miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-30d-3p ATM -0.120562286038759 0.329549534782125 -0.236058797850924 0.0173776926910908 mirMAP -0.138156674894502 0.00042794166032829 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-339-5p ATM 0.280589919878422 0.0355660282647215 -0.236058797850924 0.0173776926910908 miRanda -0.1044932927788 0.00399236512159519 NA NA NA hsa-miR-455-5p ATM -0.270546034117157 0.0581320703577731 -0.236058797850924 0.0173776926910908 miRanda -0.118715671502599 0.000450782297458542 NA NA NA hsa-miR-30a-5p BAX -0.626257932887855 0.000108198196973765 0.800291917132606 7.28210427550269e-13 miRNAWalker2_validate -0.117791332970104 0.00058384847557231 NA NA NA hsa-miR-365a-3p BAX 0.164591326307714 0.15325084963213 0.800291917132606 7.28210427550269e-13 miRNAWalker2_validate -0.229448386158489 2.12152243741233e-06 24216611 MiR 365 induces gemcitabine resistance in pancreatic cancer cells by targeting the adaptor protein SHC1 and pro apoptotic regulator BAX drug resistance pancreatic cancer hsa-let-7a-2-3p BBC3 -1.19254913456912 1.47840144520517e-08 0.804667864310401 1.64815736901645e-10 MirTarget -0.186942417061404 1.03036799278075e-10 NA NA NA hsa-let-7g-3p BBC3 -1.14091000722297 8.32736018188759e-19 0.804667864310401 1.64815736901645e-10 MirTarget;miRNATAP -0.18136207274857 9.72621187480785e-05 NA NA NA hsa-miR-101-3p BBC3 -1.48176407980989 3.98950707385704e-31 0.804667864310401 1.64815736901645e-10 miRNATAP -0.315666699732468 1.33584521433787e-12 NA NA NA hsa-miR-125b-5p BBC3 -1.35767721102906 1.16564617395164e-18 0.804667864310401 1.64815736901645e-10 miRNAWalker2_validate;miRTarBase -0.252345623766095 4.02206333883365e-11 25184537 Thus far two of these target genes BBC3 and NEU1 that are tumor suppressor genes but not yet studied in PDAC appear to be functional targets of miR-125b since knockdown of miR125b caused their up regulation pancreatic cancer hsa-miR-139-5p BBC3 -2.11299531796557 8.16407076415038e-28 0.804667864310401 1.64815736901645e-10 miRNATAP -0.234947411363537 1.07629281857681e-15 NA NA NA hsa-miR-140-5p BBC3 -0.220106443070144 0.0140713197101242 0.804667864310401 1.64815736901645e-10 miRNATAP -0.254965831183043 0.0003006053009954 NA NA NA hsa-miR-144-3p BBC3 -2.97536914478815 6.00546047309683e-28 0.804667864310401 1.64815736901645e-10 miRNATAP -0.108503369572842 3.4984805094146e-07 NA NA NA hsa-miR-27b-3p BBC3 -0.823491817819754 9.20935302746823e-14 0.804667864310401 1.64815736901645e-10 miRNATAP -0.203869768027322 0.000220938302295931 NA NA NA hsa-miR-345-5p BBC3 -0.712725883885838 3.60393522979373e-06 0.804667864310401 1.64815736901645e-10 miRNATAP -0.16515567390068 3.92597191776169e-05 NA NA NA hsa-miR-590-3p BBC3 -0.473167498047808 1.76652934637227e-05 0.804667864310401 1.64815736901645e-10 miRanda -0.254952412597708 6.04583102362517e-06 NA NA NA hsa-miR-140-5p BID -0.220106443070144 0.0140713197101242 0.212028711841568 0.0751625972679542 miRanda -0.214836635124566 0.00100941241708294 NA NA NA hsa-let-7g-5p CASP3 -0.461294133285527 2.17210114729901e-05 0.309149151776334 0.000465712323457523 MirTarget;miRNATAP -0.107118384912517 0.00693887247949783 NA NA NA hsa-miR-374b-5p CASP3 -0.307538028546424 0.0030079617545972 0.309149151776334 0.000465712323457523 mirMAP -0.143675176440622 0.00057864418465262 NA NA NA hsa-miR-455-5p CASP8 -0.270546034117157 0.0581320703577731 0.329003138913902 0.000294949473828384 miRanda -0.154954229285754 5.1724046517642e-07 NA NA NA hsa-miR-542-3p CASP8 -1.31351015149842 3.64642423288576e-22 0.329003138913902 0.000294949473828384 miRanda -0.128105307061659 3.53556200230067e-05 NA NA NA hsa-let-7b-5p CCNB1 -0.959013717270803 5.95127281258643e-11 3.15540953438863 5.73583669464518e-46 miRNAWalker2_validate -0.536702047843919 1.21768540248182e-11 NA NA NA hsa-miR-139-5p CCNB1 -2.11299531796557 8.16407076415038e-28 3.15540953438863 5.73583669464518e-46 miRanda -0.800903870286373 3.33153076219979e-55 NA NA NA hsa-let-7a-5p CCNB2 -0.328069937201898 0.000464344093744051 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.449095168142698 0.00713886337518027 NA NA NA hsa-let-7b-5p CCNB2 -0.959013717270803 5.95127281258643e-11 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.589177166241791 8.39990492744342e-09 NA NA NA hsa-let-7c-5p CCNB2 -1.70744661345097 2.87934212005942e-20 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.85447689630769 1.64053136609545e-30 NA NA NA hsa-miR-23b-3p CCNB2 -0.530561587109023 5.43807206823652e-07 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.641555640172717 9.69421048739327e-06 NA NA NA hsa-miR-339-5p CCNB3 0.280589919878422 0.0355660282647215 0.193558872127947 0.274836388284752 miRanda -0.205669923205531 0.00149302963795871 NA NA NA hsa-miR-106a-5p CCND1 -0.464229535269236 0.009724944358047 -0.901747452525989 1.22363850855738e-05 MirTarget;miRNATAP -0.262282596122017 2.76721397734651e-06 NA NA NA hsa-miR-106b-5p CCND1 0.648901865047099 8.24870862047885e-09 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.430796355484411 1.07010119372681e-06 NA NA NA hsa-miR-1266-5p CCND1 1.6344248374643 4.09278929749906e-13 -0.901747452525989 1.22363850855738e-05 MirTarget -0.234489057674296 5.64471200142146e-08 NA NA NA hsa-miR-15b-5p CCND1 0.230566941413564 0.0824765001857479 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.544455359613115 3.17021042565567e-13 NA NA NA hsa-miR-16-5p CCND1 -0.398577235179603 0.000101598495180191 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.309506657035983 0.00178218906910022 23991964;22922827;18483394 At the molecular level our results further revealed that cyclin D1 expression was negatively regulated by miR-16;CCND1 has been found to be a target of miR-15a and miR-16-1 through analysis of complementary sequences between microRNAs and CCND1 mRNA; Moreover the transcription of CCND1 is suppressed by miR-15a and miR-16-1 via direct binding to the CCND1 3'-untranslated region 3'-UTR;Truncation in CCND1 mRNA alters miR 16 1 regulation in mantle cell lymphoma; Furthermore we demonstrated that this truncation alters miR-16-1 binding sites and through the use of reporter constructs we were able to show that miR-16-1 regulates CCND1 mRNA expression; This study introduces the role of miR-16-1 in the regulation of CCND1 in MCL ;; bladder cancer;sarcoma;lymphoma hsa-miR-17-5p CCND1 0.698861147045461 2.43854106455398e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.343463418912801 9.97512783987264e-09 26431674 Bioinformatics Prediction and In Vitro Analysis Revealed That miR 17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells; In this study using bioinformatic analyses miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score; After lentiviral transduction of miR-17 to the target cells gene expression analysis showed decreased expression of CCND1 gene breast cancer hsa-miR-186-5p CCND1 -0.060103391373687 0.535288717081205 -0.901747452525989 1.22363850855738e-05 mirMAP -0.315936974513477 0.00286438511586306 NA NA NA hsa-miR-19a-3p CCND1 1.01712596153672 2.57753201450625e-07 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.283475432466515 1.38758823633513e-08 25985117 Moreover miR-19a might play inhibitory roles in HCC malignancy via regulating Cyclin D1 expression liver cancer hsa-miR-19b-1-5p CCND1 -0.277804782598538 0.0783067794722916 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase -0.307712037252966 1.4364183273616e-06 NA NA NA hsa-miR-19b-3p CCND1 0.601279551368824 0.00016685097085251 -0.901747452525989 1.22363850855738e-05 miRNATAP -0.340975400639976 4.6454998539434e-08 NA NA NA hsa-miR-20a-5p CCND1 0.854326758727003 5.04256669663285e-07 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.332506713675419 1.04479750525145e-08 NA NA NA hsa-miR-20b-5p CCND1 0.457668023910379 0.0285865211936156 -0.901747452525989 1.22363850855738e-05 MirTarget;miRNATAP -0.230429652941375 1.603682834845e-06 NA NA NA hsa-miR-340-5p CCND1 -0.00477614531381576 0.968502722773791 -0.901747452525989 1.22363850855738e-05 mirMAP -0.322810275002772 0.000125999869952101 NA NA NA hsa-miR-425-5p CCND1 0.585974239943864 2.23063410931402e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.385144136522737 9.38557217850495e-08 NA NA NA hsa-miR-503-5p CCND1 0.191949282338049 0.268420664548092 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget -0.155187994375291 0.00815133470022587 26047605;23731275 MiR 503 inhibited cell proliferation of human breast cancer cells by suppressing CCND1 expression; Overexpression of miR-503 in breast cancer cell lines reduced cell proliferation through inducing G0/G1 cell cycle arrest by targeting CCND1;MicroRNA 503 suppresses proliferation and cell cycle progression of endometrioid endometrial cancer by negatively regulating cyclin D1; CCND1 has a binding sequence of miR-503 within its 3' untranslated region and was confirmed to be a direct target of miR-503 by the fluorescent reporter assays; Increasing the miR-503 level in EEC cells suppressed cell viability colon formation activity and cell-cycle progression and the inhibited oncogenic phenotypes induced by miR-503 were alleviated by ectopic expression of CCND1 without the untranslated region sequence; Collectively this study suggested that miR-503 plays a tumor-suppressor role by targeting CCND1; Abnormal suppression of miR-503 leads to an increase in the CCND1 level which may promote carcinogenesis and progression of EEC ;progression;tumorigenesis breast cancer;endometrial cancer hsa-miR-589-3p CCND1 1.16846704028095 5.88525574424201e-11 -0.901747452525989 1.22363850855738e-05 MirTarget -0.183119037529565 0.00124255651400669 NA NA NA hsa-miR-616-5p CCND1 0.150569904638223 0.402836556304524 -0.901747452525989 1.22363850855738e-05 mirMAP -0.256039244652268 5.24430962347992e-06 NA NA NA hsa-miR-7-1-3p CCND1 -0.569105143287799 1.85297665041424e-05 -0.901747452525989 1.22363850855738e-05 mirMAP -0.260985322289835 0.000573950021646959 NA NA NA hsa-miR-9-5p CCND1 1.26407994492898 8.53156156439881e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.136746717035529 1.04306605002938e-05 NA NA NA hsa-miR-92a-3p CCND1 0.212391012518195 0.134292268089997 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.407898716180535 8.06773614790968e-09 NA NA NA hsa-miR-93-5p CCND1 1.39518349670953 2.84167728726712e-25 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.335918242999821 2.01286203755089e-06 NA NA NA hsa-miR-942-5p CCND1 0.346451845105263 0.0283255399972342 -0.901747452525989 1.22363850855738e-05 MirTarget -0.245807225430864 0.000123103109119178 NA NA NA hsa-miR-130b-5p CCND2 0.170599813152408 0.337611586432771 0.356451160382274 0.0365641518216489 mirMAP -0.174593683703476 0.000182311423622 NA NA NA hsa-miR-20a-5p CCND2 0.854326758727003 5.04256669663285e-07 0.356451160382274 0.0365641518216489 miRNAWalker2_validate;miRTarBase;miRNATAP -0.156120931730772 0.0012098497067071 NA NA NA hsa-miR-28-5p CCND2 -0.430964830599964 2.67367257252925e-06 0.356451160382274 0.0365641518216489 miRanda -0.482733599739849 7.0104175669049e-08 NA NA NA hsa-miR-33a-3p CCND2 -0.683025859637737 1.01106097756419e-05 0.356451160382274 0.0365641518216489 MirTarget -0.263538375258973 6.17940317063527e-07 NA NA NA hsa-miR-3607-3p CCND2 -2.15518588554493 1.03390728226728e-23 0.356451160382274 0.0365641518216489 mirMAP -0.12362922199284 0.000700039964538526 NA NA NA hsa-miR-378a-3p CCND2 -1.1920055906972 3.23538871733634e-10 0.356451160382274 0.0365641518216489 miRNAWalker2_validate -0.183441253857915 1.70039721501862e-05 NA NA NA hsa-miR-548v CCND2 -0.27258775900243 0.176259917595269 0.356451160382274 0.0365641518216489 MirTarget -0.149460521918892 0.000339780804187451 NA NA NA hsa-miR-616-5p CCND2 0.150569904638223 0.402836556304524 0.356451160382274 0.0365641518216489 mirMAP -0.315443515897926 3.35063962863299e-12 NA NA NA hsa-miR-618 CCND2 0.13539895350352 0.517145913114103 0.356451160382274 0.0365641518216489 mirMAP -0.225947558771367 2.79586020953898e-08 NA NA NA hsa-miR-27b-3p CCND3 -0.823491817819754 9.20935302746823e-14 0.0767102129984094 0.478427267753328 miRNAWalker2_validate -0.236832359235505 2.50262257150465e-07 NA NA NA hsa-miR-320a CCND3 0.325285936317332 0.0221415065838824 0.0767102129984094 0.478427267753328 miRanda -0.118523664136384 0.00134906533817398 NA NA NA hsa-miR-125b-5p CCNE1 -1.35767721102906 1.16564617395164e-18 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate -0.798490415823305 1.91419546517386e-20 NA NA NA hsa-miR-192-5p CCNE1 -0.50188499512787 0.00344713341196035 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate -0.353990461529605 2.35568212305901e-05 NA NA NA hsa-miR-195-5p CCNE1 -1.85907534356434 1.47434041676402e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;MirTarget;miRNATAP -0.296870668934945 3.50489012043558e-05 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.963792031426845 1.83360999387345e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;miRNATAP -0.598523781447039 1.7327978808332e-05 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-26b-5p CCNE1 -1.10804856399744 4.14176110314282e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;miRNATAP -0.886251741849761 3.83249719633593e-14 NA NA NA hsa-miR-424-5p CCNE1 -2.62653257498508 2.38997974737991e-50 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.365785229181321 3.5890870773903e-07 NA NA NA hsa-miR-497-5p CCNE1 -1.41371173021948 7.09278946477743e-17 3.04640353472202 4.5986250804531e-28 MirTarget;miRNATAP -0.265241585556134 0.00124914232960422 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-let-7b-3p CCNE2 -1.21979213547786 5.84575581584603e-17 2.01834184083791 5.42527234853282e-21 mirMAP -0.268569886454063 0.000212358107274223 NA NA NA hsa-miR-126-3p CCNE2 -0.652349350198172 9.37876280344772e-09 2.01834184083791 5.42527234853282e-21 miRNAWalker2_validate -0.385200741422764 5.31716743581417e-05 NA NA NA hsa-miR-26a-5p CCNE2 -0.963792031426845 1.83360999387345e-22 2.01834184083791 5.42527234853282e-21 miRNAWalker2_validate;miRTarBase;miRNATAP -0.488121351666366 3.8244940657208e-06 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-26b-5p CCNE2 -1.10804856399744 4.14176110314282e-22 2.01834184083791 5.42527234853282e-21 miRNATAP -0.579195472860141 1.12131120131545e-10 NA NA NA hsa-miR-30a-5p CCNE2 -0.626257932887855 0.000108198196973765 2.01834184083791 5.42527234853282e-21 miRNATAP -0.356376866932366 9.6729026873686e-08 NA NA NA hsa-let-7e-5p CCNG1 0.0350298755544731 0.811068614727804 -0.0461189096804278 0.640330790441284 miRNAWalker2_validate -0.20358478922057 2.6070187268066e-10 NA NA NA hsa-miR-132-3p CCNG1 0.323796239695997 0.00272093067935091 -0.0461189096804278 0.640330790441284 MirTarget -0.192885004183872 1.30455410377392e-05 NA NA NA hsa-miR-142-3p CCNG1 -1.4243101967815 1.92609481097765e-14 -0.0461189096804278 0.640330790441284 miRanda -0.11793216563835 1.79495858391837e-06 NA NA NA hsa-miR-142-5p CCNG1 -1.44575553250033 1.18797044680592e-13 -0.0461189096804278 0.640330790441284 mirMAP -0.12112138936527 2.75470710799215e-07 NA NA NA hsa-miR-181a-5p CCNG1 0.250113435902954 0.0551933249319293 -0.0461189096804278 0.640330790441284 miRNAWalker2_validate -0.218994113425088 1.51341663088512e-09 NA NA NA hsa-miR-21-5p CCNG1 1.51084295924322 1.54551622173259e-34 -0.0461189096804278 0.640330790441284 miRNAWalker2_validate -0.131168170626415 0.000240629548489461 NA NA NA hsa-miR-212-3p CCNG1 -0.288359631986802 0.100392159518997 -0.0461189096804278 0.640330790441284 MirTarget -0.134825038079444 6.61107770328934e-07 NA NA NA hsa-miR-23a-3p CCNG1 -0.178875974460574 0.135978273540604 -0.0461189096804278 0.640330790441284 MirTarget;miRNATAP -0.315284117285778 4.58281003139781e-16 NA NA NA hsa-miR-24-3p CCNG1 -0.257825594021966 0.00689742600215061 -0.0461189096804278 0.640330790441284 miRNAWalker2_validate -0.344966060231919 2.96537193395772e-12 NA NA NA hsa-miR-27a-3p CCNG1 -0.370807380415112 0.00875721956202713 -0.0461189096804278 0.640330790441284 MirTarget;miRNATAP -0.278947355065841 1.17573051238896e-17 NA NA NA hsa-miR-339-5p CCNG1 0.280589919878422 0.0355660282647215 -0.0461189096804278 0.640330790441284 miRanda -0.176949461005405 6.83799237948816e-07 NA NA NA hsa-miR-590-5p CCNG1 -0.103355559980404 0.310027295317092 -0.0461189096804278 0.640330790441284 miRanda -0.180361786863793 0.000140331975748021 NA NA NA hsa-miR-101-3p CCNG2 -1.48176407980989 3.98950707385704e-31 0.198342536240394 0.0998646636491728 miRNATAP -0.145457107315971 0.000642902847642846 NA NA NA hsa-miR-139-5p CCNG2 -2.11299531796557 8.16407076415038e-28 0.198342536240394 0.0998646636491728 miRanda -0.116371718069027 3.6666214573436e-05 NA NA NA hsa-miR-192-3p CCNG2 -0.6401165114007 0.000266272092403814 0.198342536240394 0.0998646636491728 MirTarget -0.172909903443718 1.39509815706713e-07 NA NA NA hsa-miR-365a-3p CCNG2 0.164591326307714 0.15325084963213 0.198342536240394 0.0998646636491728 MirTarget -0.201450011619957 7.88086600572677e-05 NA NA NA hsa-miR-374b-5p CCNG2 -0.307538028546424 0.0030079617545972 0.198342536240394 0.0998646636491728 mirMAP -0.158790308561059 0.00526435142157099 NA NA NA hsa-miR-590-5p CCNG2 -0.103355559980404 0.310027295317092 0.198342536240394 0.0998646636491728 mirMAP -0.168645585099817 0.0037697797931047 NA NA NA hsa-miR-362-3p CD82 0.814007335895913 2.00108618836568e-09 -1.0957760381686 3.58989800683902e-13 miRanda -0.151572922980181 0.00571550685495377 25652145 Anti miR 362 3p Inhibits Migration and Invasion of Human Gastric Cancer Cells by Its Target CD82; Next we analyzed the level of miR-362-3p expression and CD82 in different differentiated GC cells compared with a normal gastric mucosa cell by RT-PCR and Western blot; Dual-luciferase reporter assay and Western blot confirmed a direct interaction between miR-362-3p and CD82 3'UTR; After miR-362-3p and CD82 were silenced in GC cells we compared the transfected GC cells migration and invasion capacity by transwell assay; Western blot was used to detect the impact of CD82 and miR-362-3p on epithelial-to-mesenchymal transition markers in treated GC cells; Level of miR-362-3p expression was much higher in GC cells than in normal gastric mucosa cell and miR-362-3p expression negatively correlated with CD82 mRNA expression in these cell lines; Furthermore miR-362-3p expression induced corrected GC cell metastasis capacity by suppression of CD82 expression; This study illuminated that downregulation of miR-362-3p along with the upregulation of CD82 in GC cells resulted in the inhibition of GC migration and invasion; Thus our results suggested that miR-362-3p or CD82 can be exploited as a new potential target for control of GC in the future metastasis gastric cancer hsa-miR-122-5p CDK4 -1.23907441373955 4.42181244888642e-06 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate -0.116994239033144 4.65868213756134e-11 NA NA NA hsa-miR-145-5p CDK4 -1.4795885711477 6.47308361543414e-16 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate;miRTarBase -0.153089865179612 2.59222750442516e-09 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-193b-3p CDK4 -0.166697207281748 0.272022543069482 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate -0.152018771778108 2.97527104247713e-06 NA NA NA hsa-miR-195-5p CDK4 -1.85907534356434 1.47434041676402e-22 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate;miRTarBase -0.181976803431634 3.34979886785679e-14 NA NA NA hsa-let-7a-3p CDK6 -0.568717557554068 1.00947202557819e-07 -0.306731100792328 0.220566158555725 miRNATAP -0.341190533180457 0.00261304127571301 NA NA NA hsa-let-7b-5p CDK6 -0.959013717270803 5.95127281258643e-11 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.21783266130525 0.0075638804735268 NA NA NA hsa-miR-106a-5p CDK6 -0.464229535269236 0.009724944358047 -0.306731100792328 0.220566158555725 mirMAP -0.278977768977315 3.5360030094147e-05 NA NA NA hsa-miR-106b-5p CDK6 0.648901865047099 8.24870862047885e-09 -0.306731100792328 0.220566158555725 mirMAP -0.387039073753013 0.000291052086223802 NA NA NA hsa-miR-141-3p CDK6 -0.354316381352484 0.256996894288799 -0.306731100792328 0.220566158555725 TargetScan;miRNATAP -0.140574578703219 0.000314373241862064 NA NA NA hsa-miR-148b-3p CDK6 0.266862251671911 0.00185271302045935 -0.306731100792328 0.220566158555725 mirMAP -0.757154003587567 1.00024492805767e-07 NA NA NA hsa-miR-16-5p CDK6 -0.398577235179603 0.000101598495180191 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.498098719613092 2.63556306703195e-05 NA NA NA hsa-miR-17-5p CDK6 0.698861147045461 2.43854106455398e-05 -0.306731100792328 0.220566158555725 TargetScan;mirMAP -0.333266464458022 4.3415707047215e-06 NA NA NA hsa-miR-182-5p CDK6 1.96601340710222 4.39326175603633e-09 -0.306731100792328 0.220566158555725 mirMAP -0.10653370302744 0.0027913600827753 NA NA NA hsa-miR-195-5p CDK6 -1.85907534356434 1.47434041676402e-22 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.257207165713251 2.04308440041189e-05 23333942 Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378 respectively gastric cancer hsa-miR-200c-3p CDK6 -0.104675615392797 0.716957669484981 -0.306731100792328 0.220566158555725 mirMAP -0.120071361082355 0.00446253530887124 NA NA NA hsa-miR-20a-5p CDK6 0.854326758727003 5.04256669663285e-07 -0.306731100792328 0.220566158555725 mirMAP -0.336108073900107 1.71022894952879e-06 NA NA NA hsa-miR-20b-5p CDK6 0.457668023910379 0.0285865211936156 -0.306731100792328 0.220566158555725 mirMAP -0.209722611209641 0.000304623744169473 26166554 The transfection of miR-20b into EJ cells induced G1 phase cell cycle arrest via the decreased expression of cyclin D1 CDK2 and CDK6 without affecting another G1 phase cell cycle regulator cyclin E bladder cancer hsa-miR-217 CDK6 1.05797297540381 0.0401026692682422 -0.306731100792328 0.220566158555725 mirMAP -0.175237317848738 1.85593116207955e-14 NA NA NA hsa-miR-224-3p CDK6 1.40770116795301 1.31426607144612e-08 -0.306731100792328 0.220566158555725 mirMAP -0.144127925871375 0.00324265854216547 NA NA NA hsa-miR-26a-5p CDK6 -0.963792031426845 1.83360999387345e-22 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase;miRNATAP -0.450700287383469 0.000126142178652791 26314438 Maxvision immunohistochemistry technique was used to detect the expression level of CDK6 and miR-26a in tissue of 20 ENKTCL cases 10 cases of proliferative lymphadenitis and 10 samples of normal lymph node respectively; The possible role of miR-26a and its target gene CDK6 in genesis and development of ENKTCL were analyzed according to the clinical features of ENKTCL patients; Correlation analysis showed that there was significant negative correlation between miR-26a expression and CDK6 expression r = -0.54 P = 0.04 lymphoma hsa-miR-338-3p CDK6 0.543135607704581 0.00461167747143826 -0.306731100792328 0.220566158555725 mirMAP -0.192008504458263 0.00245323065472347 NA NA NA hsa-miR-340-5p CDK6 -0.00477614531381576 0.968502722773791 -0.306731100792328 0.220566158555725 mirMAP -0.591488535627004 3.30562522942768e-09 NA NA NA hsa-miR-425-5p CDK6 0.585974239943864 2.23063410931402e-05 -0.306731100792328 0.220566158555725 mirMAP -0.371054247671967 2.11644953421021e-05 NA NA NA hsa-miR-452-5p CDK6 1.91544498799508 1.81995982853313e-14 -0.306731100792328 0.220566158555725 mirMAP -0.148489463562499 0.00161659837456088 NA NA NA hsa-miR-497-5p CDK6 -1.41371173021948 7.09278946477743e-17 -0.306731100792328 0.220566158555725 miRNATAP -0.339765827186355 7.37877046693881e-07 NA NA NA hsa-miR-502-3p CDK6 0.659598854007546 3.01316453996423e-08 -0.306731100792328 0.220566158555725 PITA;miRNATAP -0.485378420730533 1.35374384457666e-06 NA NA NA hsa-miR-616-5p CDK6 0.150569904638223 0.402836556304524 -0.306731100792328 0.220566158555725 mirMAP -0.218127986999019 0.00133578296465318 NA NA NA hsa-miR-885-5p CDK6 -0.940994379567566 0.00118729865914986 -0.306731100792328 0.220566158555725 mirMAP -0.312653333627933 1.15118921616921e-14 NA NA NA hsa-miR-92a-3p CDK6 0.212391012518195 0.134292268089997 -0.306731100792328 0.220566158555725 miRNATAP -0.478355192378327 1.89776806005731e-08 NA NA NA hsa-miR-106b-5p CDKN1A 0.648901865047099 8.24870862047885e-09 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.437829469030798 7.84136969105116e-08 NA NA NA hsa-miR-146b-5p CDKN1A 0.419719546795449 0.0457375823220155 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate -0.153153076910721 0.000589578943698456 27602131 During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis progression thyroid cancer hsa-miR-17-5p CDKN1A 0.698861147045461 2.43854106455398e-05 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.29756314131737 8.83846469129569e-08 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-20a-5p CDKN1A 0.854326758727003 5.04256669663285e-07 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.281025701374886 1.92150921220327e-07 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-423-3p CDKN1A 0.302157659154355 0.000669907339064765 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase -0.424628898441296 6.37065354057174e-05 NA NA NA hsa-miR-423-5p CDKN1A 0.698807775906485 1.02195196334612e-11 -0.769088197669944 5.80039048047741e-05 MirTarget -0.370602271693157 3.44327865138196e-05 NA NA NA hsa-miR-503-5p CDKN1A 0.191949282338049 0.268420664548092 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase -0.140430121948101 0.00973159792553366 NA NA NA hsa-miR-93-5p CDKN1A 1.39518349670953 2.84167728726712e-25 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.446611351561038 4.29858025785308e-12 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 0.346451845105263 0.0283255399972342 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate -0.264158154736877 7.79693170645745e-06 NA NA NA hsa-let-7g-5p CDKN2A -0.461294133285527 2.17210114729901e-05 3.99778785884008 5.9152054899506e-37 miRNAWalker2_validate;miRTarBase -0.477507540180217 0.00200442971450149 NA NA NA hsa-miR-125a-5p CDKN2A -0.91219969372382 1.38256246489548e-07 3.99778785884008 5.9152054899506e-37 miRanda -0.535484271218715 1.42706283082069e-08 NA NA NA hsa-miR-125b-5p CDKN2A -1.35767721102906 1.16564617395164e-18 3.99778785884008 5.9152054899506e-37 miRNAWalker2_validate -0.65479078041233 1.86032919550119e-10 23585871 In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b; Conversely treatment of prostate cancer cells with an inhibitor of miR-125b anti-miR-125b resulted in increased expression of p14ARF decreased level of Mdm2 and induction of apoptosis; In addition overexpression of miR-125b in p53-deficient PC3 cells induced down-regulation of p14ARF which leads to increased cell proliferation through a p53-independent manner prostate cancer hsa-miR-455-3p CDKN2A -1.3998033821095 5.72010709806871e-17 3.99778785884008 5.9152054899506e-37 miRNAWalker2_validate -0.424733120907109 9.98850494017421e-06 NA NA NA hsa-miR-139-5p CHEK1 -2.11299531796557 8.16407076415038e-28 1.37723795528964 1.0573870902276e-18 miRanda -0.413297733218518 4.65960798548544e-31 NA NA NA hsa-miR-195-5p CHEK1 -1.85907534356434 1.47434041676402e-22 1.37723795528964 1.0573870902276e-18 MirTarget;miRNATAP -0.271942240141891 1.81327823892101e-12 25840419 MiR 195 suppresses non small cell lung cancer by targeting CHEK1; We discovered that CHEK1 was a direct target of miR-195 which decreased CHEK1 expression in lung cancer cells lung squamous cell cancer hsa-miR-326 CHEK1 -1.881935132554 1.68087316863775e-21 1.37723795528964 1.0573870902276e-18 miRanda -0.108090613593213 0.00505181638895353 NA NA NA hsa-miR-424-5p CHEK1 -2.62653257498508 2.38997974737991e-50 1.37723795528964 1.0573870902276e-18 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.283677722756659 2.52006692893939e-13 22469983 Suppressed miR 424 expression via upregulation of target gene Chk1 contributes to the progression of cervical cancer; Moreover miR-424 expression levels were inversely correlated with Chk1 and p-Chk1 protein levels in both cervical cancer and normal tissues; Furthermore RNAi-mediated knockdown of Chk1 decreased matrix metalloproteinase 9 expression and phenocopied the tumor suppressive effects of miR-424 in cell models; Taken together our results identify a crucial tumor suppressive role of miR-424 in the progression of cervical cancer at least partly via upreglating the expression of Chk1 and p-Chk1 and suggest that miR-424 might be a candidate of prognostic predictor or an anticancer therapeutic target for cervical cancer patients progression cervical and endocervical cancer hsa-miR-497-5p CHEK1 -1.41371173021948 7.09278946477743e-17 1.37723795528964 1.0573870902276e-18 MirTarget;miRNATAP -0.288964042519174 6.26011681896352e-11 24464213 Checkpoint kinase 1 is negatively regulated by miR 497 in hepatocellular carcinoma; In silico analysis showed that CHEK1 was a candidate target of miR-497 which was previously found to be downregulated in HCC by us; To test whether miR-497 could bind to 3'untranslated region 3'UTR of CHEK1 luciferase reporter assay was conducted; The result revealed that miR-497 could bind to the 3'untranslated region 3'UTR of CHEK1 mRNA; Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1; Finally miR-497 expression was measured in the same 30 HCC samples and the correlation between miR-497 and CHEK1 was analyzed; The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1; Taken together these results demonstrated that CHEK1 was negatively regulated by miR-497 and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC which provided a potential molecular target for HCC therapy liver cancer hsa-miR-511-5p CHEK1 -1.75067415533112 3.37801163573342e-23 1.37723795528964 1.0573870902276e-18 MirTarget -0.25658486157125 8.6821122029758e-10 NA NA NA hsa-miR-542-3p CHEK2 -1.31351015149842 3.64642423288576e-22 0.994881726057935 3.38875860481421e-09 miRanda -0.175087780040737 0.0027946939746593 NA NA NA hsa-miR-361-5p CYCS 0.233202959858696 0.00961572812255327 0.261279109661729 0.0151863050548952 miRNAWalker2_validate -0.172862186931422 0.00330618553619798 NA NA NA hsa-miR-330-5p DDB2 0.442231058604099 0.0053308560843808 -0.469910670035083 0.000245821950675029 miRanda -0.145763925404756 0.000213896811082347 NA NA NA hsa-miR-27a-3p EI24 -0.370807380415112 0.00875721956202713 -0.751389455631818 2.37502680651615e-14 miRNATAP -0.123305675454532 0.000411566648826186 NA NA NA hsa-miR-330-5p EI24 0.442231058604099 0.0053308560843808 -0.751389455631818 2.37502680651615e-14 miRanda -0.133247670869592 1.65397528378139e-05 NA NA NA hsa-miR-106a-5p FAS -0.464229535269236 0.009724944358047 -1.01888565147264 1.11378826495697e-07 miRNAWalker2_validate;miRTarBase -0.302797529669468 5.79007536295077e-09 22431000;27142596 miR 106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS; Bioinformatic analysis combining with validation experiments identified FAS as a direct target of miR-106a; Moreover a significant inverse correlation was found between miR-106a and FAS expression not only in gastric cancer cell lines but also in gastric cancer specimens; Taken together these findings suggest that ectopicly overexpressed miR-106a may play an oncogenic role in gastric carcinogenesis and impair extrinsic apoptotic pathway through targeting FAS;Functional experiment ascertained that miR-106a interacted with FAS and mediated caspase3 pathway tumorigenesis; gastric cancer;gastric cancer hsa-miR-21-5p FAS 1.51084295924322 1.54551622173259e-34 -1.01888565147264 1.11378826495697e-07 miRNAWalker2_validate -0.345881974093154 8.950367188801e-07 24710931 miR 21 targets Fas ligand mediated apoptosis in breast cancer cell line MCF 7 breast cancer hsa-miR-338-3p FAS 0.543135607704581 0.00461167747143826 -1.01888565147264 1.11378826495697e-07 miRanda -0.23029320652752 2.57971450360388e-06 NA NA NA hsa-miR-361-5p FAS 0.233202959858696 0.00961572812255327 -1.01888565147264 1.11378826495697e-07 miRanda -0.400344969968845 0.000164688479523076 NA NA NA hsa-miR-590-5p FAS -0.103355559980404 0.310027295317092 -1.01888565147264 1.11378826495697e-07 miRanda -0.564263807321375 1.14802968070722e-09 NA NA NA hsa-miR-98-5p FAS -0.0487189107419432 0.715911066732548 -1.01888565147264 1.11378826495697e-07 miRNAWalker2_validate -0.184416097033268 0.00981276415174906 NA NA NA hsa-miR-148b-3p GADD45A 0.266862251671911 0.00185271302045935 -1.18438355981419 2.04421074395418e-12 MirTarget -0.53346134919236 5.63428920227987e-08 NA NA NA hsa-miR-301a-3p GADD45A 0.842910511640361 1.43446467417891e-06 -1.18438355981419 2.04421074395418e-12 MirTarget;miRNATAP -0.429003570363722 3.99121895415153e-21 NA NA NA hsa-miR-324-5p GADD45A 0.372933276649782 0.00592280066108904 -1.18438355981419 2.04421074395418e-12 miRanda -0.518772278708973 4.69587473312867e-18 NA NA NA hsa-miR-331-5p GADD45A 0.213759185384356 0.120772399478646 -1.18438355981419 2.04421074395418e-12 PITA;miRNATAP -0.430187189615618 9.35766561284238e-13 NA NA NA hsa-miR-421 GADD45A 0.941856334694081 6.12167297054077e-08 -1.18438355981419 2.04421074395418e-12 miRanda -0.399146316642953 4.95904028323981e-18 NA NA NA hsa-miR-429 GADD45A -1.40262535827285 6.93982219171912e-05 -1.18438355981419 2.04421074395418e-12 miRNATAP -0.147502825337448 3.86036414101022e-10 NA NA NA hsa-miR-454-3p GADD45A 0.668749059573955 2.42670710515113e-06 -1.18438355981419 2.04421074395418e-12 MirTarget;miRNATAP -0.336757743574097 6.70178941647605e-09 NA NA NA hsa-miR-500a-5p GADD45A 0.797906382969156 9.15958171084226e-08 -1.18438355981419 2.04421074395418e-12 MirTarget;miRNATAP -0.380400118148641 2.78037785058895e-12 NA NA NA hsa-miR-500b-5p GADD45A 0.79864276129988 8.80934488971056e-08 -1.18438355981419 2.04421074395418e-12 MirTarget -0.380385448906191 2.83925177775155e-12 NA NA NA hsa-miR-502-5p GADD45A 0.892373335336396 1.7122694518919e-06 -1.18438355981419 2.04421074395418e-12 MirTarget;PITA;miRNATAP -0.211861066175866 2.8783697640044e-06 NA NA NA hsa-miR-324-3p GADD45B 0.260348469146494 0.0506141704203545 -2.18315196507153 1.75545549044514e-24 MirTarget;miRNATAP -0.489337683262879 2.36967761576399e-09 NA NA NA hsa-miR-181a-5p GADD45G 0.250113435902954 0.0551933249319293 -1.8078339802055 7.96436141465682e-13 miRNAWalker2_validate -0.541338211358986 1.77478587318898e-08 NA NA NA hsa-miR-320a GADD45G 0.325285936317332 0.0221415065838824 -1.8078339802055 7.96436141465682e-13 miRanda -0.463178177312941 1.50939059891479e-07 NA NA NA hsa-miR-320b GADD45G 0.0919652022571331 0.607981643616945 -1.8078339802055 7.96436141465682e-13 miRanda -0.326039418271956 3.89750223667119e-06 NA NA NA hsa-miR-421 GADD45G 0.941856334694081 6.12167297054077e-08 -1.8078339802055 7.96436141465682e-13 miRanda -0.556853667845386 1.3427336009444e-15 NA NA NA hsa-miR-429 GADD45G -1.40262535827285 6.93982219171912e-05 -1.8078339802055 7.96436141465682e-13 miRNATAP -0.116320782056629 0.00122761610181022 NA NA NA hsa-miR-502-5p GADD45G 0.892373335336396 1.7122694518919e-06 -1.8078339802055 7.96436141465682e-13 miRNATAP -0.210942956888822 0.00206657080418105 NA NA NA hsa-miR-139-5p GTSE1 -2.11299531796557 8.16407076415038e-28 3.36484553481514 9.01399399431822e-38 miRanda -0.852470510147384 1.24510618616795e-44 NA NA NA hsa-miR-103a-2-5p IGF1 1.17085533927423 5.31418969120877e-09 -3.09093589123239 2.87389370715384e-21 mirMAP -0.263504392304743 0.00223342792049484 NA NA NA hsa-miR-15b-3p IGF1 0.238105490431193 0.0997721500357416 -3.09093589123239 2.87389370715384e-21 mirMAP -0.317736514699582 0.00624882495536213 NA NA NA hsa-miR-181a-5p IGF1 0.250113435902954 0.0551933249319293 -3.09093589123239 2.87389370715384e-21 mirMAP -0.504463915639783 8.50109868625984e-05 NA NA NA hsa-miR-181b-5p IGF1 0.488256489401108 0.00105321129419701 -3.09093589123239 2.87389370715384e-21 mirMAP -0.623738116833257 1.69561535563835e-08 NA NA NA hsa-miR-19a-3p IGF1 1.01712596153672 2.57753201450625e-07 -3.09093589123239 2.87389370715384e-21 MirTarget -0.400144846493838 1.38857829477185e-06 NA NA NA hsa-miR-19b-3p IGF1 0.601279551368824 0.00016685097085251 -3.09093589123239 2.87389370715384e-21 MirTarget -0.48361890440052 2.97870836726975e-06 NA NA NA hsa-miR-20a-3p IGF1 -0.318177901603857 0.0467885456739855 -3.09093589123239 2.87389370715384e-21 mirMAP -0.361896843485159 0.00054680673837891 NA NA NA hsa-miR-301a-3p IGF1 0.842910511640361 1.43446467417891e-06 -3.09093589123239 2.87389370715384e-21 MirTarget -0.644215183857533 3.25465618219484e-12 NA NA NA hsa-miR-3913-5p IGF1 0.247553671224336 0.062795011081188 -3.09093589123239 2.87389370715384e-21 mirMAP -0.347700367785319 0.00609623314715803 NA NA NA hsa-miR-421 IGF1 0.941856334694081 6.12167297054077e-08 -3.09093589123239 2.87389370715384e-21 PITA -0.472083397878338 5.5786740474017e-07 NA NA NA hsa-miR-454-3p IGF1 0.668749059573955 2.42670710515113e-06 -3.09093589123239 2.87389370715384e-21 MirTarget -0.659716525935208 1.08682616687143e-08 NA NA NA hsa-miR-940 IGF1 0.453020092551484 0.0177054921651997 -3.09093589123239 2.87389370715384e-21 MirTarget;PITA;miRNATAP -0.258190444399689 0.00419588966919949 NA NA NA hsa-miR-224-5p IGFBP3 2.67175249172213 1.46995328399796e-18 -2.55803658929415 1.7955269745611e-33 MirTarget -0.174025915145798 6.44207387905941e-07 NA NA NA hsa-miR-34a-5p IGFBP3 1.03694231790865 5.74864019540594e-14 -2.55803658929415 1.7955269745611e-33 miRNATAP -0.464959768400917 2.32405323398958e-09 NA NA NA hsa-miR-148b-5p MDM2 0.295777213389605 0.0255749859535438 -0.36172823237465 0.000468851696238999 mirMAP -0.126111944341174 0.000979992057303969 NA NA NA hsa-miR-181a-2-3p MDM2 -0.232005557505191 0.236404981276858 -0.36172823237465 0.000468851696238999 mirMAP -0.112388243613946 1.17734645194124e-05 NA NA NA hsa-miR-23a-3p MDM2 -0.178875974460574 0.135978273540604 -0.36172823237465 0.000468851696238999 mirMAP -0.134193900580038 0.00153318263298883 NA NA NA hsa-miR-25-3p MDM2 0.633062563556379 3.68686354834328e-10 -0.36172823237465 0.000468851696238999 miRNAWalker2_validate;miRTarBase -0.177207631206492 0.0003251640137327 NA NA NA hsa-miR-500a-5p MDM2 0.797906382969156 9.15958171084226e-08 -0.36172823237465 0.000468851696238999 mirMAP -0.143618881374049 1.52638427528629e-05 NA NA NA hsa-miR-589-3p MDM2 1.16846704028095 5.88525574424201e-11 -0.36172823237465 0.000468851696238999 mirMAP -0.123089784785846 1.18949322878182e-05 NA NA NA hsa-let-7c-5p MDM4 -1.70744661345097 2.87934212005942e-20 0.73357660128009 1.75853291398166e-12 MirTarget -0.16378523997819 4.65469630843185e-10 NA NA NA hsa-miR-125b-2-3p MDM4 -1.6609436107315 5.71534047969855e-18 0.73357660128009 1.75853291398166e-12 mirMAP -0.198697520871012 2.68085285319523e-15 NA NA NA hsa-miR-142-3p MDM4 -1.4243101967815 1.92609481097765e-14 0.73357660128009 1.75853291398166e-12 miRanda -0.105410499865093 9.09308239476568e-05 NA NA NA hsa-miR-144-3p MDM4 -2.97536914478815 6.00546047309683e-28 0.73357660128009 1.75853291398166e-12 miRNATAP -0.107660843247821 8.50061365782608e-10 NA NA NA hsa-miR-152-3p MDM4 -0.959123731390966 3.24700662487287e-10 0.73357660128009 1.75853291398166e-12 MirTarget -0.119998914688611 0.000316970245935102 NA NA NA hsa-miR-29a-5p MDM4 -0.113555908626946 0.349622266470788 0.73357660128009 1.75853291398166e-12 mirMAP -0.150047613316855 0.000486995064069191 NA NA NA hsa-miR-30a-3p MDM4 -1.52720594401486 1.3830066075708e-17 0.73357660128009 1.75853291398166e-12 mirMAP -0.15705344156462 1.12728759263009e-08 NA NA NA hsa-miR-30a-5p MDM4 -0.626257932887855 0.000108198196973765 0.73357660128009 1.75853291398166e-12 mirMAP;miRNATAP -0.11689161976341 0.000243624009090581 NA NA NA hsa-miR-30e-3p MDM4 -1.20845647373031 3.10263633528203e-25 0.73357660128009 1.75853291398166e-12 mirMAP -0.227459728958845 4.92469260539039e-08 NA NA NA hsa-miR-326 MDM4 -1.881935132554 1.68087316863775e-21 0.73357660128009 1.75853291398166e-12 miRanda -0.164805754022645 2.50784423018321e-11 NA NA NA hsa-miR-33b-5p MDM4 -2.28852395459234 2.69046626645242e-22 0.73357660128009 1.75853291398166e-12 MirTarget -0.112686308994984 5.88126336967115e-08 NA NA NA hsa-miR-3607-3p MDM4 -2.15518588554493 1.03390728226728e-23 0.73357660128009 1.75853291398166e-12 mirMAP -0.129449974145506 9.01215783136111e-09 NA NA NA hsa-miR-374a-3p MDM4 -0.207171354826929 0.0623494608399903 0.73357660128009 1.75853291398166e-12 mirMAP -0.166338117174665 0.000400432204456971 NA NA NA hsa-miR-374b-5p MDM4 -0.307538028546424 0.0030079617545972 0.73357660128009 1.75853291398166e-12 mirMAP -0.174608613525902 0.000521640000426174 NA NA NA hsa-miR-424-5p MDM4 -2.62653257498508 2.38997974737991e-50 0.73357660128009 1.75853291398166e-12 mirMAP -0.145693791571925 1.44293880173031e-08 NA NA NA hsa-miR-542-3p MDM4 -1.31351015149842 3.64642423288576e-22 0.73357660128009 1.75853291398166e-12 miRanda -0.117123333719277 0.00129684159331619 NA NA NA hsa-miR-204-5p PERP -0.537962150012103 0.0330895493358849 -0.726081930125131 2.85140723970197e-05 MirTarget -0.156386855015574 2.96290170711814e-06 NA NA NA hsa-miR-362-3p PERP 0.814007335895913 2.00108618836568e-09 -0.726081930125131 2.85140723970197e-05 miRanda -0.173196122188135 0.00510219598418468 NA NA NA hsa-miR-125b-5p PMAIP1 -1.35767721102906 1.16564617395164e-18 -0.747631575480281 0.00470707404300575 miRNAWalker2_validate -0.252468396382337 0.00170545577923902 NA NA NA hsa-miR-193b-3p PMAIP1 -0.166697207281748 0.272022543069482 -0.747631575480281 0.00470707404300575 miRNAWalker2_validate;MirTarget -0.334882488475395 8.39072788009579e-05 NA NA NA hsa-miR-22-3p PMAIP1 -0.628561621952407 1.33755558015523e-09 -0.747631575480281 0.00470707404300575 MirTarget -0.381734668155031 0.00195757535155073 NA NA NA hsa-miR-374a-5p PMAIP1 0.016390363747302 0.869784217438621 -0.747631575480281 0.00470707404300575 mirMAP -0.338218801729054 0.00985884295922642 NA NA NA hsa-miR-29a-3p PPM1D -0.856762511178136 4.65330336378459e-12 -0.151433823760899 0.101960501189241 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.113333253823905 0.00140193833302498 NA NA NA hsa-miR-29a-5p PPM1D -0.113555908626946 0.349622266470788 -0.151433823760899 0.101960501189241 MirTarget -0.177969381208738 1.47239168264557e-06 NA NA NA hsa-miR-29b-3p PPM1D -0.348885528720517 0.0121441341291285 -0.151433823760899 0.101960501189241 MirTarget;miRNATAP -0.185060519517486 6.44679838532992e-09 NA NA NA hsa-miR-106b-5p PTEN 0.648901865047099 8.24870862047885e-09 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;miRNATAP -0.153848683464067 0.000300263137234544 24842611;26238857;26722252 MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance;Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells cell migration;drug resistance; gastric cancer;colorectal cancer;breast cancer hsa-miR-130b-3p PTEN 0.687190305027546 0.000109068658562255 -0.546254680094508 2.77576587245444e-08 MirTarget;miRNATAP -0.10192313870156 0.000167797598748454 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-132-3p PTEN 0.323796239695997 0.00272093067935091 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.25299424939486 1.34753285647319e-08 NA NA NA hsa-miR-148b-3p PTEN 0.266862251671911 0.00185271302045935 -0.546254680094508 2.77576587245444e-08 MirTarget;miRNATAP -0.178788666527829 0.00179323404620052 NA NA NA hsa-miR-15b-3p PTEN 0.238105490431193 0.0997721500357416 -0.546254680094508 2.77576587245444e-08 mirMAP -0.176228162620837 1.2020702896027e-07 NA NA NA hsa-miR-16-2-3p PTEN -0.0336420826463706 0.805158952566973 -0.546254680094508 2.77576587245444e-08 mirMAP -0.157329566369689 9.77918616060322e-06 NA NA NA hsa-miR-181b-5p PTEN 0.488256489401108 0.00105321129419701 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;MirTarget;miRNATAP -0.114085044640472 0.000438213061284522 NA NA NA hsa-miR-186-5p PTEN -0.060103391373687 0.535288717081205 -0.546254680094508 2.77576587245444e-08 mirMAP;miRNATAP -0.166974882115203 0.000984017106604637 NA NA NA hsa-miR-193a-3p PTEN -0.117675551589896 0.309391958208578 -0.546254680094508 2.77576587245444e-08 PITA;miRanda -0.131417170977765 0.00187315114851493 26753960;23223432 Downregulation of microRNA 193 3p inhibits tumor proliferation migration and chemoresistance in human gastric cancer by regulating PTEN gene;Our study identifies miR-193a and PTEN as targets for AML1/ETO and provides evidence that links the epigenetic silencing of tumor suppressor genes miR-193a and PTEN to differentiation block of myeloid precursors drug resistance;differentiation gastric cancer;acute myeloid leukemia hsa-miR-21-5p PTEN 1.51084295924322 1.54551622173259e-34 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;mirMAP -0.235206406510716 3.9272579156044e-11 23684551;17681183;25973032;26311740;26387181;22267008;26384051;22322462;27644439;21471222;21468550;20113523;26905520;21806946;24154840;27611950;24780321;25027758;23036707;26559642;26731559;26847601;27350731;24331411;21820606;25909227;24930006;24293118;26289851;22547075;26666820;24324076;23201752;25963606;27188433;22958183;22956424;19730150;21842656;21104017;23894315;23548551;26236156;23951172;24460329;20092645;25563770;24659669;25647415;26230405;25543482;20048743;27725205;23466500;22922228;25058005;20223231;27220494;22678116;24763002;24221338;22120473;21408027;23174819;22832383;19212625;22978663;25799148;26741162;23226804;26787105;26864640;25603978;26975392 MicroRNA 21 suppresses PTEN and hSulf 1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways;MicroRNA 21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer; PTEN was shown to be a direct target of miR-21 and to contribute to miR-21 effects on cell invasion; Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9 both downstream mediators of PTEN involved in cell migration and invasion; Aberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth migration and invasion;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;Exposure of HCC cells to sorafenib led to an increase in miR-21 expression a decrease in PTEN expression and sequential Akt activation;In addition treated with 5-AZA resulted in significant increases of miR-21 expression in both MCF-7 and MDA-MB-231 cells P < 0.01 with the protein level of PTEN increased in MCF-7 cell which was further involved in the downregulation of AKT;microRNA 21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN; Thus in this study we focused on the expression and significance of miR-21 in gastric cancer tissues and the role of miR-21 in the biological behaviour and the expression of PTEN in gastric cancer cells; Western blotting and the Luciferase Reporter Assay were used to evaluate the change of PTEN expression after lowered expression of miR-21 in gastric cancer cell lines; The western blot results and Luciferase Reporter Assay demonstrated that PTEN expression was remarkably increased after miR-21 inhibition P<0.05 microRNA-21 expression was upregulated in gastric carcinoma tissues and was significantly associated with the degree of differentiation of tumour tissues local invasion and lymph node metastasis;Furthermore we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog PTEN the result of which can be rescued by miR-21 inhibitor;Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon;Bmi-1 also regulates p53 and PTEN via miR-21;Here we demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474 SKBR3 and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody whereas protein expression of the PTEN gene a miR-21 target was reduced; Rescuing PTEN expression with a p3XFLAG-PTEN-mut construct with deleted miR-21 targeting sequence at its 3' UTR restored the growth inhibition of trastuzumab in the resistant cells by inducing PTEN activation and AKT inhibition; In vivo administering miR-21 antisense oligonucleotides restored trastuzumab sensitivity in the resistant breast cancer xenografts by inducing PTEN expression whereas injection of miR-21 mimics conferred trastuzumab resistant in the sensitive breast tumors via PTEN silence;The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting respectively in tumor tissues as well as adjacent non-tumor mucosa; miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues; A negative correlation between expression of miR-21 and PTEN was established in vivo;MicroRNA 21 inhibitor sensitizes human glioblastoma cells U251 PTEN mutant and LN229 PTEN wild type to taxol; Human glioblastoma U251 PTEN-mutant and LN229 PTEN wild-type cells were treated with taxol and the miR-21 inhibitor in a poly amidoamine PAMAM dendrimer alone or in combination; Interestingly the above data suggested that in both the PTEN mutant and the wild-type GBM cells miR-21 blockage increased the chemosensitivity to taxol; Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;The effect of ZA on miR-21 expression was quantified by qRT-PCR and the amount of PTEN protein and its targets were analyzed by Western blot;NTR1 activation stimulates expression of miR-21 and miR-155 in colonocytes via Akt and NF-κB to down-regulate PTEN and SOCS1 and promote growth of tumors in mice;Correlations between the expression levels of miR-21 PTEN and p-AKT were analyzed by real-time PCR and Western blot test in HER2-positive GC cell lines; Overexpression of miR-21 down-regulated PTEN expression increased AKT phosphorylation and did not affect HER2 expression; Inversely suppression of miR-21 increased PTEN expression and down-regulated AKT phosphorylation but still did not affect HER2 expression;MicroRNA 21 promotes TGF β1 induced epithelial mesenchymal transition in gastric cancer through up regulating PTEN expression; In GC tissues the expressions of miR-21 Akt and p-Akt were up-regulated while PTEN expression was down-regulated; These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression;Expressions of PTEN were significantly down-regulated in CRC tissues and negatively correlated with expressions of Notch-1 r2=0.5207 p<0.01 and miR-21 r2=0.6996 p<0.01; These data indicate that the crosstalk between Notch-1 and miR-21 is involved in CRC development through degradation of PTEN;In the same patients we also compared miR-21 expression with the expression of its presumed target PTEN; miR-21 expression levels were found to significantly correlate with tumour size r = 0.403 p = 0.009; Spearman's rank whereas no relation was found between miR-21 and PTEN expression levels Kruskal-Wallis test;Over-expression of miR-21 suppressed its target PTEN and disrupted acinar morphogenesis;There was overexpression of the miR-21 target genes PTEN by 67% and caspase-3 by 15% upon cotreatment;We previously reported that microRNA-21 miR-21 was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target PTEN; Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN stem-loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status; miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression; These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus;Triptolide reduces proliferation and enhances apoptosis of human non small cell lung cancer cells through PTEN by targeting miR 21; To the best of our knowledge the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21;Quantitative real-time PCR qRT-PCR and Western blot were used to detect the expression levels of miR-21 and PTEN in HCT116 HT29 Colo32 and SW480 CRC cell lines; Also the expression levels of PTEN mRNA and its downstream proteins AKT and PI3K in HCT116 cells after downregulating miR-21 were investigated; In comparing the levels of PTEN protein and downstream AKT and PI3K in HCT116 cells after downregulation of miR-21 expression the levels of AKT and PI3K protein expression significantly decreased P < 0.05; PTEN is one of the direct target genes of miR-21;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;MicroRNA 21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN; TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-21 and tumor suppressor gene PTEN protein; We showed that upregulation of miR-21 in MCF-7/ADR cells was concurrent with downregulation of PTEN protein; Overexpression of PTEN could mimic the same effects of miR-21 inhibitor in MCF-7/ADR cells and PTEN-siRNA could increase the resistance of MCF-7 cells to ADR; MiR-21 inhibitor could increase PTEN protein expression and the luciferase activity of a PTEN 3' untranslated region-based reporter construct in MCF-7/ADR cells; Dysregulation of miR-21 plays critical roles in the ADR resistance of breast cancer at least in part via targeting PTEN;MiR-21 level was inversely correlated with the levels of FOXO1 and PTEN in DLBCL cell lines; MiR-21 also down-regulated PTEN expression and consequently activated the PI3K/AKT/mTOR pathway which further decreased FOXO1 expression;In present study we determined the miR-21 levels in TNBC specimens and TNBC cell levels in vitro and then identified the role of miR-21 on tumor cell proliferation apoptosis and then identified PTEN as the possible target of the microRNA;MiR 21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non small cell lung cancer A549 cells; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA was performed to modulate the expression of microRNA-21 and PTEN under the treatment of curcumin; Moreover the protein level of PTEN a putative target of microRNA-21 was significantly elevated in curcumin-treated A549 cells as determined by Western blot analysis; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA significantly P < 0.05 reversed the growth suppression and apoptosis induction by curcumin compared to corresponding controls; Our data suggest a novel molecular mechanism in which inhibition of microRNA-21 and upregulation of PTEN mediate the anticancer activities of curcumin in NSCLC cells;PTEN gene expression was performed as a known target of miR-21;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;PTEN a direct target gene of miR-21 was significantly downregulated in gemcitabine-resistant breast cancer cells and restoration of PTEN expression blocked miR-21-induced EMT and gemcitabine resistance;We also found that Rawq01 up-regulated the expression of PTEN through mir-21 inhibition and therefore inhibited the PI3K-AKT pathway;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;Overexpression of miR 21 promotes the proliferation and migration of cervical cancer cells via the inhibition of PTEN; The aim of this study was to examine the expression of miR-21 and PTEN in cervical cancer specimens using quantitative PCR; miR-21 was upregulated in the cervical cancer specimens negatively correlating with the PTEN mRNA level; Transfection of the miR-21 mimics was markedly promoted whereas the miR-21 inhibitor suppressed the proliferation migration and invasion of cervical cancer cells with a significant inhibition of PTEN expression; The present study showed the upregulation of miR-21 in invasive cervical cancers and confirmed the promotion of miR-21 with regard to the proliferation migration and invasion in cervical cancer cells via inhibiting the PTEN expression;The expressions of miR-21 PTEN PI3K and AKT were detected in 89 esophageal cancer samples and 58 adjacent normal tissues respectively; MiR-21 PI3K and AKT have higher expressions but PTEN has lower expression in esophageal cancer tissues compared with adjacent normal tissues; Further PTEN was a target gene of miR-21;The expression level of miR-21 and PTEN messenger RNA were measured by quantitative real-time reverse transcription polymerase chain reaction or reverse transcription polymerase chain reaction; miR-21 was overexpressed and PTEN was suppressed in established radioresistant TE-R60 cells compared with the parent cells 1.3-fold and 70.83%; The inhibition of miR-21 significantly increased the cells' radiosensitivity P < 0.05 and the PTEN protein expression 2.3-fold in TE-1 cells; Knockdown of PTEN in anti-miR-21 TE-1 cells could abrogate the miR-21 inhibition-induced radiosensitization P < 0.05; Inhibition of miR-21 increased radiosensitivity of esophageal cancer TE-1 cells and this effect was possibly through the activation of PTEN;MicroRNA 21 miR 21 expression promotes growth metastasis and chemo or radioresistance in non small cell lung cancer cells by targeting PTEN; Taken together these results provide evidence to show the promotion role of miR-21 in NSCLC development through modulation of the PTEN signaling pathway;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Role of microRNA 21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma; To evaluate the role of miR-21 and PTEN cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells; In Eca109 when transfected with miR-21 mimics accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40% which resulted in the promotion of cell proliferation; However when transfected with miR-21 inhibitor expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79% which resulted in the suppression of cell proliferation; Furthermore there was a significantly inverse correlation between miR-21 expression and PTEN protein levels p < 0.05; The author concluded that MiR-21 was overexpressed in vitro and ESCC and promoted the cell proliferation might target PTEN at post-transcriptional level and regulated the cancer invasion in Kazakh's ESCC;Difluorinated curcumin CDF restores PTEN expression in colon cancer cells by down regulating miR 21; Indeed our current data demonstrate a marked downregulation of PTEN in SCID mice xenografts of miR-21 over-expressing colon cancer HCT116 cells; Colonospheres that are highly enriched in cancer stem/stem like cells reveal increased miR-21 expression and decreased PTEN; Difluorinated curcumin CDF a novel analog of the dietary ingredient curcumin which has been shown to inhibit the growth of 5-Flurouracil + Oxaliplatin resistant colon cancer cells downregulated miR-21 in chemo-resistant colon cancer HCT116 and HT-29 cells and restored PTEN levels with subsequent reduction in Akt phosphorylation;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells;The results showed that knockdown of miR-21 by antagomir-21 decreased cell proliferation and induced apoptosis via targeting PTEN both in 4T1 cells and HUVECs;Finally invasion and metastasis assays were performed and alteration in mir-21 PTEN AKT and pAKT level was evaluated in these cells; Enhanced invasion and metastasis increased miR-21 expression decreased PTEN elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells;In the present study we investigated the role of miR-21 and its potential as a therapeutic target in two prostate cancer cell lines characterized by different miR-21 expression levels and PTEN gene status;Expressions of microRNA-21 miR-21 PTEN MMP9 and p47 were detected by qPCR;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;MiR-21 upregulation contributes to PTEN downregulation which is beneficial for the activation of PI3K/AKT signaling;microRNA 21 Regulates Cell Proliferation and Migration and Cross Talk with PTEN and p53 in Bladder Cancer; MicroRNA-21 regulates proliferation and migration of bladder cancer cells and cross talk with PTEN and p53 in bladder cancer;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status; To explore whether miR-21 can serve as a therapeutic target for glioblastoma we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 PTEN mutant and LN229 PTEN wild-type GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo; Taken together our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated; In conclusion our data suggest that mUA can suppress cell viability in DU145 cells through modulating miR-21 and its downstream series-wound targets including PTEN Akt and Wnt/β-catenin signaling;miR 21 confers cisplatin resistance in gastric cancer cells by regulating PTEN; In addition miR-21 induced cell survival and cisplatin resistance through downregulating the expression of phosphatase and tension homolog deleted on chromosome 10 PTEN and activation of Akt pathway;Finally we demonstrate that modulation of tumor suppressors PTEN and p53 in U87 cells does not affect the decrease in miR-21 levels associated with PDGF-B overexpression;This study aimed to investigate whether NSCLC miR-21 mediated resistance to TKIs also results from Pten targeting; Here we show miR-21 promotes cancer by negatively regulating Pten expression in human NSCLC tissues: high miR-21 expression levels were associated with shorter DFS in 47 NSCLC patients; high miR-21/low Pten expression levels indicated a poor TKI clinical response and shorter overall survival in another 46 NSCLC patients undergoing TKI treatment; In vitro assays showed that miR-21 was up-regulated concomitantly to down-regulation of Pten in pc-9/GR cells in comparison with pc-9 cells; Moreover over-expression of miR-21 significantly decreased gefitinib sensitivity by down-regulating Pten expression and activating Akt and ERK pathways in pc-9 cells while miR-21 knockdown dramatically restored gefitinib sensitivity of pc-9/GR cells by up-regulation of Pten expression and inactivation of AKT and ERK pathways in vivo and in vitro;MicroRNA 21 miR 21 represses tumor suppressor PTEN and promotes growth and invasion in non small cell lung cancer NSCLC; We identified the role of miR-21 in non-small cell lung cancer NSCLC and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation; Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot respectively; Tumor tissues showed an inverse correlation between miR-21 and PTEN protein; miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines; miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC;MiR-21 overexpression decreases PTEN increases p-Akt and subsequently increases HIF-1α expression while miR-21 inhibition results in increased PTEN decreased p-Akt and then decreased HIF-1α;Furthermore miR-21-induced upregulation of CSF-1 mRNA and its transcription were prevented by expression of PTEN mRNA lacking 3'-untranslated region UTR and miR-21 recognition sequence; Our results reveal a novel mechanism for the therapeutic function of fish oil diet that blocks miR-21 thereby increasing PTEN levels to prevent expression of CSF-1 in breast cancer;This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma DLBCL paraffin-embedded tissues and evaluate its potential relevance with clinical characteristics; In patients with DLBCL the expression level of miR-21 was negatively correlated with the level of PTEN protein; These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL;Down regulation of PTEN expression modulated by dysregulated miR 21 contributes to the progression of esophageal cancer; We demonstrated that knockdown of miR-21 significantly increased expression of PTEN protein; Our findings suggest that miR-21 could be a potential oncomiR probably by regulation of PTEN and a novel prognostic factor for ESCC patients;The levels of mir-21 did not associate with the expression of PTEN an important tumour suppressor in CRC and one of many putative targets of miR-21 but interestingly was associated with stage of disease in the PTEN expressing tumours;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants;The PTEN protein levels in CRC tissues and cells had an inverse correlation with miR-21 expression; MiR-21 targets PTEN at the post-transcriptional level and regulates cell proliferation and invasion in CRC;After miR-21 was transfected in MCF-7 cells PTEN protein level was measured by Western blot; Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt resulting in accumulation of Bad p21/WAF1/CIP1 and p27/KIP1;To further determine the potential involvement of miR-21 in breast cancer we have evaluated the expression level of miR-21 by stem-loop real-time RT-PCR based on SYBR-Green I in human invasive ductal carcinoma of the breast and we have correlated the results with clinicopathologic features and PTEN protein expression; The expression levels of miR-21 were correlated with PTEN and commonly used clinicopathologic features of breast cancer; Expression of miR-21 was negatively correlated with expression of PTEN P=0.013; These findings suggest that PTEN is possibly one of the targets of miR-21 in breast cancer and high expression of mir-21 indicates a more aggressive phenotype;These findings demonstrate a novel role of AR in the regulation of miR-21 and its target PTEN in growth factor-induced colon cancer cell growth;Whether miR-21 regulated PTEN expression was assessed by luciferase assay; The RNA and protein levels of PTEN were significantly decreased by exogenous miR-21 and the 3'-untranslated region of PTEN was shown to be a target of miR-21;In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX;microRNA 21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer; We performed a qRT-PCR assay with miR-21 and PTEN in 16 paired EEC tumor tissues and adjacent non-tumor endometrium; To validate the putative binding site of miR-21 in the 3' untranslated region 3'-UTR of PTEN messenger RNA mRNA a dual-luciferase reporter assay was carried out; The upregulation of miR-21 led to a significant decrease in the PTEN protein expression level P=0.007; The downregulation of miR-21 led to a significant increase in PTEN protein P=0.002; In conclusion we demonstrated that the expression of PTEN protein but not mRNA was negatively directly regulated by miR-21 in the KLE cell line; The overexpression of miR-21 modulated EEC cell proliferation through the downregulation of PTEN;The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR 21 and promoter methylation; In this study we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC; PTEN mRNA level was negatively correlated with miR-21 level r = -0.595 P < 0.001; PTEN expression was also correlated directly with the PTEN mRNA level r = 0.583 P < 0.001 and conversely with miR-21 level r = -0.632 P < 0.001; This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;MicroRNA 21 controls hTERT via PTEN in human colorectal cancer cell proliferation; The aim of this study was to determine a role of microRNA-21 miRNA-21 in colorectal cancer CRC and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue PTEN;Relevance of miR 21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells; We identified the tumor suppressor gene PTEN as a target of miR-21 and determined the mechanism of its regulation throughout reporter construct plasmids; Using this model we analyzed the expression of miR-21 and PTEN as well as functional effects such as autophagy and apoptosis induction; In SiHa cells there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells; We conclude that miR-21 post-transcriptionally down-regulates the expression of PTEN to promote cell proliferation and cervical cancer cell survival progression;cell migration;;;;metastasis;differentiation;;tumorigenesis;;drug resistance;;;;;;;;;;;;;;drug resistance;drug resistance;;;;;;drug resistance;;;;;;metastasis;drug resistance;;;;;;;;metastasis;;;;;;;progression;malignant trasformation;;drug resistance;poor survival;;drug resistance;poor survival;;;;;progression;staging;;;;;;;;;;drug resistance;;poor survival liver cancer;liver cancer;liver cancer;liver cancer;breast cancer;gastric cancer;cervical and endocervical cancer;colon cancer;gastric cancer;breast cancer;bladder cancer;glioblastoma;breast cancer;colon cancer;gastric cancer;gastric cancer;colorectal cancer;breast cancer;lung cancer;glioblastoma;melanoma;lung squamous cell cancer;colorectal cancer;lung squamous cell cancer;breast cancer;B cell lymphoma;breast cancer;lung squamous cell cancer;thyroid cancer;breast cancer;breast cancer;esophageal cancer;glioblastoma;cervical and endocervical cancer;esophageal cancer;esophageal cancer;lung squamous cell cancer;pancreatic cancer;lung cancer;esophageal cancer;colon cancer;B cell lymphoma;colon cancer;breast cancer;pancreatic cancer;prostate cancer;lung cancer;gastric cancer;breast cancer;bladder cancer;B cell lymphoma;glioblastoma;prostate cancer;gastric cancer;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;cervical and endocervical cancer;breast cancer;B cell lymphoma;esophageal cancer;colorectal cancer;pancreatic cancer;colorectal cancer;breast cancer;breast cancer;colon cancer;lung squamous cell cancer;lung cancer;endometrial cancer;colorectal cancer;pancreatic cancer;colorectal cancer;cervical and endocervical cancer hsa-miR-212-3p PTEN -0.288359631986802 0.100392159518997 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.126653228047854 4.35312213024863e-06 NA NA NA hsa-miR-25-3p PTEN 0.633062563556379 3.68686354834328e-10 -0.546254680094508 2.77576587245444e-08 miRTarBase;MirTarget;miRNATAP -0.17145581255423 0.000296261160928615 NA NA NA hsa-miR-425-5p PTEN 0.585974239943864 2.23063410931402e-05 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.14409935403185 3.42816202938787e-05 25154996 An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation gastric cancer hsa-miR-484 PTEN 0.0857240033709656 0.453982056282229 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.198915392224346 2.77954860077785e-06 NA NA NA hsa-miR-589-3p PTEN 1.16846704028095 5.88525574424201e-11 -0.546254680094508 2.77576587245444e-08 MirTarget;mirMAP -0.104104404805544 0.000121296389317292 NA NA NA hsa-miR-590-5p PTEN -0.103355559980404 0.310027295317092 -0.546254680094508 2.77576587245444e-08 mirMAP -0.170198210686497 0.000404726658138002 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-93-5p PTEN 1.39518349670953 2.84167728726712e-25 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;miRNATAP -0.151328205041358 8.0568838043516e-06 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-361-5p RCHY1 0.233202959858696 0.00961572812255327 -0.63700110053237 1.4609102274865e-14 MirTarget;miRanda;miRNATAP -0.201692986005002 1.35595825642858e-05 NA NA NA hsa-miR-501-3p RCHY1 0.999540908940094 2.77358485581974e-11 -0.63700110053237 1.4609102274865e-14 MirTarget -0.167655690111899 2.77468435472342e-10 NA NA NA hsa-miR-501-5p RCHY1 1.15231083477882 2.98906150004088e-10 -0.63700110053237 1.4609102274865e-14 mirMAP -0.130995819132317 2.21502164923172e-09 NA NA NA hsa-miR-502-3p RCHY1 0.659598854007546 3.01316453996423e-08 -0.63700110053237 1.4609102274865e-14 MirTarget -0.174094700947805 3.49177745849345e-07 NA NA NA hsa-miR-16-5p RFWD2 -0.398577235179603 0.000101598495180191 0.633010495593122 4.7570127100633e-15 MirTarget -0.158805600674654 5.73579614126931e-05 NA NA NA hsa-miR-186-5p RFWD2 -0.060103391373687 0.535288717081205 0.633010495593122 4.7570127100633e-15 MirTarget;miRNATAP -0.122143467058059 0.00396009418888975 NA NA NA hsa-miR-195-5p RFWD2 -1.85907534356434 1.47434041676402e-22 0.633010495593122 4.7570127100633e-15 MirTarget -0.105545894540319 1.26177871904194e-07 NA NA NA hsa-miR-24-3p RFWD2 -0.257825594021966 0.00689742600215061 0.633010495593122 4.7570127100633e-15 miRNAWalker2_validate -0.111962793666348 0.00912963003587091 NA NA NA hsa-miR-424-5p RFWD2 -2.62653257498508 2.38997974737991e-50 0.633010495593122 4.7570127100633e-15 MirTarget;miRNATAP -0.136010026133592 8.03393257116334e-12 NA NA NA hsa-miR-590-3p RFWD2 -0.473167498047808 1.76652934637227e-05 0.633010495593122 4.7570127100633e-15 miRanda -0.118839451266468 0.00120537998403123 NA NA NA hsa-miR-7-1-3p RFWD2 -0.569105143287799 1.85297665041424e-05 0.633010495593122 4.7570127100633e-15 MirTarget -0.158154092276233 1.38627662663683e-07 NA NA NA hsa-let-7a-5p RRM2 -0.328069937201898 0.000464344093744051 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate;TargetScan;miRNATAP -0.421236541054937 0.00211660207973074 NA NA NA hsa-let-7b-5p RRM2 -0.959013717270803 5.95127281258643e-11 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate;miRNATAP -0.510760525666518 1.14461203495486e-09 NA NA NA hsa-let-7g-5p RRM2 -0.461294133285527 2.17210114729901e-05 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate;miRNATAP -0.596390456218578 2.84810771394427e-07 NA NA NA hsa-miR-100-5p RRM2 -0.783576782126614 0.000219958772262677 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate -0.469986657104793 4.20233947133701e-16 NA NA NA hsa-miR-125a-5p RRM2 -0.91219969372382 1.38256246489548e-07 3.28040567179396 1.92251546747087e-44 miRanda -0.366414420072369 3.56185323552722e-07 NA NA NA hsa-miR-199a-5p RRM2 -1.98848462405923 2.0421622713753e-11 3.28040567179396 1.92251546747087e-44 miRanda -0.287593523150928 2.03574804917786e-12 NA NA NA hsa-miR-26a-5p RRM2 -0.963792031426845 1.83360999387345e-22 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate -0.776533369915346 1.48143326460146e-10 NA NA NA hsa-miR-30a-5p RRM2 -0.626257932887855 0.000108198196973765 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate -0.585729925912413 1.4849714290135e-14 NA NA NA hsa-miR-30c-5p RRM2 -0.432111642767632 0.000159764714931007 3.28040567179396 1.92251546747087e-44 miRNAWalker2_validate -0.448871386564653 5.30128645915653e-05 NA NA NA hsa-miR-26a-2-3p RRM2B -0.552429521617 7.7322049253051e-05 -0.0376797680650993 0.706493541542365 mirMAP -0.132499927341511 0.000122669433849738 NA NA NA hsa-miR-28-5p RRM2B -0.430964830599964 2.67367257252925e-06 -0.0376797680650993 0.706493541542365 miRanda -0.141384391078115 0.00761280325916041 NA NA NA hsa-miR-590-5p RRM2B -0.103355559980404 0.310027295317092 -0.0376797680650993 0.706493541542365 miRanda;mirMAP -0.198086865537509 3.60814065374783e-05 NA NA NA hsa-miR-148a-5p SERPINE1 -0.767626696027761 1.08359477494797e-06 -2.5626204615753 1.45202766560285e-19 miRNATAP -0.260590457035894 0.00415058434081855 NA NA NA hsa-miR-30d-5p SERPINE1 0.718283816348023 2.47422074061352e-08 -2.5626204615753 1.45202766560285e-19 miRNATAP -0.510774042367725 3.44422983628064e-06 NA NA NA hsa-miR-34a-5p SERPINE1 1.03694231790865 5.74864019540594e-14 -2.5626204615753 1.45202766560285e-19 miRNATAP -0.399999110948681 8.04313907897335e-05 NA NA NA hsa-miR-15b-5p SESN1 0.230566941413564 0.0824765001857479 -0.582017748855085 1.56850305646972e-06 MirTarget;miRNATAP -0.11696083174855 0.00962215473976974 NA NA NA hsa-miR-21-5p SESN1 1.51084295924322 1.54551622173259e-34 -0.582017748855085 1.56850305646972e-06 miRNAWalker2_validate -0.309095857741543 1.28235786632969e-12 NA NA NA hsa-miR-17-5p SESN2 0.698861147045461 2.43854106455398e-05 -0.10100761172075 0.366158583982995 TargetScan;miRNATAP -0.117675195510559 0.000286632286006239 NA NA NA hsa-miR-320a SESN2 0.325285936317332 0.0221415065838824 -0.10100761172075 0.366158583982995 mirMAP -0.142863640875602 0.000178407715578198 NA NA NA hsa-miR-320b SESN2 0.0919652022571331 0.607981643616945 -0.10100761172075 0.366158583982995 mirMAP -0.111417793439893 0.000249574704690751 NA NA NA hsa-miR-93-5p SESN2 1.39518349670953 2.84167728726712e-25 -0.10100761172075 0.366158583982995 miRNATAP -0.141198072246715 0.000204251868400618 NA NA NA hsa-miR-106b-5p SESN3 0.648901865047099 8.24870862047885e-09 -0.821541570789657 0.00383798636487833 MirTarget -0.335981495719137 0.00594887061637879 NA NA NA hsa-miR-130b-3p SESN3 0.687190305027546 0.000109068658562255 -0.821541570789657 0.00383798636487833 mirMAP -0.218615550647049 0.00494000758657603 NA NA NA hsa-miR-17-5p SESN3 0.698861147045461 2.43854106455398e-05 -0.821541570789657 0.00383798636487833 MirTarget;TargetScan -0.443064169218768 7.25920765515808e-08 NA NA NA hsa-miR-181a-5p SESN3 0.250113435902954 0.0551933249319293 -0.821541570789657 0.00383798636487833 MirTarget -0.319299217345204 0.00282677245748294 NA NA NA hsa-miR-181b-5p SESN3 0.488256489401108 0.00105321129419701 -0.821541570789657 0.00383798636487833 MirTarget -0.324236880289917 0.00047836521786657 NA NA NA hsa-miR-19b-1-5p SESN3 -0.277804782598538 0.0783067794722916 -0.821541570789657 0.00383798636487833 MirTarget -0.280092778145656 0.00148449459659292 NA NA NA hsa-miR-200a-5p SESN3 -1.16865179527606 0.00105385823807419 -0.821541570789657 0.00383798636487833 mirMAP -0.137250175576184 0.00038794439690191 NA NA NA hsa-miR-200b-3p SESN3 -1.29335788099229 0.000268623234305059 -0.821541570789657 0.00383798636487833 MirTarget;TargetScan -0.137355288143689 0.000388778971985253 NA NA NA hsa-miR-20a-3p SESN3 -0.318177901603857 0.0467885456739855 -0.821541570789657 0.00383798636487833 mirMAP -0.294855118924278 0.000690722772975796 NA NA NA hsa-miR-20a-5p SESN3 0.854326758727003 5.04256669663285e-07 -0.821541570789657 0.00383798636487833 MirTarget -0.40024159648166 5.55264508290224e-07 NA NA NA hsa-miR-29a-5p SESN3 -0.113555908626946 0.349622266470788 -0.821541570789657 0.00383798636487833 MirTarget;mirMAP -0.408985218236979 0.000374982809599754 NA NA NA hsa-miR-301a-3p SESN3 0.842910511640361 1.43446467417891e-06 -0.821541570789657 0.00383798636487833 mirMAP -0.313818250031627 6.02567843002784e-05 NA NA NA hsa-miR-30d-3p SESN3 -0.120562286038759 0.329549534782125 -0.821541570789657 0.00383798636487833 mirMAP -0.623969259609949 2.25998465205713e-08 NA NA NA hsa-miR-339-5p SESN3 0.280589919878422 0.0355660282647215 -0.821541570789657 0.00383798636487833 miRanda -0.357751591608863 0.000598196713057192 NA NA NA hsa-miR-33a-5p SESN3 -0.767474460958522 1.09948756118522e-05 -0.821541570789657 0.00383798636487833 mirMAP -0.323125537813582 3.85696728190764e-05 NA NA NA hsa-miR-429 SESN3 -1.40262535827285 6.93982219171912e-05 -0.821541570789657 0.00383798636487833 MirTarget -0.137981451985493 0.00047056027491648 NA NA NA hsa-miR-454-3p SESN3 0.668749059573955 2.42670710515113e-06 -0.821541570789657 0.00383798636487833 mirMAP -0.363148365863571 0.000176441387078282 NA NA NA hsa-miR-589-3p SESN3 1.16846704028095 5.88525574424201e-11 -0.821541570789657 0.00383798636487833 MirTarget;mirMAP -0.246491020384425 0.00151323326013284 NA NA NA hsa-miR-589-5p SESN3 1.18593770505086 1.01781241960531e-22 -0.821541570789657 0.00383798636487833 MirTarget -0.406565484508545 0.0001930052233513 NA NA NA hsa-miR-877-5p SESN3 1.35669625926821 1.43008125755656e-11 -0.821541570789657 0.00383798636487833 mirMAP -0.212605517598794 0.00258341841126729 NA NA NA hsa-miR-92a-3p SESN3 0.212391012518195 0.134292268089997 -0.821541570789657 0.00383798636487833 MirTarget;miRNATAP -0.389283827611037 6.99870922684295e-05 NA NA NA hsa-miR-93-5p SESN3 1.39518349670953 2.84167728726712e-25 -0.821541570789657 0.00383798636487833 MirTarget -0.441745874271197 5.18930583534045e-06 NA NA NA hsa-miR-532-5p SIAH1 1.02729546510601 9.93318913966636e-16 -0.248598766920746 0.000491682203063644 PITA;miRNATAP -0.102457696857658 9.08176167400043e-05 NA NA NA hsa-miR-107 STEAP3 0.241662461253461 0.0170788899505389 -2.25134335305349 3.22165376330082e-22 miRanda -0.402796055034589 0.000682593818150116 NA NA NA hsa-miR-188-5p STEAP3 1.11646899351496 9.56686958408598e-11 -2.25134335305349 3.22165376330082e-22 mirMAP -0.368509057306644 3.82878475901557e-08 NA NA NA hsa-miR-222-5p STEAP3 0.125716360553359 0.487423909991138 -2.25134335305349 3.22165376330082e-22 mirMAP -0.284743116153453 1.53758932404949e-05 NA NA NA hsa-miR-324-3p STEAP3 0.260348469146494 0.0506141704203545 -2.25134335305349 3.22165376330082e-22 MirTarget -0.439075296820338 8.37909093017194e-07 NA NA NA hsa-miR-330-5p STEAP3 0.442231058604099 0.0053308560843808 -2.25134335305349 3.22165376330082e-22 miRanda -0.261349210939533 0.000491490385998178 NA NA NA hsa-miR-452-3p STEAP3 1.76909792952222 2.98172274671408e-10 -2.25134335305349 3.22165376330082e-22 mirMAP -0.135225300015075 0.00112896926751927 NA NA NA hsa-miR-9-5p STEAP3 1.26407994492898 8.53156156439881e-05 -2.25134335305349 3.22165376330082e-22 MirTarget -0.131536126922079 0.000332697596459352 NA NA NA hsa-miR-16-1-3p THBS1 0.392360028038559 0.00112481918108198 -2.1667874641621 3.47909831637717e-17 MirTarget -0.349993916076067 0.00122857732566393 NA NA NA hsa-miR-17-5p THBS1 0.698861147045461 2.43854106455398e-05 -2.1667874641621 3.47909831637717e-17 miRNAWalker2_validate -0.222517583477087 0.00443051973072606 NA NA NA hsa-miR-19a-3p THBS1 1.01712596153672 2.57753201450625e-07 -2.1667874641621 3.47909831637717e-17 MirTarget;mirMAP -0.266530076936376 3.91252800717626e-05 NA NA NA hsa-miR-19b-3p THBS1 0.601279551368824 0.00016685097085251 -2.1667874641621 3.47909831637717e-17 MirTarget;mirMAP -0.258570375211438 0.00144531729895803 NA NA NA hsa-miR-20a-5p THBS1 0.854326758727003 5.04256669663285e-07 -2.1667874641621 3.47909831637717e-17 miRNAWalker2_validate -0.298190202548282 7.75927048516442e-05 NA NA NA hsa-miR-32-5p THBS1 0.0806126674664016 0.548984992497432 -2.1667874641621 3.47909831637717e-17 mirMAP -0.415642500380362 1.79938285205236e-05 NA NA NA hsa-miR-589-3p THBS1 1.16846704028095 5.88525574424201e-11 -2.1667874641621 3.47909831637717e-17 mirMAP -0.207376170994383 0.00450904846984429 NA NA NA hsa-miR-616-5p THBS1 0.150569904638223 0.402836556304524 -2.1667874641621 3.47909831637717e-17 mirMAP -0.380879712809061 1.2036408171943e-07 NA NA NA hsa-miR-618 THBS1 0.13539895350352 0.517145913114103 -2.1667874641621 3.47909831637717e-17 MirTarget;PITA -0.212645935824045 0.000996351251570771 NA NA NA hsa-miR-92a-3p THBS1 0.212391012518195 0.134292268089997 -2.1667874641621 3.47909831637717e-17 miRNAWalker2_validate -0.25627633804456 0.00550821085297264 NA NA NA hsa-miR-17-5p TNFRSF10B 0.698861147045461 2.43854106455398e-05 -0.612825437825455 1.81500500305993e-08 miRNAWalker2_validate -0.118682318607652 0.000225544481104091 NA NA NA hsa-miR-122-5p TP53 -1.23907441373955 4.42181244888642e-06 -0.163823087975333 0.268013799235932 miRNATAP -0.104401376732605 6.76735273123785e-05 23221562;22235305 Loss of miR-122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G1-modulated P53 activity.;Surprisingly miR-122 overexpression decreased the sensitivity to the chemotherapy-induced apoptosis via a signaling circuit involving the activation of Akt and inhibition of p53 ; liver cancer;lymphoma hsa-miR-25-3p TP53 0.633062563556379 3.68686354834328e-10 -0.163823087975333 0.268013799235932 miRNAWalker2_validate -0.198477612132015 0.00480268903724675 22431589 The results define miR-25 and -32 as positive regulators of p53 underscoring their role in tumorigenesis in glioblastoma tumorigenesis glioblastoma hsa-miR-26b-5p TP53I3 -1.10804856399744 4.14176110314282e-22 1.60533083447829 1.6335203778216e-17 miRNAWalker2_validate -0.218522423911536 0.00610780109634813 NA NA NA hsa-miR-10a-5p TP73 -1.47723516092033 7.27299669108673e-16 3.12737351940272 1.41162077669157e-23 mirMAP -0.650895757730988 2.49244233467107e-15 NA NA NA hsa-miR-127-5p TP73 -1.30084454872193 2.78105401208939e-07 3.12737351940272 1.41162077669157e-23 mirMAP -0.292062239826154 2.67364792194748e-06 NA NA NA hsa-miR-142-3p TP73 -1.4243101967815 1.92609481097765e-14 3.12737351940272 1.41162077669157e-23 miRanda -0.325422155570923 9.85928391154188e-05 NA NA NA hsa-miR-200b-3p TP73 -1.29335788099229 0.000268623234305059 3.12737351940272 1.41162077669157e-23 MirTarget;TargetScan -0.126731670549702 0.0049129791909471 23389960 Down regulation of miR 200b 3p by low p73 contributes to the androgen independence of prostate cancer cells; In this study we investigated the role that the interaction between miR-200b-3p and p73 plays in the proliferation of AIPC; Then we examined the effect of p73 and miR-200b-3p on the proliferation of AIPC and ADPC using CCK-8; Furthermore we investigated the regulation of miR-200b-3p by p73 prostate cancer hsa-miR-22-3p TP73 -0.628561621952407 1.33755558015523e-09 3.12737351940272 1.41162077669157e-23 mirMAP -0.808179945720649 1.06012353664804e-07 NA NA NA hsa-miR-24-3p TP73 -0.257825594021966 0.00689742600215061 3.12737351940272 1.41162077669157e-23 mirMAP -0.493125920104719 0.00383085887965063 NA NA NA hsa-miR-335-5p TP73 -1.61347836513558 1.62757083289711e-19 3.12737351940272 1.41162077669157e-23 mirMAP -0.494753534862885 5.84122531985338e-09 NA NA NA hsa-miR-429 TP73 -1.40262535827285 6.93982219171912e-05 3.12737351940272 1.41162077669157e-23 MirTarget;miRNATAP -0.161758102148855 0.000410585182804045 NA NA NA hsa-miR-542-3p TP73 -1.31351015149842 3.64642423288576e-22 3.12737351940272 1.41162077669157e-23 mirMAP -0.917477829359528 3.84907764521149e-17 NA NA NA hsa-miR-744-5p TSC2 -0.378868377317939 0.00125071229180016 0.454638169130336 1.34709422274577e-05 miRNAWalker2_validate -0.146650115106937 0.000784576818042153 NA NA NA hsa-miR-106b-5p ZMAT3 0.648901865047099 8.24870862047885e-09 -0.69014006285696 7.01619041120993e-05 mirMAP -0.371422232644537 5.40091407230955e-07 NA NA NA hsa-miR-130b-3p ZMAT3 0.687190305027546 0.000109068658562255 -0.69014006285696 7.01619041120993e-05 MirTarget -0.189133480259704 6.74227207588687e-05 NA NA NA hsa-miR-148b-3p ZMAT3 0.266862251671911 0.00185271302045935 -0.69014006285696 7.01619041120993e-05 MirTarget -0.409319286015021 4.26571564525349e-05 NA NA NA hsa-miR-148b-5p ZMAT3 0.295777213389605 0.0255749859535438 -0.69014006285696 7.01619041120993e-05 MirTarget -0.375231265680925 3.6206884202645e-09 NA NA NA hsa-miR-15a-5p ZMAT3 0.349521211668244 0.000770735998804736 -0.69014006285696 7.01619041120993e-05 MirTarget -0.318244124153394 0.000104023189915145 NA NA NA hsa-miR-17-5p ZMAT3 0.698861147045461 2.43854106455398e-05 -0.69014006285696 7.01619041120993e-05 mirMAP -0.298357924186218 2.9248147044618e-09 NA NA NA hsa-miR-186-5p ZMAT3 -0.060103391373687 0.535288717081205 -0.69014006285696 7.01619041120993e-05 MirTarget -0.230069177151057 0.0098256288493089 NA NA NA hsa-miR-193a-3p ZMAT3 -0.117675551589896 0.309391958208578 -0.69014006285696 7.01619041120993e-05 MirTarget -0.208714001196202 0.00491389321369164 NA NA NA hsa-miR-19a-3p ZMAT3 1.01712596153672 2.57753201450625e-07 -0.69014006285696 7.01619041120993e-05 MirTarget -0.274638008642666 4.41500672443795e-11 NA NA NA hsa-miR-19b-3p ZMAT3 0.601279551368824 0.00016685097085251 -0.69014006285696 7.01619041120993e-05 MirTarget -0.320735074486577 7.82081530608916e-10 NA NA NA hsa-miR-20a-5p ZMAT3 0.854326758727003 5.04256669663285e-07 -0.69014006285696 7.01619041120993e-05 mirMAP -0.279960410353906 9.5889879711568e-09 NA NA NA hsa-miR-20b-5p ZMAT3 0.457668023910379 0.0285865211936156 -0.69014006285696 7.01619041120993e-05 mirMAP -0.157885774368395 9.79108265057953e-05 NA NA NA hsa-miR-301a-3p ZMAT3 0.842910511640361 1.43446467417891e-06 -0.69014006285696 7.01619041120993e-05 MirTarget -0.293992950935408 5.22581016691463e-10 NA NA NA hsa-miR-32-3p ZMAT3 0.219109440121582 0.207217051619543 -0.69014006285696 7.01619041120993e-05 mirMAP -0.162528286402795 0.00134586898063536 NA NA NA hsa-miR-361-5p ZMAT3 0.233202959858696 0.00961572812255327 -0.69014006285696 7.01619041120993e-05 MirTarget;miRNATAP -0.30376272807027 0.00148295062886206 NA NA NA hsa-miR-362-3p ZMAT3 0.814007335895913 2.00108618836568e-09 -0.69014006285696 7.01619041120993e-05 MirTarget -0.252734114402692 3.94377042594587e-05 NA NA NA hsa-miR-362-5p ZMAT3 0.717921690799312 1.68392734504524e-05 -0.69014006285696 7.01619041120993e-05 mirMAP -0.24364168982292 1.22031003008872e-06 NA NA NA hsa-miR-421 ZMAT3 0.941856334694081 6.12167297054077e-08 -0.69014006285696 7.01619041120993e-05 mirMAP;miRNATAP -0.17261585769166 0.000355631292310814 NA NA NA hsa-miR-423-5p ZMAT3 0.698807775906485 1.02195196334612e-11 -0.69014006285696 7.01619041120993e-05 miRNATAP -0.40375372211695 5.80189151315166e-07 NA NA NA hsa-miR-425-5p ZMAT3 0.585974239943864 2.23063410931402e-05 -0.69014006285696 7.01619041120993e-05 MirTarget -0.339226610280544 2.04984703640177e-08 NA NA NA hsa-miR-454-3p ZMAT3 0.668749059573955 2.42670710515113e-06 -0.69014006285696 7.01619041120993e-05 MirTarget -0.327104315429301 2.61277115168845e-08 NA NA NA hsa-miR-501-5p ZMAT3 1.15231083477882 2.98906150004088e-10 -0.69014006285696 7.01619041120993e-05 MirTarget;mirMAP -0.187183487817675 3.77702438335579e-05 NA NA NA hsa-miR-590-5p ZMAT3 -0.103355559980404 0.310027295317092 -0.69014006285696 7.01619041120993e-05 miRanda -0.39348063958649 2.75527029509159e-06 NA NA NA hsa-miR-616-5p ZMAT3 0.150569904638223 0.402836556304524 -0.69014006285696 7.01619041120993e-05 mirMAP -0.216766048377957 4.38996414394084e-06 NA NA NA hsa-miR-660-5p ZMAT3 0.989755850734071 5.69930767464937e-14 -0.69014006285696 7.01619041120993e-05 mirMAP -0.379437182311435 8.54151840643677e-10 NA NA NA hsa-miR-664a-3p ZMAT3 0.485807855553031 0.000732553249798598 -0.69014006285696 7.01619041120993e-05 mirMAP -0.164195707524401 0.00549371371473217 NA NA NA hsa-miR-671-5p ZMAT3 0.843645373345787 3.92794196273313e-08 -0.69014006285696 7.01619041120993e-05 MirTarget -0.182198202957686 0.000866018116035851 NA NA NA hsa-miR-93-5p ZMAT3 1.39518349670953 2.84167728726712e-25 -0.69014006285696 7.01619041120993e-05 mirMAP -0.439163271969714 4.39719497452434e-14 NA NA NA hsa-miR-942-5p ZMAT3 0.346451845105263 0.0283255399972342 -0.69014006285696 7.01619041120993e-05 MirTarget -0.155132831202 0.00405148420000063 NA NA NA