miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-106b-5p APC 0.648901865047099 8.24870862047885e-09 -0.178106776451097 0.0679159506996304 miRNAWalker2_validate;miRTarBase -0.152406181389829 0.000242059519728295 23087084 miR 106b downregulates adenomatous polyposis coli and promotes cell proliferation in human hepatocellular carcinoma; Moreover we demonstrated that miR-106b downregulates APC expression by directly targeting the 3'-untranslated region of APC messenger RNA; Taken together our results suggest that miR-106b plays an important role in promoting the proliferation of human hepatoma cells and presents a novel mechanism of micro RNA-mediated direct suppression of APC expression in cancer cells liver cancer hsa-miR-21-5p APC 1.51084295924322 1.54551622173259e-34 -0.178106776451097 0.0679159506996304 miRNAWalker2_validate -0.170963654395431 1.15664392926358e-06 23773491;24832083 The prognostic significance of APC gene mutation and miR 21 expression in advanced stage colorectal cancer; The aim of this study was to analyse the association of APC gene mutation and miR-21 expression with clinical outcome in CRC patients; APC gene mutation and expression of APC and miR-21 were analysed by direct DNA sequencing and real-time reverse transcription polymerase chain reaction; APC gene expression was low in CRC and negatively correlated with miR-21 expression and gene mutation; In Taiwan downregulation of the APC gene in CRC correlated with gene mutation and miR-21 upregulation; APC mutation and miR-21 expression could be used to predict the clinical outcome of CRC especially in patients with advanced disease;MicroRNA 21 promotes tumour malignancy via increased nuclear translocation of β catenin and predicts poor outcome in APC mutated but not in APC wild type colorectal cancer; However in our preliminary data the prognostic value of miR-21 levels was observed only in adenomatous polyposis coli APC-mutated tumours not in APC-wild-type tumours; We enrolled 165 colorectal tumour to determine APC mutation miR-21 levels and nuclear β-catenin expression by direct sequencing real-time PCR and immunohistochemistry staging; colorectal cancer;colorectal cancer hsa-miR-103a-3p ARHGAP5 0.765568928457606 3.05497476509383e-12 -0.390501975399923 8.01729872271933e-05 MirTarget -0.190378797120845 9.62393438802819e-06 NA NA NA hsa-miR-155-5p ARHGAP5 0.0108524225071784 0.95651331033505 -0.390501975399923 8.01729872271933e-05 mirMAP -0.106689067532435 1.07117354005012e-05 NA NA NA hsa-miR-15a-5p ARHGAP5 0.349521211668244 0.000770735998804736 -0.390501975399923 8.01729872271933e-05 MirTarget;miRNATAP -0.140114967228855 0.00276448285918912 NA NA NA hsa-miR-16-1-3p ARHGAP5 0.392360028038559 0.00112481918108198 -0.390501975399923 8.01729872271933e-05 MirTarget -0.10655854170515 0.00823158833704956 NA NA NA hsa-miR-17-5p ARHGAP5 0.698861147045461 2.43854106455398e-05 -0.390501975399923 8.01729872271933e-05 miRNAWalker2_validate -0.168233712468027 4.06938935137717e-09 NA NA NA hsa-miR-181a-5p ARHGAP5 0.250113435902954 0.0551933249319293 -0.390501975399923 8.01729872271933e-05 mirMAP -0.178285338979484 1.42745874554536e-06 NA NA NA hsa-miR-181b-5p ARHGAP5 0.488256489401108 0.00105321129419701 -0.390501975399923 8.01729872271933e-05 mirMAP -0.156486821983914 1.13906378427161e-06 NA NA NA hsa-miR-18a-5p ARHGAP5 0.918028187958706 1.90229510425322e-05 -0.390501975399923 8.01729872271933e-05 MirTarget -0.139657415536736 1.92972267421708e-10 NA NA NA hsa-miR-19a-3p ARHGAP5 1.01712596153672 2.57753201450625e-07 -0.390501975399923 8.01729872271933e-05 miRNATAP -0.102176278799659 2.18507337800794e-05 NA NA NA hsa-miR-19b-3p ARHGAP5 0.601279551368824 0.00016685097085251 -0.390501975399923 8.01729872271933e-05 miRNATAP -0.126249258755046 2.62351845075989e-05 NA NA NA hsa-miR-421 ARHGAP5 0.941856334694081 6.12167297054077e-08 -0.390501975399923 8.01729872271933e-05 PITA;miRanda;mirMAP;miRNATAP -0.100369042715833 0.000260906838220345 NA NA NA hsa-miR-500a-5p ARHGAP5 0.797906382969156 9.15958171084226e-08 -0.390501975399923 8.01729872271933e-05 mirMAP -0.130200028078366 4.44416835178627e-05 NA NA NA hsa-miR-501-5p ARHGAP5 1.15231083477882 2.98906150004088e-10 -0.390501975399923 8.01729872271933e-05 PITA;mirMAP -0.135613237661836 1.27605396391392e-07 NA NA NA hsa-miR-590-5p ARHGAP5 -0.103355559980404 0.310027295317092 -0.390501975399923 8.01729872271933e-05 miRanda -0.125900328591526 0.00896894861120512 NA NA NA hsa-let-7b-3p AXIN2 -1.21979213547786 5.84575581584603e-17 0.113841868770703 0.752977397700815 miRNATAP -0.270257334726793 0.0204955473660487 NA NA NA hsa-let-7g-3p AXIN2 -1.14091000722297 8.32736018188759e-19 0.113841868770703 0.752977397700815 MirTarget -0.333163518873582 0.0112609172880632 NA NA NA hsa-miR-15b-5p AXIN2 0.230566941413564 0.0824765001857479 0.113841868770703 0.752977397700815 miRTarBase;MirTarget;miRNATAP -0.445214007102522 0.000810754926318775 NA NA NA hsa-miR-16-2-3p AXIN2 -0.0336420826463706 0.805158952566973 0.113841868770703 0.752977397700815 mirMAP -0.637896819806337 6.99346379238779e-07 NA NA NA hsa-miR-16-5p AXIN2 -0.398577235179603 0.000101598495180191 0.113841868770703 0.752977397700815 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.492511513287746 0.00421170477125135 NA NA NA hsa-miR-195-3p AXIN2 -1.09375690196245 4.70092324819807e-09 0.113841868770703 0.752977397700815 mirMAP -0.313128484413814 0.000828518151480385 NA NA NA hsa-miR-195-5p AXIN2 -1.85907534356434 1.47434041676402e-22 0.113841868770703 0.752977397700815 MirTarget;miRNATAP -0.245874041969712 0.00506110437798971 NA NA NA hsa-miR-26b-3p AXIN2 -1.25503731613309 4.45390890119649e-27 0.113841868770703 0.752977397700815 MirTarget -0.591476065389494 2.94301063245798e-05 NA NA NA hsa-miR-326 AXIN2 -1.881935132554 1.68087316863775e-21 0.113841868770703 0.752977397700815 miRanda -0.362520003418692 1.98590653589597e-05 NA NA NA hsa-miR-338-5p AXIN2 -0.221898432884726 0.252390988727086 0.113841868770703 0.752977397700815 miRNATAP -0.342154956032422 0.000172654921949776 NA NA NA hsa-miR-424-5p AXIN2 -2.62653257498508 2.38997974737991e-50 0.113841868770703 0.752977397700815 MirTarget;miRNATAP -0.191832991796132 0.0301808709524209 NA NA NA hsa-miR-497-5p AXIN2 -1.41371173021948 7.09278946477743e-17 0.113841868770703 0.752977397700815 MirTarget;miRNATAP -0.294042209891213 0.00328690230713159 NA NA NA hsa-miR-616-3p AXIN2 -0.699516741506971 1.82225985970292e-05 0.113841868770703 0.752977397700815 MirTarget -0.422833368838949 7.60561180413777e-05 NA NA NA hsa-let-7b-5p BIRC5 -0.959013717270803 5.95127281258643e-11 4.49515004999251 9.61981227851246e-50 miRNAWalker2_validate -0.714894665750299 6.76492235439612e-11 NA NA NA hsa-miR-101-3p BIRC5 -1.48176407980989 3.98950707385704e-31 4.49515004999251 9.61981227851246e-50 miRNAWalker2_validate -1.3265120823297 7.97415084582345e-32 NA NA NA hsa-miR-10a-5p BIRC5 -1.47723516092033 7.27299669108673e-16 4.49515004999251 9.61981227851246e-50 miRNAWalker2_validate -0.713644309178663 4.2990229259411e-17 NA NA NA hsa-miR-203a-3p BIRC5 -1.34362479226226 8.69374456831293e-05 4.49515004999251 9.61981227851246e-50 miRTarBase -0.125858297754976 0.00935408966011575 22713668;27714672 Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets; Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells; Moreover up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor;miR 203 is a predictive biomarker for colorectal cancer and its expression is associated with BIRC5; The purpose of this study was to explore the role of miR-203 in colorectal cancer CRC and evaluate the correlation between miR-203 and BIRC5; Finally miR-203 expression was negatively associated with that of BIRC5 r = -0.8150 P < 0.05 ; breast cancer;colorectal cancer hsa-miR-218-5p BIRC5 -0.500091158894849 0.0398595700240766 4.49515004999251 9.61981227851246e-50 miRTarBase;MirTarget -0.141730591808928 0.0393259754882198 25473903;25900794;26442524 Survivin BIRC5 was subsequently identified as an important cervical cancer target of miR-218 using in silico prediction mRNA profiling and quantitative real-time PCR qRT-PCR;miR-218 binds survivin BIRC5 mRNA 3'-UTR and down-regulated reporter luciferase activity;MiR-218 promoted apoptosis inhibited cell proliferation and caused cell cycle arrest in CRC cells by suppressing BIRC5 expression; In conclusion we demonstrated that high miR-218 expression had a positive prognostic value in 5-FU-based treatments for CRC patients and discovered a novel mechanism mediated by miR-218 to promote apoptosis and to function synergistically with 5-FU to promote chemosensitivity by suppressing BIRC5 and TS in CRC ;; cervical and endocervical cancer;breast cancer;colorectal cancer hsa-miR-30c-5p BIRC5 -0.432111642767632 0.000159764714931007 4.49515004999251 9.61981227851246e-50 miRNAWalker2_validate -0.45333717178008 0.00191124919706276 NA NA NA hsa-miR-335-5p BIRC5 -1.61347836513558 1.62757083289711e-19 4.49515004999251 9.61981227851246e-50 miRNAWalker2_validate;MirTarget -0.501439997555754 1.3266718457985e-08 23232114 Genetic variation in a miR 335 binding site in BIRC5 alters susceptibility to lung cancer in Chinese Han populations; In support of the postulation that the 3' UTR SNP may directly affect miRNA-binding site reporter gene assays indicated BIRC5 was a direct target of miR-335 and the rs2239680 T>C change resulted in altered regulation of BIRC5 expression; Our findings defined a 3' UTR SNP in human BIRC5 oncogene that may increase individual susceptibility to lung cancer probably by attenuating the interaction between miR-335 and BIRC5 lung cancer hsa-miR-542-3p BIRC5 -1.31351015149842 3.64642423288576e-22 4.49515004999251 9.61981227851246e-50 miRNAWalker2_validate;MirTarget;miRanda -0.82408325426437 5.64539118201001e-13 NA NA NA hsa-miR-139-5p BUB1 -2.11299531796557 8.16407076415038e-28 4.04762666744344 9.60341711300894e-48 miRanda -0.981222586322315 6.07995380621313e-51 NA NA NA hsa-miR-199a-5p BUB1 -1.98848462405923 2.0421622713753e-11 4.04762666744344 9.60341711300894e-48 miRanda -0.291631300567174 3.71128271880203e-09 NA NA NA hsa-miR-542-3p BUB1 -1.31351015149842 3.64642423288576e-22 4.04762666744344 9.60341711300894e-48 miRanda -0.668988733252235 2.14129391171968e-10 NA NA NA hsa-miR-142-3p BUB1B -1.4243101967815 1.92609481097765e-14 3.85682830100864 2.02974032557381e-43 miRanda -0.198560838022744 0.0119274709130208 NA NA NA hsa-miR-192-5p BUB1B -0.50188499512787 0.00344713341196035 3.85682830100864 2.02974032557381e-43 miRNAWalker2_validate -0.221905021279395 0.0119217114033508 NA NA NA hsa-miR-193b-3p BUB1B -0.166697207281748 0.272022543069482 3.85682830100864 2.02974032557381e-43 miRNAWalker2_validate -0.199232903598723 0.0474370924329797 NA NA NA hsa-miR-215-5p BUB1B -0.981544773246509 3.14452272477156e-05 3.85682830100864 2.02974032557381e-43 miRNAWalker2_validate -0.164346842914509 0.0099257038268172 NA NA NA hsa-miR-22-3p BUB1B -0.628561621952407 1.33755558015523e-09 3.85682830100864 2.02974032557381e-43 miRNAWalker2_validate -1.59565755962297 8.34276186949843e-33 NA NA NA hsa-miR-486-5p BUB1B -1.77766890600022 2.9071717929922e-14 3.85682830100864 2.02974032557381e-43 miRanda -0.415413021317956 1.24965935132642e-11 NA NA NA hsa-let-7a-3p CCNA2 -0.568717557554068 1.00947202557819e-07 3.36794922367745 6.53886370047174e-43 MirTarget -0.508034164420555 3.65278492221913e-05 NA NA NA hsa-let-7b-3p CCNA2 -1.21979213547786 5.84575581584603e-17 3.36794922367745 6.53886370047174e-43 MirTarget -0.732605840868638 4.54728817669008e-18 NA NA NA hsa-let-7b-5p CCNA2 -0.959013717270803 5.95127281258643e-11 3.36794922367745 6.53886370047174e-43 miRNAWalker2_validate;miRTarBase -0.524638048172729 1.97837367658696e-09 NA NA NA hsa-let-7f-1-3p CCNA2 -0.704017923073338 7.84883628944999e-07 3.36794922367745 6.53886370047174e-43 MirTarget -0.411800166097723 9.61755775465089e-06 NA NA NA hsa-miR-130a-3p CCNA2 -1.53229181736223 2.91460363624872e-19 3.36794922367745 6.53886370047174e-43 miRNATAP -0.393381177440701 9.03577520347484e-08 NA NA NA hsa-miR-199a-5p CCNA2 -1.98848462405923 2.0421622713753e-11 3.36794922367745 6.53886370047174e-43 miRanda -0.274665722447149 1.39754779993059e-10 NA NA NA hsa-miR-22-3p CCNA2 -0.628561621952407 1.33755558015523e-09 3.36794922367745 6.53886370047174e-43 MirTarget -1.21746436700899 3.56641376559019e-24 25596928 The sequence of miR-22 which is conserved in mice rats humans and other mammalians aligns with the sequence of 3'-UTR of CCNA2; Chenodeoxycholic acid treatment and miR-22 mimics reduced CCNA2 protein and increased the number of G0/G1 Huh7 and HCT116 cells; In humans the expression levels of miR-22 and CCNA2 are inversely correlated in liver and colon cancers liver cancer hsa-miR-27b-3p CCNA2 -0.823491817819754 9.20935302746823e-14 3.36794922367745 6.53886370047174e-43 miRNATAP -0.54120082992627 3.46716161944693e-06 NA NA NA hsa-miR-29a-3p CCNA2 -0.856762511178136 4.65330336378459e-12 3.36794922367745 6.53886370047174e-43 MirTarget -0.878689289923181 3.31167697738131e-18 NA NA NA hsa-miR-29b-1-5p CCNA2 -0.538946836809295 0.00102913307251186 3.36794922367745 6.53886370047174e-43 MirTarget -0.331466426463458 3.78367154695011e-05 NA NA NA hsa-miR-29b-3p CCNA2 -0.348885528720517 0.0121441341291285 3.36794922367745 6.53886370047174e-43 MirTarget -0.589199607558879 3.67803340321416e-10 NA NA NA hsa-miR-29c-3p CCNA2 -1.4367517608807 1.61767774905781e-21 3.36794922367745 6.53886370047174e-43 MirTarget -0.922743011617596 1.63915073620724e-32 NA NA NA hsa-miR-374b-5p CCNA2 -0.307538028546424 0.0030079617545972 3.36794922367745 6.53886370047174e-43 mirMAP -0.32681314307052 0.0114301592113977 NA NA NA hsa-miR-486-5p CCNA2 -1.77766890600022 2.9071717929922e-14 3.36794922367745 6.53886370047174e-43 miRanda -0.275730733902838 4.10950790707083e-07 NA NA NA hsa-let-7b-5p CCNB1 -0.959013717270803 5.95127281258643e-11 3.15540953438863 5.73583669464518e-46 miRNAWalker2_validate -0.536702047843919 1.21768540248182e-11 NA NA NA hsa-miR-139-5p CCNB1 -2.11299531796557 8.16407076415038e-28 3.15540953438863 5.73583669464518e-46 miRanda -0.800903870286373 3.33153076219979e-55 NA NA NA hsa-miR-142-5p CCNB1 -1.44575553250033 1.18797044680592e-13 3.15540953438863 5.73583669464518e-46 MirTarget -0.146430294535015 0.0152842140616675 NA NA NA hsa-miR-192-5p CDC20 -0.50188499512787 0.00344713341196035 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.257887721839452 0.00747315576024174 NA NA NA hsa-miR-215-5p CDC20 -0.981544773246509 3.14452272477156e-05 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.210290018213749 0.00250187708869541 NA NA NA hsa-miR-23b-3p CDC20 -0.530561587109023 5.43807206823652e-07 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.727746315213922 2.45876854567768e-06 NA NA NA hsa-miR-30a-5p CDC20 -0.626257932887855 0.000108198196973765 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.686824996889202 5.1373930712059e-12 NA NA NA hsa-miR-29c-3p CDC23 -1.4367517608807 1.61767774905781e-21 0.463635495366116 5.65757358389337e-10 miRNAWalker2_validate -0.137258097204054 2.34594396923131e-09 NA NA NA hsa-miR-146b-5p CDH1 0.419719546795449 0.0457375823220155 -0.925620688902025 3.54334396234e-05 miRanda -0.209827116364557 5.56204266800899e-05 NA NA NA hsa-miR-185-5p CDH1 0.480006257154717 6.35758891340152e-07 -0.925620688902025 3.54334396234e-05 MirTarget -0.447644587025903 7.92400318750844e-05 27212161;24025511 We also found that ectopic expression of miR-185 reversed EMT via the up-regulation of E-cadherin and down-regulation of vimentin in epithelial and mesenchymal HCC cells;After transfection of miR-185 inhibitor into KYSE30 the expression of E-cadherin a downstream protein of Six1 was observed under confocal microscope; After transfection of miR-185 inhibitorthe expression level of E-cadherin decreased ; liver cancer;esophageal cancer hsa-miR-186-5p CDH1 -0.060103391373687 0.535288717081205 -0.925620688902025 3.54334396234e-05 mirMAP -0.271690734682459 0.0182700262180118 NA NA NA hsa-miR-616-5p CDH1 0.150569904638223 0.402836556304524 -0.925620688902025 3.54334396234e-05 MirTarget;mirMAP -0.142138556800114 0.0208629153342258 NA NA NA hsa-miR-139-5p CDK1 -2.11299531796557 8.16407076415038e-28 3.60092865588574 2.53758251358007e-48 miRanda -0.838938956041355 1.17302084660509e-46 NA NA NA hsa-miR-193b-3p CDK1 -0.166697207281748 0.272022543069482 3.60092865588574 2.53758251358007e-48 miRNAWalker2_validate -0.247245044417844 0.00581826116259039 NA NA NA hsa-miR-195-3p CDK1 -1.09375690196245 4.70092324819807e-09 3.60092865588574 2.53758251358007e-48 MirTarget -0.373417267400659 1.70004237381144e-07 NA NA NA hsa-miR-378a-5p CDK1 -1.58667444700188 3.19640857320504e-17 3.60092865588574 2.53758251358007e-48 MirTarget -0.547488328475554 2.76743919236473e-16 NA NA NA hsa-miR-486-5p CDK1 -1.77766890600022 2.9071717929922e-14 3.60092865588574 2.53758251358007e-48 miRanda -0.358602044004862 6.63525634192938e-11 NA NA NA hsa-miR-139-5p CDK7 -2.11299531796557 8.16407076415038e-28 0.491707717966495 7.16261478943003e-09 miRanda -0.14310504808171 4.57209013484235e-13 NA NA NA hsa-miR-542-3p CDK7 -1.31351015149842 3.64642423288576e-22 0.491707717966495 7.16261478943003e-09 miRanda -0.177460362938327 8.63098208373725e-10 NA NA NA hsa-miR-590-3p CDK7 -0.473167498047808 1.76652934637227e-05 0.491707717966495 7.16261478943003e-09 miRanda -0.178615786936345 2.1764183873582e-06 NA NA NA hsa-miR-181a-5p CDKN1B 0.250113435902954 0.0551933249319293 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase -0.134068475887496 1.13890102336643e-05 NA NA NA hsa-miR-221-3p CDKN1B 1.12244756358612 3.48370308238012e-13 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.153438831523776 5.56071406720925e-10 20146005;23637992;19953484;23939688;19126397;23967190;17569667;22992757;17721077;20461750 Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression staging;tumorigenesis;progression;;;;;;;;poor survival liver cancer;breast cancer;glioblastoma;breast cancer;melanoma;pancreatic cancer;prostate cancer;breast cancer;glioblastoma;ovarian cancer hsa-miR-222-3p CDKN1B 1.08574156066353 1.76407192348131e-11 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.140430207935426 3.56247154847449e-09 19953484;26912358;24895988;24137356;17569667;27282281;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Besides microvesicle marker characterization we evidenced that miR-222 exosomal expression mostly reflected its abundance in the cells of origin correctly paralleled by repression of its target genes such as p27Kip1 and induction of the PI3K/AKT pathway thus confirming its functional implication in cancer;MiR-222 plays an important role in the tumorigenesis of CC possibly by specifically down-regulating p27Kip1 and PTEN;miR 222 is upregulated in epithelial ovarian cancer and promotes cell proliferation by downregulating P27kip1; miR-222 upregulation induced an enhancement of ovarian cancer cell proliferation potential possibly by downregulating its target P27Kip1; A bioinformatic analysis showed that the 3'-UTR of the P27Kip1 mRNA contained a highly-conserved putative miR-222 binding site; Luciferase reporter assays demonstrated that P27Kip1 was a direct target of miR-222; Consistently there was an inverse correlation between the P27Kip1 and miR-222 expression levels in the ovarian cancer cell lines and tissues;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;miR 222 confers the resistance of breast cancer cells to Adriamycin through suppression of p27kip1 expression; Immunofluorescence showed that miR-222 altered the subcellular location of p27kip1 in nucleus; The results showed that downregulation of miR-222 in MCF-7/Adr increased sensitivity to Adr and Adr-induced apoptosis and arrested the cells in G1 phase accompanied by more expressions of p27kip1 especially in nucleus; Taken together the results found that miR-222 induced Adr-resistance at least in part via suppressing p27kip1 expression and altering its subcellular localization and miR-222 inhibitors could reverse Adr-resistance of breast cancer cells;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;tumorigenesis;;;drug resistance;poor survival glioblastoma;melanoma;cervical and endocervical cancer;ovarian cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-24-3p CDKN1B -0.257825594021966 0.00689742600215061 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRNATAP -0.125575697425242 0.00293958904599202 26847530;26044523 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated;With the bioinformatic method we further identified that p27Kip1 is a direct target of miR-24-3p and its protein level was negatively regulated by miR-24-3p ; prostate cancer;breast cancer hsa-miR-98-5p CDKN1B -0.0487189107419432 0.715911066732548 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate -0.150589087046664 3.98220273478164e-07 NA NA NA hsa-miR-107 CDKN2B 0.241662461253461 0.0170788899505389 1.37191742600332 3.8601745589837e-15 PITA -0.22214715039643 0.0113151536408877 NA NA NA hsa-miR-126-5p CDKN2B -0.428561469405594 6.6035585844222e-05 1.37191742600332 3.8601745589837e-15 mirMAP -0.331524443678709 4.86520309619025e-05 NA NA NA hsa-miR-144-3p CDKN2B -2.97536914478815 6.00546047309683e-28 1.37191742600332 3.8601745589837e-15 MirTarget -0.165363817374004 2.99908004062454e-08 NA NA NA hsa-miR-382-5p CDKN2B -0.71884748690977 0.00849172229801356 1.37191742600332 3.8601745589837e-15 mirMAP -0.101243601276092 0.00159366073719854 NA NA NA hsa-miR-450b-5p CDKN2B -1.34333360369432 2.68386079790462e-17 1.37191742600332 3.8601745589837e-15 mirMAP -0.177653728760579 0.000849213139863512 NA NA NA hsa-miR-451a CDKN2B -2.1025161666445 6.82555767537341e-17 1.37191742600332 3.8601745589837e-15 MirTarget -0.162386705840671 1.05027558906518e-06 NA NA NA hsa-miR-486-5p CDKN2B -1.77766890600022 2.9071717929922e-14 1.37191742600332 3.8601745589837e-15 miRanda -0.19221239071621 9.16912999787263e-08 NA NA NA hsa-miR-450b-5p CDKN2C -1.34333360369432 2.68386079790462e-17 2.08821471271675 1.38541951717446e-30 miRNATAP -0.407319031602086 4.73616061228169e-13 NA NA NA hsa-miR-542-3p CDKN2C -1.31351015149842 3.64642423288576e-22 2.08821471271675 1.38541951717446e-30 miRanda -0.51609932750582 1.59421631749972e-15 NA NA NA hsa-miR-15a-5p CHUK 0.349521211668244 0.000770735998804736 -0.19932080126218 0.00863163957240649 miRNAWalker2_validate;miRTarBase;MirTarget -0.122536038974783 0.000565424625908096 NA NA NA hsa-miR-15b-5p CHUK 0.230566941413564 0.0824765001857479 -0.19932080126218 0.00863163957240649 MirTarget -0.118017359725558 2.06238744655946e-05 NA NA NA hsa-miR-16-5p CHUK -0.398577235179603 0.000101598495180191 -0.19932080126218 0.00863163957240649 miRNAWalker2_validate;miRTarBase;MirTarget -0.150607357209835 2.62400382273556e-05 NA NA NA hsa-miR-23a-3p CHUK -0.178875974460574 0.135978273540604 -0.19932080126218 0.00863163957240649 MirTarget -0.100438227795724 0.00114468004476388 NA NA NA hsa-miR-339-5p CHUK 0.280589919878422 0.0355660282647215 -0.19932080126218 0.00863163957240649 miRanda -0.12221351609504 8.92727609693918e-06 NA NA NA hsa-miR-590-5p CHUK -0.103355559980404 0.310027295317092 -0.19932080126218 0.00863163957240649 miRanda -0.11766978557823 0.00128889069327197 NA NA NA hsa-miR-330-3p CTNNB1 -0.332364259424489 0.0316051913061459 0.00180452674840392 0.983564078864382 MirTarget;PITA;miRNATAP -0.128940103294471 3.76576237893285e-06 NA NA NA hsa-miR-331-3p CTNNB1 -0.283974346994635 0.0373785411053202 0.00180452674840392 0.983564078864382 miRNAWalker2_validate -0.113207881387487 0.000273720190926097 NA NA NA hsa-miR-27a-3p CUL1 -0.370807380415112 0.00875721956202713 -0.117901782494851 0.10228448838388 miRNAWalker2_validate -0.100449098638373 4.43211535246804e-05 NA NA NA hsa-miR-30b-5p CUL2 -0.54056186708617 2.44688760798341e-05 0.221493309659475 0.000775493336759859 MirTarget;mirMAP;miRNATAP -0.104130690012811 2.59410967342164e-05 NA NA NA hsa-miR-146a-5p EGFR -0.742958238440575 0.000765983177482633 -1.01672920195188 6.69873186671412e-07 miRNAWalker2_validate -0.224421920698198 6.22524592924969e-07 24895573;25242818;22161865;25596948;25417703;23555954;24839931;21632853 Furthermore western blot showed that miR-146a mimic downregulated EGFR ERK1/2 and stat5 signalings; These effects were less potent compared to that of a siRNA targeting EGFR a known target gene of miR-146a; Moreover miR-146a mimic could enhance the cell growth inhibition and apoptosis induction impact of various EGFR targeting agents;Interestingly re-expression of miR-146a inhibited the invasive capacity of Colo357 and Panc-1 PC cells with concomitant down-regulation of EGFR and IRAK-1; Most importantly we found that the treatment of PC cells with "natural agents" 33'-diinodolylmethane DIM or isoflavone led to an increase in the expression of miR-146a and consequently down-regulated the expression of EGFR MTA-2 IRAK-1 and NF-κB resulting in the inhibition of invasion of Colo357 and Panc-1 cells;MiR 146a suppresses tumor growth and progression by targeting EGFR pathway and in a p ERK dependent manner in castration resistant prostate cancer; Mechanistic studies revealed that miR-146a repressed the expression of EGFR through binding to its 3'-untranslated region; Our findings suggest that ubiquitous loss of miR-146a is a critical mechanism for overexpression of EGFR in CRPC which is crucial to better understanding the pathogenesis of CRPC;Furthermore the expressions of bax and cleaved-caspase-3 mainly were increased in control and overexpression miR-146a groups however the expression of EGFR was inverse; All the results demonstrated that quercetin exhibited excellent effect on inhibiting cell proliferation in human breast cancer cells which was performed by up-regulating miR-146a expression then via inducing apoptosis through caspase-3 activation and mitochondrial-dependent pathways and inhibiting invasion through down-regulating the expression of EGFR;Here we report a previously unrecognized posttranscriptional mechanism by which BRCA1 regulates EGFR expression through the induction of miR-146a; We show that BRCA1 binds to MIR146A promoter and activates transcription which in turn attenuates EGFR expression;In functional experiments miR-146a suppressed cell growth induced cellular apoptosis and inhibited EGFR downstream signaling in five NSCLC cell lines H358 H1650 H1975 HCC827 and H292 miR-146a also inhibited the migratory capacity of these NSCLC cells; On the other hand miR-146a enhanced the inhibition of cell proliferation by drugs targeting EGFR including both TKIs gefitinib erlotinib and afatinib and a monoclonal antibody cetuximab; Our results suggest that these effects of miR-146a are due to its targeting of EGFR and NF-κB signaling;Deregulation of miR 146a expression in a mouse model of pancreatic cancer affecting EGFR signaling; Treatment of PC cells with CDF a novel synthetic compound led to re-expression of miR-146a resulting in the down-regulation of EGFR expression; Further knock-down of miR-146a in AsPC-1 cells led to the up-regulation of EGFR expression and showed increased clonogenic growth; In addition knock-down of EGFR by EGFR siRNA transfection of parental AsPC-1 cells and AsPC-1 cells stably transfected with pre-miR-146a resulted in decreased invasive capacity which was further confirmed by reduced luciferase activity in cells transfected with pMIR-Luc reporter vector containing miR-146a binding site; Collectively these results suggest that the loss of expression of miR-146a is a fundamental mechanism for over-expression of EGFR signaling and that re-expression of miR-146a by CDF treatment could be useful in designing personalized strategy for the treatment of human PC;The regulation of EGFR and IRAK1 by miR-146a was examined with miR-146a-transfected gastric cancer cells; Ectopic expression of miR-146a inhibited migration and invasion and downregulated EGFR and IRAK1 expression in gastric cancer cells; MiR-146a targeting of EGFR and IRAK1 is an independent prognostic factor in gastric cancer cases ;;progression;;;;; liver cancer;pancreatic cancer;prostate cancer;breast cancer;breast cancer;lung squamous cell cancer;pancreatic cancer;gastric cancer hsa-miR-146b-5p EGFR 0.419719546795449 0.0457375823220155 -1.01672920195188 6.69873186671412e-07 miRNATAP -0.221138858293073 3.44879306513365e-06 NA NA NA hsa-miR-148b-5p EGFR 0.295777213389605 0.0255749859535438 -1.01672920195188 6.69873186671412e-07 mirMAP -0.233539600600063 0.00227279523782391 NA NA NA hsa-miR-155-5p EGFR 0.0108524225071784 0.95651331033505 -1.01672920195188 6.69873186671412e-07 miRNAWalker2_validate;MirTarget -0.128466970088693 0.0115801509528144 NA NA NA hsa-miR-17-5p EGFR 0.698861147045461 2.43854106455398e-05 -1.01672920195188 6.69873186671412e-07 TargetScan -0.161590563481239 0.00764134099999989 NA NA NA hsa-miR-188-5p EGFR 1.11646899351496 9.56686958408598e-11 -1.01672920195188 6.69873186671412e-07 mirMAP -0.300235014857475 1.22424681244032e-07 NA NA NA hsa-miR-21-5p EGFR 1.51084295924322 1.54551622173259e-34 -1.01672920195188 6.69873186671412e-07 miRNAWalker2_validate;miRTarBase -0.440371363258275 3.21604130567409e-09 24198203;20113523;24012640;24331411;26563758;19597153;26026961;20048743 In radically resected NSCLC patients the expression levels of miR-21 10b in patients with EGFR mutation were much higher than those without mutation;Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;Further the expression of miR-21 is regulated by EGFR via the activation of β-catenin and AP-1; These data indicate that a feedback loop exists between miR-21 and EGFR; These results clarify a novel association between miR-21 and EGFR in the regulation of cancer cell progression;MiR 21 overexpression is associated with acquired resistance of EGFR TKI in non small cell lung cancer;Higher expression levels of miR-21 AmiR-27a and miR-218 detected in this study suggest potential roles of these miRNAs in primary resistance to EGFR-TKI in advanced NSCLC patients with EGFR exon 19 deletion mutations;MiR 21 is an EGFR regulated anti apoptotic factor in lung cancer in never smokers; The changes in expression of some of these miRNAs including miR-21 were more remarkable in cases with EGFR mutations than in those without these mutations; In the never-smoker-derived lung adenocarcinoma cell line H3255 with mutant EGFR and high levels of p-EGFR and miR-21 antisense inhibition of miR-21 enhanced AG1478-induced apoptosis; In a never-smoker-derived adenocarcinoma cell line H441 with wild-type EGFR the antisense miR-21 not only showed the additive effect with AG1478 but also induced apoptosis by itself; These results suggest that aberrantly increased expression of miR-21 which is enhanced further by the activated EGFR signaling pathway plays a significant role in lung carcinogenesis in never-smokers as well as in smokers and is a potential therapeutic target in both EGFR-mutant and wild-type cases;Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR mutated lung cancer via nickel induced microRNA 21 expression;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status ;;progression;drug resistance;staging;drug resistance;tumorigenesis;; lung squamous cell cancer;glioblastoma;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;lung cancer;lung cancer;glioblastoma hsa-miR-29a-5p EGFR -0.113555908626946 0.349622266470788 -1.01672920195188 6.69873186671412e-07 mirMAP -0.458889085026057 2.82252978142708e-08 27477273 Feedback Loop Regulation of SCAP/SREBP 1 by miR 29 Modulates EGFR Signaling Driven Glioblastoma Growth glioblastoma hsa-miR-32-3p EGFR 0.219109440121582 0.207217051619543 -1.01672920195188 6.69873186671412e-07 mirMAP -0.238124126347676 6.9947851263588e-05 NA NA NA hsa-miR-362-5p EGFR 0.717921690799312 1.68392734504524e-05 -1.01672920195188 6.69873186671412e-07 mirMAP -0.247853042376492 3.33470814837779e-05 NA NA NA hsa-miR-3682-3p EGFR 0.805600143458555 8.7874424633131e-06 -1.01672920195188 6.69873186671412e-07 mirMAP -0.12932547288846 0.0249956294814211 NA NA NA hsa-miR-374b-3p EGFR -0.137420230218985 0.305318192448799 -1.01672920195188 6.69873186671412e-07 mirMAP -0.202869152527087 0.00750806275200147 NA NA NA hsa-miR-455-5p EGFR -0.270546034117157 0.0581320703577731 -1.01672920195188 6.69873186671412e-07 miRanda -0.226922724788645 0.00135818927133814 NA NA NA hsa-miR-501-5p EGFR 1.15231083477882 2.98906150004088e-10 -1.01672920195188 6.69873186671412e-07 mirMAP -0.228828416518186 2.10663471825952e-05 NA NA NA hsa-miR-590-5p EGFR -0.103355559980404 0.310027295317092 -1.01672920195188 6.69873186671412e-07 miRanda;mirMAP -0.282971808483034 0.00478577902454241 NA NA NA hsa-miR-618 EGFR 0.13539895350352 0.517145913114103 -1.01672920195188 6.69873186671412e-07 mirMAP -0.104183567265845 0.0380128909987004 NA NA NA hsa-miR-769-5p EGFR 0.219661452072771 0.0333425465750576 -1.01672920195188 6.69873186671412e-07 mirMAP -0.501593765874938 2.76004531715872e-07 NA NA NA hsa-miR-877-5p EGFR 1.35669625926821 1.43008125755656e-11 -1.01672920195188 6.69873186671412e-07 mirMAP -0.247141034396675 1.1588258665615e-06 NA NA NA hsa-miR-15a-5p EGLN1 0.349521211668244 0.000770735998804736 0.0756107370151957 0.405502512247036 MirTarget -0.199232552642064 2.38498365947827e-06 NA NA NA hsa-miR-16-2-3p EGLN1 -0.0336420826463706 0.805158952566973 0.0756107370151957 0.405502512247036 mirMAP -0.154448651591541 1.62881459061765e-06 NA NA NA hsa-miR-16-5p EGLN1 -0.398577235179603 0.000101598495180191 0.0756107370151957 0.405502512247036 MirTarget -0.184906710341522 1.5638193085266e-05 NA NA NA hsa-miR-17-5p EGLN1 0.698861147045461 2.43854106455398e-05 0.0756107370151957 0.405502512247036 TargetScan -0.102696431475396 9.47932370331605e-05 NA NA NA hsa-miR-186-5p EGLN1 -0.060103391373687 0.535288717081205 0.0756107370151957 0.405502512247036 mirMAP -0.139054765598172 0.00251459263459613 NA NA NA hsa-miR-1976 EGLN1 -0.431472440543166 0.00324575010550946 0.0756107370151957 0.405502512247036 MirTarget -0.117052103230428 9.15621715743439e-05 NA NA NA hsa-miR-590-3p EGLN1 -0.473167498047808 1.76652934637227e-05 0.0756107370151957 0.405502512247036 miRanda;mirMAP -0.116534466317335 0.0035050444625743 NA NA NA hsa-miR-590-5p EGLN1 -0.103355559980404 0.310027295317092 0.0756107370151957 0.405502512247036 miRanda -0.196266966880368 6.27078388640176e-06 NA NA NA hsa-miR-10b-3p ETS1 2.77189254628215 2.26687582932628e-20 -0.888146453921594 7.30600721510989e-10 mirMAP -0.105519293114893 1.58415058877614e-05 NA NA NA hsa-miR-1180-3p ETS1 1.13535217859875 1.17286173228245e-09 -0.888146453921594 7.30600721510989e-10 MirTarget -0.293265031973102 8.05459469251981e-16 NA NA NA hsa-miR-130b-5p ETS1 0.170599813152408 0.337611586432771 -0.888146453921594 7.30600721510989e-10 MirTarget -0.117830624648738 0.00350746127707538 NA NA NA hsa-miR-148b-5p ETS1 0.295777213389605 0.0255749859535438 -0.888146453921594 7.30600721510989e-10 MirTarget -0.14433424168597 0.00795478698949807 NA NA NA hsa-miR-16-1-3p ETS1 0.392360028038559 0.00112481918108198 -0.888146453921594 7.30600721510989e-10 MirTarget -0.14163124063757 0.0176332220270728 NA NA NA hsa-miR-186-5p ETS1 -0.060103391373687 0.535288717081205 -0.888146453921594 7.30600721510989e-10 mirMAP -0.15329403808341 0.0411464992332332 NA NA NA hsa-miR-193a-3p ETS1 -0.117675551589896 0.309391958208578 -0.888146453921594 7.30600721510989e-10 miRanda;miRNATAP -0.206450097116881 0.000921512501112203 NA NA NA hsa-miR-221-3p ETS1 1.12244756358612 3.48370308238012e-13 -0.888146453921594 7.30600721510989e-10 miRTarBase;miRNATAP -0.163620716574743 0.000282330030340872 21711453 To close the loop we demonstrate ETS-1 as a direct target of miR-222 but not miR-221 showing the novel option of their uncoupled functions melanoma hsa-miR-222-3p ETS1 1.08574156066353 1.76407192348131e-11 -0.888146453921594 7.30600721510989e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.114027687122358 0.00860678284178252 21711453 Constitutive activation of the ETS 1 miR 222 circuitry in metastatic melanoma; We demonstrate that the proto-oncogene ETS-1 involved in the pathogenesis of cancers of different origin is a transcriptional regulator of miR-222 by direct binding to its promoter region; Differently from 293FT cells or early stage melanomas where unphosphorylated ETS-1 represses miR-222 transcription in metastatic melanoma the constitutively Thr-38 phosphorylated fraction of ETS-1 induces miR-222; To close the loop we demonstrate ETS-1 as a direct target of miR-222 but not miR-221 showing the novel option of their uncoupled functions staging melanoma hsa-miR-30d-3p ETS1 -0.120562286038759 0.329549534782125 -0.888146453921594 7.30600721510989e-10 mirMAP -0.123711965263521 0.0344163749253352 NA NA NA hsa-miR-362-3p ETS1 0.814007335895913 2.00108618836568e-09 -0.888146453921594 7.30600721510989e-10 miRanda -0.181573270953595 0.000463194817826299 NA NA NA hsa-miR-365a-3p ETS1 0.164591326307714 0.15325084963213 -0.888146453921594 7.30600721510989e-10 MirTarget -0.267990900609134 1.68132949421869e-05 NA NA NA hsa-miR-421 ETS1 0.941856334694081 6.12167297054077e-08 -0.888146453921594 7.30600721510989e-10 miRanda;mirMAP;miRNATAP -0.204100092132273 4.23645182774089e-07 NA NA NA hsa-miR-455-5p ETS1 -0.270546034117157 0.0581320703577731 -0.888146453921594 7.30600721510989e-10 MirTarget;PITA;miRanda;miRNATAP -0.234313961624189 2.69025876931775e-06 NA NA NA hsa-miR-501-5p ETS1 1.15231083477882 2.98906150004088e-10 -0.888146453921594 7.30600721510989e-10 mirMAP -0.10302951257088 0.00734880104767461 NA NA NA hsa-miR-502-5p ETS1 0.892373335336396 1.7122694518919e-06 -0.888146453921594 7.30600721510989e-10 miRNATAP -0.110285767636931 0.00447567568456658 NA NA NA hsa-miR-532-3p ETS1 0.297601533259956 0.014632205884815 -0.888146453921594 7.30600721510989e-10 MirTarget;PITA;miRNATAP -0.287398076993315 8.8786296594351e-07 NA NA NA hsa-miR-590-5p ETS1 -0.103355559980404 0.310027295317092 -0.888146453921594 7.30600721510989e-10 miRanda -0.359124442253989 3.41545113201445e-07 NA NA NA hsa-miR-660-5p ETS1 0.989755850734071 5.69930767464937e-14 -0.888146453921594 7.30600721510989e-10 MirTarget -0.254076650434129 1.2827423637958e-06 NA NA NA hsa-miR-769-5p ETS1 0.219661452072771 0.0333425465750576 -0.888146453921594 7.30600721510989e-10 miRNATAP -0.213050530713541 0.00233319206600267 NA NA NA hsa-miR-940 ETS1 0.453020092551484 0.0177054921651997 -0.888146453921594 7.30600721510989e-10 MirTarget;miRNATAP -0.152992991185501 6.8236118996806e-05 NA NA NA hsa-miR-103a-3p FGF2 0.765568928457606 3.05497476509383e-12 -1.08625533231299 0.000324809942833161 miRNAWalker2_validate;MirTarget;miRNATAP -0.333696884446656 0.0118326533065286 NA NA NA hsa-miR-15b-3p FGF2 0.238105490431193 0.0997721500357416 -1.08625533231299 0.000324809942833161 mirMAP -0.499213807743807 9.41955107470528e-07 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-5p FGF2 0.230566941413564 0.0824765001857479 -1.08625533231299 0.000324809942833161 MirTarget;miRNATAP -0.306809751282981 0.00622064491645458 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-16-2-3p FGF2 -0.0336420826463706 0.805158952566973 -1.08625533231299 0.000324809942833161 mirMAP -0.422467965590387 0.000103679884556575 NA NA NA hsa-miR-181a-5p FGF2 0.250113435902954 0.0551933249319293 -1.08625533231299 0.000324809942833161 mirMAP -0.406045352280239 0.000358497143826792 NA NA NA hsa-miR-181b-5p FGF2 0.488256489401108 0.00105321129419701 -1.08625533231299 0.000324809942833161 mirMAP -0.523910277677257 9.1015168945214e-08 NA NA NA hsa-miR-19b-1-5p FGF2 -0.277804782598538 0.0783067794722916 -1.08625533231299 0.000324809942833161 mirMAP -0.529151998934904 1.16181517704286e-08 NA NA NA hsa-miR-200b-3p FGF2 -1.29335788099229 0.000268623234305059 -1.08625533231299 0.000324809942833161 mirMAP -0.168899172593471 4.11351056555817e-05 NA NA NA hsa-miR-200c-3p FGF2 -0.104675615392797 0.716957669484981 -1.08625533231299 0.000324809942833161 mirMAP -0.156084549253076 0.00233819769179705 NA NA NA hsa-miR-20a-3p FGF2 -0.318177901603857 0.0467885456739855 -1.08625533231299 0.000324809942833161 mirMAP -0.492065243098747 8.09310422865092e-08 NA NA NA hsa-miR-21-3p FGF2 -0.484219378716006 0.00299941098417659 -1.08625533231299 0.000324809942833161 mirMAP -0.240197113439825 0.00811114129230132 NA NA NA hsa-miR-21-5p FGF2 1.51084295924322 1.54551622173259e-34 -1.08625533231299 0.000324809942833161 mirMAP -0.443381765546842 5.91228766084995e-05 NA NA NA hsa-miR-215-5p FGF2 -0.981544773246509 3.14452272477156e-05 -1.08625533231299 0.000324809942833161 miRNAWalker2_validate -0.153448795618752 0.0138240489461602 NA NA NA hsa-miR-217 FGF2 1.05797297540381 0.0401026692682422 -1.08625533231299 0.000324809942833161 miRanda -0.127490520581258 7.38698652450206e-06 NA NA NA hsa-miR-222-3p FGF2 1.08574156066353 1.76407192348131e-11 -1.08625533231299 0.000324809942833161 mirMAP -0.206975558906397 0.0212088626227192 NA NA NA hsa-miR-330-3p FGF2 -0.332364259424489 0.0316051913061459 -1.08625533231299 0.000324809942833161 mirMAP -0.238733647850468 0.0151404054794887 NA NA NA hsa-miR-335-3p FGF2 -0.275199247483111 0.106629165248783 -1.08625533231299 0.000324809942833161 mirMAP -0.405107692314837 2.67658505476485e-06 NA NA NA hsa-miR-338-5p FGF2 -0.221898432884726 0.252390988727086 -1.08625533231299 0.000324809942833161 mirMAP -0.277477917380717 0.000298421894309671 NA NA NA hsa-miR-429 FGF2 -1.40262535827285 6.93982219171912e-05 -1.08625533231299 0.000324809942833161 mirMAP -0.192074793475977 4.45604159403906e-06 NA NA NA hsa-miR-452-5p FGF2 1.91544498799508 1.81995982853313e-14 -1.08625533231299 0.000324809942833161 mirMAP -0.254292038125162 7.84361502048493e-06 NA NA NA hsa-miR-589-3p FGF2 1.16846704028095 5.88525574424201e-11 -1.08625533231299 0.000324809942833161 mirMAP -0.346323302130209 2.68946343007257e-05 NA NA NA hsa-miR-590-5p FGF2 -0.103355559980404 0.310027295317092 -1.08625533231299 0.000324809942833161 miRanda;mirMAP -0.300649640419796 0.0415702477858108 NA NA NA hsa-miR-92a-3p FGF2 0.212391012518195 0.134292268089997 -1.08625533231299 0.000324809942833161 miRNAWalker2_validate -0.635934402042256 6.72536311164143e-10 NA NA NA hsa-miR-93-3p FGF2 0.402821129107592 0.00131324842735588 -1.08625533231299 0.000324809942833161 mirMAP -0.437085459323848 0.000209985380897254 NA NA NA hsa-miR-100-5p FGFR3 -0.783576782126614 0.000219958772262677 0.189024805500786 0.340204942325551 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.269596147351536 1.27205852375091e-09 25344675;26604796;26018508;23778527 MicroRNA 100 regulates pancreatic cancer cells growth and sensitivity to chemotherapy through targeting FGFR3; The predicted target of miR-100 fibroblast growth factor receptor 3 FGFR3 was downregulated by siRNA to examine its effect on pancreatic cancer cells; Luciferase essay showed FGFR3 was direct target of miR-100; FGFR3 was significantly downregulated by overexpressing miR-100 in pancreatic cancer cells and knocking down FGFR3 by siRNA exerted similar effect as miR-100; Our study demonstrated that miR-100 played an important role in pancreatic cancer development possibly through targeting FGFR3;Overexpression of miR 100 inhibits cell proliferation migration and chemosensitivity in human glioblastoma through FGFR3; Expression of fibroblast growth factor receptor 3 FGFR3 the bioinformatically predicted target of miR-100 was examined by Western blot in glioblastoma; FGFR3 was directly regulated by miR-100 in glioblastoma; Ectopically overexpressing FGFR3 was able to ameliorate the anticancer effects of upregulation of miR-100 on glioblastoma growth migration and chemosensitivity; Overexpressing miR-100 had anticancer effects on glioblastoma likely through regulation of FGFR3;Overexpression of miR 100 inhibits growth of osteosarcoma through FGFR3; Here we reported significantly higher levels of fibroblast growth factor receptor 3 FGFR3 and significantly lower levels of miR-100 in the OS specimen compared to those in the paired normal bone tissues; Bioinformatics analysis and luciferase reporter assay suggest that miR-100 binds to the 3'UTR of FGFR3 mRNA to prevent its translation; Taken together our data demonstrate that miR-100 may inhibit the growth of OS through FGFR3;Hypoxia regulates FGFR3 expression via HIF 1α and miR 100 and contributes to cell survival in non muscle invasive bladder cancer; We have previously investigated the role of microRNAs in bladder cancer and have shown that FGFR3 is a target of miR-100; In this study we investigated the effects of hypoxia on miR-100 and FGFR3 expression and the link between miR-100 and FGFR3 in hypoxia; Bladder cancer cell lines were exposed to normoxic or hypoxic conditions and examined for the expression of FGFR3 by quantitative PCR qPCR and western blotting and miR-100 by qPCR; The effect of FGFR3 and miR-100 on cell viability in two-dimensional 2-D and three-dimensional 3-D was examined by transfecting siRNA or mimic-100 respectively; Increased FGFR3 was also in part dependent on miR-100 levels which decreased in hypoxia; Hypoxia in part via suppression of miR-100 induces FGFR3 expression in bladder cancer both of which have an important role in maintaining cell viability under conditions of stress ;;;poor survival pancreatic cancer;glioblastoma;sarcoma;bladder cancer hsa-miR-590-3p FGFR3 -0.473167498047808 1.76652934637227e-05 0.189024805500786 0.340204942325551 miRanda -0.178376084373127 0.0416868756939642 NA NA NA hsa-miR-99a-5p FGFR3 -1.51481656384839 3.93230057259529e-14 0.189024805500786 0.340204942325551 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.302096873169716 3.68370160731018e-11 23409016;24456664;23298836 Photofrin based photodynamic therapy and miR 99a transfection inhibited FGFR3 and PI3K/Akt signaling mechanisms to control growth of human glioblastoma In vitro and in vivo; Further photofrin based PDT followed by miR-99a transfection dramatically increased miR-99a expression and also increased apoptosis in glioblastoma cell cultures and drastically reduced tumor growth in athymic nude mice due to down regulation of fibroblast growth factor receptor 3 FGFR3 and PI3K/Akt signaling mechanisms leading to inhibition of cell proliferation and induction of molecular mechanisms of apoptosis;miR 99a promotes proliferation targeting FGFR3 in human epithelial ovarian cancer cells; Importantly fibroblast growth factor receptor 3 FGFR3 predicted to be one target gene of miR-99a using computational algorithms was higher in expression in EOC cells; Subsequently FGFR3 was proved to be direct target of miR-99a by dual luciferase assay; Furthermore overexpression of miR-99a dramatically suppressed expression level of FGFR3 at both mRNA and protein levels proving FGFR3 to be inversely correlated with miR-99a; Finally overexpression of miR-99a could significantly inhibit EOC cell proliferation in vitro by decreasing the expression of FGFR3 which also reduced the EOC cell growth after siRNA knockdown; Conclusively miR-99a expression was remarkably downregulated in serums tissues and cell and suppresses EOC cell proliferation by targeting FGFR3 suggesting miR-99a as a prospective prognosis marker and potential tumor suppressor for EOC therapeutics;The tumorigenic FGFR3 TACC3 gene fusion escapes miR 99a regulation in glioblastoma; The fusion caused by tandem duplication on 4p16.3 led to the loss of the 3'-UTR of FGFR3 blocking gene regulation of miR-99a and enhancing expression of the fusion gene ;worse prognosis; glioblastoma;ovarian cancer;glioblastoma hsa-miR-101-3p FZD6 -1.48176407980989 3.98950707385704e-31 0.589500198001939 0.00374127878510925 miRNAWalker2_validate;MirTarget -0.312670748498135 1.38907823475328e-05 NA NA NA hsa-miR-139-5p FZD6 -2.11299531796557 8.16407076415038e-28 0.589500198001939 0.00374127878510925 miRanda -0.171390807110702 0.00034847427750895 NA NA NA hsa-miR-194-5p FZD6 -0.292530160992458 0.0996141384876663 0.589500198001939 0.00374127878510925 MirTarget -0.265420163621645 1.75074747054336e-06 NA NA NA hsa-miR-455-5p FZD6 -0.270546034117157 0.0581320703577731 0.589500198001939 0.00374127878510925 miRanda -0.230555626016252 0.000940179578305785 NA NA NA hsa-miR-107 HIF1A 0.241662461253461 0.0170788899505389 -0.366176828296648 0.0111002186054683 miRNAWalker2_validate;miRTarBase;miRanda -0.436710343430652 2.03313957855482e-10 NA NA NA hsa-miR-17-5p HIF1A 0.698861147045461 2.43854106455398e-05 -0.366176828296648 0.0111002186054683 miRTarBase;MirTarget;TargetScan -0.207486649592262 6.73419034465465e-07 NA NA NA hsa-miR-20a-5p HIF1A 0.854326758727003 5.04256669663285e-07 -0.366176828296648 0.0111002186054683 miRNAWalker2_validate;miRTarBase;MirTarget -0.245558453559623 9.83134714947215e-10 22901144 Correlation analysis showed that the key miRNAs miR-20a and miR-20b negatively correlated with the target proteins VEGF-A and HIF-1alpha breast cancer hsa-miR-33a-5p HIF1A -0.767474460958522 1.09948756118522e-05 -0.366176828296648 0.0111002186054683 miRNAWalker2_validate -0.107227499920455 0.00730487730363833 NA NA NA hsa-miR-616-5p HIF1A 0.150569904638223 0.402836556304524 -0.366176828296648 0.0111002186054683 MirTarget -0.124976106072862 0.00144238259369066 NA NA NA hsa-miR-660-5p HIF1A 0.989755850734071 5.69930767464937e-14 -0.366176828296648 0.0111002186054683 MirTarget -0.249870386379118 1.21860559731773e-06 NA NA NA hsa-miR-93-5p HIF1A 1.39518349670953 2.84167728726712e-25 -0.366176828296648 0.0111002186054683 MirTarget -0.173769064325932 0.000425501783480084 NA NA NA hsa-miR-125a-5p IKBKG -0.91219969372382 1.38256246489548e-07 0.660366120199928 1.71867865546723e-06 miRanda -0.219029418282295 9.51829321882113e-09 NA NA NA hsa-miR-143-3p IKBKG -0.584123869220553 0.000907217687997436 0.660366120199928 1.71867865546723e-06 mirMAP -0.169551761758995 9.09905571393035e-06 NA NA NA hsa-miR-200a-3p IKBKG -1.49949292595657 3.18410995256133e-05 0.660366120199928 1.71867865546723e-06 mirMAP -0.100439586597671 5.62621816828972e-08 NA NA NA hsa-miR-24-3p IKBKG -0.257825594021966 0.00689742600215061 0.660366120199928 1.71867865546723e-06 mirMAP -0.287544034512102 5.8686253015948e-05 NA NA NA hsa-miR-3065-3p IKBKG -1.04244468016704 4.60743424372577e-05 0.660366120199928 1.71867865546723e-06 mirMAP -0.115721435331344 9.61381024877344e-06 NA NA NA hsa-miR-320a IKBKG 0.325285936317332 0.0221415065838824 0.660366120199928 1.71867865546723e-06 mirMAP -0.182311319218153 0.000133876392194021 NA NA NA hsa-miR-320b IKBKG 0.0919652022571331 0.607981643616945 0.660366120199928 1.71867865546723e-06 mirMAP -0.132285547849802 0.000522961413620221 NA NA NA hsa-miR-330-3p IKBKG -0.332364259424489 0.0316051913061459 0.660366120199928 1.71867865546723e-06 mirMAP -0.111083773167518 0.0137533606676294 NA NA NA hsa-miR-335-5p IKBKG -1.61347836513558 1.62757083289711e-19 0.660366120199928 1.71867865546723e-06 mirMAP -0.273399124951207 9.32430291537739e-15 NA NA NA hsa-miR-338-5p IKBKG -0.221898432884726 0.252390988727086 0.660366120199928 1.71867865546723e-06 mirMAP -0.206675822417303 2.68501101428877e-09 NA NA NA hsa-miR-376c-3p IKBKG -1.78544013329054 1.82972185127691e-10 0.660366120199928 1.71867865546723e-06 mirMAP -0.14083607992738 1.85430800686914e-09 NA NA NA hsa-miR-542-3p IKBKG -1.31351015149842 3.64642423288576e-22 0.660366120199928 1.71867865546723e-06 mirMAP -0.292142688932618 4.40119371820177e-10 NA NA NA hsa-miR-125b-2-3p ITGA6 -1.6609436107315 5.71534047969855e-18 1.33116884445099 5.73067512733422e-21 mirMAP -0.177305489469283 7.67219603417741e-07 NA NA NA hsa-miR-199a-3p ITGA6 -2.3279328685682 1.04468066198593e-17 1.33116884445099 5.73067512733422e-21 PITA -0.166829140227348 2.64008048809431e-11 NA NA NA hsa-miR-199b-3p ITGA6 -2.33226605401203 9.51887435273314e-18 1.33116884445099 5.73067512733422e-21 PITA -0.166957893782338 2.45787733398932e-11 NA NA NA hsa-miR-335-3p ITGA6 -0.275199247483111 0.106629165248783 1.33116884445099 5.73067512733422e-21 mirMAP -0.138340364120149 0.00114101751107001 NA NA NA hsa-miR-342-3p ITGA6 -0.319677314984262 0.0449813878317147 1.33116884445099 5.73067512733422e-21 miRanda -0.124580956342346 0.00625142354926915 NA NA NA hsa-miR-192-5p ITGAV -0.50188499512787 0.00344713341196035 0.42083458357731 0.00760476174317356 miRNAWalker2_validate;MirTarget -0.344370980466671 1.89013250176103e-15 NA NA NA hsa-miR-32-5p ITGAV 0.0806126674664016 0.548984992497432 0.42083458357731 0.00760476174317356 miRNAWalker2_validate;MirTarget;miRNATAP -0.274975166498264 1.42681810268729e-06 NA NA NA hsa-miR-328-3p ITGAV -0.547466865311955 6.8359574451572e-06 0.42083458357731 0.00760476174317356 MirTarget -0.132063082039302 0.0363785445433637 NA NA NA hsa-miR-33a-3p ITGAV -0.683025859637737 1.01106097756419e-05 0.42083458357731 0.00760476174317356 MirTarget -0.155984185219414 0.00158654023964867 NA NA NA hsa-miR-374a-5p ITGAV 0.016390363747302 0.869784217438621 0.42083458357731 0.00760476174317356 mirMAP -0.174068026833335 0.0257907946480254 NA NA NA hsa-miR-92a-3p ITGAV 0.212391012518195 0.134292268089997 0.42083458357731 0.00760476174317356 MirTarget;miRNATAP -0.257814482101981 1.80305424957411e-06 NA NA NA hsa-miR-106b-5p JAK1 0.648901865047099 8.24870862047885e-09 -0.491502741052473 2.21647945606145e-08 miRNAWalker2_validate;MirTarget -0.274241391983959 1.386588824919e-13 NA NA NA hsa-miR-128-3p JAK1 0.289735108018712 0.0114419474207889 -0.491502741052473 2.21647945606145e-08 miRNAWalker2_validate -0.109369528499963 0.00404231658945177 NA NA NA hsa-miR-17-5p JAK1 0.698861147045461 2.43854106455398e-05 -0.491502741052473 2.21647945606145e-08 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.268052325740918 3.25515675433912e-28 NA NA NA hsa-miR-20a-5p JAK1 0.854326758727003 5.04256669663285e-07 -0.491502741052473 2.21647945606145e-08 MirTarget -0.23386116447226 1.56734608485868e-22 NA NA NA hsa-miR-30d-5p JAK1 0.718283816348023 2.47422074061352e-08 -0.491502741052473 2.21647945606145e-08 MirTarget -0.145093822003298 1.12748945024431e-05 NA NA NA hsa-miR-455-5p JAK1 -0.270546034117157 0.0581320703577731 -0.491502741052473 2.21647945606145e-08 miRanda -0.167977882302863 2.26382130466332e-08 NA NA NA hsa-miR-93-5p JAK1 1.39518349670953 2.84167728726712e-25 -0.491502741052473 2.21647945606145e-08 MirTarget;miRNATAP -0.238027881672368 6.65509610107069e-16 NA NA NA hsa-miR-140-3p KRAS 0.546012239205329 4.84578261098777e-11 -0.338081744192936 0.00043842779003105 MirTarget -0.247198429138234 9.22756583574077e-06 NA NA NA hsa-miR-16-1-3p KRAS 0.392360028038559 0.00112481918108198 -0.338081744192936 0.00043842779003105 mirMAP -0.114704330599848 0.00330327136149234 NA NA NA hsa-miR-193a-3p KRAS -0.117675551589896 0.309391958208578 -0.338081744192936 0.00043842779003105 MirTarget;miRanda;miRNATAP -0.128182356310374 0.00170700856743147 NA NA NA hsa-miR-193b-3p KRAS -0.166697207281748 0.272022543069482 -0.338081744192936 0.00043842779003105 miRNAWalker2_validate;MirTarget;miRNATAP -0.120510531869791 9.78248377856047e-05 25905463 Deregulation of the MiR 193b KRAS Axis Contributes to Impaired Cell Growth in Pancreatic Cancer; Mechanistically KRAS was verified as a direct effector of miR-193b through which the AKT and ERK pathways were modulated and cell growth of PDAC cells was suppressed; Taken together our findings indicate that miR-193b-mediated deregulation of the KRAS axis is involved in pancreatic carcinogenesis and suggest that miR-193b could be a potentially effective target for PDAC therapy tumorigenesis pancreatic cancer hsa-miR-30d-3p KRAS -0.120562286038759 0.329549534782125 -0.338081744192936 0.00043842779003105 MirTarget;miRNATAP -0.132565215112955 0.000512493362671086 NA NA NA hsa-miR-532-5p KRAS 1.02729546510601 9.93318913966636e-16 -0.338081744192936 0.00043842779003105 MirTarget;PITA;miRNATAP -0.137739612561571 0.000100604507963156 NA NA NA hsa-miR-146b-5p LAMA2 0.419719546795449 0.0457375823220155 -2.25093797189139 1.13318229560757e-08 miRanda -0.200973106106506 0.0311052794529279 NA NA NA hsa-miR-324-5p LAMB3 0.372933276649782 0.00592280066108904 0.205469745450436 0.402537496980396 miRanda -0.210911510332022 0.017049035981626 NA NA NA hsa-miR-140-3p MAP2K1 0.546012239205329 4.84578261098777e-11 -1.22814648702538 4.27448032410174e-41 PITA -0.381987666476187 3.26697824928144e-11 NA NA NA hsa-miR-34a-5p MAP2K1 1.03694231790865 5.74864019540594e-14 -1.22814648702538 4.27448032410174e-41 miRNAWalker2_validate;miRTarBase -0.229470281840033 1.32655977381036e-11 NA NA NA hsa-miR-361-5p MAP2K1 0.233202959858696 0.00961572812255327 -1.22814648702538 4.27448032410174e-41 miRanda -0.180575442044722 0.00107072926955335 NA NA NA hsa-miR-501-5p MAP2K1 1.15231083477882 2.98906150004088e-10 -1.22814648702538 4.27448032410174e-41 PITA -0.144135390798289 3.07397683406163e-08 NA NA NA hsa-miR-19b-3p MECOM 0.601279551368824 0.00016685097085251 0.959512171082984 9.09579943703071e-08 miRNATAP -0.129331453668629 0.0196315279663432 NA NA NA hsa-miR-22-3p MECOM -0.628561621952407 1.33755558015523e-09 0.959512171082984 9.09579943703071e-08 miRNATAP -0.180980334358813 0.0330564397272789 NA NA NA hsa-miR-361-5p MECOM 0.233202959858696 0.00961572812255327 0.959512171082984 9.09579943703071e-08 miRanda -0.278956690972315 0.00517024358546618 NA NA NA hsa-miR-374a-5p MECOM 0.016390363747302 0.869784217438621 0.959512171082984 9.09579943703071e-08 mirMAP -0.37178528301155 3.21945126868006e-05 NA NA NA hsa-miR-374b-5p MECOM -0.307538028546424 0.0030079617545972 0.959512171082984 9.09579943703071e-08 mirMAP -0.372614882805562 1.35293538346028e-05 NA NA NA hsa-miR-455-5p MECOM -0.270546034117157 0.0581320703577731 0.959512171082984 9.09579943703071e-08 miRanda -0.34626889403173 1.80675931190258e-08 NA NA NA hsa-miR-125a-3p MSH3 -0.841683158494395 3.51202687176603e-05 -0.161343786741238 0.0575589260293181 miRanda -0.118807690934592 4.43923977822084e-09 NA NA NA hsa-miR-92b-3p MSH3 0.220374930803974 0.296187538889092 -0.161343786741238 0.0575589260293181 miRNAWalker2_validate -0.140228793554641 1.74398308418954e-13 NA NA NA hsa-miR-15a-5p NFKB1 0.349521211668244 0.000770735998804736 -0.538916955748098 1.42993670239858e-08 miRNAWalker2_validate -0.153048958379264 0.000730484492411411 NA NA NA hsa-miR-548j-5p NFKB1 0.251239647701947 0.171361975450222 -0.538916955748098 1.42993670239858e-08 MirTarget -0.106885227436574 4.88574003092244e-05 NA NA NA hsa-miR-92a-3p NFKB1 0.212391012518195 0.134292268089997 -0.538916955748098 1.42993670239858e-08 miRNAWalker2_validate -0.147016189975437 8.47280185007855e-06 NA NA NA hsa-miR-146b-3p NOS2 -1.20799545896423 2.19859530890688e-09 1.34188199912716 2.31721049529452e-07 MirTarget;miRNATAP -0.178469037014934 0.00416937467719295 NA NA NA hsa-let-7b-5p NRAS -0.959013717270803 5.95127281258643e-11 0.301156333096785 0.000285671613438218 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.105903409272711 8.57245668973746e-05 20607356 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells glioblastoma hsa-miR-126-5p NRAS -0.428561469405594 6.6035585844222e-05 0.301156333096785 0.000285671613438218 mirMAP -0.145651073417946 0.000106824688349933 NA NA NA hsa-miR-145-5p NRAS -1.4795885711477 6.47308361543414e-16 0.301156333096785 0.000285671613438218 miRNAWalker2_validate;MirTarget;miRNATAP -0.120395847533196 1.03194548202048e-08 26973415 miR-145 expression was significantly downregulated in colon cancer tissues with its expression in normal colonic tissues being 4-5-fold higher two sample t test P < 0.05 whereas N-ras expression showed the opposite trend colon cancer hsa-miR-22-3p NRAS -0.628561621952407 1.33755558015523e-09 0.301156333096785 0.000285671613438218 MirTarget;miRNATAP -0.287363482934569 1.84836871548684e-14 NA NA NA hsa-miR-29a-3p NRAS -0.856762511178136 4.65330336378459e-12 0.301156333096785 0.000285671613438218 miRNATAP -0.111209986118726 0.000497462508043792 NA NA NA hsa-miR-29b-3p NRAS -0.348885528720517 0.0121441341291285 0.301156333096785 0.000285671613438218 miRNATAP -0.163441778679427 1.31476396622707e-08 NA NA NA hsa-miR-29c-3p NRAS -1.4367517608807 1.61767774905781e-21 0.301156333096785 0.000285671613438218 miRNATAP -0.195416271463693 3.04370398757482e-15 NA NA NA hsa-miR-664a-3p NRAS 0.485807855553031 0.000732553249798598 0.301156333096785 0.000285671613438218 MirTarget -0.123577371649095 9.58480979576424e-06 NA NA NA hsa-miR-342-3p PIAS1 -0.319677314984262 0.0449813878317147 0.14110668905439 0.111009722342972 miRanda -0.127221288569843 1.97391983714843e-06 NA NA NA hsa-miR-103a-3p PIK3R1 0.765568928457606 3.05497476509383e-12 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.187692597616198 0.00702590214654354 NA NA NA hsa-miR-106b-5p PIK3R1 0.648901865047099 8.24870862047885e-09 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.369244291882445 4.59379549667075e-08 NA NA NA hsa-miR-1301-3p PIK3R1 1.12156714169769 4.22404125385203e-10 -0.891953424131491 1.38465728969785e-08 MirTarget -0.26088137729301 3.97606431390706e-10 NA NA NA hsa-miR-132-3p PIK3R1 0.323796239695997 0.00272093067935091 -0.891953424131491 1.38465728969785e-08 MirTarget -0.517351445825174 2.17222827385203e-13 NA NA NA hsa-miR-15b-5p PIK3R1 0.230566941413564 0.0824765001857479 -0.891953424131491 1.38465728969785e-08 MirTarget -0.132325827339178 0.0249531550846148 NA NA NA hsa-miR-17-5p PIK3R1 0.698861147045461 2.43854106455398e-05 -0.891953424131491 1.38465728969785e-08 MirTarget;TargetScan;miRNATAP -0.213954808771155 3.8587060059728e-06 NA NA NA hsa-miR-185-5p PIK3R1 0.480006257154717 6.35758891340152e-07 -0.891953424131491 1.38465728969785e-08 miRNATAP -0.255181123475439 0.00153792085091181 NA NA NA hsa-miR-188-5p PIK3R1 1.11646899351496 9.56686958408598e-11 -0.891953424131491 1.38465728969785e-08 MirTarget -0.178093775716465 5.33015486626271e-05 NA NA NA hsa-miR-200c-3p PIK3R1 -0.104675615392797 0.716957669484981 -0.891953424131491 1.38465728969785e-08 mirMAP -0.105107802462856 9.06963579759966e-05 NA NA NA hsa-miR-20a-5p PIK3R1 0.854326758727003 5.04256669663285e-07 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.137431012149634 0.00237710222818209 NA NA NA hsa-miR-21-5p PIK3R1 1.51084295924322 1.54551622173259e-34 -0.891953424131491 1.38465728969785e-08 miRNAWalker2_validate;MirTarget;miRNATAP -0.626634426633867 6.01244523464713e-31 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-212-3p PIK3R1 -0.288359631986802 0.100392159518997 -0.891953424131491 1.38465728969785e-08 MirTarget -0.258090192317157 3.95293509038579e-09 NA NA NA hsa-miR-221-3p PIK3R1 1.12244756358612 3.48370308238012e-13 -0.891953424131491 1.38465728969785e-08 MirTarget -0.217914846238424 7.87055620620445e-06 NA NA NA hsa-miR-222-3p PIK3R1 1.08574156066353 1.76407192348131e-11 -0.891953424131491 1.38465728969785e-08 MirTarget -0.256619417997498 3.41697791848384e-08 NA NA NA hsa-miR-324-3p PIK3R1 0.260348469146494 0.0506141704203545 -0.891953424131491 1.38465728969785e-08 MirTarget;PITA;miRNATAP -0.281792149179102 1.26555231509763e-06 NA NA NA hsa-miR-330-3p PIK3R1 -0.332364259424489 0.0316051913061459 -0.891953424131491 1.38465728969785e-08 MirTarget;PITA;miRNATAP -0.212492102885122 3.44445995574159e-05 NA NA NA hsa-miR-338-5p PIK3R1 -0.221898432884726 0.252390988727086 -0.891953424131491 1.38465728969785e-08 PITA -0.143685656767977 0.000366657184559181 NA NA NA hsa-miR-409-3p PIK3R1 -0.499649792570937 0.0526751181520117 -0.891953424131491 1.38465728969785e-08 mirMAP -0.110448878014769 0.000237041711390918 NA NA NA hsa-miR-493-5p PIK3R1 -0.198321662045422 0.502870277689198 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.103739426368655 7.76271840065789e-05 NA NA NA hsa-miR-582-5p PIK3R1 -0.680189083316822 0.00103755166534107 -0.891953424131491 1.38465728969785e-08 mirMAP -0.138461163257927 0.00021364752999012 NA NA NA hsa-miR-589-3p PIK3R1 1.16846704028095 5.88525574424201e-11 -0.891953424131491 1.38465728969785e-08 mirMAP -0.21021578613876 1.13421126603334e-06 NA NA NA hsa-miR-590-5p PIK3R1 -0.103355559980404 0.310027295317092 -0.891953424131491 1.38465728969785e-08 MirTarget;PITA;miRanda;miRNATAP -0.299776876388425 9.9413226282672e-05 NA NA NA hsa-miR-629-3p PIK3R1 -0.315338123880353 0.119091248294946 -0.891953424131491 1.38465728969785e-08 MirTarget -0.182940394280375 3.19896248240917e-06 NA NA NA hsa-miR-93-5p PIK3R1 1.39518349670953 2.84167728726712e-25 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.319783065556515 2.89613370212511e-09 NA NA NA hsa-let-7b-5p PLK1 -0.959013717270803 5.95127281258643e-11 3.58200503456178 1.01936996392609e-41 miRNAWalker2_validate -0.508374715415364 7.45675351637923e-08 NA NA NA hsa-miR-100-5p PLK1 -0.783576782126614 0.000219958772262677 3.58200503456178 1.01936996392609e-41 miRNAWalker2_validate;miRTarBase -0.609428308452059 1.35398975085084e-21 23842624;23151088;22246341;25537513;22120675;21636267 In HCC tissues miR-100 expression was inversely correlated with the expression of plk1 protein r = -0.418; P = 0.029; Therefore downregulation of miR-100 was correlated with progressive pathological feature and poor prognosis in HCC patients and miR-100 could function as a tumor suppressor by targeting plk1;MicroRNA 100 is a potential molecular marker of non small cell lung cancer and functions as a tumor suppressor by targeting polo like kinase 1; By using microRNA miR target prediction algorithms we identified miR-100 that might potentially bind the 3'-untranslated region of PLK1 transcripts; The purpose of this study was to investigate the roles of miR-100 and its association with PLK1 in NSCLC development; Finally the effects of miR-100 expression on growth apoptosis and cell cycle of NSCLC cells by posttranscriptionally regulating PLK1 expression were determined; Meanwhile miR-100 mimics could significantly inhibit PLK1 mRNA and protein expression and reduce the luciferase activity of a PLK1 3' untranslated region-based reporter construct in A549 cells; Furthermore small interfering RNA siRNA-mediated PLK1 downregulation could mimic the effects of miR-100 mimics while PLK1 overexpression could partially rescue the phenotypical changes of NSCLC cells induced by miR-100 mimics; Our findings indicate that low miR-100 may be a poor prognostic factor for NSCLC patients and functions as a tumor suppressor by posttranscriptionally regulating PLK1 expression;Together these results suggest that low miR-100 expression may be an independent poor prognostic factor and miR-100 can function as a tumor suppressor by targeting PLK1 in human EOCs;Here we show that miR-100 inhibits maintenance and expansion of BrCSCs in basal-like cancer through Polo-like kinase1 Plk1 down-regulation;MiR 100 resensitizes docetaxel resistant human lung adenocarcinoma cells SPC A1 to docetaxel by targeting Plk1; Knock-down of Plk1 which was a direct target of miR-100 yielded similar effects as that of ectopic miR-100 expression; The inverse correlation between miR-100 and Plk1 expression was also detected in nude mice SPC-A1/DTX tumor xenografts and clinical lung adenocarcinoma tissues and was proved to be related with the in vivo response to docetaxel; Thus our results suggested that down-regulation of miR-100 could lead to Plk1 over-expression and eventually to docetaxel chemoresistance of human lung adenocarcinoma;Reduced miR 100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein; Through modulating miR-100 expression using miR-100 inhibitor or mimic in vitro cell growth cycle and apoptosis were tested separately by MTT or flow cytometry and meanwhile Polo-like kinase1 PLK1 mRNA and protein expressions were detected by qRT-PCR and immunoblotting; The expression of PLK1 in 125 cervical tissues was also examined by immunohistochemical staining and the correlation between miR-100 and PLK1 expression in the same tissues was analysed; The modulation of miR-100 expression remarkably influenced cell proliferation cycle and apoptosis as well as the level of PLK1 protein but not mRNA in vitro experiments; PLK1 expression was negatively correlated with miR-100 expression in CIN3 and cervical cancer tissues; The reduced miR-100 expression participates in the development of cervical cancer at least partly through loss of inhibition to target gene PLK1 which probably occurs in a relative late phase of carcinogenesis progression;worse prognosis;;;;drug resistance;tumorigenesis liver cancer;lung squamous cell cancer;ovarian cancer;breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-486-5p PLK1 -1.77766890600022 2.9071717929922e-14 3.58200503456178 1.01936996392609e-41 miRanda -0.373122790226321 1.30117161322891e-10 NA NA NA hsa-miR-505-5p PLK1 -0.768282569967001 1.14662846595407e-05 3.58200503456178 1.01936996392609e-41 MirTarget -0.372253566265304 3.64975232604557e-06 NA NA NA hsa-miR-874-3p PLK1 -1.07043026298634 2.09564226589678e-07 3.58200503456178 1.01936996392609e-41 miRNAWalker2_validate -0.236755899629093 0.000522664320516165 NA NA NA hsa-miR-194-5p PRKCB -0.292530160992458 0.0996141384876663 -1.62067363753233 3.76201992658756e-13 miRNATAP -0.310861421038956 6.51903986219064e-07 NA NA NA hsa-miR-23b-3p PRKCB -0.530561587109023 5.43807206823652e-07 -1.62067363753233 3.76201992658756e-13 mirMAP -0.286204121948097 0.00657996649038471 NA NA NA hsa-miR-30d-5p PRKCB 0.718283816348023 2.47422074061352e-08 -1.62067363753233 3.76201992658756e-13 mirMAP -0.362125630736335 2.22146697946039e-05 NA NA NA hsa-miR-340-5p PRKCB -0.00477614531381576 0.968502722773791 -1.62067363753233 3.76201992658756e-13 mirMAP -0.225497683155663 0.0158024495898231 NA NA NA hsa-miR-361-5p PRKCB 0.233202959858696 0.00961572812255327 -1.62067363753233 3.76201992658756e-13 MirTarget;miRanda;miRNATAP -0.424918272347972 0.000711090604030861 NA NA NA hsa-miR-589-3p PRKCB 1.16846704028095 5.88525574424201e-11 -1.62067363753233 3.76201992658756e-13 mirMAP -0.228692909978861 0.000252673885842927 NA NA NA hsa-miR-589-5p PRKCB 1.18593770505086 1.01781241960531e-22 -1.62067363753233 3.76201992658756e-13 mirMAP -0.552773953594914 1.64276000301029e-10 NA NA NA hsa-miR-616-5p PRKCB 0.150569904638223 0.402836556304524 -1.62067363753233 3.76201992658756e-13 mirMAP -0.245704989615516 7.89898683278172e-05 NA NA NA hsa-miR-664a-3p PRKCB 0.485807855553031 0.000732553249798598 -1.62067363753233 3.76201992658756e-13 mirMAP -0.348212449855556 6.35284546408206e-06 NA NA NA hsa-miR-19b-1-5p RALBP1 -0.277804782598538 0.0783067794722916 0.1698024329452 0.0449773531520714 mirMAP -0.107718420080393 3.35490340732313e-05 NA NA NA hsa-miR-146b-5p RARA 0.419719546795449 0.0457375823220155 0.26381183745838 0.00504882339445867 miRanda -0.113842988881595 1.2506902289011e-07 NA NA NA hsa-miR-107 RASSF5 0.241662461253461 0.0170788899505389 -0.908227788377981 1.02390034106228e-08 PITA;miRanda;miRNATAP -0.284285927003021 0.000267803442305389 NA NA NA hsa-miR-421 RASSF5 0.941856334694081 6.12167297054077e-08 -0.908227788377981 1.02390034106228e-08 miRanda -0.134754778124398 0.00257128215281031 NA NA NA hsa-miR-532-5p RASSF5 1.02729546510601 9.93318913966636e-16 -0.908227788377981 1.02390034106228e-08 PITA;miRNATAP -0.217078156594971 0.000253741557038178 NA NA NA hsa-miR-324-5p ROCK1 0.372933276649782 0.00592280066108904 -0.264366618624451 0.00575875249305368 MirTarget;PITA;miRanda -0.132464043588386 0.000113488217308036 NA NA NA hsa-miR-17-5p ROCK2 0.698861147045461 2.43854106455398e-05 -0.343723141838983 0.0978147147700846 TargetScan -0.230848684676223 0.000132737616833034 NA NA NA hsa-miR-181a-5p SMC1A 0.250113435902954 0.0551933249319293 0.0651833474337025 0.510370716253497 mirMAP -0.117542893899761 0.00146146139664929 NA NA NA hsa-miR-181c-5p SMC1A -0.00639394152050299 0.969127212255928 0.0651833474337025 0.510370716253497 mirMAP -0.102787280067137 0.000412049730773496 NA NA NA hsa-miR-193b-3p SMC3 -0.166697207281748 0.272022543069482 0.203644948628179 0.0850721957839735 miRNAWalker2_validate -0.129842117232487 0.000624818169450842 NA NA NA hsa-miR-30b-5p STAG1 -0.54056186708617 2.44688760798341e-05 -0.178198619776885 0.243967920887864 MirTarget -0.20850853639894 0.00030033116479739 NA NA NA hsa-miR-30d-5p STAG1 0.718283816348023 2.47422074061352e-08 -0.178198619776885 0.243967920887864 MirTarget;mirMAP -0.140033891643882 0.0142456491944109 NA NA NA hsa-miR-106b-5p TGFBR2 0.648901865047099 8.24870862047885e-09 -0.522706741081292 3.47206054315007e-05 miRNATAP -0.368277930860382 4.29748674542316e-12 NA NA NA hsa-miR-130b-3p TGFBR2 0.687190305027546 0.000109068658562255 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRNATAP -0.131252693120461 0.000144540113312326 25024357 Follow-up experiments showed two miRNAs miR-9-5p and miR-130b-3p in this module had increased expression while their target gene TGFBR2 had decreased expression in a cohort of human NSCLC lung squamous cell cancer hsa-miR-17-5p TGFBR2 0.698861147045461 2.43854106455398e-05 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.203186001893041 2.94531720390227e-08 25011053;27120811 We demonstrate that miR-17 overexpression interferes with the TGFβ-EMT axis and hinders RCC sphere formation; and validated TGFBR2 as a direct and biologically relevant target during this process;MiR-17-5p was found to bind to the 3'UTR of TGFBR2 mRNA and further validation of this specific binding was performed through a reporter assay; An inverse correlation between miR-17-5p and TGFBR2 protein was observed in gastric cancer tissues; Cell studies revealed that miR-17-5p negatively regulated TGFBR2 expression by directly binding to the 3'UTR of TGFBR2 mRNA thereby promoting cell growth and migration; The results of our study suggest a novel regulatory network in gastric cancer mediated by miR-17-5p and TGFBR2 and may indicate that TGFBR2 could serve as a new therapeutic target in gastric cancer ; kidney renal cell cancer;gastric cancer hsa-miR-18a-5p TGFBR2 0.918028187958706 1.90229510425322e-05 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate -0.192780882131928 5.02272007870357e-12 NA NA NA hsa-miR-19a-3p TGFBR2 1.01712596153672 2.57753201450625e-07 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRNATAP -0.149425406079401 1.09582904607366e-06 NA NA NA hsa-miR-19b-3p TGFBR2 0.601279551368824 0.00016685097085251 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRNATAP -0.16137896859351 2.62064707293945e-05 NA NA NA hsa-miR-20a-5p TGFBR2 0.854326758727003 5.04256669663285e-07 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.177394018081005 6.48951647363697e-07 NA NA NA hsa-miR-21-5p TGFBR2 1.51084295924322 1.54551622173259e-34 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.236840105633096 2.41705180023436e-07 24037531 Androgen receptor and microRNA 21 axis downregulates transforming growth factor beta receptor II TGFBR2 expression in prostate cancer; Our results revealed that miR-21 suppresses TGFBR2 levels by binding to its 3'-UTR and AR signaling further potentiates this effect in both untransformed and transformed human prostate epithelial cells as well as in human prostate cancers; Manipulation of androgen signaling or the expression levels of AR or miR-21 negatively altered TGFBR2 expression in untransformed and transformed human prostate epithelial cells human prostate cancer xenografts and mouse prostate glands; Together these results suggest that the AR and miR-21 axis exerts its oncogenic effects in prostate tumors by downregulating TGFBR2 hence inhibiting the tumor-suppressive activity of TGFβ pathway prostate cancer hsa-miR-301a-3p TGFBR2 0.842910511640361 1.43446467417891e-06 -0.522706741081292 3.47206054315007e-05 miRNATAP -0.183722985598573 1.11942744399345e-07 25551793 MicroRNA 301a promotes migration and invasion by targeting TGFBR2 in human colorectal cancer; TGFBR2 was identified to be the downstream target of miR-301a; Knockdown of TGFBR2 in cells treated by miR-301a inhibitor elevated the previously abrogated migration and invasion; Our data indicated that miR-301a correlated with the metastatic and invasive ability in human colorectal cancers and miR-301a exerted its role as oncogene by targeting TGFBR2 colorectal cancer hsa-miR-454-3p TGFBR2 0.668749059573955 2.42670710515113e-06 -0.522706741081292 3.47206054315007e-05 miRNATAP -0.109462345848673 0.0115970871562665 NA NA NA hsa-miR-501-3p TGFBR2 0.999540908940094 2.77358485581974e-11 -0.522706741081292 3.47206054315007e-05 TargetScan -0.116887889145825 0.0037841236086272 NA NA NA hsa-miR-590-5p TGFBR2 -0.103355559980404 0.310027295317092 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRTarBase;PITA;miRanda;miRNATAP -0.253149374960949 3.5308611333711e-05 NA NA NA hsa-miR-92a-3p TGFBR2 0.212391012518195 0.134292268089997 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate -0.17351362748106 7.02401539230513e-05 NA NA NA hsa-miR-93-5p TGFBR2 1.39518349670953 2.84167728726712e-25 -0.522706741081292 3.47206054315007e-05 miRNAWalker2_validate;miRNATAP -0.263677361634044 7.09611188180981e-10 NA NA NA hsa-miR-26b-5p TRAF1 -1.10804856399744 4.14176110314282e-22 0.712897834079886 6.33601713412082e-09 miRNAWalker2_validate -0.146366230286123 0.00384999561270946 NA NA NA hsa-miR-30e-3p TRAF1 -1.20845647373031 3.10263633528203e-25 0.712897834079886 6.33601713412082e-09 mirMAP -0.220388103763073 7.10147510001104e-06 NA NA NA hsa-miR-378a-3p TRAF1 -1.1920055906972 3.23538871733634e-10 0.712897834079886 6.33601713412082e-09 miRNAWalker2_validate -0.124374707900819 6.92942238884749e-05 NA NA NA hsa-miR-139-5p TTK -2.11299531796557 8.16407076415038e-28 4.46695225538541 3.90954998066403e-48 miRanda -1.02753150497536 5.11914670343957e-45 NA NA NA hsa-miR-215-5p TTK -0.981544773246509 3.14452272477156e-05 4.46695225538541 3.90954998066403e-48 miRNAWalker2_validate -0.154558614985227 0.0292381161076613 NA NA NA hsa-miR-455-3p TTK -1.3998033821095 5.72010709806871e-17 4.46695225538541 3.90954998066403e-48 miRNATAP -0.457661482122706 1.91508543926432e-06 NA NA NA hsa-miR-10b-5p WNT7B 2.86931593037303 6.97409423207806e-23 -2.49986156980565 3.55477150766295e-09 mirMAP -0.274981207829556 5.13137898629338e-05 NA NA NA hsa-miR-34a-5p WNT7B 1.03694231790865 5.74864019540594e-14 -2.49986156980565 3.55477150766295e-09 mirMAP -0.871435014929295 2.29802092991709e-09 NA NA NA hsa-miR-505-3p WNT7B -1.20027021591555 1.27927500175937e-13 -2.49986156980565 3.55477150766295e-09 miRNATAP -0.343304152336908 0.00653008300029439 NA NA NA hsa-miR-664a-5p WNT7B -0.0738022124558593 0.597681758599428 -2.49986156980565 3.55477150766295e-09 MirTarget -0.350084715832488 0.0208450119464205 NA NA NA