phase cell cycle arrest and elevated the sensitivity of the cells to 5-fluorouracil colon cancer hsa-miR-15a-5p CCND1 1.62575353599172 1.55312121019827e-19 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.199069637483131 0.0019294787514794 22922827 CCND1 has been found to be a target of miR-15a and miR-16-1 through analysis of complementary sequences between microRNAs and CCND1 mRNA; Moreover the transcription of CCND1 is suppressed by miR-15a and miR-16-1 via direct binding to the CCND1 3'-untranslated region 3'-UTR sarcoma hsa-miR-15b-5p CCND1 -1.25935038177398 1.83460220856901e-12 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.303824360423629 3.25741318048184e-06 NA NA NA hsa-miR-16-1-3p CCND1 1.50034693398929 6.63958242991125e-10 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase -0.249107447749185 3.23141967346606e-05 22922827;18483394 CCND1 has been found to be a target of miR-15a and miR-16-1 through analysis of complementary sequences between microRNAs and CCND1 mRNA; Moreover the transcription of CCND1 is suppressed by miR-15a and miR-16-1 via direct binding to the CCND1 3'-untranslated region 3'-UTR;Truncation in CCND1 mRNA alters miR 16 1 regulation in mantle cell lymphoma; Furthermore we demonstrated that this truncation alters miR-16-1 binding sites and through the use of reporter constructs we were able to show that miR-16-1 regulates CCND1 mRNA expression; This study introduces the role of miR-16-1 in the regulation of CCND1 in MCL ; sarcoma;lymphoma hsa-miR-16-5p CCND1 0.749619098384047 4.11649450706988e-06 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.371885624299403 3.21606546730418e-07 23991964;22922827;18483394 At the molecular level our results further revealed that cyclin D1 expression was negatively regulated by miR-16;CCND1 has been found to be a target of miR-15a and miR-16-1 through analysis of complementary sequences between microRNAs and CCND1 mRNA; Moreover the transcription of CCND1 is suppressed by miR-15a and miR-16-1 via direct binding to the CCND1 3'-untranslated region 3'-UTR;Truncation in CCND1 mRNA alters miR 16 1 regulation in mantle cell lymphoma; Furthermore we demonstrated that this truncation alters miR-16-1 binding sites and through the use of reporter constructs we were able to show that miR-16-1 regulates CCND1 mRNA expression; This study introduces the role of miR-16-1 in the regulation of CCND1 in MCL ;; bladder cancer;sarcoma;lymphoma hsa-miR-17-5p CCND1 2.07078488653279 2.77403038572568e-19 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.313758634258834 1.59321495552009e-10 26431674 Bioinformatics Prediction and In Vitro Analysis Revealed That miR 17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells; In this study using bioinformatic analyses miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score; After lentiviral transduction of miR-17 to the target cells gene expression analysis showed decreased expression of CCND1 gene breast cancer hsa-miR-186-5p CCND1 0.852175445703945 6.88885738584309e-10 -0.295767629587253 0.255400743295204 mirMAP -0.377477028673122 1.06268066352005e-05 NA NA NA hsa-miR-193a-3p CCND1 0.549173467360291 0.0319030569365466 -0.295767629587253 0.255400743295204 MirTarget;PITA;miRanda -0.190950932146342 0.000161171890671323 NA NA NA hsa-miR-193b-3p CCND1 1.10444771306846 0.000822888533401377 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;MirTarget -0.147999411883792 3.70620495015026e-05 27071318;20655737;20304954;21893020;26129688 MicroRNA 193b inhibits the proliferation migration and invasion of gastric cancer cells via targeting cyclin D1; Further mechanism study indicated that CCND1 was a direct target of miR-193b in GC;CCND1 and ETS1 were revealed to be regulated by miR-193b directly;MicroRNA 193b represses cell proliferation and regulates cyclin D1 in melanoma; Overexpression of miR-193b in Malme-3M cells down-regulated CCND1 mRNA and protein by > or = 50%; A luciferase reporter assay confirmed that miR-193b directly regulates CCND1 by binding to the 3'untranslated region of CCND1 mRNA; These studies indicate that miR-193b represses cell proliferation and regulates CCND1 expression and suggest that dysregulation of miR-193b may play an important role in melanoma development;In a previous study we reported that miR-193b represses cell proliferation and regulates cyclin D1 in melanoma cells suggesting that miR-193b could act as a tumor suppressor;Epigenetically altered miR 193b targets cyclin D1 in prostate cancer; It has been suggested that miR-193b targets cyclin D1 in several malignancies; Here our aim was to determine if miR-193b targets cyclin D1 in prostate cancer; Furthermore the PC cell lines 22Rv1 and VCaP which express low levels of miR-193b and high levels of CCND1 showed significant growth retardation when treated with a CDK4/6 inhibitor; In contrast the inhibitor had no effect on the growth of PC-3 and DU145 cells with high miR-193b and low CCND1 expression; Taken together our data demonstrate that miR-193b targets cyclin D1 in prostate cancer ;;;; gastric cancer;liver cancer;melanoma;melanoma;prostate cancer hsa-miR-19a-3p CCND1 2.11776595911517 7.07663078397156e-10 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;miRNATAP -0.21539529985992 9.86203433305448e-08 25985117 Moreover miR-19a might play inhibitory roles in HCC malignancy via regulating Cyclin D1 expression liver cancer hsa-miR-19b-1-5p CCND1 1.71217036351784 3.28561659177495e-12 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase -0.301713494105536 2.89182846291565e-08 NA NA NA hsa-miR-19b-3p CCND1 2.10868505662119 5.40846613768389e-17 -0.295767629587253 0.255400743295204 miRNATAP -0.179773518063806 0.000197856717032667 NA NA NA hsa-miR-20a-5p CCND1 2.64649625616975 5.1469418982085e-27 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.237160136465301 1.84669386058676e-07 NA NA NA hsa-miR-20b-5p CCND1 1.35770573270947 0.00260808530013016 -0.295767629587253 0.255400743295204 MirTarget;miRNATAP -0.179107595068828 5.65966117118738e-11 NA NA NA hsa-miR-365a-3p CCND1 0.0132777124336201 0.953597412537431 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;miRTarBase -0.193166924738263 0.000234492957903034 NA NA NA hsa-miR-374a-5p CCND1 -0.197027254874826 0.298080902192861 -0.295767629587253 0.255400743295204 MirTarget -0.313785702713176 9.54081268797886e-06 27191497 microRNA 374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway; Furthermore luciferase reporter assays confirmed that miR-374a could directly reduce CCND1; We examined miR-374a levels by in situ hybridization and its correlation with CCND1 expression in CRC tumor tissues; High miR-374a expression with low level of CCND1 was protective factor in CRC; Together these findings indicate that miR-374a inactivates the PI3K/AKT axis by inhibiting CCND1 suppressing of colon cancer progression progression colon cancer hsa-miR-374b-5p CCND1 0.474691035780475 0.0109217471394203 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;MirTarget -0.26116225100222 0.00011561978488339 NA NA NA hsa-miR-425-5p CCND1 1.21659524959201 8.01126418494715e-09 -0.295767629587253 0.255400743295204 miRNAWalker2_validate -0.245575402525843 1.03844251357893e-05 NA NA NA hsa-miR-589-3p CCND1 1.34253876396688 1.84935927493927e-05 -0.295767629587253 0.255400743295204 MirTarget -0.130907472589194 0.000793150025378685 NA NA NA hsa-miR-590-3p CCND1 0.83851360565783 0.00129224433453587 -0.295767629587253 0.255400743295204 mirMAP -0.182941292569374 0.000856336828554768 NA NA NA hsa-miR-769-3p CCND1 0.452126659761753 0.0748150639143322 -0.295767629587253 0.255400743295204 mirMAP -0.142316943866129 0.00276805281261241 NA NA NA hsa-miR-92a-3p CCND1 -0.136362762339122 0.493412263619364 -0.295767629587253 0.255400743295204 miRNAWalker2_validate -0.337251172229938 1.62058605849004e-08 NA NA NA hsa-miR-93-5p CCND1 1.50844106515467 1.83836866996875e-12 -0.295767629587253 0.255400743295204 miRNAWalker2_validate;MirTarget;miRNATAP -0.320397820083252 2.84014735439528e-09 NA NA NA hsa-miR-942-5p CCND1 -0.0372813055050076 0.870631058767894 -0.295767629587253 0.255400743295204 MirTarget -0.177314558661089 0.000979800060419363 NA NA NA hsa-miR-106a-5p CCND2 1.3902608059488 6.07931522284525e-05 -1.64140990579885 1.49666938681079e-12 miRNATAP -0.149132151233025 1.88464236882735e-05 NA NA NA hsa-miR-106b-5p CCND2 1.47447672652218 1.32004094930462e-13 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate;miRTarBase;miRNATAP -0.383655587509124 2.65429916887189e-13 NA NA NA hsa-miR-130b-5p CCND2 1.53645187062942 1.10498816261505e-08 -1.64140990579885 1.49666938681079e-12 mirMAP -0.268032769997331 9.22343432530747e-12 NA NA NA hsa-miR-141-3p CCND2 3.36993755486106 5.42939251147195e-32 -1.64140990579885 1.49666938681079e-12 MirTarget;TargetScan -0.239914655059016 6.4654039095017e-12 NA NA NA hsa-miR-151a-3p CCND2 0.665271883054405 0.000281673348137154 -1.64140990579885 1.49666938681079e-12 mirMAP -0.279142680983949 2.60564778780149e-06 NA NA NA hsa-miR-15a-5p CCND2 1.62575353599172 1.55312121019827e-19 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate;miRTarBase;miRNATAP -0.158338209854594 0.00711668272045409 NA NA NA hsa-miR-17-5p CCND2 2.07078488653279 2.77403038572568e-19 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.365048259055278 1.61934056872758e-16 NA NA NA hsa-miR-182-5p CCND2 3.21839823004635 5.13049145201115e-39 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate;miRTarBase;miRNATAP -0.373145719566719 1.32597677460149e-21 NA NA NA hsa-miR-183-5p CCND2 2.39247234736657 3.92444525331999e-21 -1.64140990579885 1.49666938681079e-12 miRNATAP -0.410520271082553 1.11291256348519e-25 NA NA NA hsa-miR-185-5p CCND2 1.14291034858482 2.95777119743491e-13 -1.64140990579885 1.49666938681079e-12 MirTarget;miRNATAP -0.23940712387784 0.000484175543966546 NA NA NA hsa-miR-186-5p CCND2 0.852175445703945 6.88885738584309e-10 -1.64140990579885 1.49666938681079e-12 mirMAP;miRNATAP -0.357370184588874 5.14493635358813e-06 NA NA NA hsa-miR-191-5p CCND2 0.343645878547116 0.0668114086143391 -1.64140990579885 1.49666938681079e-12 MirTarget -0.19767765908675 0.000750401585158583 NA NA NA hsa-miR-19a-3p CCND2 2.11776595911517 7.07663078397156e-10 -1.64140990579885 1.49666938681079e-12 MirTarget;miRNATAP -0.167455065978335 6.56621464464812e-06 NA NA NA hsa-miR-19b-3p CCND2 2.10868505662119 5.40846613768389e-17 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate;MirTarget;miRNATAP -0.19157786848984 1.39328108679414e-05 NA NA NA hsa-miR-200a-3p CCND2 3.14766878548496 1.32850889777422e-21 -1.64140990579885 1.49666938681079e-12 MirTarget -0.173744823500602 6.53232158053391e-08 NA NA NA hsa-miR-20a-5p CCND2 2.64649625616975 5.1469418982085e-27 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate;miRTarBase;miRNATAP -0.28367508352758 5.6750334332974e-12 NA NA NA hsa-miR-21-3p CCND2 2.54206008030157 1.11598785398763e-30 -1.64140990579885 1.49666938681079e-12 mirMAP -0.212907340098529 4.00646631535515e-06 NA NA NA hsa-miR-2355-3p CCND2 1.10676344164715 6.24068383465151e-06 -1.64140990579885 1.49666938681079e-12 miRNATAP -0.221166273655799 1.96123873319489e-06 NA NA NA hsa-miR-28-5p CCND2 1.19639196514745 4.59365801666536e-18 -1.64140990579885 1.49666938681079e-12 miRanda -0.289410618824274 0.00017592467355866 NA NA NA hsa-miR-301a-3p CCND2 2.70198002906399 1.56420889710984e-14 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate -0.265639128307335 3.9945374923581e-13 NA NA NA hsa-miR-320b CCND2 0.226640952564488 0.378818692487242 -1.64140990579885 1.49666938681079e-12 mirMAP;miRNATAP -0.123605256651271 0.00373003525561283 NA NA NA hsa-miR-324-3p CCND2 -0.0774700133118458 0.689226297032231 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate -0.276159423764137 9.9462768745637e-07 NA NA NA hsa-miR-331-5p CCND2 0.578237334664505 0.00131211239210882 -1.64140990579885 1.49666938681079e-12 miRNATAP -0.275952758002927 9.36107182529594e-06 NA NA NA hsa-miR-429 CCND2 2.3794516574089 1.72813419703734e-13 -1.64140990579885 1.49666938681079e-12 miRNATAP -0.209322906628109 2.95963655497587e-09 NA NA NA hsa-miR-450b-5p CCND2 1.69248953402469 1.96052058003353e-07 -1.64140990579885 1.49666938681079e-12 MirTarget;PITA;miRNATAP -0.145422700655101 8.53356735526403e-05 NA NA NA hsa-miR-501-5p CCND2 0.411357595674671 0.10435288464149 -1.64140990579885 1.49666938681079e-12 PITA;mirMAP;miRNATAP -0.144149908130612 0.000856140982438327 NA NA NA hsa-miR-503-5p CCND2 1.97129438815516 2.1704434283035e-09 -1.64140990579885 1.49666938681079e-12 MirTarget -0.132569636435255 4.69239346000773e-05 25860935 We then identified two targets of miR-503 CCND2 and CCND3 breast cancer hsa-miR-550a-5p CCND2 0.59977150019756 0.0314775340172755 -1.64140990579885 1.49666938681079e-12 MirTarget -0.220699899618513 1.33146855439522e-08 NA NA NA hsa-miR-589-3p CCND2 1.34253876396688 1.84935927493927e-05 -1.64140990579885 1.49666938681079e-12 mirMAP -0.194613807523332 3.68596504229146e-08 NA NA NA hsa-miR-590-3p CCND2 0.83851360565783 0.00129224433453587 -1.64140990579885 1.49666938681079e-12 miRanda;mirMAP -0.133052154455084 0.00820830741469702 NA NA NA hsa-miR-590-5p CCND2 2.07441240363458 4.95240551694843e-14 -1.64140990579885 1.49666938681079e-12 mirMAP -0.258740782004531 5.16823341358779e-08 NA NA NA hsa-miR-660-5p CCND2 2.04875198906126 1.08269480928897e-17 -1.64140990579885 1.49666938681079e-12 mirMAP -0.145565210932688 0.00131443056428649 NA NA NA hsa-miR-7-1-3p CCND2 2.61364240402652 1.98959237815786e-32 -1.64140990579885 1.49666938681079e-12 mirMAP -0.259792711431322 7.83559584811719e-08 NA NA NA hsa-miR-877-5p CCND2 -0.370234513208225 0.20670834111988 -1.64140990579885 1.49666938681079e-12 miRNAWalker2_validate -0.150269749089241 5.7135351515868e-05 NA NA NA hsa-miR-93-5p CCND2 1.50844106515467 1.83836866996875e-12 -1.64140990579885 1.49666938681079e-12 miRNATAP -0.414585687663892 1.04746846150745e-17 NA NA NA hsa-miR-96-5p CCND2 3.0381097138467 4.40362891630201e-24 -1.64140990579885 1.49666938681079e-12 TargetScan;miRNATAP -0.357833634781266 1.13611538125112e-20 NA NA NA hsa-miR-320b CCND3 0.226640952564488 0.378818692487242 -0.858556164090156 9.93688486289821e-06 miRanda -0.106740567141186 0.00235090988932412 NA NA NA hsa-miR-421 CCND3 0.170525317701774 0.535277026680289 -0.858556164090156 9.93688486289821e-06 PITA;miRanda -0.120402813664522 0.000730915299268324 NA NA NA hsa-miR-96-5p CCND3 3.0381097138467 4.40362891630201e-24 -0.858556164090156 9.93688486289821e-06 TargetScan -0.12470872406597 0.000146574618288945 NA NA NA hsa-miR-125b-5p CCNE1 -0.548859243919575 0.0107224406584463 2.99717740665025 1.61069658019784e-19 miRNAWalker2_validate -0.212969225547095 0.00407731324503497 NA NA NA hsa-miR-195-5p CCNE1 -1.01577502605386 4.80814718341729e-05 2.99717740665025 1.61069658019784e-19 miRNAWalker2_validate;MirTarget;miRNATAP -0.51804360034979 1.42698078497522e-15 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.126411409001078 0.440028840408711 2.99717740665025 1.61069658019784e-19 miRNAWalker2_validate;miRTarBase;miRNATAP -0.695101497950461 4.5037170303388e-13 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-497-5p CCNE1 -0.0546347378264729 0.786205564901684 2.99717740665025 1.61069658019784e-19 MirTarget;miRNATAP -0.495681038343954 2.32850749141189e-10 25909221;24112607;24909281 The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; lung cancer;breast cancer;cervical and endocervical cancer hsa-let-7a-3p CCNE2 0.167285443861993 0.431834813181487 2.14869777933212 3.82193505863706e-13 mirMAP -0.219072556802333 0.00307083362438944 NA NA NA hsa-let-7b-3p CCNE2 -1.81889978084306 6.9913416210266e-19 2.14869777933212 3.82193505863706e-13 mirMAP -0.490706485648775 1.99669157811241e-14 NA NA NA hsa-miR-126-3p CCNE2 0.397563736477277 0.115641762947829 2.14869777933212 3.82193505863706e-13 miRNAWalker2_validate -0.150847603483629 0.0065596481993981 NA NA NA hsa-miR-26a-5p CCNE2 -0.126411409001078 0.440028840408711 2.14869777933212 3.82193505863706e-13 miRNAWalker2_validate;miRTarBase;miRNATAP -0.509665959363437 2.11729025570925e-09 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-26b-5p CCNE2 0.72362441215247 4.7794659748036e-05 2.14869777933212 3.82193505863706e-13 miRNATAP -0.221903049454022 0.0047653538397741 NA NA NA hsa-miR-3065-5p CCNE2 0.653308707552535 0.0999497219778851 2.14869777933212 3.82193505863706e-13 mirMAP -0.199731594807246 1.35834639539203e-07 NA NA NA hsa-miR-30a-5p CCNE2 -0.924084264430689 0.000762030454634728 2.14869777933212 3.82193505863706e-13 miRNATAP -0.485926886026448 9.30670075166704e-24 NA NA NA hsa-miR-30b-5p CCNE2 0.358144314839612 0.138026229059594 2.14869777933212 3.82193505863706e-13 miRNAWalker2_validate;miRTarBase -0.201650464117881 0.000503320898048745 22384020 A luciferase-based reporter assay demonstrated that miR-30b post-transcriptionally reduced 27% p = 0.005 of the gene expression by interacting with two binding sites in the 3'-UTR of CCNE2; The upregulation of miR-30b by trastuzumab may play a biological role in trastuzumab-induced cell growth inhibition by targeting CCNE2 breast cancer hsa-miR-30c-5p CCNE2 -0.327319105755825 0.123595832152687 2.14869777933212 3.82193505863706e-13 miRNATAP -0.441580113864178 9.21074303399811e-12 NA NA NA hsa-miR-30d-5p CCNE2 -0.920901858717441 3.50898334321668e-05 2.14869777933212 3.82193505863706e-13 miRNATAP -0.348831475795763 1.65705272947657e-08 25843294 MicroRNA 30d 5p inhibits tumour cell proliferation and motility by directly targeting CCNE2 in non small cell lung cancer; In addition the re-introduction of CCNE2 expression antagonised the inhibitory effects of miR-30d-5p on the capacity of NSCLC cells for proliferation and motility motility lung squamous cell cancer hsa-miR-34a-5p CCNE2 1.40588612517769 8.60097477768592e-12 2.14869777933212 3.82193505863706e-13 miRNAWalker2_validate;miRTarBase;miRNATAP -0.195460410714665 0.00336778531948037 NA NA NA hsa-miR-34c-5p CCNE2 -1.00125440032112 0.0724432896090357 2.14869777933212 3.82193505863706e-13 miRNAWalker2_validate;miRTarBase;PITA;miRanda;miRNATAP -0.107375543427311 4.3380723232889e-05 NA NA NA hsa-miR-514a-3p CCNE2 -0.62130231811134 0.313738530078239 2.14869777933212 3.82193505863706e-13 miRNATAP -0.174092237043764 3.53454317603187e-12 NA NA NA hsa-miR-132-3p CCNG1 0.0122601212105202 0.934032846062939 -0.381998946658134 0.021474550007251 MirTarget -0.162870587393475 0.00184944520398931 NA NA NA hsa-miR-197-3p CCNG1 -1.2979654860824 1.79649132641813e-07 -0.381998946658134 0.021474550007251 miRNAWalker2_validate -0.121001122524791 6.45126455052382e-05 NA NA NA hsa-miR-21-5p CCNG1 4.38165933891779 1.52536633902505e-92 -0.381998946658134 0.021474550007251 miRNAWalker2_validate -0.127417479828893 1.0695641766096e-05 NA NA NA hsa-miR-23a-3p CCNG1 0.111958289789488 0.393086414480463 -0.381998946658134 0.021474550007251 MirTarget;miRNATAP -0.183198674467916 0.00202799417869928 NA NA NA hsa-miR-27a-3p CCNG1 0.433402568013802 0.00737164631768323 -0.381998946658134 0.021474550007251 MirTarget;miRNATAP -0.25375430158064 7.1666208486228e-08 NA NA NA hsa-miR-27b-3p CCNG1 0.236438984240408 0.12263510345883 -0.381998946658134 0.021474550007251 MirTarget;miRNATAP -0.172140886867638 0.000639941826798135 26623719 Moreover miR-27b directly targets the 3' untranslated regions 3'-UTRs of CCNG1 a well-known negative regulator of P53 stability; Interestingly miR-27b up-regulation leads to increased miR-508-5p expression and this phenomenon is mediated by CCNG1 and P53 gastric cancer hsa-miR-339-5p CCNG1 0.536997313601114 0.0488090394176611 -0.381998946658134 0.021474550007251 miRanda -0.153697962249595 2.98553989475299e-08 NA NA NA hsa-miR-590-5p CCNG1 2.07441240363458 4.95240551694843e-14 -0.381998946658134 0.021474550007251 miRanda -0.18919609301239 1.12937006224063e-08 NA NA NA hsa-miR-96-5p CCNG1 3.0381097138467 4.40362891630201e-24 -0.381998946658134 0.021474550007251 MirTarget;TargetScan -0.125986304075834 5.6639500582943e-06 NA NA NA hsa-miR-195-3p CCNG2 -1.33068822339418 6.65427393047357e-09 0.220278413829613 0.10547582961774 mirMAP -0.102425950333312 0.000136909940566372 NA NA NA hsa-miR-365a-3p CCNG2 0.0132777124336201 0.953597412537431 0.220278413829613 0.10547582961774 MirTarget -0.113516970672079 3.36006603968573e-05 NA NA NA hsa-miR-374a-5p CCNG2 -0.197027254874826 0.298080902192861 0.220278413829613 0.10547582961774 mirMAP -0.137445535309871 0.000214127784957003 NA NA NA hsa-miR-664a-3p CCNG2 0.443077463053857 0.0214248925850984 0.220278413829613 0.10547582961774 MirTarget;mirMAP -0.160018466299354 7.23956577877032e-07 NA NA NA hsa-miR-23b-3p CDK2 -0.070273068040148 0.620585267977425 0.335081436185588 0.0196934506015732 miRNAWalker2_validate -0.320796841599285 4.19225271403267e-12 NA NA NA hsa-miR-26b-5p CDK2 0.72362441215247 4.7794659748036e-05 0.335081436185588 0.0196934506015732 miRNAWalker2_validate -0.127461620300032 0.000584290801690183 NA NA NA hsa-miR-145-5p CDK4 -1.34926078156437 2.63526538800669e-07 0.737437814725925 6.46735685355202e-06 miRNAWalker2_validate;miRTarBase -0.151032894863878 9.5680433026529e-08 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-195-5p CDK4 -1.01577502605386 4.80814718341729e-05 0.737437814725925 6.46735685355202e-06 miRNAWalker2_validate;miRTarBase -0.113117227761123 0.000369485847021713 NA NA NA hsa-miR-320a CDK4 -0.961144925954715 1.62478794429204e-08 0.737437814725925 6.46735685355202e-06 miRNAWalker2_validate -0.198117291941419 6.62226154091084e-06 NA NA NA hsa-miR-141-3p CDK6 3.36993755486106 5.42939251147195e-32 -0.634679756888932 0.0174689602657709 TargetScan;miRNATAP -0.160612180698081 5.76268904101641e-05 NA NA NA hsa-miR-148a-3p CDK6 2.30885799126944 1.95226081368985e-21 -0.634679756888932 0.0174689602657709 mirMAP -0.140512406763982 0.00377970382659687 NA NA NA hsa-miR-15a-5p CDK6 1.62575353599172 1.55312121019827e-19 -0.634679756888932 0.0174689602657709 miRNATAP -0.213028438155036 0.00125907527826487 NA NA NA hsa-miR-16-1-3p CDK6 1.50034693398929 6.63958242991125e-10 -0.634679756888932 0.0174689602657709 mirMAP -0.187334541166986 0.00249778145407185 NA NA NA hsa-miR-182-5p CDK6 3.21839823004635 5.13049145201115e-39 -0.634679756888932 0.0174689602657709 mirMAP -0.195465673682201 1.86720454693084e-05 NA NA NA hsa-miR-200a-3p CDK6 3.14766878548496 1.32850889777422e-21 -0.634679756888932 0.0174689602657709 miRNATAP -0.172932973424539 1.8796595520804e-06 24009066 microRNA 200a is an independent prognostic factor of hepatocellular carcinoma and induces cell cycle arrest by targeting CDK6 liver cancer hsa-miR-200b-3p CDK6 1.55157578247391 4.83790020897188e-09 -0.634679756888932 0.0174689602657709 mirMAP -0.168627583367613 0.000217208517169461 NA NA NA hsa-miR-21-5p CDK6 4.38165933891779 1.52536633902505e-92 -0.634679756888932 0.0174689602657709 miRNAWalker2_validate;mirMAP -0.148024006269382 0.00157509032784336 NA NA NA hsa-miR-23b-3p CDK6 -0.070273068040148 0.620585267977425 -0.634679756888932 0.0174689602657709 mirMAP -0.23876595315757 0.0067366952470222 NA NA NA hsa-miR-27b-3p CDK6 0.236438984240408 0.12263510345883 -0.634679756888932 0.0174689602657709 mirMAP -0.290275286674932 0.000346874480244186 NA NA NA hsa-miR-27b-5p CDK6 1.03511957999268 7.86187917705303e-11 -0.634679756888932 0.0174689602657709 mirMAP -0.218863599231384 0.00422822542967776 NA NA NA hsa-miR-29b-3p CDK6 3.10527711861309 3.01242507282711e-32 -0.634679756888932 0.0174689602657709 miRNAWalker2_validate;miRTarBase;miRNATAP -0.177579122887668 4.47727335928492e-05 23591808;23245396;25472644;26180082;27230400;20086245 Here we have identified the oncogene cyclin-dependent protein kinase 6 CDK6 as a direct target of miR-29b in lung cancer;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;Moreover miR-29b inhibited the expression of MCL1 and CDK6;Knockdown of NTSR1 increased the expression of miR-29b-1 and miR-129-3p which were responsible for the decreased CDK6 expression;MiR 29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6; In this study we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods; We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells; Furthermore we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues; The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL ;;;;; lung cancer;melanoma;colorectal cancer;glioblastoma;sarcoma;lymphoma hsa-miR-3065-5p CDK6 0.653308707552535 0.0999497219778851 -0.634679756888932 0.0174689602657709 mirMAP -0.22642349631217 6.97387440613112e-12 NA NA NA hsa-miR-30b-5p CDK6 0.358144314839612 0.138026229059594 -0.634679756888932 0.0174689602657709 mirMAP -0.297003306946081 3.8128721932856e-09 NA NA NA hsa-miR-30c-5p CDK6 -0.327319105755825 0.123595832152687 -0.634679756888932 0.0174689602657709 mirMAP -0.164968126318733 0.0045742419018014 NA NA NA hsa-miR-30d-3p CDK6 0.00396376345349925 0.986457040013182 -0.634679756888932 0.0174689602657709 mirMAP -0.371507952440074 1.92927097384112e-10 NA NA NA hsa-miR-30d-5p CDK6 -0.920901858717441 3.50898334321668e-05 -0.634679756888932 0.0174689602657709 mirMAP -0.319235369499537 4.17247463199845e-09 NA NA NA hsa-miR-30e-5p CDK6 1.60414100786743 3.78951090573667e-19 -0.634679756888932 0.0174689602657709 mirMAP -0.286452484165894 1.41651230242754e-05 NA NA NA hsa-miR-338-3p CDK6 0.726843067232002 0.0506251324215494 -0.634679756888932 0.0174689602657709 mirMAP -0.154832977052286 2.36104508154558e-06 NA NA NA hsa-miR-34a-5p CDK6 1.40588612517769 8.60097477768592e-12 -0.634679756888932 0.0174689602657709 miRNAWalker2_validate;miRTarBase;miRNATAP -0.243763697376835 2.99239665927089e-05 26104764;21702042 The expression of microRNA 34a is inversely correlated with c MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients; We found significant inverse correlations between miR-34a and c-MET R = -0.316 P = 0.028 and CDK6 expression R = -0.4582 P = 0.004;Molecular analyses identified Cdk6 and sirtuin SIRT-1 as being targeted by miR-34a in MI-TCC cells however inhibition of Cdk6 and SIRT-1 was not as effective as pre-miR-34a in mediating chemosensitization ; lung cancer;bladder cancer hsa-miR-362-5p CDK6 0.658947634569393 0.0243306847205756 -0.634679756888932 0.0174689602657709 mirMAP -0.182028148769008 2.22030118459272e-05 NA NA NA hsa-miR-429 CDK6 2.3794516574089 1.72813419703734e-13 -0.634679756888932 0.0174689602657709 mirMAP;miRNATAP -0.15019667316567 0.000181432042876184 NA NA NA hsa-miR-660-5p CDK6 2.04875198906126 1.08269480928897e-17 -0.634679756888932 0.0174689602657709 mirMAP -0.160480938026846 0.00163906606751145 NA NA NA hsa-miR-664a-3p CDK6 0.443077463053857 0.0214248925850984 -0.634679756888932 0.0174689602657709 mirMAP -0.300162344123568 2.48177468084385e-06 NA NA NA hsa-miR-106b-5p CDKN1A 1.47447672652218 1.32004094930462e-13 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.227776235142808 2.11586866823657e-07 NA NA NA hsa-miR-17-5p CDKN1A 2.07078488653279 2.77403038572568e-19 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.155626227970113 3.32801748379634e-05 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-182-5p CDKN1A 3.21839823004635 5.13049145201115e-39 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate -0.191273978004266 8.63402331622284e-09 NA NA NA hsa-miR-20a-5p CDKN1A 2.64649625616975 5.1469418982085e-27 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.137674450234877 6.74026700667366e-05 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-28-5p CDKN1A 1.19639196514745 4.59365801666536e-18 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRanda;miRNATAP -0.202389894871562 0.0015056884290614 NA NA NA hsa-miR-93-5p CDKN1A 1.50844106515467 1.83836866996875e-12 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.165683989353739 5.62354734150849e-05 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-96-5p CDKN1A 3.0381097138467 4.40362891630201e-24 -0.989660104023605 3.33169106596478e-07 miRNAWalker2_validate;miRTarBase -0.146610096725374 7.80340952179366e-06 26582573 Upregulation of microRNA 96 and its oncogenic functions by targeting CDKN1A in bladder cancer; Bioinformatics prediction combined with luciferase reporter assay were used to verify whether the cyclin-dependent kinase inhibitor CDKN1A was a potential target gene of miR-96; According to the data of miRTarBase CDKN1A might be a candidate target gene of miR-96; In addition luciferase reporter and Western blot assays respectively demonstrated that miR-96 could bind to the putative seed region in CDKN1A mRNA 3'UTR and significantly reduce the expression level of CDKN1A protein; Moreover we found that the inhibition of miR-96 expression remarkably decreased cell proliferation and promoted cell apoptosis of BC cell lines which was consistent with the findings observed following the introduction of CDKN1A cDNA without 3'UTR restored miR-96; Upregulation of miR-96 may contribute to aggressive malignancy partly through suppressing CDKN1A protein expression in BC cells bladder cancer hsa-miR-215-5p CDKN2A 0.116505586532384 0.8384722675454 1.92308660588306 0.00061855049856206 miRNAWalker2_validate -0.177578968663255 0.000710634851332846 NA NA NA hsa-miR-139-5p CHEK1 -2.26625553116965 7.22375119369519e-16 1.85259564745725 8.22396650402656e-14 miRanda -0.332742769048999 1.86178611104622e-17 NA NA NA hsa-miR-195-5p CHEK1 -1.01577502605386 4.80814718341729e-05 1.85259564745725 8.22396650402656e-14 MirTarget;miRNATAP -0.345574150233039 7.39222165825211e-13 25840419 MiR 195 suppresses non small cell lung cancer by targeting CHEK1; We discovered that CHEK1 was a direct target of miR-195 which decreased CHEK1 expression in lung cancer cells lung squamous cell cancer hsa-miR-320a CHEK1 -0.961144925954715 1.62478794429204e-08 1.85259564745725 8.22396650402656e-14 MirTarget;miRanda -0.279842886792326 4.08338178278489e-05 NA NA NA hsa-miR-326 CHEK1 -0.992397386750901 0.00335173765975096 1.85259564745725 8.22396650402656e-14 miRanda -0.121601688728903 0.000425451688060438 NA NA NA hsa-miR-497-5p CHEK1 -0.0546347378264729 0.786205564901684 1.85259564745725 8.22396650402656e-14 MirTarget;miRNATAP -0.487503389798205 7.93376609427637e-18 24464213 Checkpoint kinase 1 is negatively regulated by miR 497 in hepatocellular carcinoma; In silico analysis showed that CHEK1 was a candidate target of miR-497 which was previously found to be downregulated in HCC by us; To test whether miR-497 could bind to 3'untranslated region 3'UTR of CHEK1 luciferase reporter assay was conducted; The result revealed that miR-497 could bind to the 3'untranslated region 3'UTR of CHEK1 mRNA; Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1; Finally miR-497 expression was measured in the same 30 HCC samples and the correlation between miR-497 and CHEK1 was analyzed; The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1; Taken together these results demonstrated that CHEK1 was negatively regulated by miR-497 and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC which provided a potential molecular target for HCC therapy liver cancer hsa-miR-139-5p CYCS -2.26625553116965 7.22375119369519e-16 0.533584069273294 0.000797541450763797 miRanda -0.177347725394177 7.68044998537411e-13 NA NA NA hsa-miR-342-3p CYCS -0.128691966564961 0.561034080130801 0.533584069273294 0.000797541450763797 mirMAP -0.104452481938135 0.00171342515724575 NA NA NA hsa-miR-106a-5p FAS 1.3902608059488 6.07931522284525e-05 -0.878746828084236 0.000340153420442741 miRNAWalker2_validate;miRTarBase -0.274948885695996 7.27787651364869e-15 22431000;27142596 miR 106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS; Bioinformatic analysis combining with validation experiments identified FAS as a direct target of miR-106a; Moreover a significant inverse correlation was found between miR-106a and FAS expression not only in gastric cancer cell lines but also in gastric cancer specimens; Taken together these findings suggest that ectopicly overexpressed miR-106a may play an oncogenic role in gastric carcinogenesis and impair extrinsic apoptotic pathway through targeting FAS;Functional experiment ascertained that miR-106a interacted with FAS and mediated caspase3 pathway tumorigenesis; gastric cancer;gastric cancer hsa-miR-590-5p FAS 2.07441240363458 4.95240551694843e-14 -0.878746828084236 0.000340153420442741 miRanda -0.282578087201106 9.10535063456113e-09 NA NA NA hsa-miR-148b-3p GADD45A 0.483970528590224 0.0026495409465901 0.295807344757299 0.218383996212033 MirTarget -0.340126647931105 6.86475415101894e-07 NA NA NA hsa-miR-203a-3p GADD45A 1.06866854356847 0.00389979689199624 0.295807344757299 0.218383996212033 MirTarget -0.110751485098685 0.000186426373565797 NA NA NA hsa-miR-375 GADD45A 0.615547997686534 0.149199273675144 0.295807344757299 0.218383996212033 miRanda -0.117305912118485 4.96620623755307e-06 NA NA NA hsa-miR-324-3p GADD45B -0.0774700133118458 0.689226297032231 -1.592999067635 1.71286942854757e-12 MirTarget;miRNATAP -0.153520773002313 0.00557801960543285 NA NA NA hsa-miR-590-3p GADD45B 0.83851360565783 0.00129224433453587 -1.592999067635 1.71286942854757e-12 miRanda -0.128384670833959 0.0087523414105501 NA NA NA hsa-miR-127-3p GADD45G -0.723429813065737 0.0192747925933947 0.558251242266569 0.0699587843678354 miRanda;miRNATAP -0.177067134124427 0.000105410370152299 NA NA NA hsa-miR-139-5p GTSE1 -2.26625553116965 7.22375119369519e-16 3.21269345796257 9.56624753375588e-25 miRanda -0.404733295768835 1.81690240635027e-15 NA NA NA hsa-miR-181a-5p GTSE1 -0.375429731222937 0.0562142867129802 3.21269345796257 9.56624753375588e-25 MirTarget -0.415321172338394 6.43934255174038e-08 NA NA NA hsa-miR-181c-5p GTSE1 0.529875814537156 0.0125885822998008 3.21269345796257 9.56624753375588e-25 MirTarget -0.398958505594678 1.78850435853841e-08 NA NA NA hsa-miR-338-3p GTSE1 0.726843067232002 0.0506251324215494 3.21269345796257 9.56624753375588e-25 miRanda -0.214814978835408 1.03042886493979e-07 NA NA NA hsa-miR-455-5p GTSE1 1.36946341828134 5.05118627562807e-08 3.21269345796257 9.56624753375588e-25 miRanda -0.184353334518793 0.00765708362650005 NA NA NA hsa-miR-130b-3p IGF1 1.83046548750752 5.04753914574694e-13 -0.878839289406132 0.00545024687919024 MirTarget -0.208984208427567 0.000411905460472152 NA NA NA hsa-miR-15b-3p IGF1 0.804736041893915 0.000400279611457186 -0.878839289406132 0.00545024687919024 mirMAP -0.294250039955051 1.19648006053704e-05 NA NA NA hsa-miR-16-1-3p IGF1 1.50034693398929 6.63958242991125e-10 -0.878839289406132 0.00545024687919024 mirMAP -0.346697103874593 2.00512825999278e-06 NA NA NA hsa-miR-181b-5p IGF1 0.668793269169928 0.000239998092406404 -0.878839289406132 0.00545024687919024 mirMAP -0.277079678771502 0.000542842928980559 NA NA NA hsa-miR-186-5p IGF1 0.852175445703945 6.88885738584309e-10 -0.878839289406132 0.00545024687919024 mirMAP -0.579822063247168 2.4486690275545e-08 NA NA NA hsa-miR-19a-3p IGF1 2.11776595911517 7.07663078397156e-10 -0.878839289406132 0.00545024687919024 MirTarget -0.295619339516778 1.77416468047122e-09 NA NA NA hsa-miR-19b-1-5p IGF1 1.71217036351784 3.28561659177495e-12 -0.878839289406132 0.00545024687919024 mirMAP -0.353413690663535 1.05429610809513e-07 NA NA NA hsa-miR-19b-3p IGF1 2.10868505662119 5.40846613768389e-17 -0.878839289406132 0.00545024687919024 MirTarget -0.332913498199372 1.17742302735622e-08 NA NA NA hsa-miR-20a-3p IGF1 2.51803599558247 4.92479325298629e-13 -0.878839289406132 0.00545024687919024 mirMAP -0.27191171788815 1.96054552687628e-07 NA NA NA hsa-miR-26b-5p IGF1 0.72362441215247 4.7794659748036e-05 -0.878839289406132 0.00545024687919024 mirMAP -0.268812581966602 0.00103479699566484 NA NA NA hsa-miR-29b-3p IGF1 3.10527711861309 3.01242507282711e-32 -0.878839289406132 0.00545024687919024 MirTarget -0.214642092755044 3.159014552534e-05 25592039 Luciferase reporter assays were conducted to determine the association between miR-29b and the insulin-like growth factor 1 IGF1 3' untranslated region 3'UTR; IGF1 an activator of PI3K/Akt signaling was confirmed as a novel target of miR-29b colorectal cancer hsa-miR-301a-3p IGF1 2.70198002906399 1.56420889710984e-14 -0.878839289406132 0.00545024687919024 MirTarget -0.262923939050952 1.05879075346237e-07 NA NA NA hsa-miR-3065-5p IGF1 0.653308707552535 0.0999497219778851 -0.878839289406132 0.00545024687919024 mirMAP -0.125233094164205 0.00171064732391524 NA NA NA hsa-miR-362-5p IGF1 0.658947634569393 0.0243306847205756 -0.878839289406132 0.00545024687919024 mirMAP -0.262372836237127 2.21880393042342e-07 NA NA NA hsa-miR-450b-5p IGF1 1.69248953402469 1.96052058003353e-07 -0.878839289406132 0.00545024687919024 MirTarget;PITA;mirMAP;miRNATAP -0.155478745947113 0.00168638246971297 NA NA NA hsa-miR-454-3p IGF1 1.49453641052169 4.46235742871434e-09 -0.878839289406132 0.00545024687919024 MirTarget -0.353703568576877 1.23901415685651e-07 NA NA NA hsa-miR-576-5p IGF1 1.02693643167056 1.14846964470173e-06 -0.878839289406132 0.00545024687919024 PITA;mirMAP;miRNATAP -0.319877018116458 2.70279102064343e-06 NA NA NA hsa-miR-590-3p IGF1 0.83851360565783 0.00129224433453587 -0.878839289406132 0.00545024687919024 MirTarget;miRanda;mirMAP;miRNATAP -0.218208367579052 0.00112544995000528 NA NA NA hsa-miR-629-5p IGF1 1.31657090341737 1.45711558652127e-12 -0.878839289406132 0.00545024687919024 mirMAP -0.206791424784461 0.0072984659842286 NA NA NA hsa-miR-3065-5p IGFBP3 0.653308707552535 0.0999497219778851 1.34688121148136 3.19621778611777e-05 MirTarget -0.202153450837598 7.15356608364694e-07 NA NA NA hsa-miR-375 IGFBP3 0.615547997686534 0.149199273675144 1.34688121148136 3.19621778611777e-05 miRNAWalker2_validate -0.106212126293924 0.00251113742917914 NA NA NA hsa-miR-450a-5p MDM2 1.94284269183615 1.52883075909271e-07 -0.018010582169941 0.916081437185101 mirMAP -0.108762560851161 1.20807844291087e-05 NA NA NA hsa-miR-484 MDM2 -0.228576985124486 0.244112867390855 -0.018010582169941 0.916081437185101 miRNAWalker2_validate -0.108672488962364 0.0069392239747807 NA NA NA hsa-let-7a-5p MDM4 -1.37014061604018 3.11451492971224e-14 0.499766401450246 0.00108403645699481 MirTarget;TargetScan -0.117171041955205 0.00217657953672176 NA NA NA hsa-miR-151a-3p MDM4 0.665271883054405 0.000281673348137154 0.499766401450246 0.00108403645699481 mirMAP -0.102080294450266 0.0081710437991246 NA NA NA hsa-miR-152-3p MDM4 0.65734581993875 0.00129552561132481 0.499766401450246 0.00108403645699481 MirTarget -0.249392530208304 1.39849034057286e-13 NA NA NA hsa-miR-222-5p MDM4 0.0957775494210593 0.719329365268725 0.499766401450246 0.00108403645699481 mirMAP -0.186874649332785 5.8314347421584e-13 NA NA NA hsa-miR-361-5p MDM4 0.21454431962475 0.0800957579368424 0.499766401450246 0.00108403645699481 miRanda;mirMAP -0.155647576630015 0.00808853891753239 NA NA NA hsa-miR-146b-5p PERP 1.0922867893808 5.63754607494775e-06 0.779414063228649 0.000233251614906175 miRanda -0.143370125943554 0.000380276603993122 NA NA NA hsa-miR-204-5p PERP -1.01965803752657 0.00767207541525746 0.779414063228649 0.000233251614906175 MirTarget -0.133290795060303 1.84069226491974e-07 NA NA NA hsa-miR-362-3p PERP 0.194097672030785 0.52808475179225 0.779414063228649 0.000233251614906175 miRanda -0.105323836240238 0.00835983684770568 NA NA NA hsa-miR-660-5p PERP 2.04875198906126 1.08269480928897e-17 0.779414063228649 0.000233251614906175 MirTarget -0.117017300139225 0.00395295930976937 NA NA NA hsa-let-7a-5p PMAIP1 -1.37014061604018 3.11451492971224e-14 1.3465665248913 2.20137844697311e-05 TargetScan -0.224781458873572 0.00488496212112512 NA NA NA hsa-miR-200b-3p PMAIP1 1.55157578247391 4.83790020897188e-09 1.3465665248913 2.20137844697311e-05 MirTarget;TargetScan -0.250424090680499 4.05232937169968e-06 NA NA NA hsa-miR-200c-3p PMAIP1 0.380533388300368 0.0842181838538353 1.3465665248913 2.20137844697311e-05 miRNAWalker2_validate;miRTarBase;MirTarget -0.307134734629427 4.00785088372381e-06 NA NA NA hsa-miR-375 PMAIP1 0.615547997686534 0.149199273675144 1.3465665248913 2.20137844697311e-05 miRanda -0.135505400701267 7.92035726580061e-05 NA NA NA hsa-miR-15a-5p PPM1D 1.62575353599172 1.55312121019827e-19 -0.842960797921556 1.98543788687206e-14 MirTarget;miRNATAP -0.200928037013903 1.69503908604022e-13 NA NA NA hsa-miR-26b-5p PPM1D 0.72362441215247 4.7794659748036e-05 -0.842960797921556 1.98543788687206e-14 miRNAWalker2_validate -0.1247090977495 1.78485803573622e-05 NA NA NA hsa-miR-29a-3p PPM1D 0.101650412194573 0.573201097011668 -0.842960797921556 1.98543788687206e-14 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.130558636750052 6.16474771580982e-06 NA NA NA hsa-miR-29a-5p PPM1D 1.89519956688438 1.03453349363915e-11 -0.842960797921556 1.98543788687206e-14 MirTarget -0.190990545441663 9.91833278176573e-20 NA NA NA hsa-miR-29b-3p PPM1D 3.10527711861309 3.01242507282711e-32 -0.842960797921556 1.98543788687206e-14 MirTarget;miRNATAP -0.184744077269729 4.36507328030672e-26 NA NA NA hsa-miR-590-5p PPM1D 2.07441240363458 4.95240551694843e-14 -0.842960797921556 1.98543788687206e-14 miRanda -0.124906422927946 3.08501464952391e-08 NA NA NA hsa-miR-103a-3p PTEN 0.538497806383155 1.88134691850591e-05 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase -0.178416761140974 1.0244122893712e-05 26511107;24828205 LncRNA GAS5 induces PTEN expression through inhibiting miR 103 in endometrial cancer cells; To investigate the expression of GAS5 PTEN and miR-103 RT-PCR was performed; Finally we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells; Through inhibiting the expression of miR-103 GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis and thus could be an important mediator in the pathogenesis of endometrial cancer;Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN ; endometrial cancer;colorectal cancer hsa-miR-106a-5p PTEN 1.3902608059488 6.07931522284525e-05 -0.41901734874011 0.000137135697667474 miRNATAP -0.130885292313782 7.81301360715351e-17 26097565;26318586 miR 106a promotes growth and metastasis of non small cell lung cancer by targeting PTEN; Furthermore the presence of miR-106a was inversely correlated with PTEN in NSCLC tissues; Overall this study suggested that miR-106a inhibited the growth and metastasis of NSCLC cells by decreasing PTEN expression;Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo metastasis; lung squamous cell cancer;prostate cancer hsa-miR-106b-5p PTEN 1.47447672652218 1.32004094930462e-13 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;miRNATAP -0.104117444261304 2.59660457077114e-05 24842611;26238857;26722252 MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance;Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells cell migration;drug resistance; gastric cancer;colorectal cancer;breast cancer hsa-miR-130a-3p PTEN 0.881660100597014 0.000158893668519248 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.101775551899915 2.13139066434031e-06 24490491;27062783;22614869;26837847;27040383;27035216;26043084 Down-regulated miR-130a did not affect cell proliferations but enhanced the sensitivity of the cells to cisplatin inhibited the expressions of MDR1 mRNA and P-gp and increased the expression of PTEN proteins; MiR-130a inhibitor can reverse the cisplatin resistance by upregulating the expression of PTEN proteins and down-regulating P-gp in A2780 cell lines;Platinum-resistant patients had significantly higher levels of expression of miR-130a and BCL-2 and lower level of PTEN than platinum-sensitive patients P < 0.05; MiR-130a may mediate the generation of platinum resistance in epithelial ovarian cancer through inhibiting PTEN to activate PI3K/AKT signaling pathway and increasing BCL-2 to inhibit tumor cell apoptosis;We found that miR-130a was upregulated in SKOV3/CIS compared to the parental SKOV3 cells and PTEN was predicted to be the potential target of miR-130a;The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;In addition by targeting PTEN 3' untranslated region miR-130a might increase cell growth and initiate protein kinase B AKT pathway activation;MicroRNA 130a promotes the metastasis and epithelial mesenchymal transition of osteosarcoma by targeting PTEN; MiR-130a exerted promoting effects on metastatic behavior and EMT of osteosarcoma cells through suppressing PTEN expression;This role of miR-130a may be achieved by regulating the MDR1 and PTEN gene expression drug resistance;drug resistance;;cell migration;;metastasis; ovarian cancer;ovarian cancer;ovarian cancer;bladder cancer;cervical and endocervical cancer;sarcoma;ovarian cancer hsa-miR-130b-3p PTEN 1.83046548750752 5.04753914574694e-13 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.109728666497463 7.00361581570123e-08 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-141-3p PTEN 3.36993755486106 5.42939251147195e-32 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.103983871313954 1.43928589517751e-10 27644195;24742567 Involvement of microRNA 141 3p in 5 fluorouracil and oxaliplatin chemo resistance in esophageal cancer cells via regulation of PTEN; Western blot exhibited altered protein levels of PTEN Akt and PI3k with miR-141-3p inhibitor; An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples; Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo;PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis drug resistance;tumorigenesis esophageal cancer;endometrial cancer hsa-miR-148b-3p PTEN 0.483970528590224 0.0026495409465901 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.130520184239271 3.47762829311644e-05 NA NA NA hsa-miR-16-1-3p PTEN 1.50034693398929 6.63958242991125e-10 -0.41901734874011 0.000137135697667474 MirTarget -0.123272492174072 1.10427479892726e-06 NA NA NA hsa-miR-17-5p PTEN 2.07078488653279 2.77403038572568e-19 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.187397757344705 2.72047461827111e-20 27400681;23391506;23133552;26629823;24462867;26318586;26215320;25634356;26500892;24912422;23418359 GFRα2 prompts cell growth and chemoresistance through down regulating tumor suppressor gene PTEN via Mir 17 5p in pancreatic cancer; Mechanically we discovered that high GFRα2 expression level leads to PTEN inactivation via enhancing Mir-17-5p level;We found that these phenotypes were the results of miR-17 targeting PTEN;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;We hypothesized that knocking down the oncogenic microRNA oncomiR miR-17-5p might restore the expression levels of PDCD4 and PTEN tumor suppressor proteins illustrating a route to oligonucleotide therapy of TNBC; Contrary to conventional wisdom antisense knockdown of oncomiR miR-17-5p guide strand reduced PDCD4 and PTEN proteins by 1.8±0.3 fold in human TNBC cells instead of raising them; Bioinformatics analysis and folding energy calculations revealed that mRNA targets of miR-17-5p guide strand such as PDCD4 and PTEN could also be regulated by miR-17-3p passenger strand;miR 17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression; Expression of miR-17 was negatively correlated with PTEN in OS tissues;Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR 17 family in prostate cancer; Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo;In addition ERβ expression significantly increased in calycosin-treated HCT-116 cells followed by a decrease of miR-17 and up-regulation of PTEN; Our results indicate that calycosin has an inhibitory effect on CRC which might be obtained by ERβ-mediated regulation of miR-17 and PTEN expression;The High Expression of the microRNA 17 92 Cluster and its Paralogs and the Downregulation of the Target Gene PTEN Is Associated with Primary Cutaneous B Cell Lymphoma Progression;MicroRNA 17 5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling; miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells;MicroRNA 17 5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression; We found that PTEN was a target of miR-17-5p in the colon cancer cells and their context-specific interactions were responsible for multiple drug-resistance; Chemotherapy was found to increase the expression levels of miR-17-5p which further repressed PTEN levels contributing to the development of chemo-resistance; MiR-17-5p is a predictive factor for chemotherapy response and a prognostic factor for overall survival in CRC which is due to its regulation of PTEN expression;The mature miR-17-5p exerted this function by repressing the expression of PTEN drug resistance;;;;metastasis;;;progression;drug resistance;metastasis;drug resistance;poor survival; pancreatic cancer;glioblastoma;sarcoma;breast cancer;sarcoma;prostate cancer;colorectal cancer;B cell lymphoma;ovarian cancer;colorectal cancer;liver cancer hsa-miR-186-5p PTEN 0.852175445703945 6.88885738584309e-10 -0.41901734874011 0.000137135697667474 mirMAP;miRNATAP -0.18571264902072 2.84306242795631e-07 NA NA NA hsa-miR-18a-5p PTEN 1.37435209536521 9.2173853686829e-06 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase -0.141101959733646 1.18775251717657e-16 24681249;27291152 However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis;miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis metastasis; colon cancer;esophageal cancer hsa-miR-193a-3p PTEN 0.549173467360291 0.0319030569365466 -0.41901734874011 0.000137135697667474 PITA;miRanda -0.100107289825292 2.74866825496953e-06 26753960;23223432 Downregulation of microRNA 193 3p inhibits tumor proliferation migration and chemoresistance in human gastric cancer by regulating PTEN gene;Our study identifies miR-193a and PTEN as targets for AML1/ETO and provides evidence that links the epigenetic silencing of tumor suppressor genes miR-193a and PTEN to differentiation block of myeloid precursors drug resistance;differentiation gastric cancer;acute myeloid leukemia hsa-miR-19a-3p PTEN 2.11776595911517 7.07663078397156e-10 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.152449210349358 4.83140828337759e-20 26098000;27289489;27445062;24831732;21853360;24681249;25107371 Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;Transfection of miR-19a and -19b mimics reversed the up-regulations of IGF2R and PTEN gene expression and abrogated the GSE induced anti-proliferative response;The target genes of miR-19a such as ABCA1 and PTEN that had been suppressed by miR recovered their expression through CAP treatment;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN;The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid; Meanwhile gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN; The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN ;drug resistance;;;drug resistance;metastasis; lung cancer;lung cancer;breast cancer;breast cancer;breast cancer;colon cancer;bladder cancer hsa-miR-19b-3p PTEN 2.10868505662119 5.40846613768389e-17 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.144424393781319 5.95919159731141e-13 26098000;24831732;21853360;24681249 Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN ;;drug resistance;metastasis lung cancer;breast cancer;breast cancer;colon cancer hsa-miR-20a-5p PTEN 2.64649625616975 5.1469418982085e-27 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;miRNATAP -0.161121765339806 1.05381764637456e-17 26031366 The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction; MiR-20a levels were increased in HCC cell lines and tissues whereas PTEN was inversely correlated with it; PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance drug resistance liver cancer hsa-miR-25-3p PTEN 0.361927330834176 0.0163670566170988 -0.41901734874011 0.000137135697667474 miRTarBase;MirTarget;miRNATAP -0.11968562938759 0.000411956693911945 NA NA NA hsa-miR-26b-5p PTEN 0.72362441215247 4.7794659748036e-05 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.101786450251327 0.000339227897329926 26068649 Down regulation of microRNA 26b modulates non small cell lung cancer cells chemoresistance and migration through the association of PTEN; It indicates that miR-26b may regulate NSCLC migration and chemosensitivity through the regulation of PTEN drug resistance lung squamous cell cancer hsa-miR-301a-3p PTEN 2.70198002906399 1.56420889710984e-14 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.136185588556865 5.91738747356386e-16 24315818;26846737 Upregulated microRNA 301a in breast cancer promotes tumor metastasis by targeting PTEN and activating Wnt/β catenin signaling; Furthermore miR-301a directly targeted and suppressed PTEN one negative regulator of the Wnt/β-catenin signaling cascade; These results demonstrate that miR-301a maintains constitutively activated Wnt/β-catenin signaling by directly targeting PTEN which promotes breast cancer invasion and metastasis;MicroRNA 301a promotes cell proliferation via PTEN targeting in Ewing's sarcoma cells; Our results demonstrated the novel mechanism controlling PTEN expression via miR-301a in ES cells metastasis; breast cancer;sarcoma hsa-miR-342-3p PTEN -0.128691966564961 0.561034080130801 -0.41901734874011 0.000137135697667474 miRanda -0.12660675779428 2.51472806501277e-08 NA NA NA hsa-miR-425-5p PTEN 1.21659524959201 8.01126418494715e-09 -0.41901734874011 0.000137135697667474 miRNATAP -0.169099257663055 2.9682618955533e-13 25154996 An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation gastric cancer hsa-miR-454-3p PTEN 1.49453641052169 4.46235742871434e-09 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.207669245917415 3.06891122776166e-20 26296312;27261580 MicroRNA 454 functions as an oncogene by regulating PTEN in uveal melanoma; Furthermore we identified PTEN as a direct target of miR-454; Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells;MiR 454 promotes the progression of human non small cell lung cancer and directly targets PTEN; At last the potential regulatory function of miR-454 on PTEN expression was confirmed; Further PTEN was confirmed as a direct target of miR-454 by using Luciferase Reporter Assay ;progression melanoma;lung squamous cell cancer hsa-miR-590-5p PTEN 2.07441240363458 4.95240551694843e-14 -0.41901734874011 0.000137135697667474 mirMAP -0.163531572056391 4.57428223021788e-14 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-7-1-3p PTEN 2.61364240402652 1.98959237815786e-32 -0.41901734874011 0.000137135697667474 mirMAP -0.12353194205425 3.34150554364966e-08 NA NA NA hsa-miR-92a-3p PTEN -0.136362762339122 0.493412263619364 -0.41901734874011 0.000137135697667474 MirTarget;miRNATAP -0.123586887613975 1.14670739856895e-06 26432332;25515201;24137349;23546593;23133552;24026406 Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor; Taken together these findings suggested that miR-92a could promote growth metastasis and chemoresistance in NSCLC cells at least partially by targeting PTEN;MiR 92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN Mediated PI3K/AKT Pathway; The expression of miR-92a PTEN and E-cadherin was analyzed by real-time PCR; In addition there was a negative correlation between levels of miR-92a and the PTEN gene p < 0.0001; The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;The expression levels of miR-92a and phosphatase and tensin homologue PTEN were detected by qRT-PCR and western blot; In addition the regulation of PTEN by miR-92a was evaluated by qRT-PCR western blot and luciferase reporter assays; There was an inverse correlation between the levels of miR-92a and PTEN in CRC tissues; The overexpression of miR-92a in CRC cells decreased PTEN expression at the translational level and decreased PTEN-driven luciferase-reporter activity; Our results demonstrated that miR-92a induced EMT and regulated cell growth migration and invasion in the SW480 cells at least partially via suppression of PTEN expression metastasis;drug resistance;metastasis;cell migration;;worse prognosis;; lung squamous cell cancer;colorectal cancer;cervical and endocervical cancer;liver cancer;sarcoma;colorectal cancer hsa-miR-93-5p PTEN 1.50844106515467 1.83836866996875e-12 -0.41901734874011 0.000137135697667474 miRNAWalker2_validate;miRTarBase;miRNATAP -0.209970443777429 3.2573256879644e-21 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-let-7a-5p RRM2 -1.37014061604018 3.11451492971224e-14 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate;TargetScan;miRNATAP -0.577637163480958 1.21792374300508e-12 NA NA NA hsa-let-7b-5p RRM2 -1.62016148171663 1.56547487335694e-15 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate;miRNATAP -0.642149930538833 9.54316290267664e-20 NA NA NA hsa-let-7d-5p RRM2 -0.618145137285851 6.06067642715858e-05 2.86899871288903 2.94977476028636e-19 miRNATAP -0.308003086167344 0.00204221442204009 NA NA NA hsa-let-7e-5p RRM2 -0.749418249305995 7.34668338492227e-05 2.86899871288903 2.94977476028636e-19 miRNATAP -0.220334141134382 0.00661734239433178 NA NA NA hsa-let-7g-5p RRM2 0.0837630831079359 0.583083118232597 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate;miRNATAP -0.313003054815174 0.0021017280758929 NA NA NA hsa-miR-100-5p RRM2 0.423440403609931 0.104072937074013 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate -0.163769019663704 0.00550492160889045 NA NA NA hsa-miR-125a-5p RRM2 -1.04824536696301 3.97386082850476e-07 2.86899871288903 2.94977476028636e-19 miRanda -0.674362297113934 1.0793634299499e-21 NA NA NA hsa-miR-199b-5p RRM2 2.13822205171062 4.56262988762436e-15 2.86899871288903 2.94977476028636e-19 miRanda -0.143041636025161 0.00882214834330692 NA NA NA hsa-miR-26a-5p RRM2 -0.126411409001078 0.440028840408711 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate -0.684477705219282 1.29123506933786e-13 NA NA NA hsa-miR-30a-5p RRM2 -0.924084264430689 0.000762030454634728 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate -0.558413286339026 3.31498901558465e-26 NA NA NA hsa-miR-30c-5p RRM2 -0.327319105755825 0.123595832152687 2.86899871288903 2.94977476028636e-19 miRNAWalker2_validate -0.641697913237742 3.02073515186064e-20 NA NA NA hsa-miR-362-3p RRM2 0.194097672030785 0.52808475179225 2.86899871288903 2.94977476028636e-19 miRanda -0.169111644050997 0.00681807082710627 NA NA NA hsa-miR-142-3p RRM2B 3.98476449801341 1.56256182021111e-35 -0.564869102427815 0.000329349327584025 miRNAWalker2_validate;miRanda -0.120927069390427 4.99065993047701e-09 NA NA NA hsa-miR-186-5p RRM2B 0.852175445703945 6.88885738584309e-10 -0.564869102427815 0.000329349327584025 mirMAP -0.242866564685438 3.01982245563765e-06 NA NA NA hsa-miR-324-5p RRM2B 1.06955541886548 5.39722006604758e-05 -0.564869102427815 0.000329349327584025 miRanda -0.152238887924028 1.58044786455217e-08 NA NA NA hsa-miR-590-3p RRM2B 0.83851360565783 0.00129224433453587 -0.564869102427815 0.000329349327584025 miRanda -0.181957411083383 3.51095155772326e-08 NA NA NA hsa-miR-590-5p RRM2B 2.07441240363458 4.95240551694843e-14 -0.564869102427815 0.000329349327584025 miRanda;mirMAP -0.27932442631006 8.49259712160949e-20 NA NA NA hsa-miR-7-1-3p RRM2B 2.61364240402652 1.98959237815786e-32 -0.564869102427815 0.000329349327584025 mirMAP -0.206892121918884 8.67282653489326e-11 NA NA NA hsa-miR-30b-5p SERPINE1 0.358144314839612 0.138026229059594 -0.114178653022812 0.73682517077286 MirTarget -0.358064427205798 2.30487975154751e-08 25170877 miR-30b may function as a novel tumor suppressor gene in gastric cancer by targeting PAI-1 and regulating the apoptosis of cancer cells gastric cancer hsa-miR-30c-5p SERPINE1 -0.327319105755825 0.123595832152687 -0.114178653022812 0.73682517077286 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.284046002981584 0.00011264609085013 NA NA NA hsa-miR-30d-5p SERPINE1 -0.920901858717441 3.50898334321668e-05 -0.114178653022812 0.73682517077286 miRNATAP -0.387251756384648 2.03451245658885e-08 NA NA NA hsa-miR-15a-5p SESN1 1.62575353599172 1.55312121019827e-19 -1.80580674870285 1.4714514385367e-22 MirTarget;miRNATAP -0.154734566762702 0.00122611667103779 NA NA NA hsa-miR-183-5p SESN1 2.39247234736657 3.92444525331999e-21 -1.80580674870285 1.4714514385367e-22 MirTarget -0.21955896396407 3.05635239623436e-11 NA NA NA hsa-miR-21-5p SESN1 4.38165933891779 1.52536633902505e-92 -1.80580674870285 1.4714514385367e-22 miRNAWalker2_validate -0.347624018205389 2.35349864344463e-27 NA NA NA hsa-miR-22-3p SESN1 1.42501909916631 1.72295005910055e-25 -1.80580674870285 1.4714514385367e-22 MirTarget -0.224479844524303 0.000329767147593314 NA NA NA hsa-miR-28-5p SESN1 1.19639196514745 4.59365801666536e-18 -1.80580674870285 1.4714514385367e-22 PITA;miRanda;miRNATAP -0.207225809746842 0.00100137593234134 NA NA NA hsa-miR-424-5p SESN1 1.26485330287919 6.1498991028257e-06 -1.80580674870285 1.4714514385367e-22 MirTarget;miRNATAP -0.121342221085396 0.000117496170259704 NA NA NA hsa-miR-590-3p SESN1 0.83851360565783 0.00129224433453587 -1.80580674870285 1.4714514385367e-22 miRanda -0.152868009746262 0.000183621179094314 NA NA NA hsa-miR-590-5p SESN1 2.07441240363458 4.95240551694843e-14 -1.80580674870285 1.4714514385367e-22 miRanda -0.244563219659774 2.13328813903746e-10 NA NA NA hsa-miR-106a-5p SESN3 1.3902608059488 6.07931522284525e-05 -0.0968711060818062 0.738378790058081 MirTarget -0.245915762255091 5.19536188143987e-09 NA NA NA hsa-miR-106b-5p SESN3 1.47447672652218 1.32004094930462e-13 -0.0968711060818062 0.738378790058081 MirTarget -0.303554221942107 3.00618709751669e-06 NA NA NA hsa-miR-130b-3p SESN3 1.83046548750752 5.04753914574694e-13 -0.0968711060818062 0.738378790058081 mirMAP -0.176189771923799 0.0011245229850793 NA NA NA hsa-miR-142-3p SESN3 3.98476449801341 1.56256182021111e-35 -0.0968711060818062 0.738378790058081 miRanda -0.115547726370626 0.00265743983704141 NA NA NA hsa-miR-16-2-3p SESN3 0.503964803530872 0.0263648405197492 -0.0968711060818062 0.738378790058081 mirMAP -0.254241706904614 6.52583681399384e-05 NA NA NA hsa-miR-17-5p SESN3 2.07078488653279 2.77403038572568e-19 -0.0968711060818062 0.738378790058081 MirTarget;TargetScan -0.320514863614511 5.21782578112463e-09 NA NA NA hsa-miR-186-5p SESN3 0.852175445703945 6.88885738584309e-10 -0.0968711060818062 0.738378790058081 MirTarget -0.298335480830019 0.001895801510945 NA NA NA hsa-miR-19b-1-5p SESN3 1.71217036351784 3.28561659177495e-12 -0.0968711060818062 0.738378790058081 MirTarget -0.247712123388203 5.01611763035567e-05 NA NA NA hsa-miR-20a-3p SESN3 2.51803599558247 4.92479325298629e-13 -0.0968711060818062 0.738378790058081 mirMAP -0.190147613621213 7.5055740057638e-05 NA NA NA hsa-miR-20a-5p SESN3 2.64649625616975 5.1469418982085e-27 -0.0968711060818062 0.738378790058081 MirTarget -0.263385739109084 2.05937890311342e-07 NA NA NA hsa-miR-20b-5p SESN3 1.35770573270947 0.00260808530013016 -0.0968711060818062 0.738378790058081 MirTarget -0.162718772866176 1.17014214229879e-07 NA NA NA hsa-miR-25-3p SESN3 0.361927330834176 0.0163670566170988 -0.0968711060818062 0.738378790058081 MirTarget;miRNATAP -0.262570279749937 0.00329514714870453 NA NA NA hsa-miR-29a-5p SESN3 1.89519956688438 1.03453349363915e-11 -0.0968711060818062 0.738378790058081 MirTarget;mirMAP -0.218202869457075 9.25535338942161e-05 NA NA NA hsa-miR-29b-1-5p SESN3 1.71024742617744 3.64915691793611e-09 -0.0968711060818062 0.738378790058081 mirMAP -0.167291449721191 0.000208415262355897 NA NA NA hsa-miR-301a-3p SESN3 2.70198002906399 1.56420889710984e-14 -0.0968711060818062 0.738378790058081 mirMAP -0.195699293103833 1.63047518859318e-05 NA NA NA hsa-miR-320b SESN3 0.226640952564488 0.378818692487242 -0.0968711060818062 0.738378790058081 miRanda;mirMAP -0.166865438710542 0.00129195180283279 NA NA NA hsa-miR-339-5p SESN3 0.536997313601114 0.0488090394176611 -0.0968711060818062 0.738378790058081 miRanda -0.168553519287294 0.000559477473206441 NA NA NA hsa-miR-33a-5p SESN3 2.80127809675219 5.09053890529424e-12 -0.0968711060818062 0.738378790058081 mirMAP -0.138483941384921 0.000483597007254487 NA NA NA hsa-miR-363-3p SESN3 -0.301366430789287 0.414451883803084 -0.0968711060818062 0.738378790058081 MirTarget -0.159654537664969 9.25473971779135e-06 NA NA NA hsa-miR-454-3p SESN3 1.49453641052169 4.46235742871434e-09 -0.0968711060818062 0.738378790058081 mirMAP -0.337138840120706 3.2800111541038e-08 NA NA NA hsa-miR-500a-5p SESN3 0.648155655714409 0.0104657432932038 -0.0968711060818062 0.738378790058081 mirMAP -0.241762596615644 6.8679877392691e-06 NA NA NA hsa-miR-576-5p SESN3 1.02693643167056 1.14846964470173e-06 -0.0968711060818062 0.738378790058081 mirMAP -0.310750485424549 5.7704401487526e-07 NA NA NA hsa-miR-582-5p SESN3 1.08286181406653 0.00149040450161757 -0.0968711060818062 0.738378790058081 MirTarget -0.147821717495032 0.000206604463129331 NA NA NA hsa-miR-589-3p SESN3 1.34253876396688 1.84935927493927e-05 -0.0968711060818062 0.738378790058081 MirTarget;mirMAP -0.113732821954677 0.00909065981232026 NA NA NA hsa-miR-590-3p SESN3 0.83851360565783 0.00129224433453587 -0.0968711060818062 0.738378790058081 miRNAWalker2_validate;MirTarget;miRanda -0.275497427158523 6.00422405103357e-06 NA NA NA hsa-miR-7-1-3p SESN3 2.61364240402652 1.98959237815786e-32 -0.0968711060818062 0.738378790058081 MirTarget;mirMAP -0.19411315784066 0.00110224932445762 NA NA NA hsa-miR-92a-3p SESN3 -0.136362762339122 0.493412263619364 -0.0968711060818062 0.738378790058081 MirTarget;miRNATAP -0.262781286032761 9.10655775232688e-05 NA NA NA hsa-miR-93-5p SESN3 1.50844106515467 1.83836866996875e-12 -0.0968711060818062 0.738378790058081 MirTarget -0.35497376188872 3.57902434434351e-09 NA NA NA hsa-miR-96-5p SESN3 3.0381097138467 4.40362891630201e-24 -0.0968711060818062 0.738378790058081 MirTarget;TargetScan -0.18573570856152 0.000131001454194109 NA NA NA hsa-miR-128-3p STEAP3 1.03509868349735 2.11606310588315e-10 0.548156881605402 0.0179406069585327 MirTarget -0.317580783613681 7.74543021234429e-07 NA NA NA hsa-miR-324-3p STEAP3 -0.0774700133118458 0.689226297032231 0.548156881605402 0.0179406069585327 MirTarget -0.163530795697962 0.00320402805025689 NA NA NA hsa-miR-326 STEAP3 -0.992397386750901 0.00335173765975096 0.548156881605402 0.0179406069585327 miRanda -0.112015832687058 0.000354092707185726 NA NA NA hsa-miR-330-5p STEAP3 0.168520831101569 0.336433937906706 0.548156881605402 0.0179406069585327 miRanda -0.188058481236838 0.00218822893303162 NA NA NA hsa-miR-484 STEAP3 -0.228576985124486 0.244112867390855 0.548156881605402 0.0179406069585327 MirTarget -0.14550652552539 0.00788923312412242 NA NA NA hsa-miR-576-5p STEAP3 1.02693643167056 1.14846964470173e-06 0.548156881605402 0.0179406069585327 mirMAP -0.180591832975063 0.0003111184119428 NA NA NA hsa-miR-92a-3p STEAP3 -0.136362762339122 0.493412263619364 0.548156881605402 0.0179406069585327 miRNAWalker2_validate -0.174664244709528 0.00119156840090644 NA NA NA hsa-miR-16-1-3p THBS1 1.50034693398929 6.63958242991125e-10 -0.930928104430071 0.00139531304277658 MirTarget -0.335212349500693 6.0842815757812e-07 NA NA NA hsa-miR-17-5p THBS1 2.07078488653279 2.77403038572568e-19 -0.930928104430071 0.00139531304277658 miRNAWalker2_validate -0.438129032548489 9.5201094057219e-16 NA NA NA hsa-miR-182-5p THBS1 3.21839823004635 5.13049145201115e-39 -0.930928104430071 0.00139531304277658 MirTarget -0.17203897957724 0.000598074099312019 24053448 Effects of anti miR 182 on TSP 1 expression in human colon cancer cells: there is a sense in antisense?; We investigate on the molecular mechanism by which miR-182 could regulate thrombospondin-1 TSP-1 expression a protein downregulated in CRC and inversely correlated with tumor vascularity and metastasis; miR-182 over-expressed in colorectal cancer CRC has like predictive target thrombospondin-1 TSP-1 a protein inversely correlated with tumor vascularity and metastasis that results downregulated in different types of cancer including CRC; We found that TSP-1 increased after transfection with anti-miR-182 and we showed that miR-182 targets TSP-1 3'UTR-mRNA in both cells; Our data suggest that miR-182 targets the anti-angiogenic factor TSP-1 and that anti-miR-182 determines an upregulation of TSP-1 expression in colon cancer cells metastasis colon cancer hsa-miR-186-5p THBS1 0.852175445703945 6.88885738584309e-10 -0.930928104430071 0.00139531304277658 mirMAP -0.519985070059 6.04454698272776e-08 NA NA NA hsa-miR-18a-5p THBS1 1.37435209536521 9.2173853686829e-06 -0.930928104430071 0.00139531304277658 MirTarget -0.297317863017191 9.33481417958521e-11 NA NA NA hsa-miR-19a-3p THBS1 2.11776595911517 7.07663078397156e-10 -0.930928104430071 0.00139531304277658 MirTarget;mirMAP -0.320107454551527 1.0910865655915e-12 NA NA NA hsa-miR-19b-3p THBS1 2.10868505662119 5.40846613768389e-17 -0.930928104430071 0.00139531304277658 MirTarget;mirMAP -0.394071669543936 1.30352702952378e-13 NA NA NA hsa-miR-200a-5p THBS1 2.26680634475411 1.52168554660094e-17 -0.930928104430071 0.00139531304277658 MirTarget -0.192969334171335 7.36724513214628e-05 NA NA NA hsa-miR-200b-5p THBS1 0.5652412449293 0.0273948420835934 -0.930928104430071 0.00139531304277658 MirTarget -0.190414406051537 0.000286594153768624 NA NA NA hsa-miR-20a-5p THBS1 2.64649625616975 5.1469418982085e-27 -0.930928104430071 0.00139531304277658 miRNAWalker2_validate -0.389697563087682 9.68073828210161e-15 NA NA NA hsa-miR-29b-1-5p THBS1 1.71024742617744 3.64915691793611e-09 -0.930928104430071 0.00139531304277658 mirMAP -0.174722185271877 0.000124277285903 NA NA NA hsa-miR-3065-5p THBS1 0.653308707552535 0.0999497219778851 -0.930928104430071 0.00139531304277658 MirTarget -0.146963541534871 6.23555676304228e-05 NA NA NA hsa-miR-32-5p THBS1 0.876853679269853 6.04859399018691e-05 -0.930928104430071 0.00139531304277658 mirMAP -0.378697285956757 8.57166070787422e-09 NA NA NA hsa-miR-335-3p THBS1 1.51116383169018 6.88553098788624e-13 -0.930928104430071 0.00139531304277658 mirMAP -0.277769513126927 8.06336576747616e-06 NA NA NA hsa-miR-338-3p THBS1 0.726843067232002 0.0506251324215494 -0.930928104430071 0.00139531304277658 MirTarget;PITA;miRanda -0.137206796252208 0.000139893109055389 NA NA NA hsa-miR-500a-5p THBS1 0.648155655714409 0.0104657432932038 -0.930928104430071 0.00139531304277658 mirMAP -0.266623772340649 8.55185956464653e-07 NA NA NA hsa-miR-576-5p THBS1 1.02693643167056 1.14846964470173e-06 -0.930928104430071 0.00139531304277658 mirMAP -0.283940645214832 6.42767093777526e-06 NA NA NA hsa-miR-590-3p THBS1 0.83851360565783 0.00129224433453587 -0.930928104430071 0.00139531304277658 miRanda -0.190498848920156 0.00206214930389577 NA NA NA hsa-miR-590-5p THBS1 2.07441240363458 4.95240551694843e-14 -0.930928104430071 0.00139531304277658 miRanda;mirMAP -0.246253114774633 2.83321183197093e-05 NA NA NA hsa-miR-664a-3p THBS1 0.443077463053857 0.0214248925850984 -0.930928104430071 0.00139531304277658 mirMAP -0.228448076947888 0.00112527165905494 NA NA NA hsa-miR-671-5p THBS1 2.24156654191798 2.10433241567652e-14 -0.930928104430071 0.00139531304277658 MirTarget -0.190134986140565 0.000356438440186681 NA NA NA hsa-miR-7-1-3p THBS1 2.61364240402652 1.98959237815786e-32 -0.930928104430071 0.00139531304277658 MirTarget;mirMAP -0.299067024820193 5.25045592977195e-07 NA NA NA hsa-miR-92a-3p THBS1 -0.136362762339122 0.493412263619364 -0.930928104430071 0.00139531304277658 miRNAWalker2_validate -0.339440045365484 4.84769739541811e-07 NA NA NA hsa-miR-940 TNFRSF10B 0.011138136211269 0.974925352379525 0.178447201136893 0.311593856462823 miRNAWalker2_validate -0.141318772516878 2.62411186947218e-08 NA NA NA hsa-miR-335-5p TP73 -0.466533891325811 0.0676987038122831 0.747565174453192 0.029826592573533 mirMAP -0.236913481774427 0.000654611579572471 NA NA NA hsa-miR-106a-5p ZMAT3 1.3902608059488 6.07931522284525e-05 -0.318761613695159 0.0781412592604581 mirMAP -0.174605043556167 2.3623006283548e-11 NA NA NA hsa-miR-106b-5p ZMAT3 1.47447672652218 1.32004094930462e-13 -0.318761613695159 0.0781412592604581 mirMAP -0.139782253545925 0.000609351557127886 NA NA NA hsa-miR-130a-3p ZMAT3 0.881660100597014 0.000158893668519248 -0.318761613695159 0.0781412592604581 MirTarget -0.159248989625711 6.44576990463673e-06 NA NA NA hsa-miR-130b-3p ZMAT3 1.83046548750752 5.04753914574694e-13 -0.318761613695159 0.0781412592604581 MirTarget -0.145816368009431 1.43589783130008e-05 NA NA NA hsa-miR-148b-5p ZMAT3 1.39335695292409 2.13220380550073e-07 -0.318761613695159 0.0781412592604581 MirTarget -0.200039552590801 3.76729688075804e-08 NA NA NA hsa-miR-15a-5p ZMAT3 1.62575353599172 1.55312121019827e-19 -0.318761613695159 0.0781412592604581 MirTarget -0.315764667108568 4.79264002102154e-13 NA NA NA hsa-miR-17-5p ZMAT3 2.07078488653279 2.77403038572568e-19 -0.318761613695159 0.0781412592604581 mirMAP -0.252267665468309 9.46029909724436e-14 NA NA NA hsa-miR-186-5p ZMAT3 0.852175445703945 6.88885738584309e-10 -0.318761613695159 0.0781412592604581 MirTarget -0.356866871140414 1.55507848094842e-09 NA NA NA hsa-miR-19a-3p ZMAT3 2.11776595911517 7.07663078397156e-10 -0.318761613695159 0.0781412592604581 MirTarget -0.278372428105584 6.30054019410652e-25 NA NA NA hsa-miR-19b-3p ZMAT3 2.10868505662119 5.40846613768389e-17 -0.318761613695159 0.0781412592604581 MirTarget -0.252990625834856 1.24350607124492e-14 NA NA NA hsa-miR-20a-5p ZMAT3 2.64649625616975 5.1469418982085e-27 -0.318761613695159 0.0781412592604581 mirMAP -0.24637267458442 2.13310256745677e-15 NA NA NA hsa-miR-22-5p ZMAT3 1.71436244278242 9.85038001236064e-15 -0.318761613695159 0.0781412592604581 mirMAP -0.180586173203754 6.35998466897797e-07 NA NA NA hsa-miR-29b-1-5p ZMAT3 1.71024742617744 3.64915691793611e-09 -0.318761613695159 0.0781412592604581 mirMAP -0.138125079373352 8.06410898406188e-07 NA NA NA hsa-miR-301a-3p ZMAT3 2.70198002906399 1.56420889710984e-14 -0.318761613695159 0.0781412592604581 MirTarget -0.200071825663159 6.72258346497952e-13 NA NA NA hsa-miR-362-3p ZMAT3 0.194097672030785 0.52808475179225 -0.318761613695159 0.0781412592604581 MirTarget -0.170336147911008 3.9121140819292e-07 NA NA NA hsa-miR-362-5p ZMAT3 0.658947634569393 0.0243306847205756 -0.318761613695159 0.0781412592604581 mirMAP -0.247610949337513 1.27575468631098e-18 NA NA NA hsa-miR-374a-5p ZMAT3 -0.197027254874826 0.298080902192861 -0.318761613695159 0.0781412592604581 mirMAP -0.132007326638438 0.00783443978297406 NA NA NA hsa-miR-425-5p ZMAT3 1.21659524959201 8.01126418494715e-09 -0.318761613695159 0.0781412592604581 MirTarget -0.239826361973952 4.01546940307034e-10 NA NA NA hsa-miR-429 ZMAT3 2.3794516574089 1.72813419703734e-13 -0.318761613695159 0.0781412592604581 MirTarget;miRNATAP -0.104551391799815 0.000114833933352168 NA NA NA hsa-miR-454-3p ZMAT3 1.49453641052169 4.46235742871434e-09 -0.318761613695159 0.0781412592604581 MirTarget -0.343705078787277 1.4782916231988e-20 NA NA NA hsa-miR-501-5p ZMAT3 0.411357595674671 0.10435288464149 -0.318761613695159 0.0781412592604581 MirTarget;mirMAP -0.223999145487047 3.62840278253431e-12 NA NA NA hsa-miR-576-5p ZMAT3 1.02693643167056 1.14846964470173e-06 -0.318761613695159 0.0781412592604581 MirTarget -0.258072004838115 1.78034764065371e-11 NA NA NA hsa-miR-590-3p ZMAT3 0.83851360565783 0.00129224433453587 -0.318761613695159 0.0781412592604581 mirMAP;miRNATAP -0.143008576248367 0.000175656490032134 NA NA NA hsa-miR-590-5p ZMAT3 2.07441240363458 4.95240551694843e-14 -0.318761613695159 0.0781412592604581 miRanda -0.209279883045496 6.38087986161362e-09 NA NA NA hsa-miR-660-5p ZMAT3 2.04875198906126 1.08269480928897e-17 -0.318761613695159 0.0781412592604581 mirMAP -0.184750989084003 6.00255292295678e-08 NA NA NA hsa-miR-664a-3p ZMAT3 0.443077463053857 0.0214248925850984 -0.318761613695159 0.0781412592604581 mirMAP -0.225835191255474 1.46158460999625e-07 NA NA NA hsa-miR-671-5p ZMAT3 2.24156654191798 2.10433241567652e-14 -0.318761613695159 0.0781412592604581 MirTarget -0.233631969162751 4.17203506189094e-13 NA NA NA hsa-miR-93-5p ZMAT3 1.50844106515467 1.83836866996875e-12 -0.318761613695159 0.0781412592604581 mirMAP -0.283706913468208 1.95195563912598e-14 NA NA NA