miRNA	gene	miRNA_log2FC	miRNA_pvalue	gene_log2FC	gene_pvalue	interactions	correlation_beta	correlation_pvalue	PMID	evidence	outcome	cancer
hsa-miR-21-5p	ABCA1	-0.142759573450327	0.928848736959803	-0.0995439270907923	0.842197222283446	mirMAP	-0.315513734860042	2.292461338917e-19		NA	NA	NA
hsa-miR-21-5p	ABCA10	-0.142759573450327	0.928848736959803	-0.772650628980005	0.00842284646112155	mirMAP	-1.05427906803444	2.94764840103498e-42		NA	NA	NA
hsa-miR-21-5p	ACAT1	-0.142759573450327	0.928848736959803	-0.225546197697149	0.678037623208382	miRNAWalker2_validate	-0.298691444140465	4.98274156830538e-28		NA	NA	NA
hsa-miR-21-5p	ACER3	-0.142759573450327	0.928848736959803	0.141219347192632	0.714359302417719	mirMAP	-0.269892772906774	3.09742643677186e-15		NA	NA	NA
hsa-miR-21-5p	ACVR2A	-0.142759573450327	0.928848736959803	0.0815198193273972	0.830572393580105	miRNATAP	-0.228019435065362	1.72805692634805e-21		NA	NA	NA
hsa-miR-21-5p	AKAP6	-0.142759573450327	0.928848736959803	0.345580394451491	0.127865388572229	MirTarget	-0.608209651033571	2.41703851935952e-42		NA	NA	NA
hsa-miR-21-5p	AKAP9	-0.142759573450327	0.928848736959803	-0.462267439312979	0.455329433420925	miRNAWalker2_validate	-0.168978346657109	4.29479028294113e-08		NA	NA	NA
hsa-miR-21-5p	AKIRIN1	-0.142759573450327	0.928848736959803	0.446433031055865	0.4081982619252	miRNATAP	-0.105340715301105	8.03937220548246e-09		NA	NA	NA
hsa-miR-21-5p	ANKRD46	-0.142759573450327	0.928848736959803	-0.1344170123369	0.748646537924079	miRNAWalker2_validate;miRTarBase	-0.309003306442088	3.15456465435422e-33	21219636	Knockdown of miR-21 significantly increased the expression of ANKRD46 at both mRNA and protein levels; ANKRD46 is newly identified as a direct target of miR-21 in BC		breast cancer
hsa-miR-21-5p	ANKS1B	-0.142759573450327	0.928848736959803	-1.35978918186109	3.06791627628134e-08	MirTarget	-0.121716593374487	0.0142128877072745		NA	NA	NA
hsa-miR-21-5p	ANO3	-0.142759573450327	0.928848736959803	0.123063680348756	0.760800337215545	mirMAP	-0.867081885789892	1.73647926570769e-18		NA	NA	NA
hsa-miR-21-5p	ANXA1	-0.142759573450327	0.928848736959803	1.16235935076641	0.0957888206317983	MirTarget	-0.469016266891393	7.01388858801368e-26		NA	NA	NA
hsa-miR-21-5p	APC	-0.142759573450327	0.928848736959803	-0.543855103612224	0.253015890893651	miRNAWalker2_validate	-0.246283784759972	7.23712061544539e-21	23773491;24832083	The prognostic significance of APC gene mutation and miR 21 expression in advanced stage colorectal cancer; The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients; APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction; APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation; In Taiwan downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation; APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC especially in patients with advanced disease;MicroRNA 21 promotes tumour malignancy via increased nuclear translocation of β catenin and predicts poor outcome in APC mutated but not in APC wild type colorectal cancer; However in our preliminary data the prognostic value of miR-21 levels was observed only in adenomatous polyposis coli APC-mutated tumours not in APC-wild-type tumours; We enrolled 165 colorectal tumour to determine APC mutation miR-21 levels and nuclear β-catenin expression by direct sequencing real-time PCR and immunohistochemistry	staging;	colorectal cancer;colorectal cancer
hsa-miR-21-5p	APH1B	-0.142759573450327	0.928848736959803	-1.21479604932868	0.00577560603326446	MirTarget	-0.290927209259053	1.62431064353651e-20		NA	NA	NA
hsa-miR-21-5p	APOLD1	-0.142759573450327	0.928848736959803	-0.376335247268241	0.452099926727468	miRNAWalker2_validate	-0.520450883320982	8.90831889443895e-38		NA	NA	NA
hsa-miR-21-5p	APPL1	-0.142759573450327	0.928848736959803	-0.182364369963587	0.748877584775604	miRNAWalker2_validate	-0.195099261510173	3.55854063868429e-22		NA	NA	NA
hsa-miR-21-5p	ARHGAP21	-0.142759573450327	0.928848736959803	0.95819478312162	0.0974454317312371	miRNAWalker2_validate	-0.30028897524233	5.81102696469372e-27		NA	NA	NA
hsa-miR-21-5p	ARHGAP24	-0.142759573450327	0.928848736959803	-0.252708946358773	0.451939433442859	MirTarget;miRNATAP	-0.312948652354249	2.89377453720354e-22		NA	NA	NA
hsa-miR-21-5p	ARHGEF12	-0.142759573450327	0.928848736959803	-0.465259998277415	0.493564105221691	miRNAWalker2_validate;MirTarget	-0.236174559762799	2.02871487295618e-18		NA	NA	NA
hsa-miR-21-5p	ARID4A	-0.142759573450327	0.928848736959803	-0.431374132649595	0.31434284133396	miRNAWalker2_validate	-0.303661902616789	4.46483636204476e-38		NA	NA	NA
hsa-miR-21-5p	ARMC8	-0.142759573450327	0.928848736959803	0.146504002609809	0.751309104347186	miRNATAP	-0.108180660940592	3.75734035519034e-12		NA	NA	NA
hsa-miR-21-5p	ARMCX1	-0.142759573450327	0.928848736959803	-0.436791088613953	0.353811733914516	MirTarget	-0.219371619018727	4.6249804997476e-10		NA	NA	NA
hsa-miR-21-5p	ART4	-0.142759573450327	0.928848736959803	-0.0951408842905228	0.804752167733841	mirMAP	-0.748328738243714	5.67447407910119e-23		NA	NA	NA
hsa-miR-21-5p	ATF2	-0.142759573450327	0.928848736959803	-0.0948181403041257	0.809110870210258	miRNAWalker2_validate	-0.231001727277097	1.50432513865861e-13		NA	NA	NA
hsa-miR-21-5p	ATM	-0.142759573450327	0.928848736959803	-0.0493046365018301	0.923235059785624	mirMAP	-0.230974664847653	3.0596374789676e-17	26289851	MiR-21 is an oncomiR that is overexpressed in nearly all cancers including ATC; Hence suppression of miR-21 could pave the way for ATC therapy		thyroid cancer
hsa-miR-21-5p	ATMIN	-0.142759573450327	0.928848736959803	0.278349878272978	0.624666473990518	miRNAWalker2_validate	-0.16057955550825	6.99755948412333e-20		NA	NA	NA
hsa-miR-21-5p	ATP11B	-0.142759573450327	0.928848736959803	0.921861162170949	0.0703985419573061	miRNAWalker2_validate	-0.186514892424103	5.53443040117541e-07		NA	NA	NA
hsa-miR-21-5p	ATP2B2	-0.142759573450327	0.928848736959803	1.3423290076799	7.91425381499571e-06	mirMAP	-0.264895951143355	0.000425195638483669		NA	NA	NA
hsa-miR-21-5p	ATP2B4	-0.142759573450327	0.928848736959803	0.0436927229652664	0.9479118704616	miRNAWalker2_validate;MirTarget	-0.269541901543463	1.44759461871948e-17		NA	NA	NA
hsa-miR-21-5p	ATRN	-0.142759573450327	0.928848736959803	-0.202106199091268	0.73488192704566	miRNATAP	-0.102465597038997	9.54242964779537e-08		NA	NA	NA
hsa-miR-21-5p	ATRX	-0.142759573450327	0.928848736959803	-0.308164370300245	0.600254457836354	miRNAWalker2_validate	-0.249265222441734	4.06831173161553e-22		NA	NA	NA
hsa-miR-21-5p	ATXN3	-0.142759573450327	0.928848736959803	0.0426741668165382	0.917385761602191	mirMAP	-0.169424226002042	4.68207024107888e-26		NA	NA	NA
hsa-miR-21-5p	AUTS2	-0.142759573450327	0.928848736959803	-0.0809599996915011	0.887890816008767	miRNAWalker2_validate	-0.109212629368038	0.000838210478213721		NA	NA	NA
hsa-miR-21-5p	B3GNT5	-0.142759573450327	0.928848736959803	2.59344243105562	1.64593587112362e-13	miRNAWalker2_validate	-0.309601402077663	7.56979187542241e-09		NA	NA	NA
hsa-miR-21-5p	BACE1	-0.142759573450327	0.928848736959803	0.000965412156275214	0.998488776933167	mirMAP	-0.185541233410245	2.62207018874446e-16		NA	NA	NA
hsa-miR-21-5p	BBS12	-0.142759573450327	0.928848736959803	-0.319306965117851	0.209978515065257	MirTarget	-0.225343894639492	1.99713954600638e-22		NA	NA	NA
hsa-miR-21-5p	BCL2	-0.142759573450327	0.928848736959803	-1.87954010585123	0.000984341696950829	miRNAWalker2_validate;miRTarBase	-0.486116466611641	4.94993012898121e-21	26555418;21468550;25994220;25381586;23359184;22964582;21376256	The expression of Bax Bcl-2 and miR-21 in parental and paclitaxel-resistant cells was detected by RT-PCR and Western blotting;BCL-2 up-regulation could be achieved by miR-21 overexpression which prevented T24 cells from apoptosis induced by doxorubicin; Furthermore the miR-21 induced BCL-2 up-regulation could be cancelled by the PI3K inhibitor LY294002;Meanwhile miR-21 loss reduced STAT3 and Bcl-2 activation causing an increase in the apoptosis of tumour cells in CAC mice;Changes in the sensitivity of osteosarcoma cells to CDDP were examined after transfection with miR-21 mimics or anti-miR-21 or bcl-2 siRNA in combination with CDDP;Resveratrol induces apoptosis of pancreatic cancers cells by inhibiting miR 21 regulation of BCL 2 expression; We also used Western blot to measure BCL-2 protein levels after down-regulation of miR-21 expression; Besides down-regulation of miR-21 expression can inhibit BCL-2 expression in PANC-1 CFPAC-1 and MIA Paca-2 cells; Over-expression of miR-21 expression can reverse down-regulation of BCL-2 expression and apoptosis induced by resveratrol; In this study we demonstrated that the effect of resveratrol on apoptosis is due to inhibiting miR-21 regulation of BCL-2 expression;Tumors harvested from these lungs have elevated levels of oncogenic miRNAs miR-21 and miR-155; are deficient for p53-regulated miRNAs; and have heightened expression of miR-34 target genes such as Met and Bcl-2;Bcl 2 upregulation induced by miR 21 via a direct interaction is associated with apoptosis and chemoresistance in MIA PaCa 2 pancreatic cancer cells; However the roles and mechanisms of miRNA miR-21 in regulation of Bcl-2 in pancreatic cancer remain to be elucidated; Then luciferase activity was observed after miR-21 mimics and pRL-TK plasmids containing wild-type and mutant 3'UTRs of Bcl-2 mRNA were co-transfected; Cells transfected with miR-21 inhibitor revealed an opposite trend. There was a significant increase in luciferase activity in the cells transfected with the wild-type pRL-TK plasmid in contrast to those transfected with the mutant one indicating that miR-21 promotes Bcl-2 expression by binding directly to the 3'UTR of Bcl-2 mRNA; Upregulation of Bcl-2 directly induced by miR-21 is associated with apoptosis chemoresistance and proliferation of MIA PaCa-2 pancreatic cancer cells	;;;;;;drug resistance	breast cancer;bladder cancer;colorectal cancer;sarcoma;pancreatic cancer;lung cancer;pancreatic cancer
hsa-miR-21-5p	BCL6	-0.142759573450327	0.928848736959803	0.0398285222796311	0.943105394101646	miRNAWalker2_validate	-0.304933247079297	5.43036581452504e-23		NA	NA	NA
hsa-miR-21-5p	BDH2	-0.142759573450327	0.928848736959803	0.010725310844899	0.981643402261982	miRNAWalker2_validate	-0.191806202169956	3.08631392543743e-13		NA	NA	NA
hsa-miR-21-5p	BMP2K	-0.142759573450327	0.928848736959803	-0.114393700530794	0.762090274860474	mirMAP	-0.199405442229406	5.64238989878735e-09		NA	NA	NA
hsa-miR-21-5p	BMP3	-0.142759573450327	0.928848736959803	0.122624699951704	0.765496662447771	MirTarget	-1.0000719728894	1.66064978529976e-28		NA	NA	NA
hsa-miR-21-5p	BMPR2	-0.142759573450327	0.928848736959803	-0.231734974429424	0.713753832463851	miRNAWalker2_validate;miRTarBase;MirTarget	-0.217097837256464	4.31156278024056e-20		NA	NA	NA
hsa-miR-21-5p	BOC	-0.142759573450327	0.928848736959803	1.08750723988247	0.0266495795145677	miRNAWalker2_validate	-0.369378666460681	2.09992979719832e-14		NA	NA	NA
hsa-miR-21-5p	BTBD3	-0.142759573450327	0.928848736959803	0.815930241503823	0.0929951069053313	miRNAWalker2_validate;miRNATAP	-0.133205941815074	9.42767603850498e-08		NA	NA	NA
hsa-miR-21-5p	BTBD7	-0.142759573450327	0.928848736959803	-0.200813881462332	0.680586072739571	miRNAWalker2_validate	-0.23946894228304	1.88953118249278e-29		NA	NA	NA
hsa-miR-21-5p	BTG2	-0.142759573450327	0.928848736959803	-1.49226033460123	0.044515682663653	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.271889515348514	4.86541229128609e-09	24821435	miR 21 regulates N methyl N nitro N' nitrosoguanidine induced gastric tumorigenesis by targeting FASLG and BTG2	tumorigenesis	gastric cancer
hsa-miR-21-5p	C16orf52	-0.142759573450327	0.928848736959803	-0.428280426828147	0.24715689353139	miRNATAP	-0.17489098935188	1.80280225638813e-19		NA	NA	NA
hsa-miR-21-5p	C1orf226	-0.142759573450327	0.928848736959803	-0.83799892619079	0.0862808022528832	mirMAP	-0.209671378953326	6.44200178894493e-05		NA	NA	NA
hsa-miR-21-5p	CADM2	-0.142759573450327	0.928848736959803	-2.36354384514459	1.18875938460695e-12	mirMAP;miRNATAP	-0.852795114758381	9.87753191981313e-24		NA	NA	NA
hsa-miR-21-5p	CALD1	-0.142759573450327	0.928848736959803	0.441360401737224	0.555181170053085	miRNAWalker2_validate;MirTarget	-0.114490339096093	0.000456521184701928		NA	NA	NA
hsa-miR-21-5p	CALN1	-0.142759573450327	0.928848736959803	-1.13844791757	0.00177546409753963	mirMAP	-0.499815411366113	8.63108549614904e-10		NA	NA	NA
hsa-miR-21-5p	CASC4	-0.142759573450327	0.928848736959803	-0.609228876487239	0.335335396270745	miRNATAP	-0.100117929548781	7.20011977519743e-06		NA	NA	NA
hsa-miR-21-5p	CASP10	-0.142759573450327	0.928848736959803	0.354559458104339	0.295897110426953	mirMAP	-0.182489722562965	2.9340743714182e-07		NA	NA	NA
hsa-miR-21-5p	CBL	-0.142759573450327	0.928848736959803	0.403788335503649	0.403074943038545	mirMAP	-0.219435783158958	6.50717000138084e-19		NA	NA	NA
hsa-miR-21-5p	CCDC121	-0.142759573450327	0.928848736959803	-0.152881660910574	0.515585171120125	MirTarget	-0.267949965844077	7.21203433284668e-36		NA	NA	NA
hsa-miR-21-5p	CCDC152	-0.142759573450327	0.928848736959803	-0.863122023206732	0.000165829117007392	mirMAP	-0.361400293113989	1.29299622016233e-20		NA	NA	NA
hsa-miR-21-5p	CCNG1	-0.142759573450327	0.928848736959803	-0.411811504148252	0.486275565047421	miRNAWalker2_validate	-0.351124695573807	1.0959074492675e-55		NA	NA	NA
hsa-miR-21-5p	CCNG2	-0.142759573450327	0.928848736959803	-1.09319477992261	0.0786773462384455	mirMAP	-0.100576766109129	0.00147226728207765		NA	NA	NA
hsa-miR-21-5p	CDK6	-0.142759573450327	0.928848736959803	1.59447860419636	0.000531332300056837	miRNAWalker2_validate;mirMAP	-0.197449989063307	3.4492907261557e-05		NA	NA	NA
hsa-miR-21-5p	CEP152	-0.142759573450327	0.928848736959803	0.70565835071091	0.0010472795292194	miRNAWalker2_validate	-0.123326725657416	3.18390296813198e-05		NA	NA	NA
hsa-miR-21-5p	CEP68	-0.142759573450327	0.928848736959803	-0.24960764645179	0.614173947080936	miRNATAP	-0.392295121745729	9.51485413821928e-90		NA	NA	NA
hsa-miR-21-5p	CEP97	-0.142759573450327	0.928848736959803	-0.224760188736034	0.393837188322952	miRNAWalker2_validate;MirTarget	-0.104490812571317	0.00271559146234075		NA	NA	NA
hsa-miR-21-5p	CLDN8	-0.142759573450327	0.928848736959803	0.662891196579784	0.112049837067746	MirTarget	-0.377817106851424	9.19914627852771e-05		NA	NA	NA
hsa-miR-21-5p	CLIC2	-0.142759573450327	0.928848736959803	0.296912682297645	0.41937380542498	MirTarget	-0.304444862008348	1.38670679779218e-18		NA	NA	NA
hsa-miR-21-5p	CLIP4	-0.142759573450327	0.928848736959803	1.12105361131789	0.00734000638854698	miRNAWalker2_validate	-0.479427264370382	1.10360006264809e-19		NA	NA	NA
hsa-miR-21-5p	CLOCK	-0.142759573450327	0.928848736959803	-0.110692370736752	0.760023195996913	miRNAWalker2_validate	-0.236507234922591	2.09692691035731e-08		NA	NA	NA
hsa-miR-21-5p	CNTFR	-0.142759573450327	0.928848736959803	0.586190265649432	0.147993787765382	miRNATAP	-1.1291637732312	3.37574589247041e-29		NA	NA	NA
hsa-miR-21-5p	COBLL1	-0.142759573450327	0.928848736959803	-0.234842154704134	0.620111634276767	miRNAWalker2_validate	-0.403337507435837	1.61465967789367e-27		NA	NA	NA
hsa-miR-21-5p	CPEB3	-0.142759573450327	0.928848736959803	-0.864927540042116	0.00231964486867428	miRNAWalker2_validate;MirTarget;miRNATAP	-0.335010077484093	7.2508091850588e-33		NA	NA	NA
hsa-miR-21-5p	CREBL2	-0.142759573450327	0.928848736959803	-0.549328377929987	0.345694685390556	miRNATAP	-0.28472177536481	8.10741363566585e-43		NA	NA	NA
hsa-miR-21-5p	CRIM1	-0.142759573450327	0.928848736959803	-0.30852804166978	0.634297036399116	miRNATAP	-0.601861660496863	2.85407244426875e-50		NA	NA	NA
hsa-miR-21-5p	CRTC3	-0.142759573450327	0.928848736959803	-0.193331660095702	0.720289772752545	mirMAP	-0.11499602708942	3.24511730045584e-13		NA	NA	NA
hsa-miR-21-5p	CSRNP3	-0.142759573450327	0.928848736959803	-0.299105566944235	0.371187975460312	miRNATAP	-0.727152198067792	2.6305143833534e-24		NA	NA	NA
hsa-miR-21-5p	CXXC4	-0.142759573450327	0.928848736959803	-1.30769268523275	5.95588414495972e-07	mirMAP	-0.257452952558405	3.19200115682289e-05		NA	NA	NA
hsa-miR-21-5p	CYBRD1	-0.142759573450327	0.928848736959803	-1.33008186622369	0.0757989845675171	miRNAWalker2_validate	-0.527905475921927	1.46100151801666e-27		NA	NA	NA
hsa-miR-21-5p	CYP4V2	-0.142759573450327	0.928848736959803	-0.467847164311658	0.304478426618107	miRNAWalker2_validate	-0.215885119351738	4.13077530023717e-13		NA	NA	NA
hsa-miR-21-5p	DAAM1	-0.142759573450327	0.928848736959803	-0.4294466447742	0.327925739625826	miRNAWalker2_validate	-0.280306110680269	1.3190405082497e-14		NA	NA	NA
hsa-miR-21-5p	DCP1A	-0.142759573450327	0.928848736959803	-0.134594395221957	0.774974308039499	miRNAWalker2_validate	-0.268749269182896	1.65793554093642e-51		NA	NA	NA
hsa-miR-21-5p	DDHD2	-0.142759573450327	0.928848736959803	-0.00445406947138149	0.993398840322419	miRNAWalker2_validate	-0.272751343136749	5.48298837525733e-15		NA	NA	NA
hsa-miR-21-5p	DDR2	-0.142759573450327	0.928848736959803	0.429153176166371	0.246426308689012	miRNAWalker2_validate	-0.617737869765056	4.17421223874419e-23		NA	NA	NA
hsa-miR-21-5p	DDX3X	-0.142759573450327	0.928848736959803	0.00464302591542243	0.995213268403161	miRNAWalker2_validate	-0.202520686411427	2.54629721847157e-32		NA	NA	NA
hsa-miR-21-5p	DLG1	-0.142759573450327	0.928848736959803	0.142566432178839	0.796362402050369	miRNAWalker2_validate	-0.145096003426752	1.51465636451203e-13		NA	NA	NA
hsa-miR-21-5p	DLGAP1	-0.142759573450327	0.928848736959803	0.531355879887816	0.124700178431523	miRNATAP	-0.283778868277711	0.00211859077742049		NA	NA	NA
hsa-miR-21-5p	DMD	-0.142759573450327	0.928848736959803	1.61734007179819	0.00016274582594595	miRNAWalker2_validate	-0.924101135044462	8.87022727088876e-52		NA	NA	NA
hsa-miR-21-5p	DMTF1	-0.142759573450327	0.928848736959803	0.0848878638046493	0.866059967776008	miRNAWalker2_validate	-0.11873640097588	2.02827189662546e-10		NA	NA	NA
hsa-miR-21-5p	DOCK4	-0.142759573450327	0.928848736959803	0.0682252033258575	0.864672472175758	miRNAWalker2_validate;miRTarBase	-0.217125422672835	3.28471037417372e-12		NA	NA	NA
hsa-miR-21-5p	DOCK7	-0.142759573450327	0.928848736959803	0.708571794514056	0.155659038414995	miRNAWalker2_validate;miRTarBase	-0.216996296670502	1.28480631069162e-15		NA	NA	NA
hsa-miR-21-5p	DSE	-0.142759573450327	0.928848736959803	0.157747779228511	0.744138338320756	miRNAWalker2_validate	-0.108893762885939	6.02880633523486e-05		NA	NA	NA
hsa-miR-21-5p	DST	-0.142759573450327	0.928848736959803	0.0159871038958519	0.982364261487702	mirMAP	-0.658195712040471	2.03281374044393e-48	22403704	While the EDCs and estrogen similarly altered the expression of multiple microRNAs in MCF-7 cells including miR-21 differential patterns of microRNA expression were induced by DDT and BPA compared to estrogen		breast cancer
hsa-miR-21-5p	DYNC1LI2	-0.142759573450327	0.928848736959803	0.171162172156333	0.788134364771302	miRNAWalker2_validate	-0.191465018085036	2.18459489707379e-25		NA	NA	NA
hsa-miR-21-5p	EGFR	-0.142759573450327	0.928848736959803	2.45303441219102	1.604711292626e-08	miRNAWalker2_validate;miRTarBase	-0.671030468978973	2.34536849626451e-18	24198203;20113523;24012640;24331411;26563758;19597153;26026961;20048743	In radically resected NSCLC patients the expression levels of miR-21 10b in patients with EGFR mutation were much higher than those without mutation;Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;Further the expression of miR-21 is regulated by EGFR via the activation of β-catenin and AP-1; These data indicate that a feedback loop exists between miR-21 and EGFR; These results clarify a novel association between miR-21 and EGFR in the regulation of cancer cell progression;MiR 21 overexpression is associated with acquired resistance of EGFR TKI in non small cell lung cancer;Higher expression levels of miR-21 AmiR-27a and miR-218 detected in this study suggest potential roles of these miRNAs in primary resistance to EGFR-TKI in advanced NSCLC patients with EGFR exon 19 deletion mutations;MiR 21 is an EGFR regulated anti apoptotic factor in lung cancer in never smokers; The changes in expression of some of these miRNAs including miR-21 were more remarkable in cases with EGFR mutations than in those without these mutations; In the never-smoker-derived lung adenocarcinoma cell line H3255 with mutant EGFR and high levels of p-EGFR and miR-21 antisense inhibition of miR-21 enhanced AG1478-induced apoptosis; In a never-smoker-derived adenocarcinoma cell line H441 with wild-type EGFR the antisense miR-21 not only showed the additive effect with AG1478 but also induced apoptosis by itself; These results suggest that aberrantly increased expression of miR-21 which is enhanced further by the activated EGFR signaling pathway plays a significant role in lung carcinogenesis in never-smokers as well as in smokers and is a potential therapeutic target in both EGFR-mutant and wild-type cases;Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR mutated lung cancer via nickel induced microRNA 21 expression;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status	;;progression;drug resistance;staging;drug resistance;tumorigenesis;;	lung squamous cell cancer;glioblastoma;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;lung cancer;lung cancer;glioblastoma
hsa-miR-21-5p	EIF1AX	-0.142759573450327	0.928848736959803	-0.00792306987407887	0.989503322245567	miRNATAP	-0.168398476195986	7.98842147428409e-23		NA	NA	NA
hsa-miR-21-5p	EIF4A2	-0.142759573450327	0.928848736959803	-0.080506845049694	0.917156856940229	miRNAWalker2_validate;miRTarBase	-0.220040692361575	2.91919563506492e-32		NA	NA	NA
hsa-miR-21-5p	EIF4EBP2	-0.142759573450327	0.928848736959803	0.103240469136134	0.883054720423375	miRNAWalker2_validate	-0.350566374017919	1.0351546276547e-72		NA	NA	NA
hsa-miR-21-5p	EIF5	-0.142759573450327	0.928848736959803	-0.152499705513117	0.82781735834554	miRNAWalker2_validate	-0.142310267973582	1.37631827729038e-16		NA	NA	NA
hsa-miR-21-5p	ELF2	-0.142759573450327	0.928848736959803	-0.154147963599649	0.74934632766984	MirTarget;miRNATAP	-0.171993160350573	1.0473729881449e-27		NA	NA	NA
hsa-miR-21-5p	ELOVL7	-0.142759573450327	0.928848736959803	1.00005373350157	0.00242046992066525	miRNAWalker2_validate	-0.23921022619148	9.19094815006121e-07		NA	NA	NA
hsa-miR-21-5p	EPHA4	-0.142759573450327	0.928848736959803	0.314306922484171	0.35597847837278	miRNAWalker2_validate;miRNATAP	-0.395716480858847	1.43468003643289e-18		NA	NA	NA
hsa-miR-21-5p	EPM2A	-0.142759573450327	0.928848736959803	0.169849242250371	0.54145392194126	miRNAWalker2_validate	-0.371811814361254	1.51752561454969e-59		NA	NA	NA
hsa-miR-21-5p	EPM2AIP1	-0.142759573450327	0.928848736959803	-0.555589739757937	0.270545315371848	mirMAP	-0.202953899150199	1.2516439307061e-16		NA	NA	NA
hsa-miR-21-5p	ESYT2	-0.142759573450327	0.928848736959803	0.427273752531192	0.525144548329114	miRNAWalker2_validate;MirTarget	-0.174303504358701	4.93947213585361e-21		NA	NA	NA
hsa-miR-21-5p	EXOC5	-0.142759573450327	0.928848736959803	0.0312232947252413	0.953700729780781	miRNAWalker2_validate	-0.136844263346474	1.3565987328826e-11		NA	NA	NA
hsa-miR-21-5p	FAM107B	-0.142759573450327	0.928848736959803	-0.1573662832586	0.791984170025001	miRNATAP	-0.110814609413332	0.000186371814630281		NA	NA	NA
hsa-miR-21-5p	FAM126B	-0.142759573450327	0.928848736959803	0.114368687988632	0.780286837074779	miRNAWalker2_validate	-0.216279509860791	3.86543497039112e-24		NA	NA	NA
hsa-miR-21-5p	FAM20B	-0.142759573450327	0.928848736959803	-0.0036053818736157	0.995298365029123	miRNAWalker2_validate	-0.103484094720083	4.53605224810891e-07		NA	NA	NA
hsa-miR-21-5p	FAM46A	-0.142759573450327	0.928848736959803	0.382863859677125	0.41443335814629	miRNAWalker2_validate;miRNATAP	-0.213449740798425	8.95844182792362e-10		NA	NA	NA
hsa-miR-21-5p	FAM63B	-0.142759573450327	0.928848736959803	-0.739632829976264	0.0195358049446134	miRNATAP	-0.349396451913809	6.76940380623322e-15		NA	NA	NA
hsa-miR-21-5p	FBXL17	-0.142759573450327	0.928848736959803	-0.916173174807771	0.0424731861781268	miRNAWalker2_validate;miRNATAP	-0.286204323216819	1.20316727680549e-31		NA	NA	NA
hsa-miR-21-5p	FBXO11	-0.142759573450327	0.928848736959803	0.217837316297667	0.695452893536808	miRNAWalker2_validate;miRNATAP	-0.150419868286549	2.90606997992055e-24		NA	NA	NA
hsa-miR-21-5p	FBXO3	-0.142759573450327	0.928848736959803	-0.381166580487077	0.424366611836505	miRNAWalker2_validate	-0.162176075054113	1.56719015700219e-16		NA	NA	NA
hsa-miR-21-5p	FCHO2	-0.142759573450327	0.928848736959803	-0.61314064445997	0.197553679638083	miRNATAP	-0.207844035881331	8.24692821106582e-19		NA	NA	NA
hsa-miR-21-5p	FERMT2	-0.142759573450327	0.928848736959803	0.0581179901478244	0.914921040157815	miRNAWalker2_validate	-0.395121625605477	1.35565362055128e-28		NA	NA	NA
hsa-miR-21-5p	FGD4	-0.142759573450327	0.928848736959803	0.323889464245984	0.391556378942234	MirTarget	-0.45517552665339	5.75049173554973e-51		NA	NA	NA
hsa-miR-21-5p	FGF1	-0.142759573450327	0.928848736959803	-0.646653213999114	0.101194218943267	MirTarget	-0.371477796734357	9.2806521720649e-15		NA	NA	NA
hsa-miR-21-5p	FGF2	-0.142759573450327	0.928848736959803	0.638662353674694	0.107063173352093	mirMAP	-1.0007316696677	9.45001605861665e-53		NA	NA	NA
hsa-miR-21-5p	FIGN	-0.142759573450327	0.928848736959803	1.65669188638741	2.00708412280046e-11	miRNAWalker2_validate	-0.632911145569647	4.11498669350143e-23		NA	NA	NA
hsa-miR-21-5p	FILIP1L	-0.142759573450327	0.928848736959803	-0.429543984885499	0.388606279703585	miRNAWalker2_validate	-0.239635659978444	1.14540694695997e-08		NA	NA	NA
hsa-miR-21-5p	FKBP5	-0.142759573450327	0.928848736959803	0.477470076361653	0.428166664898298	miRNAWalker2_validate	-0.185035856461726	0.000168306104029105		NA	NA	NA
hsa-miR-21-5p	FMN1	-0.142759573450327	0.928848736959803	-1.29178641840641	4.29779645688262e-08	miRNATAP	-0.157357633611186	0.0110643486482957		NA	NA	NA
hsa-miR-21-5p	FMOD	-0.142759573450327	0.928848736959803	-1.02107773383577	0.122507106815805	miRNAWalker2_validate;miRTarBase	-0.391125968145743	7.97182814844372e-14	23445447;19906824	Our method exhibits ultrahigh sensitivity toward miR-21 with detection limits of 10 fM at 37 °C and 1 aM at 4 °C which corresponds to nine strands of miR-21 in a 15 μL sample and it is capable of distinguishing among miRNA family members;Gain-of miR-21 function in MSMC and LSMC reduced TGF-beta-induced expression of fibromodulin and TGF-beta-induced factor P < 0.05 and moderately altered the rate of cell growth and caspase-3/7 activity in these cells	;	breast cancer;sarcoma
hsa-miR-21-5p	FOXN3	-0.142759573450327	0.928848736959803	0.221789312998601	0.691944744445288	miRNAWalker2_validate;mirMAP	-0.372367413731894	1.09993935350141e-34		NA	NA	NA
hsa-miR-21-5p	FOXP2	-0.142759573450327	0.928848736959803	-0.313083653537266	0.285169708352622	mirMAP	-0.695850593090754	1.03030535022016e-21		NA	NA	NA
hsa-miR-21-5p	FREM2	-0.142759573450327	0.928848736959803	1.54620234756943	8.08553956496661e-05	mirMAP	-0.30072670502533	0.00440939547495692		NA	NA	NA
hsa-miR-21-5p	FRMD3	-0.142759573450327	0.928848736959803	1.46760546538544	6.80178054736051e-09	MirTarget	-0.851710692588348	4.58036451272984e-40		NA	NA	NA
hsa-miR-21-5p	FZD4	-0.142759573450327	0.928848736959803	-0.757353288322241	0.186174262226182	mirMAP	-0.617423104849022	4.14640575991911e-50		NA	NA	NA
hsa-miR-21-5p	GALNT12	-0.142759573450327	0.928848736959803	0.969693105574259	7.24773523590065e-05	MirTarget	-0.161602797404253	0.00161251314485952		NA	NA	NA
hsa-miR-21-5p	GFOD1	-0.142759573450327	0.928848736959803	0.83022079484582	2.33534874434906e-05	mirMAP	-0.235487765626004	4.61516332759543e-10		NA	NA	NA
hsa-miR-21-5p	GFPT1	-0.142759573450327	0.928848736959803	0.167464555845039	0.797386887453203	mirMAP	-0.142526180324638	6.09235557381341e-08		NA	NA	NA
hsa-miR-21-5p	GLCCI1	-0.142759573450327	0.928848736959803	-1.0181212564435	0.0124653473541755	miRNAWalker2_validate;MirTarget;miRNATAP	-0.138403185382784	2.25008985423918e-05		NA	NA	NA
hsa-miR-21-5p	GLG1	-0.142759573450327	0.928848736959803	0.45635528927767	0.514635123563317	miRNAWalker2_validate	-0.103595106102721	1.60417528195648e-08		NA	NA	NA
hsa-miR-21-5p	GNAQ	-0.142759573450327	0.928848736959803	-0.390083881827332	0.502749535281732	miRNAWalker2_validate	-0.249840104809404	1.86559916333158e-19		NA	NA	NA
hsa-miR-21-5p	GNE	-0.142759573450327	0.928848736959803	0.327852706185205	0.476808361595283	miRNAWalker2_validate	-0.361917634353926	7.35979773519695e-38		NA	NA	NA
hsa-miR-21-5p	GNG2	-0.142759573450327	0.928848736959803	0.00481899103464212	0.990979066515865	miRNATAP	-0.40885794761468	1.88540041022037e-27		NA	NA	NA
hsa-miR-21-5p	GOLGA4	-0.142759573450327	0.928848736959803	-0.110926712205653	0.864187404975887	miRNAWalker2_validate	-0.222012915700454	8.41375733993621e-19		NA	NA	NA
hsa-miR-21-5p	GPAM	-0.142759573450327	0.928848736959803	-0.428988358030013	0.407710725661545	miRNAWalker2_validate;mirMAP	-1.00114277511959	1.84469265987155e-91		NA	NA	NA
hsa-miR-21-5p	GPD1L	-0.142759573450327	0.928848736959803	-1.54807541246761	0.00697147254770776	miRNAWalker2_validate	-0.34071263566115	1.68083569788086e-21		NA	NA	NA
hsa-miR-21-5p	GRAMD3	-0.142759573450327	0.928848736959803	0.58224712998688	0.203536908382137	MirTarget	-0.191692703974092	5.42500183145492e-09		NA	NA	NA
hsa-miR-21-5p	GRIK3	-0.142759573450327	0.928848736959803	-3.90622523996472	1.03313025762084e-19	mirMAP	-0.304631120717111	0.00129446770257955		NA	NA	NA
hsa-miR-21-5p	GTF2A1	-0.142759573450327	0.928848736959803	-0.309679596591037	0.277386744834285	miRNAWalker2_validate	-0.283152764965146	1.49524978983624e-08		NA	NA	NA
hsa-miR-21-5p	GTF2I	-0.142759573450327	0.928848736959803	0.086353080834809	0.89097551902727	miRNAWalker2_validate	-0.220348671606198	7.42280681170713e-12		NA	NA	NA
hsa-miR-21-5p	GXYLT1	-0.142759573450327	0.928848736959803	-0.173497976886448	0.68743573797504	mirMAP	-0.1211365090473	8.8863049905298e-10		NA	NA	NA
hsa-miR-21-5p	HBP1	-0.142759573450327	0.928848736959803	-0.296599016501406	0.630755997308	miRNATAP	-0.25898640261103	5.87675612636637e-57	26282675	MicroRNA 21 is a potential link between non alcoholic fatty liver disease and hepatocellular carcinoma via modulation of the HBP1 p53 Srebp1c pathway; Further studies revealed that Hbp1 was a novel target of microRNA-21 and a transcriptional activator of p53		liver cancer
hsa-miR-21-5p	HECTD1	-0.142759573450327	0.928848736959803	-0.237426326629886	0.716287800824104	miRNAWalker2_validate	-0.224768458276273	6.98871042038719e-23		NA	NA	NA
hsa-miR-21-5p	HERPUD2	-0.142759573450327	0.928848736959803	-0.0305460007730876	0.947856321634904	miRNAWalker2_validate	-0.105623791757372	2.47150913726809e-21		NA	NA	NA
hsa-miR-21-5p	HIPK1	-0.142759573450327	0.928848736959803	-0.236145315341239	0.683651118955056	mirMAP	-0.204008795337524	3.83283414565148e-18		NA	NA	NA
hsa-miR-21-5p	HIPK3	-0.142759573450327	0.928848736959803	-0.199528411313524	0.686452993939005	MirTarget	-0.521097587045661	3.6320364906261e-21		NA	NA	NA
hsa-miR-21-5p	HIVEP3	-0.142759573450327	0.928848736959803	-0.174796662039713	0.664218580836467	mirMAP	-0.129667316226954	2.58007847407097e-07		NA	NA	NA
hsa-miR-21-5p	HOXA9	-0.142759573450327	0.928848736959803	0.298313721458941	0.24004537666198	miRNAWalker2_validate	-0.504338752822271	9.99706963548109e-18		NA	NA	NA
hsa-miR-21-5p	HPS5	-0.142759573450327	0.928848736959803	0.263467612876401	0.560189451201812	miRNAWalker2_validate	-0.232535808424181	9.50607346505541e-30		NA	NA	NA
hsa-miR-21-5p	HSD17B4	-0.142759573450327	0.928848736959803	-1.11477579352742	0.0750847311095924	MirTarget	-0.158578636640685	7.31157763697113e-10		NA	NA	NA
hsa-miR-21-5p	IDS	-0.142759573450327	0.928848736959803	-0.314667833939453	0.635454975206594	mirMAP	-0.103158307050403	1.14782006532718e-06		NA	NA	NA
hsa-miR-21-5p	IL6R	-0.142759573450327	0.928848736959803	0.598657482221992	0.0587660178251148	MirTarget;miRNATAP	-0.329109136402319	6.05386734548003e-12		NA	NA	NA
hsa-miR-21-5p	IL6ST	-0.142759573450327	0.928848736959803	-2.17150435425912	0.000169593545858536	mirMAP	-0.56765013906967	2.31975560408847e-12		NA	NA	NA
hsa-miR-21-5p	ING3	-0.142759573450327	0.928848736959803	0.299651616504565	0.350579529899389	MirTarget	-0.281229141739032	1.71801122931191e-43		NA	NA	NA
hsa-miR-21-5p	INSR	-0.142759573450327	0.928848736959803	-0.196368931359925	0.747759955570247	mirMAP	-0.169950201921848	3.994162980573e-12	24804226;27220494;21618027	Downregulation of miR-21 in radioresistant NSCLC A549 cells inhibited the colony-forming ability and proliferation of A549 cells after IR; Moreover silencing miR-21 enhanced apoptosis of A549 cells induced by IR accompanied by decreased phosphorylated-Akt protein level; However PI3K activator IGF-1 reversed suppression of phosphorylated-Akt protein level and promotion of apoptosis of A549 cells after IR caused by miR-21 knockdown; Silencing miR-21 in radioresistant NSCLC A549 cells sensitized them to IR by inhibiting cell proliferation and enhancing cell apoptosis through inhibition of PI3K/Akt signaling pathway;In addition we also demonstrated that miR-21 confers decreased autophagy in cervical cancer cells after IR via the Akt-mTOR signaling pathway;In this study we found that miR-21 expression was upregulated in response to ionizing radiation IR in U251 cells which suggested that miR-21 could be involved in the response of U251 cells to radiation; We showed that a miR-21 inhibitor enhanced IR-induced glioblastoma cell growth arrest and increased the level of apoptosis which was probably caused by abrogation of the G2-M arrest induced by IR	;;drug resistance	lung squamous cell cancer;cervical and endocervical cancer;glioblastoma
hsa-miR-21-5p	IPO11	-0.142759573450327	0.928848736959803	-0.496082730300696	0.271588996549964	miRNATAP	-0.139705222942741	3.16755395368817e-13		NA	NA	NA
hsa-miR-21-5p	IPP	-0.142759573450327	0.928848736959803	-0.299491356477996	0.355384219772547	miRNAWalker2_validate	-0.211076693212558	5.31244386744071e-15		NA	NA	NA
hsa-miR-21-5p	IRAK3	-0.142759573450327	0.928848736959803	0.0854611760122777	0.815128043533673	mirMAP	-0.432949994242884	3.91318586050703e-18		NA	NA	NA
hsa-miR-21-5p	IREB2	-0.142759573450327	0.928848736959803	-0.136157700876868	0.797708154773672	miRNAWalker2_validate	-0.181574287669945	3.53247747736457e-17		NA	NA	NA
hsa-miR-21-5p	ISCU	-0.142759573450327	0.928848736959803	-0.337441238304292	0.555325685532506	miRNAWalker2_validate;miRTarBase	-0.14822383985227	1.75986348300615e-20		NA	NA	NA
hsa-miR-21-5p	ITGA1	-0.142759573450327	0.928848736959803	-0.379125772070542	0.41868297428497	mirMAP	-0.323092029358791	2.56001275037339e-17		NA	NA	NA
hsa-miR-21-5p	ITGA8	-0.142759573450327	0.928848736959803	-0.977345853015744	0.000199603681740399	mirMAP	-0.342105492128526	1.37371548465133e-06		NA	NA	NA
hsa-miR-21-5p	ITSN2	-0.142759573450327	0.928848736959803	0.21655538407562	0.670648407573523	miRNAWalker2_validate	-0.177512416339358	1.14681317030502e-18		NA	NA	NA
hsa-miR-21-5p	JAG1	-0.142759573450327	0.928848736959803	-0.0617885403839811	0.915463029420037	miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP	-0.129114979011453	1.3818320929806e-05		NA	NA	NA
hsa-miR-21-5p	JMY	-0.142759573450327	0.928848736959803	-0.253461265188045	0.57513695924078	miRNAWalker2_validate;mirMAP	-0.235903321169589	1.92593672468556e-23		NA	NA	NA
hsa-miR-21-5p	KBTBD6	-0.142759573450327	0.928848736959803	0.0778851689049915	0.845449661008944	miRNAWalker2_validate;MirTarget	-0.246852812805404	1.70175871699522e-30		NA	NA	NA
hsa-miR-21-5p	KBTBD7	-0.142759573450327	0.928848736959803	-0.230472299913127	0.40705790285638	miRNAWalker2_validate	-0.306212796610769	1.43100502382996e-15		NA	NA	NA
hsa-miR-21-5p	KCNA1	-0.142759573450327	0.928848736959803	-0.285937679368812	0.400718947761983	miRNATAP	-0.453580622436814	4.22573030564678e-08		NA	NA	NA
hsa-miR-21-5p	KCNK3	-0.142759573450327	0.928848736959803	-0.1498267916437	0.638553869587461	mirMAP	-0.61136642333281	8.80487917739721e-14		NA	NA	NA
hsa-miR-21-5p	KCTD12	-0.142759573450327	0.928848736959803	-0.00439952104008157	0.994421712160069	mirMAP	-0.386445789820893	1.03451712626426e-28		NA	NA	NA
hsa-miR-21-5p	KIAA0355	-0.142759573450327	0.928848736959803	0.0383586519626151	0.937641224961448	MirTarget	-0.215524473702999	5.29413237063212e-26		NA	NA	NA
hsa-miR-21-5p	KIAA1671	-0.142759573450327	0.928848736959803	0.126104551612677	0.832953703306877	mirMAP	-0.279928168579039	1.40901383280317e-24		NA	NA	NA
hsa-miR-21-5p	KLF12	-0.142759573450327	0.928848736959803	0.117265879839511	0.768557572249907	mirMAP	-0.108546469190554	0.000528302338249221		NA	NA	NA
hsa-miR-21-5p	KLF3	-0.142759573450327	0.928848736959803	-0.151389713462472	0.781608382019246	miRNATAP	-0.193891681199854	7.1580165334174e-23		NA	NA	NA
hsa-miR-21-5p	KLF5	-0.142759573450327	0.928848736959803	2.12661857395394	3.25791434701376e-06	miRNAWalker2_validate	-0.161191810211141	0.00817362097300997		NA	NA	NA
hsa-miR-21-5p	KLF6	-0.142759573450327	0.928848736959803	0.191416352652087	0.786924898738571	miRNATAP	-0.265706125768795	1.48123532644609e-18		NA	NA	NA
hsa-miR-21-5p	KLF7	-0.142759573450327	0.928848736959803	-0.117287832033727	0.629966654322436	mirMAP	-0.155847023803318	0.000243045740496368		NA	NA	NA
hsa-miR-21-5p	KLF9	-0.142759573450327	0.928848736959803	-0.545630887426171	0.325906951831897	miRNAWalker2_validate	-0.411775824457141	1.68311522946659e-33		NA	NA	NA
hsa-miR-21-5p	KLHL15	-0.142759573450327	0.928848736959803	0.405452396238188	0.239160719298304	miRNAWalker2_validate;miRNATAP	-0.334352113385367	3.56752848153276e-41		NA	NA	NA
hsa-miR-21-5p	KLHL24	-0.142759573450327	0.928848736959803	0.388290995184478	0.465314412339217	miRNAWalker2_validate	-0.246486780757483	4.3961308969267e-24		NA	NA	NA
hsa-miR-21-5p	KLHL4	-0.142759573450327	0.928848736959803	-0.828315610950711	0.000192604925634458	mirMAP	-0.498491500551846	8.9233572459027e-20		NA	NA	NA
hsa-miR-21-5p	KRIT1	-0.142759573450327	0.928848736959803	0.0712904045306173	0.879631799835983	miRNATAP	-0.1616071583699	1.04658447551898e-21		NA	NA	NA
hsa-miR-21-5p	KSR2	-0.142759573450327	0.928848736959803	-1.22962334316077	2.23333937111548e-07	mirMAP	-0.223089579565432	0.000225645228480768		NA	NA	NA
hsa-miR-21-5p	LAMA4	-0.142759573450327	0.928848736959803	-0.189925053375605	0.76228454506583	MirTarget	-0.249548681112465	8.17325028836981e-13		NA	NA	NA
hsa-miR-21-5p	LANCL1	-0.142759573450327	0.928848736959803	-0.333910203676936	0.560237995946526	MirTarget	-0.271771727767269	1.56622389244692e-40		NA	NA	NA
hsa-miR-21-5p	LANCL3	-0.142759573450327	0.928848736959803	1.96785554507882	1.41105262271356e-10	MirTarget	-0.133909445712517	0.0177934102483431		NA	NA	NA
hsa-miR-21-5p	LATS1	-0.142759573450327	0.928848736959803	-0.371662529365893	0.321471959809164	miRNAWalker2_validate	-0.341435277796525	1.60822085351667e-15	25769949	miR 21 modulates resistance of HR HPV positive cervical cancer cells to radiation through targeting LATS1; By using dual luciferase assay we verified a binding site between miR-21 and 3'-UTR of large tumor suppressor kinase 1 LATS1; Through direct binding miR-21 can regulate LATS1 expression in cervical cancer cells; LATS1 overexpression can reverse miR-21 induced higher colony formation rate and also reduced miR-21 induced S phase accumulation and G2/M phase block reduction under radiation treatment	drug resistance	cervical and endocervical cancer
hsa-miR-21-5p	LCOR	-0.142759573450327	0.928848736959803	-0.876291030543332	0.000314441070410498	mirMAP	-0.245810794918492	3.21079880165693e-06		NA	NA	NA
hsa-miR-21-5p	LCORL	-0.142759573450327	0.928848736959803	-0.145480663638923	0.39694194441754	miRNAWalker2_validate;miRNATAP	-0.202563121918904	1.56767259134752e-14		NA	NA	NA
hsa-miR-21-5p	LEMD3	-0.142759573450327	0.928848736959803	-0.206756245489048	0.656022611349937	miRNATAP	-0.143176877310001	9.35508155432617e-15		NA	NA	NA
hsa-miR-21-5p	LIFR	-0.142759573450327	0.928848736959803	-0.690645465433727	0.186441562718723	miRNAWalker2_validate	-0.845255051722729	1.67109223795086e-61		NA	NA	NA
hsa-miR-21-5p	LIMCH1	-0.142759573450327	0.928848736959803	-0.213596417990963	0.712551009110396	miRNAWalker2_validate	-0.430707850350701	1.03146170070126e-21		NA	NA	NA
hsa-miR-21-5p	LIN7C	-0.142759573450327	0.928848736959803	-0.082092699718272	0.860022447954396	miRNAWalker2_validate	-0.138450876623172	8.96827316055791e-15		NA	NA	NA
hsa-miR-21-5p	LNPEP	-0.142759573450327	0.928848736959803	-0.716636524937357	0.0351894471907604	mirMAP	-0.320312961380251	1.37367553783526e-12		NA	NA	NA
hsa-miR-21-5p	LONRF2	-0.142759573450327	0.928848736959803	-3.45738858653069	1.11871959084766e-13	miRNAWalker2_validate	-0.314063567115019	0.000127917502824947		NA	NA	NA
hsa-miR-21-5p	LPP	-0.142759573450327	0.928848736959803	0.0599000979079998	0.881653412887322	mirMAP	-0.346769488596522	2.26455556670428e-17		NA	NA	NA
hsa-miR-21-5p	LYRM7	-0.142759573450327	0.928848736959803	-0.393889484428689	0.297602013042825	miRNAWalker2_validate	-0.3290111222075	6.96813591950677e-59		NA	NA	NA
hsa-miR-21-5p	LZTFL1	-0.142759573450327	0.928848736959803	-0.923026483128237	0.0434981171600021	MirTarget	-0.185515103257871	8.98140010710389e-14		NA	NA	NA
hsa-miR-21-5p	MALT1	-0.142759573450327	0.928848736959803	0.509187283303175	0.230303118126528	miRNAWalker2_validate	-0.151618183797855	2.6958539808202e-11		NA	NA	NA
hsa-miR-21-5p	MAP3K2	-0.142759573450327	0.928848736959803	-0.0748363912158618	0.861499227946994	miRNAWalker2_validate;mirMAP	-0.117694966119235	0.000139697716851587		NA	NA	NA
hsa-miR-21-5p	MAPK14	-0.142759573450327	0.928848736959803	0.290920596126947	0.599673075907523	mirMAP	-0.113666961486213	5.43544541750221e-14		NA	NA	NA
hsa-miR-21-5p	MATN2	-0.142759573450327	0.928848736959803	-0.193450325575355	0.712979210380312	miRNATAP	-0.768197434664512	2.45662210651958e-35		NA	NA	NA
hsa-miR-21-5p	MBLAC2	-0.142759573450327	0.928848736959803	-0.693823332955858	0.0219160593106819	MirTarget	-0.30324709267087	2.58294121194424e-30		NA	NA	NA
hsa-miR-21-5p	MBNL1	-0.142759573450327	0.928848736959803	0.340748197787968	0.600958767860319	miRNAWalker2_validate;MirTarget;miRNATAP	-0.270694729308032	9.41739086394107e-36		NA	NA	NA
hsa-miR-21-5p	MBNL3	-0.142759573450327	0.928848736959803	0.699973468034472	0.00214957017186774	MirTarget;mirMAP	-0.505555147963686	1.12505269243851e-17		NA	NA	NA
hsa-miR-21-5p	MBP	-0.142759573450327	0.928848736959803	0.816676800281814	0.0807052537225863	mirMAP	-0.307335685655512	7.08494398545229e-18		NA	NA	NA
hsa-miR-21-5p	MCC	-0.142759573450327	0.928848736959803	-0.781952378283503	0.103278818597852	mirMAP	-0.323331426882474	2.97240228120409e-17		NA	NA	NA
hsa-miR-21-5p	MECP2	-0.142759573450327	0.928848736959803	-0.11260536644552	0.843968721153598	mirMAP	-0.143762623869606	4.46382340722961e-20		NA	NA	NA
hsa-miR-21-5p	MEF2A	-0.142759573450327	0.928848736959803	0.259524749916284	0.633330739734389	miRNAWalker2_validate	-0.239314536269004	2.95303977244974e-22		NA	NA	NA
hsa-miR-21-5p	MEF2C	-0.142759573450327	0.928848736959803	0.0448929221885477	0.9223949985253	miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP	-0.349922334680877	1.22856305351208e-30		NA	NA	NA
hsa-miR-21-5p	MEGF9	-0.142759573450327	0.928848736959803	-1.12364138077212	0.044925445718326	miRNAWalker2_validate;mirMAP;miRNATAP	-0.149252740775158	3.45279684976026e-05		NA	NA	NA
hsa-miR-21-5p	MEIS1	-0.142759573450327	0.928848736959803	-0.179967442130291	0.566545079449999	miRNAWalker2_validate	-0.188974454034122	5.73560587743375e-08		NA	NA	NA
hsa-miR-21-5p	MGA	-0.142759573450327	0.928848736959803	0.0760949401844631	0.879266930260868	miRNAWalker2_validate	-0.163741978014178	7.09173890086628e-11		NA	NA	NA
hsa-miR-21-5p	MIB1	-0.142759573450327	0.928848736959803	-0.0803908569254634	0.887930605033866	miRNAWalker2_validate;mirMAP	-0.133079244800936	7.70648522942277e-08		NA	NA	NA
hsa-miR-21-5p	MKNK2	-0.142759573450327	0.928848736959803	-0.509895636904107	0.459583955701971	miRNAWalker2_validate	-0.112053298236543	2.51599581837069e-05		NA	NA	NA
hsa-miR-21-5p	MLH3	-0.142759573450327	0.928848736959803	-0.336855419159494	0.417661120195489	mirMAP	-0.107851819719156	1.41645141081624e-10		NA	NA	NA
hsa-miR-21-5p	MOAP1	-0.142759573450327	0.928848736959803	-0.766302891095595	0.123144468385177	miRNAWalker2_validate	-0.158290848322491	5.34122665491176e-13		NA	NA	NA
hsa-miR-21-5p	MON2	-0.142759573450327	0.928848736959803	-0.498447967159882	0.34436127271318	miRNAWalker2_validate;MirTarget;mirMAP	-0.202563569965516	3.09016059395836e-23		NA	NA	NA
hsa-miR-21-5p	MORC3	-0.142759573450327	0.928848736959803	-0.146063499694996	0.762752750114629	miRNAWalker2_validate	-0.212646055021811	6.9942586544642e-24		NA	NA	NA
hsa-miR-21-5p	MPP5	-0.142759573450327	0.928848736959803	-0.205978141305087	0.666300087804681	miRNAWalker2_validate	-0.237239157137834	9.36639981799512e-24		NA	NA	NA
hsa-miR-21-5p	MPRIP	-0.142759573450327	0.928848736959803	0.232945010193403	0.70186038278276	miRNATAP	-0.157771533487089	1.49778101287334e-17		NA	NA	NA
hsa-miR-21-5p	MRAP2	-0.142759573450327	0.928848736959803	1.64763010314041	5.77268025287483e-10	miRNAWalker2_validate	-0.370285682421531	2.70370460520469e-07		NA	NA	NA
hsa-miR-21-5p	MSX1	-0.142759573450327	0.928848736959803	-0.548682883890978	0.0211533625927387	miRNATAP	-0.103509234308864	0.00763262040296447		NA	NA	NA
hsa-miR-21-5p	MTAP	-0.142759573450327	0.928848736959803	0.616820962602791	0.179911912916826	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.111192139316398	3.46052582192821e-05		NA	NA	NA
hsa-miR-21-5p	MTMR12	-0.142759573450327	0.928848736959803	0.339919720793683	0.541313253048783	miRNAWalker2_validate;miRNATAP	-0.24346929793164	2.16450805709664e-26		NA	NA	NA
hsa-miR-21-5p	MTMR3	-0.142759573450327	0.928848736959803	-0.121960879781944	0.828079420100376	mirMAP	-0.19915851983753	4.20986539364831e-26		NA	NA	NA
hsa-miR-21-5p	MTMR9	-0.142759573450327	0.928848736959803	-0.3548713489329	0.356803474476892	miRNAWalker2_validate	-0.209927277063899	5.02692538949849e-21		NA	NA	NA
hsa-miR-21-5p	MTR	-0.142759573450327	0.928848736959803	-0.00135196713302399	0.998022015634766	mirMAP	-0.125547989511482	1.35618621235181e-09		NA	NA	NA
hsa-miR-21-5p	MYC	-0.142759573450327	0.928848736959803	0.683231218937876	0.28627588440922	miRNAWalker2_validate	-0.270190862069706	5.76701615920235e-10	22072622	In contrast the levels of β-catenin TCF/LEF activity and the expression of c-Myc Cyclin-D which are increased in CSCs are also augmented in miR-21 overexpressing colon cancer cells accompanied by an increased sphere forming ability in vitro and tumor formation in SCID mice		colon cancer
hsa-miR-21-5p	MYCBP2	-0.142759573450327	0.928848736959803	0.0594810668923564	0.918817695424449	miRNAWalker2_validate	-0.379537407508662	1.36415926000751e-30		NA	NA	NA
hsa-miR-21-5p	MYO9A	-0.142759573450327	0.928848736959803	-0.22145795825728	0.575519392394261	miRNAWalker2_validate	-0.444675828361764	3.57171081828398e-23		NA	NA	NA
hsa-miR-21-5p	N4BP2L1	-0.142759573450327	0.928848736959803	-0.0784313933044056	0.834387479024389	MirTarget	-0.443625464430723	1.64635801690063e-44		NA	NA	NA
hsa-miR-21-5p	NAA30	-0.142759573450327	0.928848736959803	-0.317943720997047	0.468517998977097	miRNAWalker2_validate;MirTarget	-0.174052804733812	1.89838019591547e-20		NA	NA	NA
hsa-miR-21-5p	NBEA	-0.142759573450327	0.928848736959803	-1.96176842607598	1.57470607601836e-07	miRNAWalker2_validate;miRNATAP	-0.33294742747243	9.96623739729255e-10		NA	NA	NA
hsa-miR-21-5p	NDST1	-0.142759573450327	0.928848736959803	-0.312046909272991	0.653783786457178	mirMAP	-0.179688856545142	5.11311941039233e-16		NA	NA	NA
hsa-miR-21-5p	NEK1	-0.142759573450327	0.928848736959803	-0.401758695249633	0.30081530834483	miRNAWalker2_validate	-0.22844427453076	5.82636584669598e-22		NA	NA	NA
hsa-miR-21-5p	NFAT5	-0.142759573450327	0.928848736959803	0.248676186408694	0.656020689400483	miRNAWalker2_validate;mirMAP	-0.34259926440926	2.90805980799457e-41		NA	NA	NA
hsa-miR-21-5p	NFIA	-0.142759573450327	0.928848736959803	-1.05462646104845	0.0705553890079865	MirTarget;miRNATAP	-0.279890270029504	8.53158456489143e-12		NA	NA	NA
hsa-miR-21-5p	NFIB	-0.142759573450327	0.928848736959803	1.76125475020162	0.00522687999230436	miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP	-0.565176447004213	2.99659929184389e-28		NA	NA	NA
hsa-miR-21-5p	NFIC	-0.142759573450327	0.928848736959803	-0.529065853623083	0.295433633476229	mirMAP	-0.160493390478593	3.86214542438575e-07		NA	NA	NA
hsa-miR-21-5p	NHLRC2	-0.142759573450327	0.928848736959803	-0.31260188517604	0.236452791785795	mirMAP	-0.292427290079013	4.45068908877516e-16		NA	NA	NA
hsa-miR-21-5p	NHS	-0.142759573450327	0.928848736959803	-0.285088897594116	0.374938169151829	mirMAP	-0.125818575486007	0.00188922393602939	22776181	For the detection of mir21 capture probes and/or cell lysates were covalently attached onto the pencil graphite electrode PGE by coupling agents of N-dimethylaminopropyl-N'-ethylcarbodiimide hydrochloride EDC and N-hydroxysulfosuccinimide NHS		breast cancer
hsa-miR-21-5p	NIPAL2	-0.142759573450327	0.928848736959803	0.146443169080568	0.634863152550921	MirTarget	-0.207661042514944	1.92251727777557e-05		NA	NA	NA
hsa-miR-21-5p	NIPBL	-0.142759573450327	0.928848736959803	-0.222057067952046	0.710721587919304	miRNAWalker2_validate	-0.146219470940781	8.56025086385412e-12		NA	NA	NA
hsa-miR-21-5p	NKIRAS1	-0.142759573450327	0.928848736959803	-0.360632039236879	0.256302329457009	MirTarget	-0.215386848070623	1.12977727719919e-25		NA	NA	NA
hsa-miR-21-5p	NKTR	-0.142759573450327	0.928848736959803	-0.179015655602022	0.758299213484379	miRNAWalker2_validate	-0.209246344236617	6.0418343795957e-18		NA	NA	NA
hsa-miR-21-5p	NPAS3	-0.142759573450327	0.928848736959803	0.232187163336247	0.278433596669763	miRNATAP	-0.426485107404944	1.10681961838438e-17		NA	NA	NA
hsa-miR-21-5p	NR2C2	-0.142759573450327	0.928848736959803	0.16645267542736	0.739421092409797	miRNAWalker2_validate	-0.280718605434827	6.56731465445451e-20		NA	NA	NA
hsa-miR-21-5p	NTF3	-0.142759573450327	0.928848736959803	0.024874847085504	0.933203200546639	miRTarBase;miRNATAP	-0.481266108265449	4.86045305948914e-11		NA	NA	NA
hsa-miR-21-5p	NTRK2	-0.142759573450327	0.928848736959803	-0.506945780720691	0.383241401444281	mirMAP	-1.20592530757402	1.06429280476928e-57		NA	NA	NA
hsa-miR-21-5p	NTRK3	-0.142759573450327	0.928848736959803	0.647062771111165	0.0605869384885256	mirMAP	-0.741881394491742	6.39690202997752e-18		NA	NA	NA
hsa-miR-21-5p	NUBPL	-0.142759573450327	0.928848736959803	-0.82687526293607	0.0216068932208995	miRNAWalker2_validate	-0.216534064807805	5.34923384769218e-22		NA	NA	NA
hsa-miR-21-5p	NUDT16	-0.142759573450327	0.928848736959803	-0.642411989409529	0.181355051029713	mirMAP	-0.130900603359444	2.9323217792533e-10		NA	NA	NA
hsa-miR-21-5p	OSBPL1A	-0.142759573450327	0.928848736959803	0.103158481546703	0.854263033104893	miRNAWalker2_validate	-0.425153912368914	4.94510902429643e-54		NA	NA	NA
hsa-miR-21-5p	OSR1	-0.142759573450327	0.928848736959803	2.07814818458066	6.30396866343713e-08	miRNAWalker2_validate;MirTarget;miRNATAP	-0.670120706978658	1.59937973029098e-14		NA	NA	NA
hsa-miR-21-5p	OTUD1	-0.142759573450327	0.928848736959803	-0.143130660150977	0.721798696127425	miRNAWalker2_validate	-0.182699663266022	1.77150193510907e-18		NA	NA	NA
hsa-miR-21-5p	OTUD4	-0.142759573450327	0.928848736959803	0.172590235154609	0.736420468570913	mirMAP	-0.186108493333786	2.0478953360265e-16		NA	NA	NA
hsa-miR-21-5p	PAIP2B	-0.142759573450327	0.928848736959803	-0.283999392212889	0.37743470139167	miRNATAP	-0.368977512874348	3.28168682317723e-22		NA	NA	NA
hsa-miR-21-5p	PALM2	-0.142759573450327	0.928848736959803	1.51455602392483	6.79651678306911e-15	mirMAP	-0.180722451708013	0.000187496241366861		NA	NA	NA
hsa-miR-21-5p	PAN3	-0.142759573450327	0.928848736959803	-0.231322205467172	0.65932005797598	miRNAWalker2_validate;MirTarget	-0.160513810946105	3.14035145929224e-14		NA	NA	NA
hsa-miR-21-5p	PAPOLG	-0.142759573450327	0.928848736959803	0.27002364116338	0.467840744176523	mirMAP	-0.133272137049725	9.31455072221333e-12		NA	NA	NA
hsa-miR-21-5p	PBRM1	-0.142759573450327	0.928848736959803	-0.177917185887856	0.749044326514722	miRNAWalker2_validate	-0.244774891680869	4.784902197849e-28		NA	NA	NA
hsa-miR-21-5p	PCGF5	-0.142759573450327	0.928848736959803	-0.0880866742738178	0.865871559318486	mirMAP	-0.282044434420571	1.02711226243984e-34		NA	NA	NA
hsa-miR-21-5p	PDCD4	-0.142759573450327	0.928848736959803	-0.801621690533325	0.277927674055471	miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP	-0.109773096535706	0.00312356894215013	17991735;19308091;26169933;22547075;22086602;26212010;23052036;19633292;25566594;25575817;25316501;22072622;22267128;22322403;19836969;22212233;23604124;25520758;19682430;23272133;25477028;26252635;25994220;25991676;22747440;23827854;23824073;24865582;24902663;24888238;24149370;20978511;22689922;27355932;24331411;20372781;23558936;23201752;20815812;17968323;25310697;21842656;23567619;20581857;23888942;23548551;19013014;21685938;26142886;24472409;25973032;26418978;24659669;25543482;25543261;23904372;27725205;23564788;25400316;26150475;21546206;19509156;27035745;22353043;21412018;19276261;21330826;26864640;22335905;21636706	Programmed cell death 4 PDCD4 is an important functional target of the microRNA miR 21 in breast cancer cells;We also demonstrated that BMP-6-induced downregulation of miR-21 modified the activity of PDCD4 3'UTR and inhibited MDA-MB-231 cell invasion;The results indicated that the miR-21 inhibition could induce the cell cycle arrest in G1 phase upregulate the expression of Programmed Cell Death Protein 4 PDCD4 and thus active the caspase-3 apoptosis pathway;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;The effects were accompanied by up-regulation of tumor suppressor PDCD4 a known miR-21 target; as well as up-regulation of cofilin and profilin two key proteins involved in actin polymerization and cytoskeleton maintenance as a consequence of miR-182 down-regulation;Increased levels of miR-21 in turn downregulate the expression of the metastasis-suppressor protein programmed cell death 4 PDCD4 a validated miR-21 target;Fenhexamid and fludioxonil stimulated miR-21 expression in a concentration-dependent manner and reduced the expression of miR-21 target Pdcd4 protein; Antisense to miR-21 blocked the increase in Pdcd4 protein by fenhexamid and fludioxonil;This process leads to microRNA-21 miR-21 production and a tumor suppressor protein e.g PDCD4 program cell death 4 reduction; Transfection of MCF-7 cells with PKCepsilon or Nanog-specific small interfering RNAs effectively blocks HA-mediated PKCepsilon-Nanog signaling events abrogates miR-21 production and increases PDCD4 expression/eIF4A binding;Through dual-luciferase method it was verified that PDCD4 and PDCD10 were real target genes of miR-21 and miR-200c respectively;We identified miR-21 was directly downregulated by RBP2 and found that miR-21 downregulated PDCD4 expression in leukemia cells;MTCs were consistently associated with miR-21 up-regulation P < .0016 and featured significant PDCD4 nuclear down-regulation; An inverse correlation emerged between miR-21 overexpression and PDCD4 down-regulation P = .0013; This study showed in MTCs that miR-21 regulates PDCD4 expression and also that the miR-21/PDCD4 pathway correlates with clinicopathological variables and prognosis;Although microRNA-21 miR-21 is emerging as an oncogene and has been shown to target several tumor suppressor genes including programmed cell death 4 PDCD4 its precise mechanism of action on cancer stem cells CSCs is unclear; Herein we report that FOLFOX-resistant HCT-116 and HT-29 cells that are enriched in CSCs show a 3- to 7-fold upregulation of pre- and mature miR-21 and downregulation of PDCD4; Likewise overexpression of miR-21 in HCT-116 cells achieved through stable transfection led to the downregulation of PDCD4 and transforming growth factor beta receptor 2 TGFβR2;Prognostic significance of PDCD4 expression and association with microRNA 21 in each Dukes' stage of colorectal cancer patients; Furthermore relationships between the PDCD4 mRNA and microRNA-21 miR-21 were evaluated; The PDCD4 mRNA was investigated by the quantitative real-time RT-PCR method and miR-21 was examined by TaqMan microRNA assays; Significant inverse correlations were demonstrated between PDCD4 and miR-21;miR 21 promotes migration and invasion by the miR 21 PDCD4 AP 1 feedback loop in human hepatocellular carcinoma; The expression level of miR-21 was inversely correlated with the protein expression level of a previously identified target gene programmed cell death 4 PDCD4;This short review will focus on our recent finding that the microRNA miR-21 posttranscriptionally regulates Pdcd4 as well as invasion intravasation and metastasis;PDCD4 expression inversely correlated with miR 21 levels in gastric cancers;HBx mediated miR 21 upregulation represses tumor suppressor function of PDCD4 in hepatocellular carcinoma; HBx downregulates PDCD4 expression at least partially through miR-21; Taken together this study reported for the first time that HBx downregulates PDCD4 and upregulates miR-21 expression; The deregulation of PDCD4 by HBx through miR-21 represents a potential novel mechanism of the downregulation of PDCD4 in HBV-related HCC and provides new insights into HCC development;MicroRNA 21 Down regulates Rb1 Expression by Targeting PDCD4 in Retinoblastoma; MicroRNA-21 miR-21 possesses the oncogenic potential to target several tumor suppressor genes including PDCD4 and regulates tumor progression and metastasis; However the mechanism of how miR-21 regulates PDCD4 is poorly understood in RB; Using the TargetScan program we identified a list of potential target genes of these miRNAs of which PDCD4 is one the targets of miR-21; In this study we focused on the regulatory mechanism of miR-21 on PDCD4 in RB; We demonstrated an inverse correlation between miR-21 and PDCD4 expression in Weri-Rb1 and Y79 cells; These data suggest that miR-21 down-regulates Rb1 by targeting PDCD4 tumor suppressor;MicroRNA 21 promotes cell proliferation and down regulates the expression of programmed cell death 4 PDCD4 in HeLa cervical carcinoma cells; In this study we found that the inhibition of miR-21 in HeLa cervical cancer cells caused profound suppression of cell proliferation and up-regulated the expression of the tumor suppressor gene PDCD4;Additionally resveratrol increased the expression of tumor suppressors PDCD4 and maspin which are negatively regulated by miR-21; Short interfering si RNA against PDCD4 attenuated resveratrol's effect on prostate cancer cells and similar effects were observed following over expression of miR-21 with pre-miR-21 oligonucleotides; MiR-21 expression in these cells appeared to be dependent on Akt as LY294002 reduced the levels of miR-21 along with a concurrent increase in PDCD4 expression; These tumor- and metastatic-suppressive effects of resveratrol were associated with reduced miR-21 and pAkt and elevated PDCD4 levels;The expression of miR-21 and PDCD4 mRNA in transfected cells was quantified by real-time polymerase chain reaction and the expression of PDCD4 protein by Western blot; Cell proliferation apoptosis migration and invasion assays were performed to determine the biological effects of miR-21 expression and PDCD4 inhibition; Co-transfection of miR-21-sponge with PDCD4 siRNA upregulated miR-21 expression in these cells; PDCD4 mRNA and protein levels were increased 2.14-fold and 2.16-fold respectively following inhibition of miR-21 expression; Depletion of PDCD4 by siRNA transfection reversed the reduction of cell proliferation migration and invasion induced by inhibition of miR-21 in A549 cells; It indicates that miR-21 is highly expressed in patients with NSCLC and inhibition of miR-21 expression reduces proliferation migration and invasion of A549 cells by upregulating PDCD4 expression; Modulation of miR-21 or PDCD4 expression may provide a potentially novel therapeutic approach for NSCLC;IL 6 Inhibits the Targeted Modulation of PDCD4 by miR 21 in Prostate Cancer; In this study we evaluated the role of IL-6 in PDCD4 gene expression and how the microRNA miR-21 regulates this process in prostate cancer cell lines PC-3 and LNCaP; The expression of PDCD4 was inhibited by IL-6 but this effect was weakened in cell lines with low expression of miR-21; These findings support the feasibility of future efforts for diagnosis and gene therapy for prostate cancer that are based on IL-6 miR-21 and PDCD4;Furthermore the absence of miR-21 increased the expression of its target gene PDCD4 and subsequently modulated nuclear factor NF-κB activation;In this study using reverse phase protein arrays RPPAs we assessed MPS1 mediated cell signaling pathways and demonstrated that inhibiting MPS1 could upregulate the expression of the tumor suppressor PDCD4 and MSH2 genes by down regulating micro RNA-21 miR-21; We also show the prognostic effect of miR21 PDCD4 and MSH2 levels to patient survival across different GBM molecular subtypes;We also analyzed the immunohistochemical expression of PDCD4 an miR-21 gene target; PDCD4 expression was significantly downregulated in MTC samples consistent with miR-21 upregulation;Aldose reductase inhibition suppresses colon cancer cell viability by modulating microRNA 21 mediated programmed cell death 4 PDCD4 expression; Further AR inhibition also increased PDCD4 a putative target of miR-21 in human colon cancer cells; Collectively these results indicate that AR inhibition prevents growth factor-induced colon cancer growth by down-regulating miR-21 expression and increasing PDCD4 levels through the reactive oxygen species ROS/AMPK/mTOR/AP1/4E-BP1 pathway;Berberine could inhibit miR-21 expression and function in ovarian cancer as shown by an enhancement of its target PDCD4 an important tumor suppressor in ovarian cancer;Overexpression of miR-21 in cisplatin sensitive cells decreased PDCD4 levels and increased cell proliferation;MicroRNA 21 miR 21 post transcriptionally downregulates tumor suppressor PDCD4 and promotes cell transformation proliferation and metastasis in renal cell carcinoma; MiR-21 induces neoplastic transformation cell proliferation and metastasis and downregulates programmed cell death4 PDCD4 in some cancers; The aim of this study was to investigate the roles and interactions of PDCD4 and miR-21 in human renal cell carcinoma RCC; The expression levels of PDCD4 protein and mRNA and miR-21 were examined by Western blot analysis and by qRT-PCR and luciferase reporter assays; MiR-21 not only promoted cancer cell hyperplasia and contributed to tumor cell transformation and metastasis but also post-transcriptionally downregulated PDCD4 protein expression; PDCD4 and miR-21 expression levels potentially play an important role in renal cell cancer;Programmed cell death 4 PDCD4 is a tumor suppressor gene whose expression is directly controlled by microRNA-21 miR-21; This PDCD4 and miR-21 inverse expression was also noted in cells and exosomes from OSC peritoneal effusions compared with nonneoplastic effusions; PDCD4 and miR-21 are involved in OSC oncogenesis;Finally in a subgroup of tumor specimens ROC curve analysis performed on quantitative PCR data sets for miR-21 ITGβ4 and PDCD4 shows that the combination of high miR-21 with low ITGβ4 and PDCD4 expression is able to predict presence of metastasis; In conclusion miR-21 is a key player in oncogenic EMT its overexpression is controlled by the cooperation of genetic and epigenetic alterations and its levels along with ITGβ4 and PDCD4 expression could be exploited as a prognostic tool for CRC metastasis;Transfection of anti-miR-21 rendered HCC cells sensitive to IFN-α/5-FU and such sensitivity was weakened by transfection of siRNAs of target molecules PETN and PDCD4 miR-21 expression in clinical HCC specimens was significantly associated with the clinical response to the IFN-α/5-FU combination therapy and survival rate;In ZD esophagus and tongue oncogenic miR-31 and miR-21 overexpression was accompanied by down-regulation of their respective tumor-suppressor targets PPP2R2A and PDCD4;Due to inhibition of miR-21 expression of the programed cell death 4 PDCD4 gene was promoted in tumors resulting in the induction of apoptosis of tumor cells;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;Clinicopathological and prognostic significance of PDCD4 and microRNA 21 in human gastric cancer; Recent studies have demonstrated that the novel tumor suppressor protein programmed cell death 4 PDCD4 is downregulated in several human solid cancer types and is suppressed by microRNA-21 miR-21; The objectives of this study were: i to establish the clinicopathological and prognostic significance of PDCD4 mRNA and ii to elucidate any correlation between PDCD4 mRNA and miR-21 in gastric cancer; We also performed an association study comparing PDCD4 mRNA and miR-21 in eight cell lines and 49 gastric cancers; An inverse correlation between PDCD4 mRNA and miR-21 was found in gastric cancer; Furthermore our findings suggest that PDCD4 mRNA is negatively regulated by miR-21 in gastric cancer and may serve as a target for effective therapies;Furthermore miR-21 mimic or inhibitor significantly reduced or increased the expression of PDCD4 and TPM1; MiR-21 is overexpressed in RCC tissue and modulates the growth apoptosis and cell cycle progression of RCC cells and regulates the expression of PDCD4 and TPM1;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;Curcumin treatment reduced miR-21 promoter activity and expression in a dose-dependent manner by inhibiting AP-1 binding to the promoter and induced the expression of the tumour suppressor Pdcd4 programmed cell death protein 4 which is a target of miR-21; Taken together this is the first paper to show that curcumin inhibits the transcriptional regulation of miR-21 via AP-1 suppresses cell proliferation tumour growth invasion and in vivo metastasis and stabilizes the expression of the tumour suppressor Pdcd4 in colorectal cancer;MicroRNA 21 miR 21 post transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion intravasation and metastasis in colorectal cancer; This is the first study to show that Pdcd4 is negatively regulated by miR-21;Inflammation and MiR 21 pathways functionally interact to downregulate PDCD4 in colorectal cancer; These findings indicate that miR-21 is a component of the COX-2 inflammation pathway and that this pathway promotes worsening of disease stage in colorectal cancer by inducing accumulation of PGE2 and increasing expression of miR-21 with consequent downregulation of the tumour suppressor gene PDCD4;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Interestingly NVP-LDE-225 induced PDCD4 and apoptosis and inhibited cell viability by suppressing miR-21;PDCD4 a direct target gene of miR-21 could mediate chemoresistance to docetaxel in PC3 cells;Oxidative stress upregulates PDCD4 expression in patients with gastric cancer via miR 21; The expression of miR-21 and PDCD4 was highly correlated with the degree of differentiation tumor staging local lymphatic node metastasis and remote metastasis; In addition the relative expression of PDCD4 was negatively correlated with miR-21; Thus we conclude that ROS promotes gastric carcinogenesis via upregulating miR-21 expression which in turn down-regulates the expression of PDCD4 in gastric cancer cells;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;MicroRNA 21 down regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G; Expression of PDCD4 protein correlates inversely with expression of miR-21 in a number of human glioblastoma cell lines such as T98G A172 U87 and U251; Inhibition of miR-21 increases endogenous levels of PDCD4 in cell line T98G and over-expression miR-21 inhibits PDCD4-dependent apoptosis;Treatment of HSC-3 cells with Nanog- and/or Stat-3-specific small interfering RNAs effectively blocks HA-mediated Nanog-Stat-3 signaling events abrogates miR-21 production and increases PDCD4 expression;Programmed cell death 4 PDCD4 is critical in mediating apoptosis in GBM and is downregulated by miR-21 which may mediate the resistance of glioblastoma cells against chemotherapy or radiation via its target genes PDCD4; This review will focus on the roles of miRNAs family members particularly miR-21 and its target gene PDCD4 in tumors like glioblastoma and new targeting strategies as examples some new targeting therapeutic methods and molecular mechanisms of signal pathways in glioblastoma therapeutics to give the reader the current trends of approach to target regulation of these miRNA and genes for future glioma therapies;Using Western blot analysis miR-21 knockdown enhanced the expression of tumor suppressor PDCD4 and attenuated apoptosis inhibitor c-IAP2;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;METHODS 3-45-dimethylthiazol-2-yl-25-diphenyltetrazolium bromide MTT assay was used to determine the effect of 5-FU on the viability of RKO cells with knockout of miR-21 or high expression of PDCD4;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;In this study we analyzed the expression of miR-21 and of its target PDCD4 Programmed Cell Death 4 during normal hematopoietic differentiation and in AMLs;MiR 21 mediates the radiation resistance of glioblastoma cells by regulating PDCD4 and hMSH2; In addition miR-21 knockdown increased the expression of endogenous PDCD4 and hMSH2 which contributed to the apoptosis and G2 arrest of T98G cells; The findings suggested that miR-21 may mediate the resistance of glioblastoma cells against radiation via its target genes PDCD4 and hMSH2;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated;The expression of programmed cell death-4 PDCD4 was increased by I3C and reduced by miR-21 transfection; Such enhanced chemosensitivity might be explained by the increased expression of PDCD4 which is a downstream target which miR-21 negatively regulates;It has been reported that miR-21 was upregulated in most malignant cancers the proposed mechanism of which was through suppressing expression of programmed cell death 4 PDCD4; In present study it is firstly reported that miR-21 was upregulated in Kazakh's ESCC and that miR-21 played a negative role in regulating PDCD4 using in situ hybridization ISH and luciferase reporter approach;To measure levels of microRNA miR-21 and its target gene programmed cell death 4 PDCD4 in samples of human cutaneous malignant melanoma and normal non-malignant control skin; Relative levels of miR-21 and PDCD4 mRNA were measured using a quantitative real-time reverse transcription-polymerase chain reaction; Compared with normal skin samples the relative level of miR-21 was significantly higher and the relative level of PDCD4 mRNA was significantly lower in the melanoma specimens; A significant negative correlation between PDCD4 mRNA and miR-21 was demonstrated in malignant melanoma r = -0.602; Elevated miR-21 and reduced PDCD4 mRNA levels were both significantly correlated with increased tumour size a higher Clark classification level and the presence of lymph node metastases in malignant melanoma; These findings suggest that miR-21 and PDCD4 might be potential biomarkers for malignant melanoma and might provide treatment targets in the future;MicroRNA 21 and PDCD4 expression in colorectal cancer; Studies have shown that miR-21 exerts its oncogenic activity by targeting the PDCD4 tumour suppressor 3'-UTR; The purpose of this study was to delineate the interaction between PDCD4 and its oncogenic modulator miR-21 in colorectal cancer; A cohort of 48 colorectal tumours 61 normal tissues and 7 polyps were profiled for miR-21 and PDCD4 gene expression; A significant inverse relationship between miR-21 and PDCD4 gene expression p < 0.001 was identified by RT-qPCR; In addition significant reduction of PDCD4 p < 0.001 expression and reciprocal upregulation of miR-21 p = 0.005 in a progressive manner from tumour-polyp-normal mucosae was identified; This study demonstrates the inverse relationship between miR-21 and PDCD4 thus suggesting that miR-21 post-transcriptionally modulates PDCD4 via mRNA degradation;Expression patterns of miR-21 RNA and its target tumor suppressor protein PDCD4 were mutually exclusive;Exosome shuttling microRNA 21 promotes cell migration and invasion targeting PDCD4 in esophageal cancer;The expression of programmed cell death 4 PDCD4 which is a miR-21 targeting apoptotic gene has also been enhanced by UA; And over-expression of miR-21 in U251 cells abolished the enhancement of PDCD4 protein by UA;miR-21 and miR-155 expression was assessed in tumor tissue and in adjacent normal tissue of 156 CRC patients by TaqMan MicroRNA assays and PDCD4 and TP53INP1 mRNA levels were measured by quantitative real-time reverse transcriptase PCR RT-PCR; Significant inverse correlations were demonstrated between miR-21 and PDCD4 mRNA and miR-155 and TP53INP1 mRNA;The aim of this study was a to determine a role of microRNA-21 in esophageal squamous cell carcinoma and b to elucidate the regulation of the programmed cell death 4 PDCD4 gene by microRNA-21; In addition the regulation of PDCD4 by microRNA-21 was elucidated to identify the mechanisms of this regulation; The PDCD4 protein levels in esophageal squamous cell carcinoma cells have an inverse correlation with microRNA-21 expression; MicroRNA-21 targets PDCD4 at the posttranscriptional level and regulates cell proliferation and invasion in esophageal squamous cell carcinoma;Maspin Pdcd4 and miR-21 expressions were evaluated by a real-time polymerase chain reaction in 20 endometrial cancer and 10 normal endometrium samples; Comparison between IA and more advanced International Federation of Gynecology and Obstetrics stages of endometrial cancer in regard to expression levels of maspin Pdcd4 and miR-21 did not reveal any significant differences;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;Sequence prediction and gene expression regulation assays showed that miR-21 could reversely regulate the expression of PDCD4 P < 0.01; Suppression of miR-21 expression is associated with an elevation of Pdcd4 resulting in a significant reduction of proliferation and the apoptosis rate 2.6% was increased to 10.9%; Moreover the anti-proliferation and pro-apoptotic effect by miR-21 suppression could be reversed by PDCD4 knock down;Downregulation of Pdcd4 by mir 21 facilitates glioblastoma proliferation in vivo; We demonstrate that downregulation of mir-21 in glioblastoma-derived cell lines results in increased expression of its target programmed cell death 4 Pdcd4 a known tumor-suppressor gene; In addition our data indicate that either downregulation of mir-21 or overexpression of its target Pdcd4 in glioblastoma-derived cell lines leads to decreased proliferation increased apoptosis and decreased colony formation in soft agar; Using a glioblastoma xenograft model in immune-deficient nude mice we observe that glioblastoma-derived cell lines in which mir-21 levels are downregulated or Pdcd4 is over-expressed exhibit decreased tumor formation and growth; These critical in vivo findings demonstrate an important functional linkage between mir-21 and Pdcd4 and further elucidate the molecular mechanisms by which the known high level of mir-21 expression in glioblastoma can attribute to tumorigenesis--namely inhibition of Pdcd4 and its tumor-suppressive functions	;;;;;metastasis;;;;;worse prognosis;;staging;;metastasis;;;metastasis;progression;;;;;;poor survival;;;;;metastasis;;metastasis;poor survival;drug resistance;;;drug resistance;;progression;;metastasis;metastasis;staging;;;drug resistance;staging;metastasis;differentiation;tumorigenesis;;;;drug resistance;;;;;;differentiation;drug resistance;;;malignant trasformation;malignant trasformation;metastasis;progression;;cell migration;;;;staging;drug resistance;;tumorigenesis	breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;chronic myeloid leukemia;thyroid cancer;colon cancer;colorectal cancer;liver cancer;colon cancer;gastric cancer;liver cancer;retinoblastoma;cervical and endocervical cancer;prostate cancer;lung squamous cell cancer;prostate cancer;colorectal cancer;glioblastoma;thyroid cancer;colon cancer;ovarian cancer;ovarian cancer;kidney renal cell cancer;ovarian cancer;colorectal cancer;liver cancer;esophageal cancer;glioblastoma;lung squamous cell cancer;gastric cancer;kidney renal cell cancer;glioblastoma;colorectal cancer;colorectal cancer;colorectal cancer;lung cancer;prostate cancer;prostate cancer;gastric cancer;B cell lymphoma;glioblastoma;head and neck cancer;glioblastoma;ovarian cancer;liver cancer;colon cancer;gastric cancer;B cell lymphoma;acute myeloid leukemia;glioblastoma;prostate cancer;pancreatic cancer;esophageal cancer;melanoma;colorectal cancer;colorectal cancer;esophageal cancer;glioblastoma;colorectal cancer;esophageal cancer;endometrial cancer;pancreatic cancer;lung squamous cell cancer;glioblastoma
hsa-miR-21-5p	PDE1C	-0.142759573450327	0.928848736959803	1.48359346390131	8.76026381793182e-07	mirMAP	-0.88293003236443	1.44419962390607e-29		NA	NA	NA
hsa-miR-21-5p	PDGFD	-0.142759573450327	0.928848736959803	-1.11557097366064	0.0133449965972019	miRNAWalker2_validate	-0.58246612445279	1.38968424696146e-42		NA	NA	NA
hsa-miR-21-5p	PELI1	-0.142759573450327	0.928848736959803	1.40035453427595	0.00328592797249891	miRNAWalker2_validate;MirTarget;miRNATAP	-0.349049524751964	1.76483309884008e-25		NA	NA	NA
hsa-miR-21-5p	PER2	-0.142759573450327	0.928848736959803	-0.658193861382429	0.186008457103567	MirTarget	-0.357383948326546	8.76797882197739e-37		NA	NA	NA
hsa-miR-21-5p	PER3	-0.142759573450327	0.928848736959803	-0.30724409225877	0.52011400007181	miRNAWalker2_validate	-0.427279087231266	2.59821833454111e-34		NA	NA	NA
hsa-miR-21-5p	PEX5L	-0.142759573450327	0.928848736959803	-1.62962419699106	8.07720056122242e-06	mirMAP	-0.42348748755714	2.1841515323527e-07		NA	NA	NA
hsa-miR-21-5p	PGRMC2	-0.142759573450327	0.928848736959803	-0.397850982606184	0.512176002802529	miRNAWalker2_validate	-0.166401308644768	1.0800195721174e-15		NA	NA	NA
hsa-miR-21-5p	PHACTR2	-0.142759573450327	0.928848736959803	0.295695508944888	0.503603318747921	miRNAWalker2_validate	-0.271937515832	4.74116250555523e-18		NA	NA	NA
hsa-miR-21-5p	PHIP	-0.142759573450327	0.928848736959803	0.0779847721341262	0.890568785404578	miRNAWalker2_validate	-0.226522172032416	7.67246581249718e-22		NA	NA	NA
hsa-miR-21-5p	PI15	-0.142759573450327	0.928848736959803	0.867562682353867	0.0769578951856609	mirMAP	-0.86771201768414	2.26388320401889e-19		NA	NA	NA
hsa-miR-21-5p	PIK3C2A	-0.142759573450327	0.928848736959803	-0.393621191182813	0.473596350423844	miRNAWalker2_validate	-0.270607830740225	2.15289219729295e-23		NA	NA	NA
hsa-miR-21-5p	PIK3R1	-0.142759573450327	0.928848736959803	-0.241875449560569	0.714371520891375	miRNAWalker2_validate;MirTarget;miRNATAP	-0.442813578327247	2.68375696066295e-30	26676464	PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer	cell migration	breast cancer
hsa-miR-21-5p	PIKFYVE	-0.142759573450327	0.928848736959803	-0.058136521013485	0.901422997938988	MirTarget	-0.300600782379684	1.16284328419149e-30		NA	NA	NA
hsa-miR-21-5p	PJA2	-0.142759573450327	0.928848736959803	-0.738289669355749	0.254585720731366	MirTarget	-0.328671664212152	6.68261606142016e-39		NA	NA	NA
hsa-miR-21-5p	PKD2	-0.142759573450327	0.928848736959803	-0.0921691984173378	0.841376083834512	miRNAWalker2_validate	-0.32797302079002	2.50351907689772e-22		NA	NA	NA
hsa-miR-21-5p	PLAG1	-0.142759573450327	0.928848736959803	0.88136827405711	2.88716268845618e-06	MirTarget;miRNATAP	-0.297005735699583	1.97188045183731e-09		NA	NA	NA
hsa-miR-21-5p	PLAT	-0.142759573450327	0.928848736959803	-1.64917953060375	0.0102011346756403	miRNAWalker2_validate;miRTarBase	-0.15220333311024	0.00672105587093083	19308091	We also demonstrated that both deltaEF1 and TPA induced miR-21 expression		breast cancer
hsa-miR-21-5p	PLD1	-0.142759573450327	0.928848736959803	0.96914002327239	0.0104039344579489	miRNAWalker2_validate	-0.281512820182814	9.16457525889775e-13		NA	NA	NA
hsa-miR-21-5p	PLEKHA1	-0.142759573450327	0.928848736959803	0.257610971620987	0.587361454452888	miRNAWalker2_validate;MirTarget;miRNATAP	-0.107933087486065	1.70573247872038e-07		NA	NA	NA
hsa-miR-21-5p	PM20D2	-0.142759573450327	0.928848736959803	2.31204585097452	5.34783685289708e-10	miRNAWalker2_validate;MirTarget	-0.490778104606543	8.24217953453834e-19		NA	NA	NA
hsa-miR-21-5p	POLR3B	-0.142759573450327	0.928848736959803	0.0550884412847115	0.872946169764423	miRNAWalker2_validate	-0.117968642623395	9.63198896722134e-11		NA	NA	NA
hsa-miR-21-5p	PPARA	-0.142759573450327	0.928848736959803	1.34849061016039	0.00052894738945087	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.499807555240057	2.55196074428291e-42	22244963	In the final integrative analysis of lung cancer related miR-21-targets analysis 24 hub genes were identified by overlap calculation suggesting that miR-21 may play an important role in the development and progression of lung cancer through JAK/STAT signal pathway MAPK signaling pathway Wnt signaling pathway cell cycle PPAR signaling pathway apoptosis pathway and other pathways	progression	lung cancer
hsa-miR-21-5p	PPM1L	-0.142759573450327	0.928848736959803	0.565067157927387	0.00642492479705295	miRNAWalker2_validate	-0.487717639137822	9.57244035693759e-30		NA	NA	NA
hsa-miR-21-5p	PPP1R16B	-0.142759573450327	0.928848736959803	0.455785112739644	0.230109367001973	mirMAP	-0.493117259284243	2.21457165700942e-20		NA	NA	NA
hsa-miR-21-5p	PPP1R3B	-0.142759573450327	0.928848736959803	0.00952451967050205	0.986545360657392	MirTarget;miRNATAP	-0.256215158731733	1.69463202861492e-16		NA	NA	NA
hsa-miR-21-5p	PPP3CA	-0.142759573450327	0.928848736959803	-0.045650396271899	0.933852208885986	miRNATAP	-0.171857455775929	3.70280677648987e-09		NA	NA	NA
hsa-miR-21-5p	PREPL	-0.142759573450327	0.928848736959803	-0.418080370171887	0.463337254971082	miRNAWalker2_validate	-0.216844720365857	9.91267695171153e-23		NA	NA	NA
hsa-miR-21-5p	PRICKLE2	-0.142759573450327	0.928848736959803	-1.20320410751354	0.0117589308024567	miRNAWalker2_validate;MirTarget	-0.342742077988917	1.89622326605409e-18		NA	NA	NA
hsa-miR-21-5p	PRKAB2	-0.142759573450327	0.928848736959803	0.287513657933917	0.517478494035503	miRNAWalker2_validate	-0.110118016164516	3.47489548105212e-06		NA	NA	NA
hsa-miR-21-5p	PRKAR2A	-0.142759573450327	0.928848736959803	-0.30864568984061	0.382636256624685	mirMAP	-0.342627756886755	1.81049457969324e-14		NA	NA	NA
hsa-miR-21-5p	PRKCE	-0.142759573450327	0.928848736959803	-0.16567526720686	0.625602061444307	miRNAWalker2_validate	-0.277567939130377	4.73115836338224e-19		NA	NA	NA
hsa-miR-21-5p	PRKG1	-0.142759573450327	0.928848736959803	-1.23632701593604	8.33249873096374e-07	mirMAP	-0.291412972335893	6.26831369404863e-06		NA	NA	NA
hsa-miR-21-5p	PRRG1	-0.142759573450327	0.928848736959803	0.926317957633497	0.00349777131759485	MirTarget	-0.320684464127628	5.47382623869741e-16		NA	NA	NA
hsa-miR-21-5p	PTAR1	-0.142759573450327	0.928848736959803	0.231228968839103	0.641640838763669	miRNAWalker2_validate;mirMAP	-0.314881803765506	2.43582463683508e-33		NA	NA	NA
hsa-miR-21-5p	PTCH1	-0.142759573450327	0.928848736959803	1.19072004761017	0.000201104103918928	mirMAP	-0.521741532586734	2.36520849124019e-28		NA	NA	NA
hsa-miR-21-5p	PTCHD1	-0.142759573450327	0.928848736959803	3.52738952580155	1.82473230244176e-21	mirMAP	-0.766536202392272	2.72475491242887e-14		NA	NA	NA
hsa-miR-21-5p	PTEN	-0.142759573450327	0.928848736959803	-0.679266578822795	0.257263534525925	miRNAWalker2_validate;miRTarBase;mirMAP	-0.241962926381789	5.21884080644212e-25	26387181;21471222;26905520;25027758;21820606;24930006;22547075;26666820;23951172;25647415;22678116;22832383;19212625;22267008;26384051;22322462;27644439;21468550;20113523;21806946;24154840;27611950;24780321;23036707;23684551;26559642;26731559;26847601;27350731;24331411;25909227;24293118;26289851;24324076;23201752;17681183;25963606;27188433;22958183;22956424;19730150;21842656;21104017;23894315;23548551;26236156;24460329;20092645;25973032;25563770;24659669;26230405;25543482;20048743;27725205;23466500;22922228;25058005;20223231;27220494;24763002;24221338;22120473;21408027;23174819;22978663;25799148;26741162;23226804;26787105;26864640;26311740;25603978;26975392	In addition treated with 5-AZA resulted in significant increases of miR-21 expression in both MCF-7 and MDA-MB-231 cells P < 0.01 with the protein level of PTEN increased in MCF-7 cell which was further involved in the downregulation of AKT;Here we demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474 SKBR3 and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody whereas protein expression of the PTEN gene a miR-21 target was reduced; Rescuing PTEN expression with a p3XFLAG-PTEN-mut construct with deleted miR-21 targeting sequence at its 3' UTR restored the growth inhibition of trastuzumab in the resistant cells by inducing PTEN activation and AKT inhibition; In vivo administering miR-21 antisense oligonucleotides restored trastuzumab sensitivity in the resistant breast cancer xenografts by inducing PTEN expression whereas injection of miR-21 mimics conferred trastuzumab resistant in the sensitive breast tumors via PTEN silence;The effect of ZA on miR-21 expression was quantified by qRT-PCR and the amount of PTEN protein and its targets were analyzed by Western blot;In the same patients we also compared miR-21 expression with the expression of its presumed target PTEN; miR-21 expression levels were found to significantly correlate with tumour size r = 0.403 p = 0.009; Spearman's rank whereas no relation was found between miR-21 and PTEN expression levels Kruskal-Wallis test;MicroRNA 21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN; TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-21 and tumor suppressor gene PTEN protein; We showed that upregulation of miR-21 in MCF-7/ADR cells was concurrent with downregulation of PTEN protein; Overexpression of PTEN could mimic the same effects of miR-21 inhibitor in MCF-7/ADR cells and PTEN-siRNA could increase the resistance of MCF-7 cells to ADR; MiR-21 inhibitor could increase PTEN protein expression and the luciferase activity of a PTEN 3' untranslated region-based reporter construct in MCF-7/ADR cells; Dysregulation of miR-21 plays critical roles in the ADR resistance of breast cancer at least in part via targeting PTEN;In present study we determined the miR-21 levels in TNBC specimens and TNBC cell levels in vitro and then identified the role of miR-21 on tumor cell proliferation apoptosis and then identified PTEN as the possible target of the microRNA;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;PTEN a direct target gene of miR-21 was significantly downregulated in gemcitabine-resistant breast cancer cells and restoration of PTEN expression blocked miR-21-induced EMT and gemcitabine resistance;The results showed that knockdown of miR-21 by antagomir-21 decreased cell proliferation and induced apoptosis via targeting PTEN both in 4T1 cells and HUVECs;MiR-21 upregulation contributes to PTEN downregulation which is beneficial for the activation of PI3K/AKT signaling;Furthermore miR-21-induced upregulation of CSF-1 mRNA and its transcription were prevented by expression of PTEN mRNA lacking 3'-untranslated region UTR and miR-21 recognition sequence; Our results reveal a novel mechanism for the therapeutic function of fish oil diet that blocks miR-21 thereby increasing PTEN levels to prevent expression of CSF-1 in breast cancer;After miR-21 was transfected in MCF-7 cells PTEN protein level was measured by Western blot; Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt resulting in accumulation of Bad p21/WAF1/CIP1 and p27/KIP1;To further determine the potential involvement of miR-21 in breast cancer we have evaluated the expression level of miR-21 by stem-loop real-time RT-PCR based on SYBR-Green I in human invasive ductal carcinoma of the breast and we have correlated the results with clinicopathologic features and PTEN protein expression; The expression levels of miR-21 were correlated with PTEN and commonly used clinicopathologic features of breast cancer; Expression of miR-21 was negatively correlated with expression of PTEN P=0.013; These findings suggest that PTEN is possibly one of the targets of miR-21 in breast cancer and high expression of mir-21 indicates a more aggressive phenotype;microRNA 21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN; Thus in this study we focused on the expression and significance of miR-21 in gastric cancer tissues and the role of miR-21 in the biological behaviour and the expression of PTEN in gastric cancer cells; Western blotting and the Luciferase Reporter Assay were used to evaluate the change of PTEN expression after lowered expression of miR-21 in gastric cancer cell lines; The western blot results and Luciferase Reporter Assay demonstrated that PTEN expression was remarkably increased after miR-21 inhibition P<0.05 microRNA-21 expression was upregulated in gastric carcinoma tissues and was significantly associated with the degree of differentiation of tumour tissues local invasion and lymph node metastasis;Furthermore we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog PTEN the result of which can be rescued by miR-21 inhibitor;Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon;Bmi-1 also regulates p53 and PTEN via miR-21;The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting respectively in tumor tissues as well as adjacent non-tumor mucosa; miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues; A negative correlation between expression of miR-21 and PTEN was established in vivo;MicroRNA 21 inhibitor sensitizes human glioblastoma cells U251 PTEN mutant and LN229 PTEN wild type to taxol; Human glioblastoma U251 PTEN-mutant and LN229 PTEN wild-type cells were treated with taxol and the miR-21 inhibitor in a poly amidoamine PAMAM dendrimer alone or in combination; Interestingly the above data suggested that in both the PTEN mutant and the wild-type GBM cells miR-21 blockage increased the chemosensitivity to taxol; Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;NTR1 activation stimulates expression of miR-21 and miR-155 in colonocytes via Akt and NF-κB to down-regulate PTEN and SOCS1 and promote growth of tumors in mice;Correlations between the expression levels of miR-21 PTEN and p-AKT were analyzed by real-time PCR and Western blot test in HER2-positive GC cell lines; Overexpression of miR-21 down-regulated PTEN expression increased AKT phosphorylation and did not affect HER2 expression; Inversely suppression of miR-21 increased PTEN expression and down-regulated AKT phosphorylation but still did not affect HER2 expression;MicroRNA 21 promotes TGF β1 induced epithelial mesenchymal transition in gastric cancer through up regulating PTEN expression; In GC tissues the expressions of miR-21 Akt and p-Akt were up-regulated while PTEN expression was down-regulated; These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression;Expressions of PTEN were significantly down-regulated in CRC tissues and negatively correlated with expressions of Notch-1 r2=0.5207 p<0.01 and miR-21 r2=0.6996 p<0.01; These data indicate that the crosstalk between Notch-1 and miR-21 is involved in CRC development through degradation of PTEN;Over-expression of miR-21 suppressed its target PTEN and disrupted acinar morphogenesis;MicroRNA 21 suppresses PTEN and hSulf 1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways;There was overexpression of the miR-21 target genes PTEN by 67% and caspase-3 by 15% upon cotreatment;We previously reported that microRNA-21 miR-21 was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target PTEN; Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN stem-loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status; miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression; These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus;Triptolide reduces proliferation and enhances apoptosis of human non small cell lung cancer cells through PTEN by targeting miR 21; To the best of our knowledge the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21;Quantitative real-time PCR qRT-PCR and Western blot were used to detect the expression levels of miR-21 and PTEN in HCT116 HT29 Colo32 and SW480 CRC cell lines; Also the expression levels of PTEN mRNA and its downstream proteins AKT and PI3K in HCT116 cells after downregulating miR-21 were investigated; In comparing the levels of PTEN protein and downstream AKT and PI3K in HCT116 cells after downregulation of miR-21 expression the levels of AKT and PI3K protein expression significantly decreased P < 0.05; PTEN is one of the direct target genes of miR-21;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;MiR-21 level was inversely correlated with the levels of FOXO1 and PTEN in DLBCL cell lines; MiR-21 also down-regulated PTEN expression and consequently activated the PI3K/AKT/mTOR pathway which further decreased FOXO1 expression;MiR 21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non small cell lung cancer A549 cells; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA was performed to modulate the expression of microRNA-21 and PTEN under the treatment of curcumin; Moreover the protein level of PTEN a putative target of microRNA-21 was significantly elevated in curcumin-treated A549 cells as determined by Western blot analysis; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA significantly P < 0.05 reversed the growth suppression and apoptosis induction by curcumin compared to corresponding controls; Our data suggest a novel molecular mechanism in which inhibition of microRNA-21 and upregulation of PTEN mediate the anticancer activities of curcumin in NSCLC cells;PTEN gene expression was performed as a known target of miR-21;We also found that Rawq01 up-regulated the expression of PTEN through mir-21 inhibition and therefore inhibited the PI3K-AKT pathway;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;MicroRNA 21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer; PTEN was shown to be a direct target of miR-21 and to contribute to miR-21 effects on cell invasion; Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9 both downstream mediators of PTEN involved in cell migration and invasion; Aberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth migration and invasion;Overexpression of miR 21 promotes the proliferation and migration of cervical cancer cells via the inhibition of PTEN; The aim of this study was to examine the expression of miR-21 and PTEN in cervical cancer specimens using quantitative PCR; miR-21 was upregulated in the cervical cancer specimens negatively correlating with the PTEN mRNA level; Transfection of the miR-21 mimics was markedly promoted whereas the miR-21 inhibitor suppressed the proliferation migration and invasion of cervical cancer cells with a significant inhibition of PTEN expression; The present study showed the upregulation of miR-21 in invasive cervical cancers and confirmed the promotion of miR-21 with regard to the proliferation migration and invasion in cervical cancer cells via inhibiting the PTEN expression;The expressions of miR-21 PTEN PI3K and AKT were detected in 89 esophageal cancer samples and 58 adjacent normal tissues respectively; MiR-21 PI3K and AKT have higher expressions but PTEN has lower expression in esophageal cancer tissues compared with adjacent normal tissues; Further PTEN was a target gene of miR-21;The expression level of miR-21 and PTEN messenger RNA were measured by quantitative real-time reverse transcription polymerase chain reaction or reverse transcription polymerase chain reaction; miR-21 was overexpressed and PTEN was suppressed in established radioresistant TE-R60 cells compared with the parent cells 1.3-fold and 70.83%; The inhibition of miR-21 significantly increased the cells' radiosensitivity P < 0.05 and the PTEN protein expression 2.3-fold in TE-1 cells; Knockdown of PTEN in anti-miR-21 TE-1 cells could abrogate the miR-21 inhibition-induced radiosensitization P < 0.05; Inhibition of miR-21 increased radiosensitivity of esophageal cancer TE-1 cells and this effect was possibly through the activation of PTEN;MicroRNA 21 miR 21 expression promotes growth metastasis and chemo or radioresistance in non small cell lung cancer cells by targeting PTEN; Taken together these results provide evidence to show the promotion role of miR-21 in NSCLC development through modulation of the PTEN signaling pathway;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Role of microRNA 21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma; To evaluate the role of miR-21 and PTEN cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells; In Eca109 when transfected with miR-21 mimics accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40% which resulted in the promotion of cell proliferation; However when transfected with miR-21 inhibitor expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79% which resulted in the suppression of cell proliferation; Furthermore there was a significantly inverse correlation between miR-21 expression and PTEN protein levels p < 0.05; The author concluded that MiR-21 was overexpressed in vitro and ESCC and promoted the cell proliferation might target PTEN at post-transcriptional level and regulated the cancer invasion in Kazakh's ESCC;Difluorinated curcumin CDF restores PTEN expression in colon cancer cells by down regulating miR 21; Indeed our current data demonstrate a marked downregulation of PTEN in SCID mice xenografts of miR-21 over-expressing colon cancer HCT116 cells; Colonospheres that are highly enriched in cancer stem/stem like cells reveal increased miR-21 expression and decreased PTEN; Difluorinated curcumin CDF a novel analog of the dietary ingredient curcumin which has been shown to inhibit the growth of 5-Flurouracil + Oxaliplatin resistant colon cancer cells downregulated miR-21 in chemo-resistant colon cancer HCT116 and HT-29 cells and restored PTEN levels with subsequent reduction in Akt phosphorylation;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells;Finally invasion and metastasis assays were performed and alteration in mir-21 PTEN AKT and pAKT level was evaluated in these cells; Enhanced invasion and metastasis increased miR-21 expression decreased PTEN elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells;In the present study we investigated the role of miR-21 and its potential as a therapeutic target in two prostate cancer cell lines characterized by different miR-21 expression levels and PTEN gene status;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;Expressions of microRNA-21 miR-21 PTEN MMP9 and p47 were detected by qPCR;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;microRNA 21 Regulates Cell Proliferation and Migration and Cross Talk with PTEN and p53 in Bladder Cancer; MicroRNA-21 regulates proliferation and migration of bladder cancer cells and cross talk with PTEN and p53 in bladder cancer;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status; To explore whether miR-21 can serve as a therapeutic target for glioblastoma we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 PTEN mutant and LN229 PTEN wild-type GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo; Taken together our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated; In conclusion our data suggest that mUA can suppress cell viability in DU145 cells through modulating miR-21 and its downstream series-wound targets including PTEN Akt and Wnt/β-catenin signaling;miR 21 confers cisplatin resistance in gastric cancer cells by regulating PTEN; In addition miR-21 induced cell survival and cisplatin resistance through downregulating the expression of phosphatase and tension homolog deleted on chromosome 10 PTEN and activation of Akt pathway;Finally we demonstrate that modulation of tumor suppressors PTEN and p53 in U87 cells does not affect the decrease in miR-21 levels associated with PDGF-B overexpression;This study aimed to investigate whether NSCLC miR-21 mediated resistance to TKIs also results from Pten targeting; Here we show miR-21 promotes cancer by negatively regulating Pten expression in human NSCLC tissues: high miR-21 expression levels were associated with shorter DFS in 47 NSCLC patients; high miR-21/low Pten expression levels indicated a poor TKI clinical response and shorter overall survival in another 46 NSCLC patients undergoing TKI treatment; In vitro assays showed that miR-21 was up-regulated concomitantly to down-regulation of Pten in pc-9/GR cells in comparison with pc-9 cells; Moreover over-expression of miR-21 significantly decreased gefitinib sensitivity by down-regulating Pten expression and activating Akt and ERK pathways in pc-9 cells while miR-21 knockdown dramatically restored gefitinib sensitivity of pc-9/GR cells by up-regulation of Pten expression and inactivation of AKT and ERK pathways in vivo and in vitro;MicroRNA 21 miR 21 represses tumor suppressor PTEN and promotes growth and invasion in non small cell lung cancer NSCLC; We identified the role of miR-21 in non-small cell lung cancer NSCLC and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation; Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot respectively; Tumor tissues showed an inverse correlation between miR-21 and PTEN protein; miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines; miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC;MiR-21 overexpression decreases PTEN increases p-Akt and subsequently increases HIF-1α expression while miR-21 inhibition results in increased PTEN decreased p-Akt and then decreased HIF-1α;This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma DLBCL paraffin-embedded tissues and evaluate its potential relevance with clinical characteristics; In patients with DLBCL the expression level of miR-21 was negatively correlated with the level of PTEN protein; These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL;Down regulation of PTEN expression modulated by dysregulated miR 21 contributes to the progression of esophageal cancer; We demonstrated that knockdown of miR-21 significantly increased expression of PTEN protein; Our findings suggest that miR-21 could be a potential oncomiR probably by regulation of PTEN and a novel prognostic factor for ESCC patients;The levels of mir-21 did not associate with the expression of PTEN an important tumour suppressor in CRC and one of many putative targets of miR-21 but interestingly was associated with stage of disease in the PTEN expressing tumours;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants;The PTEN protein levels in CRC tissues and cells had an inverse correlation with miR-21 expression; MiR-21 targets PTEN at the post-transcriptional level and regulates cell proliferation and invasion in CRC;These findings demonstrate a novel role of AR in the regulation of miR-21 and its target PTEN in growth factor-induced colon cancer cell growth;Whether miR-21 regulated PTEN expression was assessed by luciferase assay; The RNA and protein levels of PTEN were significantly decreased by exogenous miR-21 and the 3'-untranslated region of PTEN was shown to be a target of miR-21;In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX;microRNA 21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer; We performed a qRT-PCR assay with miR-21 and PTEN in 16 paired EEC tumor tissues and adjacent non-tumor endometrium; To validate the putative binding site of miR-21 in the 3' untranslated region 3'-UTR of PTEN messenger RNA mRNA a dual-luciferase reporter assay was carried out; The upregulation of miR-21 led to a significant decrease in the PTEN protein expression level P=0.007; The downregulation of miR-21 led to a significant increase in PTEN protein P=0.002; In conclusion we demonstrated that the expression of PTEN protein but not mRNA was negatively directly regulated by miR-21 in the KLE cell line; The overexpression of miR-21 modulated EEC cell proliferation through the downregulation of PTEN;The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR 21 and promoter methylation; In this study we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC; PTEN mRNA level was negatively correlated with miR-21 level r = -0.595 P < 0.001; PTEN expression was also correlated directly with the PTEN mRNA level r = 0.583 P < 0.001 and conversely with miR-21 level r = -0.632 P < 0.001; This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;Exposure of HCC cells to sorafenib led to an increase in miR-21 expression a decrease in PTEN expression and sequential Akt activation;MicroRNA 21 controls hTERT via PTEN in human colorectal cancer cell proliferation; The aim of this study was to determine a role of microRNA-21 miRNA-21 in colorectal cancer CRC and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue PTEN;Relevance of miR 21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells; We identified the tumor suppressor gene PTEN as a target of miR-21 and determined the mechanism of its regulation throughout reporter construct plasmids; Using this model we analyzed the expression of miR-21 and PTEN as well as functional effects such as autophagy and apoptosis induction; In SiHa cells there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells; We conclude that miR-21 post-transcriptionally down-regulates the expression of PTEN to promote cell proliferation and cervical cancer cell survival	;drug resistance;;;drug resistance;;;drug resistance;;;;;;metastasis;differentiation;;tumorigenesis;;;;;;;;;progression;;;;;drug resistance;;;;;;cell migration;;;;metastasis;drug resistance;;;;;;;metastasis;;;;;;;progression;malignant trasformation;;drug resistance;poor survival;;drug resistance;poor survival;;;;progression;staging;;;;;;;;drug resistance;;;poor survival	breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;gastric cancer;cervical and endocervical cancer;colon cancer;gastric cancer;bladder cancer;glioblastoma;colon cancer;gastric cancer;gastric cancer;colorectal cancer;lung cancer;liver cancer;glioblastoma;melanoma;lung squamous cell cancer;colorectal cancer;lung squamous cell cancer;B cell lymphoma;lung squamous cell cancer;thyroid cancer;esophageal cancer;glioblastoma;liver cancer;cervical and endocervical cancer;esophageal cancer;esophageal cancer;lung squamous cell cancer;pancreatic cancer;lung cancer;esophageal cancer;colon cancer;B cell lymphoma;colon cancer;pancreatic cancer;prostate cancer;liver cancer;lung cancer;gastric cancer;bladder cancer;B cell lymphoma;glioblastoma;prostate cancer;gastric cancer;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;cervical and endocervical cancer;B cell lymphoma;esophageal cancer;colorectal cancer;pancreatic cancer;colorectal cancer;colon cancer;lung squamous cell cancer;lung cancer;endometrial cancer;colorectal cancer;pancreatic cancer;liver cancer;colorectal cancer;cervical and endocervical cancer
hsa-miR-21-5p	PTGFR	-0.142759573450327	0.928848736959803	1.40807000695688	1.93932589466733e-06	miRNAWalker2_validate	-0.744982504356861	1.57528731294261e-24		NA	NA	NA
hsa-miR-21-5p	PTPN14	-0.142759573450327	0.928848736959803	1.10022746640847	0.00148896142162313	miRNAWalker2_validate;MirTarget	-0.397227308894507	2.68785339242311e-19		NA	NA	NA
hsa-miR-21-5p	PTPRB	-0.142759573450327	0.928848736959803	-0.934024873846586	0.0490920158405168	mirMAP	-0.573556491022332	1.12847861657235e-41		NA	NA	NA
hsa-miR-21-5p	PTPRT	-0.142759573450327	0.928848736959803	-4.57678502148434	1.1836153605202e-17	mirMAP	-0.375910517469041	0.0012689006814031		NA	NA	NA
hsa-miR-21-5p	PTX3	-0.142759573450327	0.928848736959803	2.66507328303401	7.17417136277738e-17	miRNAWalker2_validate	-0.495263579161642	3.54088043602359e-09		NA	NA	NA
hsa-miR-21-5p	PURA	-0.142759573450327	0.928848736959803	-0.416493340975599	0.251172642764495	miRNAWalker2_validate	-0.155663617479665	1.10539908337268e-16		NA	NA	NA
hsa-miR-21-5p	PURB	-0.142759573450327	0.928848736959803	0.0133337541325975	0.982247831119202	miRNAWalker2_validate;mirMAP;miRNATAP	-0.120679210280138	6.04808886411543e-13		NA	NA	NA
hsa-miR-21-5p	PYGO1	-0.142759573450327	0.928848736959803	0.0718767766282624	0.779700537644378	mirMAP	-0.488388055538722	1.24796373463689e-15		NA	NA	NA
hsa-miR-21-5p	RAB11FIP2	-0.142759573450327	0.928848736959803	-0.195186300969823	0.62504337863968	miRNAWalker2_validate	-0.29824575737227	1.91429477956097e-43		NA	NA	NA
hsa-miR-21-5p	RAB30	-0.142759573450327	0.928848736959803	-1.70569527781604	1.18658635861988e-07	mirMAP	-0.264676085801825	2.7904893470383e-07		NA	NA	NA
hsa-miR-21-5p	RABGAP1	-0.142759573450327	0.928848736959803	-0.0795944389547811	0.888996873258736	miRNAWalker2_validate	-0.134129704547964	2.97349628454221e-16		NA	NA	NA
hsa-miR-21-5p	RAPGEF1	-0.142759573450327	0.928848736959803	0.215917912470729	0.718463555275633	mirMAP	-0.137769492193029	9.12627757448983e-16		NA	NA	NA
hsa-miR-21-5p	RAPGEF6	-0.142759573450327	0.928848736959803	-0.760588993982337	0.0319105067198291	miRNAWalker2_validate	-0.264839527208078	3.17267996573965e-07		NA	NA	NA
hsa-miR-21-5p	RAPH1	-0.142759573450327	0.928848736959803	-0.104718011721031	0.849843210074317	miRNAWalker2_validate;mirMAP	-0.118213062647856	1.56328984637089e-05		NA	NA	NA
hsa-miR-21-5p	RASA1	-0.142759573450327	0.928848736959803	-0.55530974978949	0.289503283187035	miRNATAP	-0.149767999849588	3.5184635352839e-08	25663768;27101583	RKO cells were transfected with vectors overexpressing or down-regulating either miR-21 or RASA1; Furthermore a luciferase reporter assay was used to determine whether RASA1 is a gene target of miR-21; RASA1 protein levels were significantly decreased in RKO cells compared with the other 5 colon cancer cell lines and RASA1 was confirmed as a target gene of miR-21; Interestingly RASA1 mRNA and protein levels in pre-miR-21-LV up-regulation of miR-21 cells were lower than those in anti-miR-21-LV down-regulation of miR-21 cells P < 0.05; RASA1 is a target gene of miR-21 which promotes malignant behaviors of RKO cells through regulation of RASA1 expression;Circulating MicroRNA 21 Is Involved in Lymph Node Metastasis in Cervical Cancer by Targeting RASA1; Then cervical cancer cell lines HeLa HPV-18 DNA E6/E7RNA and HT-3 HPV DNA E6/E7RNA were used to confirm the interaction between miR-21 and RASA1; MicroRNA-21 reduces RASA1 expression in cervical cancer cell lines and promotes cervical cancer cell migration via RASA1	malignant trasformation;metastasis;cell migration	colon cancer;cervical and endocervical cancer
hsa-miR-21-5p	RB1	-0.142759573450327	0.928848736959803	-0.748501609659556	0.146294348691753	miRNAWalker2_validate	-0.178426586251413	2.52614483801181e-10		NA	NA	NA
hsa-miR-21-5p	RBPJ	-0.142759573450327	0.928848736959803	0.107305888994804	0.812371656947911	MirTarget;miRNATAP	-0.18411640822153	6.25474791035248e-26		NA	NA	NA
hsa-miR-21-5p	RECK	-0.142759573450327	0.928848736959803	-0.220445932612625	0.58018148771276	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.34438163056236	1.21607205369305e-16	19730150;20480266;22642976;18794849;25720799	Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;Furthermore we identified that RECK reversion-inducing-cysteine-rich protein with kazal motifs a tumor suppressor gene was a direct target of miR-21; Our results suggest that miR-21 expression has a key role in regulating cellular processes in osteosarcoma likely through regulating RECK and may serve as a therapeutic target;miR 21 may acts as an oncomir by targeting RECK a matrix metalloproteinase regulator in prostate cancer; The micro RNA miRNA mir-21 has been shown to regulate invasiveness in cancer through translational repression of the Metaloproteinase MMP inhibitor RECK; Our aim is to investigate the levels of expression of RECK and miR-21 in PCa comparing with classical prognostic factors and disease outcome and also test if RECK is a target of miR-21 in in vitro study using PCa cell line; To determine if RECK is a target of miR-21 in prostate cancer we performed an in vitro assay with PCa cell line DU-145 transfected with pre-miR-21 and anti-miR-21; To determine miR-21 and RECK expression levels in PCa samples we performed quantitative real-time polymerase chain reaction qRT-PCR; From men who presented the second profile miR-21 overexpression and RECK underexpression 91.7% were staged pT3; Our results demonstrate RECK as a target of miR-21; We believe that miR-21 may be important in PCa progression through its regulation of RECK a known regulator of tumor cell invasion;Finally we showed that RECK a known tumor suppressor in gastric cancer is a bona fide target of miR-21; Taken together miR-21 may be important in the initiation and progression of gastric cancers as an oncomiR likely through regulating RECK; Our findings suggest a potential regulatory pathway in which H pylori infection upregulates expression of miR-21 which in turn downregulates RECK and then leads to the development of gastric cancer;We verified that HMGB1-induced expression of miR-21 in HCC provides a posttranscriptional repression of the matrix metalloproteinase MMP inhibitors RECK and TIMP3 which are known to impact HCC progression and metastases; Finally we found that inhibition of miR-21 in murine HMGB1-overexpressing HCC xenografts led to reduced tumor MMP activity through released repression of the miR-21 targets RECK and TIMP3 which ultimately impeded tumor progression	;;staging;progression;progression;metastasis;progression	pancreatic cancer;sarcoma;prostate cancer;gastric cancer;liver cancer
hsa-miR-21-5p	REST	-0.142759573450327	0.928848736959803	-0.272236135165405	0.442523339412442	miRNAWalker2_validate;miRTarBase;mirMAP	-0.234347286034385	4.181202706174e-06		NA	NA	NA
hsa-miR-21-5p	REV1	-0.142759573450327	0.928848736959803	0.0433295448376096	0.927311706990176	miRNAWalker2_validate	-0.169125772204719	2.51649067413995e-35		NA	NA	NA
hsa-miR-21-5p	REV3L	-0.142759573450327	0.928848736959803	0.215216303522914	0.637966552193029	miRNAWalker2_validate	-0.395951006339526	2.50387473559502e-35		NA	NA	NA
hsa-miR-21-5p	RHOQ	-0.142759573450327	0.928848736959803	0.66362027500911	0.255038793342011	miRNAWalker2_validate	-0.331156761026688	1.86377123477213e-35		NA	NA	NA
hsa-miR-21-5p	RMND5A	-0.142759573450327	0.928848736959803	0.182575469028045	0.754368677291852	miRNAWalker2_validate;MirTarget;miRNATAP	-0.230725919786101	4.5541830124258e-32		NA	NA	NA
hsa-miR-21-5p	RNF11	-0.142759573450327	0.928848736959803	-0.111453014827159	0.857727164919893	miRNAWalker2_validate	-0.207707905814412	1.86121810116098e-28		NA	NA	NA
hsa-miR-21-5p	RNF111	-0.142759573450327	0.928848736959803	-0.298310842108625	0.50684656902575	miRNATAP	-0.149067914864918	1.40190259258715e-12		NA	NA	NA
hsa-miR-21-5p	RNF182	-0.142759573450327	0.928848736959803	1.85646573000999	2.24192328912263e-12	MirTarget	-0.305573934243	6.92453690347356e-06		NA	NA	NA
hsa-miR-21-5p	ROR1	-0.142759573450327	0.928848736959803	0.870819396933328	0.000483935407562208	miRNATAP	-0.434393200893694	1.79063796146941e-11		NA	NA	NA
hsa-miR-21-5p	RPS6KA3	-0.142759573450327	0.928848736959803	0.774053316496799	0.120196415930518	miRNAWalker2_validate;mirMAP;miRNATAP	-0.318123624363205	1.65398865078165e-19		NA	NA	NA
hsa-miR-21-5p	RRAGC	-0.142759573450327	0.928848736959803	0.322016603139972	0.390158352609154	miRNAWalker2_validate	-0.260892436864086	4.61156448903526e-18		NA	NA	NA
hsa-miR-21-5p	RSBN1	-0.142759573450327	0.928848736959803	-0.16185844263064	0.714312007987598	miRNATAP	-0.210119054420049	3.66501148874656e-26		NA	NA	NA
hsa-miR-21-5p	RSF1	-0.142759573450327	0.928848736959803	-0.0129873349957785	0.978461279311136	miRNAWalker2_validate	-0.16893371246655	1.7636669179261e-08		NA	NA	NA
hsa-miR-21-5p	RTN4	-0.142759573450327	0.928848736959803	-0.111088211057259	0.885492053570861	miRNAWalker2_validate;miRTarBase	-0.190348578635845	5.32438743284111e-26		NA	NA	NA
hsa-miR-21-5p	SACM1L	-0.142759573450327	0.928848736959803	-0.256193903110328	0.617593521322788	miRNAWalker2_validate	-0.212024177398943	4.75997508608528e-34		NA	NA	NA
hsa-miR-21-5p	SAMD5	-0.142759573450327	0.928848736959803	0.133860523693607	0.677996351569049	miRNAWalker2_validate	-0.716846378058606	6.19437362584202e-28		NA	NA	NA
hsa-miR-21-5p	SAMD8	-0.142759573450327	0.928848736959803	-0.47175674268426	0.0524779372511359	mirMAP	-0.358979847151263	4.88149297609557e-28		NA	NA	NA
hsa-miR-21-5p	SAR1A	-0.142759573450327	0.928848736959803	-0.105067836505359	0.871811727863015	miRNAWalker2_validate	-0.100987634950651	1.50952128914346e-11		NA	NA	NA
hsa-miR-21-5p	SASH1	-0.142759573450327	0.928848736959803	-0.161475331603732	0.773555121664429	miRNAWalker2_validate;MirTarget	-0.459482061655079	1.48929633699321e-29		NA	NA	NA
hsa-miR-21-5p	SATB1	-0.142759573450327	0.928848736959803	0.544683251522467	0.200701844420834	MirTarget;miRNATAP	-0.367776335653766	2.454140715956e-17	27107063	SATB1 is Down regulated in Clear Cell Renal Cell Carcinoma and Correlates with miR 21 5p Overexpression and Poor Prognosis; This study aimed to determine the expression levels of SATB1 as well as miR-21-5p -the post-transcriptional repressor of SATB1 expression- in clear cell renal cell carcinoma ccRCC and to investigate their association with the progression of ccRCC; Immunohistochemistry and quantitative polymerase chain reaction were used to assess the expression of SATB1 protein and mRNA as well as miR-21-5p in tumor and matched normal kidney tissues collected from 56 ccRCC patients; SATB1 mRNA level was down-regulated in ccRCC tissue and inversely correlated with the content of miR-21-5p; Down-regulation of SATB1 mRNA and up-regulation of miR-21-5p were associated with shorter patient survival; Decreased expression of SATB1 in ccRCC may result from over-expressed miR-21-5p	worse prognosis;progression;poor survival	kidney renal cell cancer
hsa-miR-21-5p	SBNO1	-0.142759573450327	0.928848736959803	-0.184690362856148	0.650166354939554	mirMAP	-0.247201602083685	2.57673313176957e-07		NA	NA	NA
hsa-miR-21-5p	SCML2	-0.142759573450327	0.928848736959803	1.47765275190511	3.6624168519922e-12	MirTarget;miRNATAP	-0.181409503310777	0.000290066888741199		NA	NA	NA
hsa-miR-21-5p	SEC14L5	-0.142759573450327	0.928848736959803	0.208576607599555	0.391531345777183	mirMAP	-0.65488986326132	7.81502049229416e-29		NA	NA	NA
hsa-miR-21-5p	SEC22C	-0.142759573450327	0.928848736959803	-0.448708707138638	0.331820053248203	mirMAP	-0.137892439272061	8.62760683515707e-17		NA	NA	NA
hsa-miR-21-5p	SEC62	-0.142759573450327	0.928848736959803	-0.304824690359139	0.653245680259212	mirMAP	-0.172609777894466	5.03430786796368e-20		NA	NA	NA
hsa-miR-21-5p	SEMA5A	-0.142759573450327	0.928848736959803	-1.02473794225766	0.0355962725330142	miRNAWalker2_validate	-0.339179931026802	2.91371817319389e-12		NA	NA	NA
hsa-miR-21-5p	SERAC1	-0.142759573450327	0.928848736959803	0.374213238797429	0.246967520257469	miRNAWalker2_validate	-0.120613392995982	5.44233291790678e-08		NA	NA	NA
hsa-miR-21-5p	SERPINI1	-0.142759573450327	0.928848736959803	-1.25242115554011	0.000635492464903451	miRNAWalker2_validate	-0.267869656654958	1.79799152826543e-07	22464652	MicroRNA 21 inhibits Serpini1 a gene with novel tumour suppressive effects in gastric cancer; Molecular techniques include microRNA microarrays cDNA microarrays qRT-PCR for miR and mRNA expression transfection of MKN28 with miR-21 inhibitor or Serpini1 followed by Western blotting cell cycle analysis by flow cytometry and luciferase reporter assay; This search identified Serpini1 as a putative miR-21 target; Luciferase assays demonstrated direct interaction between miR-21 and Serpini1 3'UTR miR-21 and Serpini1 expression levels were inversely correlated in a subgroup of gastric cancers suggesting a regulatory mechanism that included both of these molecules; Taken together these data suggest that in a subgroup of gastric cancers miR-21 is upregulated inducing downregulation of Serpini1 which in turn releases the G1-S transition checkpoint with the end result being increased tumour growth		gastric cancer
hsa-miR-21-5p	SESN1	-0.142759573450327	0.928848736959803	-0.678910827390565	0.155008112067661	miRNAWalker2_validate	-0.28257542976425	2.54012028934313e-23		NA	NA	NA
hsa-miR-21-5p	SESTD1	-0.142759573450327	0.928848736959803	0.157872090426435	0.77857727425336	miRNAWalker2_validate;mirMAP	-0.305477983994508	1.39045415565243e-25		NA	NA	NA
hsa-miR-21-5p	SGCB	-0.142759573450327	0.928848736959803	0.102970476363384	0.839785890611535	miRNAWalker2_validate	-0.380361072104493	9.14013099981249e-40		NA	NA	NA
hsa-miR-21-5p	SGK3	-0.142759573450327	0.928848736959803	-0.373910002568633	0.456720297208525	miRNAWalker2_validate	-0.123722166946783	0.00594242169029888		NA	NA	NA
hsa-miR-21-5p	SGTB	-0.142759573450327	0.928848736959803	0.156989075499647	0.592591359438376	mirMAP	-0.144408981565387	3.14985679730937e-10		NA	NA	NA
hsa-miR-21-5p	SH3BGRL2	-0.142759573450327	0.928848736959803	-0.591940542334678	0.166680919817026	mirMAP	-0.589580081826542	4.07541127538102e-43		NA	NA	NA
hsa-miR-21-5p	SIM1	-0.142759573450327	0.928848736959803	1.19497851255751	0.0029659423913165	mirMAP	-0.750621803033214	3.71689711035348e-16		NA	NA	NA
hsa-miR-21-5p	SKI	-0.142759573450327	0.928848736959803	0.310734370883258	0.61201530608366	MirTarget;miRNATAP	-0.187847853952807	1.72284744033067e-22		NA	NA	NA
hsa-miR-21-5p	SKIV2L2	-0.142759573450327	0.928848736959803	-0.454385537550221	0.412190313284798	mirMAP	-0.115753722183293	1.05918585352273e-10		NA	NA	NA
hsa-miR-21-5p	SLAIN2	-0.142759573450327	0.928848736959803	-0.211865312999925	0.698053809680442	miRNAWalker2_validate	-0.155076505137744	7.73778851496149e-18		NA	NA	NA
hsa-miR-21-5p	SLC17A5	-0.142759573450327	0.928848736959803	0.227162350745649	0.62668809873714	miRNAWalker2_validate	-0.141717076512453	1.15572536843549e-09		NA	NA	NA
hsa-miR-21-5p	SLC26A2	-0.142759573450327	0.928848736959803	0.684790645009001	0.151137675444292	miRNAWalker2_validate;mirMAP	-0.230833931172102	1.65545587085468e-10		NA	NA	NA
hsa-miR-21-5p	SLC5A3	-0.142759573450327	0.928848736959803	0.154429091253622	0.745381446324516	miRNAWalker2_validate	-0.159656869345367	8.71109577015147e-07		NA	NA	NA
hsa-miR-21-5p	SLC7A6	-0.142759573450327	0.928848736959803	0.232885797549137	0.643812101941317	miRNATAP	-0.127162122697708	7.65678085510232e-11		NA	NA	NA
hsa-miR-21-5p	SLC9A6	-0.142759573450327	0.928848736959803	1.18033638416743	0.000744383662356913	miRNAWalker2_validate;miRNATAP	-0.27005054250097	2.15261298062036e-15		NA	NA	NA
hsa-miR-21-5p	SLFN5	-0.142759573450327	0.928848736959803	0.374385696163092	0.402323380678066	mirMAP	-0.270331943733987	4.96688585043486e-09		NA	NA	NA
hsa-miR-21-5p	SLK	-0.142759573450327	0.928848736959803	-0.227384936542899	0.695387429917494	miRNAWalker2_validate	-0.209515781521834	1.03916806668674e-18		NA	NA	NA
hsa-miR-21-5p	SLMAP	-0.142759573450327	0.928848736959803	-0.318553603760041	0.55107092598671	miRNAWalker2_validate;MirTarget	-0.194986340943015	2.01853738483389e-20		NA	NA	NA
hsa-miR-21-5p	SNED1	-0.142759573450327	0.928848736959803	-1.1948779970144	0.00962183847217995	miRNATAP	-0.154303618359808	3.36056645429983e-05		NA	NA	NA
hsa-miR-21-5p	SNRK	-0.142759573450327	0.928848736959803	-0.27968974642189	0.588917916663615	miRNAWalker2_validate	-0.28713135980416	8.46878458998811e-43		NA	NA	NA
hsa-miR-21-5p	SNRNP48	-0.142759573450327	0.928848736959803	-0.0533008763925364	0.890220883403793	miRNAWalker2_validate	-0.12263221492908	2.95778121000395e-11		NA	NA	NA
hsa-miR-21-5p	SNX1	-0.142759573450327	0.928848736959803	-0.488635648983442	0.458982832622621	mirMAP	-0.238721111526076	1.72689774002533e-35		NA	NA	NA
hsa-miR-21-5p	SNX29	-0.142759573450327	0.928848736959803	-0.346646990011627	0.453477572034779	miRNATAP	-0.137993773168834	5.43402751369132e-11		NA	NA	NA
hsa-miR-21-5p	SNX30	-0.142759573450327	0.928848736959803	-0.308431905966599	0.50084369048469	miRNAWalker2_validate	-0.103936225222875	5.88654906513536e-06		NA	NA	NA
hsa-miR-21-5p	SOCS5	-0.142759573450327	0.928848736959803	0.415754010623421	0.382406483832321	miRNAWalker2_validate	-0.252298585835556	7.63709510228412e-31		NA	NA	NA
hsa-miR-21-5p	SOD3	-0.142759573450327	0.928848736959803	0.924947965524093	0.0422578906926585	miRNAWalker2_validate;miRTarBase	-0.541158204506034	1.67623518096689e-19		NA	NA	NA
hsa-miR-21-5p	SOS2	-0.142759573450327	0.928848736959803	-0.0819221582890739	0.875016091339336	miRNATAP	-0.267389788495656	7.45996997519246e-36		NA	NA	NA
hsa-miR-21-5p	SOX5	-0.142759573450327	0.928848736959803	-0.638169457802572	0.0128943060778149	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.756030812927406	1.40346547972103e-30		NA	NA	NA
hsa-miR-21-5p	SOX6	-0.142759573450327	0.928848736959803	2.32345688667324	1.18049017532599e-16	MirTarget;miRNATAP	-0.614523344099219	1.85455711268023e-17		NA	NA	NA
hsa-miR-21-5p	SOX7	-0.142759573450327	0.928848736959803	0.168479475881452	0.590343824166888	miRNATAP	-0.469582240491516	1.09791134352581e-30		NA	NA	NA
hsa-miR-21-5p	SP1	-0.142759573450327	0.928848736959803	-0.343848657354394	0.599692956229942	miRNAWalker2_validate;miRTarBase	-0.155768447861795	3.88544448292272e-22		NA	NA	NA
hsa-miR-21-5p	SPATA18	-0.142759573450327	0.928848736959803	-1.12014132745382	0.00174018259980277	mirMAP	-0.509548577055583	1.87759596675863e-19		NA	NA	NA
hsa-miR-21-5p	SPG11	-0.142759573450327	0.928848736959803	-0.784522045540831	0.175414196347813	miRNAWalker2_validate	-0.146560576949795	9.20747133839781e-10		NA	NA	NA
hsa-miR-21-5p	SPG20	-0.142759573450327	0.928848736959803	0.230328628565122	0.661715764806857	miRNATAP	-0.373777345855725	8.83147806164101e-40		NA	NA	NA
hsa-miR-21-5p	SPRY1	-0.142759573450327	0.928848736959803	-0.537748045323871	0.291345781963126	miRNATAP	-0.446564917887034	6.78372597097293e-27	24155920	Functional studies showed that miR-21 over-expression in GICs induced comparable cell differentiation features and targeted SPRY1 mRNA which encodes for a negative regulator of neural stem-cell differentiation	differentiation	glioblastoma
hsa-miR-21-5p	SPRY2	-0.142759573450327	0.928848736959803	0.739192301049848	0.0660728853893468	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.684722889689909	7.12948810098069e-54	22322462	Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon	tumorigenesis	colon cancer
hsa-miR-21-5p	SPTLC3	-0.142759573450327	0.928848736959803	-0.00840645587132416	0.980981195478222	miRNAWalker2_validate	-0.14825479670126	0.000620543662309423		NA	NA	NA
hsa-miR-21-5p	SSFA2	-0.142759573450327	0.928848736959803	-0.120673689520749	0.847094944714844	miRNAWalker2_validate;miRNATAP	-0.297126225518918	3.37386167687646e-13		NA	NA	NA
hsa-miR-21-5p	STAG2	-0.142759573450327	0.928848736959803	0.047425665405882	0.939405201882075	miRNAWalker2_validate;MirTarget;miRNATAP	-0.238585426475483	1.76104366677348e-30		NA	NA	NA
hsa-miR-21-5p	STK40	-0.142759573450327	0.928848736959803	0.472732896799581	0.403044654366652	MirTarget;miRNATAP	-0.124884333810775	7.46670904029131e-11		NA	NA	NA
hsa-miR-21-5p	STXBP5	-0.142759573450327	0.928848736959803	-0.0120257172560372	0.97514185784775	miRNAWalker2_validate	-0.166193373708881	4.68683052604661e-08		NA	NA	NA
hsa-miR-21-5p	SV2B	-0.142759573450327	0.928848736959803	0.423835454648333	0.0939049198016824	mirMAP	-0.63070002075403	1.78985966619396e-24		NA	NA	NA
hsa-miR-21-5p	SYNE2	-0.142759573450327	0.928848736959803	0.132733426728924	0.838237637680498	miRNAWalker2_validate	-0.314978762049673	4.46077566676347e-27		NA	NA	NA
hsa-miR-21-5p	SYNRG	-0.142759573450327	0.928848736959803	-0.287432469205567	0.580247170353912	mirMAP	-0.128433486101485	2.72109305887873e-10		NA	NA	NA
hsa-miR-21-5p	SYT15	-0.142759573450327	0.928848736959803	0.412298480523128	0.110892603988312	MirTarget	-0.475462414278198	3.39520711442358e-21		NA	NA	NA
hsa-miR-21-5p	TAF1	-0.142759573450327	0.928848736959803	-0.229028569906709	0.661893434428497	miRNAWalker2_validate	-0.198143508696272	2.11873995485272e-18		NA	NA	NA
hsa-miR-21-5p	TET1	-0.142759573450327	0.928848736959803	0.971914499890692	4.56281446355525e-07	miRNAWalker2_validate	-0.219366694016521	3.08336716646783e-09		NA	NA	NA
hsa-miR-21-5p	TFCP2L1	-0.142759573450327	0.928848736959803	2.02107571489613	3.31299864743463e-05	mirMAP	-0.240788917483754	0.00101509498232268		NA	NA	NA
hsa-miR-21-5p	TGFBR2	-0.142759573450327	0.928848736959803	-0.246514824067334	0.7050497614235	miRNAWalker2_validate;miRTarBase;miRNATAP	-0.537594809270263	3.47307989185754e-40	24037531	Androgen receptor and microRNA 21 axis downregulates transforming growth factor beta receptor II TGFBR2 expression in prostate cancer; Our results revealed that miR-21 suppresses TGFBR2 levels by binding to its 3'-UTR and AR signaling further potentiates this effect in both untransformed and transformed human prostate epithelial cells as well as in human prostate cancers; Manipulation of androgen signaling or the expression levels of AR or miR-21 negatively altered TGFBR2 expression in untransformed and transformed human prostate epithelial cells human prostate cancer xenografts and mouse prostate glands; Together these results suggest that the AR and miR-21 axis exerts its oncogenic effects in prostate tumors by downregulating TGFBR2 hence inhibiting the tumor-suppressive activity of TGFβ pathway		prostate cancer
hsa-miR-21-5p	TGFBR3	-0.142759573450327	0.928848736959803	-0.250325459308409	0.65335981637459	miRNAWalker2_validate	-0.991051228961082	2.79000222280721e-56		NA	NA	NA
hsa-miR-21-5p	THRB	-0.142759573450327	0.928848736959803	-1.20141153466674	0.00835555391894914	MirTarget;miRNATAP	-0.535675702705463	3.68641251340418e-32	21159845	Direct interaction with THRB was shown for miR-21 and miR-146a; We observed lower levels of THRB transcripts in cell lines transfected with miR-21 -146a and -221 down-regulation of 37-48%; P < 0.0001 but not with miR-181a; The analysis of tumor/normal tissue pairs revealed down-regulation of THRB in 11 of 13 pairs 1.3- to 9.1-fold and up-regulation of miR-21 -146a -181a and -221 in almost all pairs		thyroid cancer
hsa-miR-21-5p	TIMP3	-0.142759573450327	0.928848736959803	-1.14468015317392	0.18426641286494	miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP	-0.194696613681374	9.95965481820086e-06	23936642;20346171;21820586;25720799;25587717	Genetic heterogeneity of breast cancer metastasis may be related to miR 21 regulation of TIMP 3 in translation; The aim of this study is to determine whether microRNA-21 miR-21 a specific microRNA implicated in multiple aspects of carcinogenesis promoted breast cancer metastasis by regulating the tissue inhibitor of metalloproteinase 3 TIMP-3 gene; Correlation analysis was performed between miR-21 and TIMP-3; There was significantly inverse correlation between miR-21 and TIMP-3 extracted from tissue;The aim of this study was to determine whether microRNA-21 miR-21 a specific microRNA implicated in multiple aspects of carcinogenesis impacts breast cancer invasion by regulating the tissue inhibitor of metalloproteinase 3 TIMP3 gene; In addition the regulation of tissue inhibitor of metalloproteinase 3 TIMP3 by miR-21 was evaluated by western blotting and luciferase assays; Evaluation of TIMP3 protein levels a peptidase involved in extarcellular matrix degredation inversely correlated with miR-21 expression; As knockdown of miR-21 increased TIMP3 protein expression and luciferase reporter activity our data suggests that miR-21 could promote invasion in breast cancer cells via its regulation of TIMP3;The effect of TIMP3 on miR-21-induced cell survival was determined by transfection with TIMP3 lacking 3' untranslated region and miR-21;We verified that HMGB1-induced expression of miR-21 in HCC provides a posttranscriptional repression of the matrix metalloproteinase MMP inhibitors RECK and TIMP3 which are known to impact HCC progression and metastases; Finally we found that inhibition of miR-21 in murine HMGB1-overexpressing HCC xenografts led to reduced tumor MMP activity through released repression of the miR-21 targets RECK and TIMP3 which ultimately impeded tumor progression;It was previously shown by our group that miR-21 a potential regulator of TIMP3 is over-expressed in cutaneous melanoma; It was therefore hypothesized that increased levels of miR-21 expression would lead to decreased expression of TIMP3 and thereby enhance the invasiveness of melanoma cells; Reduced expression of TIMP3 was achieved by siRNA knockdown and significantly enhanced invasion of melanoma cell lines mimicking the effects of miR-21 over-expression; Treatment of tumor cells with a linked nucleic acid antagomir to miR-21 inhibited tumor growth and increased tumor expression of TIMP3 in vivo in 01B74 Athymic NCr-nu/nu mice; This data shows that increased expression of miR-21 enhanced the invasive potential of melanoma cell lines through TIMP3 inhibition	metastasis;tumorigenesis;tumorigenesis;poor survival;metastasis;progression;	breast cancer;breast cancer;kidney renal cell cancer;liver cancer;melanoma
hsa-miR-21-5p	TLR4	-0.142759573450327	0.928848736959803	-0.0657270911701575	0.874054696214638	miRNAWalker2_validate	-0.359793062620959	1.10927793812338e-22	27286259	For in vivo relevance expression of TLR4 was correlated with miR-21 expression and ROS production in freshly isolated untreated primary human lung cancer cells		lung cancer
hsa-miR-21-5p	TMEM170B	-0.142759573450327	0.928848736959803	-0.274501004962679	0.391438341757926	mirMAP	-0.509535208739174	2.47092730947633e-51		NA	NA	NA
hsa-miR-21-5p	TMEM56	-0.142759573450327	0.928848736959803	0.404172433143196	0.256253208110967	miRNAWalker2_validate;mirMAP	-0.421346454804842	3.02279529827395e-17		NA	NA	NA
hsa-miR-21-5p	TMX4	-0.142759573450327	0.928848736959803	0.493179797495776	0.324955735413161	miRNAWalker2_validate;mirMAP	-0.152867366054518	3.08441420379405e-08		NA	NA	NA
hsa-miR-21-5p	TNFRSF11B	-0.142759573450327	0.928848736959803	-0.0243805723500006	0.931619832694438	miRNAWalker2_validate	-0.242363243955373	0.000195848073067522		NA	NA	NA
hsa-miR-21-5p	TNPO1	-0.142759573450327	0.928848736959803	-0.241966638310553	0.698888330802217	miRNAWalker2_validate;mirMAP	-0.101894159495048	2.4075932327283e-07		NA	NA	NA
hsa-miR-21-5p	TNRC6B	-0.142759573450327	0.928848736959803	0.22302137183309	0.639187433629322	miRNAWalker2_validate;miRNATAP	-0.221568273286781	1.07896083121108e-25		NA	NA	NA
hsa-miR-21-5p	TOPORS	-0.142759573450327	0.928848736959803	0.0609007245957853	0.891081923111771	miRNAWalker2_validate	-0.213687255984307	6.8590292232134e-30		NA	NA	NA
hsa-miR-21-5p	TOR1AIP2	-0.142759573450327	0.928848736959803	0.0640101441062102	0.867179336837162	mirMAP	-0.102442673178048	0.00173123353353528		NA	NA	NA
hsa-miR-21-5p	TP63	-0.142759573450327	0.928848736959803	-0.751479739219152	0.0903423487332178	miRNAWalker2_validate	-0.557133918156164	4.86451790960546e-10		NA	NA	NA
hsa-miR-21-5p	TPRG1L	-0.142759573450327	0.928848736959803	-0.0306370193959333	0.957282300031356	miRNAWalker2_validate;MirTarget	-0.18209864158624	1.51513313134975e-25		NA	NA	NA
hsa-miR-21-5p	TRIM2	-0.142759573450327	0.928848736959803	1.76226882161348	0.000372259364985161	miRNAWalker2_validate;mirMAP;miRNATAP	-0.506416520204349	1.77271267675961e-24		NA	NA	NA
hsa-miR-21-5p	TRIM33	-0.142759573450327	0.928848736959803	0.0559261003272846	0.917026479704382	miRNAWalker2_validate;miRNATAP	-0.119721587344264	1.98149738948897e-08		NA	NA	NA
hsa-miR-21-5p	TRPM7	-0.142759573450327	0.928848736959803	-0.260053697635848	0.606303188865266	miRNAWalker2_validate;miRNATAP	-0.199895215552816	1.13560815427112e-18		NA	NA	NA
hsa-miR-21-5p	TSHZ1	-0.142759573450327	0.928848736959803	-0.596412252441346	0.210208164830512	miRNATAP	-0.293670436231765	6.94877226450165e-32		NA	NA	NA
hsa-miR-21-5p	TTC33	-0.142759573450327	0.928848736959803	0.0664363069480789	0.854146273634859	miRNAWalker2_validate	-0.282581742421259	3.88747839795257e-42		NA	NA	NA
hsa-miR-21-5p	UBE4A	-0.142759573450327	0.928848736959803	0.0372435234099884	0.947672234633391	MirTarget;miRNATAP	-0.168760265780093	2.63068843528021e-19		NA	NA	NA
hsa-miR-21-5p	UBR3	-0.142759573450327	0.928848736959803	-0.189888740862493	0.716474081374667	miRNAWalker2_validate;MirTarget;miRNATAP	-0.294363758773514	5.80124674467305e-36		NA	NA	NA
hsa-miR-21-5p	UHMK1	-0.142759573450327	0.928848736959803	-0.532388644879841	0.193869381638697	mirMAP	-0.222415346334735	0.000210223419522674		NA	NA	NA
hsa-miR-21-5p	UNC80	-0.142759573450327	0.928848736959803	1.5180493916308	0.000245492162701072	MirTarget	-0.363774902177116	1.84904351115534e-06		NA	NA	NA
hsa-miR-21-5p	USP34	-0.142759573450327	0.928848736959803	0.31983481973417	0.619541330093891	miRNAWalker2_validate	-0.196058475100826	1.31129212224992e-22		NA	NA	NA
hsa-miR-21-5p	USP47	-0.142759573450327	0.928848736959803	-0.398675780084225	0.505168481323408	miRNAWalker2_validate	-0.169271433383096	2.9631118519651e-22		NA	NA	NA
hsa-miR-21-5p	UTRN	-0.142759573450327	0.928848736959803	-0.353162400847255	0.569693621125837	miRNAWalker2_validate	-0.470139987933755	3.6427348192286e-39		NA	NA	NA
hsa-miR-21-5p	VPS13A	-0.142759573450327	0.928848736959803	-0.137837777756072	0.760519147178311	miRNAWalker2_validate	-0.284157950575656	1.94094414412145e-20		NA	NA	NA
hsa-miR-21-5p	VPS13B	-0.142759573450327	0.928848736959803	-0.516930281966707	0.319742167805127	mirMAP	-0.119463943041318	5.19837809674789e-05		NA	NA	NA
hsa-miR-21-5p	VPS26A	-0.142759573450327	0.928848736959803	0.166468946107958	0.769107314640669	miRNAWalker2_validate	-0.102820257316725	5.79628755566504e-11		NA	NA	NA
hsa-miR-21-5p	VPS36	-0.142759573450327	0.928848736959803	-0.161551815252873	0.756336224060604	miRNAWalker2_validate	-0.254135515722118	1.05894378025598e-36		NA	NA	NA
hsa-miR-21-5p	VPS53	-0.142759573450327	0.928848736959803	-0.0306050114541607	0.950980757476722	mirMAP	-0.115212328214368	5.42397385050431e-14		NA	NA	NA
hsa-miR-21-5p	VPS54	-0.142759573450327	0.928848736959803	0.521506996105732	0.272691772084969	miRNAWalker2_validate	-0.17377178401262	3.93322016702974e-18		NA	NA	NA
hsa-miR-21-5p	WDR7	-0.142759573450327	0.928848736959803	-0.379300541586834	0.374640302028642	miRNAWalker2_validate	-0.180698862319217	1.27006834936194e-18		NA	NA	NA
hsa-miR-21-5p	WNK1	-0.142759573450327	0.928848736959803	0.0497976780393241	0.944394703690496	miRNAWalker2_validate	-0.126676800755744	2.62566085278265e-07		NA	NA	NA
hsa-miR-21-5p	WNK3	-0.142759573450327	0.928848736959803	1.6564805876707	4.0706077604075e-11	miRNAWalker2_validate	-0.387261682088303	1.64774796083302e-09		NA	NA	NA
hsa-miR-21-5p	WWC2	-0.142759573450327	0.928848736959803	-0.707654298913001	0.110488659133669	miRNAWalker2_validate	-0.198974767729545	1.3114686290144e-06		NA	NA	NA
hsa-miR-21-5p	XPO4	-0.142759573450327	0.928848736959803	0.133692722059218	0.769004025750918	mirMAP	-0.16262087766467	3.67257985410033e-13		NA	NA	NA
hsa-miR-21-5p	YAP1	-0.142759573450327	0.928848736959803	0.439916174496487	0.486618018811062	MirTarget;miRNATAP	-0.325756536921103	2.08121491758625e-26		NA	NA	NA
hsa-miR-21-5p	YPEL2	-0.142759573450327	0.928848736959803	-0.922339457139357	0.0540723831404196	mirMAP	-0.191453030914219	1.53802406846675e-10		NA	NA	NA
hsa-miR-21-5p	ZADH2	-0.142759573450327	0.928848736959803	-0.421791054059947	0.400573736120299	miRNAWalker2_validate;mirMAP	-0.236140452123295	1.61465154504701e-27		NA	NA	NA
hsa-miR-21-5p	ZBTB20	-0.142759573450327	0.928848736959803	-0.0187281891850804	0.920873287876871	miRNAWalker2_validate	-0.512160134920868	2.50160661161994e-29		NA	NA	NA
hsa-miR-21-5p	ZBTB38	-0.142759573450327	0.928848736959803	-0.0543247981068697	0.923623989342764	miRNAWalker2_validate	-0.292897687469219	4.97513187822961e-29		NA	NA	NA
hsa-miR-21-5p	ZBTB47	-0.142759573450327	0.928848736959803	-0.34886522302334	0.433081541452101	miRNAWalker2_validate	-0.168184830331374	9.33581199668469e-13		NA	NA	NA
hsa-miR-21-5p	ZDHHC17	-0.142759573450327	0.928848736959803	-0.465271049676388	0.308620746358459	miRNATAP	-0.288627519195431	2.26497977409304e-41		NA	NA	NA
hsa-miR-21-5p	ZFP36L2	-0.142759573450327	0.928848736959803	0.351373563498499	0.634451620860658	MirTarget	-0.198642857440537	8.52276974415143e-10		NA	NA	NA
hsa-miR-21-5p	ZMYM2	-0.142759573450327	0.928848736959803	-0.239436835815108	0.675880240350587	miRNAWalker2_validate	-0.127847914107643	4.1156900196522e-08		NA	NA	NA
hsa-miR-21-5p	ZNF208	-0.142759573450327	0.928848736959803	-0.531444878355607	0.0569110421767705	mirMAP	-0.292098132572268	2.73952563823386e-05		NA	NA	NA
hsa-miR-21-5p	ZNF24	-0.142759573450327	0.928848736959803	-0.610911888595767	0.338749143970225	mirMAP	-0.125588812465726	1.77902479130186e-07		NA	NA	NA
hsa-miR-21-5p	ZNF280D	-0.142759573450327	0.928848736959803	-0.435094266137722	0.35359418283962	mirMAP	-0.274915560298223	6.6200396195592e-43		NA	NA	NA
hsa-miR-21-5p	ZNF292	-0.142759573450327	0.928848736959803	0.29890968035793	0.547857819043514	miRNAWalker2_validate	-0.228561666670143	1.72761239251222e-15		NA	NA	NA
hsa-miR-21-5p	ZNF449	-0.142759573450327	0.928848736959803	-0.149232394069184	0.600725506984284	MirTarget	-0.155315166183741	2.94822810873481e-15		NA	NA	NA
hsa-miR-21-5p	ZNF451	-0.142759573450327	0.928848736959803	0.0687114560654551	0.881435731516892	mirMAP	-0.198706768089808	3.54655918991468e-30		NA	NA	NA
hsa-miR-21-5p	ZNF527	-0.142759573450327	0.928848736959803	0.0447756821659486	0.857188667373526	MirTarget	-0.182678619109294	3.86904549803528e-24		NA	NA	NA
hsa-miR-21-5p	ZNF654	-0.142759573450327	0.928848736959803	-0.116434967022939	0.758038483378276	miRNATAP	-0.173363422506433	8.99558510587105e-17		NA	NA	NA
hsa-miR-21-5p	ZNF667	-0.142759573450327	0.928848736959803	1.19539433462433	7.48681212305886e-06	miRNAWalker2_validate	-0.428272360487598	5.30837794649517e-14		NA	NA	NA
hsa-miR-21-5p	ZNF680	-0.142759573450327	0.928848736959803	-0.733853448892181	0.0560670784896392	MirTarget	-0.13816350545046	1.61216030110629e-06		NA	NA	NA
hsa-miR-21-5p	ZNF704	-0.142759573450327	0.928848736959803	-0.676747931662059	0.0568076478498075	MirTarget;miRNATAP	-0.383066314653317	5.420261673963e-15		NA	NA	NA
hsa-miR-21-5p	ZRANB1	-0.142759573450327	0.928848736959803	0.0509131719926321	0.923187013758188	miRNAWalker2_validate	-0.202730374513769	1.19486880747037e-35		NA	NA	NA
hsa-miR-21-5p	ZSWIM6	-0.142759573450327	0.928848736959803	-0.459829863607944	0.232476837353957	MirTarget;miRNATAP	-0.134737304178267	4.86561659146438e-07		NA	NA	NA
hsa-miR-21-5p	ZYG11B	-0.142759573450327	0.928848736959803	0.0843632235255747	0.863405622848021	miRNAWalker2_validate;mirMAP	-0.237515753823477	2.72630953319275e-31		NA	NA	NA