miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-28-5p AAAS -0.14267073663385 0.0497619246260571 0.333961696450526 1.76247956290803e-10 miRNAWalker2_validate -0.192144123006222 8.0625876745442e-15 NA NA NA hsa-miR-28-5p AAGAB -0.14267073663385 0.0497619246260571 0.814198545635563 5.08425148014984e-36 miRanda -0.261414067779177 1.78986406092867e-16 NA NA NA hsa-miR-28-5p AATK -0.14267073663385 0.0497619246260571 0.453430702519743 0.00408123503396711 miRanda -0.27417826152726 0.000266548654540702 NA NA NA hsa-miR-28-5p ABCB9 -0.14267073663385 0.0497619246260571 0.947933279325208 1.1543853299555e-17 miRanda -0.262888980111223 9.28660818341309e-07 NA NA NA hsa-miR-28-5p ABCC5 -0.14267073663385 0.0497619246260571 0.797302989981293 1.93030507748535e-11 miRanda -0.158910926194168 0.00553032764471306 NA NA NA hsa-miR-28-5p ABHD1 -0.14267073663385 0.0497619246260571 -0.979242702067246 7.11422819612358e-10 miRanda -0.304354305354412 6.60803787379825e-05 NA NA NA hsa-miR-28-5p ABLIM3 -0.14267073663385 0.0497619246260571 -1.87900809021962 1.5479100001313e-23 miRanda -0.636062725407327 2.75091626853684e-12 NA NA NA hsa-miR-28-5p ACACA -0.14267073663385 0.0497619246260571 -0.221648651129382 0.021619749795881 miRanda -0.161588815074034 0.00043088920351934 NA NA NA hsa-miR-28-5p ACAD8 -0.14267073663385 0.0497619246260571 -0.35954398205226 2.03716487332537e-07 miRanda -0.124555556230843 0.000165960983426681 NA NA NA hsa-miR-28-5p ACAT1 -0.14267073663385 0.0497619246260571 -0.751385545284676 2.05460285005042e-17 miRanda -0.156118622908354 0.000274432130308958 NA NA NA hsa-miR-28-5p ACOX3 -0.14267073663385 0.0497619246260571 0.203718310604422 0.0232698998426671 miRanda -0.184836000309236 1.4443916193675e-05 NA NA NA hsa-miR-28-5p ACSF2 -0.14267073663385 0.0497619246260571 -0.25646487247878 0.0548866843292958 miRanda -0.311846743736682 8.46857904574377e-07 NA NA NA hsa-miR-28-5p ADAMTS13 -0.14267073663385 0.0497619246260571 0.49002440332112 0.000583985090350807 miRanda -0.252476874446622 0.000201582106068662 NA NA NA hsa-miR-28-5p ADCK1 -0.14267073663385 0.0497619246260571 0.187517081387471 0.00450919989695981 miRanda -0.120939647200665 0.000115520676281999 NA NA NA hsa-miR-28-5p ADCY1 -0.14267073663385 0.0497619246260571 -0.0486801025784516 0.841490665662841 miRanda -0.592080609407235 2.7807221777423e-07 NA NA NA hsa-miR-28-5p ADCY6 -0.14267073663385 0.0497619246260571 -0.631200513666625 6.14065292828624e-14 miRanda -0.322887202501352 6.58653319288642e-16 NA NA NA hsa-miR-28-5p AFF1 -0.14267073663385 0.0497619246260571 -0.822613403095266 3.54527732954134e-21 miRanda -0.181485420169248 1.84515565349079e-05 NA NA NA hsa-miR-28-5p AFF4 -0.14267073663385 0.0497619246260571 -0.281510843525455 0.00153080576834839 miRanda -0.198317096353644 2.59227723832472e-06 NA NA NA hsa-miR-28-5p AFTPH -0.14267073663385 0.0497619246260571 0.310042514383331 6.63540668673408e-07 miRanda -0.100572748665942 0.000737307674554123 NA NA NA hsa-miR-28-5p AGAP11 -0.14267073663385 0.0497619246260571 -2.71964004520601 8.30765328552898e-62 miRanda -0.311912698282939 0.000215531155380505 NA NA NA hsa-miR-28-5p AGBL4 -0.14267073663385 0.0497619246260571 -0.142971148431008 0.45128658353738 miRanda -0.235687807620684 0.00911628251703385 NA NA NA hsa-let-7e-5p AGMAT 0.261471269338985 0.00191077095550257 1.48756865195736 3.00482085953982e-31 miRNAWalker2_validate -0.109174829152805 0.044920987444433 NA NA NA hsa-miR-28-5p AHNAK -0.14267073663385 0.0497619246260571 -1.76535987346934 5.20116033627817e-56 miRanda -0.267450672419509 2.68436282191842e-06 NA NA NA hsa-miR-28-5p AKR7A2 -0.14267073663385 0.0497619246260571 0.343255312465302 8.62257546469888e-06 miRanda -0.23936896990541 5.47058062758386e-11 NA NA NA hsa-miR-143-3p AKT1 -1.23248181631499 4.74160976678504e-28 0.675644771423915 4.31393881321574e-23 miRNAWalker2_validate -0.113351907486334 3.74647210660112e-08 NA NA NA hsa-miR-28-5p ALDH3A2 -0.14267073663385 0.0497619246260571 -0.860769652123822 9.84587277412076e-16 miRanda -0.292717815903004 1.40563132631418e-08 NA NA NA hsa-miR-28-5p AMIGO2 -0.14267073663385 0.0497619246260571 -1.57919489364877 1.03555350832452e-17 miRanda -0.238335214108372 0.00783964240570731 NA NA NA hsa-miR-28-5p AMPH -0.14267073663385 0.0497619246260571 -0.229137854504136 0.257667598863092 miRanda -0.379789131994063 7.84571508819915e-05 NA NA NA hsa-miR-28-5p AMZ1 -0.14267073663385 0.0497619246260571 2.49247827940287 4.85570356046494e-25 miRanda -0.449900404852048 0.000141115554584685 NA NA NA hsa-miR-28-5p ANGEL1 -0.14267073663385 0.0497619246260571 -0.244973705180581 5.45027775560672e-05 miRanda -0.140734378364615 1.04645383987188e-06 NA NA NA hsa-miR-28-5p ANKS1B -0.14267073663385 0.0497619246260571 0.195574172772792 0.219403504513109 miRanda -0.42370630515817 1.82751876578841e-08 NA NA NA hsa-miR-28-5p ANKS3 -0.14267073663385 0.0497619246260571 0.722561297123601 2.02940894883196e-12 miRanda -0.161675758886296 0.00110175526475344 NA NA NA hsa-miR-28-5p ANXA9 -0.14267073663385 0.0497619246260571 1.77703853413407 4.65169713363917e-15 miRanda -1.04785141788144 1.34518763922765e-22 NA NA NA hsa-miR-28-5p AP2A2 -0.14267073663385 0.0497619246260571 0.0276176473817644 0.559431685651471 miRanda -0.117522775082671 1.3499752433353e-07 NA NA NA hsa-miR-28-5p AP2B1 -0.14267073663385 0.0497619246260571 0.256868055359782 0.00182779940285162 miRanda -0.218773775664612 2.05664703731146e-08 NA NA NA hsa-miR-145-5p APH1A -2.62703571936687 7.64402900188551e-88 0.880951296770373 4.04327073394667e-49 miRNAWalker2_validate -0.175822049334125 1.47914936534563e-35 NA NA NA hsa-miR-28-5p ARAF -0.14267073663385 0.0497619246260571 0.235957457636243 1.23308070405748e-05 miRanda -0.105409266420207 4.05384033466484e-05 NA NA NA hsa-miR-132-3p ARHGAP32 -0.0365213889238607 0.668598301088667 0.428981349890582 9.04481455196716e-05 miRNAWalker2_validate;miRTarBase -0.220067441144533 6.75438325916112e-07 NA NA NA hsa-miR-28-5p ARHGAP32 -0.14267073663385 0.0497619246260571 0.428981349890582 9.04481455196716e-05 miRanda -0.357813655513258 4.69862929924358e-12 NA NA NA hsa-miR-28-5p ARHGAP8 -0.14267073663385 0.0497619246260571 1.29054868351649 1.27181467936902e-24 miRanda -0.123720338679521 0.0455894799146789 NA NA NA hsa-miR-28-5p ARHGEF16 -0.14267073663385 0.0497619246260571 1.35329840541901 1.42152949394676e-31 miRanda -0.311713075778674 4.38350949037839e-08 NA NA NA hsa-miR-28-5p ARL17A -0.14267073663385 0.0497619246260571 -0.076616628706041 0.376773217715529 miRanda -0.116838808707706 0.00459030737152711 NA NA NA hsa-miR-28-5p ARL6IP4 -0.14267073663385 0.0497619246260571 0.502725444320326 1.62774393703499e-06 miRanda -0.187164035456642 0.000188216832903953 NA NA NA hsa-miR-28-5p ARMC5 -0.14267073663385 0.0497619246260571 0.672611492140303 1.59874426366769e-13 miRanda -0.281851896737958 1.02406182812613e-10 NA NA NA hsa-miR-28-5p ARMC7 -0.14267073663385 0.0497619246260571 0.786794624029855 9.0622398933339e-30 miRanda -0.125003459010202 0.000256153442255009 NA NA NA hsa-miR-28-5p ARMC9 -0.14267073663385 0.0497619246260571 1.05145638032088 4.80180385675785e-40 miRanda -0.181814932736477 4.29206202175496e-06 NA NA NA hsa-miR-28-5p ASB8 -0.14267073663385 0.0497619246260571 -0.209242682677155 0.000120963170245691 miRanda -0.187314696249896 2.48215666088341e-13 NA NA NA hsa-miR-132-3p ATP6V0E1 -0.0365213889238607 0.668598301088667 0.38487806855278 3.42056300307015e-10 miRNAWalker2_validate -0.100144558249853 6.06677528546474e-05 NA NA NA hsa-miR-28-5p ATPAF1 -0.14267073663385 0.0497619246260571 -0.162517192737524 0.00531751879757489 miRanda -0.121883670377972 1.03894860881348e-05 NA NA NA hsa-miR-28-5p ATRNL1 -0.14267073663385 0.0497619246260571 -0.735629381854826 0.0208064652732134 miRanda -0.528569962012974 0.000487200551841339 NA NA NA hsa-miR-28-5p B3GALT4 -0.14267073663385 0.0497619246260571 0.463435175029749 5.58445598817781e-06 miRanda -0.144809845762043 0.00301366337812068 NA NA NA hsa-miR-28-5p BAG1 -0.14267073663385 0.0497619246260571 0.415270048181819 1.74825976512713e-05 miRanda -0.307899469150691 1.6573691753667e-11 NA NA NA hsa-miR-28-5p BBS1 -0.14267073663385 0.0497619246260571 -0.0933887460229856 0.273588503662833 miRanda -0.28940058756879 4.91592702721265e-13 NA NA NA hsa-miR-28-5p BCAM -0.14267073663385 0.0497619246260571 0.514670465767451 0.00080836907714589 miRanda -0.64381725099197 3.09853416103942e-19 NA NA NA hsa-miR-28-5p BHLHE40 -0.14267073663385 0.0497619246260571 0.408671974638585 0.000468982708659732 miRanda -0.470045059510164 8.62962982184735e-18 NA NA NA hsa-let-7e-5p BMP2K 0.261471269338985 0.00191077095550257 -0.606665941487059 3.28263902417702e-08 miRNAWalker2_validate -0.110871945655889 0.0143432932517263 NA NA NA hsa-miR-28-5p BTRC -0.14267073663385 0.0497619246260571 0.120536048906525 0.159286798668058 miRanda -0.245382923565365 1.23962341723426e-09 NA NA NA hsa-miR-28-5p CACNG4 -0.14267073663385 0.0497619246260571 2.21159891256783 3.76360584804154e-13 miRanda -0.632449765922558 1.63314781237986e-05 NA NA NA hsa-miR-28-5p CALCOCO1 -0.14267073663385 0.0497619246260571 -1.08289042748634 4.79337264485307e-51 miRanda -0.143823356702789 8.02577597264752e-05 NA NA NA hsa-miR-28-5p CALCOCO2 -0.14267073663385 0.0497619246260571 -0.438580916376234 2.42826269547857e-08 miRanda -0.198957671269254 1.11358465771385e-07 NA NA NA hsa-miR-28-5p CANT1 -0.14267073663385 0.0497619246260571 1.19121908849889 2.01312112605851e-40 miRanda -0.31350040187146 1.39014322710422e-12 NA NA NA hsa-miR-28-5p CASC2 -0.14267073663385 0.0497619246260571 -0.0591451463253274 0.56145154040616 miRanda -0.214324545590939 9.02943345410797e-06 NA NA NA hsa-miR-132-3p CASP7 -0.0365213889238607 0.668598301088667 0.0960684078333616 0.182578616272612 miRNAWalker2_validate -0.107439780267955 0.000220260395052539 NA NA NA hsa-miR-28-5p CCDC113 -0.14267073663385 0.0497619246260571 0.149223101788104 0.136932129872727 miRanda -0.148195425613187 0.00190132838114588 NA NA NA hsa-miR-28-5p CCDC114 -0.14267073663385 0.0497619246260571 1.16840637134549 3.00376939451887e-11 miRanda -0.296693787504866 0.000456912265203965 NA NA NA hsa-miR-28-5p CCDC85A -0.14267073663385 0.0497619246260571 -2.63514416883509 8.247493889982e-55 miRanda -0.198190978658007 0.0217102405686434 NA NA NA hsa-miR-28-5p CCDC87 -0.14267073663385 0.0497619246260571 1.38078268229956 9.83612478312899e-22 miRanda -0.498038418591484 8.14796611713182e-13 NA NA NA hsa-miR-28-5p CCNDBP1 -0.14267073663385 0.0497619246260571 -0.725809699931222 3.00753297514094e-26 miRanda -0.172490460917306 2.48035110164691e-07 NA NA NA hsa-miR-28-5p CCNG2 -0.14267073663385 0.0497619246260571 -0.149650768150032 0.140842306998986 miRanda -0.202065115294172 2.7892462854838e-05 NA NA NA hsa-miR-132-3p CD164 -0.0365213889238607 0.668598301088667 -0.285138667648189 0.000280402525232159 miRNAWalker2_validate -0.188310804381255 2.4797384072156e-09 NA NA NA hsa-miR-28-5p CD2BP2 -0.14267073663385 0.0497619246260571 0.7295100071912 3.69314996005442e-25 miRanda -0.242214534749734 1.19789821252281e-12 NA NA NA hsa-miR-132-3p CD55 -0.0365213889238607 0.668598301088667 -0.357125668177814 0.00101673697013703 miRNAWalker2_validate -0.118990266693861 0.00697265157564722 NA NA NA hsa-miR-28-5p CDH18 -0.14267073663385 0.0497619246260571 -0.0522684838785148 0.841570242720998 miRanda -0.338750908305708 0.00650983425165781 NA NA NA hsa-miR-28-5p CDH7 -0.14267073663385 0.0497619246260571 1.31238154715945 1.99846237004699e-05 miRanda -0.556872122656275 0.000151564494061746 NA NA NA hsa-miR-28-5p CDK17 -0.14267073663385 0.0497619246260571 -0.124778185173291 0.119991532413407 miRanda -0.2679902930505 1.16916140085302e-12 NA NA NA hsa-miR-145-5p CDK4 -2.62703571936687 7.64402900188551e-88 0.597969315491349 4.23701383421285e-22 miRNAWalker2_validate;miRTarBase -0.134364969525933 9.65405723067241e-21 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-28-5p CDK5 -0.14267073663385 0.0497619246260571 1.49472759404501 6.61129562064286e-69 miRanda -0.232036778076655 1.32632021154552e-07 NA NA NA hsa-miR-28-5p CDKN1A -0.14267073663385 0.0497619246260571 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;miRanda -0.294350864895331 4.29596264827348e-08 NA NA NA hsa-miR-28-5p CDON -0.14267073663385 0.0497619246260571 -0.991164686357028 8.70119211480039e-13 miRanda -0.218069047933018 0.00111790580801383 NA NA NA hsa-miR-28-5p CEACAM6 -0.14267073663385 0.0497619246260571 3.14788813465337 6.1411751537789e-15 miRanda -1.28041560860565 3.67270918208962e-11 NA NA NA hsa-miR-28-5p CEACAM7 -0.14267073663385 0.0497619246260571 1.13710462023162 6.55115186081843e-05 miRanda -1.0999950998306 2.08904069363437e-16 NA NA NA hsa-let-7e-5p CELF2 0.261471269338985 0.00191077095550257 -2.12375294826078 1.08191631254129e-58 miRNAWalker2_validate -0.211640448758577 0.000251932180152815 NA NA NA hsa-miR-28-5p CHAD -0.14267073663385 0.0497619246260571 -0.0030842411669636 0.993051648329624 miRanda -0.898299919129268 8.28846753994342e-08 NA NA NA hsa-miR-28-5p CHD3 -0.14267073663385 0.0497619246260571 0.23471658817734 0.000437572538712428 miRanda -0.14699452182334 3.51869616627388e-06 NA NA NA hsa-miR-28-5p CHDH -0.14267073663385 0.0497619246260571 0.0931083294714723 0.508817983929441 miRanda -0.296667633251818 9.04941339500822e-06 NA NA NA hsa-miR-28-5p CLASP2 -0.14267073663385 0.0497619246260571 -0.480530283040537 1.81219003638834e-11 miRanda -0.162061212588336 2.27538557457813e-06 NA NA NA hsa-miR-28-5p CNKSR1 -0.14267073663385 0.0497619246260571 1.44823291536679 2.12293533329982e-27 miRanda -0.136690806427665 0.0378724954718156 NA NA NA hsa-miR-28-5p COL3A1 -0.14267073663385 0.0497619246260571 1.50470077229731 1.9069386527602e-18 miRanda -0.217177944731906 0.00939470887863092 NA NA NA hsa-miR-28-5p COL6A3 -0.14267073663385 0.0497619246260571 0.634653480531805 2.31261246968383e-05 miRanda -0.142973027863578 0.0465166725475489 NA NA NA hsa-miR-28-5p COMMD3 -0.14267073663385 0.0497619246260571 0.675388089068306 6.93374609568002e-19 miRanda -0.134682275248439 0.000267418960162858 NA NA NA hsa-miR-28-5p COPG2 -0.14267073663385 0.0497619246260571 0.248679199189896 0.000341940906197054 miRanda -0.181006340148697 3.74563245844575e-08 NA NA NA hsa-miR-28-5p CPD -0.14267073663385 0.0497619246260571 -0.241449324374273 0.0207834781120828 miRanda -0.259823794381588 1.4995780268069e-07 NA NA NA hsa-miR-28-5p CRADD -0.14267073663385 0.0497619246260571 -0.251683647119455 0.000231223686713744 miRanda -0.235221289528442 2.61428453267022e-13 NA NA NA hsa-miR-28-5p CREBL2 -0.14267073663385 0.0497619246260571 -0.828924810318552 7.72831390224407e-35 miRanda -0.118954483680499 0.000365495180109717 NA NA NA hsa-miR-28-5p CROT -0.14267073663385 0.0497619246260571 0.037293899986957 0.777067375226551 miRanda -0.437819717941343 1.51683811218234e-12 NA NA NA hsa-miR-28-5p CRY2 -0.14267073663385 0.0497619246260571 -1.19975346543936 7.77211531373058e-53 miRanda -0.215089887065039 5.72190749767741e-08 NA NA NA hsa-miR-132-3p CST9L -0.0365213889238607 0.668598301088667 0.900727643330873 0.00141190357480774 miRNAWalker2_validate -0.256074331330126 0.0254955857615731 NA NA NA hsa-miR-28-5p CYTH2 -0.14267073663385 0.0497619246260571 0.702575167222823 2.04335535926724e-21 miRanda -0.196402252030897 4.26017752822191e-08 NA NA NA hsa-miR-28-5p DALRD3 -0.14267073663385 0.0497619246260571 0.592133186684577 7.32758102420887e-12 miRanda -0.448285991408448 8.89585675977247e-29 NA NA NA hsa-miR-28-5p DAZAP2 -0.14267073663385 0.0497619246260571 0.0462419874292657 0.329536160227049 miRanda -0.167753753611657 3.68313522146614e-14 NA NA NA hsa-miR-28-5p DDX60 -0.14267073663385 0.0497619246260571 0.258448146031201 0.049511423209945 miRanda -0.124121210231133 0.0479397801929396 NA NA NA hsa-miR-28-5p DHRS2 -0.14267073663385 0.0497619246260571 1.07976211540207 0.0180138769274907 miRanda -1.42153982969568 4.1358623924697e-11 NA NA NA hsa-miR-28-5p DHRS4 -0.14267073663385 0.0497619246260571 0.0781364876424782 0.360324485265141 miRanda -0.252257675076986 3.65800347648958e-10 NA NA NA hsa-miR-28-5p DHRS4L1 -0.14267073663385 0.0497619246260571 -0.0991231860873832 0.44302544820008 miRanda -0.346024158096003 1.45044398203217e-08 NA NA NA hsa-miR-28-5p DHRS4L2 -0.14267073663385 0.0497619246260571 -0.0131580653571994 0.885661344969612 miRanda -0.240307182431194 2.6902141983072e-08 NA NA NA hsa-miR-28-5p DHRS7B -0.14267073663385 0.0497619246260571 -0.227646059774749 0.0024852530623363 miRanda -0.196022437875585 3.73712774456641e-08 NA NA NA hsa-miR-28-5p DHX29 -0.14267073663385 0.0497619246260571 0.0734426982923981 0.254121869868459 miRanda -0.227299174298341 4.81576551681987e-14 NA NA NA hsa-miR-28-5p DHX58 -0.14267073663385 0.0497619246260571 0.568647882524633 8.82962162841857e-09 miRanda -0.196598808324417 3.29420558267845e-05 NA NA NA hsa-miR-28-5p DNAJA4 -0.14267073663385 0.0497619246260571 0.778974960199304 3.35701271270575e-11 miRanda -0.367555686579116 5.42936568736381e-11 NA NA NA hsa-miR-28-5p DNAJB14 -0.14267073663385 0.0497619246260571 -0.389536819121818 1.15940870921076e-05 miRanda -0.130292186583654 0.00214996577325443 NA NA NA hsa-miR-28-5p DNAJC12 -0.14267073663385 0.0497619246260571 1.43850602777815 2.34358351695651e-08 miRanda -1.08463819946357 3.47364266841502e-19 NA NA NA hsa-miR-28-5p DNMBP -0.14267073663385 0.0497619246260571 -0.666936518001947 1.27257555078449e-08 miRanda -0.284585338956239 3.74226293978231e-07 NA NA NA hsa-miR-143-3p DNMT3A -1.23248181631499 4.74160976678504e-28 0.954713787765479 1.01650948201553e-39 miRNAWalker2_validate;miRTarBase -0.134719476301317 1.50584906913356e-09 24218337;19638978 Ectopic expression of miR-143 inhibited proliferation and soft agar colony formation of breast cancer cells and also downregulated DNA methyltransferase 3A DNMT3A expression on both mRNA and protein levels; DNMT3A was demonstrated to be a direct target of miR-143 by luciferase reporter assay; Furthermore miR-143 expression was observed to be inversely correlated with DNMT3A mRNA and protein expression in breast cancer tissues; Our findings suggest that miR-143 regulates DNMT3A in breast cancer cells;Using in silico predictions DNA methyltranferase 3A DNMT3A was defined as a potential target of miR-143; Restoration of the miR-143 expression in colon cell lines decreased tumour cell growth and soft-agar colony formation and downregulated the DNMT3A expression in both mRNA and protein levels; DNMT3A was shown to be a direct target of miR-143 by luciferase reporter assay; Furthermore the miR-143 expression was observed to be inversely correlated with DNMT3A mRNA and protein expression in CRC tissues; Our findings suggest that miR-143 regulates DNMT3A in CRC ; breast cancer;colorectal cancer hsa-miR-28-5p DPH1 -0.14267073663385 0.0497619246260571 -0.0275786670934695 0.663368667669771 miRanda -0.13551892924721 6.16360587687299e-06 NA NA NA hsa-miR-28-5p DPP3 -0.14267073663385 0.0497619246260571 1.58410857657904 3.35818668883493e-77 miRanda -0.185549722751376 3.15204874454426e-05 NA NA NA hsa-miR-28-5p DQX1 -0.14267073663385 0.0497619246260571 3.64196683187679 4.5119403521697e-36 miRanda -0.792329778275761 3.56456901527464e-08 NA NA NA hsa-miR-28-5p DTWD2 -0.14267073663385 0.0497619246260571 -0.261106719372882 0.00652226445516583 miRanda -0.319410509963769 1.55805399694254e-12 NA NA NA hsa-miR-28-5p DYNC2LI1 -0.14267073663385 0.0497619246260571 -0.00277231382927301 0.963653940981411 miRanda -0.131841275234267 4.59676931998792e-06 NA NA NA hsa-miR-28-5p DYNLL2 -0.14267073663385 0.0497619246260571 0.0640586851492353 0.385985405743691 miRanda -0.138480243677895 7.74924044116613e-05 NA NA NA hsa-miR-28-5p DYNLRB2 -0.14267073663385 0.0497619246260571 0.0266923189330223 0.906740352238072 miRanda -0.964471145480037 9.91972082004647e-20 NA NA NA hsa-miR-205-5p E2F1 -1.70057043266505 1.20976599540743e-09 2.60062333464716 6.00545189391833e-64 miRNAWalker2_validate;miRTarBase -0.154229027607252 9.82898858548395e-15 22578566;21454583 Re-expression of miR-205 where absent strongly reduces cell proliferation cell cycle progression and clonogenic potential in vitro and inhibits tumor growth in vivo and this tumor suppressor activity is at least partially exerted through targeting of E2F1 master regulator of cell cycle progression and LAMC1 component of extracellular matrix involved in cell adhesion proliferation and migration;The expression levels of E2F1 and E2F5 were correlated inversely with that of miR-205 in melanoma cell lines; miR-205 significantly suppressed the luciferase activity of reporter plasmids containing the 3'-UTR sequences complementary to either E2F1 or E2F5; Overexpression of miR-205 in melanoma cells reduced E2F1 and E2F5 protein levels progression; breast cancer;melanoma hsa-miR-28-5p EDA2R -0.14267073663385 0.0497619246260571 -1.34255111275385 3.98620322593049e-21 miRanda -0.231407177745149 0.000862123744152227 NA NA NA hsa-miR-200c-3p EDNRA 2.07038651806183 6.84613386286599e-51 -0.14153486543887 0.241849668080954 miRNAWalker2_validate -0.2381009935223 7.07311760507522e-18 NA NA NA hsa-miR-28-5p EGLN3 -0.14267073663385 0.0497619246260571 1.25406110119931 1.58374008569191e-13 miRanda -0.214502668135583 0.00907143930411108 NA NA NA hsa-miR-28-5p EIF2AK1 -0.14267073663385 0.0497619246260571 0.859351234809848 8.38838356282298e-53 miRanda -0.143546463072448 4.26354140335158e-07 NA NA NA hsa-miR-28-5p EIF4E3 -0.14267073663385 0.0497619246260571 -1.14472452087938 6.75636040536301e-35 miRanda -0.204829970524929 8.50373422668106e-06 NA NA NA hsa-miR-28-5p ELMOD3 -0.14267073663385 0.0497619246260571 -1.18321142026853 1.42445980731353e-54 miRanda -0.129213859015614 0.000848520939567749 NA NA NA hsa-miR-28-5p ENOSF1 -0.14267073663385 0.0497619246260571 -0.0494377033754967 0.530975780572447 miRanda -0.116679544081481 0.00185633881646488 NA NA NA hsa-miR-28-5p ENPP5 -0.14267073663385 0.0497619246260571 0.730179563987445 0.000250569116717678 miRanda -0.430134462533778 5.87774849114148e-06 NA NA NA hsa-miR-28-5p EPB41L4A -0.14267073663385 0.0497619246260571 -0.674310150249758 9.72635451687197e-08 miRanda -0.387849374944374 1.07747570210006e-10 NA NA NA hsa-miR-28-5p EPM2AIP1 -0.14267073663385 0.0497619246260571 -0.437165458818003 3.86366376911147e-08 miRanda -0.144548878665864 0.000146275825401192 NA NA NA hsa-miR-28-5p EPN1 -0.14267073663385 0.0497619246260571 0.758288535610807 2.09677654098872e-20 miRanda -0.122761755854016 0.00209899863394848 NA NA NA hsa-miR-28-5p EPN2 -0.14267073663385 0.0497619246260571 -0.0264754384448693 0.695378898008471 miRanda -0.161848837849795 4.07182269870998e-07 NA NA NA hsa-miR-28-5p ERAL1 -0.14267073663385 0.0497619246260571 0.598584957474529 1.72513784158031e-17 miRanda -0.125046069253116 0.000243068164108692 NA NA NA hsa-miR-28-5p ERGIC1 -0.14267073663385 0.0497619246260571 0.646215620956665 8.40261132551627e-14 miRanda -0.421087236029744 3.74633309969529e-25 NA NA NA hsa-miR-28-5p ERI2 -0.14267073663385 0.0497619246260571 0.367887024257485 6.00654283242427e-06 miRanda -0.26523497387734 5.06048254756163e-12 NA NA NA hsa-miR-28-5p ERLIN2 -0.14267073663385 0.0497619246260571 0.0041901531172277 0.969544348188735 miRanda -0.253389185184216 1.08006189075056e-06 NA NA NA hsa-miR-200c-3p ERRFI1 2.07038651806183 6.84613386286599e-51 -1.06077137481255 1.16711058066714e-20 miRNAWalker2_validate;miRTarBase -0.138484555642197 3.0695306989007e-07 NA NA NA hsa-miR-200b-3p ETS1 2.11744072703209 1.66710572104191e-43 -0.979239394867202 3.4732433286646e-20 miRNAWalker2_validate;miRTarBase -0.246496070854348 2.76376768290262e-28 NA NA NA hsa-miR-28-5p EXOC6 -0.14267073663385 0.0497619246260571 0.307205555860763 0.000882348548359484 miRanda -0.330426623232935 2.76721875618044e-14 NA NA NA hsa-miR-132-3p EYA4 -0.0365213889238607 0.668598301088667 -1.70735688835573 6.25677946773534e-11 miRNAWalker2_validate -0.23586747210908 0.0276804462942293 NA NA NA hsa-miR-145-5p F11R -2.62703571936687 7.64402900188551e-88 0.784299320718817 2.24787898258571e-28 miRNAWalker2_validate -0.117356053758665 3.24546654311589e-12 20818426;26374689 Our data identify JAM-A and fascin as novel targets of miR-145 firmly establishing a role for miR-145 in modulating breast cancer cell motility;MicroRNA screening predicted that microRNA-145 miR-145 would bind to JAM-A; Using patient-derived glioblastoma CSCs we confirmed that JAM-A is suppressed by miR-145 motility; breast cancer;glioblastoma hsa-miR-28-5p F2RL2 -0.14267073663385 0.0497619246260571 1.83288694519631 1.27552904921777e-14 miRanda -0.456824867145525 7.02447930509342e-05 NA NA NA hsa-miR-28-5p FAHD1 -0.14267073663385 0.0497619246260571 0.778155825824243 6.70403995205136e-28 miRanda -0.253441421631961 2.04651536156701e-13 NA NA NA hsa-miR-28-5p FAM110B -0.14267073663385 0.0497619246260571 0.446891190279147 0.00220027405081027 miRanda -0.438812554775886 2.00385789711424e-10 NA NA NA hsa-miR-28-5p FAM134C -0.14267073663385 0.0497619246260571 0.222345364858138 0.000232535871253503 miRanda -0.179678608103432 2.9924102541235e-10 NA NA NA hsa-miR-28-5p FAM135B -0.14267073663385 0.0497619246260571 0.297929137431374 0.2926521551134 miRanda -0.639664644933673 1.88481695577419e-06 NA NA NA hsa-miR-28-5p FAM151B -0.14267073663385 0.0497619246260571 0.353772763587107 0.000222374712049446 miRanda -0.356303249159723 2.48752766078358e-15 NA NA NA hsa-miR-28-5p FAM160A2 -0.14267073663385 0.0497619246260571 -0.147263090451403 0.00579602030005972 miRanda -0.120301491046325 1.91930813386215e-06 NA NA NA hsa-miR-28-5p FAM169A -0.14267073663385 0.0497619246260571 0.000732433647964825 0.996131193444778 miRanda -0.173595504883953 0.0158016800253962 NA NA NA hsa-miR-132-3p FAM199X -0.0365213889238607 0.668598301088667 0.253187015339215 0.000942799939057211 miRNAWalker2_validate -0.188261246093114 9.3797281497346e-10 NA NA NA hsa-miR-28-5p FANK1 -0.14267073663385 0.0497619246260571 0.727752467493866 5.08127758503224e-06 miRanda -0.326148845787742 1.8492440727162e-05 NA NA NA hsa-miR-200c-3p FBLN5 2.07038651806183 6.84613386286599e-51 -2.08643289665163 1.31128583188143e-54 miRNAWalker2_validate;miRTarBase -0.431582532539337 7.04635991309229e-42 NA NA NA hsa-miR-132-3p FBXO28 -0.0365213889238607 0.668598301088667 0.207643878912418 0.00290746501599163 miRNAWalker2_validate -0.171045732332445 1.00024523781095e-09 NA NA NA hsa-miR-28-5p FBXW4 -0.14267073663385 0.0497619246260571 -0.0900849463685356 0.206348671758354 miRanda -0.152099944153854 6.63763082332348e-06 NA NA NA hsa-miR-28-5p FCGRT -0.14267073663385 0.0497619246260571 -0.474452179789248 1.11132066883725e-05 miRanda -0.234186660196465 5.33644165622309e-06 NA NA NA hsa-miR-28-5p FCHO2 -0.14267073663385 0.0497619246260571 -0.606061420659775 1.08535609457921e-15 miRanda -0.141100901822869 0.000112527994366655 NA NA NA hsa-miR-28-5p FEM1C -0.14267073663385 0.0497619246260571 -0.260293397561805 0.000498044801322955 miRanda -0.176460573027483 6.55806438951583e-07 NA NA NA hsa-miR-200c-3p FHOD1 2.07038651806183 6.84613386286599e-51 0.172964188564771 0.0354183224027716 miRNAWalker2_validate -0.113582055183826 2.29895309973769e-09 22144583 MicroRNA 200c represses migration and invasion of breast cancer cells by targeting actin regulatory proteins FHOD1 and PPM1F; We identified FHOD1 and PPM1F direct regulators of the actin cytoskeleton as novel targets of miR-200c; Remarkably expression levels of FHOD1 and PPM1F were inversely correlated with the level of miR-200c in breast cancer cell lines breast cancer patient samples and 58 cancer cell lines of various origins breast cancer hsa-miR-28-5p FKTN -0.14267073663385 0.0497619246260571 0.0913291179055893 0.215754519232549 miRanda -0.144817438914449 3.46968527462469e-05 NA NA NA hsa-miR-200c-3p FLNA 2.07038651806183 6.84613386286599e-51 -0.588648086634478 1.12488656429227e-08 miRNAWalker2_validate -0.200293197507804 3.85868552345297e-17 NA NA NA hsa-miR-200b-3p FLT1 2.11744072703209 1.66710572104191e-43 -0.976682151789582 4.79604479745106e-23 miRNAWalker2_validate;miRTarBase -0.237387253520266 3.06104626134544e-30 21115742 Forced miR-200 expression suppressed Flt1 levels in metastasis-prone lung adenocarcinoma cells derived from K-ras/p53-mutant mice and negatively regulated the Flt1 3'-UTR in reporter assays; We conclude that miR-200 suppresses lung tumorigenesis by targeting Flt1 metastasis;tumorigenesis lung cancer hsa-miR-200c-3p FLT1 2.07038651806183 6.84613386286599e-51 -0.976682151789582 4.79604479745106e-23 miRNAWalker2_validate;miRTarBase -0.255668357647663 5.50361375778068e-29 NA NA NA hsa-miR-28-5p FLYWCH1 -0.14267073663385 0.0497619246260571 0.12389624476354 0.117705319180408 miRanda -0.229517717967346 7.89386614037077e-10 NA NA NA hsa-miR-132-3p FRY -0.0365213889238607 0.668598301088667 -1.13354365828084 2.35583827383817e-18 miRNAWalker2_validate -0.144520699616158 0.00708495916317562 NA NA NA hsa-miR-28-5p FUT8 -0.14267073663385 0.0497619246260571 0.726014580237623 6.08952916861957e-11 miRanda -0.425606323456591 5.65560664857273e-16 NA NA NA hsa-miR-28-5p GALNT10 -0.14267073663385 0.0497619246260571 0.227833941975381 0.0563309072028891 miRanda -0.324201567304107 9.22307992385576e-09 NA NA NA hsa-miR-28-5p GALNT6 -0.14267073663385 0.0497619246260571 1.53253468503729 1.95615996419132e-15 miRanda -0.690592453180032 6.97388105280589e-14 NA NA NA hsa-miR-132-3p GATA3 -0.0365213889238607 0.668598301088667 1.5576496597582 2.58751948867418e-11 miRNAWalker2_validate -0.623902878863514 4.61869800965351e-11 NA NA NA hsa-miR-200a-3p GATA6 2.55218480823434 2.96135446816238e-51 -0.699851594860171 1.92456260924552e-07 miRNAWalker2_validate -0.127017900812787 7.63206405009383e-07 NA NA NA hsa-miR-28-5p GATAD1 -0.14267073663385 0.0497619246260571 -0.118787232382459 0.0381461411955589 miRanda -0.220960335790874 1.44173216936157e-16 NA NA NA hsa-miR-28-5p GATM -0.14267073663385 0.0497619246260571 -0.791153922275685 1.74092462595563e-07 miRanda -0.205174678583206 0.00473288183325133 NA NA NA hsa-miR-28-5p GCM1 -0.14267073663385 0.0497619246260571 0.360191323603245 0.134565410281452 miRanda -0.488569605079757 1.91929018001998e-05 NA NA NA hsa-miR-28-5p GIPR -0.14267073663385 0.0497619246260571 0.017258044160555 0.935509804568678 miRanda -0.685109906674805 8.75524546773542e-12 NA NA NA hsa-miR-28-5p GKAP1 -0.14267073663385 0.0497619246260571 -0.51386963839469 2.55435086487334e-08 miRanda -0.154887800263099 0.000460548342740559 NA NA NA hsa-miR-145-5p GOLM1 -2.62703571936687 7.64402900188551e-88 1.26468872915984 1.10705867867101e-25 miRNAWalker2_validate -0.219019492251804 1.50214750904505e-14 NA NA NA hsa-miR-28-5p GOSR1 -0.14267073663385 0.0497619246260571 0.030365365851841 0.621004932234963 miRanda -0.101953135923577 0.000463895107781679 NA NA NA hsa-miR-28-5p GPBP1 -0.14267073663385 0.0497619246260571 -0.0137041815437682 0.770311932798486 miRanda -0.127042004309214 8.6747456269106e-09 NA NA NA hsa-miR-28-5p GPD2 -0.14267073663385 0.0497619246260571 0.210900888733789 0.0264157292786502 miRanda -0.129869118527292 0.00409414238800342 NA NA NA hsa-let-7e-5p GPM6B 0.261471269338985 0.00191077095550257 -2.23343254430802 1.51570824751064e-39 miRNAWalker2_validate -0.294956154652224 5.04296724371224e-05 NA NA NA hsa-miR-28-5p GRAMD1A -0.14267073663385 0.0497619246260571 1.10999274616443 2.6714856402589e-53 miRanda -0.111835566009971 0.00232890461745794 NA NA NA hsa-miR-28-5p GRAMD1C -0.14267073663385 0.0497619246260571 -0.696224877463089 2.42950429103508e-08 miRanda -0.175710217376617 0.00336197515953319 NA NA NA hsa-miR-28-5p GRIA2 -0.14267073663385 0.0497619246260571 -1.22821710537384 0.00759618405734023 miRanda -0.972213489728838 8.80044759415615e-06 NA NA NA hsa-miR-132-3p GSTCD -0.0365213889238607 0.668598301088667 0.519181234576788 2.42559606422446e-07 miRNAWalker2_validate -0.173937489459421 2.06686265546553e-05 NA NA NA hsa-miR-28-5p GSTCD -0.14267073663385 0.0497619246260571 0.519181234576788 2.42559606422446e-07 miRanda -0.273053868148414 1.15459678054143e-08 NA NA NA hsa-miR-28-5p GSTT2 -0.14267073663385 0.0497619246260571 -0.191330811443633 0.355850883029681 miRanda -0.220314719845183 0.0256737201615651 NA NA NA hsa-miR-28-5p HHAT -0.14267073663385 0.0497619246260571 0.644196417869949 1.52387006114795e-08 miRanda -0.377612142840797 2.72687205190048e-12 NA NA NA hsa-miR-28-5p HK2 -0.14267073663385 0.0497619246260571 -0.0567451434395441 0.607921539997626 miRanda -0.2522359977272 1.46926915548621e-06 NA NA NA hsa-miR-28-5p HMGCL -0.14267073663385 0.0497619246260571 0.119677157715048 0.0987034936096925 miRanda -0.186094982487334 5.51594548336389e-08 NA NA NA hsa-miR-28-5p HOXB3 -0.14267073663385 0.0497619246260571 -0.0860590412997158 0.642417857654296 miRanda -0.475202338351595 5.93635891671727e-08 NA NA NA hsa-miR-141-3p HOXB5 2.99777540204168 1.26370498493024e-78 0.302409356822888 0.214170316650101 miRNAWalker2_validate -0.12785333431879 0.00689890084074076 NA NA NA hsa-miR-200b-3p HOXB5 2.11744072703209 1.66710572104191e-43 0.302409356822888 0.214170316650101 miRNAWalker2_validate -0.13052183114126 0.0115081039515208 NA NA NA hsa-miR-200c-3p HOXB5 2.07038651806183 6.84613386286599e-51 0.302409356822888 0.214170316650101 miRNAWalker2_validate -0.225962472200039 6.62396588937751e-05 NA NA NA hsa-miR-143-3p HRAS -1.23248181631499 4.74160976678504e-28 0.871315771945202 9.54693368299171e-15 miRNAWalker2_validate;miRTarBase -0.13171025180707 9.59955008801748e-05 21276449 The Evi1 microRNA 143 K Ras axis in colon cancer colon cancer hsa-miR-28-5p IER3 -0.14267073663385 0.0497619246260571 1.45642417730307 4.17853765486836e-24 miRanda -0.445532576838926 1.77892706208939e-10 NA NA NA hsa-miR-28-5p IKBKAP -0.14267073663385 0.0497619246260571 0.0789492756746029 0.15612858735694 miRanda -0.118612871400018 6.65742475118864e-06 NA NA NA hsa-miR-28-5p IKBKB -0.14267073663385 0.0497619246260571 0.273066826249678 0.00234033685126725 miRanda -0.249159305195741 4.36298401628208e-09 NA NA NA hsa-miR-28-5p IL13RA1 -0.14267073663385 0.0497619246260571 0.0785365367680315 0.282318167449858 miRanda -0.146588109721817 2.29274382682583e-05 NA NA NA hsa-miR-28-5p IMPDH1 -0.14267073663385 0.0497619246260571 0.896084693787804 4.77040402047121e-25 miRNAWalker2_validate;miRanda -0.101168477468638 0.0174266284653124 NA NA NA hsa-miR-28-5p INPP5J -0.14267073663385 0.0497619246260571 0.586361668320023 0.00503623021199427 miRanda -0.777616097841152 2.31907281894163e-15 NA NA NA hsa-miR-28-5p IQSEC2 -0.14267073663385 0.0497619246260571 0.43925999202862 1.87323493794481e-07 miRanda -0.168166530507332 2.97911256467842e-05 NA NA NA hsa-miR-28-5p ISCU -0.14267073663385 0.0497619246260571 -0.474318624658276 2.67102425402634e-20 miRanda -0.115854970892749 3.22684462403418e-06 NA NA NA hsa-miR-28-5p ITGA3 -0.14267073663385 0.0497619246260571 -0.299252352501844 0.0107499356635658 miRanda -0.36126889309902 6.72512700126372e-11 NA NA NA hsa-miR-28-5p IVD -0.14267073663385 0.0497619246260571 -0.0240972756498818 0.810416205879283 miRanda -0.461574514514484 3.38114554736114e-23 NA NA NA hsa-miR-200c-3p JAG1 2.07038651806183 6.84613386286599e-51 -0.639848689982383 1.267264807356e-11 miRNAWalker2_validate -0.198884904510323 7.69822940625124e-20 NA NA NA hsa-miR-28-5p JKAMP -0.14267073663385 0.0497619246260571 0.0735788909422048 0.22789285081931 miRanda -0.24997489116713 1.33099887959275e-18 NA NA NA hsa-miR-28-5p JPH3 -0.14267073663385 0.0497619246260571 1.09335026757806 1.5377591231963e-07 miRanda -0.259615856533199 0.00943315433708511 NA NA NA hsa-miR-132-3p KBTBD3 -0.0365213889238607 0.668598301088667 -0.676006524299838 3.2587187746144e-17 miRNAWalker2_validate -0.175161552215921 9.82281597954954e-08 NA NA NA hsa-miR-200b-3p KDR 2.11744072703209 1.66710572104191e-43 -1.26178488366313 1.81783097872036e-35 miRNAWalker2_validate;miRTarBase -0.262999260406618 3.87053169124588e-34 NA NA NA hsa-miR-28-5p KIAA0100 -0.14267073663385 0.0497619246260571 -0.0958458902820514 0.226748853303652 miRanda -0.103750602698321 0.00593347169014391 NA NA NA hsa-miR-28-5p KIAA0319L -0.14267073663385 0.0497619246260571 0.399937221728762 2.70410247773894e-07 miRanda -0.290985152725794 2.02934336200034e-15 NA NA NA hsa-miR-28-5p KIAA1328 -0.14267073663385 0.0497619246260571 -0.669584991112985 6.66171801821041e-21 miRanda -0.101475073882874 0.00354775169440602 NA NA NA hsa-miR-141-3p KLF11 2.99777540204168 1.26370498493024e-78 -1.36252050722545 2.98418037545873e-64 miRNAWalker2_validate -0.18130465417437 2.09390327369665e-28 NA NA NA hsa-miR-200a-3p KLF11 2.55218480823434 2.96135446816238e-51 -1.36252050722545 2.98418037545873e-64 miRNAWalker2_validate -0.172554627390354 1.02405720032334e-26 NA NA NA hsa-miR-200b-3p KLF11 2.11744072703209 1.66710572104191e-43 -1.36252050722545 2.98418037545873e-64 miRNAWalker2_validate -0.18136928772325 7.25506007385319e-24 NA NA NA hsa-miR-200c-3p KLF11 2.07038651806183 6.84613386286599e-51 -1.36252050722545 2.98418037545873e-64 miRNAWalker2_validate -0.189720595439794 1.24875216845081e-21 NA NA NA hsa-miR-200c-3p KLF9 2.07038651806183 6.84613386286599e-51 -1.7429680316518 2.98275437403582e-61 miRNAWalker2_validate;miRTarBase -0.370308003675866 1.40559263229779e-49 NA NA NA hsa-miR-28-5p KLHL12 -0.14267073663385 0.0497619246260571 0.529262424359425 8.20108802188939e-16 miRNAWalker2_validate -0.149641356268714 2.33277459558367e-06 NA NA NA hsa-miR-141-3p KLHL20 2.99777540204168 1.26370498493024e-78 -0.580940687803831 3.29734241071236e-18 miRNAWalker2_validate -0.131933278614168 1.09740859260953e-24 NA NA NA hsa-miR-200a-3p KLHL20 2.55218480823434 2.96135446816238e-51 -0.580940687803831 3.29734241071236e-18 miRNAWalker2_validate -0.126435474737735 1.458203736018e-23 NA NA NA hsa-miR-200b-3p KLHL20 2.11744072703209 1.66710572104191e-43 -0.580940687803831 3.29734241071236e-18 miRNAWalker2_validate -0.135017451194605 9.47763581851314e-22 NA NA NA hsa-miR-200c-3p KLHL20 2.07038651806183 6.84613386286599e-51 -0.580940687803831 3.29734241071236e-18 miRNAWalker2_validate -0.159871356307668 3.57478729778656e-25 NA NA NA hsa-miR-143-3p KRAS -1.23248181631499 4.74160976678504e-28 0.46818988509622 3.00748217900386e-10 miRNAWalker2_validate;miRTarBase -0.124135749972866 1.80319700574441e-08 22804917;27725862;19137007;26581910;23173124;26824186;21197560 In KRAS mutated tumours increased miR-200b and decreased miR-143 expression were associated with a good PFS;The expression of miR-143 is often down-regulated and it might play an important role by targeting KRAS in colorectal cancer CRC; Dual-luciferase reporter assays were performed to validate whether KRAS was regulated by miR-143; Dual-luciferase assays indicated that KRAS was a direct target of miR-143 as subsequently demonstrated by qPCR and western blot analysis showing that infection of SW480 cells with Ad-ZD55-miR-143 resulted in the down-regulation of KRAS at both mRNA and protein levels;Role of miR 143 targeting KRAS in colorectal tumorigenesis; In particular among a panel of presumed targets generated by in silico analysis that may interact with these aberrantly expressed miRNAs KRAS oncogene has been further experimentally validated as the target of miR-143; First an inverse correlation between KRAS protein and miR-143 in vivo was found; Second KRAS expression in Lovo cells was significantly abolished by treatment with miR-143 mimic whereas miR-143 inhibitor increased KRAS protein level; Third luciferase reporter assay confirmed that miR-143 directly recognize the 3'-untranslated region of KRAS transcripts; Finally inhibition of KRAS expression by miR-143 inhibits constitutive phosphorylation of ERK1/2; Taken together the present study provides the first evidences that miR-143 is significant in suppressing colorectal cancer cell growth through inhibition of KRAS translation;MicroRNA 143 replenishment re sensitizes colorectal cancer cells harboring mutant but not wild type KRAS to paclitaxel treatment; Our results showed that miR-143 but not let-7b increased sensitization of KRAS mutant tumor cells to paclitaxel; Furthermore transfection of miR-143 but not let-7b mimic negatively regulated the expression of mutant but not wild-type KRAS; Combination of miR-143 mimic and paclitaxel induced the onset of apoptosis and reverted in vitro metastatic properties migration and invasion in KRAS mutant tumor cells; MiR-143 thus can be used as a chemosensitizer for the treatment of KRAS mutant tumors and warrants further investigations in in vitro and pre-clinical in vivo models;In turn the level of miR-143 in CRC cells decreasing the amount of MACC1 metastasis-associated in colon cancer-1 and oncogenic KRAS protein may be utilized as a prognostic marker;Stable miR-143 or miR-145 overexpression increased cell sensitivity to cetuximab resulting in a significant increase of cetuximab-mediated ADCC independently of KRAS status;miR 143 decreases prostate cancer cells proliferation and migration and enhances their sensitivity to docetaxel through suppression of KRAS; The expression level of miR-143 and its target gene KRAS were measured by realtime PCR and western blotting respectively; Our results revealed an inverse correlation of expression between miR-143 and KRAS protein in prostate cancer samples Pearson's correlation scatter plots: R = -0.707 P < 0.05; These findings suggest that miR-143 plays an important role in prostate cancer proliferation migration and chemosensitivity by suppressing KRAS and subsequent inactivation of MAPK pathway which provides a potential development of a new approach for the treatment of prostate cancer ;;tumorigenesis;;metastasis;; colorectal cancer;colorectal cancer;colorectal cancer;colorectal cancer;colorectal cancer;colon cancer;prostate cancer hsa-miR-28-5p KRT1 -0.14267073663385 0.0497619246260571 -0.777638722715821 0.00784390063724915 miRanda -0.587015506653331 2.44860809440113e-05 NA NA NA hsa-miR-28-5p L2HGDH -0.14267073663385 0.0497619246260571 0.278240981720089 0.00050935264456272 miRanda -0.151525232157627 6.96875931303208e-05 NA NA NA hsa-miR-28-5p LDB3 -0.14267073663385 0.0497619246260571 -1.85025183125106 1.91109961605668e-38 miRanda -0.156357444186515 0.0284689138796435 NA NA NA hsa-miR-132-3p LFNG -0.0365213889238607 0.668598301088667 0.521997841611332 0.000590979407991039 miRNAWalker2_validate -0.182027871244963 0.00315968521458827 NA NA NA hsa-miR-28-5p LMBR1 -0.14267073663385 0.0497619246260571 0.257517541171637 0.000199666000515199 miRanda -0.108414415821819 0.00101794134974501 NA NA NA hsa-miR-132-3p LOXL1 -0.0365213889238607 0.668598301088667 1.49728597567933 5.44851508051121e-34 miRNAWalker2_validate -0.143121659688974 0.00594006063295867 NA NA NA hsa-miR-200c-3p LPAR1 2.07038651806183 6.84613386286599e-51 -1.20736956265381 1.93522163393847e-21 miRNAWalker2_validate -0.351153536982569 2.48589736261259e-33 NA NA NA hsa-miR-28-5p LRBA -0.14267073663385 0.0497619246260571 0.322142018050458 0.00274814169043239 miRanda -0.389047436907926 1.37870639587549e-14 NA NA NA hsa-miR-28-5p LRP10 -0.14267073663385 0.0497619246260571 -0.0382360620439677 0.56834890677188 miRanda -0.232358089130676 1.36883684380908e-13 NA NA NA hsa-miR-28-5p LRP2 -0.14267073663385 0.0497619246260571 -0.807564034442771 0.0466284114536984 miRanda -0.905474552810496 2.5991405316078e-06 NA NA NA hsa-miR-28-5p LRRC23 -0.14267073663385 0.0497619246260571 0.541913603183654 5.14487768181524e-09 miRanda -0.226789849549492 3.09023884401323e-07 NA NA NA hsa-miR-28-5p LRRC27 -0.14267073663385 0.0497619246260571 0.371914716525773 3.80448230629096e-06 miRanda -0.19704546268143 2.64263982844557e-07 NA NA NA hsa-miR-28-5p LRRC36 -0.14267073663385 0.0497619246260571 0.665572655767599 8.70102599652953e-05 miRanda -0.198204775980699 0.0145691548364303 NA NA NA hsa-miR-28-5p LRRC37A2 -0.14267073663385 0.0497619246260571 -0.416297658622351 0.00031793945065914 miRanda -0.124952509526358 0.0237623784992372 NA NA NA hsa-miR-28-5p LY6G6C -0.14267073663385 0.0497619246260571 -0.0705387494557708 0.809641114829419 miRanda -0.561824988628232 5.33804138289076e-05 NA NA NA hsa-miR-28-5p LYPD3 -0.14267073663385 0.0497619246260571 1.45248580283244 1.87328918691671e-19 miRanda -0.424567607281884 5.19217038033921e-08 NA NA NA hsa-miR-145-5p LYPLA2 -2.62703571936687 7.64402900188551e-88 1.03867651066728 2.15051388059863e-42 miRNAWalker2_validate -0.194085545884774 5.6183448939167e-27 NA NA NA hsa-miR-28-5p MAGI1 -0.14267073663385 0.0497619246260571 -1.26305301454899 4.04392522280675e-34 miRanda -0.116369081008409 0.0239851302853459 NA NA NA hsa-miR-28-5p MANEAL -0.14267073663385 0.0497619246260571 1.38973021457614 4.18422007286845e-24 miRanda -0.206194475853567 0.00217111584739488 NA NA NA hsa-miR-28-5p MAP1A -0.14267073663385 0.0497619246260571 -0.89250845462883 6.21955962799672e-15 miRanda -0.149629797196379 0.00694585072219689 NA NA NA hsa-miR-28-5p MARCH9 -0.14267073663385 0.0497619246260571 0.898335673282321 1.98480673766822e-29 miRanda -0.209424733313088 8.51235136745986e-08 NA NA NA hsa-miR-28-5p MARVELD2 -0.14267073663385 0.0497619246260571 1.12359621955454 1.74234529410055e-20 miRanda -0.411412447084188 1.81375532045569e-12 NA NA NA hsa-miR-28-5p MAT2A -0.14267073663385 0.0497619246260571 0.106201174005506 0.0211943042401361 miRanda -0.117893335118824 5.76635278592976e-08 NA NA NA hsa-miR-28-5p MAX -0.14267073663385 0.0497619246260571 0.189520873245771 3.41527676404311e-05 miRanda -0.126434947821145 4.87379608519139e-09 NA NA NA hsa-miR-28-5p MAZ -0.14267073663385 0.0497619246260571 1.53688020659836 2.44860030148443e-84 miRanda -0.16614489256984 6.82417495538852e-05 NA NA NA hsa-miR-143-3p MDM2 -1.23248181631499 4.74160976678504e-28 0.134052426741699 0.110706751806459 miRNAWalker2_validate -0.116551192492011 2.45785814867103e-06 NA NA NA hsa-miR-28-5p MED24 -0.14267073663385 0.0497619246260571 0.522193665864754 1.4256037186934e-07 miRanda -0.136336954991214 0.00416518893178145 NA NA NA hsa-miR-28-5p MED29 -0.14267073663385 0.0497619246260571 0.407823011340064 6.51199119798712e-14 miRanda -0.166233170354608 1.57916262167401e-10 NA NA NA hsa-miR-28-5p MEGF9 -0.14267073663385 0.0497619246260571 -0.296058092250146 0.0106105936693003 miRanda -0.275598713069223 5.27830665815295e-07 NA NA NA hsa-miR-28-5p MESDC1 -0.14267073663385 0.0497619246260571 0.579182445134239 2.26276233786632e-17 miRanda -0.100322410657288 0.00245928979734788 NA NA NA hsa-miR-28-5p MID2 -0.14267073663385 0.0497619246260571 -0.799925928617615 7.05192180338706e-20 miRanda -0.132368175980547 0.00192648108990026 NA NA NA hsa-miR-145-5p MMP1 -2.62703571936687 7.64402900188551e-88 6.06794406171332 1.97080965138208e-60 miRNAWalker2_validate -0.776560200165228 1.4991690670364e-17 NA NA NA hsa-miR-28-5p MMP11 -0.14267073663385 0.0497619246260571 6.04945497433362 1.03023931867355e-137 miRanda -0.429299903434847 0.00172361360383875 NA NA NA hsa-miR-145-5p MMP12 -2.62703571936687 7.64402900188551e-88 1.47938135981664 1.47098262596235e-05 miRNAWalker2_validate -0.192036777823138 0.0160531504579215 NA NA NA hsa-miR-143-3p MMP13 -1.23248181631499 4.74160976678504e-28 6.71640412670262 7.74215774273361e-81 miRNAWalker2_validate -0.508349675625 1.03503351857542e-05 21427707;24762226;23732700 Western blot analyses revealed that MMP-13 was mostly protein downregulated by miR-143;Moreover the overexpression of miR-143 decreased MMP-13 protein level; Finally the neutralization of miR-143 by anti-miR-143 antibody reversed the effect of propofol on cell proliferation apoptosis and invasion and upregulated MMP-13 expression in MG63 cells; Taken together propofol may have antitumor potential in osteosarcoma which is partly due to the downregulation of MMP-13 expression by miR-143;MicroRNA 143 inhibits cell migration and invasion by targeting matrix metalloproteinase 13 in prostate cancer; In addition to the best of our knowledge miR-143 was reported for the first time to directly target matrix metalloproteinase 13 MMP-13 in prostate cancer; The results of the present study demonstrated that miR-143 suppresses cell migration and invasion by targeting MMP-13 in prostate cancer cell lines ;;cell migration sarcoma;sarcoma;prostate cancer hsa-miR-145-5p MMP14 -2.62703571936687 7.64402900188551e-88 1.17709675366481 4.88698053995378e-22 miRNAWalker2_validate -0.100472607295886 0.00054272881478419 NA NA NA hsa-miR-28-5p MORF4L2 -0.14267073663385 0.0497619246260571 0.754044361942787 2.09810836739711e-29 miRanda -0.148347261398627 6.48658571412929e-06 NA NA NA hsa-miR-28-5p MOV10L1 -0.14267073663385 0.0497619246260571 -0.297466473208126 0.0920646073093874 miRanda -0.200559727756046 0.017178490274872 NA NA NA hsa-miR-28-5p MPI -0.14267073663385 0.0497619246260571 0.028970874839473 0.6353259007928 miRanda -0.168930693478824 4.35470728606857e-09 NA NA NA hsa-let-7e-5p MPL 0.261471269338985 0.00191077095550257 -1.55729613911731 1.15011006095171e-60 miRNAWalker2_validate -0.108150095010754 0.009759901565627 NA NA NA hsa-miR-28-5p MR1 -0.14267073663385 0.0497619246260571 0.327436070496887 0.000151124551464722 miRanda -0.11465704514548 0.00545481477812533 NA NA NA hsa-miR-28-5p MRPL17 -0.14267073663385 0.0497619246260571 1.00679596033877 1.95241823571098e-45 miRanda -0.107583871355843 0.00267303465044058 NA NA NA hsa-miR-28-5p MRPS35 -0.14267073663385 0.0497619246260571 0.546529477360202 2.2953747137935e-18 miRanda -0.11368310884075 0.000176002275848875 NA NA NA hsa-miR-28-5p MS4A2 -0.14267073663385 0.0497619246260571 -0.791944427086409 0.00109280176197654 miRanda -1.00321375313711 9.28749255223754e-19 NA NA NA hsa-miR-200c-3p MSN 2.07038651806183 6.84613386286599e-51 -0.578039968356322 4.29885633617379e-10 miRNAWalker2_validate;miRTarBase -0.193434786532672 1.25331591841618e-19 NA NA NA hsa-miR-145-5p MUC1 -2.62703571936687 7.64402900188551e-88 2.6031293995538 1.72038877386261e-27 miRNAWalker2_validate;miRTarBase -0.381041291185968 2.23197939904781e-11 24157791 MiR 145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1; MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3'UTRs; Importantly the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145 respectively; Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1 functioning as a tumor suppressor ovarian cancer hsa-miR-28-5p MUC2 -0.14267073663385 0.0497619246260571 2.26355229838227 1.52647808582801e-07 miRanda -1.17344429096599 1.12063804571833e-08 NA NA NA hsa-miR-28-5p MUM1 -0.14267073663385 0.0497619246260571 -0.126556156553718 0.0782751092469258 miRanda -0.129631276755681 0.000144830852269932 NA NA NA hsa-miR-28-5p MUM1L1 -0.14267073663385 0.0497619246260571 -0.158739344936463 0.536343388622944 miRanda -0.704525822706198 6.29784602575918e-09 NA NA NA hsa-miR-28-5p MYB -0.14267073663385 0.0497619246260571 1.3385004671291 4.8746375657764e-10 miRanda -0.793948725008808 6.86876365185527e-15 NA NA NA hsa-miR-28-5p MYCBPAP -0.14267073663385 0.0497619246260571 0.270395883018356 0.168169145671457 miRanda -0.521583955632841 1.89815971070376e-08 NA NA NA hsa-miR-28-5p MYEF2 -0.14267073663385 0.0497619246260571 0.553427331133231 2.34778821065553e-05 miRanda -0.154420149585165 0.0136797924479402 NA NA NA hsa-miR-28-5p MYLIP -0.14267073663385 0.0497619246260571 -0.57505884257391 2.93951647319766e-11 miRanda -0.271745980842622 4.32764111087329e-11 NA NA NA hsa-miR-28-5p MYO5C -0.14267073663385 0.0497619246260571 -0.148719986034905 0.196143460079208 miRanda -0.387154085850764 8.02386753686477e-13 NA NA NA hsa-miR-143-3p MYO6 -1.23248181631499 4.74160976678504e-28 0.64462966298129 1.058698364996e-10 miRNAWalker2_validate;miRTarBase -0.185502933006214 3.59686893777106e-10 20353999 A computational search indicated the 3'-untranslated region UTR of the mRNA for myosin VI MYO6 as a potential target for both miR-143 and miR-145 the expression of which was reduced in the tumor tissues; In luciferase reporter analysis we find a significant negative regulatory effect on the MYO6 3'UTR by both miR-143 and miR-145; Mutation of the potential binding sites for miR-143 and miR-145 in the MYO6 3'UTR resulted in a loss of responsiveness to the corresponding miRNA; Our data indicate that miR-143 and miR-145 are involved in the regulation of MYO6 expression and possibly in the development of prostate cancer prostate cancer hsa-miR-145-5p MYO6 -2.62703571936687 7.64402900188551e-88 0.64462966298129 1.058698364996e-10 miRNAWalker2_validate;miRTarBase -0.116693579773699 5.6139791172851e-07 20353999 A computational search indicated the 3'-untranslated region UTR of the mRNA for myosin VI MYO6 as a potential target for both miR-143 and miR-145 the expression of which was reduced in the tumor tissues; In luciferase reporter analysis we find a significant negative regulatory effect on the MYO6 3'UTR by both miR-143 and miR-145; Mutation of the potential binding sites for miR-143 and miR-145 in the MYO6 3'UTR resulted in a loss of responsiveness to the corresponding miRNA; Our data indicate that miR-143 and miR-145 are involved in the regulation of MYO6 expression and possibly in the development of prostate cancer prostate cancer hsa-miR-28-5p MYOZ3 -0.14267073663385 0.0497619246260571 -0.711322284940144 0.000149018891882461 miRanda -0.341141244970893 0.000137356928333869 NA NA NA hsa-miR-28-5p MYT1 -0.14267073663385 0.0497619246260571 3.12383178963035 2.84482084584268e-20 miRanda -0.858419706373622 1.75550265549345e-07 NA NA NA hsa-miR-28-5p NAGPA -0.14267073663385 0.0497619246260571 0.476491606622916 3.65876806231442e-10 miRanda -0.100081318214584 0.00618236613995102 NA NA NA hsa-miR-28-5p NAV1 -0.14267073663385 0.0497619246260571 -0.159057359476455 0.12674105203339 miRanda -0.148433487492913 0.00274439035375534 NA NA NA hsa-miR-200c-3p NCAM1 2.07038651806183 6.84613386286599e-51 -2.68069543446954 3.3168998510845e-39 miRTarBase -0.537372709763967 1.49098248094642e-28 NA NA NA hsa-miR-28-5p NDE1 -0.14267073663385 0.0497619246260571 0.875339226885404 7.92857237232874e-36 miRanda -0.103552782556862 0.00294271629492361 NA NA NA hsa-miR-28-5p NDUFS4 -0.14267073663385 0.0497619246260571 -0.301526713163377 1.63649105931826e-06 miRanda -0.15098636267489 4.48455928241219e-07 NA NA NA hsa-miR-28-5p NECAP1 -0.14267073663385 0.0497619246260571 0.194681737356563 0.00227095720937965 miRanda -0.157152843023967 1.95426624970528e-07 NA NA NA hsa-miR-28-5p NEIL2 -0.14267073663385 0.0497619246260571 -0.0448079050208445 0.544773057514391 miRanda -0.119176975433056 0.000691336086998745 NA NA NA hsa-miR-28-5p NEK11 -0.14267073663385 0.0497619246260571 0.128069407523834 0.188727293195575 miRanda -0.207811713330311 6.84039361090454e-06 NA NA NA hsa-miR-28-5p NEK9 -0.14267073663385 0.0497619246260571 -0.60343465464345 2.91093269226533e-23 miRanda -0.211923929411196 4.69742347617414e-13 NA NA NA hsa-miR-28-5p NELL1 -0.14267073663385 0.0497619246260571 0.559671843191762 0.100112035151243 miRanda -0.443668109807395 0.00622745471416146 NA NA NA hsa-miR-28-5p NF1 -0.14267073663385 0.0497619246260571 -0.394546709839459 1.88157518863882e-05 miRanda -0.10961770733041 0.0129522477508265 NA NA NA hsa-miR-145-5p NIPSNAP1 -2.62703571936687 7.64402900188551e-88 1.15064562678506 4.6913047115871e-55 miRNAWalker2_validate -0.229313090452633 2.0106869959855e-39 NA NA NA hsa-miR-28-5p NKAIN1 -0.14267073663385 0.0497619246260571 3.58782119473036 3.15479732457313e-27 miRanda -1.02962002614055 2.05084781347217e-10 NA NA NA hsa-miR-28-5p NLK -0.14267073663385 0.0497619246260571 0.568183653225175 4.68554951142873e-14 miRanda -0.182841180159811 4.4803820168759e-07 NA NA NA hsa-miR-28-5p NPNT -0.14267073663385 0.0497619246260571 1.30537723017584 8.93205976672538e-11 miRanda -0.721622294532342 4.48461531437353e-14 NA NA NA hsa-miR-28-5p NRSN2 -0.14267073663385 0.0497619246260571 1.0077861189057 1.12746017285371e-32 miRanda -0.248146104353577 2.4591706014397e-09 NA NA NA hsa-miR-200c-3p NTF3 2.07038651806183 6.84613386286599e-51 -2.35193065681614 1.75397805257351e-24 miRNAWalker2_validate -0.405535845133949 1.08735324650309e-13 NA NA NA hsa-miR-28-5p NTM -0.14267073663385 0.0497619246260571 0.221487289190883 0.123560204043128 miRanda -0.157071376888052 0.0219271809832237 NA NA NA hsa-miR-200c-3p NTRK2 2.07038651806183 6.84613386286599e-51 -4.03565225718056 9.2986992242195e-64 miRNAWalker2_validate;miRTarBase -0.540459062838814 7.12630091174492e-20 23209748;23074172 miR 200c sensitizes breast cancer cells to doxorubicin treatment by decreasing TrkB and Bmi1 expression;miR-200c also targets TrkB a mediator of resistance to anoikis ;drug resistance breast cancer;ovarian cancer hsa-miR-28-5p NUAK1 -0.14267073663385 0.0497619246260571 -0.914610809156931 1.8807905701441e-17 miRanda -0.131373331691557 0.0120066009154216 NA NA NA hsa-miR-28-5p NUAK2 -0.14267073663385 0.0497619246260571 2.20281585866602 3.89561828942553e-79 miRanda -0.37596435617299 7.22431489231184e-10 NA NA NA hsa-miR-28-5p NUCB2 -0.14267073663385 0.0497619246260571 0.670385380475985 8.68806334102954e-09 miRanda -0.280586626471 4.66620702358173e-07 NA NA NA hsa-miR-145-5p NUDT1 -2.62703571936687 7.64402900188551e-88 1.1327988039844 9.21559149300805e-27 miRTarBase -0.256925946439927 1.83830847661055e-25 21289483 MiR 145 inhibits cell proliferation of human lung adenocarcinoma by targeting EGFR and NUDT1; The mRNA expressions of EGFR and NUDT1 were significantly downregulated after miR-145 transfection in human lung adenocarcinoma cells; Our results demonstrated miR-145 in the negative regulation of EGFR and NUDT1 expressions at both mRNA and protein levels; Upregulation of miR-145 appeared to be an important gene regulation mechanism for the proliferation of lung adenocarcinoma cells and it correlated strongly with the downregulation of EGFR and NUDT1; Our findings provided new insight into the complex regulating pathway comprising of miR-145 EGFR NUDT1 and other unknown factors which function in cell proliferation but not in apoptosis lung cancer hsa-miR-28-5p NUDT13 -0.14267073663385 0.0497619246260571 -0.458684101138813 1.49139282091863e-06 miRanda -0.124661710754265 0.00631011761644721 NA NA NA hsa-let-7e-5p NUDT8 0.261471269338985 0.00191077095550257 -0.13517503992748 0.428648427443961 miRNAWalker2_validate -0.363889825067688 1.58385949691265e-07 NA NA NA hsa-miR-132-3p OAS2 -0.0365213889238607 0.668598301088667 1.17346164591443 5.93578964327051e-14 miRNAWalker2_validate -0.267095614113891 3.08705948967938e-05 NA NA NA hsa-miR-28-5p OLFM1 -0.14267073663385 0.0497619246260571 -0.806833993567473 2.33080631095537e-05 miRanda -0.434330191870437 1.74743829260296e-06 NA NA NA hsa-miR-28-5p OLFML3 -0.14267073663385 0.0497619246260571 -0.484779181334281 4.26190141017196e-05 miRanda -0.146634750872719 0.00964142489546047 NA NA NA hsa-miR-28-5p OSBPL10 -0.14267073663385 0.0497619246260571 -0.0654040479624038 0.460318540732145 miRanda -0.114725848755068 0.00646868684724235 NA NA NA hsa-miR-28-5p OSBPL7 -0.14267073663385 0.0497619246260571 0.0588084177089319 0.550542377722673 miRanda -0.160386070710978 0.000610888019257143 NA NA NA hsa-miR-132-3p OSBPL8 -0.0365213889238607 0.668598301088667 -0.597116166688471 4.67806844867655e-09 miRNAWalker2_validate -0.122348190300177 0.00328898023138446 NA NA NA hsa-miR-28-5p OTUB1 -0.14267073663385 0.0497619246260571 0.743708114973462 2.76727756237135e-34 miRNAWalker2_validate;miRTarBase -0.114501198557697 0.000145943210829922 NA NA NA hsa-miR-28-5p OTUB2 -0.14267073663385 0.0497619246260571 1.04819143562664 1.51953841962343e-30 miRanda -0.149357921381267 0.000915308983616309 NA NA NA hsa-miR-28-5p PAFAH2 -0.14267073663385 0.0497619246260571 0.274540087069485 2.25730801999012e-05 miRanda -0.181474588639603 3.33470892114403e-09 NA NA NA hsa-miR-145-5p PAK4 -2.62703571936687 7.64402900188551e-88 1.47186288644382 7.37718156197043e-58 miRNAWalker2_validate;miRTarBase -0.326408303596423 4.52801469396177e-52 23499891 We further demonstrated that miR-145 directly targeted catenin δ-1 contributing to the aberrant translocation of β-catenin through impaired nuclear shuttling with p21-activated kinase 4 PAK4 colon cancer hsa-miR-28-5p PAN2 -0.14267073663385 0.0497619246260571 -0.417044459562392 2.76743472392804e-09 miRanda -0.17925739666463 8.53714401819021e-08 NA NA NA hsa-miR-28-5p PCDH10 -0.14267073663385 0.0497619246260571 -0.589847783454892 0.0702331583145207 miRanda -0.538818734654809 0.000511157836185964 NA NA NA hsa-miR-28-5p PCSK6 -0.14267073663385 0.0497619246260571 0.33169561953974 0.0771775975214687 miRanda -0.325068253320658 0.000271228066827847 NA NA NA hsa-miR-28-5p PCYOX1 -0.14267073663385 0.0497619246260571 -1.09884998986409 7.48295373511972e-39 miRanda -0.135347671586706 0.00129452200167461 NA NA NA hsa-miR-28-5p PCYOX1L -0.14267073663385 0.0497619246260571 0.554225987753898 5.61819495077454e-16 miRanda -0.190735646180457 6.71716832065865e-09 NA NA NA hsa-miR-28-5p PDE4A -0.14267073663385 0.0497619246260571 -0.10844696857969 0.381039125696737 miRanda -0.227281107486484 0.000110708900233232 NA NA NA hsa-miR-28-5p PDGFRB -0.14267073663385 0.0497619246260571 -0.363053423609744 0.000930448280520273 miRanda -0.106080049561343 0.0428915822633876 NA NA NA hsa-miR-28-5p PDXDC1 -0.14267073663385 0.0497619246260571 0.867679818835133 1.64124821154065e-33 miRanda -0.20421343833345 7.92479068630763e-09 NA NA NA hsa-miR-28-5p PEX12 -0.14267073663385 0.0497619246260571 -0.244701247391237 0.00249870618819211 miRanda -0.269940810394297 1.38504482563852e-12 NA NA NA hsa-miR-28-5p PFKM -0.14267073663385 0.0497619246260571 -0.167575311099228 0.0685593901261937 miRanda -0.116393774331497 0.00788145315345331 NA NA NA hsa-miR-28-5p PHKB -0.14267073663385 0.0497619246260571 -0.474811813850328 3.42461136633396e-10 miRanda -0.111196268170306 0.0022176267680483 NA NA NA hsa-miR-28-5p PHKG2 -0.14267073663385 0.0497619246260571 1.17584598160819 7.15388386958662e-52 miRanda -0.201461578928291 2.7542518948257e-07 NA NA NA hsa-miR-28-5p PIGG -0.14267073663385 0.0497619246260571 0.107993711309021 0.0641318638666071 miRanda -0.156799939519724 1.20811913049496e-08 NA NA NA hsa-miR-28-5p PIGO -0.14267073663385 0.0497619246260571 0.632201925119523 4.99557211163621e-21 miRanda -0.189309260037223 5.74895962733358e-09 NA NA NA hsa-miR-28-5p PIK3R2 -0.14267073663385 0.0497619246260571 1.28293433832686 8.5972816204378e-73 miRanda -0.170225138245223 4.06181677270291e-06 NA NA NA hsa-miR-28-5p PIK3R3 -0.14267073663385 0.0497619246260571 0.559172960265555 3.98014986716526e-09 miRanda -0.112036735701901 0.0141687305542462 NA NA NA hsa-miR-28-5p PIP4K2C -0.14267073663385 0.0497619246260571 0.785545156337934 1.33112420136684e-29 miRanda -0.201400552827145 3.19497519827677e-09 NA NA NA hsa-miR-28-5p PLCD4 -0.14267073663385 0.0497619246260571 0.135354827465335 0.311825654751022 miRanda -0.503044438055499 9.77066808847889e-16 NA NA NA hsa-miR-28-5p PLEKHH1 -0.14267073663385 0.0497619246260571 -0.261980470817113 0.0364210745908391 miRanda -0.142488673730933 0.0169848340831966 NA NA NA hsa-miR-28-5p PMPCA -0.14267073663385 0.0497619246260571 0.52698682555574 5.78301134193271e-17 miRanda -0.119185297110825 9.09650178789744e-05 NA NA NA hsa-miR-132-3p POLK -0.0365213889238607 0.668598301088667 -0.643740598724049 1.80887720530213e-15 miRNAWalker2_validate -0.106925678594733 0.00132163197833319 NA NA NA hsa-miR-141-3p PPARA 2.99777540204168 1.26370498493024e-78 -1.66915873925688 2.97363179285551e-46 miRNAWalker2_validate;miRTarBase -0.247904615736715 2.4815889948105e-26 NA NA NA hsa-miR-28-5p PPIP5K1 -0.14267073663385 0.0497619246260571 -0.194364800594986 0.0237079880541731 miRanda -0.314004798274111 6.46576353569024e-15 NA NA NA hsa-miR-200c-3p PPM1F 2.07038651806183 6.84613386286599e-51 -1.01782278266201 2.82903184176664e-41 miRNAWalker2_validate -0.203540907484171 6.22609794635238e-30 22144583 MicroRNA 200c represses migration and invasion of breast cancer cells by targeting actin regulatory proteins FHOD1 and PPM1F; We identified FHOD1 and PPM1F direct regulators of the actin cytoskeleton as novel targets of miR-200c; Remarkably expression levels of FHOD1 and PPM1F were inversely correlated with the level of miR-200c in breast cancer cell lines breast cancer patient samples and 58 cancer cell lines of various origins breast cancer hsa-miR-28-5p PPOX -0.14267073663385 0.0497619246260571 0.830291250171679 6.58170304361639e-23 miRanda -0.169004189934174 3.92854986223783e-05 NA NA NA hsa-miR-28-5p PPP1R14D -0.14267073663385 0.0497619246260571 1.08406784337159 4.61757779337715e-07 miRanda -0.688171627902918 1.5008747175405e-11 NA NA NA hsa-miR-28-5p PREPL -0.14267073663385 0.0497619246260571 -0.322293281183161 8.79993670730062e-06 miRanda -0.11687031971652 0.000735961886475128 NA NA NA hsa-miR-28-5p PROM2 -0.14267073663385 0.0497619246260571 1.56071419150459 1.77985184998625e-25 miRanda -0.346665083892436 2.17099288160034e-06 NA NA NA hsa-miR-28-5p PRR5-ARHGAP8 -0.14267073663385 0.0497619246260571 1.48757303661783 5.00986879889586e-23 miRanda -0.204597767552065 0.00551521866120443 NA NA NA hsa-miR-141-3p PTEN 2.99777540204168 1.26370498493024e-78 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase -0.12717832397844 6.47904977288109e-18 27644195;24742567 Involvement of microRNA 141 3p in 5 fluorouracil and oxaliplatin chemo resistance in esophageal cancer cells via regulation of PTEN; Western blot exhibited altered protein levels of PTEN Akt and PI3k with miR-141-3p inhibitor; An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples; Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo;PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis drug resistance;tumorigenesis esophageal cancer;endometrial cancer hsa-miR-28-5p PTER -0.14267073663385 0.0497619246260571 -0.191994453096763 0.0246991311855573 miRanda -0.133337486918225 0.00104206341060228 NA NA NA hsa-miR-28-5p PTGER3 -0.14267073663385 0.0497619246260571 -2.08536199219359 1.19288336263977e-20 miRanda -0.536055938428695 8.25873008564214e-07 NA NA NA hsa-miR-28-5p PTP4A2 -0.14267073663385 0.0497619246260571 0.38574449513147 1.13328117872512e-06 miRanda -0.227965984611241 1.35730245928027e-09 NA NA NA hsa-miR-200c-3p PTPN13 2.07038651806183 6.84613386286599e-51 -0.809960140594897 7.91282502499025e-08 miRNAWalker2_validate;miRTarBase -0.18590883585444 1.33149075961008e-07 NA NA NA hsa-miR-141-3p PTPRD 2.99777540204168 1.26370498493024e-78 0.150114854210014 0.427806011089459 miRNAWalker2_validate -0.252890035581267 3.43971076968601e-12 NA NA NA hsa-miR-200a-3p PTPRD 2.55218480823434 2.96135446816238e-51 0.150114854210014 0.427806011089459 miRNAWalker2_validate -0.264438339347084 1.0759495244519e-13 NA NA NA hsa-miR-200b-3p PTPRD 2.11744072703209 1.66710572104191e-43 0.150114854210014 0.427806011089459 miRNAWalker2_validate -0.30522980195377 1.14494479230952e-14 NA NA NA hsa-miR-200c-3p PTPRD 2.07038651806183 6.84613386286599e-51 0.150114854210014 0.427806011089459 miRNAWalker2_validate -0.341096545199508 4.09077043221735e-15 NA NA NA hsa-miR-28-5p PUS7L -0.14267073663385 0.0497619246260571 0.145113950566918 0.0897960737939671 miRanda -0.114959118446961 0.00473506724081946 NA NA NA hsa-miR-28-5p PYCRL -0.14267073663385 0.0497619246260571 1.82468595902489 2.66248549409042e-58 miRanda -0.22504000152473 0.000103907331598124 NA NA NA hsa-miR-28-5p QSOX1 -0.14267073663385 0.0497619246260571 0.60255748274154 3.62670004110163e-08 miRanda -0.152628390389117 0.00364426402193613 NA NA NA hsa-miR-28-5p RAB11FIP1 -0.14267073663385 0.0497619246260571 0.69116808896398 6.48076941128492e-06 miRanda -0.338905684912959 3.5216293612899e-06 NA NA NA hsa-miR-28-5p RAB14 -0.14267073663385 0.0497619246260571 -0.0753658943653273 0.14399081719528 miRanda -0.104162677236291 1.96703121287158e-05 NA NA NA hsa-miR-28-5p RAB24 -0.14267073663385 0.0497619246260571 0.303475540325776 0.000592601062267745 miRanda -0.161893953740376 0.000118862111928195 NA NA NA hsa-miR-28-5p RAB30 -0.14267073663385 0.0497619246260571 -0.478979836326187 0.00389471393081148 miRanda -0.459147966639985 5.06933876170286e-09 NA NA NA hsa-miR-28-5p RAB31 -0.14267073663385 0.0497619246260571 1.21770647114932 2.0308057149131e-20 miRanda -0.203782492807051 0.00148128168670373 NA NA NA hsa-miR-28-5p RALGAPB -0.14267073663385 0.0497619246260571 0.114339916324857 0.110018355395065 miRanda -0.136336527884099 5.88496299374839e-05 NA NA NA hsa-miR-28-5p RAP1GDS1 -0.14267073663385 0.0497619246260571 0.478268842738917 4.89838888082789e-09 miRanda -0.179884587821433 4.21195745918722e-06 NA NA NA hsa-miR-28-5p RAPGEFL1 -0.14267073663385 0.0497619246260571 0.740048213907199 1.05350561263932e-05 miRanda -0.284270875346076 0.000399239787482548 NA NA NA hsa-miR-28-5p RASGRP4 -0.14267073663385 0.0497619246260571 -0.655281786974586 6.53684341783125e-09 miRanda -0.1170558792494 0.0311401193861443 NA NA NA hsa-miR-141-3p RASSF2 2.99777540204168 1.26370498493024e-78 -0.0757663600101672 0.471968773241686 miRNAWalker2_validate -0.135873264046898 1.85145668024501e-11 NA NA NA hsa-miR-200a-3p RASSF2 2.55218480823434 2.96135446816238e-51 -0.0757663600101672 0.471968773241686 miRNAWalker2_validate -0.122092807456322 8.31045841692893e-10 NA NA NA hsa-miR-200b-3p RASSF2 2.11744072703209 1.66710572104191e-43 -0.0757663600101672 0.471968773241686 miRNAWalker2_validate -0.132962009202936 1.87793486378845e-09 NA NA NA hsa-miR-200c-3p RASSF2 2.07038651806183 6.84613386286599e-51 -0.0757663600101672 0.471968773241686 miRNAWalker2_validate -0.15915180997094 5.64117330111008e-11 NA NA NA hsa-miR-28-5p RBM47 -0.14267073663385 0.0497619246260571 0.732126183413737 2.07916919204205e-13 miRanda -0.336368008731163 1.55575789347527e-12 NA NA NA hsa-miR-28-5p RBM5 -0.14267073663385 0.0497619246260571 -0.402544753547652 4.49201522260434e-13 miRanda -0.117173839370532 1.14468263555622e-05 NA NA NA hsa-let-7e-5p RDX 0.261471269338985 0.00191077095550257 -0.777241607854884 9.59924790525334e-16 miRNAWalker2_validate -0.122623435373547 0.00232117136821428 NA NA NA hsa-miR-28-5p REEP1 -0.14267073663385 0.0497619246260571 0.546339756833729 0.0100889448892189 miRanda -0.801186934216671 8.69273047648115e-16 NA NA NA hsa-miR-28-5p RHOT1 -0.14267073663385 0.0497619246260571 -0.270568764875986 8.26985055768295e-05 miRanda -0.107030886738767 0.00109224356536059 NA NA NA hsa-miR-28-5p RIMS4 -0.14267073663385 0.0497619246260571 1.93936813754878 1.60005847788467e-09 miRanda -1.19551474512527 4.05189387001245e-15 NA NA NA hsa-miR-200c-3p RIN2 2.07038651806183 6.84613386286599e-51 -0.13319809102269 0.125216128478784 miRNAWalker2_validate -0.107502630238496 9.09693498682217e-08 NA NA NA hsa-miR-28-5p RMST -0.14267073663385 0.0497619246260571 -2.44382192551357 5.91433025323596e-08 miRanda -0.449675579460211 0.00994127001403051 NA NA NA hsa-miR-200b-3p RND3 2.11744072703209 1.66710572104191e-43 -1.70606216262212 4.40038671490463e-36 miRNAWalker2_validate;miRTarBase -0.198068196895994 3.25777991523227e-11 26935796 MicroRNA 200b inhibits epithelial mesenchymal transition and migration of cervical cancer cells by directly targeting RhoE cervical and endocervical cancer hsa-miR-28-5p RNF10 -0.14267073663385 0.0497619246260571 0.183922227767296 7.82104616512277e-06 miRanda -0.14080061678934 3.19560723383641e-13 NA NA NA hsa-miR-28-5p RNF187 -0.14267073663385 0.0497619246260571 1.01633369097037 2.51780917868843e-52 miRanda -0.142009177223535 2.67776542852784e-05 NA NA NA hsa-miR-28-5p RPLP0P2 -0.14267073663385 0.0497619246260571 2.37092895765011 4.02584451390848e-74 miRanda -0.188975627128783 0.00552319763125875 NA NA NA hsa-miR-28-5p RPS6KA5 -0.14267073663385 0.0497619246260571 -0.957245810152851 9.30300768011881e-15 miRanda -0.174299109370714 0.00356769791900598 NA NA NA hsa-miR-28-5p RRM2B -0.14267073663385 0.0497619246260571 0.104156612356596 0.252644515445598 miRanda -0.182015578311515 2.50011313753227e-05 NA NA NA hsa-miR-145-5p RTKN -2.62703571936687 7.64402900188551e-88 1.29492093513268 4.15162188834918e-51 miRNAWalker2_validate -0.2656003480696 5.30374903814574e-39 19360360 miR 145 inhibits breast cancer cell growth through RTKN; Subsequently RTKN is identified as a potential miR-145 target by bioinformatics; Using reporter constructs we show that the RTKN 3' untranslated region 3'UTR carries the directly binding site of miR-145; Additionally overexpression of miR-145 in MCF-7 reduces RTKN protein expression as well as mRNA level; Taken together we propose that loss of miR-145 may provide a selective growth advantage for MCF-7 by targeting RTKN breast cancer hsa-miR-132-3p RTN4 -0.0365213889238607 0.668598301088667 -0.601034406966253 3.97552525003937e-25 miRNAWalker2_validate -0.116553766615971 1.26920618717024e-06 NA NA NA hsa-miR-28-5p RTN4RL1 -0.14267073663385 0.0497619246260571 0.0235441317845835 0.90079236818366 miRanda -0.876981766929322 1.19675645433379e-23 NA NA NA hsa-miR-28-5p RUNDC1 -0.14267073663385 0.0497619246260571 0.295842513834232 0.000580189934087065 miRanda -0.341668097976478 2.11469014255674e-17 NA NA NA hsa-miR-28-5p RWDD2A -0.14267073663385 0.0497619246260571 0.125683302205475 0.0854271541365872 miRanda -0.126326393967917 0.000271630782118608 NA NA NA hsa-miR-28-5p SCAMP4 -0.14267073663385 0.0497619246260571 0.614297407813703 1.11749160548696e-19 miRanda -0.22839424792154 2.22663220756444e-12 NA NA NA hsa-miR-28-5p SCAMP5 -0.14267073663385 0.0497619246260571 0.907126239257327 6.22969102491787e-15 miRanda -0.176739481873598 0.00169322267288296 NA NA NA hsa-miR-28-5p SCUBE1 -0.14267073663385 0.0497619246260571 -1.08277169869843 7.17388900019697e-05 miRanda -0.353971159496076 0.00665611802321946 NA NA NA hsa-miR-28-5p SEC16A -0.14267073663385 0.0497619246260571 0.705401856519326 1.92241123956851e-20 miRanda -0.339102192526052 8.28289847991014e-21 NA NA NA hsa-miR-28-5p SEC23IP -0.14267073663385 0.0497619246260571 0.204057998350036 0.00401511033994364 miRanda -0.135069810091733 6.12254771224588e-05 NA NA NA hsa-miR-28-5p SEMA4B -0.14267073663385 0.0497619246260571 0.85463500471788 2.10181293576613e-16 miRanda -0.209395080388177 3.172581117495e-05 NA NA NA hsa-miR-28-5p SERHL -0.14267073663385 0.0497619246260571 -0.645855068242783 0.00196100425848289 miRanda -0.229659952245983 0.00409157837596331 NA NA NA hsa-miR-28-5p SERHL2 -0.14267073663385 0.0497619246260571 -0.343857304888721 0.211470376872396 miRanda -0.539276926118838 3.69430260584961e-05 NA NA NA hsa-miR-28-5p SERPINA1 -0.14267073663385 0.0497619246260571 0.396626436447673 0.0640799863509273 miRanda -0.203400299005591 0.0465295085588463 NA NA NA hsa-miR-28-5p SERPINA5 -0.14267073663385 0.0497619246260571 -0.519209583936095 0.103183128111987 miRanda -0.774799437153621 2.86498994052742e-07 NA NA NA hsa-miR-28-5p SESN1 -0.14267073663385 0.0497619246260571 -0.952506930201764 9.2781623761265e-26 miRanda -0.176225999962169 7.4172729512754e-05 NA NA NA hsa-miR-28-5p SETD3 -0.14267073663385 0.0497619246260571 -0.269540757095891 6.11415488403297e-09 miRanda -0.120126356803789 5.06213015407305e-08 NA NA NA hsa-miR-28-5p SETD7 -0.14267073663385 0.0497619246260571 -1.05533462896746 5.56168604809659e-30 miRNAWalker2_validate -0.133362643708109 0.00359921691003603 NA NA NA hsa-miR-28-5p SFI1 -0.14267073663385 0.0497619246260571 0.732803203984513 3.09771867450456e-15 miRanda -0.118946816609226 0.00822629167514562 NA NA NA hsa-miR-28-5p SFXN1 -0.14267073663385 0.0497619246260571 1.00730972646257 1.50510712985854e-31 miRanda -0.247575672122889 4.90943877345944e-09 NA NA NA hsa-miR-132-3p SFXN2 -0.0365213889238607 0.668598301088667 1.08971879328793 7.30694562473144e-18 miRNAWalker2_validate -0.185076162608237 0.000396745017311297 NA NA NA hsa-miR-28-5p SFXN2 -0.14267073663385 0.0497619246260571 1.08971879328793 7.30694562473144e-18 miRanda -0.338083961196289 3.19538617411812e-08 NA NA NA hsa-miR-28-5p SGSH -0.14267073663385 0.0497619246260571 0.131555704868576 0.0379109975142145 miRanda -0.100760708072422 0.000816389719327486 NA NA NA hsa-miR-28-5p SGSM2 -0.14267073663385 0.0497619246260571 -0.226680479630394 0.0136805050128053 miRanda -0.114807613503313 0.00877829877218787 NA NA NA hsa-miR-132-3p SH3BGRL -0.0365213889238607 0.668598301088667 0.268820390705558 0.0442547470608479 miRNAWalker2_validate -0.348825621436921 7.45784338503323e-11 NA NA NA hsa-miR-28-5p SH3BGRL -0.14267073663385 0.0497619246260571 0.268820390705558 0.0442547470608479 miRanda -0.203847085630723 0.00135374949702198 NA NA NA hsa-miR-28-5p SHISA2 -0.14267073663385 0.0497619246260571 -0.0881092399519519 0.754073621031887 miRanda -0.396778641338464 0.00303480221724045 NA NA NA hsa-miR-28-5p SHISA6 -0.14267073663385 0.0497619246260571 -1.68226082206879 2.45685318014832e-13 miRanda -0.234893998087409 0.0346973294930808 NA NA NA hsa-miR-28-5p SIPA1L3 -0.14267073663385 0.0497619246260571 1.14456261546297 2.77744871058553e-40 miRanda -0.169840461616851 7.87079640561921e-05 NA NA NA hsa-miR-132-3p SIRT1 -0.0365213889238607 0.668598301088667 -0.599221126327341 9.41183428987515e-17 miRNAWalker2_validate;miRTarBase -0.102468817666313 0.000556327150746778 NA NA NA hsa-miR-28-5p SIRT3 -0.14267073663385 0.0497619246260571 0.0107489864050141 0.879499666544636 miRanda -0.26212648335716 2.62798701565363e-15 NA NA NA hsa-miR-28-5p SLC1A7 -0.14267073663385 0.0497619246260571 -1.89898960979584 3.58937473841316e-24 miRanda -0.203026000903691 0.0271945182255877 NA NA NA hsa-miR-28-5p SLC24A1 -0.14267073663385 0.0497619246260571 -0.404947269243294 2.78114264738042e-07 miRanda -0.204201346532967 5.23054938284372e-08 NA NA NA hsa-miR-28-5p SLC25A38 -0.14267073663385 0.0497619246260571 0.217477580021136 0.000198540649760527 miRanda -0.206918858185028 4.85128860783084e-14 NA NA NA hsa-miR-28-5p SLC25A45 -0.14267073663385 0.0497619246260571 0.176528287332551 0.0319167814740833 miRanda -0.210753344836521 6.12562039555599e-08 NA NA NA hsa-miR-28-5p SLC44A5 -0.14267073663385 0.0497619246260571 -0.219753996293499 0.440105379643692 miRanda -0.605333732419013 7.37553109686195e-06 NA NA NA hsa-miR-28-5p SLC6A4 -0.14267073663385 0.0497619246260571 0.832715997717498 0.0306047620635539 miRanda -0.671833824606927 0.000247165301550305 NA NA NA hsa-miR-28-5p SMARCC2 -0.14267073663385 0.0497619246260571 -0.227365550061606 4.75275814043985e-07 miRanda -0.140280402202475 4.7343458860196e-11 NA NA NA hsa-miR-28-5p SMUG1 -0.14267073663385 0.0497619246260571 0.606414386600416 3.40733965995423e-19 miRanda -0.224300530222957 5.5767010594015e-12 NA NA NA hsa-miR-28-5p SNAP23 -0.14267073663385 0.0497619246260571 -0.00190318843343817 0.971884090942836 miRanda -0.133008859520424 1.76795790321163e-07 NA NA NA hsa-miR-28-5p SNRPN -0.14267073663385 0.0497619246260571 -0.145756373517547 0.194678771019692 miRanda -0.264927747076875 6.445156224726e-07 NA NA NA hsa-miR-28-5p SNX13 -0.14267073663385 0.0497619246260571 -0.47494994622097 8.24215014257509e-11 miRanda -0.114077424788396 0.00117219357673941 NA NA NA hsa-miR-28-5p SNX19 -0.14267073663385 0.0497619246260571 -0.332901048450884 1.43819698027206e-07 miRanda -0.111921626247476 0.00021533004209152 NA NA NA hsa-miR-28-5p SORCS1 -0.14267073663385 0.0497619246260571 -1.17464209650385 0.00185228663810649 miRanda -0.461554834766473 0.010437753492585 NA NA NA hsa-miR-145-5p SOX2 -2.62703571936687 7.64402900188551e-88 1.62824878642595 1.83270623627945e-06 miRNAWalker2_validate;miRTarBase -0.406343012519686 3.02930538603098e-07 20382729;21211035;22098779;25951106;22835608 Finally we provide evidence that EWS-FLI-1 and miRNA-145 function in a mutually repressive feedback loop and identify their common target gene SOX2 in addition to miRNA145 itself as key players in ESFT cell differentiation and tumorigenicity;We also show that miR-145 and SOX2 form a double negative feedback loop in GBM cells potentially creating a bistable system in GBM cells;In this study our miRNA/mRNA-microarray and RT-PCR analysis showed that the expression of miR145 a tumor-suppressive miRNA is inversely correlated with the levels of Oct4 and Sox2 in GBM-CD133+ cells and malignant glioma specimens; We demonstrated that miR145 negatively regulates GBM tumorigenesis by targeting Oct4 and Sox2 in GBM-CD133+;Overexpression of miR 145 5p inhibits proliferation of prostate cancer cells and reduces SOX2 expression; We proposed that miR-145-5p being an important regulator of SOX2 carries a crucial role in PCa tumorigenesis;miR-145 regulates SOX2 and OCT4 translation and p53 regulates miR-145 expression differentiation;;malignant trasformation;tumorigenesis;tumorigenesis; sarcoma;glioblastoma;glioblastoma;prostate cancer;lung squamous cell cancer hsa-miR-28-5p SPAG1 -0.14267073663385 0.0497619246260571 1.21536502852341 1.21867718052225e-27 miRanda -0.283385630238123 2.1349786014205e-07 NA NA NA hsa-miR-28-5p SPARC -0.14267073663385 0.0497619246260571 0.260111874990256 0.034213916848521 miRanda -0.128260261137617 0.0285436818829644 NA NA NA hsa-miR-28-5p SPATA2 -0.14267073663385 0.0497619246260571 0.422408617204912 3.11792520748896e-12 miRanda -0.131824028628949 5.76197976366993e-06 NA NA NA hsa-miR-28-5p SPATA20 -0.14267073663385 0.0497619246260571 0.44118485989573 8.656953078129e-05 miRanda -0.500777774110776 1.23992642174534e-21 NA NA NA hsa-miR-28-5p SPATA5L1 -0.14267073663385 0.0497619246260571 0.178145637609615 0.00049083620708605 miRanda -0.102077664728975 2.56348708697251e-05 NA NA NA hsa-miR-28-5p SPEF2 -0.14267073663385 0.0497619246260571 0.0180087182537036 0.892221023811872 miRanda -0.441848875618062 1.50726561843833e-12 NA NA NA hsa-let-7e-5p SPN 0.261471269338985 0.00191077095550257 0.0101792377906609 0.943004960884371 miRNAWalker2_validate -0.190776437753625 0.00103187988120867 NA NA NA hsa-miR-28-5p SPNS1 -0.14267073663385 0.0497619246260571 0.863726955106674 4.25847180628551e-32 miRanda -0.133019080595056 0.000236964129139015 NA NA NA hsa-miR-28-5p SQSTM1 -0.14267073663385 0.0497619246260571 0.337430896843218 6.84019405897406e-06 miRanda -0.184705196991679 2.22156890650776e-07 NA NA NA hsa-miR-28-5p SRCIN1 -0.14267073663385 0.0497619246260571 1.01769401933761 6.04876320869876e-10 miRanda -0.442078106154846 1.85478243537203e-08 NA NA NA hsa-miR-28-5p SSBP2 -0.14267073663385 0.0497619246260571 -0.688186904830189 6.78722403001534e-13 miRanda -0.298326289348499 7.2427398305492e-11 NA NA NA hsa-miR-145-5p STAT1 -2.62703571936687 7.64402900188551e-88 0.84704946611897 1.52503088632031e-12 miRNAWalker2_validate;miRTarBase -0.190431855359047 8.49731192294897e-12 20098684 MicroRNA 145 targets YES and STAT1 in colon cancer cells; Both YES and STAT1 were verified as direct miR-145 targets colon cancer hsa-miR-28-5p STC1 -0.14267073663385 0.0497619246260571 0.0174437759200483 0.927534883709258 miRanda -0.420438503201195 3.76779887199724e-06 NA NA NA hsa-miR-28-5p STK16 -0.14267073663385 0.0497619246260571 0.402504151002991 9.90775556523305e-12 miRanda -0.188797357633773 1.9631444476179e-11 NA NA NA hsa-miR-28-5p STK36 -0.14267073663385 0.0497619246260571 0.377236149506294 6.85193611730421e-06 miRanda -0.22144955704607 2.71205283096417e-08 NA NA NA hsa-miR-28-5p STOM -0.14267073663385 0.0497619246260571 -0.649999293476048 7.55025346293203e-11 miRanda -0.145691676720967 0.00243906835735245 NA NA NA hsa-miR-28-5p STRADB -0.14267073663385 0.0497619246260571 -0.643341720695693 4.11123485334864e-15 miRanda -0.154073446657361 9.90677268422725e-05 NA NA NA hsa-miR-28-5p SYBU -0.14267073663385 0.0497619246260571 -0.572312328428279 0.00218363989087214 miRanda -0.730310895089721 7.30811267796815e-17 NA NA NA hsa-miR-28-5p SYCE2 -0.14267073663385 0.0497619246260571 1.83143884411464 1.42387265552736e-34 miRanda -0.236968196033737 0.00140462299853398 NA NA NA hsa-miR-28-5p SYNC -0.14267073663385 0.0497619246260571 -0.153981352248731 0.325479128978546 miRanda -0.265851291789184 0.000355254741684812 NA NA NA hsa-miR-28-5p SYT13 -0.14267073663385 0.0497619246260571 2.91774638341289 1.4380254498439e-12 miRanda -0.705964334486968 0.000384111843790838 NA NA NA hsa-miR-28-5p SYTL4 -0.14267073663385 0.0497619246260571 -0.26631400934631 0.0393829139575531 miRanda -0.456650470025982 5.39086292604251e-14 NA NA NA hsa-miR-28-5p TANC2 -0.14267073663385 0.0497619246260571 0.645784897636979 5.11106873490809e-07 miRanda -0.241961164649315 8.34629992228275e-05 NA NA NA hsa-miR-28-5p TAOK2 -0.14267073663385 0.0497619246260571 0.433430948462074 3.95755269074194e-12 miRanda -0.25995291139941 1.14651902610124e-18 NA NA NA hsa-miR-28-5p TARS2 -0.14267073663385 0.0497619246260571 1.02415963397561 8.39575134988038e-47 miRanda -0.122295473108918 0.000663078548652261 NA NA NA hsa-miR-28-5p TAT -0.14267073663385 0.0497619246260571 -4.1700211703831 1.44104525158907e-18 miRanda -0.477313247620828 0.038945109081538 NA NA NA hsa-miR-132-3p TBC1D9 -0.0365213889238607 0.668598301088667 0.311454954290858 0.115143760209625 miRNAWalker2_validate -0.337736020344779 2.32246246169984e-05 NA NA NA hsa-miR-28-5p TBX2 -0.14267073663385 0.0497619246260571 0.129922787207087 0.243301824733681 miRanda -0.140703521954603 0.00794171052002852 NA NA NA hsa-miR-28-5p TC2N -0.14267073663385 0.0497619246260571 0.0475147882231264 0.727197958425978 miRanda -0.184267973480312 0.00446523882844074 NA NA NA hsa-miR-141-3p TCF7L1 2.99777540204168 1.26370498493024e-78 -1.43757533529955 1.98162937384072e-20 miRNAWalker2_validate -0.199972471420516 6.28430074006789e-11 NA NA NA hsa-miR-200a-3p TCF7L1 2.55218480823434 2.96135446816238e-51 -1.43757533529955 1.98162937384072e-20 miRNAWalker2_validate -0.150558299078396 6.03400969336786e-07 NA NA NA hsa-miR-200b-3p TCF7L1 2.11744072703209 1.66710572104191e-43 -1.43757533529955 1.98162937384072e-20 miRNAWalker2_validate -0.168143697293025 5.41553060367198e-07 NA NA NA hsa-miR-200c-3p TCF7L1 2.07038651806183 6.84613386286599e-51 -1.43757533529955 1.98162937384072e-20 miRNAWalker2_validate -0.229533628135833 4.20710186780113e-10 NA NA NA hsa-miR-28-5p TCTA -0.14267073663385 0.0497619246260571 0.20213938843336 0.00613628359574618 miRanda -0.198402744330933 1.27950291789586e-08 NA NA NA hsa-miR-28-5p TEX14 -0.14267073663385 0.0497619246260571 0.931242979008404 9.10679550289147e-05 miRanda -0.36038453972202 0.00152927774957985 NA NA NA hsa-miR-28-5p TEX9 -0.14267073663385 0.0497619246260571 0.0509689822456678 0.700082142899211 miRanda -0.1670711645078 0.00798015597379457 NA NA NA hsa-miR-141-3p TGFB2 2.99777540204168 1.26370498493024e-78 -0.568239705690126 0.00206510120463672 miRNAWalker2_validate;miRTarBase -0.201139839320628 1.72029232417547e-08 NA NA NA hsa-miR-28-5p THRB -0.14267073663385 0.0497619246260571 -1.90956725674414 3.33207199356048e-40 miRanda -0.27319149495517 0.000145463303643686 NA NA NA hsa-miR-28-5p TIMM17B -0.14267073663385 0.0497619246260571 1.26177930091032 3.653096676374e-45 miRanda -0.130289288298862 0.00383630002885173 NA NA NA hsa-miR-200c-3p TIMP2 2.07038651806183 6.84613386286599e-51 -0.790977606892998 7.91966411856086e-15 miRTarBase -0.348884866594025 7.49589633579639e-54 NA NA NA hsa-miR-28-5p TM9SF1 -0.14267073663385 0.0497619246260571 0.511606899292477 9.1955728473893e-18 miRanda -0.215783534058051 3.66881145727977e-14 NA NA NA hsa-miR-28-5p TMCO1 -0.14267073663385 0.0497619246260571 0.851360857509368 3.5933761176123e-38 miRanda -0.113140925721111 0.000567460927779503 NA NA NA hsa-miR-28-5p TMED3 -0.14267073663385 0.0497619246260571 1.5516937076501 5.27191563318331e-68 miRanda -0.161128244421002 0.000478744777849298 NA NA NA hsa-miR-28-5p TMEM143 -0.14267073663385 0.0497619246260571 -0.466637204908091 3.9291975727201e-11 miRanda -0.213576348163273 2.24806769006338e-10 NA NA NA hsa-miR-28-5p TMEM144 -0.14267073663385 0.0497619246260571 -0.222974365100777 0.0453860472712897 miRanda -0.391648808451717 7.14375923521578e-14 NA NA NA hsa-miR-28-5p TMEM167A -0.14267073663385 0.0497619246260571 0.0809111072909596 0.146434441090959 miRanda -0.111664828448186 2.29709460466846e-05 NA NA NA hsa-miR-28-5p TMEM215 -0.14267073663385 0.0497619246260571 2.65768345418265 2.3521799065528e-20 miRanda -0.555439357324754 7.2589393236591e-05 NA NA NA hsa-miR-28-5p TMEM229B -0.14267073663385 0.0497619246260571 0.727265089473085 3.07635214377309e-08 miRanda -0.429646253572464 5.46749176294498e-12 NA NA NA hsa-miR-28-5p TMEM9 -0.14267073663385 0.0497619246260571 1.17225854097205 9.42118512805465e-50 miRanda -0.122673788326945 0.00217777126125373 NA NA NA hsa-miR-28-5p TMPRSS4 -0.14267073663385 0.0497619246260571 3.35879061803581 9.9015112131537e-20 miRanda -0.503325088395346 0.00522533815614918 NA NA NA hsa-miR-28-5p TMUB1 -0.14267073663385 0.0497619246260571 1.12105172023654 7.11663811953612e-29 miRanda -0.152750765888196 0.00205613729174163 NA NA NA hsa-miR-28-5p TNK1 -0.14267073663385 0.0497619246260571 0.739466980555803 1.02517411574197e-12 miRanda -0.320837899015955 9.84933977086471e-11 NA NA NA hsa-miR-28-5p TOM1L1 -0.14267073663385 0.0497619246260571 0.704932194727521 7.70954564884351e-09 miRanda -0.356251331334049 8.93759550117042e-10 NA NA NA hsa-miR-28-5p TOX3 -0.14267073663385 0.0497619246260571 1.00432770054387 0.00104894619135282 miRanda -0.896029443840828 6.53496646773437e-10 NA NA NA hsa-miR-145-5p TPM3 -2.62703571936687 7.64402900188551e-88 1.20094351262857 1.52351376875333e-75 miRNAWalker2_validate -0.199366546874454 8.34701725003177e-36 NA NA NA hsa-miR-132-3p TRIM23 -0.0365213889238607 0.668598301088667 -0.683201005926275 2.66644008303317e-19 miRNAWalker2_validate -0.113726078056972 0.000295292870797658 NA NA NA hsa-miR-28-5p TRIM23 -0.14267073663385 0.0497619246260571 -0.683201005926275 2.66644008303317e-19 miRanda -0.24115237133317 4.6089734472748e-11 NA NA NA hsa-miR-28-5p TRPS1 -0.14267073663385 0.0497619246260571 0.975779250773293 1.00018911688075e-11 miRanda -0.141435859063669 0.0410592316645809 NA NA NA hsa-miR-28-5p TRUB2 -0.14267073663385 0.0497619246260571 0.674503131886807 1.91964910840085e-28 miRanda -0.167148321435792 2.03074836591556e-08 NA NA NA hsa-miR-28-5p TSG101 -0.14267073663385 0.0497619246260571 0.296712396871171 9.16329380962956e-09 miRanda -0.151996263076067 5.58777477793531e-10 NA NA NA hsa-miR-28-5p TSNAX -0.14267073663385 0.0497619246260571 0.422819928987759 7.41936878269938e-12 miRanda -0.124763553794833 2.54924890571281e-05 NA NA NA hsa-miR-28-5p TTC25 -0.14267073663385 0.0497619246260571 0.0268474985965776 0.854483881724695 miRanda -0.246590592818799 0.000393070449956777 NA NA NA hsa-miR-28-5p TTC39A -0.14267073663385 0.0497619246260571 1.75127730029576 1.95458566556964e-23 miRanda -0.561215658602864 4.32187154676238e-11 NA NA NA hsa-miR-28-5p TTC39C -0.14267073663385 0.0497619246260571 0.148888218233073 0.119005357502186 miRanda -0.238856584253465 1.2467565465088e-07 NA NA NA hsa-miR-28-5p TTC8 -0.14267073663385 0.0497619246260571 0.178398576559903 0.035079271986482 miRanda -0.380737846963701 3.34153417132127e-22 NA NA NA hsa-miR-28-5p TUBGCP5 -0.14267073663385 0.0497619246260571 -0.192566353644787 0.00132433306189554 miRanda -0.107054440107932 0.000173050564531711 NA NA NA hsa-miR-28-5p TUFM -0.14267073663385 0.0497619246260571 0.727504297935931 6.51865293930577e-27 miRNAWalker2_validate -0.163308818860107 8.17892052902472e-07 NA NA NA hsa-miR-28-5p TXNDC15 -0.14267073663385 0.0497619246260571 -0.12678933462949 0.0100887464640449 miRanda -0.106882127419917 4.56302344280308e-06 NA NA NA hsa-miR-28-5p UBE2Z -0.14267073663385 0.0497619246260571 0.531416701885103 1.79902342102429e-16 miRanda -0.126906932637718 4.71304832612847e-05 NA NA NA hsa-miR-28-5p UBE3B -0.14267073663385 0.0497619246260571 -0.04341053843455 0.44320588192684 miRanda -0.20876431830344 2.90771228545953e-15 NA NA NA hsa-miR-28-5p UBTD2 -0.14267073663385 0.0497619246260571 0.128941566560699 0.0252656180967694 miRanda -0.113783835802965 3.10751419447168e-05 NA NA NA hsa-miR-28-5p UBTF -0.14267073663385 0.0497619246260571 0.480221215287978 7.43410138383196e-26 miRanda -0.110317015872406 7.05826550992748e-07 NA NA NA hsa-miR-28-5p ULK3 -0.14267073663385 0.0497619246260571 0.735573512973171 1.0601693976613e-25 miRanda -0.126486106001449 0.000233852849670499 NA NA NA hsa-miR-28-5p URGCP -0.14267073663385 0.0497619246260571 0.0526536004239073 0.285595768643589 miRanda -0.148657531101123 1.3587095350023e-10 NA NA NA hsa-miR-28-5p URM1 -0.14267073663385 0.0497619246260571 0.389004206411093 5.19312203313837e-12 miRanda -0.102999514913133 0.000142527195319968 NA NA NA hsa-miR-28-5p VAV3 -0.14267073663385 0.0497619246260571 1.34411719441904 1.087241773222e-13 miRanda -0.611662799107083 1.47436473807405e-12 NA NA NA hsa-miR-200a-3p VCAM1 2.55218480823434 2.96135446816238e-51 -0.422080444805657 0.00361929851720551 miRNAWalker2_validate -0.192217611764734 2.18432689837879e-12 NA NA NA hsa-miR-28-5p VEZF1 -0.14267073663385 0.0497619246260571 -0.218442006456705 0.0044599756979924 miRanda -0.163030520501413 7.78947500099617e-06 NA NA NA hsa-miR-28-5p VEZT -0.14267073663385 0.0497619246260571 0.181406259391838 0.0056683713856385 miRanda -0.148894796889565 1.6576465295747e-06 NA NA NA hsa-miR-28-5p VWA3A -0.14267073663385 0.0497619246260571 0.383442103163063 0.125108467711998 miRanda -0.365623168031948 0.00213064702838744 NA NA NA hsa-miR-200a-3p WASF3 2.55218480823434 2.96135446816238e-51 -2.52846175739233 2.89361416346383e-58 miRNAWalker2_validate;miRTarBase -0.405908608720436 7.8748305448137e-40 NA NA NA hsa-miR-200b-3p WASF3 2.11744072703209 1.66710572104191e-43 -2.52846175739233 2.89361416346383e-58 miRNAWalker2_validate;miRTarBase -0.426347852136764 2.62441563344211e-35 NA NA NA hsa-miR-28-5p WBP1 -0.14267073663385 0.0497619246260571 0.580480433955926 7.12747644898469e-17 miRanda -0.227493317147737 9.00615701119687e-12 NA NA NA hsa-miR-28-5p WDR83 -0.14267073663385 0.0497619246260571 0.79199972757636 1.67453700958747e-15 miRanda -0.229298714039308 1.76029336904606e-06 NA NA NA hsa-miR-28-5p WIPF2 -0.14267073663385 0.0497619246260571 -0.00735809585132774 0.937520602697966 miRanda -0.134513318957187 0.00257432351610525 NA NA NA hsa-miR-200a-3p YAP1 2.55218480823434 2.96135446816238e-51 -1.31738265371239 9.58372053397518e-43 miRNAWalker2_validate -0.179180498954883 2.18192480130206e-21 23340296 MicroRNA 200a promotes anoikis resistance and metastasis by targeting YAP1 in human breast cancer; We identified Yes-associated protein 1 YAP1 as a novel target of miR-200a; Our data showed that targeting of YAP1 by miR-200a resulted in decreased expression of proapoptotic proteins which leads to anoikis resistance; Remarkably we found that YAP1 expression was inversely correlated with miR-200a expression in breast cancer clinical specimens and miR-200a expression was associated with distant metastasis in patients with breast cancer metastasis;drug resistance breast cancer hsa-miR-28-5p YPEL3 -0.14267073663385 0.0497619246260571 0.00907921639306331 0.92452046690186 miRanda -0.197612504863076 1.35986130569366e-05 NA NA NA hsa-miR-141-3p ZEB1 2.99777540204168 1.26370498493024e-78 -1.20177694006781 1.58231961737395e-22 miRNAWalker2_validate;miRTarBase -0.372196262043322 7.31339268489263e-60 24424572;24307282;26783187 Accordingly the enforced expression of miR-200c and mir-141 resulted in a significant upregulation in E-cadherin expression contrary to the significant downregulation in ZEB1 expression in 3 cell lines UTSCC-24A UTSCC-24B and UTSCC-6A cells;It is estimated that miR-141 played its role via ZEB1 and ZEB2; In all miR-141 played a osteosarcoma-suppressing role via ZEB1 and ZEB2;Nintedanib was able to reverse TGF-β1-induced EMT and resistance to gefitinib caused by miR-200b and miR-141 upregulation and ZEB1 downregulation ;;drug resistance head and neck cancer;sarcoma;lung squamous cell cancer hsa-miR-200a-3p ZEB1 2.55218480823434 2.96135446816238e-51 -1.20177694006781 1.58231961737395e-22 miRNAWalker2_validate;miRTarBase -0.319622679415122 1.50005867526347e-44 27402080;23626803;24037528;27402864;23743934;24815002;24424572;20473948;19854497;26887977;24390222;26242262;19544444 Conversely ectopic expression of miR-200 inhibited this sprouting by indirectly reducing the protein levels of PDGFRβ through the direct suppression of ZEB1;Concomitant with miR-200 decrease there was an increase in ZEB1 mRNA expression;MiR 200 can repress breast cancer metastasis through ZEB1 independent but moesin dependent pathways;This circuit comprises the microRNA 200 miR-200 family ZEB1 and GRHL2;This finding was accompanied by a sharp downregulation in the expression of the miR-200 family of microRNAs well-known targets of ZEB1;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;ZEB1 is also known to repress miR-200c-141 transcription in a negative feedback loop but otherwise little is known about the transcriptional regulation of the miR-200 family and miR-205;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;GRHL2 miR 200 ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification;Inhibition of ZEB1 by miR 200 characterizes Helicobacter pylori positive gastric diffuse large B cell lymphoma with a less aggressive behavior; Through genome-wide expression profiles of both miRNAs and mRNAs in nine H pylori-positive and nine H pylori-negative gastric diffuse large B-cell lymphomas we identified inhibition of ZEB1 zinc-finger E-box-binding homeobox 1 by miR-200 in H pylori-positive gastric diffuse large B-cell lymphomas; In 30 H pylori-positive and 30 H pylori-negative gastric diffuse large B-cell lymphomas we confirmed that H pylori-positive gastric diffuse large B-cell lymphomas had higher levels of miR-200 by qRT-PCR and lower levels of ZEB1 and higher levels of BCL6 using immunohistochemistry;MiR 200 suppresses metastases of colorectal cancer through ZEB1; Here we showed that the levels of miR-200 were significantly decreased and the levels of ZEB1 were significantly increased in the CRC specimens from patients compared to the paired non-tumor tissue; Moreover the levels of miR-200 and ZEB1 are inversely correlated; Bioinformatics analyses showed that miR-200 targeted the 3'-UTR of ZEB1 mRNA to inhibit its translation which was confirmed by luciferase reporter assay;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2 ;;metastasis;;;;tumorigenesis;;;;;metastasis; breast cancer;breast cancer;breast cancer;sarcoma;endometrial cancer;sarcoma;head and neck cancer;bladder cancer;ovarian cancer;ovarian cancer;B cell lymphoma;colorectal cancer;prostate cancer hsa-miR-200b-3p ZEB1 2.11744072703209 1.66710572104191e-43 -1.20177694006781 1.58231961737395e-22 miRNAWalker2_validate;miRTarBase -0.340144365122761 1.59273196940771e-40 27402080;26011542;23626803;24037528;24352645;27402864;23743934;24815002;24424572;20473948;19854497;26887977;24390222;27307751;26242262;19544444;26783187 Conversely ectopic expression of miR-200 inhibited this sprouting by indirectly reducing the protein levels of PDGFRβ through the direct suppression of ZEB1;Here we show that 53BP1 negatively regulated EMT by modulating ZEB1 through targeting microRNA miR-200b and miR-429; In 18 clinical tissue samples expression of 53BP1 was positively correlated with miR-200b and mir-429 and negatively correlated with ZEB1;Concomitant with miR-200 decrease there was an increase in ZEB1 mRNA expression; Concomitant with the increase in miR-200b and miR-200c ZEB1 expression was decreased and cells appeared more epithelial in morphology and were sensitized to TAM and fulvestrant inhibition;MiR 200 can repress breast cancer metastasis through ZEB1 independent but moesin dependent pathways;Functional experiments demonstrated that miR-200b suppressed ZEB1 augmented E-cadherin inhibited cell migration and suppressed tumor growth in a mouse model;This circuit comprises the microRNA 200 miR-200 family ZEB1 and GRHL2;This finding was accompanied by a sharp downregulation in the expression of the miR-200 family of microRNAs well-known targets of ZEB1;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;ZEB1 is also known to repress miR-200c-141 transcription in a negative feedback loop but otherwise little is known about the transcriptional regulation of the miR-200 family and miR-205;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;GRHL2 miR 200 ZEB1 maintains the epithelial status of ovarian cancer through transcriptional regulation and histone modification;Inhibition of ZEB1 by miR 200 characterizes Helicobacter pylori positive gastric diffuse large B cell lymphoma with a less aggressive behavior; Through genome-wide expression profiles of both miRNAs and mRNAs in nine H pylori-positive and nine H pylori-negative gastric diffuse large B-cell lymphomas we identified inhibition of ZEB1 zinc-finger E-box-binding homeobox 1 by miR-200 in H pylori-positive gastric diffuse large B-cell lymphomas; In 30 H pylori-positive and 30 H pylori-negative gastric diffuse large B-cell lymphomas we confirmed that H pylori-positive gastric diffuse large B-cell lymphomas had higher levels of miR-200 by qRT-PCR and lower levels of ZEB1 and higher levels of BCL6 using immunohistochemistry;MicroRNA 200b acts as a tumor suppressor in osteosarcoma via targeting ZEB1; Moreover ZEB1 was identified as a target gene of miR-200b and its expression levels were negatively mediated by miR-200b in osteosarcoma cells; Based on these findings our study suggests that miR-200b inhibits the proliferation migration and invasion of osteosarcoma cells probably via the inhibition of ZEB1 expression;MiR 200 suppresses metastases of colorectal cancer through ZEB1; Here we showed that the levels of miR-200 were significantly decreased and the levels of ZEB1 were significantly increased in the CRC specimens from patients compared to the paired non-tumor tissue; Moreover the levels of miR-200 and ZEB1 are inversely correlated; Bioinformatics analyses showed that miR-200 targeted the 3'-UTR of ZEB1 mRNA to inhibit its translation which was confirmed by luciferase reporter assay;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2;Nintedanib was able to reverse TGF-β1-induced EMT and resistance to gefitinib caused by miR-200b and miR-141 upregulation and ZEB1 downregulation ;;;metastasis;cell migration;;;;tumorigenesis;;;;;;metastasis;;drug resistance breast cancer;breast cancer;breast cancer;breast cancer;gastric cancer;sarcoma;endometrial cancer;sarcoma;head and neck cancer;bladder cancer;ovarian cancer;ovarian cancer;B cell lymphoma;sarcoma;colorectal cancer;prostate cancer;lung squamous cell cancer hsa-miR-200c-3p ZEB1 2.07038651806183 6.84613386286599e-51 -1.20177694006781 1.58231961737395e-22 miRNAWalker2_validate;miRTarBase -0.419256244047077 6.2413741334997e-52 24710933;24615544;21682933;27717206;23626803;27666124;23754305;24424572;24186205;22407310 miR-200c has been shown to regulate the epithelial-mesenchymal transition EMT by inhibiting ZEB1 and ZEB2 expression in breast cancer cells; This study further examined the role of miR-200c in the invasion and metastasis of breast cancer that goes beyond the regulation on ZEB1 and ZEB2 expression;MiR 200c suppresses TGF β signaling and counteracts trastuzumab resistance and metastasis by targeting ZNF217 and ZEB1 in breast cancer; MiR-200c which was the most significantly downregulated miRNA in trastuzumab-resistant cells restored trastuzumab sensitivity and suppressed invasion of breast cancer cells by concurrently targeting ZNF217 a transcriptional activator of TGF-β and ZEB1 a known mediator of TGF-β signaling; Given the reported backward inhibition of miR-200c by ZEB1 ZNF217 also exerts a feedback suppression of miR-200c via TGF-β/ZEB1 signaling; Restoration of miR-200c silencing of ZEB1 or ZNF217 or blockade of TGF-β signaling increased trastuzumab sensitivity and suppressed invasiveness of breast cancer cells;Of this family miR-200c has garnered particular attention as a consequence of its ability to target ZEB1 and ZEB2 mediators of epithelial- mesenchymal transition;Epigenetic Silencing of miR 200c in Breast Cancer Is Associated with Aggressiveness and Is Modulated by ZEB1;Concomitant with the increase in miR-200b and miR-200c ZEB1 expression was decreased and cells appeared more epithelial in morphology and were sensitized to TAM and fulvestrant inhibition;miR 200c regulates crizotinib resistant ALK positive lung cancer cells by reversing epithelial mesenchymal transition via targeting ZEB1;Overexpression of miR-200c in SN12-PM6 and 786-0 cells was concurrent with downregulation of ZEB1 and upregulation of E-cadherin mRNA and protein; Thus our study demonstrated that miR-200c decreases the metastatic ability of renal carcinoma cells by upregulating E-cadherin through ZEB1 and that modulating the expression of miR-200c could influence Akt protein levels;Accordingly the enforced expression of miR-200c and mir-141 resulted in a significant upregulation in E-cadherin expression contrary to the significant downregulation in ZEB1 expression in 3 cell lines UTSCC-24A UTSCC-24B and UTSCC-6A cells;Furthermore miR-200c was found to be important in modulating ZEB1 upregulation by ERG;miR 200c inhibits invasion and migration in human colon cancer cells SW480/620 by targeting ZEB1; Among those dysregulated microRNAs miR-200c was speculated to inhibit metastasis by targeting ZEB1; Overexpression of miR-200c was concurrent with downregulation of ZEB1 mRNA and protein; Taken together our study demonstrated that miR-200c inhibits metastatic ability by targeting ZEB1 in colon cancer cells SW480/620 and suggested that modulation of miR-200c could serve as therapeutic tool for inhibiting metastasis in colorectal cancer metastasis;metastasis;drug resistance;;;;;;;;metastasis breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;lung cancer;kidney renal cell cancer;head and neck cancer;prostate cancer;colon cancer hsa-miR-141-3p ZEB2 2.99777540204168 1.26370498493024e-78 -1.60369039498659 1.13296131497065e-43 miRNAWalker2_validate;miRTarBase -0.414576149422103 1.12624955121735e-83 25975736;18925646;24307282 These results suggest that miR‑141 may be involved in the inhibition of gastric cancer cell migration and that ZEB2 is a target gene of miR-141;TargetScan algorithm revealed that ZFHX1B mRNA is a hypothetical target of both miR-141 and -200c; We established by quantitative RT-PCR that in CCCs in which miR-141 and miR-200c were down-regulated ZFHX1B a transcriptional repressor for CDH1/E-cadherin tended to be up-regulated; On the basis of these findings we suggest that down-regulation of miR-141 and miR-200c in CCCs might be involved in suppression of CDH1/E-cadherin transcription via up-regulation of ZFHX1B;It is estimated that miR-141 played its role via ZEB1 and ZEB2; In all miR-141 played a osteosarcoma-suppressing role via ZEB1 and ZEB2 cell migration;; gastric cancer;kidney renal cell cancer;sarcoma hsa-miR-200a-3p ZEB2 2.55218480823434 2.96135446816238e-51 -1.60369039498659 1.13296131497065e-43 miRNAWalker2_validate;miRTarBase -0.34961751703046 1.4672767099241e-58 24518294;19787069;24815002;24424572;19854497;21529905;19544444;25797260 This switch involved activation of the transforming growth factor-β TGFβ signaling network that led to a shift in the balance between miR-200 microRNAs and the transcription factor zinc finger E-box-binding homeobox 2 ZEB2 resulting in suppressed transcription of the gene encoding E-cadherin;These findings are surprising since the miR-200 family was previously shown to promote epithelial characteristics by inhibiting the transcriptional repressor Zeb2 and thereby enhancing E-cadherin expression; Expression of miR-200 which promotes a mesenchymal to epithelial cell transition MET by inhibiting Zeb2 expression unexpectedly enhances macroscopic metastases in mouse breast cancer cell lines;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;MicroRNA 200a inhibits CD133/1+ ovarian cancer stem cells migration and invasion by targeting E cadherin repressor ZEB2; The 3'-UTR of ZEB2 mRNA a transcriptional repressor of E-cadherin was found to be regulated directly by miR-200a; Our results suggest that loss of expression of miR-200a may play a critical role in the repression of E-cadherin by ZEB2 thereby enhancing migration and invasion in CD133/1+ cells;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2;MicroRNA 200a suppresses metastatic potential of side population cells in human hepatocellular carcinoma by decreasing ZEB2; Overexpression of miR-200a in SP cells decreased metastasis-related markers and expression of ZEB2; The associations between miR-200a SP cells and ZEB2 were validated in HCC; These findings reveal that miR-200a suppresses metastasis of SP cells by downregulating ZEB2 ;metastasis;;tumorigenesis;;;;metastasis breast cancer;breast cancer;sarcoma;head and neck cancer;ovarian cancer;ovarian cancer;prostate cancer;liver cancer hsa-miR-200b-3p ZEB2 2.11744072703209 1.66710572104191e-43 -1.60369039498659 1.13296131497065e-43 miRNAWalker2_validate;miRTarBase -0.387016799530245 6.18770056114522e-58 24518294;19787069;24815002;24424572;24769353;19854497;22311119;19544444 This switch involved activation of the transforming growth factor-β TGFβ signaling network that led to a shift in the balance between miR-200 microRNAs and the transcription factor zinc finger E-box-binding homeobox 2 ZEB2 resulting in suppressed transcription of the gene encoding E-cadherin;These findings are surprising since the miR-200 family was previously shown to promote epithelial characteristics by inhibiting the transcriptional repressor Zeb2 and thereby enhancing E-cadherin expression; Expression of miR-200 which promotes a mesenchymal to epithelial cell transition MET by inhibiting Zeb2 expression unexpectedly enhances macroscopic metastases in mouse breast cancer cell lines;Moreover a series of loss-of-function and gain-of-function experiments further indicated that busulfan may have its anti-osteosarcoma effect by upregulating the microRNA-200 miR-200 family which subsequently downregulated its target genes ZEB1 and ZEB2;Furthermore our data suggest that the promoter hypermethylation of ZEB1 and ZEB2 may play an essential role and may overshadow the effects of the miR-200 family in the regulation of EMT during carcinogenesis;Zinc finger E box binding homeobox 2 ZEB2 regulated by miR 200b contributes to multi drug resistance of small cell lung cancer; In silico analysis demonstrated that there are complementary binding sites between miR-200b and ZEB2 3'-UTR and identified miR-200b as a potential regulator of ZEB2; Overexpression of miR-200b led to the downregulation of ZEB2 at protein level; Luciferase reporter gene assay showed that 3'UTR ZEB2 activity was regulated by miR-200b; Our results suggest that ZEB2 modulates drug resistance and is regulated by miR-200b;We used qRT-PCR to examine expression of the miR-200 miRNA family and its predicted targets the ZEB1 and ZEB2 transcriptional repressors in primary cultures of normal cells from the surface of the ovary and in a panel of 70 ovarian cancer tissues and 15 ovarian cancer cell lines; We studied the mechanisms of regulation of miR-200 miRNAs and ZEB transcription factors in ovarian cells using 3' UTR luciferase reporters promoter luciferase reporters and siRNAs miR-200 family members are expressed at low or negligible levels in normal ovarian surface cells and substantially increase in expression in ovarian cancer whereas expression of ZEB1 and ZEB2 shows the opposite pattern;MicroRNA 200b regulates cell proliferation invasion and migration by directly targeting ZEB2 in gastric carcinoma; There was a strong correlation between the levels of miR-200b and ZEB2 and E-cadherin mRNAs in gastric cancer patients; miR-200b regulates ZEB2 expression and thus controls metastasis in gastric cancer;Recent studies have shown that the miR-200 family regulates epithelial-mesenchymal transition EMT by targeting zinc-finger E-box binding homeobox 1 ZEB1 and ZEB2 ;metastasis;;tumorigenesis;drug resistance;;metastasis; breast cancer;breast cancer;sarcoma;head and neck cancer;lung squamous cell cancer;ovarian cancer;gastric cancer;prostate cancer hsa-miR-200c-3p ZEB2 2.07038651806183 6.84613386286599e-51 -1.60369039498659 1.13296131497065e-43 miRNAWalker2_validate;miRTarBase -0.472673002772748 6.29752478367514e-74 24710933;21682933;26935975;25052237;18925646;24885194 miR-200c has been shown to regulate the epithelial-mesenchymal transition EMT by inhibiting ZEB1 and ZEB2 expression in breast cancer cells; This study further examined the role of miR-200c in the invasion and metastasis of breast cancer that goes beyond the regulation on ZEB1 and ZEB2 expression;Of this family miR-200c has garnered particular attention as a consequence of its ability to target ZEB1 and ZEB2 mediators of epithelial- mesenchymal transition;MicroRNA 200c inhibits the metastasis of non small cell lung cancer cells by targeting ZEB2 an epithelial mesenchymal transition regulator;miR 200c modulates ovarian cancer cell metastasis potential by targeting zinc finger E box binding homeobox 2 ZEB2 expression; Luciferase reporter assay confirmed the target of miR-200c as ZEB2; Furthermore miR-200c expression inhibited ovarian cancer cell ES-2 migration and invasion capacity by suppression of ZEB2 expression p < 0.01; Thus targeting of miR-200c or ZEB2 may serve as a potential therapeutic strategy for control of ovarian cancer;We established by quantitative RT-PCR that in CCCs in which miR-141 and miR-200c were down-regulated ZFHX1B a transcriptional repressor for CDH1/E-cadherin tended to be up-regulated; On the basis of these findings we suggest that down-regulation of miR-141 and miR-200c in CCCs might be involved in suppression of CDH1/E-cadherin transcription via up-regulation of ZFHX1B;Two gastric cancer cell lines were treated with IGF-I to induce EMT and levels of transcription factor ZEB2 and microRNA-200c miR-200c were measured; Furthermore both Akt/ERK inhibitors and knockdown of Akt/ERK gene reversed IGF-I-induced ZEB2 up-regulation and EMT through up-regulation of miR-200c suggesting the involvement of an Akt/ERK-miR-200c-ZEB2 axis in IGF-I-induced EMT metastasis;;metastasis;metastasis;; breast cancer;breast cancer;lung squamous cell cancer;ovarian cancer;kidney renal cell cancer;gastric cancer hsa-miR-28-5p ZFHX3 -0.14267073663385 0.0497619246260571 -0.348999974902257 0.000181853915066663 miRanda -0.167550170989698 0.000163066442653836 NA NA NA hsa-miR-28-5p ZFP62 -0.14267073663385 0.0497619246260571 0.325545168406013 2.86196637656788e-07 miRanda -0.13865357278194 4.53335257114054e-06 NA NA NA hsa-miR-141-3p ZFPM2 2.99777540204168 1.26370498493024e-78 -1.48309651759718 5.26430774358294e-23 miRNAWalker2_validate;miRTarBase -0.42417105347186 3.00194018110561e-51 NA NA NA hsa-miR-200a-3p ZFPM2 2.55218480823434 2.96135446816238e-51 -1.48309651759718 5.26430774358294e-23 miRNAWalker2_validate;miRTarBase -0.372246535238814 3.0832686353886e-40 26314828 It was reported that miR-200 can activate PI3K/AKT by targeting FOG2 friend of GATA 2 which directly binds to the p85α regulatory subunit of PI3K lung cancer hsa-miR-200b-3p ZFPM2 2.11744072703209 1.66710572104191e-43 -1.48309651759718 5.26430774358294e-23 miRNAWalker2_validate;miRTarBase -0.40035042800184 1.77712326643107e-37 26314828 It was reported that miR-200 can activate PI3K/AKT by targeting FOG2 friend of GATA 2 which directly binds to the p85α regulatory subunit of PI3K lung cancer hsa-miR-200c-3p ZFPM2 2.07038651806183 6.84613386286599e-51 -1.48309651759718 5.26430774358294e-23 miRNAWalker2_validate;miRTarBase -0.48840691534348 8.2725500362134e-47 NA NA NA hsa-miR-28-5p ZFYVE21 -0.14267073663385 0.0497619246260571 -0.367598845555907 7.77046925532795e-09 miRanda -0.132662159289845 1.30742694588055e-05 NA NA NA hsa-miR-28-5p ZFYVE26 -0.14267073663385 0.0497619246260571 -0.327493136832778 2.35759136357908e-07 miRanda -0.12447247393372 3.86452573331125e-05 NA NA NA hsa-miR-28-5p ZNF141 -0.14267073663385 0.0497619246260571 -0.339631252919259 3.05959108622568e-05 miRanda -0.125874933389015 0.00121089813530106 NA NA NA hsa-miR-132-3p ZNF148 -0.0365213889238607 0.668598301088667 -0.236797617916435 0.000267463091189989 miRNAWalker2_validate -0.104950156605787 6.47112314290448e-05 NA NA NA hsa-miR-28-5p ZNF19 -0.14267073663385 0.0497619246260571 -0.231757613225705 0.0028772583683754 miRanda -0.153894344201966 3.11401369237391e-05 NA NA NA hsa-miR-28-5p ZNF217 -0.14267073663385 0.0497619246260571 0.418864365129461 2.53498724711067e-05 miRanda -0.153414197347742 0.00124263968059985 NA NA NA hsa-miR-28-5p ZNF483 -0.14267073663385 0.0497619246260571 -1.79302155637936 8.09598164766452e-31 miRanda -0.161877451760601 0.0353741074383505 NA NA NA hsa-miR-28-5p ZNF484 -0.14267073663385 0.0497619246260571 -0.394911538640617 4.74176735844624e-05 miRanda -0.157201362671009 0.000693734308711223 NA NA NA hsa-miR-28-5p ZNF584 -0.14267073663385 0.0497619246260571 0.083372296410301 0.142227608562401 miRanda -0.162509824661307 1.23349297245691e-09 NA NA NA hsa-miR-28-5p ZNF609 -0.14267073663385 0.0497619246260571 0.093745834360889 0.193677156970303 miRanda -0.17454071384802 3.08415035241991e-07 NA NA NA hsa-miR-28-5p ZNF615 -0.14267073663385 0.0497619246260571 -0.448908283415558 5.52841871038806e-08 miRanda -0.109992140552008 0.00550144078708249 NA NA NA hsa-miR-28-5p ZNF626 -0.14267073663385 0.0497619246260571 -0.000772536290166048 0.995647761387273 miRanda -0.220256116308172 0.00107200384839584 NA NA NA hsa-miR-132-3p ZNF652 -0.0365213889238607 0.668598301088667 0.213841843999925 0.0221268421718162 miRNAWalker2_validate -0.204923212694746 5.0218733891149e-08 NA NA NA hsa-miR-28-5p ZNF655 -0.14267073663385 0.0497619246260571 -0.550879158758876 1.1977452132855e-09 miRanda -0.10696179348568 0.0141009676103873 NA NA NA hsa-miR-28-5p ZNF665 -0.14267073663385 0.0497619246260571 -0.612934521530682 2.18611918652348e-07 miRanda -0.165068213448631 0.00360053988165736 NA NA NA hsa-miR-28-5p ZNF689 -0.14267073663385 0.0497619246260571 -0.187666926528026 0.172159706978396 miRanda -0.251518404206901 0.000117526224448517 NA NA NA hsa-miR-28-5p ZNF740 -0.14267073663385 0.0497619246260571 -0.0891625358082004 0.165860174035839 miRanda -0.157571961321932 2.19054782197219e-07 NA NA NA hsa-miR-28-5p ZNF764 -0.14267073663385 0.0497619246260571 0.751807638397205 5.3087214361303e-34 miRanda -0.230605997304356 2.28319062007636e-14 NA NA NA hsa-miR-28-5p ZSCAN29 -0.14267073663385 0.0497619246260571 0.00304067676335418 0.961086390220188 miRanda -0.102988503595859 0.000492546003786681 NA NA NA hsa-miR-28-5p ZSWIM5 -0.14267073663385 0.0497619246260571 -0.451593352623076 0.00950484344118356 miRanda -0.585836577748603 9.01191284628358e-13 NA NA NA