miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-140-3p ABL1 -0.123864258867705 0.14796686619449 0.0143957445246503 0.802818620463351 MirTarget;PITA -0.108292549966284 4.03747363160958e-05 NA NA NA hsa-miR-149-5p ABL1 -0.0535821592029952 0.657713892012548 0.0143957445246503 0.802818620463351 miRNAWalker2_validate -0.149998583909469 8.50330650278858e-28 NA NA NA hsa-miR-29a-3p ABL1 -0.224140851576083 0.0349516103550236 0.0143957445246503 0.802818620463351 miRNAWalker2_validate;miRNATAP -0.129389826504859 4.38987333540906e-10 NA NA NA hsa-miR-29a-5p ABL1 -0.0697727833812678 0.350621682973119 0.0143957445246503 0.802818620463351 MirTarget -0.110556620114591 1.96554256170541e-06 NA NA NA hsa-miR-29b-3p ABL1 -0.178522712044497 0.0856106432165465 0.0143957445246503 0.802818620463351 miRNATAP -0.183750024397894 1.77278201614351e-25 NA NA NA hsa-miR-29c-3p ABL1 -0.372180390387259 0.00202634431973208 0.0143957445246503 0.802818620463351 miRNATAP -0.147792143048995 5.28105050732972e-23 NA NA NA hsa-miR-30a-5p ABL1 -0.062942936611238 0.513851954700252 0.0143957445246503 0.802818620463351 miRNAWalker2_validate;MirTarget;miRNATAP -0.235909063634104 1.3973277936456e-16 23287430 In K562 leukemia cells overexpression of miR-30a reduced ABL1 and BCR-ABL1 protein expression decreased proliferation and arrested cell cycle progression between G1 and S; These findings strongly suggest that miR-30a acts as a tumor suppressor by downregulating ABL1 and BCR-ABL1 expression progression chronic myeloid leukemia hsa-miR-30b-5p ABL1 -0.0858101016322106 0.263243418521854 0.0143957445246503 0.802818620463351 MirTarget;miRNATAP -0.120907821952767 6.32750624886575e-06 NA NA NA hsa-miR-30c-5p ABL1 -0.0469114469943097 0.565934557464627 0.0143957445246503 0.802818620463351 MirTarget;miRNATAP -0.149123058508228 2.48075647790404e-08 NA NA NA hsa-miR-30d-5p ABL1 -0.0607010868720046 0.471410043548794 0.0143957445246503 0.802818620463351 MirTarget;miRNATAP -0.139463869338977 5.35496466278748e-06 NA NA NA hsa-miR-3127-5p ABL1 -0.0645479321229978 0.382330591204685 0.0143957445246503 0.802818620463351 MirTarget -0.107898733709439 3.77555360940339e-06 NA NA NA hsa-miR-342-3p ABL1 -0.125683829770877 0.105810751640137 0.0143957445246503 0.802818620463351 miRNAWalker2_validate -0.128206724043204 1.6799883909592e-07 NA NA NA hsa-miR-361-5p ABL1 0.0107519600643302 0.882230614095565 0.0143957445246503 0.802818620463351 miRanda -0.174366067528748 1.67168382685165e-09 NA NA NA hsa-miR-484 ABL1 -0.0190828819137732 0.802298900989652 0.0143957445246503 0.802818620463351 miRNAWalker2_validate -0.162527559241345 6.87631889536367e-12 NA NA NA hsa-miR-488-3p ABL1 -0.257955761012568 0.00280795162806256 0.0143957445246503 0.802818620463351 mirMAP -0.107458785475156 8.58266909621695e-08 NA NA NA hsa-miR-26b-5p ANAPC1 -0.0984536538762768 0.231803769574623 0.0691250041984306 0.169395272045304 miRNAWalker2_validate -0.114244018054199 3.87608907795491e-05 NA NA NA hsa-miR-30a-5p ANAPC5 -0.062942936611238 0.513851954700252 -0.0360786424414208 0.432061025793839 miRNAWalker2_validate -0.12923684901967 5.06612053518959e-10 NA NA NA hsa-miR-125a-5p ATM -0.0833349424517387 0.325440200253607 -0.0447507082085021 0.439654845969846 miRanda -0.191891258396068 1.08085791159268e-10 NA NA NA hsa-miR-146b-5p ATM 0.441078059667297 0.000237619929889385 -0.0447507082085021 0.439654845969846 miRanda -0.139090363114708 3.02172223504569e-15 27602131 The role of microRNA 146b miR-146b in ATC remains to be elucidated; In order to characterize the role of miR-146b in ATC overexpression or interference of miR-146b was induced in ATC cell lines and cell proliferation and migration were evaluated; The potential targets of miR-146b were searched in the Gene Expression Omnibus database for ATC and matched non-tumor control samples; The expression level of potential targets was detected following overexpression or interference of miR-146b in ATC cell lines; In addition cell migration of ATC was also affected by miR-146b; During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis; In conclusion p21 may participate in the regulation of ATC cell proliferation by miR-146b cell migration;progression thyroid cancer hsa-miR-186-5p ATM 0.0606381894130505 0.514217717592464 -0.0447507082085021 0.439654845969846 mirMAP -0.123208669272272 4.47711357729956e-07 NA NA NA hsa-miR-18a-5p ATM 0.175320895022849 0.0732549187136286 -0.0447507082085021 0.439654845969846 miRNAWalker2_validate;miRTarBase;MirTarget -0.104121682157558 6.75084520132396e-07 23437304;25963391;23857602;23229340 MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression colorectal cancer;cervical and endocervical cancer;breast cancer;colon cancer hsa-miR-26b-5p ATM -0.0984536538762768 0.231803769574623 -0.0447507082085021 0.439654845969846 MirTarget -0.123652362350772 3.89261799231929e-05 NA NA NA hsa-miR-30b-5p ATM -0.0858101016322106 0.263243418521854 -0.0447507082085021 0.439654845969846 mirMAP -0.145142243457673 7.69838249932177e-06 25202329 However specific miRNAs are downregulated in ATC such as those of the miR-200 and miR-30 families which are important negative regulators of cell migration invasion and epithelial-to-mesenchymal transition EMT processes that are overactivated in ATC cell migration thyroid cancer hsa-miR-30c-5p ATM -0.0469114469943097 0.565934557464627 -0.0447507082085021 0.439654845969846 mirMAP -0.21095555767297 5.90764153731747e-11 NA NA NA hsa-miR-30d-3p ATM 0.0854049347792898 0.20691254801121 -0.0447507082085021 0.439654845969846 mirMAP -0.108465899695928 0.000445378990366815 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-30d-5p ATM -0.0607010868720046 0.471410043548794 -0.0447507082085021 0.439654845969846 mirMAP -0.1256777304023 0.000751902317966877 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-324-5p ATM 0.0183370482495953 0.842996782677271 -0.0447507082085021 0.439654845969846 miRanda -0.136864230463836 1.45860904388815e-09 NA NA NA hsa-miR-339-5p ATM 0.273253751377152 0.0185300170790638 -0.0447507082085021 0.439654845969846 miRanda -0.10715400072152 1.20256225486914e-09 NA NA NA hsa-miR-500a-5p ATM 0.0634452729978863 0.396072382601677 -0.0447507082085021 0.439654845969846 mirMAP -0.13339851803771 1.47786050929078e-06 NA NA NA hsa-miR-766-3p ATM -0.147747825439875 0.266658981351507 -0.0447507082085021 0.439654845969846 MirTarget -0.100027991553697 4.5702698390424e-11 NA NA NA hsa-miR-139-5p BUB1 -0.647754756897633 2.15378307237163e-06 1.4351194337037 1.02179760833826e-20 miRanda -0.36081871787807 3.0472333231544e-12 NA NA NA hsa-miR-495-3p BUB1 -0.421982588962504 0.00504424038526901 1.4351194337037 1.02179760833826e-20 MirTarget -0.181066473688787 9.68455691094411e-05 NA NA NA hsa-miR-543 BUB1 -0.347737549779625 0.0157394585079683 1.4351194337037 1.02179760833826e-20 miRanda -0.165142840208375 0.000868833355472374 NA NA NA hsa-miR-192-5p BUB1B -0.0426540615456616 0.621616927627554 1.50728439672711 1.98268442027891e-18 miRNAWalker2_validate -0.273477403551104 0.00950616774231799 NA NA NA hsa-miR-22-3p BUB1B 0.0277287593466351 0.809325106832648 1.50728439672711 1.98268442027891e-18 miRNAWalker2_validate -0.296855879536619 0.00161354583170632 NA NA NA hsa-let-7d-5p BUB3 0.111345229817909 0.0872634398336815 -0.0117251104567817 0.814707249620494 miRNAWalker2_validate -0.124433445452519 4.4299977170404e-05 NA NA NA hsa-miR-107 BUB3 -0.121070851008387 0.198381281894242 -0.0117251104567817 0.814707249620494 miRanda -0.1176157283119 3.590711301107e-11 NA NA NA hsa-miR-146b-5p BUB3 0.441078059667297 0.000237619929889385 -0.0117251104567817 0.814707249620494 miRanda -0.125173108534517 1.65889939800852e-20 NA NA NA hsa-miR-339-5p BUB3 0.273253751377152 0.0185300170790638 -0.0117251104567817 0.814707249620494 miRanda -0.100744338607026 9.00394356329357e-14 NA NA NA hsa-miR-421 BUB3 -0.171573235029879 0.0518112933651029 -0.0117251104567817 0.814707249620494 miRanda -0.143462672277728 6.99454239209502e-16 NA NA NA hsa-miR-590-3p BUB3 0.312951515438396 0.000480455423843996 -0.0117251104567817 0.814707249620494 miRanda;mirMAP -0.118563607511374 1.47914371418344e-11 NA NA NA hsa-miR-7-1-3p BUB3 -0.0894475316162393 0.37322359785108 -0.0117251104567817 0.814707249620494 mirMAP -0.102152824412418 1.1288406512277e-10 NA NA NA hsa-miR-769-3p BUB3 -0.287263649023213 0.018145224330845 -0.0117251104567817 0.814707249620494 PITA;miRNATAP -0.120165480892171 2.62797821571282e-21 NA NA NA hsa-miR-320a CCNA1 0.092135599636725 0.243028758989929 -0.0345751119356932 0.856250718274326 miRanda -0.419262966622663 0.00155608491843828 NA NA NA hsa-miR-129-5p CCNA2 -0.567133990117126 0.00116152215679936 1.13046163382069 6.2750224400998e-22 miRanda -0.1950549250526 2.32918092265595e-10 NA NA NA hsa-miR-132-3p CCNA2 -0.178379362833856 0.13335200877129 1.13046163382069 6.2750224400998e-22 miRNAWalker2_validate -0.198614572840467 2.8552594520693e-05 NA NA NA hsa-miR-218-5p CCNA2 -0.534287404275829 0.00308322441944796 1.13046163382069 6.2750224400998e-22 MirTarget -0.139146757684148 1.83093488368657e-06 NA NA NA hsa-miR-22-3p CCNA2 0.0277287593466351 0.809325106832648 1.13046163382069 6.2750224400998e-22 MirTarget -0.233826318997895 0.000281514452087732 25596928 The sequence of miR-22 which is conserved in mice rats humans and other mammalians aligns with the sequence of 3'-UTR of CCNA2; Chenodeoxycholic acid treatment and miR-22 mimics reduced CCNA2 protein and increased the number of G0/G1 Huh7 and HCT116 cells; In humans the expression levels of miR-22 and CCNA2 are inversely correlated in liver and colon cancers liver cancer hsa-miR-29a-3p CCNA2 -0.224140851576083 0.0349516103550236 1.13046163382069 6.2750224400998e-22 MirTarget -0.48507954906838 4.41915243409641e-14 NA NA NA hsa-miR-29b-3p CCNA2 -0.178522712044497 0.0856106432165465 1.13046163382069 6.2750224400998e-22 MirTarget -0.398844731810031 1.81500387990034e-12 NA NA NA hsa-miR-29c-3p CCNA2 -0.372180390387259 0.00202634431973208 1.13046163382069 6.2750224400998e-22 MirTarget -0.397061734405458 5.18003750451598e-17 NA NA NA hsa-miR-374b-5p CCNA2 -0.210113949579608 0.0056866083818614 1.13046163382069 6.2750224400998e-22 mirMAP -0.262535649334863 0.00100261285198039 NA NA NA hsa-miR-508-3p CCNA2 -0.292045725850845 0.0158994578296519 1.13046163382069 6.2750224400998e-22 MirTarget;PITA;miRNATAP -0.145554780156457 0.000918119803217115 NA NA NA hsa-miR-139-5p CCNB1 -0.647754756897633 2.15378307237163e-06 0.84976221863878 1.34547967203245e-21 miRanda -0.158522988130583 1.297334982042e-07 NA NA NA hsa-miR-339-5p CCNB3 0.273253751377152 0.0185300170790638 -0.234870852552629 0.00599558926982139 miRanda -0.176872777316358 3.1676352111193e-08 NA NA NA hsa-miR-361-5p CCNB3 0.0107519600643302 0.882230614095565 -0.234870852552629 0.00599558926982139 miRanda -0.221101186108731 0.000565419134065904 NA NA NA hsa-miR-590-3p CCNB3 0.312951515438396 0.000480455423843996 -0.234870852552629 0.00599558926982139 miRanda -0.182806767559893 1.10227582601784e-05 NA NA NA hsa-let-7e-5p CCND1 0.0471189209570344 0.657255455444655 0.517304800763483 1.04046594178888e-06 miRTarBase;miRNATAP -0.228941875441154 1.78800254985128e-06 NA NA NA hsa-let-7i-5p CCND1 0.0952853587923244 0.192120688957249 0.517304800763483 1.04046594178888e-06 miRNATAP -0.236343082962889 0.00183350614645 NA NA NA hsa-miR-1266-5p CCND1 0.313515127917259 0.0138664194727392 0.517304800763483 1.04046594178888e-06 MirTarget -0.112027246009442 0.00108554865163972 NA NA NA hsa-miR-135a-5p CCND1 -0.122734612943241 0.196985964641886 0.517304800763483 1.04046594178888e-06 mirMAP -0.247492714890572 1.3238406350361e-07 NA NA NA hsa-miR-23b-3p CCND1 0.0476610245860094 0.630277683372344 0.517304800763483 1.04046594178888e-06 miRNATAP -0.279243855267394 9.57342364736838e-09 NA NA NA hsa-miR-26b-5p CCND1 -0.0984536538762768 0.231803769574623 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate -0.171067179820866 0.00692173397836684 NA NA NA hsa-miR-29a-3p CCND1 -0.224140851576083 0.0349516103550236 0.517304800763483 1.04046594178888e-06 mirMAP -0.23358540897967 1.12603356998685e-05 NA NA NA hsa-miR-29b-3p CCND1 -0.178522712044497 0.0856106432165465 0.517304800763483 1.04046594178888e-06 mirMAP -0.30608004962895 3.25721913642118e-11 NA NA NA hsa-miR-29c-3p CCND1 -0.372180390387259 0.00202634431973208 0.517304800763483 1.04046594178888e-06 mirMAP -0.263497298429692 1.34805822955252e-11 NA NA NA hsa-miR-3065-5p CCND1 -0.262608140481507 0.0414570974148034 0.517304800763483 1.04046594178888e-06 mirMAP -0.105747126080182 0.00178155205723976 NA NA NA hsa-miR-330-3p CCND1 -0.427388106938151 0.00300489284414266 0.517304800763483 1.04046594178888e-06 mirMAP -0.156757729272097 1.33987981854083e-07 NA NA NA hsa-miR-338-3p CCND1 -0.39963704679384 0.0034410643220691 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase;miRanda -0.138060683824931 7.24806702911648e-05 NA NA NA hsa-miR-34a-5p CCND1 0.143472919835524 0.211283228567057 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.176667405034615 4.78965946188406e-06 25792709;21399894 This inhibition of proliferation was associated with a decrease in cyclin D1 levels orchestrated principally by HNF-4α a target of miR-34a considered to act as a tumour suppressor in the liver;Quantitative PCR and western analysis confirmed decreased expression of two genes BCL-2 and CCND1 in docetaxel-resistant cells which are both targeted by miR-34a ; liver cancer;breast cancer hsa-miR-365a-3p CCND1 -0.184222766207919 0.0292663235724171 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate;miRTarBase -0.195991913128339 0.000198680829195249 NA NA NA hsa-miR-425-5p CCND1 -0.0412837711174561 0.682284485592858 0.517304800763483 1.04046594178888e-06 miRNAWalker2_validate -0.134644465621285 0.00325561137510874 NA NA NA hsa-miR-490-3p CCND1 -0.834268051506884 5.26203010743215e-06 0.517304800763483 1.04046594178888e-06 miRanda -0.131117175699242 2.90415525218072e-08 24440705 MicroRNA 490 3p inhibits proliferation of A549 lung cancer cells by targeting CCND1; We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1 which plays a key role in G1/S phase transition; In addition the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3'UTR lung cancer hsa-miR-495-3p CCND1 -0.421982588962504 0.00504424038526901 0.517304800763483 1.04046594178888e-06 MirTarget -0.136090131725584 2.1911780731285e-06 NA NA NA hsa-miR-7-1-3p CCND1 -0.0894475316162393 0.37322359785108 0.517304800763483 1.04046594178888e-06 mirMAP -0.241924898170475 2.08913227927897e-08 NA NA NA hsa-miR-769-3p CCND1 -0.287263649023213 0.018145224330845 0.517304800763483 1.04046594178888e-06 mirMAP -0.276564457533319 1.97356475814615e-15 NA NA NA hsa-miR-885-5p CCND1 -0.423614695846868 1.14213289528402e-05 0.517304800763483 1.04046594178888e-06 miRNATAP -0.292419966156513 1.08197956283879e-10 NA NA NA hsa-let-7b-3p CCND2 -0.00495618733298731 0.952288980686967 0.278072058804804 0.00230941164668614 mirMAP -0.124812140552693 0.00599796857804289 NA NA NA hsa-let-7f-1-3p CCND2 0.110713086694053 0.21484524300518 0.278072058804804 0.00230941164668614 mirMAP -0.122537465829283 0.00233902672067921 NA NA NA hsa-let-7g-5p CCND2 -0.0253396334608382 0.742556489443668 0.278072058804804 0.00230941164668614 miRNATAP -0.196434601966773 0.0012996932102575 NA NA NA hsa-let-7i-5p CCND2 0.0952853587923244 0.192120688957249 0.278072058804804 0.00230941164668614 miRNATAP -0.162156277541767 0.00988534673137717 NA NA NA hsa-miR-129-5p CCND2 -0.567133990117126 0.00116152215679936 0.278072058804804 0.00230941164668614 mirMAP -0.105272286982926 4.44505393807218e-07 NA NA NA hsa-miR-145-3p CCND2 0.110543172922298 0.294150620210019 0.278072058804804 0.00230941164668614 mirMAP -0.149944712694934 9.82587587919985e-06 NA NA NA hsa-miR-145-5p CCND2 0.108948399943015 0.310032352776693 0.278072058804804 0.00230941164668614 miRNATAP -0.155654659327631 1.04593167784463e-05 NA NA NA hsa-miR-146b-3p CCND2 0.283486177568242 0.0118918188864879 0.278072058804804 0.00230941164668614 MirTarget;PITA;miRNATAP -0.117766891479947 0.000283548163350027 NA NA NA hsa-miR-150-5p CCND2 0.0645271721519611 0.513295658828718 0.278072058804804 0.00230941164668614 mirMAP -0.149451068560082 7.57763104304709e-05 NA NA NA hsa-miR-151a-3p CCND2 0.0336074122085215 0.726834677736803 0.278072058804804 0.00230941164668614 mirMAP -0.12420907434582 0.00497911559673839 NA NA NA hsa-miR-185-5p CCND2 -0.0767432095218297 0.404113540276816 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.183398276514872 8.3630357382723e-06 NA NA NA hsa-miR-186-5p CCND2 0.0606381894130505 0.514217717592464 0.278072058804804 0.00230941164668614 mirMAP;miRNATAP -0.133720175872038 0.00173369326890833 NA NA NA hsa-miR-191-5p CCND2 0.0973986771912738 0.220078671774056 0.278072058804804 0.00230941164668614 MirTarget -0.20451499794437 0.00023723661594718 NA NA NA hsa-miR-26a-5p CCND2 0.033823776747294 0.726610414342369 0.278072058804804 0.00230941164668614 miRNAWalker2_validate;miRTarBase;mirMAP;miRNATAP -0.122521188171157 0.00669669430395561 NA NA NA hsa-miR-26b-5p CCND2 -0.0984536538762768 0.231803769574623 0.278072058804804 0.00230941164668614 mirMAP;miRNATAP -0.21765809461179 3.01296451472833e-05 NA NA NA hsa-miR-28-5p CCND2 0.104003572105781 0.153594705579534 0.278072058804804 0.00230941164668614 miRanda -0.151040812510917 0.00458703218164268 NA NA NA hsa-miR-29a-3p CCND2 -0.224140851576083 0.0349516103550236 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.199711571525784 5.61968185759315e-06 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29a-5p CCND2 -0.0697727833812678 0.350621682973119 0.278072058804804 0.00230941164668614 mirMAP -0.166759307844904 0.000688470083956136 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29b-3p CCND2 -0.178522712044497 0.0856106432165465 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.202303651104096 1.45292987304036e-07 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-29c-3p CCND2 -0.372180390387259 0.00202634431973208 0.278072058804804 0.00230941164668614 MirTarget;miRNATAP -0.239386359038839 8.64180673949139e-14 NA NA NA hsa-miR-30d-3p CCND2 0.0854049347792898 0.20691254801121 0.278072058804804 0.00230941164668614 mirMAP -0.155355754639963 0.00382748112364014 NA NA NA hsa-miR-30e-3p CCND2 0.00173769765874887 0.984965501547409 0.278072058804804 0.00230941164668614 mirMAP -0.268522462794922 4.02496537819957e-07 NA NA NA hsa-miR-324-3p CCND2 -0.0103367134443655 0.904494071318044 0.278072058804804 0.00230941164668614 miRNAWalker2_validate -0.184828101121747 1.93642919504512e-05 NA NA NA hsa-miR-331-5p CCND2 0.00538076980695534 0.942833685963252 0.278072058804804 0.00230941164668614 miRNATAP -0.168360380366874 0.000470859110297375 NA NA NA hsa-miR-33a-3p CCND2 -0.158366376619191 0.0991512910387226 0.278072058804804 0.00230941164668614 MirTarget -0.10197096016646 0.00681801603426165 NA NA NA hsa-miR-3622a-3p CCND2 0.0389203848593471 0.75605812962459 0.278072058804804 0.00230941164668614 mirMAP -0.125163354365051 1.78891207109425e-05 NA NA NA hsa-miR-488-3p CCND2 -0.257955761012568 0.00280795162806256 0.278072058804804 0.00230941164668614 mirMAP -0.183392273105331 1.52514393662224e-05 NA NA NA hsa-miR-488-5p CCND2 -0.385755602369049 7.99431673275965e-08 0.278072058804804 0.00230941164668614 mirMAP -0.153852506035559 0.00315722783501446 NA NA NA hsa-miR-500a-5p CCND2 0.0634452729978863 0.396072382601677 0.278072058804804 0.00230941164668614 mirMAP -0.155381019814906 0.0013241335866013 NA NA NA hsa-miR-501-5p CCND2 0.0878951859817452 0.309663377222443 0.278072058804804 0.00230941164668614 PITA;mirMAP;miRNATAP -0.110941849752897 0.00801840144207187 NA NA NA hsa-miR-660-5p CCND2 -0.138989893523975 0.0945491063873448 0.278072058804804 0.00230941164668614 mirMAP -0.238701834193771 2.94573193719288e-07 NA NA NA hsa-miR-7-1-3p CCND2 -0.0894475316162393 0.37322359785108 0.278072058804804 0.00230941164668614 mirMAP -0.150925481137646 2.69591030726187e-05 NA NA NA hsa-miR-192-5p CCNE1 -0.0426540615456616 0.621616927627554 0.253536339251842 6.27968052141139e-05 miRNAWalker2_validate -0.134085089321587 0.000290914914800468 NA NA NA hsa-miR-195-5p CCNE1 -0.00728332132631593 0.92969850577609 0.253536339251842 6.27968052141139e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.187859421689644 1.50466938767298e-07 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-497-5p CCNE1 -0.000783764887886562 0.991703566618871 0.253536339251842 6.27968052141139e-05 MirTarget;miRNATAP -0.107990353660474 0.00593270549342151 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-30a-5p CCNE2 -0.062942936611238 0.513851954700252 0.318474669342545 0.000263822187487161 miRNATAP -0.404174405891182 3.32123969313609e-10 NA NA NA hsa-miR-30d-5p CCNE2 -0.0607010868720046 0.471410043548794 0.318474669342545 0.000263822187487161 miRNATAP -0.248704351406826 0.000276173784789898 25843294 MicroRNA 30d 5p inhibits tumour cell proliferation and motility by directly targeting CCNE2 in non small cell lung cancer; In addition the re-introduction of CCNE2 expression antagonised the inhibitory effects of miR-30d-5p on the capacity of NSCLC cells for proliferation and motility motility lung squamous cell cancer hsa-let-7a-3p CDC14B 0.0530563276543763 0.370749834463566 -0.00418796279434552 0.932601406782922 miRNATAP -0.109328106203707 0.000398026457615324 NA NA NA hsa-miR-103a-2-5p CDC14B -0.0677071239234235 0.496202425310213 -0.00418796279434552 0.932601406782922 MirTarget -0.102604047401722 2.67298628193792e-10 NA NA NA hsa-miR-140-5p CDC14B 0.065063507103468 0.380452509211773 -0.00418796279434552 0.932601406782922 miRanda -0.115919534732884 1.09926891917265e-06 NA NA NA hsa-miR-15a-5p CDC14B 0.142658985373611 0.0536219185009004 -0.00418796279434552 0.932601406782922 miRNAWalker2_validate;MirTarget -0.106792439599766 6.29352363671128e-05 NA NA NA hsa-miR-26b-5p CDC14B -0.0984536538762768 0.231803769574623 -0.00418796279434552 0.932601406782922 miRNAWalker2_validate -0.130393401071805 3.1221123624615e-08 NA NA NA hsa-miR-30e-3p CDC14B 0.00173769765874887 0.984965501547409 -0.00418796279434552 0.932601406782922 MirTarget -0.139875306109506 5.49999742311063e-09 NA NA NA hsa-miR-576-5p CDC14B 0.106482276040047 0.194379152564166 -0.00418796279434552 0.932601406782922 mirMAP -0.102920250415792 2.85043620837935e-07 NA NA NA hsa-miR-590-5p CDC14B 0.16946027035949 0.0386004382081678 -0.00418796279434552 0.932601406782922 miRanda -0.100191027610528 5.79389513815854e-07 NA NA NA hsa-miR-628-5p CDC14B -0.0469793532265896 0.657420003383685 -0.00418796279434552 0.932601406782922 miRNATAP -0.101796733698449 5.39598160064348e-11 NA NA NA hsa-miR-192-5p CDC20 -0.0426540615456616 0.621616927627554 1.46034936169535 2.72638407111505e-21 miRNAWalker2_validate -0.272964968782111 0.00406311762213126 NA NA NA hsa-miR-30a-5p CDC20 -0.062942936611238 0.513851954700252 1.46034936169535 2.72638407111505e-21 miRNAWalker2_validate -0.456434933466446 0.000150737030766859 NA NA NA hsa-miR-98-5p CDC23 -0.0806971683871618 0.251777941211683 0.0577357778017227 0.139300051188219 miRNAWalker2_validate -0.114624567425978 7.72764419682718e-10 NA NA NA hsa-let-7c-5p CDC25A -0.04295851886768 0.676052842748691 0.646620424698645 1.11582796563891e-12 MirTarget -0.214960663503888 6.76241462573345e-05 25909324 MicroRNA let 7c Inhibits Cell Proliferation and Induces Cell Cycle Arrest by Targeting CDC25A in Human Hepatocellular Carcinoma; The aim of the present study was to determine whether the cell cycle regulator CDC25A is involved in the antitumor effect of let-7c in HCC; The luciferase reporter assay showed that CDC25A was a direct target of let-7c and that let-7c inhibited the expression of CDC25A protein by directly targeting its 3' UTR; In conclusion this study indicates that let-7c suppresses HCC progression possibly by directly targeting the cell cycle regulator CDC25A and indirectly affecting its downstream target molecules progression liver cancer hsa-let-7e-5p CDC25A 0.0471189209570344 0.657255455444655 0.646620424698645 1.11582796563891e-12 MirTarget;miRNATAP -0.167008236424579 0.000179786256441597 NA NA NA hsa-miR-103a-3p CDC25A -0.147443193527362 0.128400914654149 0.646620424698645 1.11582796563891e-12 miRNAWalker2_validate -0.344742934929935 5.35464435612012e-07 NA NA NA hsa-miR-107 CDC25A -0.121070851008387 0.198381281894242 0.646620424698645 1.11582796563891e-12 miRanda -0.125696533286471 0.00562700552117093 NA NA NA hsa-miR-184 CDC25A -0.842534984406592 6.08844932016991e-08 0.646620424698645 1.11582796563891e-12 miRanda -0.171449855859938 1.09030326802242e-11 25990966;27083050 Wild-type or mutant CDC25A promoters were constructed by PCR and site-directed mutagenesis to verify whether miR-184 could inhibit CDC25A expression at post-transcription level; Boyden chamber assay was used to assess whether miR-184 could modulate cell invasiveness via targeting CDC25A and c-Myc; MiR-184 suppressed CDC25A expression by enhancing the instability of its mRNA as a result of miR-184 binding to its coding region; An increase in CDC25A expression by means of a reduction in miR-184 promotes cell invasiveness; MiR-184 as a tumor suppressor miR inhibits cell proliferation and invasion capability via targeting CDC25A and c-Myc;We recently reported that miR-184 promotes tumor progression in non-small cell lung cancer via targeting CDC25A and c-Myc ;progression lung squamous cell cancer;lung cancer hsa-miR-192-5p CDC25A -0.0426540615456616 0.621616927627554 0.646620424698645 1.11582796563891e-12 miRNAWalker2_validate -0.146866680761457 0.0069257403285026 NA NA NA hsa-miR-346 CDC25A -0.577786425963268 2.10883270131593e-09 0.646620424698645 1.11582796563891e-12 miRanda -0.255432672074493 3.88596211908328e-10 NA NA NA hsa-miR-34a-5p CDC25A 0.143472919835524 0.211283228567057 0.646620424698645 1.11582796563891e-12 miRNAWalker2_validate;miRNATAP -0.135662631912312 0.0001568130894244 NA NA NA hsa-miR-484 CDC25A -0.0190828819137732 0.802298900989652 0.646620424698645 1.11582796563891e-12 miRNAWalker2_validate -0.166003052207091 0.00346608243662189 NA NA NA hsa-miR-98-5p CDC25A -0.0806971683871618 0.251777941211683 0.646620424698645 1.11582796563891e-12 miRNAWalker2_validate;MirTarget -0.194571776110002 0.00274650296545981 NA NA NA hsa-let-7a-5p CDC25B 0.00220017218203594 0.982661524826606 0.311976500963614 2.28977810170594e-07 miRNAWalker2_validate -0.10075917890523 0.00480731811035263 NA NA NA hsa-miR-27b-3p CDC25B -0.0599187745243146 0.58208143475992 0.311976500963614 2.28977810170594e-07 miRNATAP -0.112042646691932 4.20125028256148e-07 NA NA NA hsa-miR-744-5p CDC25B -0.31868579180387 0.000969037378618306 0.311976500963614 2.28977810170594e-07 miRNAWalker2_validate -0.198338532476978 1.47343736306093e-17 NA NA NA hsa-miR-101-3p CDC7 -0.132333931567244 0.241891922291381 0.404734256222451 4.57207893473647e-09 miRNAWalker2_validate -0.129963656309264 0.000121032102658647 NA NA NA hsa-miR-192-5p CDC7 -0.0426540615456616 0.621616927627554 0.404734256222451 4.57207893473647e-09 miRNAWalker2_validate -0.117464495369371 0.0031726768811549 NA NA NA hsa-miR-103a-3p CDK2 -0.147443193527362 0.128400914654149 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate -0.244529342446133 9.50719675121511e-05 NA NA NA hsa-miR-124-3p CDK2 -0.506026514549291 0.0078056370358648 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate;miRTarBase -0.168041487619791 1.69965544728722e-19 NA NA NA hsa-miR-490-3p CDK2 -0.834268051506884 5.26203010743215e-06 0.813366814153226 5.60398748524507e-23 miRanda -0.197884061603562 5.32644876787653e-25 NA NA NA hsa-miR-495-3p CDK2 -0.421982588962504 0.00504424038526901 0.813366814153226 5.60398748524507e-23 mirMAP -0.162294675651709 1.05494965345435e-11 NA NA NA hsa-miR-7-1-3p CDK2 -0.0894475316162393 0.37322359785108 0.813366814153226 5.60398748524507e-23 mirMAP -0.215889061968793 2.42828507111814e-09 NA NA NA hsa-miR-885-5p CDK2 -0.423614695846868 1.14213289528402e-05 0.813366814153226 5.60398748524507e-23 miRNAWalker2_validate;miRTarBase -0.319170723509576 1.73805882382479e-17 NA NA NA hsa-miR-124-3p CDK4 -0.506026514549291 0.0078056370358648 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.149095153225998 7.48747415901698e-17 25348738;25731732;27323123 MiR 124 retards bladder cancer growth by directly targeting CDK4; In order to investigate the physiological role of miR-124 in bladder cancer target genes of miR-124 were predicted by the TargetScan software and cyclin-dependent kinase CDK4 which has been implicated as a regulator of cell cycle was chosen for further study; MiR-124 could significantly repress CDK4 expression by targeting its binding site in the 3'UTR of CDK4 in vitro; In both bladder cancer cell lines and tissues the expression of miR-124 was significantly down-regulated while CDK4 expression was up-regulated; And the expression of miR-124 and CDK4 showed an obvious inverse correlation in these xenograft tissues which was also observed in human bladder cancer tissue samples; Taken together our results strongly suggest that miR-124 can arrest cell cycle and restrain the growth of bladder cancer by targeting CDK4 directly;MiR 124 inhibits cell proliferation in breast cancer through downregulation of CDK4; We identified and confirmed that cyclin-dependent kinase 4 CDK4 was a direct target of miR-124; Overexpression of miR-124 suppressed CDK4 protein expression and attenuated cell viability proliferation and cell cycle progression in MCF-7 and MDA-MB-435S breast cancer cells in vitro; Overexpression of CDK4 partially rescued the inhibitory effect of miR-124 in the breast cancer cells; Our results demonstrate that miR-124 functions as a growth-suppressive miRNA and plays an important role in inhibiting tumorigenesis by targeting CDK4;miR 124 radiosensitizes human esophageal cancer cell TE 1 by targeting CDK4; Finally we identified that CDK4 is a direct target of miR-124 in TE-1 cells using target prediction algorithms and a luciferase reporter assay; Moreover western blot assay confirmed that CDK4 was downregulated during miR-124 transfection ;progression;tumorigenesis; bladder cancer;breast cancer;esophageal cancer hsa-miR-145-5p CDK4 0.108948399943015 0.310032352776693 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.175782720088333 2.61540642411898e-07 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-24-3p CDK4 0.133257261462871 0.212960921400853 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.144306679535031 6.44290009253847e-05 NA NA NA hsa-miR-34a-5p CDK4 0.143472919835524 0.211283228567057 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate;miRTarBase -0.118448417522733 0.000139347458480003 25789847 Real-time PCR and western blot analysis of extracted RNA and total protein revealed artemsinin and artesunate increased miR-34a expression in a dose-dependent manner correlating with down-regulation of the miR-34a target gene CDK4; Phytochemical treatments inhibited the luciferase activity of a construct containing the wild-type 3'UTR of CDK4 but not those with a mutated miR-34a binding site whereas transfection of miR-34a inhibitors ablated the phytochemical mediated down-regulation of CDK4 and induction of cell cycle arrest breast cancer hsa-miR-766-3p CDK4 -0.147747825439875 0.266658981351507 0.445809779300479 3.53133251325681e-07 miRNAWalker2_validate -0.211336637770052 5.92406894308187e-17 NA NA NA hsa-miR-101-3p CDK6 -0.132333931567244 0.241891922291381 0.514594252893836 5.86679673505214e-06 mirMAP -0.214050983778852 0.000115517087379797 NA NA NA hsa-miR-1179 CDK6 -0.117056451908751 0.310989525634765 0.514594252893836 5.86679673505214e-06 PITA;miRNATAP -0.142889521672303 0.000881663691628466 NA NA NA hsa-miR-1224-5p CDK6 -0.53941068839383 0.00201058335769201 0.514594252893836 5.86679673505214e-06 miRNATAP -0.101570856158794 0.000240685196252074 NA NA NA hsa-miR-129-5p CDK6 -0.567133990117126 0.00116152215679936 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate -0.103420012068894 0.00024955678235129 24055727 Interestingly we showed that cyclin dependent kinase 6 CDK6 a cell cycle-associated protein involved in G1-S transition was a target of miR-129 gastric cancer hsa-miR-137 CDK6 -0.924849266740909 1.546832202049e-06 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.100134233590921 5.13631857844561e-05 18577219;25342326;23178712;21051724 Transfection of microRNA-124 or microRNA-137 also induced G1 cell cycle arrest in U251 and SF6969 glioblastoma multiforme cells which was associated with decreased expression of cyclin-dependent kinase 6 and phosphorylated retinoblastoma pSer 807/811 proteins;Bioinformatics prediction and luciferase reporter assay revealed CDK6 as a target gene through which miR-137 exerted an inhibitory function;miR 137 inhibits the proliferation of lung cancer cells by targeting Cdc42 and Cdk6; Ectopic expression of miR-137 in lung cancer cells significantly downregulated Cdc42 Cdk6 and induced G1 cell cycle arrest leading to a significant decrease in cell growth in vivo and in vitro; Further both Cdc42 and Cdk6 were confirmed as targets of miR-137;The results showed that miR-137 can act as a tumor suppressor in uveal melanoma cell proliferation through downregulation of the targets MITF and CDK6 miR-137 may be epigenetically silenced during uveal melanoma tumorigenesis ;;;tumorigenesis glioblastoma;gastric cancer;lung cancer;melanoma hsa-miR-140-3p CDK6 -0.123864258867705 0.14796686619449 0.514594252893836 5.86679673505214e-06 miRNATAP -0.26390527008378 0.000421888747453862 NA NA NA hsa-miR-29a-3p CDK6 -0.224140851576083 0.0349516103550236 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.295012706425205 5.99888048504157e-07 22493297;23245396;20086245 In addition a target of miR-29a cyclin-dependent kinase 6 gene and a target of miR-142-3p TGF-β-activated kinase 1/MAP3K7 binding protein 2 gene are involved in the regulation of both monocytic and granulocytic differentiation;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL differentiation;; acute myeloid leukemia;melanoma;lymphoma hsa-miR-29b-3p CDK6 -0.178522712044497 0.0856106432165465 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.269230638368595 2.03842569918004e-07 26180082;23245396;25472644;23591808;27230400;20086245 Knockdown of NTSR1 increased the expression of miR-29b-1 and miR-129-3p which were responsible for the decreased CDK6 expression;The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;Moreover miR-29b inhibited the expression of MCL1 and CDK6;Here we have identified the oncogene cyclin-dependent protein kinase 6 CDK6 as a direct target of miR-29b in lung cancer;MiR 29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6; In this study we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods; We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells; Furthermore we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues; The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL ;;;;; glioblastoma;melanoma;colorectal cancer;lung cancer;sarcoma;lymphoma hsa-miR-29c-3p CDK6 -0.372180390387259 0.00202634431973208 0.514594252893836 5.86679673505214e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.208117488470636 2.11482006457337e-06 26396669 Furthermore through qPCR and Western blot assays confirmed that overexpression of miR-29c reduced CDK6 mRNA and protein levels; miR-29c could inhibit the proliferation migration and invasion of bladder cancer cells via regulating CDK6 bladder cancer hsa-miR-30a-5p CDK6 -0.062942936611238 0.513851954700252 0.514594252893836 5.86679673505214e-06 mirMAP -0.216479485176302 0.00926959328929756 NA NA NA hsa-miR-30b-5p CDK6 -0.0858101016322106 0.263243418521854 0.514594252893836 5.86679673505214e-06 mirMAP -0.264336978353372 0.000515451713470756 NA NA NA hsa-miR-30d-3p CDK6 0.0854049347792898 0.20691254801121 0.514594252893836 5.86679673505214e-06 mirMAP -0.200436058332701 0.00560114486149501 NA NA NA hsa-miR-30d-5p CDK6 -0.0607010868720046 0.471410043548794 0.514594252893836 5.86679673505214e-06 mirMAP -0.300718358106314 0.000558788158739809 NA NA NA hsa-miR-338-3p CDK6 -0.39963704679384 0.0034410643220691 0.514594252893836 5.86679673505214e-06 mirMAP -0.123780088353193 0.00144999386822314 NA NA NA hsa-miR-362-5p CDK6 -0.0730185557229279 0.425572997292224 0.514594252893836 5.86679673505214e-06 mirMAP -0.16543927930901 0.0021247110211983 NA NA NA hsa-miR-488-5p CDK6 -0.385755602369049 7.99431673275965e-08 0.514594252893836 5.86679673505214e-06 mirMAP -0.265214262784328 0.000150929371106913 NA NA NA hsa-miR-491-3p CDK6 -0.523374665885358 1.93479565404951e-05 0.514594252893836 5.86679673505214e-06 PITA;miRNATAP -0.14151833133198 0.000308714771991301 NA NA NA hsa-miR-660-5p CDK6 -0.138989893523975 0.0945491063873448 0.514594252893836 5.86679673505214e-06 mirMAP -0.206589176560963 0.00107106139495866 NA NA NA hsa-miR-7-1-3p CDK6 -0.0894475316162393 0.37322359785108 0.514594252893836 5.86679673505214e-06 mirMAP -0.161897757484807 0.000856176984301135 NA NA NA hsa-miR-767-5p CDK6 -0.551653504513145 2.61109058244854e-05 0.514594252893836 5.86679673505214e-06 mirMAP -0.218990178100663 3.12583485290879e-09 NA NA NA hsa-miR-885-5p CDK6 -0.423614695846868 1.14213289528402e-05 0.514594252893836 5.86679673505214e-06 mirMAP -0.261342311783077 2.73713377344836e-07 NA NA NA hsa-miR-935 CDK6 -0.277848050075718 0.02166231531395 0.514594252893836 5.86679673505214e-06 mirMAP -0.11716238994515 0.00413414436299781 NA NA NA hsa-let-7c-5p CDKN1A -0.04295851886768 0.676052842748691 0.427121917792729 0.00123136007948734 MirTarget -0.323059985687549 1.83340518666355e-05 NA NA NA hsa-let-7i-5p CDKN1A 0.0952853587923244 0.192120688957249 0.427121917792729 0.00123136007948734 MirTarget -0.290880872793201 0.00310217886465504 NA NA NA hsa-miR-106b-5p CDKN1A 0.167096183547615 0.0497544396383451 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.354916178485011 1.93698403552949e-06 NA NA NA hsa-miR-125a-5p CDKN1A -0.0833349424517387 0.325440200253607 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase -0.417635698065873 2.90152784450923e-07 NA NA NA hsa-miR-129-2-3p CDKN1A -0.6175132197013 0.000561573620904867 0.427121917792729 0.00123136007948734 mirMAP -0.132493112138164 2.17805696270136e-05 NA NA NA hsa-miR-17-5p CDKN1A 0.0975751369167082 0.20621110214601 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.455125254344935 4.24790487714932e-07 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-455-3p CDKN1A 0.512701816402104 0.000459755983280155 0.427121917792729 0.00123136007948734 MirTarget -0.108213069650159 0.00527030056074479 NA NA NA hsa-miR-491-5p CDKN1A -0.749881113074983 8.22859263179194e-09 0.427121917792729 0.00123136007948734 miRanda -0.187701364863274 8.68850198681925e-06 NA NA NA hsa-miR-505-5p CDKN1A 0.0973008785018434 0.183209581426791 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;MirTarget -0.381395926281218 9.16666243828523e-07 NA NA NA hsa-miR-708-5p CDKN1A -0.00936519718301376 0.919857671524527 0.427121917792729 0.00123136007948734 MirTarget -0.340713510326019 2.02954343368862e-07 NA NA NA hsa-miR-93-5p CDKN1A 0.139766237971122 0.101057320076629 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.407487489770916 6.20600753969256e-05 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-98-5p CDKN1A -0.0806971683871618 0.251777941211683 0.427121917792729 0.00123136007948734 miRNAWalker2_validate;MirTarget -0.306627890186986 0.000734149322136236 NA NA NA hsa-miR-148a-3p CDKN1B 0.306821592493439 0.0392356958885949 -0.0903104874713394 0.0907488975491606 miRNATAP -0.104087004262967 7.76609987083251e-22 NA NA NA hsa-miR-221-3p CDKN1B -0.0109507285071775 0.936440693394519 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.110743557621931 6.97784252871238e-23 19953484;17721077;23637992;23939688;19126397;20146005;23967190;17569667;22992757;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;progression;;;staging;tumorigenesis;;;;poor survival glioblastoma;glioblastoma;breast cancer;breast cancer;melanoma;liver cancer;pancreatic cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-222-3p CDKN1B 0.107117065575197 0.423818119725697 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.105894357261447 1.7642291217018e-20 19953484;26912358;24895988;24137356;17569667;27282281;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Besides microvesicle marker characterization we evidenced that miR-222 exosomal expression mostly reflected its abundance in the cells of origin correctly paralleled by repression of its target genes such as p27Kip1 and induction of the PI3K/AKT pathway thus confirming its functional implication in cancer;MiR-222 plays an important role in the tumorigenesis of CC possibly by specifically down-regulating p27Kip1 and PTEN;miR 222 is upregulated in epithelial ovarian cancer and promotes cell proliferation by downregulating P27kip1; miR-222 upregulation induced an enhancement of ovarian cancer cell proliferation potential possibly by downregulating its target P27Kip1; A bioinformatic analysis showed that the 3'-UTR of the P27Kip1 mRNA contained a highly-conserved putative miR-222 binding site; Luciferase reporter assays demonstrated that P27Kip1 was a direct target of miR-222; Consistently there was an inverse correlation between the P27Kip1 and miR-222 expression levels in the ovarian cancer cell lines and tissues;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;miR 222 confers the resistance of breast cancer cells to Adriamycin through suppression of p27kip1 expression; Immunofluorescence showed that miR-222 altered the subcellular location of p27kip1 in nucleus; The results showed that downregulation of miR-222 in MCF-7/Adr increased sensitivity to Adr and Adr-induced apoptosis and arrested the cells in G1 phase accompanied by more expressions of p27kip1 especially in nucleus; Taken together the results found that miR-222 induced Adr-resistance at least in part via suppressing p27kip1 expression and altering its subcellular localization and miR-222 inhibitors could reverse Adr-resistance of breast cancer cells;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;tumorigenesis;;;drug resistance;poor survival glioblastoma;melanoma;cervical and endocervical cancer;ovarian cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-24-3p CDKN1B 0.133257261462871 0.212960921400853 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate;miRNATAP -0.113888072566706 2.15931284116347e-12 26847530;26044523 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated;With the bioinformatic method we further identified that p27Kip1 is a direct target of miR-24-3p and its protein level was negatively regulated by miR-24-3p ; prostate cancer;breast cancer hsa-miR-30a-3p CDKN1B 0.0655410309550977 0.509541256241954 -0.0903104874713394 0.0907488975491606 MirTarget -0.135932822746313 3.76783512403037e-11 NA NA NA hsa-miR-30e-3p CDKN1B 0.00173769765874887 0.984965501547409 -0.0903104874713394 0.0907488975491606 MirTarget -0.126819201706031 5.97208515819098e-08 NA NA NA hsa-miR-455-5p CDKN1B 0.332224239762 0.0106491358056238 -0.0903104874713394 0.0907488975491606 miRanda;miRNATAP -0.103724305413802 2.1331574276816e-18 NA NA NA hsa-miR-98-5p CDKN1B -0.0806971683871618 0.251777941211683 -0.0903104874713394 0.0907488975491606 miRNAWalker2_validate -0.161056976459826 1.93301457144278e-10 NA NA NA hsa-let-7g-5p CDKN2A -0.0253396334608382 0.742556489443668 0.0187886573712563 0.903922340142693 miRNAWalker2_validate;miRTarBase -0.34824822585108 0.00265574549691866 NA NA NA hsa-miR-124-3p CDKN2A -0.506026514549291 0.0078056370358648 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.250164816877691 1.31566252867206e-12 NA NA NA hsa-miR-125a-5p CDKN2A -0.0833349424517387 0.325440200253607 0.0187886573712563 0.903922340142693 miRanda -0.370077543367592 0.000189260563152586 NA NA NA hsa-miR-128-3p CDKN2A -0.781932174412672 7.1812756321342e-10 0.0187886573712563 0.903922340142693 MirTarget -0.262360804552711 6.19986805514199e-06 NA NA NA hsa-miR-155-5p CDKN2A 0.969837380981085 5.86551495710056e-15 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.18750964292148 0.000446932476411726 NA NA NA hsa-miR-16-5p CDKN2A 0.246024668671282 0.00346042804503716 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.399970122623873 2.77212055657459e-05 NA NA NA hsa-miR-192-5p CDKN2A -0.0426540615456616 0.621616927627554 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.355895513978692 0.000105711706086703 NA NA NA hsa-miR-24-3p CDKN2A 0.133257261462871 0.212960921400853 0.0187886573712563 0.903922340142693 miRNAWalker2_validate;miRTarBase -0.286694703652825 4.87185115774767e-05 26847530;22336108 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated; p27 and p16 were confirmed as targets of miR-24 in prostate cancer cells and a significant inverse correlation between miR-24 and p27 was revealed in clinical prostatectomy specimens; These findings provide evidence that miR-24 has a tumor suppressor role in prostate cancer and also targets p27 and p16 in prostate cancer cells;To study p14ARF biogenesis retinoblastoma cells were treated with the proteasome inhibitor MG132 and siRNA against miR-24; miR-24 a microRNA that represses p14ARF expression is expressed in retinoblastoma cell lines and correlates with lower protein expression when compared to other cell lines with high p14ARF mRNA; Transient over-expression of siRNA against miR-24 led to elevated p14ARF protein in retinoblastoma cells; p14ARF protein levels were restored without change in mRNA abundance upon miR-24 inhibition suggesting that miR-24 could functionally repress expression effectively blocking p53 tumor surveillance ; prostate cancer;retinoblastoma hsa-miR-34a-5p CDKN2A 0.143472919835524 0.211283228567057 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.275930017996842 5.30358193376552e-06 25862914;26734589 MiR-34a tumor levels significantly correlated with oropharyngeal origin p=0.0284 and p16 positivity p=0.0218;The Significance of miR 34a Expression in Endometrial Carcinogenesis: Correlation With Expression of p16 and Ki 67 Proteins in Endometrial Cancers; This study was undertaken to analyze miR-34a expression in simple endometrial hyperplasia and endometrial cancer and to evaluate the relationship between expression of miR-34a and p16 and Ki-67 proteins in endometrial cancers; These were analyzed for miR-34a expression by quantitative real-time PCR and the expression of p16 and Ki-67 proteins in endometrial cancers was evaluated by immunohistochemistry; Although not statistically significant the frequency of p16 and Ki-67 overexpression tended to be lower in the cases with miR-34a underexpression than in cases with miR-34a overexpression ;tumorigenesis head and neck cancer;endometrial cancer hsa-miR-365a-3p CDKN2A -0.184222766207919 0.0292663235724171 0.0187886573712563 0.903922340142693 MirTarget -0.214641593907008 0.00965027186755129 NA NA NA hsa-miR-455-3p CDKN2A 0.512701816402104 0.000459755983280155 0.0187886573712563 0.903922340142693 miRNAWalker2_validate -0.168237751538781 0.000328290439616957 NA NA NA hsa-miR-671-5p CDKN2A 0.379677704170736 0.000129391032677459 0.0187886573712563 0.903922340142693 PITA -0.335505749888256 7.04085752831714e-07 NA NA NA hsa-miR-940 CDKN2A -0.0183807673806575 0.881747775878136 0.0187886573712563 0.903922340142693 MirTarget -0.411718461242004 1.98167555161455e-14 NA NA NA hsa-miR-107 CDKN2B -0.121070851008387 0.198381281894242 -0.254500650033998 0.0580107490431447 PITA -0.36493021023524 1.72102029453711e-08 NA NA NA hsa-miR-125a-3p CDKN2B 0.183834985432376 0.106230647107917 -0.254500650033998 0.0580107490431447 miRanda -0.212208871166161 3.69511896753134e-05 NA NA NA hsa-miR-126-5p CDKN2B 0.177332403948664 0.0711675116243123 -0.254500650033998 0.0580107490431447 mirMAP -0.385392085012657 2.72330784929511e-10 NA NA NA hsa-miR-137 CDKN2B -0.924849266740909 1.546832202049e-06 -0.254500650033998 0.0580107490431447 miRanda -0.181124795647527 6.72800234722017e-10 NA NA NA hsa-miR-144-3p CDKN2B 0.185426471537742 0.329598425352088 -0.254500650033998 0.0580107490431447 MirTarget -0.112030428392251 0.00026905020498593 NA NA NA hsa-miR-330-3p CDKN2B -0.427388106938151 0.00300489284414266 -0.254500650033998 0.0580107490431447 mirMAP;miRNATAP -0.170911889979298 1.82835015783354e-05 NA NA NA hsa-miR-335-3p CDKN2B 0.34540584335595 0.00043848660222294 -0.254500650033998 0.0580107490431447 mirMAP -0.417444316508558 3.74804764811576e-12 NA NA NA hsa-miR-340-5p CDKN2B -0.110504550202736 0.20522426193476 -0.254500650033998 0.0580107490431447 mirMAP -0.214660759080386 0.00250781933908224 NA NA NA hsa-miR-375 CDKN2B -0.0268401141324031 0.858358101444697 -0.254500650033998 0.0580107490431447 miRNAWalker2_validate;miRNATAP -0.148102003290624 0.00011060223809831 NA NA NA hsa-miR-382-5p CDKN2B -0.169491096802872 0.231437468967178 -0.254500650033998 0.0580107490431447 mirMAP -0.231264423148089 1.31932072204718e-08 NA NA NA hsa-miR-450b-5p CDKN2B 0.270544879013992 0.00801537838499562 -0.254500650033998 0.0580107490431447 mirMAP -0.174359721848087 0.00254948464423664 NA NA NA hsa-miR-451a CDKN2B -0.0440943252378805 0.79823866173551 -0.254500650033998 0.0580107490431447 MirTarget -0.124674442269203 0.000304938611921899 NA NA NA hsa-miR-486-5p CDKN2B -0.121638590818052 0.446088157530731 -0.254500650033998 0.0580107490431447 miRanda -0.107363771300245 0.00366340014949506 NA NA NA hsa-miR-495-3p CDKN2B -0.421982588962504 0.00504424038526901 -0.254500650033998 0.0580107490431447 MirTarget -0.193686846998077 4.3661816950633e-07 NA NA NA hsa-miR-582-5p CDKN2B -0.215764335500665 0.0760892640434509 -0.254500650033998 0.0580107490431447 miRNATAP -0.316332165128154 1.0917579180994e-11 NA NA NA hsa-miR-671-5p CDKN2B 0.379677704170736 0.000129391032677459 -0.254500650033998 0.0580107490431447 PITA -0.277663565525736 1.44477119747515e-06 NA NA NA hsa-miR-7-1-3p CDKN2B -0.0894475316162393 0.37322359785108 -0.254500650033998 0.0580107490431447 MirTarget -0.217907904533878 0.000178196185959534 NA NA NA hsa-miR-98-5p CDKN2B -0.0806971683871618 0.251777941211683 -0.254500650033998 0.0580107490431447 miRNAWalker2_validate -0.258385050457077 0.00585538645882101 NA NA NA hsa-miR-129-5p CDKN2C -0.567133990117126 0.00116152215679936 0.898221520592176 6.4070286196519e-16 miRanda -0.186505554250998 3.78408526778196e-11 NA NA NA hsa-miR-491-3p CDKN2C -0.523374665885358 1.93479565404951e-05 0.898221520592176 6.4070286196519e-16 miRNATAP -0.20754973694785 1.45748829977249e-07 NA NA NA hsa-miR-577 CDKN2C -0.382068371859275 0.0145513192729119 0.898221520592176 6.4070286196519e-16 MirTarget;PITA;miRNATAP -0.115189053156569 0.000263913273443431 NA NA NA hsa-miR-107 CHEK1 -0.121070851008387 0.198381281894242 0.573955749984589 8.28992689476689e-16 miRanda;miRNATAP -0.114487588080244 0.00132462804560562 NA NA NA hsa-miR-129-5p CHEK1 -0.567133990117126 0.00116152215679936 0.573955749984589 8.28992689476689e-16 miRanda -0.122309084327909 1.74565500015445e-11 NA NA NA hsa-miR-139-5p CHEK1 -0.647754756897633 2.15378307237163e-06 0.573955749984589 8.28992689476689e-16 miRanda -0.187266116631356 7.28244952419277e-16 NA NA NA hsa-miR-326 CHEK1 -0.631050656251074 8.83643450601471e-05 0.573955749984589 8.28992689476689e-16 miRanda -0.127671135956966 2.62556784627874e-11 NA NA NA hsa-miR-330-5p CHEK1 -0.262932095995605 0.0465226565966658 0.573955749984589 8.28992689476689e-16 miRanda -0.118282510893023 6.77322870635937e-07 NA NA NA hsa-miR-346 CHEK1 -0.577786425963268 2.10883270131593e-09 0.573955749984589 8.28992689476689e-16 miRanda -0.261286859784501 1.56543047407142e-16 NA NA NA hsa-miR-491-3p CHEK1 -0.523374665885358 1.93479565404951e-05 0.573955749984589 8.28992689476689e-16 MirTarget -0.175954445428317 2.99887467961377e-12 NA NA NA hsa-miR-99b-5p CHEK1 0.0938366784257259 0.419406306408393 0.573955749984589 8.28992689476689e-16 miRNAWalker2_validate -0.112056772233989 0.00170409121443777 NA NA NA hsa-let-7e-5p CREBBP 0.0471189209570344 0.657255455444655 -0.047978601693571 0.404366818323115 miRNAWalker2_validate -0.117826371466181 2.40828040481683e-09 NA NA NA hsa-miR-1301-3p CREBBP -0.105695714210758 0.152249184194132 -0.047978601693571 0.404366818323115 miRNAWalker2_validate -0.145041633909724 7.95662975669671e-09 NA NA NA hsa-miR-142-5p CREBBP 0.434884875454107 0.000575570345702788 -0.047978601693571 0.404366818323115 mirMAP -0.106025113337559 1.85000672571322e-14 NA NA NA hsa-miR-186-5p CREBBP 0.0606381894130505 0.514217717592464 -0.047978601693571 0.404366818323115 mirMAP;miRNATAP -0.147890785462563 1.79437968717686e-12 NA NA NA hsa-miR-200b-3p CREBBP 0.245768526504511 0.0580744465744737 -0.047978601693571 0.404366818323115 mirMAP -0.123048999824309 2.44260177280472e-19 NA NA NA hsa-miR-200c-3p CREBBP 0.413752622631695 8.6936333715621e-06 -0.047978601693571 0.404366818323115 mirMAP -0.133825633175237 1.58816104033339e-12 NA NA NA hsa-miR-26a-5p CREBBP 0.033823776747294 0.726610414342369 -0.047978601693571 0.404366818323115 miRNATAP -0.116054995750247 2.26207553528174e-07 NA NA NA hsa-miR-26b-5p CREBBP -0.0984536538762768 0.231803769574623 -0.047978601693571 0.404366818323115 miRNATAP -0.164373734995276 2.00260444141814e-10 NA NA NA hsa-miR-30a-3p CREBBP 0.0655410309550977 0.509541256241954 -0.047978601693571 0.404366818323115 MirTarget;miRNATAP -0.118406533193908 4.53827599405547e-07 NA NA NA hsa-miR-30e-3p CREBBP 0.00173769765874887 0.984965501547409 -0.047978601693571 0.404366818323115 MirTarget -0.140461173361411 1.16147592773995e-07 NA NA NA hsa-miR-361-5p CREBBP 0.0107519600643302 0.882230614095565 -0.047978601693571 0.404366818323115 PITA;miRanda;miRNATAP -0.20977112720493 4.42799460550161e-12 NA NA NA hsa-miR-374a-3p CREBBP 0.176226170437625 0.0343958848425446 -0.047978601693571 0.404366818323115 miRNATAP -0.166006934673094 4.57407449473457e-10 NA NA NA hsa-miR-548b-3p CREBBP 0.340474544210108 0.00224491592019745 -0.047978601693571 0.404366818323115 miRNATAP -0.122339265277455 1.92040117401605e-14 NA NA NA hsa-miR-590-3p CREBBP 0.312951515438396 0.000480455423843996 -0.047978601693571 0.404366818323115 PITA;miRanda;mirMAP;miRNATAP -0.145167352998136 1.52352483162506e-13 NA NA NA hsa-miR-628-3p CREBBP -0.243661425878074 0.0594758496279051 -0.047978601693571 0.404366818323115 PITA -0.103726817505493 6.28819709328743e-14 NA NA NA hsa-miR-3065-3p DBF4 -0.261348288537884 0.0343753235107924 0.431630573018507 4.49252182853658e-11 MirTarget -0.115036064325221 1.39376625464602e-06 NA NA NA hsa-miR-30a-5p DBF4 -0.062942936611238 0.513851954700252 0.431630573018507 4.49252182853658e-11 MirTarget -0.454370129509175 2.09947562567316e-22 NA NA NA hsa-miR-30b-5p DBF4 -0.0858101016322106 0.263243418521854 0.431630573018507 4.49252182853658e-11 MirTarget -0.22291903459348 4.61926372634965e-07 NA NA NA hsa-miR-30c-5p DBF4 -0.0469114469943097 0.565934557464627 0.431630573018507 4.49252182853658e-11 MirTarget -0.245137374108006 3.10513913748762e-08 NA NA NA hsa-miR-30d-5p DBF4 -0.0607010868720046 0.471410043548794 0.431630573018507 4.49252182853658e-11 MirTarget -0.324544149111564 1.03594127601206e-10 NA NA NA hsa-miR-30e-5p DBF4 0.0764521828640934 0.41904991629622 0.431630573018507 4.49252182853658e-11 MirTarget -0.210104881166212 5.32483759016847e-07 NA NA NA hsa-miR-330-3p DBF4 -0.427388106938151 0.00300489284414266 0.431630573018507 4.49252182853658e-11 MirTarget -0.147632454365299 9.83878074315976e-15 NA NA NA hsa-miR-342-3p DBF4 -0.125683829770877 0.105810751640137 0.431630573018507 4.49252182853658e-11 miRanda -0.13872066195901 0.000687824150801523 NA NA NA hsa-miR-98-5p DBF4 -0.0806971683871618 0.251777941211683 0.431630573018507 4.49252182853658e-11 miRNAWalker2_validate -0.23118026282996 3.37336295796131e-07 NA NA NA hsa-let-7e-5p E2F2 0.0471189209570344 0.657255455444655 1.34827244761579 5.91940938359519e-17 MirTarget -0.227577637619613 0.00488223035838476 NA NA NA hsa-miR-125a-5p E2F2 -0.0833349424517387 0.325440200253607 1.34827244761579 5.91940938359519e-17 MirTarget -0.461569157612989 1.22786103450127e-05 NA NA NA hsa-miR-185-3p E2F2 -0.133219014400353 0.255713818728239 1.34827244761579 5.91940938359519e-17 MirTarget -0.221464640291927 0.000349011855987302 NA NA NA hsa-miR-193b-3p E2F2 0.0766333116224072 0.324723824453143 1.34827244761579 5.91940938359519e-17 miRNAWalker2_validate -0.38514640995334 8.96744661984587e-05 NA NA NA hsa-miR-204-5p E2F2 -0.288588537398065 0.113371226435822 1.34827244761579 5.91940938359519e-17 mirMAP -0.167962539797751 2.56052343338108e-05 NA NA NA hsa-miR-22-3p E2F2 0.0277287593466351 0.809325106832648 1.34827244761579 5.91940938359519e-17 miRNAWalker2_validate -0.593831337538519 5.84089457404813e-12 NA NA NA hsa-miR-26a-5p E2F2 0.033823776747294 0.726610414342369 1.34827244761579 5.91940938359519e-17 miRNATAP -0.238946953225521 0.00899757878295909 26458859 MiR 26a enhances the sensitivity of gastric cancer cells to cisplatin by targeting NRAS and E2F2; Our results suggest that miR-26a can improve the sensitivity of GC cells to cisplatin-based chemotherapies through targeting NRAS and E2F2 and provide the first evidence of the potential utility of miR-26a as a sensitizer in chemotherapy for GC gastric cancer hsa-miR-26b-5p E2F2 -0.0984536538762768 0.231803769574623 1.34827244761579 5.91940938359519e-17 miRNATAP -0.279929616122037 0.00833489565893885 NA NA NA hsa-miR-30c-2-3p E2F2 -0.18630560418806 0.0313113706136533 1.34827244761579 5.91940938359519e-17 MirTarget -0.531057578005903 4.73180385011406e-09 NA NA NA hsa-miR-326 E2F2 -0.631050656251074 8.83643450601471e-05 1.34827244761579 5.91940938359519e-17 MirTarget -0.280624262853395 2.00326576109166e-10 NA NA NA hsa-miR-330-5p E2F2 -0.262932095995605 0.0465226565966658 1.34827244761579 5.91940938359519e-17 MirTarget;miRNATAP -0.346942874107683 1.70115694039137e-10 NA NA NA hsa-miR-331-3p E2F2 0.0126984726684189 0.905175053956974 1.34827244761579 5.91940938359519e-17 miRNAWalker2_validate -0.22862799370917 0.000950309250903931 NA NA NA hsa-miR-338-3p E2F2 -0.39963704679384 0.0034410643220691 1.34827244761579 5.91940938359519e-17 mirMAP -0.33969687934866 4.05953795332898e-09 NA NA NA hsa-miR-365a-3p E2F2 -0.184222766207919 0.0292663235724171 1.34827244761579 5.91940938359519e-17 MirTarget -0.455918727958444 1.98971122282273e-07 NA NA NA hsa-miR-378c E2F2 0.169076160997048 0.114131854194064 1.34827244761579 5.91940938359519e-17 MirTarget -0.292046160835513 2.37761097277519e-05 NA NA NA hsa-miR-432-5p E2F2 -0.214242438141977 0.166186087759946 1.34827244761579 5.91940938359519e-17 mirMAP -0.169394779115155 0.0002959313864057 NA NA NA hsa-miR-488-3p E2F2 -0.257955761012568 0.00280795162806256 1.34827244761579 5.91940938359519e-17 mirMAP -0.500491651618079 4.21227626758742e-09 NA NA NA hsa-miR-490-3p E2F2 -0.834268051506884 5.26203010743215e-06 1.34827244761579 5.91940938359519e-17 mirMAP -0.262096603370546 2.67497437024177e-11 NA NA NA hsa-miR-496 E2F2 -0.139833482232877 0.39597541862532 1.34827244761579 5.91940938359519e-17 miRanda -0.176222529328534 7.85797264595003e-05 NA NA NA hsa-miR-539-5p E2F2 -0.326485413953598 0.0554453076411246 1.34827244761579 5.91940938359519e-17 mirMAP -0.212027643333352 5.03744244110982e-07 NA NA NA hsa-miR-625-5p E2F2 -0.0264137834407145 0.802098211251158 1.34827244761579 5.91940938359519e-17 mirMAP -0.217792841278429 0.00170029040977415 NA NA NA hsa-miR-628-5p E2F2 -0.0469793532265896 0.657420003383685 1.34827244761579 5.91940938359519e-17 mirMAP -0.317783864402383 5.36506329866132e-06 NA NA NA hsa-miR-7-5p E2F2 -0.244254069762734 0.193173919522103 1.34827244761579 5.91940938359519e-17 miRNAWalker2_validate -0.154333499014073 8.78572177240792e-05 NA NA NA hsa-miR-766-3p E2F2 -0.147747825439875 0.266658981351507 1.34827244761579 5.91940938359519e-17 mirMAP -0.359513756029547 1.55793767609969e-11 NA NA NA hsa-miR-125a-5p E2F3 -0.0833349424517387 0.325440200253607 0.27471125898991 1.57127947268041e-05 PITA;miRanda;miRNATAP -0.181828836316357 5.57346715453653e-07 NA NA NA hsa-miR-140-3p E2F3 -0.123864258867705 0.14796686619449 0.27471125898991 1.57127947268041e-05 mirMAP -0.106266714726071 0.00632350228312719 NA NA NA hsa-miR-30a-5p E2F3 -0.062942936611238 0.513851954700252 0.27471125898991 1.57127947268041e-05 miRNATAP -0.411126053341067 2.59564083751805e-23 NA NA NA hsa-miR-30b-5p E2F3 -0.0858101016322106 0.263243418521854 0.27471125898991 1.57127947268041e-05 miRNATAP -0.139178339753518 0.000423125230818794 NA NA NA hsa-miR-30c-5p E2F3 -0.0469114469943097 0.565934557464627 0.27471125898991 1.57127947268041e-05 miRNAWalker2_validate;miRNATAP -0.216271867425936 3.74811317343565e-08 NA NA NA hsa-miR-320a E2F3 0.092135599636725 0.243028758989929 0.27471125898991 1.57127947268041e-05 PITA;miRanda;miRNATAP -0.127770575118655 0.00134574568688681 NA NA NA hsa-miR-34a-5p E2F3 0.143472919835524 0.211283228567057 0.27471125898991 1.57127947268041e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.148016505602695 2.00146004442685e-11 21067862;23954321;22629428;25675046;26518892 The ectopic expression of miR-34a in the 5-FU-resistant cells inhibited growth as in the parental cells and attenuated the resistance to 5-FU through the down-regulation of Sirt1 and E2F3; These findings suggest that miR-34a targeting the Sirt1 and E2F3 genes could negatively regulate at least in part the resistance to 5-FU in human colorectal cancer DLD-1 cells;Interestingly RES increased the intracellular expression level of miR-34a which down-regulated the target gene E2F3 and its downstream Sirt1 resulting in growth inhibition;miR-34a overexpression also markedly downregulated E2F3 and survivin levels; In addition tumor samples from HNSCC patients showed an inverse relationship between miR-34a and survivin as well as miR-34a and E2F3 levels;MiR 34a Inhibits Viability and Invasion of Human Papillomavirus Positive Cervical Cancer Cells by Targeting E2F3 and Regulating Survivin; This study explored the regulative role of miR-34a on E2F3 and survivin expression and the viability and invasion of HPV-positive cervical cancer cells; E2F3 is a direct target of miR-34a in HPV-positive cervical cancer cells; By targeting E2F3 miR-34a could regulate the expression of survivin; Thus through regulating E2F3 and survivin miR-34a could reduce the viability and invasion of HPV-positive cervical cancer cells;For example overexpressing miR-34a a master regulator of tumor suppression attenuates chemoresistance to 5-FU by downregulating silent information regulator 1 SIRT1 and E2F3 drug resistance;;;;drug resistance colon cancer;colon cancer;head and neck cancer;cervical and endocervical cancer;colorectal cancer hsa-miR-500a-3p E2F3 0.0479457078972052 0.49420307623914 0.27471125898991 1.57127947268041e-05 miRNATAP -0.205460559175235 9.4101897482497e-07 NA NA NA hsa-miR-501-5p E2F3 0.0878951859817452 0.309663377222443 0.27471125898991 1.57127947268041e-05 PITA -0.108163924260959 0.000205027521375213 NA NA NA hsa-miR-532-5p E2F3 0.0263466708637097 0.75763006039876 0.27471125898991 1.57127947268041e-05 mirMAP -0.146280923664412 4.4684107014024e-05 NA NA NA hsa-let-7d-5p E2F5 0.111345229817909 0.0872634398336815 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.428797563942653 6.45391488433833e-21 NA NA NA hsa-let-7e-5p E2F5 0.0471189209570344 0.657255455444655 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.201750031886798 7.7559686443878e-14 NA NA NA hsa-let-7f-5p E2F5 0.0508269185845673 0.62677515045793 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.108847620847748 0.000528510794902153 NA NA NA hsa-let-7g-3p E2F5 0.00917503654783935 0.92786975338911 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.138409842665498 3.69889147763859e-08 NA NA NA hsa-let-7g-5p E2F5 -0.0253396334608382 0.742556489443668 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.287678561056155 3.99740690624173e-12 NA NA NA hsa-let-7i-5p E2F5 0.0952853587923244 0.192120688957249 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.126939995855578 0.00344960575102825 NA NA NA hsa-miR-1-3p E2F5 -0.218035884810576 0.125196862509753 0.0588682197727923 0.330185668403852 MirTarget -0.140224292307629 9.44277709496179e-17 NA NA NA hsa-miR-106a-5p E2F5 0.110074064773897 0.13045815990621 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.160629488028761 8.33414166283453e-06 NA NA NA hsa-miR-124-3p E2F5 -0.506026514549291 0.0078056370358648 0.0588682197727923 0.330185668403852 miRNAWalker2_validate;MirTarget;miRNATAP -0.116337144209906 3.75933641277741e-20 NA NA NA hsa-miR-1271-5p E2F5 0.101224150198862 0.215169362708611 0.0588682197727923 0.330185668403852 MirTarget -0.113618376862507 0.000236666809312955 NA NA NA hsa-miR-132-3p E2F5 -0.178379362833856 0.13335200877129 0.0588682197727923 0.330185668403852 MirTarget -0.209409964940197 6.98778643183418e-23 27186275 miR 132 targeting E2F5 suppresses cell proliferation invasion migration in ovarian cancer cells; Mechanism investigation revealed that miR-132 inhibited the expression of transcription factor E2F5 by specifically targeting its mRNA 3'UTR; Moreover the expression level of E2F5 was significantly increased in ovarian cancer tissues than in the adjacent normal tissues and its expression was inversely correlated with miR-132 expression in clinical ovarian cancer tissues; Additionally silencing E2F5 was able to inhibit the proliferation colony formation migration and invasion of ovarian cancer cells parallel to the effect of miR-132 overexpression on the ovarian cancer cells; Meanwhile overexpression of E2F5 reversed the inhibition effect mediated by miR-132 overexpression; These results indicate that miR-132 suppresses the cell proliferation invasion migration in ovarian cancer cells by targeting E2F5 ovarian cancer hsa-miR-154-5p E2F5 -0.226468848904462 0.107380908211261 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.155332998406713 1.00903641414486e-19 27398145;27074041 MicroRNA 154 inhibits growth and invasion of breast cancer cells through targeting E2F5; Luciferase reporter assay and Western blot was used to verify E2F transcription factor 5 protein E2F5 as a novel target gene of miR-154; E2F5 was identified as a target of miR-154 and its expression was inversely correlated with miR-154 expression in clinical breast cancer tissues; In addition downregulation of E2F5 in MCF7 cells had similar effect on cell proliferation cycle migration and invasion by miR-154 induced; These findings indicate that miR-154 acts as a tumor suppressor by targeting E2F5 suggesting miR-154 as a potential therapeutic target for the treatment of breast cancer;The effects of forced miR-154-5p expression or E2F transcription factor 5 E2F5 knockdown on PCa cells were evaluated by cell proliferation flow cytometry cell migration and invasion assays as well as by Western blot analysis; The forced expression of miR-154-5p or E2F5 knockdown significantly restrained cell growth as well as the migratory and invasive capabilities; Hence E2F5 is a direct target gene of miR-154-5p miR-154-5p may play an important role as an inhibitor of proliferation migration and invasion of PCa by targeting E2F5 in PCa cell lines ;cell migration breast cancer;prostate cancer hsa-miR-181a-5p E2F5 -0.178215604431845 0.0700010802255601 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.11625896339486 0.00078747680827904 24529171 Mechanism investigation revealed that miR-181a inhibited the expression of transcription factor E2F5 by specifically targeting its mRNA 3'UTR; Moreover E2F5 inhibition induced cell growth and rescued the suppressive effect of miR-181a inhibitor on the proliferation of SMMC-7721 cells; Those results strongly suggested that HBV down-regulated E2F5 expression in part by up-regulating the expression of miR-181a; Up-regulation of miR-181a by HBV in hepatoma cells may contribute to the progression of HCC possibly by targeting E2F5 suggesting miR-181a plays important role in HCC development progression liver cancer hsa-miR-181b-5p E2F5 0.0260899284211646 0.768941836912518 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.134622452481229 0.000117065216614048 NA NA NA hsa-miR-192-5p E2F5 -0.0426540615456616 0.621616927627554 0.0588682197727923 0.330185668403852 miRNAWalker2_validate -0.121450391800936 0.000287330906947408 NA NA NA hsa-miR-199a-5p E2F5 0.174889610847968 0.0900027418912454 0.0588682197727923 0.330185668403852 miRanda -0.140181735534642 6.49164663009556e-09 NA NA NA hsa-miR-20b-5p E2F5 0.144541062989476 0.0992042333556543 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.114145504313227 0.000183699082275093 NA NA NA hsa-miR-212-3p E2F5 -0.014112941209349 0.918360379512974 0.0588682197727923 0.330185668403852 MirTarget -0.169151564947676 1.74266123683023e-22 NA NA NA hsa-miR-337-3p E2F5 0.117985828329978 0.346392018956732 0.0588682197727923 0.330185668403852 MirTarget;PITA -0.126967490677138 8.97869414127353e-11 NA NA NA hsa-miR-33a-3p E2F5 -0.158366376619191 0.0991512910387226 0.0588682197727923 0.330185668403852 MirTarget;miRNATAP -0.133222054271566 2.45193643120567e-07 NA NA NA hsa-miR-346 E2F5 -0.577786425963268 2.10883270131593e-09 0.0588682197727923 0.330185668403852 miRanda -0.1516851619227 1.85370709704077e-09 NA NA NA hsa-miR-34a-5p E2F5 0.143472919835524 0.211283228567057 0.0588682197727923 0.330185668403852 miRNAWalker2_validate;MirTarget;miRNATAP -0.211020229443497 4.0109348322791e-23 26103003 MicroRNA 34a targets FMNL2 and E2F5 and suppresses the progression of colorectal cancer; FMNL2 and E2F5 were identified as direct targets of miR-34a; Reintroduction of FMNL2 or E2F5 without 3'UTR region reversed the inhibitory effects of miR-34a on cell proliferation and invasion; MiR-34a was down-regulated in CRC cells and inversely correlated with FMNL2 and E2F5 expressions; Our study suggests that miR-34a is an important tumor suppressor of CRC progression by targeting FMNL2 and E2F5 thus providing new insight into the molecular mechanisms underlying CRC progression and establishing a strong potential for the application of miR-34a as a novel therapeutic marker against CRC progression colorectal cancer hsa-miR-361-5p E2F5 0.0107519600643302 0.882230614095565 0.0588682197727923 0.330185668403852 miRanda -0.322939709478388 8.03642846548624e-15 NA NA NA hsa-miR-378a-3p E2F5 0.251965146196782 0.0137483824840962 0.0588682197727923 0.330185668403852 miRNAWalker2_validate -0.107262635569783 2.24314322750755e-05 NA NA NA hsa-miR-421 E2F5 -0.171573235029879 0.0518112933651029 0.0588682197727923 0.330185668403852 miRanda -0.123407442869426 1.18649561059048e-05 NA NA NA hsa-miR-491-5p E2F5 -0.749881113074983 8.22859263179194e-09 0.0588682197727923 0.330185668403852 miRanda -0.14139730216432 1.01424139386807e-14 NA NA NA hsa-miR-495-3p E2F5 -0.421982588962504 0.00504424038526901 0.0588682197727923 0.330185668403852 MirTarget -0.149760519377058 5.64900966419551e-21 NA NA NA hsa-miR-496 E2F5 -0.139833482232877 0.39597541862532 0.0588682197727923 0.330185668403852 miRanda -0.115511681808195 9.98028339993487e-15 NA NA NA hsa-miR-543 E2F5 -0.347737549779625 0.0157394585079683 0.0588682197727923 0.330185668403852 miRanda -0.144881370667672 2.580840700923e-17 NA NA NA hsa-miR-590-3p E2F5 0.312951515438396 0.000480455423843996 0.0588682197727923 0.330185668403852 MirTarget;PITA;miRanda;miRNATAP -0.117293456245653 2.14146577898333e-05 NA NA NA hsa-miR-590-5p E2F5 0.16946027035949 0.0386004382081678 0.0588682197727923 0.330185668403852 miRanda -0.133763979755561 1.21103080876438e-05 NA NA NA hsa-miR-664a-3p E2F5 0.0413918462467198 0.611334357897022 0.0588682197727923 0.330185668403852 MirTarget -0.231398489147684 4.75334090392424e-14 NA NA NA hsa-miR-98-5p E2F5 -0.0806971683871618 0.251777941211683 0.0588682197727923 0.330185668403852 MirTarget -0.286547111947707 3.02161055445878e-13 NA NA NA hsa-miR-149-5p EP300 -0.0535821592029952 0.657713892012548 -0.0809024456519438 0.228129652996621 miRNAWalker2_validate -0.118369420853507 3.40308138671425e-09 NA NA NA hsa-miR-186-5p EP300 0.0606381894130505 0.514217717592464 -0.0809024456519438 0.228129652996621 miRNATAP -0.120697160730751 2.08389735806641e-05 NA NA NA hsa-miR-200b-3p EP300 0.245768526504511 0.0580744465744737 -0.0809024456519438 0.228129652996621 miRNAWalker2_validate;TargetScan -0.113063865997244 1.22732110177343e-09 NA NA NA hsa-miR-200c-3p EP300 0.413752622631695 8.6936333715621e-06 -0.0809024456519438 0.228129652996621 miRNAWalker2_validate;miRNATAP -0.154403328800266 1.16945030241261e-09 NA NA NA hsa-miR-22-3p EP300 0.0277287593466351 0.809325106832648 -0.0809024456519438 0.228129652996621 MirTarget;miRNATAP -0.129040932214718 7.43865109883095e-06 NA NA NA hsa-miR-2355-3p EP300 0.186207523505296 0.0525918026993012 -0.0809024456519438 0.228129652996621 MirTarget;miRNATAP -0.10892387366383 1.09126064579299e-05 NA NA NA hsa-miR-26b-5p EP300 -0.0984536538762768 0.231803769574623 -0.0809024456519438 0.228129652996621 miRNAWalker2_validate;miRNATAP -0.146408928599053 2.57076047853747e-05 NA NA NA hsa-miR-29b-1-5p EP300 0.191335428755774 0.00846436454790702 -0.0809024456519438 0.228129652996621 MirTarget -0.110260412848281 0.000822430640380143 NA NA NA hsa-miR-30c-5p EP300 -0.0469114469943097 0.565934557464627 -0.0809024456519438 0.228129652996621 miRNAWalker2_validate -0.312468973887759 2.06637782937568e-17 NA NA NA hsa-miR-339-5p EP300 0.273253751377152 0.0185300170790638 -0.0809024456519438 0.228129652996621 miRanda -0.139872022902314 5.63823302029633e-12 NA NA NA hsa-miR-342-3p EP300 -0.125683829770877 0.105810751640137 -0.0809024456519438 0.228129652996621 MirTarget;PITA;miRanda;miRNATAP -0.186971239869884 5.16619501373405e-08 NA NA NA hsa-miR-361-3p EP300 -0.0798653643614555 0.271064714084553 -0.0809024456519438 0.228129652996621 PITA -0.179432283394391 1.94960435203698e-06 NA NA NA hsa-miR-574-3p EP300 0.254057161073935 0.0219522676746007 -0.0809024456519438 0.228129652996621 PITA -0.118613580916583 3.76846499771262e-08 NA NA NA hsa-miR-590-3p EP300 0.312951515438396 0.000480455423843996 -0.0809024456519438 0.228129652996621 MirTarget;PITA;miRanda;mirMAP;miRNATAP -0.112448802446131 2.42909707997915e-05 NA NA NA hsa-let-7c-5p ESPL1 -0.04295851886768 0.676052842748691 1.60576935035459 1.37249965458092e-20 MirTarget -0.328142941017493 0.00191216593575218 NA NA NA hsa-miR-125a-5p ESPL1 -0.0833349424517387 0.325440200253607 1.60576935035459 1.37249965458092e-20 miRanda -0.550995683831132 1.29776403166629e-06 NA NA NA hsa-miR-129-5p GADD45A -0.567133990117126 0.00116152215679936 0.578892696032557 1.73109905217762e-08 miRanda -0.117655587237976 4.85565235622386e-06 NA NA NA hsa-miR-504-5p GADD45A -0.510726779625117 6.12122269907368e-05 0.578892696032557 1.73109905217762e-08 miRNAWalker2_validate -0.168708037304693 1.35009592733874e-06 NA NA NA hsa-miR-767-3p GADD45B -0.429854368420513 0.000821445291167688 0.336709257563835 0.00421134915835084 miRNATAP -0.202993831911469 2.25161118087796e-07 NA NA NA hsa-miR-107 GADD45G -0.121070851008387 0.198381281894242 -0.285061728022188 0.0219160171580943 miRanda -0.395129731583354 1.47199359369081e-11 NA NA NA hsa-miR-127-3p GADD45G -0.164565333886312 0.2710118155902 -0.285061728022188 0.0219160171580943 miRanda;miRNATAP -0.22122061367908 1.46503376303966e-09 NA NA NA hsa-miR-1468-5p GADD45G -0.00455857880561572 0.960319769044627 -0.285061728022188 0.0219160171580943 MirTarget -0.287534063688522 5.82428979745539e-07 NA NA NA hsa-miR-1976 GADD45G 0.127074435034705 0.277235601775457 -0.285061728022188 0.0219160171580943 MirTarget -0.288291002566395 7.11683611697151e-11 NA NA NA hsa-miR-135a-5p GSK3B -0.122734612943241 0.196985964641886 -0.00812669327682514 0.891858643750953 miRNATAP -0.155009045241408 4.27981971726539e-12 NA NA NA hsa-miR-23a-3p GSK3B 0.334693010651106 0.00144448147063433 -0.00812669327682514 0.891858643750953 mirMAP;miRNATAP -0.109796590797861 1.08992592703854e-06 NA NA NA hsa-miR-26a-5p GSK3B 0.033823776747294 0.726610414342369 -0.00812669327682514 0.891858643750953 miRNAWalker2_validate;miRNATAP -0.124162553965791 1.9677050059957e-06 NA NA NA hsa-miR-26b-5p GSK3B -0.0984536538762768 0.231803769574623 -0.00812669327682514 0.891858643750953 miRNATAP -0.242462216035707 2.93659997949065e-16 NA NA NA hsa-miR-27a-3p GSK3B 0.303760032895792 0.00457375391189806 -0.00812669327682514 0.891858643750953 miRNATAP -0.117289047610676 2.03194827601435e-08 NA NA NA hsa-miR-28-5p GSK3B 0.104003572105781 0.153594705579534 -0.00812669327682514 0.891858643750953 miRanda;miRNATAP -0.153581288033644 5.93445649657903e-07 NA NA NA hsa-miR-29b-3p GSK3B -0.178522712044497 0.0856106432165465 -0.00812669327682514 0.891858643750953 miRTarBase;miRNATAP -0.103024519445492 4.4831296698937e-06 NA NA NA hsa-miR-30a-3p GSK3B 0.0655410309550977 0.509541256241954 -0.00812669327682514 0.891858643750953 miRNATAP -0.169749215445873 3.72672045563906e-10 NA NA NA hsa-miR-320a GSK3B 0.092135599636725 0.243028758989929 -0.00812669327682514 0.891858643750953 miRanda;mirMAP -0.157366520986088 1.86207365140069e-06 NA NA NA hsa-miR-320c GSK3B 0.343259124189966 0.000248338401291051 -0.00812669327682514 0.891858643750953 miRanda;mirMAP -0.105871202268296 3.45649295394405e-06 NA NA NA hsa-miR-362-5p GSK3B -0.0730185557229279 0.425572997292224 -0.00812669327682514 0.891858643750953 miRNATAP -0.14675268746991 1.54588705430119e-10 NA NA NA hsa-miR-374b-5p GSK3B -0.210113949579608 0.0056866083818614 -0.00812669327682514 0.891858643750953 mirMAP -0.110240926558385 0.000413708905199806 NA NA NA hsa-miR-590-3p GSK3B 0.312951515438396 0.000480455423843996 -0.00812669327682514 0.891858643750953 miRanda;miRNATAP -0.123129209082978 1.01101816997511e-07 NA NA NA hsa-miR-664a-3p GSK3B 0.0413918462467198 0.611334357897022 -0.00812669327682514 0.891858643750953 mirMAP -0.156185915215911 2.17302109400583e-09 NA NA NA hsa-miR-708-5p GSK3B -0.00936519718301376 0.919857671524527 -0.00812669327682514 0.891858643750953 miRNATAP -0.121813780965153 5.84337104157163e-07 NA NA NA hsa-miR-99a-3p GSK3B -0.122217114840767 0.0886582098510828 -0.00812669327682514 0.891858643750953 miRNATAP -0.17367661337822 1.70824739875318e-08 NA NA NA hsa-miR-125b-2-3p HDAC2 -0.0775572585326127 0.381771874712035 0.117454388089981 0.0275001149595677 mirMAP -0.135068729475954 8.74704792504135e-10 NA NA NA hsa-miR-200b-3p HDAC2 0.245768526504511 0.0580744465744737 0.117454388089981 0.0275001149595677 mirMAP -0.108134796730082 7.60970721804197e-15 NA NA NA hsa-miR-22-5p HDAC2 0.0437233965539527 0.639996108995995 0.117454388089981 0.0275001149595677 mirMAP -0.135686893658284 2.23479196555191e-12 NA NA NA hsa-miR-30a-3p HDAC2 0.0655410309550977 0.509541256241954 0.117454388089981 0.0275001149595677 mirMAP -0.238056758666704 1.07148980415189e-25 NA NA NA hsa-miR-30a-5p HDAC2 -0.062942936611238 0.513851954700252 0.117454388089981 0.0275001149595677 mirMAP -0.380194072408458 1.03603493573615e-39 NA NA NA hsa-miR-30b-5p HDAC2 -0.0858101016322106 0.263243418521854 0.117454388089981 0.0275001149595677 mirMAP -0.107304218134878 0.000164298888604299 NA NA NA hsa-miR-30c-5p HDAC2 -0.0469114469943097 0.565934557464627 0.117454388089981 0.0275001149595677 mirMAP -0.175389058087692 5.44225528673309e-10 NA NA NA hsa-miR-30d-3p HDAC2 0.0854049347792898 0.20691254801121 0.117454388089981 0.0275001149595677 mirMAP -0.121864269874971 6.02521665649186e-06 NA NA NA hsa-miR-30d-5p HDAC2 -0.0607010868720046 0.471410043548794 0.117454388089981 0.0275001149595677 mirMAP -0.224918302149881 2.29145076227404e-12 NA NA NA hsa-miR-30e-3p HDAC2 0.00173769765874887 0.984965501547409 0.117454388089981 0.0275001149595677 mirMAP -0.178495670225385 1.53763140782324e-11 NA NA NA hsa-miR-30e-5p HDAC2 0.0764521828640934 0.41904991629622 0.117454388089981 0.0275001149595677 mirMAP -0.156994014679323 4.22533409188184e-09 NA NA NA hsa-miR-361-5p HDAC2 0.0107519600643302 0.882230614095565 0.117454388089981 0.0275001149595677 mirMAP -0.102733579511706 0.000935763380066312 NA NA NA hsa-miR-92b-3p HDAC2 -0.213968504161963 0.0523921855715716 0.117454388089981 0.0275001149595677 mirMAP -0.108197780966655 1.11181974623557e-08 NA NA NA hsa-miR-139-5p MAD2L1 -0.647754756897633 2.15378307237163e-06 0.509480359167551 2.43743048341279e-11 miRanda -0.121316505097684 1.05404947489321e-06 NA NA NA hsa-miR-124-3p MAD2L2 -0.506026514549291 0.0078056370358648 0.286320755341181 4.19217113366246e-05 miRNAWalker2_validate -0.104981581147805 1.57915656211046e-13 NA NA NA hsa-miR-1296-5p MCM2 -0.54372484392435 2.8372994306455e-07 0.719968273129628 2.13103840807418e-17 miRNAWalker2_validate;miRTarBase -0.13981438316618 7.55972739017763e-05 20332239 Here we examined the role of MCM2 in prostate cancer and the effect of microRNA-1296 miR-1296 genistein and trichostatin A TSA on the MCM complex prostate cancer hsa-miR-139-5p MCM2 -0.647754756897633 2.15378307237163e-06 0.719968273129628 2.13103840807418e-17 miRanda -0.199905099988402 2.15355073551115e-13 NA NA NA hsa-miR-491-5p MCM2 -0.749881113074983 8.22859263179194e-09 0.719968273129628 2.13103840807418e-17 miRanda -0.185614275535005 1.1284484619005e-11 NA NA NA hsa-miR-107 MCM5 -0.121070851008387 0.198381281894242 0.432885876193879 2.31259241187603e-12 miRNATAP -0.124019881371822 1.28846815999227e-05 NA NA NA hsa-miR-184 MCM5 -0.842534984406592 6.08844932016991e-08 0.432885876193879 2.31259241187603e-12 miRanda -0.106450589673324 2.15508970703347e-11 NA NA NA hsa-miR-490-3p MCM5 -0.834268051506884 5.26203010743215e-06 0.432885876193879 2.31259241187603e-12 miRanda -0.120441448842492 3.86416434500974e-19 NA NA NA hsa-miR-885-5p MCM5 -0.423614695846868 1.14213289528402e-05 0.432885876193879 2.31259241187603e-12 miRNAWalker2_validate;miRTarBase -0.212344347135582 4.29670933033879e-16 NA NA NA hsa-miR-1180-3p MCM6 -0.241468809940139 0.0100726193195657 0.382210121834667 5.7170168183565e-11 miRNAWalker2_validate -0.142784776832638 2.29167692232489e-08 NA NA NA hsa-miR-184 MCM6 -0.842534984406592 6.08844932016991e-08 0.382210121834667 5.7170168183565e-11 miRanda -0.110953804438773 2.99706866099477e-14 NA NA NA hsa-miR-30c-1-3p MCM6 0.0175008295781193 0.849381871022214 0.382210121834667 5.7170168183565e-11 MirTarget -0.118435099735543 4.16444911589167e-06 NA NA NA hsa-miR-30c-2-3p MCM6 -0.18630560418806 0.0313113706136533 0.382210121834667 5.7170168183565e-11 MirTarget -0.199052315341583 6.71552331613435e-12 NA NA NA hsa-miR-491-5p MCM6 -0.749881113074983 8.22859263179194e-09 0.382210121834667 5.7170168183565e-11 miRanda -0.121059680336253 2.65266191177361e-12 NA NA NA hsa-miR-589-3p MCM6 0.00739371736361305 0.927768051649329 0.382210121834667 5.7170168183565e-11 MirTarget -0.10833753329702 0.000184510222213164 NA NA NA hsa-miR-7-1-3p MCM6 -0.0894475316162393 0.37322359785108 0.382210121834667 5.7170168183565e-11 mirMAP -0.105910247794449 5.90920091687466e-06 NA NA NA hsa-miR-107 MCM7 -0.121070851008387 0.198381281894242 0.304071192262941 2.6811439400517e-05 miRanda -0.199555025278988 8.62299180616376e-11 NA NA NA hsa-miR-193b-3p MCM7 0.0766333116224072 0.324723824453143 0.304071192262941 2.6811439400517e-05 miRNAWalker2_validate -0.11584232045451 0.00203242694148569 NA NA NA hsa-miR-222-3p MCM7 0.107117065575197 0.423818119725697 0.304071192262941 2.6811439400517e-05 miRNAWalker2_validate -0.153701545790602 2.37162666816492e-14 NA NA NA hsa-miR-34a-5p MCM7 0.143472919835524 0.211283228567057 0.304071192262941 2.6811439400517e-05 miRNAWalker2_validate -0.201268756136123 6.19699802686052e-17 NA NA NA hsa-miR-484 MCM7 -0.0190828819137732 0.802298900989652 0.304071192262941 2.6811439400517e-05 miRNAWalker2_validate -0.184154635598248 2.12714083905869e-06 NA NA NA hsa-miR-3065-5p MDM2 -0.262608140481507 0.0414570974148034 0.179468554373188 0.00090619936510014 mirMAP -0.108004752778931 9.0880493197318e-14 NA NA NA hsa-miR-374b-5p MDM2 -0.210113949579608 0.0056866083818614 0.179468554373188 0.00090619936510014 mirMAP -0.115805376151209 4.29838757263469e-05 NA NA NA hsa-miR-504-5p MDM2 -0.510726779625117 6.12122269907368e-05 0.179468554373188 0.00090619936510014 miRNAWalker2_validate -0.122605313729819 6.11646737446648e-17 NA NA NA hsa-miR-7-1-3p MDM2 -0.0894475316162393 0.37322359785108 0.179468554373188 0.00090619936510014 mirMAP -0.103468424775092 4.37531630762283e-08 NA NA NA hsa-miR-9-3p MDM2 -0.184945845984695 0.0290051908009009 0.179468554373188 0.00090619936510014 mirMAP -0.122381796737815 5.87019491428867e-05 NA NA NA hsa-let-7c-5p MYC -0.04295851886768 0.676052842748691 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.187493318710893 0.00114816000552179 22128178 MicroRNA let 7c suppresses androgen receptor expression and activity via regulation of Myc expression in prostate cancer cells prostate cancer hsa-let-7f-5p MYC 0.0508269185845673 0.62677515045793 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.234906036376788 1.38501616912732e-05 NA NA NA hsa-let-7g-5p MYC -0.0253396334608382 0.742556489443668 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.402035322238661 2.54889860814805e-08 NA NA NA hsa-miR-145-5p MYC 0.108948399943015 0.310032352776693 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.26620175812587 1.77011092539307e-10 23919393;21836381;23499891;24356567;21092188;21690566;21723890;27482839 MicroRNA 145 function as a cell growth repressor by directly targeting c Myc in human ovarian cancer; We show that miR-145 directly target the c-Myc 3'-UTR; Moreover ectopic expression of c-Myc reduces the inhibition of cell proliferation caused by miR-145 transfection; Cell cycle assay showed that up-regulation of miR-145 reduces S phase population and restoration of c-Myc can rescue this reduction; These findings indicate that miR-145 inhibits cell proliferation and promotes cell apoptosis by targeting c-Myc 3'-UTR;The ectopic expression of miR-145 showed a significant growth inhibition in both canine and human melanoma cells tested and the effect was achieved partly by suppressing c-MYC in canine melanoma LMeC and in human melanoma A2058 and Mewo cells;The ectopic expression of miR-145 inhibited the growth of DLD-1 cells by disturbing β-catenin translocation into the nucleus thereby leading to the down-regulation of LEF/TCF transcriptional target genes c-Myc and CyclinD1;miR 145 inhibits proliferation and invasion of esophageal squamous cell carcinoma in part by targeting c Myc; The impact of overexpression of miR-145 on putative target c-Myc was subsequently confirmed via Western blot; Overexpression of miR-145 significantly decreased p < 0.05 the protein level of c-Myc which has previously been identified as a direct target of miR-145; Our results demonstrate that overexpression of miR-145 inhibits tumor growth in part by targeting c-Myc;In colon cancer cells c-Myc is a confirmed direct target for miR-145; The aim of this work was to investigate the effect of miR-145 and c-Myc on proliferation of NSCLC cells using the NSCLC cell lines A549 and H23 as models; We further showed that c-Myc was a direct target for miR-145; Taken together our study results demonstrate that miR-145 inhibits proliferation of NSCLC cells through c-Myc;miR-145 delivery reduced tumor proliferation and increased apoptosis with concomitant repression of c-Myc and ERK5 as novel regulatory target of miR-145;We found a reverse-correlation between the expression of miR-145 and its target genes such as fascin-1 c-myc SMAD2/3 and IGF-1R in breast cancer cell lines and breast cancer patient tissues;Consequently the increased miR-145 induced G1 cell cycle arrest reduced tumor proliferation and increased apoptosis inhibited HCT-116 cell migration and suppressed c-MYC expressions a regulatory target of miR-145 ;;;;;;;cell migration ovarian cancer;melanoma;colon cancer;esophageal cancer;lung squamous cell cancer;colon cancer;breast cancer;colon cancer hsa-miR-24-3p MYC 0.133257261462871 0.212960921400853 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.297311537056991 1.14667789168757e-11 NA NA NA hsa-miR-26a-5p MYC 0.033823776747294 0.726610414342369 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.349840372726607 4.11115213986892e-11 21901171 Our results identify new molecular targets of c-Myc in AML and highlight miR-26a attractiveness as a therapeutic target in leukemia acute myeloid leukemia hsa-miR-335-5p MYC 0.0938511632756978 0.336889056387694 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.221233805698315 6.63171764745164e-07 NA NA NA hsa-miR-34a-5p MYC 0.143472919835524 0.211283228567057 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.469833736893329 6.08752675410229e-40 25572695;25686834;21460242;22159222;23640973;22830357;22235332 The c-Myc and CD44 were confirmed as direct targets of miR-34a in EJ cell apoptosis induced by PRE;miR 34a induces cellular senescence via modulation of telomerase activity in human hepatocellular carcinoma by targeting FoxM1/c Myc pathway;Myc mediated repression of microRNA 34a promotes high grade transformation of B cell lymphoma by dysregulation of FoxP1;MicroRNA 34a suppresses malignant transformation by targeting c Myc transcriptional complexes in human renal cell carcinoma; We investigated the functional effects of microRNA-34a miR-34a on c-Myc transcriptional complexes in renal cell carcinoma; miR-34a down-regulated expression of multiple oncogenes including c-Myc by targeting its 3' untranslated region which was revealed by luciferase reporter assays; Our results demonstrate that miR-34a suppresses assembly and function of the c-Myc complex that activates or elongates transcription indicating a novel role of miR-34a in the regulation of transcription by c-Myc;Among them miR-34a was also associated with poor prognosis in 2 independent series of leukemic and nodal MCL and in cooperation with high expression of the MYC oncogene;We report that miR-34a did not inhibit cell proliferation notwithstanding a marked down-regulation of c-MYC;MicroRNA 34a modulates c Myc transcriptional complexes to suppress malignancy in human prostate cancer cells; We studied the functional effects of miR-34a on c-Myc transcriptional complexes in PC-3 prostate cancer cells; miR-34a downregulated expression of c-Myc oncogene by targeting its 3' UTR as shown by luciferase reporter assays; This is the first report to document that miR-34a suppresses assembly and function of the c-Myc-Skp2-Miz1 complex that activates RhoA and the c-Myc-pTEFB complex that elongates transcription of various genes suggesting a novel role of miR-34a in the regulation of transcription by c-Myc complex ;;;malignant trasformation;worse prognosis;; bladder cancer;liver cancer;B cell lymphoma;kidney renal cell cancer;lymphoma;lymphoma;prostate cancer hsa-miR-34c-5p MYC 0.181931113688093 0.257266965617363 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase;miRanda -0.212118906251778 1.73484497587865e-16 NA NA NA hsa-miR-377-5p MYC -0.254102489855821 0.081507589808783 0.0342990533418108 0.742963156666191 miRNATAP -0.197215758202484 1.41992056070433e-11 NA NA NA hsa-miR-378a-3p MYC 0.251965146196782 0.0137483824840962 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.135286330402019 0.00204077080845563 NA NA NA hsa-miR-590-3p MYC 0.312951515438396 0.000480455423843996 0.0342990533418108 0.742963156666191 miRanda -0.223628987104378 2.58191493375387e-06 24200043 We identified important regulators MYC NFKB1 miR-590 and miR-570 and significant miRNA-TF synergistic regulatory motifs by random simulations lung squamous cell cancer hsa-miR-744-5p MYC -0.31868579180387 0.000969037378618306 0.0342990533418108 0.742963156666191 miRNAWalker2_validate -0.208939879123018 7.28554627025748e-06 24991193 Decrease expression of microRNA 744 promotes cell proliferation by targeting c Myc in human hepatocellular carcinoma; Quantitative reverse-transcription polymerase chain reaction qRT-PCR was conducted to detect the expression of miR-744 and Immunohistochemistry was performed to detect expression of c-Myc in HCC specimens and adjacent normal tissues; Luciferase reporter assays was performed to confirm whether miR-744 regulated the expression of c-Myc; Luciferase assay and Western blot analysis revealed that c-Myc is a direct target of miR-744; Down-regulation of miR-744 and up-regulation of c-Myc were detected in HCC specimens compared with adjacent normal tissues; Moreover restoration of miR-744 rescues c-Myc induced HCC proliferation; Our data suggest that miR-744 exerts its tumor suppressor function by targeting c-Myc leading to the inhibition of HCC cell growth liver cancer hsa-miR-98-5p MYC -0.0806971683871618 0.251777941211683 0.0342990533418108 0.742963156666191 miRNAWalker2_validate;miRTarBase -0.482234879670646 1.25183084383233e-12 NA NA NA hsa-miR-338-5p PCNA -0.424003687569629 0.00246415720301323 0.463982922014727 2.18911772155955e-14 PITA -0.105432694775061 5.63669972278373e-10 NA NA NA hsa-miR-490-3p PCNA -0.834268051506884 5.26203010743215e-06 0.463982922014727 2.18911772155955e-14 miRanda -0.109348728305673 9.91122859029426e-18 NA NA NA hsa-miR-100-5p PLK1 0.0939087252225708 0.437623927320764 0.87224444496368 4.66430223830671e-18 miRNAWalker2_validate;miRTarBase -0.152000910857058 0.000872463848776063 23151088;22246341;23842624;25537513;22120675;21636267 MicroRNA 100 is a potential molecular marker of non small cell lung cancer and functions as a tumor suppressor by targeting polo like kinase 1; By using microRNA miR target prediction algorithms we identified miR-100 that might potentially bind the 3'-untranslated region of PLK1 transcripts; The purpose of this study was to investigate the roles of miR-100 and its association with PLK1 in NSCLC development; Finally the effects of miR-100 expression on growth apoptosis and cell cycle of NSCLC cells by posttranscriptionally regulating PLK1 expression were determined; Meanwhile miR-100 mimics could significantly inhibit PLK1 mRNA and protein expression and reduce the luciferase activity of a PLK1 3' untranslated region-based reporter construct in A549 cells; Furthermore small interfering RNA siRNA-mediated PLK1 downregulation could mimic the effects of miR-100 mimics while PLK1 overexpression could partially rescue the phenotypical changes of NSCLC cells induced by miR-100 mimics; Our findings indicate that low miR-100 may be a poor prognostic factor for NSCLC patients and functions as a tumor suppressor by posttranscriptionally regulating PLK1 expression;Together these results suggest that low miR-100 expression may be an independent poor prognostic factor and miR-100 can function as a tumor suppressor by targeting PLK1 in human EOCs;In HCC tissues miR-100 expression was inversely correlated with the expression of plk1 protein r = -0.418; P = 0.029; Therefore downregulation of miR-100 was correlated with progressive pathological feature and poor prognosis in HCC patients and miR-100 could function as a tumor suppressor by targeting plk1;Here we show that miR-100 inhibits maintenance and expansion of BrCSCs in basal-like cancer through Polo-like kinase1 Plk1 down-regulation;MiR 100 resensitizes docetaxel resistant human lung adenocarcinoma cells SPC A1 to docetaxel by targeting Plk1; Knock-down of Plk1 which was a direct target of miR-100 yielded similar effects as that of ectopic miR-100 expression; The inverse correlation between miR-100 and Plk1 expression was also detected in nude mice SPC-A1/DTX tumor xenografts and clinical lung adenocarcinoma tissues and was proved to be related with the in vivo response to docetaxel; Thus our results suggested that down-regulation of miR-100 could lead to Plk1 over-expression and eventually to docetaxel chemoresistance of human lung adenocarcinoma;Reduced miR 100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein; Through modulating miR-100 expression using miR-100 inhibitor or mimic in vitro cell growth cycle and apoptosis were tested separately by MTT or flow cytometry and meanwhile Polo-like kinase1 PLK1 mRNA and protein expressions were detected by qRT-PCR and immunoblotting; The expression of PLK1 in 125 cervical tissues was also examined by immunohistochemical staining and the correlation between miR-100 and PLK1 expression in the same tissues was analysed; The modulation of miR-100 expression remarkably influenced cell proliferation cycle and apoptosis as well as the level of PLK1 protein but not mRNA in vitro experiments; PLK1 expression was negatively correlated with miR-100 expression in CIN3 and cervical cancer tissues; The reduced miR-100 expression participates in the development of cervical cancer at least partly through loss of inhibition to target gene PLK1 which probably occurs in a relative late phase of carcinogenesis ;;progression;worse prognosis;;drug resistance;tumorigenesis lung squamous cell cancer;ovarian cancer;liver cancer;breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-874-3p PLK1 -0.342846516198707 0.00227406462925841 0.87224444496368 4.66430223830671e-18 miRNAWalker2_validate -0.370915577018944 1.21771804839937e-20 NA NA NA hsa-miR-335-3p RAD21 0.34540584335595 0.00043848660222294 -0.0700031391745988 0.205655811684518 mirMAP -0.100592075603885 1.245269517582e-10 NA NA NA hsa-miR-361-5p RAD21 0.0107519600643302 0.882230614095565 -0.0700031391745988 0.205655811684518 PITA;miRanda;miRNATAP -0.142388782162939 2.45040871684586e-08 NA NA NA hsa-miR-139-5p RB1 -0.647754756897633 2.15378307237163e-06 0.102426353692786 0.0803433627131719 miRanda -0.118561872957923 2.0386521698372e-14 NA NA NA hsa-miR-495-3p RBL1 -0.421982588962504 0.00504424038526901 0.435091009019365 1.54394939191296e-07 mirMAP -0.111598382200941 3.34030029490157e-06 NA NA NA hsa-miR-7-1-3p RBL1 -0.0894475316162393 0.37322359785108 0.435091009019365 1.54394939191296e-07 mirMAP -0.187135442928637 2.20568294002692e-07 NA NA NA hsa-miR-130b-3p SKP1 0.503911467685709 9.46590588950823e-08 -0.190804442552597 0.000494555589918693 MirTarget -0.122601330293596 1.00997929262659e-19 NA NA NA hsa-miR-138-5p SKP2 -0.616087649920851 1.35200027885729e-05 0.304483103343004 2.39516717741366e-06 MirTarget -0.112767222560839 6.70974775053186e-09 NA NA NA hsa-miR-30b-5p SKP2 -0.0858101016322106 0.263243418521854 0.304483103343004 2.39516717741366e-06 MirTarget;miRNATAP -0.221841956637276 3.20590527286436e-07 NA NA NA hsa-miR-15a-5p SMAD3 0.142658985373611 0.0536219185009004 -0.0461774445110805 0.498736701509296 MirTarget -0.137331699251882 0.000744677232811907 NA NA NA hsa-let-7f-1-3p SMAD4 0.110713086694053 0.21484524300518 -0.0480651463186925 0.333853878951855 mirMAP -0.112094816748955 3.72889244611741e-12 NA NA NA hsa-miR-106a-5p SMAD4 0.110074064773897 0.13045815990621 -0.0480651463186925 0.333853878951855 mirMAP;miRNATAP -0.104621077552926 8.44868166811478e-07 NA NA NA hsa-miR-125a-5p SMAD4 -0.0833349424517387 0.325440200253607 -0.0480651463186925 0.333853878951855 miRTarBase -0.108065804207878 3.53979250363128e-07 NA NA NA hsa-miR-126-5p SMAD4 0.177332403948664 0.0711675116243123 -0.0480651463186925 0.333853878951855 mirMAP -0.108936745818454 1.3197797103689e-12 NA NA NA hsa-miR-146a-5p SMAD4 0.148942451196282 0.114229072828724 -0.0480651463186925 0.333853878951855 miRNAWalker2_validate;miRTarBase -0.12304740941773 1.20646297394653e-15 21982769;27438138;22020746 The results indicated that miR-146a regulated the sensitivity of HCC cells to the cytotoxic effects of IFN-α through SMAD4 suggesting that this miRNA could be suitable for prediction of the clinical response and potential therapeutic target in HCC patients on IFN-based therapy;SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells whereas SMAD4 expression was restored by anti-miR-146a; Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4 and this effect was reversed by miR-146a overexpression; In stomach cancer tissues miR-146a was expressed at higher levels and more frequent NFκB nuclear positivity immunohistochemically but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group; In conclusion EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4 thereby contributing to EBV-induced stomach cancer progression;Increased miR 146a in gastric cancer directly targets SMAD4 and is involved in modulating cell proliferation and apoptosis; Using target prediction algorithms luciferase reporter assay and Western blot assay SMAD family member 4 SMAD4 was identified as a target gene of miR-146a in gastric cancer; Moreover an inverse correlation was observed between the expression of SMAD4 mRNA and miR-146a in gastric cancer tissues R=-0.731 P=0.039 Pearson's correlation; Taken together our results provide important evidence that miR-146a can directly target SMAD4 and suggest that miR-146a may play a role in the development of gastric cancer by modulating cell proliferation and apoptosis drug resistance;progression; liver cancer;gastric cancer;gastric cancer hsa-miR-146b-5p SMAD4 0.441078059667297 0.000237619929889385 -0.0480651463186925 0.333853878951855 PITA;miRanda;miRNATAP -0.147840875731319 5.77711341775598e-35 21874046;26883911 MicroRNA miR 146b 5p regulates signal transduction of TGF β by repressing SMAD4 in thyroid cancer; A computational search revealed that miR-146b-5p putatively binds to the 3' untranslated region UTR of SMAD4 an important member of the transforming growth factor β TGF-β signaling pathway; Luciferase reporter assay confirmed the direct binding of miR-146b-5p on the SMAD4 3'UTR; Specific inhibition of miR-146b-5p with a locked nucleic acid-modified anti-miR-146b oligonucleotide significantly increased SMAD4 levels in the human papillary carcinoma cell lines TPC-1 and BCPAP; The overexpression of miR-146b-5p in normal rat follicular PCCL3 cells decreased SMAD4 levels and disrupted TGF-β signal transduction;The overexpression of SMAD4 in BCPAP cells a validated target of miR-146b-5p and key protein in the TGF-β signaling pathway inhibited migration similarly to the effects observed with the antagomiR 146b-5p ; thyroid cancer;thyroid cancer hsa-miR-150-5p SMAD4 0.0645271721519611 0.513295658828718 -0.0480651463186925 0.333853878951855 mirMAP -0.140099817794943 5.26740000263147e-21 NA NA NA hsa-miR-155-5p SMAD4 0.969837380981085 5.86551495710056e-15 -0.0480651463186925 0.333853878951855 miRNAWalker2_validate -0.118153900154786 3.91770964859782e-27 27626488 Here we demonstrated that TGF-β1 elevated the expression of miR-155 in colorectal cancer cells through SMAD3 and SMAD4 colorectal cancer hsa-miR-199a-5p SMAD4 0.174889610847968 0.0900027418912454 -0.0480651463186925 0.333853878951855 miRNAWalker2_validate;miRTarBase;miRanda -0.100954911386107 9.72901116309968e-13 NA NA NA hsa-miR-2355-5p SMAD4 0.00808596988211185 0.916165501155096 -0.0480651463186925 0.333853878951855 MirTarget -0.138601553013193 2.31055516575799e-12 NA NA NA hsa-miR-27b-3p SMAD4 -0.0599187745243146 0.58208143475992 -0.0480651463186925 0.333853878951855 MirTarget -0.109946664636984 4.80354118087017e-14 NA NA NA hsa-miR-28-3p SMAD4 0.139444423971018 0.0811187936164314 -0.0480651463186925 0.333853878951855 mirMAP -0.156323645997406 2.52937793682202e-11 NA NA NA hsa-miR-28-5p SMAD4 0.104003572105781 0.153594705579534 -0.0480651463186925 0.333853878951855 miRanda;miRNATAP -0.108665505252487 4.7241141004314e-07 NA NA NA hsa-miR-29b-2-5p SMAD4 -0.26834261955727 0.00453760540520233 -0.0480651463186925 0.333853878951855 MirTarget -0.124752999421755 6.21375734890817e-16 NA NA NA hsa-miR-30a-5p SMAD4 -0.062942936611238 0.513851954700252 -0.0480651463186925 0.333853878951855 mirMAP -0.197157226879658 9.57330245001194e-16 NA NA NA hsa-miR-30c-5p SMAD4 -0.0469114469943097 0.565934557464627 -0.0480651463186925 0.333853878951855 mirMAP -0.106012380191749 4.33019792404505e-06 NA NA NA hsa-miR-30d-5p SMAD4 -0.0607010868720046 0.471410043548794 -0.0480651463186925 0.333853878951855 mirMAP -0.103563321543632 8.62191088751512e-05 NA NA NA hsa-miR-335-3p SMAD4 0.34540584335595 0.00043848660222294 -0.0480651463186925 0.333853878951855 mirMAP -0.114385410785847 4.0809889989156e-14 NA NA NA hsa-miR-34a-5p SMAD4 0.143472919835524 0.211283228567057 -0.0480651463186925 0.333853878951855 miRNATAP -0.161359767712972 2.62012161311457e-40 22750848 Mechanistically in addition to its direct regulation of platelet-derived growth factor receptor-alpha PDGFRA promoter enrichment analysis of context likelihood of relatedness-inferred mRNA nodes established miR-34a as a novel regulator of a SMAD4 transcriptional network; Specifically in glioblastoma within the context of the global network promoter enrichment analysis of network edges uncovered a novel regulation of TGF-β signaling via a Smad4 transcriptomic network by miR-34a glioblastoma hsa-miR-361-5p SMAD4 0.0107519600643302 0.882230614095565 -0.0480651463186925 0.333853878951855 mirMAP -0.199229708620127 4.10990421373243e-16 NA NA NA hsa-miR-425-5p SMAD4 -0.0412837711174561 0.682284485592858 -0.0480651463186925 0.333853878951855 mirMAP -0.109670741700584 4.36243779451545e-13 NA NA NA hsa-miR-589-5p SMAD4 -0.0382528919634639 0.5844074434222 -0.0480651463186925 0.333853878951855 miRNATAP -0.113352850721085 3.13434103908252e-06 NA NA NA hsa-miR-664a-3p SMAD4 0.0413918462467198 0.611334357897022 -0.0480651463186925 0.333853878951855 MirTarget -0.176780902353316 2.67661742767344e-23 NA NA NA hsa-miR-664a-5p SMAD4 0.0825994937558296 0.264421724444089 -0.0480651463186925 0.333853878951855 mirMAP -0.144950554671858 1.05670261458917e-13 NA NA NA hsa-miR-7-1-3p SMAD4 -0.0894475316162393 0.37322359785108 -0.0480651463186925 0.333853878951855 mirMAP -0.107525661059734 8.73423192056203e-14 NA NA NA hsa-let-7d-5p SMC1A 0.111345229817909 0.0872634398336815 0.0731134824417063 0.223564200714282 MirTarget -0.122548043057745 0.00196664008364745 NA NA NA hsa-let-7g-5p SMC1A -0.0253396334608382 0.742556489443668 0.0731134824417063 0.223564200714282 MirTarget -0.117116804789672 0.000888133919642843 NA NA NA hsa-miR-103a-2-5p SMC1A -0.0677071239234235 0.496202425310213 0.0731134824417063 0.223564200714282 mirMAP -0.10733607097288 2.31706342377635e-07 NA NA NA hsa-miR-664a-3p SMC1A 0.0413918462467198 0.611334357897022 0.0731134824417063 0.223564200714282 mirMAP -0.118611451799251 5.36811289555196e-06 NA NA NA hsa-miR-142-3p SMC3 0.558908907203977 1.37828834702416e-05 -0.0650857161813017 0.276929640465803 miRanda -0.105014659212308 2.43563395776124e-10 NA NA NA hsa-miR-150-5p SMC3 0.0645271721519611 0.513295658828718 -0.0650857161813017 0.276929640465803 MirTarget -0.157053159298135 4.8589602204668e-13 NA NA NA hsa-miR-186-5p SMC3 0.0606381894130505 0.514217717592464 -0.0650857161813017 0.276929640465803 MirTarget -0.135596665657393 4.27095226870825e-08 NA NA NA hsa-miR-193b-3p SMC3 0.0766333116224072 0.324723824453143 -0.0650857161813017 0.276929640465803 miRNAWalker2_validate -0.145914016540251 2.48209667367187e-07 NA NA NA hsa-miR-28-5p SMC3 0.104003572105781 0.153594705579534 -0.0650857161813017 0.276929640465803 miRanda -0.148153258535169 1.71641902281765e-06 NA NA NA hsa-miR-361-5p SMC3 0.0107519600643302 0.882230614095565 -0.0650857161813017 0.276929640465803 miRanda -0.27629085768772 4.09457606642501e-15 NA NA NA hsa-miR-590-3p SMC3 0.312951515438396 0.000480455423843996 -0.0650857161813017 0.276929640465803 miRanda -0.132600796109406 1.11283536689231e-08 NA NA NA hsa-miR-149-5p STAG1 -0.0535821592029952 0.657713892012548 -0.00145739505053477 0.981738006288318 miRNAWalker2_validate -0.106293536852859 4.33452629980334e-07 NA NA NA hsa-miR-199a-5p STAG1 0.174889610847968 0.0900027418912454 -0.00145739505053477 0.981738006288318 miRanda -0.106883013539997 1.33193600841466e-05 NA NA NA hsa-miR-26b-5p STAG1 -0.0984536538762768 0.231803769574623 -0.00145739505053477 0.981738006288318 miRNAWalker2_validate -0.154182422832614 2.24506947601039e-05 NA NA NA hsa-miR-30a-5p STAG1 -0.062942936611238 0.513851954700252 -0.00145739505053477 0.981738006288318 MirTarget -0.14216312597166 0.00094871311197018 NA NA NA hsa-miR-30b-5p STAG1 -0.0858101016322106 0.263243418521854 -0.00145739505053477 0.981738006288318 MirTarget -0.25888881255158 2.60478201959698e-11 NA NA NA hsa-miR-30c-5p STAG1 -0.0469114469943097 0.565934557464627 -0.00145739505053477 0.981738006288318 MirTarget -0.251613317473894 1.15508294853776e-10 NA NA NA hsa-miR-7-1-3p STAG1 -0.0894475316162393 0.37322359785108 -0.00145739505053477 0.981738006288318 MirTarget -0.11334234016848 6.00640100502778e-06 NA NA NA hsa-miR-103a-2-5p STAG2 -0.0677071239234235 0.496202425310213 -0.0218009698394264 0.695587236954735 MirTarget -0.108772787482565 2.78819785829688e-10 NA NA NA hsa-miR-140-3p STAG2 -0.123864258867705 0.14796686619449 -0.0218009698394264 0.695587236954735 MirTarget;PITA;miRNATAP -0.114001517006215 2.00859009338779e-05 NA NA NA hsa-miR-22-3p STAG2 0.0277287593466351 0.809325106832648 -0.0218009698394264 0.695587236954735 MirTarget;miRNATAP -0.152936352236525 8.08070711066481e-14 NA NA NA hsa-miR-29a-3p STAG2 -0.224140851576083 0.0349516103550236 -0.0218009698394264 0.695587236954735 MirTarget;miRNATAP -0.131416872190519 4.13434254450881e-10 NA NA NA hsa-miR-29b-3p STAG2 -0.178522712044497 0.0856106432165465 -0.0218009698394264 0.695587236954735 MirTarget;miRNATAP -0.170379383220219 3.32957900458646e-21 NA NA NA hsa-miR-29c-3p STAG2 -0.372180390387259 0.00202634431973208 -0.0218009698394264 0.695587236954735 MirTarget;miRNATAP -0.118499363671887 1.74383427748387e-14 NA NA NA hsa-miR-30a-5p STAG2 -0.062942936611238 0.513851954700252 -0.0218009698394264 0.695587236954735 MirTarget;mirMAP;miRNATAP -0.133476493199347 6.55042121490518e-06 NA NA NA hsa-miR-30b-5p STAG2 -0.0858101016322106 0.263243418521854 -0.0218009698394264 0.695587236954735 MirTarget;mirMAP -0.214182888987837 8.05410261827361e-16 NA NA NA hsa-miR-30c-5p STAG2 -0.0469114469943097 0.565934557464627 -0.0218009698394264 0.695587236954735 MirTarget;mirMAP;miRNATAP -0.180682420392771 2.03443695545984e-11 NA NA NA hsa-miR-30d-5p STAG2 -0.0607010868720046 0.471410043548794 -0.0218009698394264 0.695587236954735 MirTarget;mirMAP;miRNATAP -0.138361967598821 8.61489765900043e-06 NA NA NA hsa-miR-342-3p STAG2 -0.125683829770877 0.105810751640137 -0.0218009698394264 0.695587236954735 miRanda -0.217423762874534 2.99926905700234e-19 NA NA NA hsa-miR-34a-5p STAG2 0.143472919835524 0.211283228567057 -0.0218009698394264 0.695587236954735 miRNAWalker2_validate;miRNATAP -0.14005636313011 6.87012479985699e-21 NA NA NA hsa-miR-361-5p STAG2 0.0107519600643302 0.882230614095565 -0.0218009698394264 0.695587236954735 miRanda -0.217696372888326 6.96605367741582e-14 NA NA NA hsa-miR-495-3p STAG2 -0.421982588962504 0.00504424038526901 -0.0218009698394264 0.695587236954735 MirTarget -0.106115947099739 1.23739986803528e-21 NA NA NA hsa-miR-664a-3p STAG2 0.0413918462467198 0.611334357897022 -0.0218009698394264 0.695587236954735 mirMAP -0.13401141642957 5.54302303197345e-10 NA NA NA hsa-miR-28-5p TFDP1 0.104003572105781 0.153594705579534 -0.118722647001067 0.029904169063442 miRanda -0.123100418034977 4.55829943482585e-06 NA NA NA hsa-miR-30a-5p TFDP1 -0.062942936611238 0.513851954700252 -0.118722647001067 0.029904169063442 MirTarget;miRNATAP -0.15806642816858 3.56903619965929e-07 NA NA NA hsa-miR-30b-5p TFDP1 -0.0858101016322106 0.263243418521854 -0.118722647001067 0.029904169063442 MirTarget -0.165951627441625 4.92240208942032e-09 NA NA NA hsa-miR-30c-5p TFDP1 -0.0469114469943097 0.565934557464627 -0.118722647001067 0.029904169063442 MirTarget;miRNATAP -0.139837907118834 1.03165213533415e-06 NA NA NA hsa-miR-30d-3p TFDP1 0.0854049347792898 0.20691254801121 -0.118722647001067 0.029904169063442 MirTarget;miRNATAP -0.119570580308374 1.02091086617796e-05 NA NA NA hsa-miR-30d-5p TFDP1 -0.0607010868720046 0.471410043548794 -0.118722647001067 0.029904169063442 MirTarget;miRNATAP -0.131206844673183 6.18363521306737e-05 NA NA NA hsa-miR-30e-3p TFDP1 0.00173769765874887 0.984965501547409 -0.118722647001067 0.029904169063442 MirTarget -0.136934722526631 3.44961891992865e-07 NA NA NA hsa-miR-30e-5p TFDP1 0.0764521828640934 0.41904991629622 -0.118722647001067 0.029904169063442 MirTarget -0.128818415509482 1.87617040077901e-06 NA NA NA hsa-miR-361-5p TFDP1 0.0107519600643302 0.882230614095565 -0.118722647001067 0.029904169063442 miRanda -0.209815666196644 8.24499764709105e-12 NA NA NA hsa-miR-590-3p TFDP1 0.312951515438396 0.000480455423843996 -0.118722647001067 0.029904169063442 MirTarget;PITA;miRanda -0.116440986359596 6.93548726838812e-09 NA NA NA hsa-miR-7-1-3p TFDP1 -0.0894475316162393 0.37322359785108 -0.118722647001067 0.029904169063442 MirTarget -0.105489746467966 5.51023919253019e-09 NA NA NA hsa-miR-125a-5p TFDP2 -0.0833349424517387 0.325440200253607 0.133044187484989 0.015835219626664 mirMAP -0.149485399980342 3.99292051066665e-06 NA NA NA hsa-miR-1301-3p TFDP2 -0.105695714210758 0.152249184194132 0.133044187484989 0.015835219626664 MirTarget -0.158609705877386 4.18932544448651e-07 NA NA NA hsa-miR-22-5p TFDP2 0.0437233965539527 0.639996108995995 0.133044187484989 0.015835219626664 mirMAP -0.126299949709429 1.60053128049966e-07 NA NA NA hsa-miR-2355-5p TFDP2 0.00808596988211185 0.916165501155096 0.133044187484989 0.015835219626664 mirMAP -0.122271944493808 6.41449151443144e-05 NA NA NA hsa-miR-29b-1-5p TFDP2 0.191335428755774 0.00846436454790702 0.133044187484989 0.015835219626664 mirMAP -0.120961188351788 8.06047827024741e-05 NA NA NA hsa-miR-29b-3p TFDP2 -0.178522712044497 0.0856106432165465 0.133044187484989 0.015835219626664 mirMAP -0.158802591725687 2.25364273937557e-11 NA NA NA hsa-miR-342-3p TFDP2 -0.125683829770877 0.105810751640137 0.133044187484989 0.015835219626664 miRNATAP -0.180927916035749 1.55164983895346e-08 NA NA NA hsa-miR-34a-5p TFDP2 0.143472919835524 0.211283228567057 0.133044187484989 0.015835219626664 miRNATAP -0.165299902985788 1.6335460771484e-17 NA NA NA hsa-miR-363-3p TFDP2 -0.0500185604139096 0.527823314542991 0.133044187484989 0.015835219626664 MirTarget -0.109228682926323 0.000284730909569036 NA NA NA hsa-miR-365a-3p TFDP2 -0.184222766207919 0.0292663235724171 0.133044187484989 0.015835219626664 mirMAP -0.155936066114075 6.44978728480765e-09 NA NA NA hsa-miR-501-3p TFDP2 0.0726267165568464 0.382729320534971 0.133044187484989 0.015835219626664 MirTarget;TargetScan;miRNATAP -0.100175938799246 0.000344254920766041 NA NA NA hsa-miR-7-1-3p TFDP2 -0.0894475316162393 0.37322359785108 0.133044187484989 0.015835219626664 mirMAP -0.111348297875275 5.87235728957222e-07 NA NA NA hsa-miR-139-5p TGFB1 -0.647754756897633 2.15378307237163e-06 0.188661409906809 0.0283645098828165 miRanda -0.257914873895623 5.01185822136258e-24 NA NA NA hsa-let-7a-3p TGFB2 0.0530563276543763 0.370749834463566 0.770124480266937 2.43471584041631e-07 miRNATAP -0.442723562700544 0.000358820362673608 NA NA NA hsa-miR-181a-2-3p TGFB2 -0.0630409614874416 0.473088318215936 0.770124480266937 2.43471584041631e-07 MirTarget -0.480565043297097 4.63300164322775e-06 NA NA NA hsa-miR-9-3p TGFB2 -0.184945845984695 0.0290051908009009 0.770124480266937 2.43471584041631e-07 MirTarget -0.502297808087689 1.67671098075053e-06 NA NA NA hsa-miR-125a-5p TP53 -0.0833349424517387 0.325440200253607 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase;miRanda -0.375445863722298 1.2184854773952e-16 26389681;21777146;23079745 Mechanistically inactivation of miR-125a during cervical carcinogenesis was caused by HPV suppression of p53 expression;In addition wild-type p53 mRNA and protein expression was increased by hsa-miR-125a-5p overexpression; Moreover blocking wild-type p53 attenuated the effect of hsa-miR-125a-5p on apoptosis; In loss-of-function experiments wild-type p53 mRNA and protein expression was decreased by blocking hsa-miR-125a-5p; The effect of hsa-miR-125a-5p inhibitor on apoptosis was also weakened by blocking wild-type p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity tumorigenesis;; cervical and endocervical cancer;lung cancer;liver cancer hsa-miR-125b-5p TP53 -0.0119698382910496 0.890233923197358 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;miRTarBase -0.294628443669371 1.07324994036762e-07 24169356;24846940;26335100;24137477;26686386;24402874;21399871;23585871;24762088;23079745 MiR 125b acts as an oncogene in glioblastoma cells and inhibits cell apoptosis through p53 and p38MAPK independent pathways; Further studies reveal that p53 is regulated by miR-125b; However downregulation of the endogenous miR-125b also results in p53-independent apoptotic pathway leading to apoptosis in p53 mutated U251 cells and p53 knockdown U87 cells;The results revealed that hsa-miR-125b may regulate multiple biological processes and signal transduction pathways and drug-resistant occurrence is associated with cell proliferation cell apoptosis cell cycle and signaling pathways including MAPK Wnt and p53;The carboxy terminal domain of connexin 43 CT Cx43 modulates the expression of p53 by altering miR 125b expression in low grade human breast cancers; In addition CT-Cx43 was exogenously expressed in the breast cancer-derived cell line MCF-7 and its effect on the expression of miR-125b and its downstream target p53 were evaluated as well as its effect on cellular proliferation and death using MTT and LDH assays respectively; Interestingly we found that miR-125b a negative regulator of p53 exhibited an inverse expression relationship with CT-Cx43 in the breast cancer samples tested;TP53 and hsa-miR-125b were observed to form a self-adaptation association;In this study we have investigated the delivery and transfection of wild-type wt- p53 and microRNA-125b miR-125b expressing plasmid DNA in SK-LU-1 human lung adenocarcinoma cells as well as in KrasG12D/p53fl/fl KP genetically engineered mouse model of lung cancer;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;In vitro assays revealed that overexpression of miR-125b repressed the endogenous level of p53 protein in human colorectal cancer cells;In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b;A stable overexpression of miR-125b in human melanoma cell line Mel-Juso resulted in a G0/G1 cell cycle block and emergence of large cells expressing senescence markers: senescence-associated beta-galactosidase p21 p27 and p53;Furthermore treatment of HCC cells with 5-aza-2'-deoxycytidine or ectopic expression of wildtype but not mutated p53 restored miR-125a-5p and miR-125b expression and inhibited tumor cell growth suggesting their regulation by promoter methylation and p53 activity; To show the clinical significance of these findings mutations in the DNA binding domain of p53 and promoter methylation of miR-125b were investigated; Four out of nine patients with induced SIRT7 carried mutations in the p53 gene and one patient showed hypermethylation of the miR-125b promoter region ;;;;;immune resistance;;;; glioblastoma;gastric cancer;breast cancer;pancreatic cancer;lung cancer;cervical and endocervical cancer;colorectal cancer;prostate cancer;melanoma;liver cancer hsa-miR-150-5p TP53 0.0645271721519611 0.513295658828718 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.156281214913021 3.09404029899862e-06 23747308;23228962 miR 150 promotes the proliferation of lung cancer cells by targeting P53; Here we demonstrate that miR-150 is aberrantly upregulated in lung cancer tissue and negatively correlates with the expression of the proapoptotic gene p53 but not EGR2; We show that miR-150 specifically targets the 3'-UTR of p53 and regulates its expression; Inhibition of miR-150 effectively delays cell proliferation and promotes apoptosis accompanied by increased p53 protein expression;miR-150 and miR-3940-5p were found to be significantly downregulated in p53 IHC-positive NSCLC cases and were negatively correlated with p53 mRNA; miR-150 and miR-3940-5p may affect p53 expression through a direct or indirect pathway ; lung cancer;lung squamous cell cancer hsa-miR-221-3p TP53 -0.0109507285071775 0.936440693394519 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.143405645468124 6.89189438710031e-10 20880178;24324033 Circulating miR 221 directly amplified from plasma is a potential diagnostic and prognostic marker of colorectal cancer and is correlated with p53 expression; The correlation between miR-221 levels and protein levels of p53 CEA ER and PR clinicopathological features or overall survival was analyzed; The immunohistochemistry analysis demonstrates a significant correlation between plasma miR-221 level and p53 expression; The direct amplification of plasma miR-221 can be used as a potential noninvasive molecular marker for diagnosis and prognosis of CRC and is correlated with p53 expression;Interestingly miR-221 can activate the p53/mdm2 axis by inhibiting MDM2 and in turn p53 activation contributes to miR-221 enhanced expression; Moreover by modulating the p53 axis miR-221 impacts cell-cycle progression and apoptotic response to doxorubicin in hepatocellular carcinoma-derived cell lines; These data were confirmed in clinical specimens of hepatocellular carcinoma in which elevated miR-221 expression was associated with the simultaneous presence of wild-type p53 and DNA hypomethylation poor survival;worse prognosis;progression;drug resistance colorectal cancer;liver cancer hsa-miR-222-3p TP53 0.107117065575197 0.423818119725697 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.142653482163308 1.2671214916531e-09 NA NA NA hsa-miR-30d-5p TP53 -0.0607010868720046 0.471410043548794 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.336903694979927 3.32137008004591e-09 NA NA NA hsa-miR-324-5p TP53 0.0183370482495953 0.842996782677271 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.170645382602635 1.21583558752455e-06 NA NA NA hsa-miR-338-3p TP53 -0.39963704679384 0.0034410643220691 0.346312114104146 1.42081328137217e-05 miRanda -0.117560999445537 4.2049029410775e-06 NA NA NA hsa-miR-34a-5p TP53 0.143472919835524 0.211283228567057 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.127236433475415 7.74576992902642e-06 23155233;19773441;25572695;25123132;22438124;21702042;22292433;21383543;25982144;19443717;26790955;24642471;22198213;25362853;23036084;19421141;24444609;20186752;22457788;27186405;21731696;19736307;26879132;23569431;25771001;23292869;24209638;24957404;21240262;24503183;22102859;18803879;24630988;25895459;25894979;19714243;26944831;19921694;21909380;25789847;23450486;24402874;24337371;18497571;25490093;22593438;24528540;20145172;23349340;25038915;21321636;23632240;23624843;19029026;20309940;22810507;23862748;25932212;18834855;21225432 The high miR-34a expression level in the cells after irradiation at 60 Gy reduced the p53 expression level;MicroRNA-34a miR-34a is a transcriptional target of p53 that is down-regulated in some cancer cell lines; Analysis of human specimens showed that miR-34a expression is down-regulated in glioblastoma tissues as compared with normal brain and in mutant p53 gliomas as compared with wild-type p53 gliomas;For this purpose cell viability assay Realtime quantitative PCR for mRNA quantification western blot for essential protein expression p53 silencing by shRNA and miR-34a knockdown were performed in the present study; PRE also elevated p53 protein and triggered miR-34a expression;As expected p53 loss caused downregulation of established p53 targets e.g p21 and miR-34 family and increased proliferation in both luminal and basal-like cell lines;Moreover re-expression of miR-34a by transfection in NSCLC cells resulted in inhibition of cell growth and invasiveness induction of apoptosis and enhanced p53 activity;MiR 34a chemosensitizes bladder cancer cells to cisplatin treatment regardless of p53 Rb pathway status; MiR-34a is a downstream effector of p53 that has been shown to target several molecules associated with cell cycle and cell survival pathways; As alterations in these pathways are frequent in muscle invasive transitional cell carcinoma of the bladder MI-TCC for example mutation or loss of p53 and Rb the goal of this study was to determine whether manipulation of miR-34a expression levels could abrogate the effect of these alterations and sensitize bladder cancer cells to chemotherapy;Reporter assay further showed that NF-kappaB-induced miR-34a transcriptional activity was reduced by p53 impairment; And wildtype p53 is responsible for NF-kappaB-mediated miR-34a transcriptional activity but not for NF-kappaB binding;Recently miR-34 family has been shown to be part of the p53 pathway which is frequently involved in lung cancer and the expression of miR-34 has been reported to be regulated by DNA methylation;5 Aminolevulinic acid mediated sonodynamic therapy induces anti tumor effects in malignant melanoma via p53 miR 34a Sirt1 axis; Therefore the p53 miR-34a and SIRT1 constituted a positive feedback loop;miR-34a expression could be increased in Y79 cells but not Weri-Rb1 cells after p53 activation; This differential regulation was not caused by genomic alterations at the miR-34a p53 binding site or mature gene;Conversely kallistatin stimulated expression of the tumorigenic suppressors miR-34a and p53;Members of the miR-34 family are induced by the tumor suppressor p53 and are known to inhibit epithelial-to-mesenchymal transition EMT and therefore presumably suppress the early phases of metastasis;MicroRNA-34a miR-34a a transcriptional target of p53 is a well-known tumor suppressor gene; Moreover we found that miR-34a was downregulated in 25 of 40 62.5% colon cancer tissues as compared with the adjacent normal colon tissues and that the expression of miR-34a was correlated with the DNA-binding activity of p53;Ectopic expression of miR-34a-5p in p53 wild-type colon cancer cell HCT116 significantly inhibited cell growth migration invasion and metastasis; miR-34a-5p induced cell apoptosis cell cycle arrest at G1 phase and p53 transcription activity in HCT116 cells but not in the HCT116 p53 knockout p53-/- cells; miR-34a-5p significantly suppressed the HCT116 growth in vivo whereas it showed no effect on the HCT116 p53-/- xenograft indicating that the growth-inhibiting effect by miR-34a-5p was dependent on p53;microRNA 34a sensitizes lung cancer cell lines to DDP treatment independent of p53 status; However the precise biological role of miR-34a in p53 deficient lung cancer cell lines remains largely elusive; Overall in this study we found the proliferation inhibition function of miR-34a in vitro in lung cancer cell lines is p53 independent and also demonstrated the combination therapeutic potential of miR-34a and DDP in lung cancer cell lines;In particular mammalian miR-34 is upregulated by p53 in response to radiation but little is known about the role of this miRNA in vivo; These findings show a role for mir-34 in both apoptotic and non-apoptotic cell death in vivo much like that of cep-1 the C elegans p53 homolog;Tumor-suppressive miR-34a a direct target of p53 has been shown to target several molecules of cell survival pathways; Here we show that capsaicin-induced oxidative DNA damage culminates in p53 activation to up-regulate expression of miR-34a in non-small cell lung carcinoma NSCLC cells;Accumulating evidence suggests that miR-34a as a key mediator of p53 tumor suppression is aberrantly expressed in human cancers; Bioinformatics analysis produced a protein-protein interaction network which revealed that the p53 signaling pathway and cell cycle pathway were two major hubs containing most of the proteins regulated by miR-34a;Studies have demonstrated that miR-34a which is a direct target of the p53 tumor suppressor gene functions as a tumor suppressor and is associated with the tumor growth and metastasis of various human malignances;Knockdown of oncoprotein E6 expression of human papillomavirus in SiHa and HeLa cells by siRNAs lead to an increased protein level of p53 decreased level of miR-34a as well as reduced Warburg effect;miR-34a is known as a p53 regulated tumor suppressor microRNA in many cancer types;In addition in patients with sufficient tumor tissue we assessed p53 mutations and the methylation status of the MIRN34A gene promoter region and correlated these findings with miR-34a expression; Patients with both p53 mutations and low miR-34a levels had the highest probability of relapse P = 0.001;Particularly miR-34a has been revealed to be a direct transcriptional target of p53 which is frequently mutated in epithelial ovarian carcinomas especially in high grade serous cancer; The aim of this study was to investigate the clinical relevance of mir34a as well as its promoter methylation in a subset of 133 ovarian cancers with a special focus on the p53 mutation status the dualistic type I and type II ovarian cancer model and the different histotypes; The expression of miR-34a was found lower in type II than in type I cancers p = 0.037 in p53 mutated as compared to p53 wild type cancers p = 0.003 and in high grade compared to in low grade cancers p = 0.028; The inverse association between miR-34a expression and grading p53 mutation status and dualistic tumor type classification together with its prognostic relevance may underline the tumor-suppressive character of miR-34a in ovarian cancer;As a direct target gene of p53 miR-34a has been suggested to mediate the tumor suppressor function of p53;Further studies indicated that PEG4000-TQ-Nps could significantly increase the expression of miR-34a through p53;Downregulation of miR 34a in breast tumors is not associated with either p53 mutations or promoter hypermethylation while it correlates with metastasis; Recent studies have shown that p53 upregulates miR-34 family leading to direct repression of several key oncogenes; In this study expression of mature miR-34a in breast tumors with wild-type p53 was investigated in order to find any correlation between dysregulation of miR-34a expression and breast cancer; In about 40 % of the wild-type p53 samples miR-34a was significantly downregulated; This study has provided evidence that miR-34a expression can be affected in a significant proportion of breast tumors independent of p53; Knowledge of miR-34a status may provide additional useful information regarding the nature of breast tumors especially when p53 testing does not show any aberration;Among these we found well studied molecules such as the miR-17-92 cluster comprising potent oncogenic microRNA and miR-34 recently found to interact with p53;The results demonstrated that miR-199a and miR-34a could induce the apoptosis of human osteosarcoma cells via p53 signalling pathway;Here we show through expression analysis that miR-34a a p53 target was underexpressed in CD44+ prostate cancer cells purified from xenograft and primary tumors;Members of the miR-34 family have been shown to be transcriptional targets of the tumour suppressor gene P53;Functional genomic analysis highlighted a novel regulatory role of the transcription factor MAZ apart from the known control by p53 on the expression of miR-34a and a number of miR-34a targets; Our analysis for regulatory loops suggest that MAZ and p53 transcription factors co-operate in modulating miR-34a as well as miR-34a targets involved in several cellular pathways;Restoration of tumor suppressor miR 34 inhibits human p53 mutant gastric cancer tumorspheres; MicroRNA miR-34 was recently found to be a direct target of p53 functioning downstream of the p53 pathway as a tumor suppressor; Our results demonstrate that in p53-deficient human gastric cancer cells restoration of functional miR-34 inhibits cell growth and induces chemosensitization and apoptosis indicating that miR-34 may restore p53 function;miR 34 cooperates with p53 in suppression of prostate cancer by joint regulation of stem cell compartment; The miR-34 family was originally found to be a direct target of p53 and is a group of putative tumor suppressors; Here we report that mice with prostate epithelium-specific inactivation of mir-34 and p53 show expansion of the prostate stem cell compartment and develop early invasive adenocarcinomas and high-grade prostatic intraepithelial neoplasia whereas no such lesions are observed after inactivation of either the mir-34 or p53 genes alone by 15 months of age; Consistently combined deficiency of p53 and miR-34 leads to acceleration of MET-dependent growth self-renewal and motility of prostate stem/progenitor cells; Our study provides direct genetic evidence that mir-34 genes are bona fide tumor suppressors and identifies joint control of MET expression by p53 and miR-34 as a key component of prostate stem cell compartment regulation aberrations in which may lead to cancer;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however the effect of miR-34a on the ovarian cancer cells remains unclear;The microRNA-34 family miR-34a -34b and -34c have been reported to be tumor suppressor microRNAs miRNAs that are regulated by the TP53 and DNA hypermethylation; To elucidate the roles of miR-34 family in colon carcinogenesis miR-34a/b/c were measured in tumors and adjacent noncancerous tissues from 159 American and 113 Chinese colon cancer patients using quantitative RT-PCR and we examined associations between miR-34a/b/c expression with TNM staging cancer-specific mortality TP53 mutation status and Affymetrix microarray data;Transcription of the three miRNA miR-34 family members was recently found to be directly regulated by p53; Our results demonstrate that miR-34 may restore at least in part the tumor suppressing function of the p53 in p53-deficient human pancreatic cancer cells;p53 status was not correlated with miR-34a;The above results suggest that microRNA-34a is one of the important components of PRIMA-1-induced apoptotic network in the cancer cells harboring mutant p53;MicroRNA-34a miR-34a is a transcriptional target of p53 and is down-regulated in pancreatic cancer;Phytochemical regulation of the tumor suppressive microRNA miR 34a by p53 dependent and independent responses in human breast cancer cells; The tumor suppressive microRNA miR-34a is transcriptionally regulated by p53 and shown to inhibit breast cancer cell proliferation as well as being a marker of increased disease free survival; Human breast cancer cells expressing wild-type MCF-7 or mutant p53 T47D were treated with a concentration range and time course of each phytochemical under conditions of cell cycle arrest as detected by flow cytometry to examine the potential connection between miR-34a expression and their anti-proliferative responses; Our results suggest that miR-34a is an essential component of the anti-proliferative activities of I3C artemisinin and artesunate and demonstrate that both wild-type p53 dependent and independent pathways are responsible for miR-34a induction;Compared with p53 normal group the expression level of miR-34a was significantly lower in p53 deletion group;miR-34a is directly regulated by p53 and acts as tumor suppressor while miR-125b plays a significant role in immune response and apoptosis;The microRNA miR-34 family is a direct transcriptional target of tumor-suppressor TP53 and loss of miR-34 function may impair TP53-mediated cell cycle arrest and apoptosis;It appears that AR-dependent inhibition of p53 resulted in suppression of miR-34a and -34c expression;In this study we evaluated the cascade of events determined by etoposide-induced DNA damage in OS cell lines with different p53 status focusing on methylation status and expression of miR-34a that modulate tumor cell growth and cell cycle progression; Our results suggest that the open and unmethylated conformation of the miR-34a gene may be regulated by p53 able to bind the gene promoter;The miR-34 family under-expressed in-non small cell lung cancers NSCLCs are effectors of p53 activation upon irradiation of cells;MiR-34a a direct p53 target gene possesses tumor-suppressive properties as they mediate apoptosis cell cycle arrest and senescence;The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in human epithelial ovarian cancer EOC; miR-34a expression is decreased in 100% and miR-34b*/c in 72% of EOC with p53 mutation whereas miR-34a is also downregulated in 93% of tumors with wild-type p53; Finally miR-34 reconstitution experiments in p53 mutant EOC cells resulted in reduced proliferation motility and invasion the latter of which was dependent on MET expression;miR-34a is transcriptionally induced by the tumor suppressor gene p53 which is often downregulated in non-small cell lung cancer NSCLC;In conclusion overexpression of PEBP4 reduced the sensitivity of A549 cells to DDP-induced cytotoxicity mainly through the altered expression of the p53 protein or the modulation of miR-34a;To investigate the effects of miR-449 and miR-34 on cell growth cell cycle and target gene expression based on these miRNA different expressions in ovarian cancer cell lines SKOV3 and SKOV3-ipl both with mutation of p53;Numerous studies have focused on the association between miR-34 family members which are direct p53 targets and carcinogenesis of many cancers including hepatocellular carcinoma HCC;p53 regulates nuclear GSK 3 levels through miR 34 mediated Axin2 suppression in colorectal cancer cells;MicroRNA-34a miR-34a a potential key effector of the p53 tumor-suppressor gene was studied as a potential tumor suppressor in uveal melanoma;The miR-34 family members are direct transcriptional targets of tumor suppressor p53 and loss of miR-34 function can impair p53-mediated cell cycle arrest and apoptosis;Profiling microRNA miRNA expression delineated TP53 alteration-associated miRNA profiles and identified miR-34a and miR-100 as the most significantly down- and upregulated miRNA respectively; Clinically low miR-34a expression and TP53 alterations predicted for chemotherapy resistance and inferior outcome; Thus detailed molecular profiling links impaired p53 to decreased miR-34a expression but also identifies p53-independent miR-34a induction mechanisms as shown in TP53biallelic altered cell lines treated with 15-deoxy-Δ1214-prostaglandin;MiR-34a a direct target of p53 has been shown to target several molecules associated with the cell cycle and cell survival pathways and its dysregulation is implicated in cancer drug resistance or sensitivity in several human cancers;MicroRNA-34a miR-34a is a direct transcriptional target of p53 and links tumor suppressor function and the oncogenic pathways in some cancers;Tumor suppressor p53 transcriptionally regulates expression of microRNA-34a which confers translational inhibition and mRNA degradation of genes involved in cell cycle control and apoptosis; MiR-34a expression was markedly reduced in p53-null PC3 cells and p53-mutated DU145 cells compared with LNCaP cells expressing wild-type p53;The microRNA encoding genes miR-34a and miR-34b/c represent direct p53 target genes and possess tumor suppressive properties as they mediate apoptosis cell cycle arrest and senescence; In the colorectal cancer samples a statistically significant correlation of miR-34a methylation and the absence of p53 mutation was detected; The mutual exclusiveness of miR-34a methylation and p53 mutation indicates that miR-34a inactivation may substitute for loss of p53 function in cancer ;;;;;poor survival;;;malignant trasformation;;;metastasis;;metastasis;;drug resistance;poor survival;;metastasis;;;;;;;metastasis;;;;;;;motility;;staging;tumorigenesis;;;;;drug resistance;poor survival;;immune resistance;;;progression;;;motility;;;;tumorigenesis;;;;drug resistance;poor survival;drug resistance;;; glioblastoma;glioblastoma;bladder cancer;breast cancer;lung squamous cell cancer;bladder cancer;esophageal cancer;lung squamous cell cancer;melanoma;retinoblastoma;breast cancer;colorectal cancer;colon cancer;colorectal cancer;lung cancer;breast cancer;lung squamous cell cancer;liver cancer;sarcoma;cervical and endocervical cancer;lung squamous cell cancer;lung squamous cell cancer;ovarian cancer;sarcoma;breast cancer;breast cancer;head and neck cancer;sarcoma;prostate cancer;kidney renal cell cancer;breast cancer;gastric cancer;prostate cancer;ovarian cancer;colon cancer;pancreatic cancer;esophageal cancer;lung cancer;pancreatic cancer;breast cancer;B cell lymphoma;cervical and endocervical cancer;colorectal cancer;prostate cancer;sarcoma;lung squamous cell cancer;esophageal cancer;ovarian cancer;lung squamous cell cancer;lung cancer;ovarian cancer;liver cancer;colorectal cancer;melanoma;liver cancer;acute myeloid leukemia;liver cancer;gastric cancer;prostate cancer;sarcoma hsa-miR-380-5p TP53 -0.356387125389103 0.0163515117264043 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate -0.130032370502874 2.53621800466177e-09 NA NA NA hsa-miR-381-3p TP53 -0.192118086401438 0.203198554750071 0.346312114104146 1.42081328137217e-05 MirTarget -0.10958470195975 2.36383687034549e-07 NA NA NA hsa-miR-421 TP53 -0.171573235029879 0.0518112933651029 0.346312114104146 1.42081328137217e-05 miRanda -0.12756767272718 0.000455424352559313 NA NA NA hsa-miR-491-5p TP53 -0.749881113074983 8.22859263179194e-09 0.346312114104146 1.42081328137217e-05 MirTarget;miRanda -0.210461133541589 1.59624493209635e-19 23519249 Targeted site prediction indicated that both Bcl-XL and TP53 contain miR-491-5p recognizing sites in their 3' UTRs; Overexpression of miR-491-5p in the pancreatic cancer cell line SW1990 effectively inhibited both endogenous Bcl-XL and TP53 gene expressions pancreatic cancer hsa-miR-504-5p TP53 -0.510726779625117 6.12122269907368e-05 0.346312114104146 1.42081328137217e-05 miRNAWalker2_validate;MirTarget -0.175599590135581 1.78517568542536e-12 25015107 TFF1 activates p53 through down regulation of miR 504 in gastric cancer; Alternatively we found that the reconstitution of TFF1 down-regulates miR-504 a negative regulator of p53; Western blot analysis data demonstrated that miR-504 abrogates TFF1-induced p53 protein expression and activity; In conclusion the in vitro and in vivo data demonstrate for the first time a novel mechanism by which the tumor suppressor functions of TFF1 involve activation of p53 through down-regulation of miR-504 gastric cancer hsa-miR-129-5p TTK -0.567133990117126 0.00116152215679936 1.68596043296831 1.79039411963732e-22 miRanda -0.238655417869114 1.93024342130145e-07 NA NA NA hsa-miR-139-5p TTK -0.647754756897633 2.15378307237163e-06 1.68596043296831 1.79039411963732e-22 miRanda -0.349272498859988 2.94001110136929e-09 NA NA NA hsa-miR-338-5p TTK -0.424003687569629 0.00246415720301323 1.68596043296831 1.79039411963732e-22 PITA -0.267401682568628 2.38370224047844e-06 NA NA NA hsa-let-7b-3p WEE1 -0.00495618733298731 0.952288980686967 0.985698974997363 4.14674818906967e-22 mirMAP;miRNATAP -0.169620506632819 0.00337501638640001 NA NA NA hsa-let-7g-3p WEE1 0.00917503654783935 0.92786975338911 0.985698974997363 4.14674818906967e-22 MirTarget -0.125129151549055 0.00760618014195701 NA NA NA hsa-miR-1-3p WEE1 -0.218035884810576 0.125196862509753 0.985698974997363 4.14674818906967e-22 MirTarget -0.11369076544329 0.000373151008313687 NA NA NA hsa-miR-103a-3p WEE1 -0.147443193527362 0.128400914654149 0.985698974997363 4.14674818906967e-22 miRNAWalker2_validate -0.236813366234847 0.00269003676410922 NA NA NA hsa-miR-105-5p WEE1 -0.567459149851677 8.20858944634795e-06 0.985698974997363 4.14674818906967e-22 miRNATAP -0.24479516383038 1.02443512046905e-11 NA NA NA hsa-miR-128-3p WEE1 -0.781932174412672 7.1812756321342e-10 0.985698974997363 4.14674818906967e-22 miRNAWalker2_validate;miRTarBase;MirTarget -0.413332363369616 1.06644345449199e-28 NA NA NA hsa-miR-129-2-3p WEE1 -0.6175132197013 0.000561573620904867 0.985698974997363 4.14674818906967e-22 MirTarget -0.142793921377287 1.5064927057037e-08 NA NA NA hsa-miR-129-5p WEE1 -0.567133990117126 0.00116152215679936 0.985698974997363 4.14674818906967e-22 miRanda -0.177636038803994 1.50335734789472e-11 NA NA NA hsa-miR-137 WEE1 -0.924849266740909 1.546832202049e-06 0.985698974997363 4.14674818906967e-22 miRanda -0.166810828043277 4.20780723420529e-13 NA NA NA hsa-miR-139-5p WEE1 -0.647754756897633 2.15378307237163e-06 0.985698974997363 4.14674818906967e-22 miRanda -0.331574814099741 1.15164959267857e-23 NA NA NA hsa-miR-338-3p WEE1 -0.39963704679384 0.0034410643220691 0.985698974997363 4.14674818906967e-22 miRanda -0.226130609893027 4.67170737175624e-10 NA NA NA hsa-miR-508-3p WEE1 -0.292045725850845 0.0158994578296519 0.985698974997363 4.14674818906967e-22 miRNATAP -0.149020533663897 7.31053800777113e-05 NA NA NA hsa-miR-577 WEE1 -0.382068371859275 0.0145513192729119 0.985698974997363 4.14674818906967e-22 PITA -0.139550464428695 2.00959468935901e-06 NA NA NA hsa-miR-320a YWHAG 0.092135599636725 0.243028758989929 -0.0830248224234289 0.29162004243461 PITA;miRanda;miRNATAP -0.199400054484365 9.98164764271041e-06 NA NA NA hsa-miR-320c YWHAG 0.343259124189966 0.000248338401291051 -0.0830248224234289 0.29162004243461 PITA;miRanda;mirMAP;miRNATAP -0.137906277834443 9.70409559717807e-06 NA NA NA hsa-miR-500a-5p YWHAG 0.0634452729978863 0.396072382601677 -0.0830248224234289 0.29162004243461 MirTarget;miRNATAP -0.10181422496234 0.00820391735977638 NA NA NA hsa-miR-320a YWHAH 0.092135599636725 0.243028758989929 -0.0972709573045032 0.288940532234666 MirTarget;PITA;miRanda -0.254691930624819 1.00171465302225e-05 NA NA NA hsa-miR-320c YWHAH 0.343259124189966 0.000248338401291051 -0.0972709573045032 0.288940532234666 MirTarget;PITA;miRanda -0.134544326840746 0.000765166401614011 NA NA NA hsa-miR-9-3p YWHAH -0.184945845984695 0.0290051908009009 -0.0972709573045032 0.288940532234666 MirTarget -0.234786793332634 5.94557089030069e-05 NA NA NA