miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-181a-5p ATM 2.29556023480783 1.8138457900616e-21 -0.694304374354523 1.11905560067279e-05 miRNAWalker2_validate;miRTarBase;MirTarget -0.126923788457429 2.84207726303723e-05 24531888;27150990;21102523;26113450;23656790 Ataxia-telangiectasia mutation ATM was predicted as a target gene of miR-181a with bioinformatics analysis and was verified by lucifersae reporter assay; A luciferase reporter assay demonstrated that ATM was a direct target of miR-181a miR-181a mimics transfection down regulated ATM mRNA and protein expression; There was inverse correlation between miR-181a and ATM protein expression in gastric cancer and normal gastric tissues; Our study demonstrates that over-expression of miR-181a might be involved in development of gastric cancer by promoting proliferation and inhibiting apoptosis probably through directly targeting ATM miR-181a modulation may be a potential strategy for the development of miRNA-based therapy of gastric cancer;MiR 181a Promotes Proliferation of Human Acute Myeloid Leukemia Cells by Targeting ATM; Dual luciferase reporter gene assay showed that miR-181a significantly suppressed the reporter gene activity containing ATM 3'-UTR by about 56.8% P < 0.05 but it didn't suppress the reporter gene activity containing 3'-UTR ATM mutation; Western blot showed that miR-181a significantly downregulated the expression of ATM in human leukemia cells; It is also found that miR-181a was significantly increased in AML which showed a negative correlation with ATM expression; miR-181a promotes cell proliferation in AML by regulating the tumor suppressor ATM thus it plays the role as oncogene in pathogenesis of AML;Ataxia telangiectasia mutated ATM a target gene of miR-181 exhibited reduced expression in mammospheres and upon TGF-β treatment;miR 181a promotes G1/S transition and cell proliferation in pediatric acute myeloid leukemia by targeting ATM; Pediatric AML patients and healthy controls were enrolled and the expression of miR-181a and ataxia telangiectasia mutated ATM in tissues were examined using quantitative PCR; Moreover cell proliferation and cell cycle were evaluated in several cell lines HL60 NB4 and K562 by using flow cytometry after transfected with miR-181a mimics and inhibitors or ATM siRNA and control siRNA; Finally ATM as the potential target protein of miR-181a was examined; We found that miR-181a was significantly increased in pediatric AML which showed an inverse association with ATM expression; Luciferase activity of the reporter construct identified ATM as the direct molecular target of miR-181a; The results revealed novel mechanism through which miR-181a regulates G1/S transition and cell proliferation in pediatric AML by regulating the tumor suppressor ATM providing insights into the molecular mechanism in pediatric AML;We report that miR-181a and miR-181b were overexpressed in more aggressive breast cancers and their expression correlates inversely with ATM levels ;;;; gastric cancer;acute myeloid leukemia;breast cancer;acute myeloid leukemia;breast cancer hsa-miR-181b-5p ATM 2.49307679431293 3.92829250217627e-20 -0.694304374354523 1.11905560067279e-05 MirTarget -0.106546210489637 8.13310251996423e-05 21102523;23656790 Ataxia telangiectasia mutated ATM a target gene of miR-181 exhibited reduced expression in mammospheres and upon TGF-β treatment;We report that miR-181a and miR-181b were overexpressed in more aggressive breast cancers and their expression correlates inversely with ATM levels ; breast cancer;breast cancer hsa-miR-186-5p ATM 1.47445398888668 3.0781726595413e-15 -0.694304374354523 1.11905560067279e-05 mirMAP -0.162572896698222 4.94897138455636e-05 NA NA NA hsa-miR-18a-5p ATM 3.78535678765353 3.72446596213886e-26 -0.694304374354523 1.11905560067279e-05 miRNAWalker2_validate;miRTarBase;MirTarget -0.132647027735553 2.53006799595493e-10 23437304;25963391;23857602;23229340 MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression colorectal cancer;cervical and endocervical cancer;breast cancer;colon cancer hsa-miR-19b-3p ATM 2.49529833021187 1.57028265846699e-23 -0.694304374354523 1.11905560067279e-05 miRNAWalker2_validate -0.169669050219426 4.03808006538527e-09 NA NA NA hsa-miR-21-5p ATM 2.73773305747971 1.38864982139576e-43 -0.694304374354523 1.11905560067279e-05 mirMAP -0.176310423151013 4.47713388674926e-07 26289851 MiR-21 is an oncomiR that is overexpressed in nearly all cancers including ATC; Hence suppression of miR-21 could pave the way for ATC therapy thyroid cancer hsa-miR-26a-2-3p ATM 0.823520019989974 0.00017092160823769 -0.694304374354523 1.11905560067279e-05 MirTarget -0.118965349103283 0.000759103078575479 NA NA NA hsa-miR-26b-5p ATM 0.885393662798425 3.2116297961629e-06 -0.694304374354523 1.11905560067279e-05 MirTarget -0.112936121726645 0.00532953311791061 NA NA NA hsa-miR-30c-5p ATM 0.778774633108 0.000285599212429136 -0.694304374354523 1.11905560067279e-05 mirMAP -0.107066600206639 0.00288450607513559 NA NA NA hsa-miR-324-5p ATM 2.95683728155095 1.32960514360912e-20 -0.694304374354523 1.11905560067279e-05 miRanda -0.111813212915851 1.04521240946738e-06 NA NA NA hsa-miR-362-3p ATM 2.0756816987665 3.89026064579331e-12 -0.694304374354523 1.11905560067279e-05 miRanda -0.112278812357676 1.35632724301173e-05 NA NA NA hsa-miR-455-5p ATM 2.2582442061694 1.43472128037398e-13 -0.694304374354523 1.11905560067279e-05 miRanda -0.106749319544671 1.15313735793322e-05 NA NA NA hsa-miR-576-5p ATM 2.20467023993664 3.4053475193793e-19 -0.694304374354523 1.11905560067279e-05 mirMAP -0.17622953753732 2.51071157737834e-09 NA NA NA hsa-miR-590-3p ATM 2.59289455139753 1.03200520972022e-21 -0.694304374354523 1.11905560067279e-05 miRanda;mirMAP -0.147925962529486 7.50176131513898e-08 NA NA NA hsa-miR-590-5p ATM 3.18300989862077 2.06813568597893e-26 -0.694304374354523 1.11905560067279e-05 mirMAP -0.155698076358082 1.697182104602e-10 NA NA NA hsa-miR-92a-3p ATM 2.06460102658973 1.44784291084757e-19 -0.694304374354523 1.11905560067279e-05 miRNAWalker2_validate -0.174566583478402 5.02119051710453e-08 NA NA NA hsa-miR-143-3p BBC3 -2.5789192793377 1.55518999614836e-14 1.11623140689651 3.60476730272011e-07 miRNATAP -0.130165583850532 2.4105347480111e-05 NA NA NA hsa-miR-221-3p BBC3 0.451556679024397 0.0909808299917811 1.11623140689651 3.60476730272011e-07 miRNAWalker2_validate;miRTarBase;miRNATAP -0.157029927745573 9.37364503134878e-05 25585941;20813046 MiR 221 induced PUMA silencing mediates immune evasion of bladder cancer cells;MiR 221 and miR 222 target PUMA to induce cell survival in glioblastoma immune evasion;poor survival bladder cancer;glioblastoma hsa-miR-222-3p BBC3 0.851783756239071 0.00266783568486918 1.11623140689651 3.60476730272011e-07 miRNAWalker2_validate;miRNATAP -0.136078291771636 0.000312194570356401 20813046 MiR 221 and miR 222 target PUMA to induce cell survival in glioblastoma poor survival glioblastoma hsa-miR-199a-3p CASP9 0.640600337515787 0.0380326905134914 -0.446359927280876 0.00400976702291555 mirMAP -0.146082132838509 1.00313136531724e-09 23319430 The techniques used were the MTT assay flow cytometry real-time PCR to assess miR-199a expression as also caspase-8 and caspase-9 activity in HepG2 cells treated with Propofol liver cancer hsa-miR-199b-3p CASP9 0.643923492750702 0.0371742251925406 -0.446359927280876 0.00400976702291555 mirMAP -0.146136089499986 9.60609442207656e-10 NA NA NA hsa-miR-222-5p CASP9 1.25632771414778 0.000138653440511922 -0.446359927280876 0.00400976702291555 mirMAP -0.126831808191525 1.31064720813193e-08 NA NA NA hsa-miR-139-5p CCNB1 -1.52606598644743 1.20474151350665e-08 2.5876663861041 1.47856299409004e-23 miRanda -0.228760920591688 2.21107834979824e-06 NA NA NA hsa-miR-23b-3p CCNB2 -0.251132426825047 0.150200321210792 3.44127671644327 6.15726356293966e-31 miRNAWalker2_validate -0.35472868706084 7.95902161675979e-05 NA NA NA hsa-miR-106a-5p CCND1 2.48882002962836 1.99757727374521e-12 0.0828319966762319 0.840332435689448 MirTarget;miRNATAP -0.278915751904438 2.46069960202024e-07 NA NA NA hsa-miR-15b-5p CCND1 1.57112623124278 9.14247846750597e-13 0.0828319966762319 0.840332435689448 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.2743592470705 0.00190316455907807 NA NA NA hsa-miR-186-5p CCND1 1.47445398888668 3.0781726595413e-15 0.0828319966762319 0.840332435689448 mirMAP -0.319993507741074 0.0020448551843367 NA NA NA hsa-miR-193a-3p CCND1 1.60460546928078 2.86169793322984e-07 0.0828319966762319 0.840332435689448 MirTarget;PITA;miRanda -0.351734964348069 1.30056584701733e-08 NA NA NA hsa-miR-195-5p CCND1 -1.44753678070504 8.70594292149271e-09 0.0828319966762319 0.840332435689448 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.232938095379387 0.00278406868271715 21350001;26631043;25823925 Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497 miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer;MiR 195 inhibits the proliferation of human cervical cancer cells by directly targeting cyclin D1; The present study was to evaluate the level of miR-195 and cyclin D1 in CC tissues and cells; We further investigated the molecular mechanisms of miR-195 and cyclin D1 in CC cell lines HeLa and SiHa; Furthermore the expression of miR-195 was inversely proportional to that of cyclin D1 mRNA or protein p = 0.013 p = 0.015 respectively; However the inhibitor of miR-195 promoted the expression of cyclin D1 and cell proliferation; In conclusion our data suggest that miR-195 may have the potential role in treatment of CC patients as well as miR-195 is a novel regulator of invasiveness and tumorigenicity in CC cells by targeting cyclin D1;MicroRNA profiling identifies MiR 195 suppresses osteosarcoma cell metastasis by targeting CCND1; Meanwhile CCND1 was identified as the target gene of miR-195 and further studied; More importantly using real-time PCR we evaluated the expression of miR-195 and CCND1 in osteosarcoma samples from 107 frozen biopsy tissues and 99 formalin- or paraformalin-fixed paraffin-embedded FFPE tissues; Results indicated lowly expressed miR-195 or highly CCND1 correlated with positive overall survival and their expression inversely related to each other; In summary our study suggests miR-195 functions as a tumor metastasis suppressor gene by down-regulating CCND1 and can be used as a potential target in the treatment of osteosarcoma ;;metastasis;poor survival breast cancer;cervical and endocervical cancer;sarcoma hsa-miR-20b-5p CCND1 2.07706563022469 1.10107236083433e-05 0.0828319966762319 0.840332435689448 MirTarget;miRNATAP -0.250774310314038 8.20956147612201e-10 NA NA NA hsa-miR-340-5p CCND1 0.30282999308409 0.157744789519131 0.0828319966762319 0.840332435689448 mirMAP -0.529085780655826 9.29072904979919e-09 NA NA NA hsa-miR-374b-5p CCND1 0.0654362503857184 0.722673188879465 0.0828319966762319 0.840332435689448 miRNAWalker2_validate;MirTarget -0.416222074203323 0.000132219455740898 NA NA NA hsa-miR-497-5p CCND1 0.0257087130056286 0.918145413833358 0.0828319966762319 0.840332435689448 MirTarget;miRNATAP -0.30773442281542 0.000110601843767413 21350001 Raf-1 and Ccnd1 were identified as novel direct targets of miR-195 and miR-497 miR-195/497 expression levels in clinical specimens were found to be correlated inversely with malignancy of breast cancer breast cancer hsa-miR-7-1-3p CCND1 1.84950311431067 1.25300363397654e-13 0.0828319966762319 0.840332435689448 mirMAP -0.247700635127977 0.00138754391814163 NA NA NA hsa-miR-769-3p CCND1 2.19896922202687 5.69250370895053e-12 0.0828319966762319 0.840332435689448 mirMAP -0.231146561837838 0.000210475786569237 NA NA NA hsa-let-7a-3p CCND2 1.42015538786275 7.03375209382654e-13 -2.42747067684236 2.06814828958882e-10 mirMAP -0.440555668621489 2.14967330798537e-06 20418948 MicroRNA let 7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2 prostate cancer hsa-let-7a-5p CCND2 0.618599506841644 2.76973453058748e-05 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.503238938401113 9.92586197811674e-05 20418948 MicroRNA let 7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2 prostate cancer hsa-let-7b-3p CCND2 0.868207752019623 1.30460056774366e-05 -2.42747067684236 2.06814828958882e-10 mirMAP -0.292600423696445 0.00203496813099611 NA NA NA hsa-let-7b-5p CCND2 0.604314269683977 0.00140009936153214 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.372644945722985 0.000202758514128247 NA NA NA hsa-let-7e-5p CCND2 0.860104282510173 6.04758298373779e-05 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.254279031336757 0.00390630067355032 NA NA NA hsa-let-7f-1-3p CCND2 1.55054488387679 7.31326775537952e-11 -2.42747067684236 2.06814828958882e-10 mirMAP -0.349075249109793 1.19776506071246e-05 NA NA NA hsa-let-7f-5p CCND2 0.495648727272215 0.00355972741198794 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.395067035409999 0.000431247614557398 NA NA NA hsa-let-7g-5p CCND2 1.20061455355116 1.50237505511483e-12 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.78482657734201 2.2934101130382e-13 NA NA NA hsa-miR-106a-5p CCND2 2.48882002962836 1.99757727374521e-12 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.460265785003224 1.90572227650597e-20 NA NA NA hsa-miR-106b-5p CCND2 2.46782146831112 1.36171495805632e-23 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.723802321113981 4.62852843641777e-26 NA NA NA hsa-miR-10b-3p CCND2 0.77597971642517 0.00264877454133753 -2.42747067684236 2.06814828958882e-10 mirMAP -0.299055149155965 4.03151748542262e-05 NA NA NA hsa-miR-130b-5p CCND2 2.9963110846538 1.98883029416455e-18 -2.42747067684236 2.06814828958882e-10 mirMAP -0.524276006004661 2.15252650149472e-24 NA NA NA hsa-miR-141-3p CCND2 5.02396127982626 2.19224509139725e-24 -2.42747067684236 2.06814828958882e-10 MirTarget;TargetScan -0.336824974322951 8.46453268716016e-23 NA NA NA hsa-miR-151a-3p CCND2 1.24018761527407 1.08839919675408e-07 -2.42747067684236 2.06814828958882e-10 mirMAP -0.618872158164273 1.57062556462901e-15 NA NA NA hsa-miR-15a-5p CCND2 2.34669284378787 1.69544424500258e-23 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.558385276567589 5.78612617845985e-14 NA NA NA hsa-miR-15b-5p CCND2 1.57112623124278 9.14247846750597e-13 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.814047353716774 1.36644748676197e-24 NA NA NA hsa-miR-16-2-3p CCND2 2.31623086727196 4.29838149388188e-20 -2.42747067684236 2.06814828958882e-10 mirMAP -0.524415153934827 5.42085565840902e-14 NA NA NA hsa-miR-16-5p CCND2 1.75824877145857 1.68592440033108e-16 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRNATAP -0.624880555696101 7.5948593080299e-14 NA NA NA hsa-miR-17-5p CCND2 3.2710598467455 9.93128340539455e-27 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.576586035201243 5.39762569803648e-26 NA NA NA hsa-miR-181a-2-3p CCND2 2.19062681262366 3.81790102612782e-13 -2.42747067684236 2.06814828958882e-10 mirMAP -0.371385706331271 5.48097191184985e-10 NA NA NA hsa-miR-182-5p CCND2 3.53923166221814 5.07781258121574e-21 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.418107303561313 5.83833309647385e-20 NA NA NA hsa-miR-183-5p CCND2 4.19757025992371 4.79612296318304e-26 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.406097602561121 1.1832385157764e-21 NA NA NA hsa-miR-185-5p CCND2 2.34016206907488 1.17481626642022e-26 -2.42747067684236 2.06814828958882e-10 MirTarget;miRNATAP -0.596214855521239 5.09732672184216e-14 NA NA NA hsa-miR-186-5p CCND2 1.47445398888668 3.0781726595413e-15 -2.42747067684236 2.06814828958882e-10 mirMAP;miRNATAP -0.753228825405404 3.55444659312987e-15 NA NA NA hsa-miR-188-5p CCND2 2.52825126596644 6.78099435999778e-14 -2.42747067684236 2.06814828958882e-10 PITA;mirMAP -0.327199573697285 9.78244941189197e-09 NA NA NA hsa-miR-191-5p CCND2 2.30038190670664 9.93191704545938e-17 -2.42747067684236 2.06814828958882e-10 MirTarget -0.63056469865633 4.58280529094783e-24 NA NA NA hsa-miR-19a-3p CCND2 3.42309334186656 3.02671914353412e-28 -2.42747067684236 2.06814828958882e-10 MirTarget;miRNATAP -0.478555105133488 2.03415225430867e-18 NA NA NA hsa-miR-19b-3p CCND2 2.49529833021187 1.57028265846699e-23 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;MirTarget;miRNATAP -0.644240893236217 5.93065810659583e-21 NA NA NA hsa-miR-200a-3p CCND2 4.59434485520731 6.38952518573476e-18 -2.42747067684236 2.06814828958882e-10 MirTarget -0.322639326506763 1.12744821991113e-23 NA NA NA hsa-miR-20a-5p CCND2 3.15577933619191 4.19753346442454e-25 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;miRTarBase;miRNATAP -0.552675029838617 1.98133209896024e-23 NA NA NA hsa-miR-20b-5p CCND2 2.07706563022469 1.10107236083433e-05 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.296015277168294 1.16933542500196e-14 NA NA NA hsa-miR-21-3p CCND2 2.19296355304364 4.63665722759779e-15 -2.42747067684236 2.06814828958882e-10 mirMAP -0.266420777851125 4.25531839718567e-05 NA NA NA hsa-miR-224-3p CCND2 1.87536386559317 6.15140969394846e-05 -2.42747067684236 2.06814828958882e-10 mirMAP -0.234939524450716 1.27567539540633e-08 NA NA NA hsa-miR-26b-5p CCND2 0.885393662798425 3.2116297961629e-06 -2.42747067684236 2.06814828958882e-10 mirMAP;miRNATAP -0.333191234288059 0.000785967313666436 NA NA NA hsa-miR-29b-3p CCND2 1.65828197923813 1.02676780888141e-09 -2.42747067684236 2.06814828958882e-10 MirTarget;miRNATAP -0.181452610770389 0.00800883535071036 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-301a-3p CCND2 2.81024345929638 2.29784847962242e-17 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate -0.490714440601798 1.13987935585442e-18 NA NA NA hsa-miR-3065-3p CCND2 2.16221780121195 9.65525739884541e-09 -2.42747067684236 2.06814828958882e-10 MirTarget;miRNATAP -0.234083951058536 1.60505150894369e-06 NA NA NA hsa-miR-3065-5p CCND2 2.74629919868982 1.86846589298284e-08 -2.42747067684236 2.06814828958882e-10 mirMAP -0.236930159170796 2.73973057309239e-07 NA NA NA hsa-miR-30d-3p CCND2 0.977747550180601 4.3048212205381e-05 -2.42747067684236 2.06814828958882e-10 mirMAP -0.514799428246913 2.69094766335815e-11 NA NA NA hsa-miR-30e-3p CCND2 -0.215663037684509 0.235375122783546 -2.42747067684236 2.06814828958882e-10 mirMAP -0.317748716556734 0.00256884077064715 NA NA NA hsa-miR-32-3p CCND2 2.02436999565685 5.94091197945307e-09 -2.42747067684236 2.06814828958882e-10 mirMAP -0.37065809204682 5.74913234109809e-10 NA NA NA hsa-miR-320a CCND2 0.438165828017084 0.0390159073347133 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate;mirMAP;miRNATAP -0.366959333753783 3.90012764364419e-05 NA NA NA hsa-miR-320b CCND2 1.55692969039548 1.41071034251915e-08 -2.42747067684236 2.06814828958882e-10 mirMAP;miRNATAP -0.389495451202309 5.62361965191887e-09 NA NA NA hsa-miR-324-3p CCND2 2.08739139768981 3.11885987067952e-13 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate -0.569926030100518 1.57078270718816e-20 NA NA NA hsa-miR-331-5p CCND2 2.17414714166315 1.54547681391888e-18 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.55418360999634 4.24751797285043e-14 NA NA NA hsa-miR-335-5p CCND2 1.77247705492746 2.22457658872091e-06 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate -0.196309653133352 7.59470226154717e-05 NA NA NA hsa-miR-33a-3p CCND2 1.72765312072219 1.51784443453521e-07 -2.42747067684236 2.06814828958882e-10 MirTarget -0.474892003832155 3.60158523540778e-15 NA NA NA hsa-miR-3607-3p CCND2 2.68532138541128 5.66716351955047e-14 -2.42747067684236 2.06814828958882e-10 mirMAP -0.253991708738003 1.1264040881505e-06 NA NA NA hsa-miR-374b-3p CCND2 0.930487774873495 2.74420094310516e-05 -2.42747067684236 2.06814828958882e-10 mirMAP -0.379294826109076 7.61411367904561e-06 NA NA NA hsa-miR-378a-3p CCND2 -0.10995720000828 0.716188270032681 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate -0.264766627168263 2.14263562743039e-05 NA NA NA hsa-miR-423-5p CCND2 0.963563145758616 9.86109015204915e-07 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate -0.681308347713645 2.78087673863117e-13 NA NA NA hsa-miR-424-5p CCND2 1.61930224569578 1.89642549274994e-10 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.208747216207423 0.00424259734488207 NA NA NA hsa-miR-429 CCND2 4.48815175680773 2.44117492218206e-17 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.316889169500172 1.77696184266368e-22 NA NA NA hsa-miR-450b-5p CCND2 1.69244418548325 1.68064054148881e-10 -2.42747067684236 2.06814828958882e-10 MirTarget;PITA;miRNATAP -0.299286922863212 1.69011727706147e-05 NA NA NA hsa-miR-500a-5p CCND2 1.72110365584658 3.0807246212659e-10 -2.42747067684236 2.06814828958882e-10 mirMAP -0.230284164405623 0.000679591942778451 NA NA NA hsa-miR-501-5p CCND2 1.93654809410041 1.3779381780759e-10 -2.42747067684236 2.06814828958882e-10 PITA;mirMAP;miRNATAP -0.278516160908165 9.05358509837179e-06 NA NA NA hsa-miR-503-5p CCND2 3.66513190181895 6.60300917645278e-21 -2.42747067684236 2.06814828958882e-10 MirTarget -0.31264390956225 5.2815091282227e-11 25860935 We then identified two targets of miR-503 CCND2 and CCND3 breast cancer hsa-miR-550a-5p CCND2 2.11025227932763 6.94489556637146e-12 -2.42747067684236 2.06814828958882e-10 MirTarget -0.44540662591501 2.41152035101861e-14 NA NA NA hsa-miR-589-3p CCND2 2.51971841868825 2.39125940264107e-11 -2.42747067684236 2.06814828958882e-10 mirMAP -0.360588568041429 3.76015378643146e-11 NA NA NA hsa-miR-590-3p CCND2 2.59289455139753 1.03200520972022e-21 -2.42747067684236 2.06814828958882e-10 miRanda;mirMAP -0.419988530136573 3.79848108645639e-10 NA NA NA hsa-miR-590-5p CCND2 3.18300989862077 2.06813568597893e-26 -2.42747067684236 2.06814828958882e-10 mirMAP -0.50562344923364 6.73026539304424e-18 NA NA NA hsa-miR-660-5p CCND2 1.82692095272076 1.92040083607196e-13 -2.42747067684236 2.06814828958882e-10 mirMAP -0.361636929774302 8.5078492819456e-07 NA NA NA hsa-miR-7-1-3p CCND2 1.84950311431067 1.25300363397654e-13 -2.42747067684236 2.06814828958882e-10 mirMAP -0.518854235917429 5.32697612805784e-13 NA NA NA hsa-miR-708-3p CCND2 2.80907765282502 3.07460722669281e-14 -2.42747067684236 2.06814828958882e-10 mirMAP -0.177604972093223 0.000316375213374722 NA NA NA hsa-miR-708-5p CCND2 3.38051631555486 5.12590198690777e-18 -2.42747067684236 2.06814828958882e-10 mirMAP -0.19367896307831 2.62089031491477e-05 NA NA NA hsa-miR-877-5p CCND2 2.94916202082736 1.8151927276044e-13 -2.42747067684236 2.06814828958882e-10 miRNAWalker2_validate -0.361363420346396 6.83899428438154e-13 NA NA NA hsa-miR-93-5p CCND2 3.03961860136032 1.11672209617395e-24 -2.42747067684236 2.06814828958882e-10 miRNATAP -0.640135233296746 3.73391346199191e-30 NA NA NA hsa-miR-944 CCND2 2.91186649218281 1.31574753528144e-06 -2.42747067684236 2.06814828958882e-10 PITA;mirMAP -0.168575408637895 9.32931241492905e-08 NA NA NA hsa-miR-96-5p CCND2 4.89433085814374 1.92000659886961e-31 -2.42747067684236 2.06814828958882e-10 TargetScan;miRNATAP -0.382951897231727 7.25785958201884e-21 NA NA NA hsa-miR-27b-3p CCND3 0.196007704287769 0.295630157035388 0.142518749044219 0.397307080616203 miRNAWalker2_validate -0.133645004740845 0.00231237621077374 NA NA NA hsa-miR-28-3p CCNE2 -0.411384408160536 0.0101470262574251 2.48639159684199 1.03709868150437e-14 PITA;miRNATAP -0.310525624419087 0.00235062649082895 NA NA NA hsa-miR-100-5p CCNG1 -1.48442967087177 1.5610956769298e-05 -0.630675935972558 0.000400486995429734 miRNAWalker2_validate -0.105342313079245 2.17217874265015e-05 NA NA NA hsa-miR-142-3p CCNG1 2.75247908790621 1.45636065474764e-12 -0.630675935972558 0.000400486995429734 miRanda -0.126463547200282 2.83099670815953e-09 NA NA NA hsa-miR-142-5p CCNG1 1.17581605694506 0.0018174701991234 -0.630675935972558 0.000400486995429734 mirMAP -0.135444917988823 1.64976499538214e-09 NA NA NA hsa-miR-146b-5p CCNG1 1.75587235896724 1.15724631266659e-07 -0.630675935972558 0.000400486995429734 miRanda -0.191979568311146 1.57873329842222e-14 NA NA NA hsa-miR-181a-5p CCNG1 2.29556023480783 1.8138457900616e-21 -0.630675935972558 0.000400486995429734 miRNAWalker2_validate -0.138300938705756 4.97778883789593e-05 NA NA NA hsa-miR-21-5p CCNG1 2.73773305747971 1.38864982139576e-43 -0.630675935972558 0.000400486995429734 miRNAWalker2_validate -0.334984360503205 1.41305076025776e-18 NA NA NA hsa-miR-212-3p CCNG1 0.357878936470387 0.232446214501331 -0.630675935972558 0.000400486995429734 MirTarget -0.123895323476886 1.87466670079056e-05 NA NA NA hsa-miR-23a-3p CCNG1 1.00493340843378 2.55313889288924e-08 -0.630675935972558 0.000400486995429734 MirTarget;miRNATAP -0.235176230198438 6.74971203243017e-07 NA NA NA hsa-miR-24-3p CCNG1 0.99989036772428 1.35595013650376e-07 -0.630675935972558 0.000400486995429734 miRNAWalker2_validate -0.169125446700509 0.000190004265954157 NA NA NA hsa-miR-27a-3p CCNG1 1.66542180327138 4.93775737894805e-13 -0.630675935972558 0.000400486995429734 MirTarget;miRNATAP -0.273948809034678 1.47372649130546e-14 NA NA NA hsa-miR-299-5p CCNG1 -0.274550626427337 0.386834031856446 -0.630675935972558 0.000400486995429734 MirTarget -0.121890819304204 7.68802944015312e-06 NA NA NA hsa-miR-409-3p CCNG1 0.877551657794358 0.00171210686733898 -0.630675935972558 0.000400486995429734 MirTarget;PITA;miRNATAP -0.125912635528632 4.1602286804454e-05 NA NA NA hsa-let-7a-2-3p CCNG2 0.0400317820791161 0.91528727576685 0.294593163175018 0.224850601777415 MirTarget -0.188300071549428 1.469681835993e-09 NA NA NA hsa-let-7f-1-3p CCNG2 1.55054488387679 7.31326775537952e-11 0.294593163175018 0.224850601777415 MirTarget;mirMAP -0.17426620141391 0.000452710207736509 NA NA NA hsa-miR-1271-5p CCNG2 0.970418832993172 0.000609742584380688 0.294593163175018 0.224850601777415 MirTarget -0.135322770237981 0.00106266066652335 NA NA NA hsa-miR-142-5p CCNG2 1.17581605694506 0.0018174701991234 0.294593163175018 0.224850601777415 MirTarget;miRNATAP -0.176085484773735 7.75837129278938e-09 NA NA NA hsa-miR-146b-5p CCNG2 1.75587235896724 1.15724631266659e-07 0.294593163175018 0.224850601777415 miRNATAP -0.27020803241081 1.23701917372016e-15 NA NA NA hsa-miR-193a-3p CCNG2 1.60460546928078 2.86169793322984e-07 0.294593163175018 0.224850601777415 miRanda -0.129094145694519 0.000475999179918851 NA NA NA hsa-miR-21-5p CCNG2 2.73773305747971 1.38864982139576e-43 0.294593163175018 0.224850601777415 mirMAP -0.174747091120676 0.00112665544893574 NA NA NA hsa-miR-29a-5p CCNG2 1.46749674755271 3.34605004270294e-07 0.294593163175018 0.224850601777415 mirMAP -0.206679112326703 1.00351662471714e-06 NA NA NA hsa-miR-340-5p CCNG2 0.30282999308409 0.157744789519131 0.294593163175018 0.224850601777415 MirTarget;mirMAP -0.205624430662132 0.000184435874836499 27374211 MicroRNA 340 promotes the tumor growth of human gastric cancer by inhibiting cyclin G2; Notably alteration of miR-340 expression affected the luciferase activity of wild-type 3'-UTR of cyclin G2 CCNG2 and regulated CCNG2 abundance in gastric cancer cells indicating that CCNG2 is a direct target of miR-340; Moreover CCNG2 knockdown eradicated the effects of miR-340 silencing on gastric cancer cells gastric cancer hsa-miR-342-3p CCNG2 1.49115144672245 1.43879249744621e-07 0.294593163175018 0.224850601777415 miRanda -0.146971916288753 0.000310344981815528 NA NA NA hsa-miR-485-3p CCNG2 0.442820096243914 0.166634696770945 0.294593163175018 0.224850601777415 MirTarget;mirMAP -0.115099732985913 0.00184797783339564 NA NA NA hsa-miR-655-3p CCNG2 0.485339318272148 0.137014658258732 0.294593163175018 0.224850601777415 MirTarget -0.124045178250952 0.000684463971107448 NA NA NA hsa-miR-129-5p CD82 -0.524170159980738 0.222582956006346 -0.284703364892811 0.431093109415599 miRanda -0.176694707949068 1.79871640989954e-05 NA NA NA hsa-miR-491-5p CD82 0.615770352820862 0.0418295628761654 -0.284703364892811 0.431093109415599 miRanda -0.286441087121347 3.21716462822837e-06 NA NA NA hsa-miR-101-3p CDK6 0.522819989585461 0.00375627731618038 -0.774202349805119 0.064787030605636 mirMAP -0.728162460077577 6.25283110395347e-11 NA NA NA hsa-miR-103a-3p CDK6 1.44083190496858 1.53799939616416e-13 -0.774202349805119 0.064787030605636 miRNAWalker2_validate;miRNATAP -0.738308002788887 9.81610683337994e-14 NA NA NA hsa-miR-106a-5p CDK6 2.48882002962836 1.99757727374521e-12 -0.774202349805119 0.064787030605636 mirMAP -0.23817017599031 1.76828696872453e-05 NA NA NA hsa-miR-106b-5p CDK6 2.46782146831112 1.36171495805632e-23 -0.774202349805119 0.064787030605636 mirMAP -0.506703206296417 3.6460930000884e-11 NA NA NA hsa-miR-107 CDK6 1.30977326593435 1.30372468603965e-11 -0.774202349805119 0.064787030605636 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.535825292120497 1.58523365512466e-07 19407485;22491216;21264532;19688090 Enforced expression of miR-107 in MiaPACA-2 and PANC-1 cells downregulated in vitro growth and this was associated with repression of the putative miR-107 target cyclin-dependent kinase 6 thereby providing a functional basis for the epigenetic inactivation of this miRNA in pancreatic cancer;Levels of known miR-107 targets protein kinase Cε PKCε cyclin-dependent kinase 6 CDK6 and hypoxia-inducible factor 1-β HIF1-β decreased following NP/pre-miR-107 treatment;We have identified miR-107 as a potential regulator of CDK6 expression; A bioinformatics search revealed a putative target site for miR-107 within the CDK6 3' untranslated region; Expression of miR-107 in gastric cancer cell lines was found inversely correlated with CDK6 expression; miR-107 could significantly suppress CDK6 3' UTR luciferase reporter activity and this effect was not detectable when the putative 3' UTR target site was mutated; Consistent with the results of the reporter assay ectopic expression of miR-107 reduced both mRNA and protein expression levels of CDK6 inhibited proliferation induced G1 cell cycle arrest and blocked invasion of the gastric cancer cells; Our results suggest that miR-107 may have a tumor suppressor function by directly targeting CDK6 to inhibit the proliferation and invasion activities of gastric cancer cells;Using miRNA-target prediction analyses and the array data we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6 ;;; pancreatic cancer;head and neck cancer;gastric cancer;lung squamous cell cancer hsa-miR-141-3p CDK6 5.02396127982626 2.19224509139725e-24 -0.774202349805119 0.064787030605636 TargetScan;miRNATAP -0.280860480145549 9.29496321423746e-14 NA NA NA hsa-miR-148b-3p CDK6 1.97815018171325 6.07715327281595e-26 -0.774202349805119 0.064787030605636 mirMAP -0.689303354555952 6.39464837566244e-12 NA NA NA hsa-miR-148b-5p CDK6 2.18024067300781 2.84770452413262e-14 -0.774202349805119 0.064787030605636 mirMAP -0.337506526013873 2.86415828815712e-06 NA NA NA hsa-miR-15a-5p CDK6 2.34669284378787 1.69544424500258e-23 -0.774202349805119 0.064787030605636 miRNATAP -0.331153083238962 5.09100262040034e-05 NA NA NA hsa-miR-16-1-3p CDK6 2.56983217111073 3.02262181237943e-20 -0.774202349805119 0.064787030605636 mirMAP -0.214448709710321 0.00270430071750274 NA NA NA hsa-miR-17-5p CDK6 3.2710598467455 9.93128340539455e-27 -0.774202349805119 0.064787030605636 TargetScan;mirMAP -0.168193833076577 0.00702167500067962 NA NA NA hsa-miR-182-5p CDK6 3.53923166221814 5.07781258121574e-21 -0.774202349805119 0.064787030605636 mirMAP -0.342809938093979 8.28135113019132e-12 NA NA NA hsa-miR-185-5p CDK6 2.34016206907488 1.17481626642022e-26 -0.774202349805119 0.064787030605636 miRNAWalker2_validate -0.418001590004214 1.44577313582161e-06 19688090 Using miRNA-target prediction analyses and the array data we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6 lung squamous cell cancer hsa-miR-191-5p CDK6 2.30038190670664 9.93191704545938e-17 -0.774202349805119 0.064787030605636 miRNAWalker2_validate;miRTarBase -0.711524835034044 1.00464473356187e-26 NA NA NA hsa-miR-200a-3p CDK6 4.59434485520731 6.38952518573476e-18 -0.774202349805119 0.064787030605636 miRNATAP -0.336691687165474 2.80396820395637e-22 24009066 microRNA 200a is an independent prognostic factor of hepatocellular carcinoma and induces cell cycle arrest by targeting CDK6 liver cancer hsa-miR-200b-3p CDK6 3.78235093249346 3.18343003239465e-14 -0.774202349805119 0.064787030605636 mirMAP -0.361406013032565 5.1342063993974e-22 NA NA NA hsa-miR-200c-3p CDK6 3.50132673580082 2.42716349086937e-15 -0.774202349805119 0.064787030605636 mirMAP -0.320385878451071 9.82419842455136e-14 NA NA NA hsa-miR-20a-5p CDK6 3.15577933619191 4.19753346442454e-25 -0.774202349805119 0.064787030605636 mirMAP -0.188851341814342 0.00261077138275469 NA NA NA hsa-miR-20b-5p CDK6 2.07706563022469 1.10107236083433e-05 -0.774202349805119 0.064787030605636 mirMAP -0.293918462449454 1.28039252847365e-12 26166554 The transfection of miR-20b into EJ cells induced G1 phase cell cycle arrest via the decreased expression of cyclin D1 CDK2 and CDK6 without affecting another G1 phase cell cycle regulator cyclin E bladder cancer hsa-miR-29b-3p CDK6 1.65828197923813 1.02676780888141e-09 -0.774202349805119 0.064787030605636 miRNAWalker2_validate;miRTarBase;miRNATAP -0.224461155748557 0.0022317971289129 23245396;25472644;26180082;23591808;27230400;20086245 The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6 which we proved to be a direct target of miR-29 in these cells;Moreover miR-29b inhibited the expression of MCL1 and CDK6;Knockdown of NTSR1 increased the expression of miR-29b-1 and miR-129-3p which were responsible for the decreased CDK6 expression;Here we have identified the oncogene cyclin-dependent protein kinase 6 CDK6 as a direct target of miR-29b in lung cancer;MiR 29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6; In this study we investigated the role of miR-29b as a novel regulator of CDK6 using bioinformatics methods; We demonstrated that CDK6 can be downregulated by miR-29b via binding to the 3'-UTR region in osteosarcoma cells; Furthermore we identified an inverse correlation between miR-29b and CDK6 protein levels in osteosarcoma tissues; The results revealed that miR-29b acts as a tumor suppressor of osteosarcoma by targeting CDK6 in the proliferation and migration processes;microRNA expression profile and identification of miR 29 as a prognostic marker and pathogenetic factor by targeting CDK6 in mantle cell lymphoma; Furthermore we demonstrate miR-29 inhibition of CDK6 protein and mRNA levels by direct binding to 3'-untranslated region; Inverse correlation between miR-29 and CDK6 was observed in MCL ;;;;; melanoma;colorectal cancer;glioblastoma;lung cancer;sarcoma;lymphoma hsa-miR-29c-3p CDK6 -0.00968972577705429 0.97099586346872 -0.774202349805119 0.064787030605636 miRNAWalker2_validate;miRTarBase;miRNATAP -0.580639861705711 5.15109801814716e-15 26396669 Furthermore through qPCR and Western blot assays confirmed that overexpression of miR-29c reduced CDK6 mRNA and protein levels; miR-29c could inhibit the proliferation migration and invasion of bladder cancer cells via regulating CDK6 bladder cancer hsa-miR-30b-5p CDK6 0.800822374912712 0.000133601914525413 -0.774202349805119 0.064787030605636 mirMAP -0.540189292799148 1.61932566833175e-08 NA NA NA hsa-miR-30c-5p CDK6 0.778774633108 0.000285599212429136 -0.774202349805119 0.064787030605636 mirMAP -0.727075100437403 2.31302505595533e-15 NA NA NA hsa-miR-30d-3p CDK6 0.977747550180601 4.3048212205381e-05 -0.774202349805119 0.064787030605636 mirMAP -0.561334088800932 1.30656981501796e-11 NA NA NA hsa-miR-30d-5p CDK6 0.675423997639582 0.00270618816039773 -0.774202349805119 0.064787030605636 mirMAP -0.785477623920694 1.19356262091604e-19 NA NA NA hsa-miR-30e-3p CDK6 -0.215663037684509 0.235375122783546 -0.774202349805119 0.064787030605636 mirMAP -0.686700064203411 6.84849442105303e-10 NA NA NA hsa-miR-30e-5p CDK6 1.23904909539663 1.35561917122157e-09 -0.774202349805119 0.064787030605636 mirMAP -0.817058326987529 5.2385475320723e-18 NA NA NA hsa-miR-32-3p CDK6 2.02436999565685 5.94091197945307e-09 -0.774202349805119 0.064787030605636 mirMAP -0.249929478125797 0.000125097299153361 NA NA NA hsa-miR-32-5p CDK6 2.34056473219422 6.24134585974322e-20 -0.774202349805119 0.064787030605636 miRNATAP -0.295032649435311 9.55907730341779e-05 NA NA NA hsa-miR-320a CDK6 0.438165828017084 0.0390159073347133 -0.774202349805119 0.064787030605636 PITA;miRNATAP -0.376224025256019 8.78451776886182e-05 NA NA NA hsa-miR-330-3p CDK6 0.876747334239085 0.00073709016089703 -0.774202349805119 0.064787030605636 PITA;mirMAP -0.427895514362065 2.76679372346252e-08 NA NA NA hsa-miR-335-3p CDK6 3.08791651139852 5.31309869723517e-12 -0.774202349805119 0.064787030605636 mirMAP -0.153251191165497 0.000493500059209 NA NA NA hsa-miR-335-5p CDK6 1.77247705492746 2.22457658872091e-06 -0.774202349805119 0.064787030605636 mirMAP -0.166621089394833 0.00183832978506415 NA NA NA hsa-miR-33a-5p CDK6 3.79671563098419 1.15966309820345e-18 -0.774202349805119 0.064787030605636 miRNATAP -0.187324589024782 0.000129271884100387 NA NA NA hsa-miR-34a-3p CDK6 1.75590866103657 8.02801510630657e-09 -0.774202349805119 0.064787030605636 mirMAP -0.376950684687892 1.73963312821001e-07 21702042;26104764 Molecular analyses identified Cdk6 and sirtuin SIRT-1 as being targeted by miR-34a in MI-TCC cells however inhibition of Cdk6 and SIRT-1 was not as effective as pre-miR-34a in mediating chemosensitization;The expression of microRNA 34a is inversely correlated with c MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients; We found significant inverse correlations between miR-34a and c-MET R = -0.316 P = 0.028 and CDK6 expression R = -0.4582 P = 0.004 ; bladder cancer;lung cancer hsa-miR-34a-5p CDK6 1.9016323452565 1.43092834643601e-14 -0.774202349805119 0.064787030605636 miRNAWalker2_validate;miRTarBase;miRNATAP -0.519263514540718 3.07288169543583e-11 21702042;26104764 Molecular analyses identified Cdk6 and sirtuin SIRT-1 as being targeted by miR-34a in MI-TCC cells however inhibition of Cdk6 and SIRT-1 was not as effective as pre-miR-34a in mediating chemosensitization;The expression of microRNA 34a is inversely correlated with c MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients; We found significant inverse correlations between miR-34a and c-MET R = -0.316 P = 0.028 and CDK6 expression R = -0.4582 P = 0.004 ; bladder cancer;lung cancer hsa-miR-3613-5p CDK6 2.64358387329263 1.0373753017245e-24 -0.774202349805119 0.064787030605636 miRNATAP -0.21181572504448 0.005712534663484 NA NA NA hsa-miR-362-5p CDK6 1.59303749908759 9.582675908797e-08 -0.774202349805119 0.064787030605636 mirMAP -0.313855526332272 2.37748256302272e-06 NA NA NA hsa-miR-378a-3p CDK6 -0.10995720000828 0.716188270032681 -0.774202349805119 0.064787030605636 mirMAP -0.397192555694478 2.01427891085214e-09 23333942 Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378 respectively gastric cancer hsa-miR-425-5p CDK6 3.07071373939774 4.02947460586085e-25 -0.774202349805119 0.064787030605636 mirMAP -0.484163394229156 1.07269848409271e-14 NA NA NA hsa-miR-429 CDK6 4.48815175680773 2.44117492218206e-17 -0.774202349805119 0.064787030605636 mirMAP;miRNATAP -0.337479964584767 4.50509176972113e-22 NA NA NA hsa-miR-450b-5p CDK6 1.69244418548325 1.68064054148881e-10 -0.774202349805119 0.064787030605636 mirMAP -0.201384976718992 0.00743415077141657 NA NA NA hsa-miR-497-5p CDK6 0.0257087130056286 0.918145413833358 -0.774202349805119 0.064787030605636 miRNATAP -0.245332138327252 0.00264647539053154 NA NA NA hsa-miR-500a-5p CDK6 1.72110365584658 3.0807246212659e-10 -0.774202349805119 0.064787030605636 mirMAP -0.232725295393576 0.00140479513304275 NA NA NA hsa-miR-501-3p CDK6 1.83132758513743 1.10971547702065e-10 -0.774202349805119 0.064787030605636 PITA;TargetScan;miRNATAP -0.414303056979818 1.84547250783893e-09 NA NA NA hsa-miR-502-3p CDK6 1.50560877085049 1.2647489756373e-11 -0.774202349805119 0.064787030605636 PITA;miRNATAP -0.4275399463222 1.5008131463888e-06 NA NA NA hsa-miR-590-5p CDK6 3.18300989862077 2.06813568597893e-26 -0.774202349805119 0.064787030605636 mirMAP;miRNATAP -0.201344503599928 0.00209551899019727 NA NA NA hsa-miR-592 CDK6 1.51765968871077 0.000636335248013204 -0.774202349805119 0.064787030605636 mirMAP -0.143284258807414 0.0019385912538056 NA NA NA hsa-miR-660-5p CDK6 1.82692095272076 1.92040083607196e-13 -0.774202349805119 0.064787030605636 mirMAP -0.339962414215247 1.75914031108584e-05 NA NA NA hsa-miR-664a-3p CDK6 0.634721925861133 0.00520070178640421 -0.774202349805119 0.064787030605636 mirMAP -0.700489354678287 6.77094321174664e-16 NA NA NA hsa-miR-671-5p CDK6 2.97733652243246 1.21148452344738e-23 -0.774202349805119 0.064787030605636 PITA;miRNATAP -0.255214929025129 0.000181908946141654 NA NA NA hsa-miR-92a-3p CDK6 2.06460102658973 1.44784291084757e-19 -0.774202349805119 0.064787030605636 miRNATAP -0.240406113182612 0.00492937850760914 NA NA NA hsa-miR-93-5p CDK6 3.03961860136032 1.11672209617395e-24 -0.774202349805119 0.064787030605636 mirMAP -0.467996685199197 1.00999249823312e-13 NA NA NA hsa-let-7d-5p CDKN1A 1.33236798905207 1.1741085658466e-13 -1.29236263370998 3.21238784768704e-06 MirTarget -0.324235774348598 1.11339571601414e-05 NA NA NA hsa-let-7f-5p CDKN1A 0.495648727272215 0.00355972741198794 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;MirTarget -0.24004761105408 0.00296779895445168 NA NA NA hsa-let-7g-5p CDKN1A 1.20061455355116 1.50237505511483e-12 -1.29236263370998 3.21238784768704e-06 MirTarget -0.261596530769954 0.000909666186900533 NA NA NA hsa-miR-101-3p CDKN1A 0.522819989585461 0.00375627731618038 -1.29236263370998 3.21238784768704e-06 MirTarget -0.196450098291035 0.00981579805828264 NA NA NA hsa-miR-106a-5p CDKN1A 2.48882002962836 1.99757727374521e-12 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.128693729012364 0.000550088861161385 25510666 After prediction with online software we further used dual-luciferase reporter gene assay to ensure that TP53INP1 and CDKN1A might be the direct targets of miR-106a liver cancer hsa-miR-106b-5p CDKN1A 2.46782146831112 1.36171495805632e-23 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.301959313712314 4.48472102839421e-09 NA NA NA hsa-miR-17-5p CDKN1A 3.2710598467455 9.93128340539455e-27 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.172861511846616 3.07251784468708e-05 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-182-5p CDKN1A 3.53923166221814 5.07781258121574e-21 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate -0.109364926293111 0.00141758512280426 NA NA NA hsa-miR-20a-5p CDKN1A 3.15577933619191 4.19753346442454e-25 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.165884225022855 7.19614215488726e-05 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-20b-5p CDKN1A 2.07706563022469 1.10107236083433e-05 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.112000786094038 7.41332851839187e-05 NA NA NA hsa-miR-28-5p CDKN1A 0.230152919654905 0.0742909586754846 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRanda;miRNATAP -0.556544696034112 2.23374029772338e-07 NA NA NA hsa-miR-30b-3p CDKN1A 0.834142820354026 0.000972582820878813 -1.29236263370998 3.21238784768704e-06 MirTarget -0.188277179739766 0.000464764881340986 NA NA NA hsa-miR-335-5p CDKN1A 1.77247705492746 2.22457658872091e-06 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate -0.15287461273454 1.73689594092226e-05 NA NA NA hsa-miR-345-5p CDKN1A 3.63532310411307 2.2698112107996e-21 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget -0.214757145120261 7.64097376843956e-11 NA NA NA hsa-miR-363-3p CDKN1A 1.50575300026701 0.00020976164678918 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase -0.119979037678532 0.000289602460917026 NA NA NA hsa-miR-423-3p CDKN1A 2.57855385753676 9.19765570228097e-24 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase -0.200481634838583 5.95960562762572e-05 NA NA NA hsa-miR-423-5p CDKN1A 0.963563145758616 9.86109015204915e-07 -1.29236263370998 3.21238784768704e-06 MirTarget -0.179252711950689 0.00923809529808823 NA NA NA hsa-miR-491-5p CDKN1A 0.615770352820862 0.0418295628761654 -1.29236263370998 3.21238784768704e-06 miRanda -0.127626893732512 0.00809072721464231 NA NA NA hsa-miR-503-5p CDKN1A 3.66513190181895 6.60300917645278e-21 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase -0.108479527294966 0.00188268400827089 NA NA NA hsa-miR-505-5p CDKN1A 1.89243395287645 9.82003586561819e-12 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;MirTarget -0.197864415222474 3.15355511624094e-05 NA NA NA hsa-miR-93-5p CDKN1A 3.03961860136032 1.11672209617395e-24 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.230725362813635 6.59990926283075e-08 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 2.35195531153712 5.45059325728841e-16 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate -0.260648356679274 4.62199208083779e-09 NA NA NA hsa-miR-96-5p CDKN1A 4.89433085814374 1.92000659886961e-31 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;miRTarBase -0.131322885353092 1.73810674264337e-05 26582573 Upregulation of microRNA 96 and its oncogenic functions by targeting CDKN1A in bladder cancer; Bioinformatics prediction combined with luciferase reporter assay were used to verify whether the cyclin-dependent kinase inhibitor CDKN1A was a potential target gene of miR-96; According to the data of miRTarBase CDKN1A might be a candidate target gene of miR-96; In addition luciferase reporter and Western blot assays respectively demonstrated that miR-96 could bind to the putative seed region in CDKN1A mRNA 3'UTR and significantly reduce the expression level of CDKN1A protein; Moreover we found that the inhibition of miR-96 expression remarkably decreased cell proliferation and promoted cell apoptosis of BC cell lines which was consistent with the findings observed following the introduction of CDKN1A cDNA without 3'UTR restored miR-96; Upregulation of miR-96 may contribute to aggressive malignancy partly through suppressing CDKN1A protein expression in BC cells bladder cancer hsa-miR-98-5p CDKN1A 1.11154309866014 1.24143909012085e-08 -1.29236263370998 3.21238784768704e-06 miRNAWalker2_validate;MirTarget -0.251133283878821 0.000264648620156197 NA NA NA hsa-miR-139-5p CHEK1 -1.52606598644743 1.20474151350665e-08 1.60009495890486 3.70484455888763e-14 miRanda -0.117325843833051 0.0025015103290901 NA NA NA hsa-let-7a-5p FAS 0.618599506841644 2.76973453058748e-05 -0.893121037137088 0.00675655818216232 TargetScan -0.294996828851615 0.0073114418152214 NA NA NA hsa-miR-106a-5p FAS 2.48882002962836 1.99757727374521e-12 -0.893121037137088 0.00675655818216232 miRNAWalker2_validate;miRTarBase -0.286433262215431 2.93487884151059e-11 22431000;27142596 miR 106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS; Bioinformatic analysis combining with validation experiments identified FAS as a direct target of miR-106a; Moreover a significant inverse correlation was found between miR-106a and FAS expression not only in gastric cancer cell lines but also in gastric cancer specimens; Taken together these findings suggest that ectopicly overexpressed miR-106a may play an oncogenic role in gastric carcinogenesis and impair extrinsic apoptotic pathway through targeting FAS;Functional experiment ascertained that miR-106a interacted with FAS and mediated caspase3 pathway tumorigenesis; gastric cancer;gastric cancer hsa-miR-23b-3p FAS -0.251132426825047 0.150200321210792 -0.893121037137088 0.00675655818216232 miRNATAP -0.391116610184141 2.37246599324584e-05 24356489 Moreover over-expression of Fas rescued the pro-proliferation effect of miR-23 indicating Fas is a direct mediator of miR-23 functions; Furthermore contrast to miR-23a/b which was up regulated the Fas expression level was down-regulated and inversely correlated with miR-23 in split radiation induced lymphoma tissue samples; Finally our data also indicates that miR-23a could repress Fas much more potent than miR-23b and the additional region besides conserved seed pairing enables miR-23a's higher regulation lymphoma hsa-miR-361-5p FAS 0.971859117235239 5.30236555707088e-09 -0.893121037137088 0.00675655818216232 miRanda -0.765596610913641 1.34005865686507e-16 NA NA NA hsa-miR-491-5p FAS 0.615770352820862 0.0418295628761654 -0.893121037137088 0.00675655818216232 miRanda -0.202143347610942 0.000355416125267629 NA NA NA hsa-miR-590-5p FAS 3.18300989862077 2.06813568597893e-26 -0.893121037137088 0.00675655818216232 miRanda -0.26445516527792 2.22188286955534e-07 NA NA NA hsa-miR-98-5p FAS 1.11154309866014 1.24143909012085e-08 -0.893121037137088 0.00675655818216232 miRNAWalker2_validate -0.770550954607501 1.78193866759893e-23 NA NA NA hsa-miR-130b-3p GADD45A 3.5350292994509 1.73934183852569e-27 -0.835839078652441 0.000140836693353512 MirTarget;miRNATAP -0.17218937101763 1.35434055056965e-08 NA NA NA hsa-miR-148b-3p GADD45A 1.97815018171325 6.07715327281595e-26 -0.835839078652441 0.000140836693353512 MirTarget -0.24164313540326 6.68989940877406e-06 NA NA NA hsa-miR-301a-3p GADD45A 2.81024345929638 2.29784847962242e-17 -0.835839078652441 0.000140836693353512 MirTarget;miRNATAP -0.112998413130989 0.000548458732723422 NA NA NA hsa-miR-324-5p GADD45A 2.95683728155095 1.32960514360912e-20 -0.835839078652441 0.000140836693353512 miRanda -0.112457495567732 0.000439233642516274 NA NA NA hsa-miR-421 GADD45A 1.17791565395543 6.78526676976629e-06 -0.835839078652441 0.000140836693353512 miRanda -0.133567846694638 0.00100215803079645 NA NA NA hsa-miR-454-3p GADD45A 2.47234127820054 1.12878018736016e-21 -0.835839078652441 0.000140836693353512 MirTarget;miRNATAP -0.123232081874385 0.00233561552908576 NA NA NA hsa-miR-590-3p GADD45A 2.59289455139753 1.03200520972022e-21 -0.835839078652441 0.000140836693353512 miRanda;mirMAP -0.116878603221423 0.00246827969058347 NA NA NA hsa-miR-1976 GADD45B 2.06550563678192 7.81396978619772e-18 -2.10522351126521 1.24009438337654e-11 miRNATAP -0.438571553390836 3.56919131064351e-13 NA NA NA hsa-miR-324-3p GADD45B 2.08739139768981 3.11885987067952e-13 -2.10522351126521 1.24009438337654e-11 MirTarget;miRNATAP -0.529388083839586 5.82182378729412e-27 NA NA NA hsa-miR-590-3p GADD45B 2.59289455139753 1.03200520972022e-21 -2.10522351126521 1.24009438337654e-11 miRanda -0.441564740260131 2.37587329223105e-16 NA NA NA hsa-miR-1976 GADD45G 2.06550563678192 7.81396978619772e-18 -1.14052103223901 0.000287911213048388 MirTarget -0.223301232794066 0.000270386119057603 NA NA NA hsa-miR-320a GADD45G 0.438165828017084 0.0390159073347133 -1.14052103223901 0.000287911213048388 miRanda -0.202148212458203 0.00541824995825031 NA NA NA hsa-miR-320b GADD45G 1.55692969039548 1.41071034251915e-08 -1.14052103223901 0.000287911213048388 miRanda -0.18771629419395 0.000624415279589848 NA NA NA hsa-miR-429 GADD45G 4.48815175680773 2.44117492218206e-17 -1.14052103223901 0.000287911213048388 miRNATAP -0.111263953867699 5.40997990169216e-05 NA NA NA hsa-miR-139-5p GTSE1 -1.52606598644743 1.20474151350665e-08 3.5224125178824 1.35755374370817e-34 miRanda -0.221504513309317 6.40372094528109e-05 NA NA NA hsa-let-7a-3p IGF1 1.42015538786275 7.03375209382654e-13 -3.75951127345565 1.26264890381366e-10 mirMAP -0.92426917804667 5.0725450948492e-11 NA NA NA hsa-let-7a-5p IGF1 0.618599506841644 2.76973453058748e-05 -3.75951127345565 1.26264890381366e-10 TargetScan -1.35484578107079 3.03432417502265e-12 NA NA NA hsa-let-7b-3p IGF1 0.868207752019623 1.30460056774366e-05 -3.75951127345565 1.26264890381366e-10 mirMAP -0.806527950481254 1.86996353532323e-08 NA NA NA hsa-let-7f-1-3p IGF1 1.55054488387679 7.31326775537952e-11 -3.75951127345565 1.26264890381366e-10 mirMAP -0.53583367534408 1.14955943868674e-05 NA NA NA hsa-miR-103a-2-5p IGF1 2.47599305985086 7.933028987326e-11 -3.75951127345565 1.26264890381366e-10 mirMAP -0.672288935268143 1.21134250789966e-16 NA NA NA hsa-miR-129-5p IGF1 -0.524170159980738 0.222582956006346 -3.75951127345565 1.26264890381366e-10 MirTarget;PITA;miRanda -0.24872412851026 0.000279350666200339 NA NA NA hsa-miR-130a-3p IGF1 2.02021855058359 1.26427102520415e-11 -3.75951127345565 1.26264890381366e-10 MirTarget -0.315325422858442 0.000861190344159203 NA NA NA hsa-miR-130b-3p IGF1 3.5350292994509 1.73934183852569e-27 -3.75951127345565 1.26264890381366e-10 MirTarget -0.781941096991651 4.63853079450254e-23 NA NA NA hsa-miR-15b-3p IGF1 2.33847028227712 4.29662877950836e-19 -3.75951127345565 1.26264890381366e-10 mirMAP -1.14217010288885 5.28861971423639e-31 NA NA NA hsa-miR-16-1-3p IGF1 2.56983217111073 3.02262181237943e-20 -3.75951127345565 1.26264890381366e-10 mirMAP -0.921958602704484 2.5787665170735e-21 NA NA NA hsa-miR-181a-5p IGF1 2.29556023480783 1.8138457900616e-21 -3.75951127345565 1.26264890381366e-10 mirMAP -0.642043297035051 1.32592036628183e-08 NA NA NA hsa-miR-181b-5p IGF1 2.49307679431293 3.92829250217627e-20 -3.75951127345565 1.26264890381366e-10 mirMAP -0.697159274066391 2.64064192584975e-12 NA NA NA hsa-miR-181c-5p IGF1 1.58587920618301 1.57506918816933e-10 -3.75951127345565 1.26264890381366e-10 mirMAP -0.418006737238249 0.00025755851863695 NA NA NA hsa-miR-186-5p IGF1 1.47445398888668 3.0781726595413e-15 -3.75951127345565 1.26264890381366e-10 mirMAP -1.20504446214237 1.61828209547411e-16 NA NA NA hsa-miR-19a-3p IGF1 3.42309334186656 3.02671914353412e-28 -3.75951127345565 1.26264890381366e-10 MirTarget -0.788529625251502 2.31580352219079e-21 NA NA NA hsa-miR-19b-1-5p IGF1 2.57951428759379 4.31576118703427e-20 -3.75951127345565 1.26264890381366e-10 mirMAP -0.995592930242643 1.89907484758614e-25 NA NA NA hsa-miR-19b-3p IGF1 2.49529833021187 1.57028265846699e-23 -3.75951127345565 1.26264890381366e-10 MirTarget -1.0369004238185 3.24569264936101e-23 NA NA NA hsa-miR-20a-3p IGF1 2.92621109947706 2.49504904635375e-17 -3.75951127345565 1.26264890381366e-10 mirMAP -0.809636638775284 3.3899709447565e-25 NA NA NA hsa-miR-224-3p IGF1 1.87536386559317 6.15140969394846e-05 -3.75951127345565 1.26264890381366e-10 mirMAP -0.362328718988192 1.01049263740062e-08 NA NA NA hsa-miR-26b-5p IGF1 0.885393662798425 3.2116297961629e-06 -3.75951127345565 1.26264890381366e-10 mirMAP -0.940665920848216 3.14292070875382e-10 NA NA NA hsa-miR-27a-3p IGF1 1.66542180327138 4.93775737894805e-13 -3.75951127345565 1.26264890381366e-10 miRNAWalker2_validate;miRTarBase -0.651692554274928 7.73576562076687e-08 NA NA NA hsa-miR-29b-3p IGF1 1.65828197923813 1.02676780888141e-09 -3.75951127345565 1.26264890381366e-10 MirTarget -0.519110555190393 5.59140789276621e-07 25592039 Luciferase reporter assays were conducted to determine the association between miR-29b and the insulin-like growth factor 1 IGF1 3' untranslated region 3'UTR; IGF1 an activator of PI3K/Akt signaling was confirmed as a novel target of miR-29b colorectal cancer hsa-miR-301a-3p IGF1 2.81024345929638 2.29784847962242e-17 -3.75951127345565 1.26264890381366e-10 MirTarget -0.801898835472881 2.70419582744703e-21 NA NA NA hsa-miR-3065-5p IGF1 2.74629919868982 1.86846589298284e-08 -3.75951127345565 1.26264890381366e-10 mirMAP -0.340906479396581 1.44245852597366e-06 NA NA NA hsa-miR-32-3p IGF1 2.02436999565685 5.94091197945307e-09 -3.75951127345565 1.26264890381366e-10 mirMAP -0.670318737511073 1.52748719371215e-13 NA NA NA hsa-miR-320a IGF1 0.438165828017084 0.0390159073347133 -3.75951127345565 1.26264890381366e-10 miRNATAP -0.87110280497324 9.95766777508728e-11 NA NA NA hsa-miR-320b IGF1 1.55692969039548 1.41071034251915e-08 -3.75951127345565 1.26264890381366e-10 miRNATAP -0.993279990978514 6.22780218264885e-24 NA NA NA hsa-miR-33a-3p IGF1 1.72765312072219 1.51784443453521e-07 -3.75951127345565 1.26264890381366e-10 MirTarget -0.862622681654972 2.52525229105421e-21 NA NA NA hsa-miR-3607-3p IGF1 2.68532138541128 5.66716351955047e-14 -3.75951127345565 1.26264890381366e-10 mirMAP -0.646493669321152 1.00233762873621e-16 NA NA NA hsa-miR-361-5p IGF1 0.971859117235239 5.30236555707088e-09 -3.75951127345565 1.26264890381366e-10 PITA;mirMAP -1.48168691679158 5.53863901928066e-19 NA NA NA hsa-miR-374b-3p IGF1 0.930487774873495 2.74420094310516e-05 -3.75951127345565 1.26264890381366e-10 mirMAP -0.498455115159369 0.000129551554566347 NA NA NA hsa-miR-3913-5p IGF1 2.44353493655328 8.95279496632448e-15 -3.75951127345565 1.26264890381366e-10 mirMAP -0.584661382885468 7.3573353974355e-11 NA NA NA hsa-miR-421 IGF1 1.17791565395543 6.78526676976629e-06 -3.75951127345565 1.26264890381366e-10 PITA -0.696572691037999 1.11839961387445e-10 NA NA NA hsa-miR-452-5p IGF1 1.37180058737183 0.00171494843537772 -3.75951127345565 1.26264890381366e-10 MirTarget;mirMAP -0.239891308263067 0.000253591244736158 NA NA NA hsa-miR-454-3p IGF1 2.47234127820054 1.12878018736016e-21 -3.75951127345565 1.26264890381366e-10 MirTarget -0.897384141255581 2.08224455125487e-17 NA NA NA hsa-miR-486-5p IGF1 -0.194913133034028 0.607377798807705 -3.75951127345565 1.26264890381366e-10 PITA;miRNATAP -0.336416232300254 9.17459110593128e-06 NA NA NA hsa-miR-576-5p IGF1 2.20467023993664 3.4053475193793e-19 -3.75951127345565 1.26264890381366e-10 PITA;mirMAP;miRNATAP -1.00551072603292 1.14064166854676e-20 NA NA NA hsa-miR-590-3p IGF1 2.59289455139753 1.03200520972022e-21 -3.75951127345565 1.26264890381366e-10 MirTarget;miRanda;mirMAP;miRNATAP -0.845684857318828 5.27514597787573e-17 NA NA NA hsa-miR-592 IGF1 1.51765968871077 0.000636335248013204 -3.75951127345565 1.26264890381366e-10 mirMAP -0.450455314337535 2.70073065710591e-12 NA NA NA hsa-miR-629-5p IGF1 2.10182419359635 1.51788571125757e-15 -3.75951127345565 1.26264890381366e-10 mirMAP -0.693559501922869 2.74994850835533e-11 NA NA NA hsa-miR-664a-3p IGF1 0.634721925861133 0.00520070178640421 -3.75951127345565 1.26264890381366e-10 mirMAP -0.350873716989665 0.00610206128936229 NA NA NA hsa-miR-940 IGF1 3.21011374578759 2.7923171128495e-14 -3.75951127345565 1.26264890381366e-10 MirTarget;PITA;miRNATAP -0.715084793572266 1.27978685880086e-25 NA NA NA hsa-miR-340-5p IGFBP3 0.30282999308409 0.157744789519131 1.07616866066205 0.0190221889164414 MirTarget -0.363812620560802 0.000495010806414192 NA NA NA hsa-let-7i-5p MDM4 0.412877696970403 0.00622731051981756 -0.0417166726103293 0.796356265058254 MirTarget -0.310234734345432 2.01306684248206e-09 NA NA NA hsa-miR-152-3p MDM4 -0.0480232621705712 0.844347735441565 -0.0417166726103293 0.796356265058254 MirTarget -0.198828633232754 2.62918534012835e-10 NA NA NA hsa-miR-21-3p MDM4 2.19296355304364 4.63665722759779e-15 -0.0417166726103293 0.796356265058254 MirTarget -0.181325523485394 5.97587010184892e-12 NA NA NA hsa-miR-21-5p MDM4 2.73773305747971 1.38864982139576e-43 -0.0417166726103293 0.796356265058254 miRNAWalker2_validate;mirMAP -0.177477843606092 5.08057823919745e-07 NA NA NA hsa-miR-22-5p MDM4 1.88011544159252 1.63978222572222e-16 -0.0417166726103293 0.796356265058254 MirTarget -0.199217576141233 9.07348970289514e-10 NA NA NA hsa-miR-222-5p MDM4 1.25632771414778 0.000138653440511922 -0.0417166726103293 0.796356265058254 mirMAP -0.135708562618783 4.32610541413678e-09 NA NA NA hsa-miR-27a-3p MDM4 1.66542180327138 4.93775737894805e-13 -0.0417166726103293 0.796356265058254 miRNATAP -0.13635021218626 3.33316430813592e-05 NA NA NA hsa-miR-29a-5p MDM4 1.46749674755271 3.34605004270294e-07 -0.0417166726103293 0.796356265058254 mirMAP -0.124414670397606 9.80377796420373e-06 NA NA NA hsa-miR-340-5p MDM4 0.30282999308409 0.157744789519131 -0.0417166726103293 0.796356265058254 mirMAP -0.100731792116629 0.00600243663457417 NA NA NA hsa-miR-424-5p MDM4 1.61930224569578 1.89642549274994e-10 -0.0417166726103293 0.796356265058254 mirMAP -0.10877766912749 0.000288454619788618 NA NA NA hsa-miR-766-3p MDM4 1.2456012547833 6.44584025471128e-06 -0.0417166726103293 0.796356265058254 MirTarget -0.170466790172467 5.73614835694442e-10 NA NA NA hsa-miR-92b-3p MDM4 1.20409245530001 3.41055816361655e-06 -0.0417166726103293 0.796356265058254 mirMAP -0.131207721486885 9.4896097992838e-06 NA NA NA hsa-miR-218-5p PERP -0.573801507392184 0.0551983860126703 0.990544293725591 0.00189868262679113 MirTarget -0.370641489866632 2.06250622676309e-13 NA NA NA hsa-miR-125a-5p PPM1D 0.599529391575274 0.0102300147180307 -0.219425016956042 0.154255437850909 miRanda -0.118313398242185 0.000201229247258851 NA NA NA hsa-miR-26b-5p PPM1D 0.885393662798425 3.2116297961629e-06 -0.219425016956042 0.154255437850909 miRNAWalker2_validate -0.174592863247596 6.80133924876196e-06 NA NA NA hsa-miR-29a-3p PPM1D -0.35074463683376 0.161238718982428 -0.219425016956042 0.154255437850909 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.132720969882317 7.36881241870101e-06 NA NA NA hsa-miR-29a-5p PPM1D 1.46749674755271 3.34605004270294e-07 -0.219425016956042 0.154255437850909 MirTarget -0.118099955048057 1.07774001244474e-05 NA NA NA hsa-miR-29b-3p PPM1D 1.65828197923813 1.02676780888141e-09 -0.219425016956042 0.154255437850909 MirTarget;miRNATAP -0.184456717251638 2.05968430674892e-12 NA NA NA hsa-miR-29c-3p PPM1D -0.00968972577705429 0.97099586346872 -0.219425016956042 0.154255437850909 miRNAWalker2_validate;MirTarget;miRNATAP -0.117275566662038 2.55081537019905e-05 25888625 A suppressive role of ionizing radiation responsive miR 29c in the development of liver carcinoma via targeting WIP1; miR-29c expression is downregulated in human hepatocellular carcinoma cells which is inversely correlated with WIP1 expression; miR-29c attenuates luciferase activity of a reporter harboring the 3'UTR binding motif of WIP1 mRNA; The biological effects of miR-29c may be mediated by its target WIP1 which regulates p53 activity via dephosphorylation at Ser-15; Finally fluorescence in situ hybridization FISH and immunohistochemical analyses indicate that miR-29c is downregulated in 50.6% of liver carcinoma tissues examined whereas WIP1 is upregulated in 45.4% of these tissues; The expression of miR-29c inversely correlates with that of WIP1 in HCC; Our results suggest that the IR-responsive miR-29c may function as a tumor suppressor that plays a crucial role in the development of liver carcinoma via targeting WIP1 therefore possibly representing a target molecule for therapeutic intervention for this disease liver cancer hsa-miR-320a PPM1D 0.438165828017084 0.0390159073347133 -0.219425016956042 0.154255437850909 miRanda -0.10506792390686 0.00286355431140724 NA NA NA hsa-miR-103a-3p PTEN 1.44083190496858 1.53799939616416e-13 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase -0.181124200363514 1.1176220548008e-07 26511107;24828205 LncRNA GAS5 induces PTEN expression through inhibiting miR 103 in endometrial cancer cells; To investigate the expression of GAS5 PTEN and miR-103 RT-PCR was performed; Finally we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells; Through inhibiting the expression of miR-103 GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis and thus could be an important mediator in the pathogenesis of endometrial cancer;Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN ; endometrial cancer;colorectal cancer hsa-miR-106a-5p PTEN 2.48882002962836 1.99757727374521e-12 -0.452835975443078 0.00142504206837966 miRNATAP -0.119115957971973 1.32932348443908e-10 26097565;26318586 miR 106a promotes growth and metastasis of non small cell lung cancer by targeting PTEN; Furthermore the presence of miR-106a was inversely correlated with PTEN in NSCLC tissues; Overall this study suggested that miR-106a inhibited the growth and metastasis of NSCLC cells by decreasing PTEN expression;Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo metastasis; lung squamous cell cancer;prostate cancer hsa-miR-106b-5p PTEN 2.46782146831112 1.36171495805632e-23 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase;miRNATAP -0.167925855213166 9.91778363511573e-11 24842611;26238857;26722252 MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance;Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells cell migration;drug resistance; gastric cancer;colorectal cancer;breast cancer hsa-miR-107 PTEN 1.30977326593435 1.30372468603965e-11 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;PITA;miRanda -0.125872445510677 0.000311847826997376 NA NA NA hsa-miR-130a-3p PTEN 2.02021855058359 1.26427102520415e-11 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.107066992505531 1.60679955529776e-06 26837847;24490491;27062783;22614869;27040383;27035216;26043084 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;Down-regulated miR-130a did not affect cell proliferations but enhanced the sensitivity of the cells to cisplatin inhibited the expressions of MDR1 mRNA and P-gp and increased the expression of PTEN proteins; MiR-130a inhibitor can reverse the cisplatin resistance by upregulating the expression of PTEN proteins and down-regulating P-gp in A2780 cell lines;Platinum-resistant patients had significantly higher levels of expression of miR-130a and BCL-2 and lower level of PTEN than platinum-sensitive patients P < 0.05; MiR-130a may mediate the generation of platinum resistance in epithelial ovarian cancer through inhibiting PTEN to activate PI3K/AKT signaling pathway and increasing BCL-2 to inhibit tumor cell apoptosis;We found that miR-130a was upregulated in SKOV3/CIS compared to the parental SKOV3 cells and PTEN was predicted to be the potential target of miR-130a;In addition by targeting PTEN 3' untranslated region miR-130a might increase cell growth and initiate protein kinase B AKT pathway activation;MicroRNA 130a promotes the metastasis and epithelial mesenchymal transition of osteosarcoma by targeting PTEN; MiR-130a exerted promoting effects on metastatic behavior and EMT of osteosarcoma cells through suppressing PTEN expression;This role of miR-130a may be achieved by regulating the MDR1 and PTEN gene expression cell migration;drug resistance;drug resistance;;;metastasis; bladder cancer;ovarian cancer;ovarian cancer;ovarian cancer;cervical and endocervical cancer;sarcoma;ovarian cancer hsa-miR-130b-3p PTEN 3.5350292994509 1.73934183852569e-27 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.118523549020844 1.10168456648735e-09 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-148a-3p PTEN 1.26728238710376 2.52325607402829e-06 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.102658849708203 4.6220389940495e-05 22496917 Introduction of anti-miR-148a increased PTEN protein and mRNA expression suggesting that PTEN was targeted by miR-148a liver cancer hsa-miR-148b-3p PTEN 1.97815018171325 6.07715327281595e-26 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.168985576302433 9.27873273241405e-07 NA NA NA hsa-miR-15b-3p PTEN 2.33847028227712 4.29662877950836e-19 -0.452835975443078 0.00142504206837966 mirMAP -0.110608587315208 9.86982828042873e-06 NA NA NA hsa-miR-16-2-3p PTEN 2.31623086727196 4.29838149388188e-20 -0.452835975443078 0.00142504206837966 mirMAP -0.10224879739163 8.32790317307332e-05 NA NA NA hsa-miR-17-5p PTEN 3.2710598467455 9.93128340539455e-27 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.128957385840206 5.08699518968894e-10 27400681;23391506;23133552;26629823;24462867;26318586;26215320;25634356;26500892;24912422;23418359 GFRα2 prompts cell growth and chemoresistance through down regulating tumor suppressor gene PTEN via Mir 17 5p in pancreatic cancer; Mechanically we discovered that high GFRα2 expression level leads to PTEN inactivation via enhancing Mir-17-5p level;We found that these phenotypes were the results of miR-17 targeting PTEN;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;We hypothesized that knocking down the oncogenic microRNA oncomiR miR-17-5p might restore the expression levels of PDCD4 and PTEN tumor suppressor proteins illustrating a route to oligonucleotide therapy of TNBC; Contrary to conventional wisdom antisense knockdown of oncomiR miR-17-5p guide strand reduced PDCD4 and PTEN proteins by 1.8±0.3 fold in human TNBC cells instead of raising them; Bioinformatics analysis and folding energy calculations revealed that mRNA targets of miR-17-5p guide strand such as PDCD4 and PTEN could also be regulated by miR-17-3p passenger strand;miR 17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression; Expression of miR-17 was negatively correlated with PTEN in OS tissues;Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR 17 family in prostate cancer; Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo;In addition ERβ expression significantly increased in calycosin-treated HCT-116 cells followed by a decrease of miR-17 and up-regulation of PTEN; Our results indicate that calycosin has an inhibitory effect on CRC which might be obtained by ERβ-mediated regulation of miR-17 and PTEN expression;The High Expression of the microRNA 17 92 Cluster and its Paralogs and the Downregulation of the Target Gene PTEN Is Associated with Primary Cutaneous B Cell Lymphoma Progression;MicroRNA 17 5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling; miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells;MicroRNA 17 5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression; We found that PTEN was a target of miR-17-5p in the colon cancer cells and their context-specific interactions were responsible for multiple drug-resistance; Chemotherapy was found to increase the expression levels of miR-17-5p which further repressed PTEN levels contributing to the development of chemo-resistance; MiR-17-5p is a predictive factor for chemotherapy response and a prognostic factor for overall survival in CRC which is due to its regulation of PTEN expression;The mature miR-17-5p exerted this function by repressing the expression of PTEN drug resistance;;;;metastasis;;;progression;drug resistance;metastasis;drug resistance;poor survival; pancreatic cancer;glioblastoma;sarcoma;breast cancer;sarcoma;prostate cancer;colorectal cancer;B cell lymphoma;ovarian cancer;colorectal cancer;liver cancer hsa-miR-181c-5p PTEN 1.58587920618301 1.57506918816933e-10 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.127904121862523 2.20738602419631e-06 25695913 miR 181c promotes proliferation via suppressing PTEN expression in inflammatory breast cancer; In this study we showed that miR-181c as an oncogene promoted proliferation and it inhibited PTEN protein expression by targeting 3'-UTR of PTEN mRNA in IBC SUM149 cells; Thus targeting miR-181c and restoration of PTEN can be used in conjunction with other therapies to prevent progression of IBC progression breast cancer hsa-miR-181d-5p PTEN 1.52204912185579 9.2693319057748e-08 -0.452835975443078 0.00142504206837966 MirTarget -0.117066047918988 7.16131240625052e-07 27006767 Moreover hsa-miR-181d was upregulated to control expression a tumor suppressor gene PTEN to protect the cancer cell from apoptosis endometrial cancer hsa-miR-186-5p PTEN 1.47445398888668 3.0781726595413e-15 -0.452835975443078 0.00142504206837966 mirMAP;miRNATAP -0.221807059815679 3.24247402491438e-10 NA NA NA hsa-miR-188-5p PTEN 2.52825126596644 6.78099435999778e-14 -0.452835975443078 0.00142504206837966 MirTarget;PITA;miRNATAP -0.109812572617288 1.33705979957174e-07 NA NA NA hsa-miR-18a-5p PTEN 3.78535678765353 3.72446596213886e-26 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase -0.11423116163821 1.11808022110275e-09 24681249;27291152 However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis;miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis metastasis; colon cancer;esophageal cancer hsa-miR-193a-3p PTEN 1.60460546928078 2.86169793322984e-07 -0.452835975443078 0.00142504206837966 PITA;miRanda -0.105979704931185 8.32668652705572e-07 26753960;23223432 Downregulation of microRNA 193 3p inhibits tumor proliferation migration and chemoresistance in human gastric cancer by regulating PTEN gene;Our study identifies miR-193a and PTEN as targets for AML1/ETO and provides evidence that links the epigenetic silencing of tumor suppressor genes miR-193a and PTEN to differentiation block of myeloid precursors drug resistance;differentiation gastric cancer;acute myeloid leukemia hsa-miR-19a-3p PTEN 3.42309334186656 3.02671914353412e-28 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.128463306420323 2.55472255726088e-10 25107371;27445062;26098000;24831732;21853360;24681249;27289489 The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid; Meanwhile gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN; The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN;The target genes of miR-19a such as ABCA1 and PTEN that had been suppressed by miR recovered their expression through CAP treatment;Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN;Transfection of miR-19a and -19b mimics reversed the up-regulations of IGF2R and PTEN gene expression and abrogated the GSE induced anti-proliferative response ;;;;drug resistance;metastasis;drug resistance bladder cancer;breast cancer;lung cancer;breast cancer;breast cancer;colon cancer;lung cancer hsa-miR-19b-3p PTEN 2.49529833021187 1.57028265846699e-23 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.17043189646512 2.79115195694575e-11 26098000;24831732;21853360;24681249 Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN ;;drug resistance;metastasis lung cancer;breast cancer;breast cancer;colon cancer hsa-miR-20a-5p PTEN 3.15577933619191 4.19753346442454e-25 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase;miRNATAP -0.12193642920165 5.77479259337628e-09 26031366 The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction; MiR-20a levels were increased in HCC cell lines and tissues whereas PTEN was inversely correlated with it; PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance drug resistance liver cancer hsa-miR-25-3p PTEN 1.36472605225697 4.83790725935681e-11 -0.452835975443078 0.00142504206837966 miRTarBase;MirTarget;miRNATAP -0.196462680546189 9.04767751306264e-10 NA NA NA hsa-miR-301a-3p PTEN 2.81024345929638 2.29784847962242e-17 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.123344981900917 2.76158051949627e-09 24315818;26846737 Upregulated microRNA 301a in breast cancer promotes tumor metastasis by targeting PTEN and activating Wnt/β catenin signaling; Furthermore miR-301a directly targeted and suppressed PTEN one negative regulator of the Wnt/β-catenin signaling cascade; These results demonstrate that miR-301a maintains constitutively activated Wnt/β-catenin signaling by directly targeting PTEN which promotes breast cancer invasion and metastasis;MicroRNA 301a promotes cell proliferation via PTEN targeting in Ewing's sarcoma cells; Our results demonstrated the novel mechanism controlling PTEN expression via miR-301a in ES cells metastasis; breast cancer;sarcoma hsa-miR-30b-5p PTEN 0.800822374912712 0.000133601914525413 -0.452835975443078 0.00142504206837966 mirMAP -0.15403930655305 2.2546089672267e-06 NA NA NA hsa-miR-30c-5p PTEN 0.778774633108 0.000285599212429136 -0.452835975443078 0.00142504206837966 mirMAP -0.150378554405459 2.29281374408959e-06 NA NA NA hsa-miR-30d-3p PTEN 0.977747550180601 4.3048212205381e-05 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.119783791946268 2.73757307106354e-05 NA NA NA hsa-miR-30d-5p PTEN 0.675423997639582 0.00270618816039773 -0.452835975443078 0.00142504206837966 mirMAP -0.11072634618103 0.000287709940594657 NA NA NA hsa-miR-30e-5p PTEN 1.23904909539663 1.35561917122157e-09 -0.452835975443078 0.00142504206837966 mirMAP -0.160981204851498 1.03953643650469e-06 NA NA NA hsa-miR-32-5p PTEN 2.34056473219422 6.24134585974322e-20 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.145568223802877 9.281864133748e-09 24123284;25647261;23617834 In this study we determined the levels of the correlation between and the clinical significance of the expression of miR-32 and phosphatase and tensin homologue PTEN a tumor suppressor targeted by miR-32 in CRC; The levels of miR-32 and PTEN gene expression in 35 colorectal carcinoma samples 35 corresponding cancer-adjacent tissue samples 27 colorectal adenoma samples and 16 normal tissue samples were quantified using real-time quantitative reverse transcriptase-polymerase chain reaction; The relationship between the miR-32 and PTEN protein expression and clinicopathological factors was analyzed; Significant upregulation of miR-32 expression and reduction of PTEN were identified in CRC tissues; An inverse relationship between miR-32 and PTEN protein expression was identified; MiR-32 and PTEN expression were inversely correlated and miR-32 may be associated with the development of CRC;MiR 32 induces cell proliferation migration and invasion in hepatocellular carcinoma by targeting PTEN; Besides miRNA-32 down-regulates PTEN through binding to 3'-UTR of PTEN mRNA from luciferase reporter assay and the expression level of miR-32 could affect the proliferation migration and invasion of liver cancer cell lines via PTEN/Akt signaling pathway; Down-expression of PTEN could significantly attenuate the inhibitory effects of knockdown miR-32 on the proliferation migration and invasion of liver cancer cells suggesting that miR-32 could be a potential target for HCC treatment;MicroRNA 32 miR 32 regulates phosphatase and tensin homologue PTEN expression and promotes growth migration and invasion in colorectal carcinoma cells; In this study we identified the potential effects of miR-32 on some important biological properties of CRC cells and clarified the regulation of PTEN by miR-32; The 3'-untranslated region 3'-UTR of PTEN combined with miR-32 was verified by dual-luciferase reporter assay; Gain-of-function and loss-of-function studies showed that overexpression of miR-32 promoted SW480 cell proliferation migration and invasion reduced apoptosis and resulted in downregulation of PTEN at a posttranscriptional level; However miR-32 knock-down inhibited these processes in HCT-116 cells and enhanced the expression of PTEN protein; In addition we further identified PTEN as the functional downstream target of miR-32 by directly targeting the 3'-UTR of PTEN; Our results demonstrated that miR-32 was involved in tumorigenesis of CRC at least in part by suppression of PTEN ;;tumorigenesis colorectal cancer;liver cancer;colorectal cancer hsa-miR-362-3p PTEN 2.0756816987665 3.89026064579331e-12 -0.452835975443078 0.00142504206837966 miRanda -0.115158238382692 5.24016404806603e-07 NA NA NA hsa-miR-363-3p PTEN 1.50575300026701 0.00020976164678918 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.100220872472703 1.35166197069511e-09 NA NA NA hsa-miR-374a-5p PTEN 0.484615721953736 0.0042981729815149 -0.452835975443078 0.00142504206837966 MirTarget;mirMAP -0.154385867426133 0.000159063773613779 NA NA NA hsa-miR-374b-5p PTEN 0.0654362503857184 0.722673188879465 -0.452835975443078 0.00142504206837966 MirTarget;mirMAP;miRNATAP -0.119395270830768 0.00157844251632807 NA NA NA hsa-miR-421 PTEN 1.17791565395543 6.78526676976629e-06 -0.452835975443078 0.00142504206837966 miRanda -0.116149976062421 7.77520989006685e-06 NA NA NA hsa-miR-425-5p PTEN 3.07071373939774 4.02947460586085e-25 -0.452835975443078 0.00142504206837966 miRNATAP -0.139167264143658 8.36425767948797e-11 25154996 An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation gastric cancer hsa-miR-454-3p PTEN 2.47234127820054 1.12878018736016e-21 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.143498211514651 2.3895399060586e-08 26296312;27261580 MicroRNA 454 functions as an oncogene by regulating PTEN in uveal melanoma; Furthermore we identified PTEN as a direct target of miR-454; Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells;MiR 454 promotes the progression of human non small cell lung cancer and directly targets PTEN; At last the potential regulatory function of miR-454 on PTEN expression was confirmed; Further PTEN was confirmed as a direct target of miR-454 by using Luciferase Reporter Assay ;progression melanoma;lung squamous cell cancer hsa-miR-484 PTEN 1.81844463411787 1.82951571230938e-15 -0.452835975443078 0.00142504206837966 miRNATAP -0.123242919063894 2.18866967044909e-05 NA NA NA hsa-miR-532-5p PTEN 1.55697650978597 1.57884735535196e-11 -0.452835975443078 0.00142504206837966 PITA;mirMAP;miRNATAP -0.144490491618683 5.83499130267459e-07 NA NA NA hsa-miR-590-5p PTEN 3.18300989862077 2.06813568597893e-26 -0.452835975443078 0.00142504206837966 mirMAP -0.121253713178443 3.04555241061884e-08 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-7-1-3p PTEN 1.84950311431067 1.25300363397654e-13 -0.452835975443078 0.00142504206837966 mirMAP -0.133579523943701 4.98334583211167e-07 NA NA NA hsa-miR-92a-3p PTEN 2.06460102658973 1.44784291084757e-19 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.175671660591697 6.62046894313789e-10 26432332;25515201;24137349;23546593;23133552;24026406 Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor; Taken together these findings suggested that miR-92a could promote growth metastasis and chemoresistance in NSCLC cells at least partially by targeting PTEN;MiR 92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN Mediated PI3K/AKT Pathway; The expression of miR-92a PTEN and E-cadherin was analyzed by real-time PCR; In addition there was a negative correlation between levels of miR-92a and the PTEN gene p < 0.0001; The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;The expression levels of miR-92a and phosphatase and tensin homologue PTEN were detected by qRT-PCR and western blot; In addition the regulation of PTEN by miR-92a was evaluated by qRT-PCR western blot and luciferase reporter assays; There was an inverse correlation between the levels of miR-92a and PTEN in CRC tissues; The overexpression of miR-92a in CRC cells decreased PTEN expression at the translational level and decreased PTEN-driven luciferase-reporter activity; Our results demonstrated that miR-92a induced EMT and regulated cell growth migration and invasion in the SW480 cells at least partially via suppression of PTEN expression metastasis;drug resistance;metastasis;cell migration;;worse prognosis;; lung squamous cell cancer;colorectal cancer;cervical and endocervical cancer;liver cancer;sarcoma;colorectal cancer hsa-miR-92b-3p PTEN 1.20409245530001 3.41055816361655e-06 -0.452835975443078 0.00142504206837966 MirTarget;miRNATAP -0.114974293673639 1.10439131275336e-05 24099768;26878388;24137349;23546593 MiR 92b regulates the cell growth cisplatin chemosensitivity of A549 non small cell lung cancer cell line and target PTEN; Furthermore we found miR-92b could directly target PTEN a unique tumor suppressor gene which was downregulated in lung cancer tissues compared to the matched adjacent normal tissues;We revealed that patients exhibiting an upregulation of hsa-miR-92b and patients with deletions of PTEN did not tend to overlap and hsa-miR-92b and PTEN coordinately regulated the pathway of 'cell cycle' and so on;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC ;;;worse prognosis lung squamous cell cancer;breast cancer;cervical and endocervical cancer;liver cancer hsa-miR-93-5p PTEN 3.03961860136032 1.11672209617395e-24 -0.452835975443078 0.00142504206837966 miRNAWalker2_validate;miRTarBase;miRNATAP -0.134735169835666 3.72873311860152e-10 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-338-3p RPRM 0.453166438193782 0.144583389707558 -2.44674944201373 5.13177314422131e-05 miRanda -0.366653541373726 0.000108153242770562 NA NA NA hsa-miR-100-5p RRM2 -1.48442967087177 1.5610956769298e-05 3.09406037621288 4.77730173839214e-23 miRNAWalker2_validate -0.124706499850974 0.00689484361217717 NA NA NA hsa-miR-125a-5p RRM2 0.599529391575274 0.0102300147180307 3.09406037621288 4.77730173839214e-23 miRanda -0.232721746714457 0.000699122664729244 NA NA NA hsa-miR-30a-5p RRM2 -0.878357906436456 0.000509952992366057 3.09406037621288 4.77730173839214e-23 miRNAWalker2_validate -0.190291426998104 0.00260478219816202 NA NA NA hsa-miR-129-5p SERPINE1 -0.524170159980738 0.222582956006346 -0.393407117230654 0.370425399232706 miRanda -0.169701923663541 0.000735839704611328 NA NA NA hsa-miR-148a-5p SERPINE1 1.16173838132242 0.000148929299881612 -0.393407117230654 0.370425399232706 miRNATAP -0.285071111266077 3.2328150503793e-05 NA NA NA hsa-miR-2110 SERPINE1 1.16625590651194 6.4784195289885e-05 -0.393407117230654 0.370425399232706 miRNATAP -0.312861484463966 5.27602364952907e-05 NA NA NA hsa-miR-301a-3p SERPINE1 2.81024345929638 2.29784847962242e-17 -0.393407117230654 0.370425399232706 miRNAWalker2_validate;miRTarBase -0.298339721528262 3.88735440627883e-06 NA NA NA hsa-miR-30b-5p SERPINE1 0.800822374912712 0.000133601914525413 -0.393407117230654 0.370425399232706 MirTarget -0.589742716683692 3.53023994480113e-09 25170877 miR-30b may function as a novel tumor suppressor gene in gastric cancer by targeting PAI-1 and regulating the apoptosis of cancer cells gastric cancer hsa-miR-30c-5p SERPINE1 0.778774633108 0.000285599212429136 -0.393407117230654 0.370425399232706 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.50652750448866 2.38346715661289e-07 NA NA NA hsa-miR-30d-5p SERPINE1 0.675423997639582 0.00270618816039773 -0.393407117230654 0.370425399232706 miRNATAP -0.643924984116999 3.16202278334948e-12 NA NA NA hsa-miR-378a-5p SERPINE1 0.223456081148306 0.386368843153575 -0.393407117230654 0.370425399232706 MirTarget -0.404559892121807 7.98544569711981e-07 NA NA NA hsa-miR-421 SERPINE1 1.17791565395543 6.78526676976629e-06 -0.393407117230654 0.370425399232706 miRanda -0.395794324632837 7.58532570850472e-07 NA NA NA hsa-miR-425-5p SERPINE1 3.07071373939774 4.02947460586085e-25 -0.393407117230654 0.370425399232706 MirTarget;miRNATAP -0.268582077948886 6.45365175266955e-05 NA NA NA hsa-miR-590-3p SERPINE1 2.59289455139753 1.03200520972022e-21 -0.393407117230654 0.370425399232706 miRanda -0.275014479514724 0.000327361176519488 NA NA NA hsa-miR-769-5p SERPINE1 1.82023760596845 9.6815886564134e-14 -0.393407117230654 0.370425399232706 MirTarget;miRNATAP -0.571996036825274 4.60171798625337e-12 NA NA NA hsa-miR-942-5p SERPINE1 2.35195531153712 5.45059325728841e-16 -0.393407117230654 0.370425399232706 MirTarget -0.197923564863346 0.00510222008258954 NA NA NA hsa-miR-146b-5p SESN1 1.75587235896724 1.15724631266659e-07 -0.493278465606398 0.00887442896202912 miRanda -0.150713243841565 1.83819263352391e-08 NA NA NA hsa-miR-15b-5p SESN1 1.57112623124278 9.14247846750597e-13 -0.493278465606398 0.00887442896202912 MirTarget;miRNATAP -0.174517924661756 1.54630824586367e-05 NA NA NA hsa-miR-16-5p SESN1 1.75824877145857 1.68592440033108e-16 -0.493278465606398 0.00887442896202912 MirTarget;miRNATAP -0.123692410102397 0.00287565212861843 NA NA NA hsa-miR-18a-3p SESN1 2.14813400940323 2.23460809936028e-06 -0.493278465606398 0.00887442896202912 MirTarget -0.117993460415431 2.14882518148023e-06 NA NA NA hsa-miR-21-5p SESN1 2.73773305747971 1.38864982139576e-43 -0.493278465606398 0.00887442896202912 miRNAWalker2_validate -0.206141304526145 6.43975390937491e-07 NA NA NA hsa-miR-24-3p SESN1 0.99989036772428 1.35595013650376e-07 -0.493278465606398 0.00887442896202912 miRNAWalker2_validate;MirTarget;miRNATAP -0.150170953056088 0.00172400628763271 NA NA NA hsa-miR-362-3p SESN1 2.0756816987665 3.89026064579331e-12 -0.493278465606398 0.00887442896202912 miRanda -0.11955370793689 9.60015186979642e-05 NA NA NA hsa-miR-424-5p SESN1 1.61930224569578 1.89642549274994e-10 -0.493278465606398 0.00887442896202912 MirTarget;miRNATAP -0.136212671174791 0.000105145919169288 NA NA NA hsa-miR-590-3p SESN1 2.59289455139753 1.03200520972022e-21 -0.493278465606398 0.00887442896202912 miRanda -0.134127489805024 4.39538572907697e-05 NA NA NA hsa-miR-106b-5p SESN3 2.46782146831112 1.36171495805632e-23 -0.956665908760363 0.0236707121906867 MirTarget -0.33896555624124 1.55582690349284e-05 NA NA NA hsa-miR-130b-3p SESN3 3.5350292994509 1.73934183852569e-27 -0.956665908760363 0.0236707121906867 mirMAP -0.266537918703233 5.28864553417762e-06 NA NA NA hsa-miR-136-5p SESN3 2.09637138292451 1.50140005592201e-10 -0.956665908760363 0.0236707121906867 mirMAP -0.184181900600659 0.00263456919290343 NA NA NA hsa-miR-16-2-3p SESN3 2.31623086727196 4.29838149388188e-20 -0.956665908760363 0.0236707121906867 mirMAP -0.209090747164236 0.00723232316920077 NA NA NA hsa-miR-17-5p SESN3 3.2710598467455 9.93128340539455e-27 -0.956665908760363 0.0236707121906867 MirTarget;TargetScan -0.224566553303425 0.000348108216357546 NA NA NA hsa-miR-181a-5p SESN3 2.29556023480783 1.8138457900616e-21 -0.956665908760363 0.0236707121906867 MirTarget -0.246189862598435 0.00239715906784803 NA NA NA hsa-miR-181c-5p SESN3 1.58587920618301 1.57506918816933e-10 -0.956665908760363 0.0236707121906867 MirTarget -0.300853714278903 0.000202418700687206 NA NA NA hsa-miR-181d-5p SESN3 1.52204912185579 9.2693319057748e-08 -0.956665908760363 0.0236707121906867 MirTarget -0.211659538037241 0.00289476401343307 NA NA NA hsa-miR-186-5p SESN3 1.47445398888668 3.0781726595413e-15 -0.956665908760363 0.0236707121906867 MirTarget -0.440852718571142 3.52293070364062e-05 NA NA NA hsa-miR-200b-3p SESN3 3.78235093249346 3.18343003239465e-14 -0.956665908760363 0.0236707121906867 MirTarget;TargetScan -0.106241424665915 0.00755083660436466 NA NA NA hsa-miR-20a-3p SESN3 2.92621109947706 2.49504904635375e-17 -0.956665908760363 0.0236707121906867 mirMAP -0.162720897619173 0.00546768159082076 NA NA NA hsa-miR-20a-5p SESN3 3.15577933619191 4.19753346442454e-25 -0.956665908760363 0.0236707121906867 MirTarget -0.21904059070653 0.000532178570750604 NA NA NA hsa-miR-25-3p SESN3 1.36472605225697 4.83790725935681e-11 -0.956665908760363 0.0236707121906867 MirTarget;miRNATAP -0.295465247523023 0.00237188618531322 NA NA NA hsa-miR-29a-5p SESN3 1.46749674755271 3.34605004270294e-07 -0.956665908760363 0.0236707121906867 MirTarget;mirMAP -0.23270238610793 0.00177966440161202 NA NA NA hsa-miR-301a-3p SESN3 2.81024345929638 2.29784847962242e-17 -0.956665908760363 0.0236707121906867 mirMAP -0.215901582490512 0.000575463961354677 NA NA NA hsa-miR-32-3p SESN3 2.02436999565685 5.94091197945307e-09 -0.956665908760363 0.0236707121906867 mirMAP -0.231905173439685 0.000430877492884215 NA NA NA hsa-miR-32-5p SESN3 2.34056473219422 6.24134585974322e-20 -0.956665908760363 0.0236707121906867 MirTarget;miRNATAP -0.386513014230849 3.49776100391495e-07 NA NA NA hsa-miR-335-3p SESN3 3.08791651139852 5.31309869723517e-12 -0.956665908760363 0.0236707121906867 mirMAP -0.174469531164113 8.26718849255371e-05 NA NA NA hsa-miR-335-5p SESN3 1.77247705492746 2.22457658872091e-06 -0.956665908760363 0.0236707121906867 miRNAWalker2_validate -0.161616070620178 0.00277730966125122 NA NA NA hsa-miR-429 SESN3 4.48815175680773 2.44117492218206e-17 -0.956665908760363 0.0236707121906867 MirTarget -0.127752871629512 0.000551851138551874 NA NA NA hsa-miR-450b-5p SESN3 1.69244418548325 1.68064054148881e-10 -0.956665908760363 0.0236707121906867 MirTarget;PITA;mirMAP;miRNATAP -0.214193495471289 0.00478616696239884 NA NA NA hsa-miR-454-3p SESN3 2.47234127820054 1.12878018736016e-21 -0.956665908760363 0.0236707121906867 mirMAP -0.276264524566 0.000363209940334692 NA NA NA hsa-miR-511-5p SESN3 1.04499312543772 0.00234126154508174 -0.956665908760363 0.0236707121906867 MirTarget -0.16373363322762 0.00597505382792804 NA NA NA hsa-miR-576-5p SESN3 2.20467023993664 3.4053475193793e-19 -0.956665908760363 0.0236707121906867 mirMAP -0.335376383584556 2.45844944199642e-05 NA NA NA hsa-miR-589-3p SESN3 2.51971841868825 2.39125940264107e-11 -0.956665908760363 0.0236707121906867 MirTarget;mirMAP -0.174890942818206 0.003775017074676 NA NA NA hsa-miR-590-3p SESN3 2.59289455139753 1.03200520972022e-21 -0.956665908760363 0.0236707121906867 miRNAWalker2_validate;MirTarget;miRanda -0.261901277606811 0.000393768456575988 NA NA NA hsa-miR-93-5p SESN3 3.03961860136032 1.11672209617395e-24 -0.956665908760363 0.0236707121906867 MirTarget -0.246609278354896 0.000150167372344779 NA NA NA hsa-miR-96-5p SESN3 4.89433085814374 1.92000659886961e-31 -0.956665908760363 0.0236707121906867 MirTarget;TargetScan -0.148005009716274 0.00141667107991623 NA NA NA hsa-miR-195-5p STEAP3 -1.44753678070504 8.70594292149271e-09 1.30602544970675 1.23014964215525e-06 MirTarget -0.213131877499917 3.41247363720204e-05 NA NA NA hsa-let-7a-3p THBS1 1.42015538786275 7.03375209382654e-13 -2.1277814514878 2.63898598170147e-08 mirMAP -0.324256229204835 0.000499512291196202 NA NA NA hsa-let-7a-5p THBS1 0.618599506841644 2.76973453058748e-05 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate;TargetScan -0.793931296421232 4.01263206453322e-10 NA NA NA hsa-let-7b-5p THBS1 0.604314269683977 0.00140009936153214 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate -0.3783572455659 0.00015103278303974 NA NA NA hsa-let-7d-5p THBS1 1.33236798905207 1.1741085658466e-13 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate -0.859220357114439 4.03379037942602e-18 NA NA NA hsa-miR-151a-3p THBS1 1.24018761527407 1.08839919675408e-07 -2.1277814514878 2.63898598170147e-08 MirTarget -0.694569328638858 1.06745479684608e-19 NA NA NA hsa-miR-16-1-3p THBS1 2.56983217111073 3.02262181237943e-20 -2.1277814514878 2.63898598170147e-08 MirTarget -0.547933930739432 9.0287268587793e-18 NA NA NA hsa-miR-17-5p THBS1 3.2710598467455 9.93128340539455e-27 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate -0.535410262786805 2.01390645555519e-22 NA NA NA hsa-miR-181a-5p THBS1 2.29556023480783 1.8138457900616e-21 -2.1277814514878 2.63898598170147e-08 mirMAP -0.311799903081761 2.56428301535389e-05 NA NA NA hsa-miR-181b-5p THBS1 2.49307679431293 3.92829250217627e-20 -2.1277814514878 2.63898598170147e-08 mirMAP -0.286545329851077 1.38651496488125e-05 NA NA NA hsa-miR-181c-5p THBS1 1.58587920618301 1.57506918816933e-10 -2.1277814514878 2.63898598170147e-08 mirMAP -0.509013456937225 2.75401083927959e-12 NA NA NA hsa-miR-182-5p THBS1 3.53923166221814 5.07781258121574e-21 -2.1277814514878 2.63898598170147e-08 MirTarget -0.464276709680251 4.8914160255443e-25 24053448 Effects of anti miR 182 on TSP 1 expression in human colon cancer cells: there is a sense in antisense?; We investigate on the molecular mechanism by which miR-182 could regulate thrombospondin-1 TSP-1 expression a protein downregulated in CRC and inversely correlated with tumor vascularity and metastasis; miR-182 over-expressed in colorectal cancer CRC has like predictive target thrombospondin-1 TSP-1 a protein inversely correlated with tumor vascularity and metastasis that results downregulated in different types of cancer including CRC; We found that TSP-1 increased after transfection with anti-miR-182 and we showed that miR-182 targets TSP-1 3'UTR-mRNA in both cells; Our data suggest that miR-182 targets the anti-angiogenic factor TSP-1 and that anti-miR-182 determines an upregulation of TSP-1 expression in colon cancer cells metastasis colon cancer hsa-miR-186-5p THBS1 1.47445398888668 3.0781726595413e-15 -2.1277814514878 2.63898598170147e-08 mirMAP -0.790631830268256 8.0462867743067e-17 NA NA NA hsa-miR-18a-5p THBS1 3.78535678765353 3.72446596213886e-26 -2.1277814514878 2.63898598170147e-08 MirTarget -0.35804586565731 2.12561579001182e-12 NA NA NA hsa-miR-19a-3p THBS1 3.42309334186656 3.02671914353412e-28 -2.1277814514878 2.63898598170147e-08 MirTarget;mirMAP -0.479011511951586 1.31597383903182e-18 NA NA NA hsa-miR-19b-3p THBS1 2.49529833021187 1.57028265846699e-23 -2.1277814514878 2.63898598170147e-08 MirTarget;mirMAP -0.632469943962746 2.39270341905539e-20 NA NA NA hsa-miR-200a-5p THBS1 3.86316681673704 1.81076942093171e-15 -2.1277814514878 2.63898598170147e-08 MirTarget -0.396991686050601 3.54394977146249e-30 NA NA NA hsa-miR-200b-5p THBS1 2.54484902707822 1.59240467582976e-06 -2.1277814514878 2.63898598170147e-08 MirTarget -0.404286942819477 1.05447906162297e-27 NA NA NA hsa-miR-20a-5p THBS1 3.15577933619191 4.19753346442454e-25 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate -0.555674406219999 6.63212782868049e-24 NA NA NA hsa-miR-3065-5p THBS1 2.74629919868982 1.86846589298284e-08 -2.1277814514878 2.63898598170147e-08 MirTarget -0.272591193657042 2.35925406869001e-09 NA NA NA hsa-miR-32-5p THBS1 2.34056473219422 6.24134585974322e-20 -2.1277814514878 2.63898598170147e-08 mirMAP -0.532149898174492 6.38170990221277e-15 NA NA NA hsa-miR-335-3p THBS1 3.08791651139852 5.31309869723517e-12 -2.1277814514878 2.63898598170147e-08 mirMAP -0.184976537215221 5.11650202408596e-06 NA NA NA hsa-miR-33a-3p THBS1 1.72765312072219 1.51784443453521e-07 -2.1277814514878 2.63898598170147e-08 MirTarget;mirMAP -0.543323514403514 5.49591905485477e-20 NA NA NA hsa-miR-375 THBS1 1.13910305294079 0.0512283522214186 -2.1277814514878 2.63898598170147e-08 miRanda -0.150506420398144 2.2392975982835e-06 NA NA NA hsa-miR-421 THBS1 1.17791565395543 6.78526676976629e-06 -2.1277814514878 2.63898598170147e-08 miRanda -0.423737249967144 1.77998923967034e-09 NA NA NA hsa-miR-491-5p THBS1 0.615770352820862 0.0418295628761654 -2.1277814514878 2.63898598170147e-08 miRanda -0.325347677729002 8.67284344402748e-07 NA NA NA hsa-miR-500a-5p THBS1 1.72110365584658 3.0807246212659e-10 -2.1277814514878 2.63898598170147e-08 mirMAP -0.230571146840421 0.000634888611580494 NA NA NA hsa-miR-576-5p THBS1 2.20467023993664 3.4053475193793e-19 -2.1277814514878 2.63898598170147e-08 mirMAP -0.534982297177329 6.59187971715022e-14 NA NA NA hsa-miR-589-3p THBS1 2.51971841868825 2.39125940264107e-11 -2.1277814514878 2.63898598170147e-08 mirMAP -0.296840513596274 5.92807281377074e-08 NA NA NA hsa-miR-590-3p THBS1 2.59289455139753 1.03200520972022e-21 -2.1277814514878 2.63898598170147e-08 miRanda -0.503581788401113 2.48033554789491e-14 NA NA NA hsa-miR-590-5p THBS1 3.18300989862077 2.06813568597893e-26 -2.1277814514878 2.63898598170147e-08 miRanda;mirMAP -0.527120722441568 1.25825852850212e-19 NA NA NA hsa-miR-629-3p THBS1 2.36570947321815 1.33458201201618e-11 -2.1277814514878 2.63898598170147e-08 MirTarget -0.227068483079105 0.000123952249921696 NA NA NA hsa-miR-664a-3p THBS1 0.634721925861133 0.00520070178640421 -2.1277814514878 2.63898598170147e-08 mirMAP -0.570820389169422 2.01213211719281e-12 NA NA NA hsa-miR-671-5p THBS1 2.97733652243246 1.21148452344738e-23 -2.1277814514878 2.63898598170147e-08 MirTarget -0.50692748391382 1.23943837944331e-16 NA NA NA hsa-miR-7-1-3p THBS1 1.84950311431067 1.25300363397654e-13 -2.1277814514878 2.63898598170147e-08 MirTarget;mirMAP -0.527473744629066 1.61947893119384e-13 NA NA NA hsa-miR-92a-3p THBS1 2.06460102658973 1.44784291084757e-19 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate -0.769477829682195 9.27438286986465e-25 NA NA NA hsa-miR-940 THBS1 3.21011374578759 2.7923171128495e-14 -2.1277814514878 2.63898598170147e-08 MirTarget -0.39529960019763 2.55260567750224e-18 NA NA NA hsa-miR-944 THBS1 2.91186649218281 1.31574753528144e-06 -2.1277814514878 2.63898598170147e-08 MirTarget;PITA -0.199538618837297 1.63491480056503e-10 NA NA NA hsa-miR-98-5p THBS1 1.11154309866014 1.24143909012085e-08 -2.1277814514878 2.63898598170147e-08 miRNAWalker2_validate -0.724389356283188 5.99048052515149e-15 NA NA NA hsa-miR-340-5p TNFRSF10B 0.30282999308409 0.157744789519131 0.0854160195132305 0.697363353003698 miRNAWalker2_validate -0.130618058364656 0.00887614707841885 NA NA NA hsa-miR-222-3p TP53 0.851783756239071 0.00266783568486918 0.17313275059387 0.48779406985331 miRNAWalker2_validate -0.116904656132339 0.00589565055970469 NA NA NA hsa-let-7f-1-3p ZMAT3 1.55054488387679 7.31326775537952e-11 -0.834456180454181 0.000338643864818007 mirMAP -0.16946061848611 0.000412036501244573 NA NA NA hsa-miR-106a-5p ZMAT3 2.48882002962836 1.99757727374521e-12 -0.834456180454181 0.000338643864818007 mirMAP -0.142469467536183 4.02360691529699e-06 NA NA NA hsa-miR-106b-5p ZMAT3 2.46782146831112 1.36171495805632e-23 -0.834456180454181 0.000338643864818007 mirMAP -0.282265158645011 3.9032817146988e-11 NA NA NA hsa-miR-130a-3p ZMAT3 2.02021855058359 1.26427102520415e-11 -0.834456180454181 0.000338643864818007 MirTarget -0.275314037552642 2.07285921665374e-14 NA NA NA hsa-miR-130b-3p ZMAT3 3.5350292994509 1.73934183852569e-27 -0.834456180454181 0.000338643864818007 MirTarget -0.180797471768283 1.85048076646363e-08 NA NA NA hsa-miR-148a-3p ZMAT3 1.26728238710376 2.52325607402829e-06 -0.834456180454181 0.000338643864818007 MirTarget -0.15481806602257 0.000196098190067775 NA NA NA hsa-miR-148b-3p ZMAT3 1.97815018171325 6.07715327281595e-26 -0.834456180454181 0.000338643864818007 MirTarget -0.273482522289934 1.44857428128542e-06 NA NA NA hsa-miR-148b-5p ZMAT3 2.18024067300781 2.84770452413262e-14 -0.834456180454181 0.000338643864818007 MirTarget -0.240968449328689 1.52772354158217e-09 NA NA NA hsa-miR-15a-5p ZMAT3 2.34669284378787 1.69544424500258e-23 -0.834456180454181 0.000338643864818007 MirTarget -0.282225430062774 3.53809862866338e-10 NA NA NA hsa-miR-15b-3p ZMAT3 2.33847028227712 4.29662877950836e-19 -0.834456180454181 0.000338643864818007 mirMAP -0.168650270200408 4.41395344755827e-05 NA NA NA hsa-miR-15b-5p ZMAT3 1.57112623124278 9.14247846750597e-13 -0.834456180454181 0.000338643864818007 MirTarget -0.195701755709237 9.68276829876357e-05 NA NA NA hsa-miR-16-5p ZMAT3 1.75824877145857 1.68592440033108e-16 -0.834456180454181 0.000338643864818007 MirTarget -0.287554777526983 1.49712800088692e-08 NA NA NA hsa-miR-17-5p ZMAT3 3.2710598467455 9.93128340539455e-27 -0.834456180454181 0.000338643864818007 mirMAP -0.294308692417991 1.25268598150092e-18 NA NA NA hsa-miR-182-5p ZMAT3 3.53923166221814 5.07781258121574e-21 -0.834456180454181 0.000338643864818007 mirMAP -0.128544205330595 6.09119797515569e-06 NA NA NA hsa-miR-186-5p ZMAT3 1.47445398888668 3.0781726595413e-15 -0.834456180454181 0.000338643864818007 MirTarget -0.363958388556554 3.81028018787515e-10 NA NA NA hsa-miR-18a-3p ZMAT3 2.14813400940323 2.23460809936028e-06 -0.834456180454181 0.000338643864818007 MirTarget -0.194212329118884 2.10883399996197e-10 NA NA NA hsa-miR-192-5p ZMAT3 2.69002528723195 1.22990188194432e-16 -0.834456180454181 0.000338643864818007 miRNAWalker2_validate;MirTarget -0.157546279355689 2.22058715876206e-06 NA NA NA hsa-miR-193b-3p ZMAT3 2.25711804611899 4.1940744806631e-14 -0.834456180454181 0.000338643864818007 MirTarget -0.185909174812597 3.26118182591496e-07 NA NA NA hsa-miR-19a-3p ZMAT3 3.42309334186656 3.02671914353412e-28 -0.834456180454181 0.000338643864818007 MirTarget -0.312345952538807 6.42670635336776e-22 NA NA NA hsa-miR-19b-3p ZMAT3 2.49529833021187 1.57028265846699e-23 -0.834456180454181 0.000338643864818007 MirTarget -0.376233887009293 7.15580034367699e-20 NA NA NA hsa-miR-20a-5p ZMAT3 3.15577933619191 4.19753346442454e-25 -0.834456180454181 0.000338643864818007 mirMAP -0.312739485981805 8.53488676809551e-21 NA NA NA hsa-miR-20b-5p ZMAT3 2.07706563022469 1.10107236083433e-05 -0.834456180454181 0.000338643864818007 mirMAP -0.115306719611214 8.89961396576946e-07 NA NA NA hsa-miR-21-3p ZMAT3 2.19296355304364 4.63665722759779e-15 -0.834456180454181 0.000338643864818007 MirTarget -0.124064725614556 0.00153601083198072 NA NA NA hsa-miR-2110 ZMAT3 1.16625590651194 6.4784195289885e-05 -0.834456180454181 0.000338643864818007 MirTarget -0.155490967766465 0.000167736766648193 NA NA NA hsa-miR-29b-1-5p ZMAT3 1.55384379072167 4.76870585070176e-05 -0.834456180454181 0.000338643864818007 mirMAP -0.143401307684141 3.56225036534625e-06 NA NA NA hsa-miR-301a-3p ZMAT3 2.81024345929638 2.29784847962242e-17 -0.834456180454181 0.000338643864818007 MirTarget -0.253421020183989 6.04999872951255e-14 NA NA NA hsa-miR-32-3p ZMAT3 2.02436999565685 5.94091197945307e-09 -0.834456180454181 0.000338643864818007 mirMAP -0.166284216792824 4.39051475734724e-06 NA NA NA hsa-miR-320b ZMAT3 1.55692969039548 1.41071034251915e-08 -0.834456180454181 0.000338643864818007 miRanda;mirMAP -0.124185436267894 0.00224557425064878 NA NA NA hsa-miR-361-5p ZMAT3 0.971859117235239 5.30236555707088e-09 -0.834456180454181 0.000338643864818007 MirTarget;miRNATAP -0.18943830547704 0.00526695718636052 NA NA NA hsa-miR-362-3p ZMAT3 2.0756816987665 3.89026064579331e-12 -0.834456180454181 0.000338643864818007 MirTarget -0.204307065515127 6.04836677711665e-08 NA NA NA hsa-miR-362-5p ZMAT3 1.59303749908759 9.582675908797e-08 -0.834456180454181 0.000338643864818007 mirMAP -0.193232416366736 1.76411320409595e-07 NA NA NA hsa-miR-374a-3p ZMAT3 0.533309330504947 0.00192918429705142 -0.834456180454181 0.000338643864818007 MirTarget;miRNATAP -0.256410119133156 0.000110727364034862 NA NA NA hsa-miR-374a-5p ZMAT3 0.484615721953736 0.0042981729815149 -0.834456180454181 0.000338643864818007 mirMAP -0.186802486812566 0.00572208590053874 NA NA NA hsa-miR-421 ZMAT3 1.17791565395543 6.78526676976629e-06 -0.834456180454181 0.000338643864818007 mirMAP;miRNATAP -0.135291483247521 0.00167166854196482 NA NA NA hsa-miR-423-5p ZMAT3 0.963563145758616 9.86109015204915e-07 -0.834456180454181 0.000338643864818007 miRNATAP -0.172756201055426 0.00260117536869847 NA NA NA hsa-miR-424-5p ZMAT3 1.61930224569578 1.89642549274994e-10 -0.834456180454181 0.000338643864818007 MirTarget -0.119681671045599 0.00624935437583286 NA NA NA hsa-miR-425-5p ZMAT3 3.07071373939774 4.02947460586085e-25 -0.834456180454181 0.000338643864818007 MirTarget -0.224643009777132 2.0514161603342e-10 NA NA NA hsa-miR-454-3p ZMAT3 2.47234127820054 1.12878018736016e-21 -0.834456180454181 0.000338643864818007 MirTarget -0.363381591815153 1.19251759331658e-18 NA NA NA hsa-miR-491-5p ZMAT3 0.615770352820862 0.0418295628761654 -0.834456180454181 0.000338643864818007 MirTarget -0.134726615689825 0.000780823179377649 NA NA NA hsa-miR-501-5p ZMAT3 1.93654809410041 1.3779381780759e-10 -0.834456180454181 0.000338643864818007 MirTarget;mirMAP -0.201676766368906 6.95040686219654e-08 NA NA NA hsa-miR-550a-3p ZMAT3 1.78308792532884 2.47673569057582e-07 -0.834456180454181 0.000338643864818007 mirMAP -0.138536198215136 0.000149783740707612 NA NA NA hsa-miR-576-5p ZMAT3 2.20467023993664 3.4053475193793e-19 -0.834456180454181 0.000338643864818007 MirTarget -0.279423664262482 1.11388278609747e-10 NA NA NA hsa-miR-590-3p ZMAT3 2.59289455139753 1.03200520972022e-21 -0.834456180454181 0.000338643864818007 mirMAP;miRNATAP -0.268161432732507 2.1836414185913e-11 NA NA NA hsa-miR-590-5p ZMAT3 3.18300989862077 2.06813568597893e-26 -0.834456180454181 0.000338643864818007 miRanda -0.255744696795588 7.00838775165953e-13 NA NA NA hsa-miR-660-5p ZMAT3 1.82692095272076 1.92040083607196e-13 -0.834456180454181 0.000338643864818007 mirMAP -0.282692466132324 8.63511156073178e-11 NA NA NA hsa-miR-671-5p ZMAT3 2.97733652243246 1.21148452344738e-23 -0.834456180454181 0.000338643864818007 MirTarget -0.235272988406629 3.43074380188971e-10 NA NA NA hsa-miR-7-1-3p ZMAT3 1.84950311431067 1.25300363397654e-13 -0.834456180454181 0.000338643864818007 mirMAP -0.222019938505592 3.8971003385689e-07 NA NA NA hsa-miR-93-5p ZMAT3 3.03961860136032 1.11672209617395e-24 -0.834456180454181 0.000338643864818007 mirMAP -0.291819964533846 4.94330260498644e-17 NA NA NA hsa-miR-942-5p ZMAT3 2.35195531153712 5.45059325728841e-16 -0.834456180454181 0.000338643864818007 MirTarget -0.242145086874424 5.4132024232525e-11 NA NA NA