miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-23a-3p APAF1 1.10685926969665 3.16223452471768e-08 0.298695417693903 0.227685316305025 miRNATAP -0.491899186506395 3.94456341577364e-09 24992592;24249161 Luciferase assay was performed to verify a putative target site of miR-23a in the 3'-UTR of apoptosis protease activating factor 1 APAF1 mRNA; The expression levels of miR-23a and APAF1 in CRC cell lines SW480 and SW620 and clinical samples were assessed using reverse transcription-quantitative real-time PCR RT-qPCR and Western blot; Moreover miR-23a up-regulation was coupled with APAF1 down-regulation in CRC tissue samples;We found that the expression of miR-23a was increased and the level of apoptosis-activating factor-1 APAF-1 was decreased in 5-FU-treated colon cancer cells compared to untreated cells; APAF-1 as a target gene of miR-23a was identified and miR-23a antisense-induced increase in the activation of caspase-9 was observed ; colorectal cancer;colorectal cancer hsa-miR-23b-3p APAF1 -0.286465516491361 0.18664668472995 0.298695417693903 0.227685316305025 miRNATAP -0.541460697050492 5.01748351159025e-12 NA NA NA hsa-miR-27a-3p APAF1 1.29886998470345 2.68986118553486e-08 0.298695417693903 0.227685316305025 miRNATAP -0.390173318022615 4.7184175078605e-08 NA NA NA hsa-miR-27b-3p APAF1 0.0836323965901755 0.725273923274 0.298695417693903 0.227685316305025 miRNATAP -0.477647814500079 2.53647023848586e-11 NA NA NA hsa-miR-708-3p APAF1 0.623782703831307 0.275355868302311 0.298695417693903 0.227685316305025 mirMAP -0.239415264582338 2.50187362969727e-17 NA NA NA hsa-miR-944 APAF1 3.33123095809751 0.0177835044930391 0.298695417693903 0.227685316305025 miRNATAP -0.133026823389135 2.79320179778191e-24 NA NA NA hsa-miR-125a-3p ATM 1.19506542853853 0.00164364545717311 -0.394769053567885 0.0834135681853264 miRanda -0.132495092210333 0.00187961461837904 NA NA NA hsa-miR-203a-3p ATM 1.44736908507496 0.0394116388736904 -0.394769053567885 0.0834135681853264 MirTarget -0.138506783210379 2.82519981053352e-10 24145123;27542403 miR 203 induces oxaliplatin resistance in colorectal cancer cells by negatively regulating ATM kinase; In silico analysis identified ataxia telangiectasia mutated ATM a primary mediator of the DNA damage response as a potential target of miR-203; Using TCGA database we identified a significant reverse correlation of miR-203 and ATM expression in CRC tissues; We validated ATM as a bona fide target of miR-203 in CRC cells; Mutation of the putative miR-203 binding site in the 3' untranslated region 3'UTR of the ATM mRNA abolished the inhibitory effect of miR-203 on ATM;MiR 203 inhibits tumor invasion and metastasis in gastric cancer by ATM; Our results showed that miR-203 was significantly downregulated in gastric cancer tissues and cells while ataxia telangiectasia mutated kinase ATM was upregulated in gastric cancer tissues and cells and was directly regulated by miR-203; ATM knockdown phenocopied the effect of miR-203 overexpression drug resistance;metastasis colorectal cancer;gastric cancer hsa-miR-590-5p ATM 1.04171442093217 0.000272475544954865 -0.394769053567885 0.0834135681853264 mirMAP -0.169733213019369 0.00247037314325533 NA NA NA hsa-miR-30b-5p ATR -0.430720743745757 0.0593564057320791 0.73072161730785 0.000167590638966927 mirMAP -0.185879461945995 0.0026504272804509 NA NA NA hsa-miR-30a-5p BAX -1.71922855140016 3.20574349285256e-07 1.23402997413945 2.35105004724979e-11 miRNAWalker2_validate -0.14794228476148 0.000184560208965232 NA NA NA hsa-miR-365a-3p BAX -0.891537000455889 0.0025537650440002 1.23402997413945 2.35105004724979e-11 miRNAWalker2_validate -0.185079422057606 5.80881818302673e-05 24216611 MiR 365 induces gemcitabine resistance in pancreatic cancer cells by targeting the adaptor protein SHC1 and pro apoptotic regulator BAX drug resistance pancreatic cancer hsa-let-7a-2-3p BBC3 0.195895719455896 0.643556335039042 1.16438832735563 0.000138050719155024 MirTarget -0.189649631349489 0.000323992880583244 NA NA NA hsa-miR-149-5p BBC3 0.706277554734069 0.296848731347677 1.16438832735563 0.000138050719155024 miRNATAP -0.12753681248736 7.31829046434304e-05 NA NA NA hsa-miR-27b-3p BBC3 0.0836323965901755 0.725273923274 1.16438832735563 0.000138050719155024 miRNATAP -0.254737303455527 0.00692776633122051 NA NA NA hsa-miR-129-5p CASP8 -2.56914016541176 2.36237896095709e-06 1.08073884606891 5.24067403889636e-07 miRanda -0.14203060790996 3.69274761688692e-07 NA NA NA hsa-miR-143-3p CASP8 -0.661937718854688 0.0483213962135842 1.08073884606891 5.24067403889636e-07 MirTarget -0.193498599025905 3.01762109778414e-05 NA NA NA hsa-miR-193b-3p CASP9 0.281752012268679 0.451258902263119 -0.314756842171161 0.110040278687801 miRNAWalker2_validate -0.173981251979618 2.15802379337291e-06 NA NA NA hsa-miR-139-5p CCNB1 -1.83374360776637 1.22019901002824e-09 2.86029513012046 1.09631454385186e-20 miRanda -0.494002119509298 2.06388587911453e-11 NA NA NA hsa-miR-338-3p CCND1 -0.959276231066179 0.0191473351014091 0.62464034212269 0.131198685408586 miRNAWalker2_validate;miRTarBase;miRanda -0.237799286777961 0.000859583563159798 NA NA NA hsa-let-7a-3p CCND2 0.503048155002889 0.0411145454335299 -0.495732246530821 0.299995013548082 mirMAP -0.539007488034469 9.58303749078928e-05 20418948 MicroRNA let 7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2 prostate cancer hsa-let-7b-3p CCND2 0.21838629112113 0.296038413622566 -0.495732246530821 0.299995013548082 mirMAP -0.458425126620734 0.00587678366196967 NA NA NA hsa-miR-106b-5p CCND2 1.70863907461894 2.04074794107142e-08 -0.495732246530821 0.299995013548082 miRNAWalker2_validate;miRTarBase;miRNATAP -0.420111135029172 8.79445971678554e-05 NA NA NA hsa-miR-130b-5p CCND2 0.701350393759937 0.0510142718395002 -0.495732246530821 0.299995013548082 mirMAP -0.393986053261913 2.48361314637284e-05 NA NA NA hsa-miR-141-3p CCND2 1.45548825076434 0.00115958850961764 -0.495732246530821 0.299995013548082 MirTarget;TargetScan -0.213829265059233 0.00435932519246045 NA NA NA hsa-miR-15b-5p CCND2 1.62046407540723 5.42152621571078e-09 -0.495732246530821 0.299995013548082 miRNATAP -0.436574112059407 0.000206222006606723 NA NA NA hsa-miR-16-2-3p CCND2 1.8048179609245 2.83095715136409e-09 -0.495732246530821 0.299995013548082 mirMAP -0.54923627359894 1.83952504317926e-07 NA NA NA hsa-miR-16-5p CCND2 1.00515772948091 5.33912942007619e-06 -0.495732246530821 0.299995013548082 miRNAWalker2_validate;miRNATAP -0.666404634405191 9.74095569654959e-06 NA NA NA hsa-miR-181a-2-3p CCND2 0.901732254836667 0.000830304681396734 -0.495732246530821 0.299995013548082 mirMAP -0.381747755391939 0.00236274066260059 NA NA NA hsa-miR-182-5p CCND2 0.887369486030646 0.0310640781418789 -0.495732246530821 0.299995013548082 miRNAWalker2_validate;miRTarBase;miRNATAP -0.320494097434122 8.56279904949731e-05 NA NA NA hsa-miR-183-5p CCND2 1.65900927871187 0.000522025364980187 -0.495732246530821 0.299995013548082 miRNATAP -0.293018901580787 2.29962975856133e-05 NA NA NA hsa-miR-19b-3p CCND2 0.759818275708988 0.00652779573186786 -0.495732246530821 0.299995013548082 miRNAWalker2_validate;MirTarget;miRNATAP -0.321137891620492 0.0085283453454723 NA NA NA hsa-miR-21-3p CCND2 1.75245546017565 2.36505176715025e-10 -0.495732246530821 0.299995013548082 mirMAP -0.330852598235875 0.00510140773909108 NA NA NA hsa-miR-224-3p CCND2 1.52422171450716 0.00650463542340912 -0.495732246530821 0.299995013548082 mirMAP -0.268591207673576 3.7771965471366e-05 NA NA NA hsa-miR-26b-5p CCND2 -0.301646326928219 0.160081421968103 -0.495732246530821 0.299995013548082 mirMAP;miRNATAP -0.575426727790569 0.00033543015515606 NA NA NA hsa-miR-301a-3p CCND2 1.44721900431161 6.35068481036652e-06 -0.495732246530821 0.299995013548082 miRNAWalker2_validate -0.383757128016972 0.0002041077399091 NA NA NA hsa-miR-3065-3p CCND2 -1.03551124329864 0.0218397494946288 -0.495732246530821 0.299995013548082 MirTarget;miRNATAP -0.202763155868079 0.00685463393219998 NA NA NA hsa-miR-3065-5p CCND2 -0.241623656423063 0.633116553078159 -0.495732246530821 0.299995013548082 mirMAP -0.236172852888811 0.000570437819651464 NA NA NA hsa-miR-424-5p CCND2 1.08509868581983 0.000420473853818268 -0.495732246530821 0.299995013548082 miRNATAP -0.393741408465626 0.000302491529945387 NA NA NA hsa-miR-429 CCND2 1.40182602301928 0.00900426780286475 -0.495732246530821 0.299995013548082 miRNATAP -0.193697975832347 0.00200087926732012 NA NA NA hsa-miR-450b-5p CCND2 0.46157963033014 0.13273722727895 -0.495732246530821 0.299995013548082 MirTarget;PITA;miRNATAP -0.319300360536976 0.00413356428949538 NA NA NA hsa-miR-590-3p CCND2 1.11654983899947 0.000160782154924864 -0.495732246530821 0.299995013548082 miRanda;mirMAP -0.332566390160307 0.00348233876262983 NA NA NA hsa-miR-590-5p CCND2 1.04171442093217 0.000272475544954865 -0.495732246530821 0.299995013548082 mirMAP -0.40586994965179 0.000547583893303904 NA NA NA hsa-miR-9-3p CCND2 0.330346058941473 0.541111939982661 -0.495732246530821 0.299995013548082 MirTarget;mirMAP;miRNATAP -0.190791407012111 0.00360684983294818 NA NA NA hsa-miR-93-5p CCND2 1.7515174434778 1.59386482413883e-08 -0.495732246530821 0.299995013548082 miRNATAP -0.321341054229317 0.00244079386586045 NA NA NA hsa-miR-96-5p CCND2 1.14243043653372 0.00942969392739105 -0.495732246530821 0.299995013548082 TargetScan;miRNATAP -0.334672732851839 2.58915737933556e-05 NA NA NA hsa-miR-96-5p CCND3 1.14243043653372 0.00942969392739105 0.0356607593991125 0.883520357560836 TargetScan -0.119818202566509 0.00327749541512401 NA NA NA hsa-miR-125b-5p CCNE1 -0.509303501421334 0.133265526313161 2.19321666957752 7.58574979891593e-09 miRNAWalker2_validate -0.26234320685223 0.00155046398906082 NA NA NA hsa-miR-195-5p CCNE1 -0.907998841926297 0.00150511564650591 2.19321666957752 7.58574979891593e-09 miRNAWalker2_validate;MirTarget;miRNATAP -0.431213874492418 7.80520066718325e-06 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-497-5p CCNE1 -0.801030912431774 0.00360386743876612 2.19321666957752 7.58574979891593e-09 MirTarget;miRNATAP -0.428398960662757 2.16247264707644e-05 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-26a-5p CCNE2 -0.384110714440647 0.0442498636014044 1.35587893076287 2.63917326776567e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.435562646712902 9.72974519873178e-05 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-30a-5p CCNE2 -1.71922855140016 3.20574349285256e-07 1.35587893076287 2.63917326776567e-06 miRNATAP -0.216731893468082 0.000339344793439292 NA NA NA hsa-let-7b-5p CCNG1 0.0582902155815681 0.781400138984745 -1.13108882371499 9.86710296926288e-07 miRNAWalker2_validate -0.282605727233217 0.000538677443074175 NA NA NA hsa-miR-1271-5p CCNG1 1.15538250415776 0.00207865862606828 -1.13108882371499 9.86710296926288e-07 MirTarget -0.178721964392492 6.35114520194081e-05 26477861 MiR 1271 Inhibits Ovarian Cancer Growth by Targeting Cyclin G1; MiR-1271 exerted its role by targeting CCNG1 ovarian cancer hsa-miR-21-5p CCNG1 1.75445050487988 2.41825549682022e-23 -1.13108882371499 9.86710296926288e-07 miRNAWalker2_validate -0.347301071779302 5.50974764199418e-05 NA NA NA hsa-miR-23a-3p CCNG1 1.10685926969665 3.16223452471768e-08 -1.13108882371499 9.86710296926288e-07 MirTarget;miRNATAP -0.532259896499842 1.78154816731613e-11 NA NA NA hsa-miR-23b-3p CCNG1 -0.286465516491361 0.18664668472995 -1.13108882371499 9.86710296926288e-07 MirTarget;miRNATAP -0.231629867164542 0.00341126044931762 26872615 MiR 23b targets cyclin G1 and suppresses ovarian cancer tumorigenesis and progression; Dual-luciferase reporter assay and a xenograft mouse model were used to examine the expression of miR-23b and its target gene CCNG1; Dual-luciferase reporter assay showed that miR-23b bound with the 3' untranslated region of CCNG1; Furthermore miR-23b inhibited tumor growth and suppressed CCNG1 expression in vitro; Our findings show that miR-23b may inhibit ovarian cancer tumorigenesis and progression by downregulating CCNG1 and the expression of the relevant genes progression;tumorigenesis ovarian cancer hsa-miR-24-3p CCNG1 1.08578167539644 6.18549359075268e-07 -1.13108882371499 9.86710296926288e-07 miRNAWalker2_validate -0.45887696015485 4.33419272791538e-10 NA NA NA hsa-miR-27a-3p CCNG1 1.29886998470345 2.68986118553486e-08 -1.13108882371499 9.86710296926288e-07 MirTarget;miRNATAP -0.442359287991954 5.46951347639654e-11 NA NA NA hsa-miR-27b-3p CCNG1 0.0836323965901755 0.725273923274 -1.13108882371499 9.86710296926288e-07 MirTarget;miRNATAP -0.284631422176409 6.49924743751419e-05 26623719 Moreover miR-27b directly targets the 3' untranslated regions 3'-UTRs of CCNG1 a well-known negative regulator of P53 stability; Interestingly miR-27b up-regulation leads to increased miR-508-5p expression and this phenomenon is mediated by CCNG1 and P53 gastric cancer hsa-miR-299-5p CCNG1 -0.173288988251251 0.638938431622539 -1.13108882371499 9.86710296926288e-07 MirTarget -0.18297274099047 7.26774175689903e-05 NA NA NA hsa-miR-409-3p CCNG1 0.648757698056077 0.0515133779537138 -1.13108882371499 9.86710296926288e-07 MirTarget;PITA;miRNATAP -0.158586242459603 0.00169881416912309 NA NA NA hsa-let-7f-1-3p CCNG2 1.28704406797397 1.17954701106157e-06 -0.203226208630122 0.447743790900586 MirTarget;mirMAP -0.280583185411179 5.54442281651905e-05 NA NA NA hsa-miR-503-5p CCNG2 1.88558728489534 2.26317868368257e-06 -0.203226208630122 0.447743790900586 miRNAWalker2_validate -0.166384224972927 0.000304933543905685 NA NA NA hsa-miR-145-5p CDK4 -1.75133578902314 1.54965572197748e-05 1.15953651250859 6.32013674437628e-08 miRNAWalker2_validate;miRTarBase -0.128867203591511 0.00065492290615456 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-195-5p CDK4 -0.907998841926297 0.00150511564650591 1.15953651250859 6.32013674437628e-08 miRNAWalker2_validate;miRTarBase -0.160202666019046 0.00343668366819135 NA NA NA hsa-miR-101-3p CDK6 -0.451263906815861 0.0283387250173031 0.299248876757035 0.400797488709762 mirMAP -0.390880566201796 0.0017319791753036 NA NA NA hsa-miR-129-5p CDK6 -2.56914016541176 2.36237896095709e-06 0.299248876757035 0.400797488709762 miRNAWalker2_validate -0.131377486244208 0.00426734981945999 24055727 Interestingly we showed that cyclin dependent kinase 6 CDK6 a cell cycle-associated protein involved in G1-S transition was a target of miR-129 gastric cancer hsa-miR-30d-3p CDK6 -0.545113326540761 0.0433733076874155 0.299248876757035 0.400797488709762 mirMAP -0.279904118765617 0.00297854403213622 NA NA NA hsa-miR-30d-5p CDK6 -0.5492586056147 0.0140125082460125 0.299248876757035 0.400797488709762 mirMAP -0.442605862369401 9.48761181124052e-05 NA NA NA hsa-let-7d-5p CDKN1A 0.981733854788227 7.26366577706469e-07 -0.527054376093461 0.0994835075201721 MirTarget -0.396477553702672 0.000479683524112273 NA NA NA hsa-let-7g-5p CDKN1A 0.325297685027927 0.241142963826631 -0.527054376093461 0.0994835075201721 MirTarget -0.314745562281535 0.000125688268144105 NA NA NA hsa-let-7i-5p CDKN1A 0.798954594465461 2.99735653959449e-05 -0.527054376093461 0.0994835075201721 MirTarget -0.322641868184451 0.00701233652117083 NA NA NA hsa-miR-101-3p CDKN1A -0.451263906815861 0.0283387250173031 -0.527054376093461 0.0994835075201721 MirTarget -0.447896067740702 5.80221830113762e-05 NA NA NA hsa-miR-106b-5p CDKN1A 1.70863907461894 2.04074794107142e-08 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.319691088785281 7.24575207668053e-06 NA NA NA hsa-miR-146a-5p CDKN1A 1.82051649415508 2.03651725047679e-05 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate -0.181963416651094 0.000452608950605388 NA NA NA hsa-miR-17-5p CDKN1A 1.65862087862483 8.45603406215941e-08 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.261784540083651 0.000223281598813828 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-20a-5p CDKN1A 1.4503375861709 5.7813456424762e-07 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.31050980918024 4.24749867032784e-05 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-30b-3p CDKN1A -0.266398938200332 0.400847813143994 -0.527054376093461 0.0994835075201721 MirTarget -0.232429451010355 0.00125169502954217 NA NA NA hsa-miR-335-5p CDKN1A 1.59953351087307 5.56170899867314e-05 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate -0.323092043513641 2.937052662711e-09 NA NA NA hsa-miR-345-5p CDKN1A 1.83686164833727 1.1036889735857e-08 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate;miRTarBase;MirTarget -0.244264832927155 0.000326167065691554 NA NA NA hsa-miR-423-3p CDKN1A 0.817576939524592 0.000261533454265696 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate;miRTarBase -0.324900447056002 0.00137746661266851 NA NA NA hsa-miR-423-5p CDKN1A 0.832613878736338 0.000791321227103338 -0.527054376093461 0.0994835075201721 MirTarget -0.413612288167575 4.59123064989195e-06 NA NA NA hsa-miR-429 CDKN1A 1.40182602301928 0.00900426780286475 -0.527054376093461 0.0994835075201721 miRNATAP -0.134914410282826 0.00128436889666203 NA NA NA hsa-miR-93-5p CDKN1A 1.7515174434778 1.59386482413883e-08 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.319008304670668 5.03968506845812e-06 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 1.90585138008274 3.11903311044863e-09 -0.527054376093461 0.0994835075201721 miRNAWalker2_validate -0.183830567960007 0.00704444870897519 NA NA NA hsa-miR-125b-5p CDKN2A -0.509303501421334 0.133265526313161 1.06173069875751 0.261325871405663 miRNAWalker2_validate -0.581163651183482 0.00347064875298016 23585871 In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b; Conversely treatment of prostate cancer cells with an inhibitor of miR-125b anti-miR-125b resulted in increased expression of p14ARF decreased level of Mdm2 and induction of apoptosis; In addition overexpression of miR-125b in p53-deficient PC3 cells induced down-regulation of p14ARF which leads to increased cell proliferation through a p53-independent manner prostate cancer hsa-miR-365a-3p CDKN2A -0.891537000455889 0.0025537650440002 1.06173069875751 0.261325871405663 MirTarget -0.763276937785335 0.000738553773920597 NA NA NA hsa-miR-129-5p CHEK1 -2.56914016541176 2.36237896095709e-06 2.15613840663813 4.74725801073545e-17 miRanda -0.135365909159199 0.000158499471421881 NA NA NA hsa-miR-139-5p CHEK1 -1.83374360776637 1.22019901002824e-09 2.15613840663813 4.74725801073545e-17 miRanda -0.385896206112722 2.21600376918745e-10 NA NA NA hsa-miR-195-5p CHEK1 -0.907998841926297 0.00150511564650591 2.15613840663813 4.74725801073545e-17 MirTarget;miRNATAP -0.337334324952082 6.44181424303598e-07 25840419 MiR 195 suppresses non small cell lung cancer by targeting CHEK1; We discovered that CHEK1 was a direct target of miR-195 which decreased CHEK1 expression in lung cancer cells lung squamous cell cancer hsa-miR-497-5p CHEK1 -0.801030912431774 0.00360386743876612 2.15613840663813 4.74725801073545e-17 MirTarget;miRNATAP -0.345700480248046 1.03574513533624e-06 24464213 Checkpoint kinase 1 is negatively regulated by miR 497 in hepatocellular carcinoma; In silico analysis showed that CHEK1 was a candidate target of miR-497 which was previously found to be downregulated in HCC by us; To test whether miR-497 could bind to 3'untranslated region 3'UTR of CHEK1 luciferase reporter assay was conducted; The result revealed that miR-497 could bind to the 3'untranslated region 3'UTR of CHEK1 mRNA; Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1; Finally miR-497 expression was measured in the same 30 HCC samples and the correlation between miR-497 and CHEK1 was analyzed; The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1; Taken together these results demonstrated that CHEK1 was negatively regulated by miR-497 and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC which provided a potential molecular target for HCC therapy liver cancer hsa-miR-342-3p CYCS 0.318119432680668 0.269150960124243 0.195262771266198 0.359129576262205 mirMAP -0.206285282933732 7.83163671388272e-05 NA NA NA hsa-miR-326 DDB2 -0.429748586406214 0.2629894124021 0.671712983773986 0.00377665655342995 miRanda -0.178342194650347 3.72457884683347e-05 NA NA NA hsa-miR-106a-5p FAS -0.173255688252641 0.642872703263671 0.657268335933371 0.0207009292820455 miRNAWalker2_validate;miRTarBase -0.15547134509945 0.00429149494899305 22431000;27142596 miR 106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS; Bioinformatic analysis combining with validation experiments identified FAS as a direct target of miR-106a; Moreover a significant inverse correlation was found between miR-106a and FAS expression not only in gastric cancer cell lines but also in gastric cancer specimens; Taken together these findings suggest that ectopicly overexpressed miR-106a may play an oncogenic role in gastric carcinogenesis and impair extrinsic apoptotic pathway through targeting FAS;Functional experiment ascertained that miR-106a interacted with FAS and mediated caspase3 pathway tumorigenesis; gastric cancer;gastric cancer hsa-miR-129-5p FAS -2.56914016541176 2.36237896095709e-06 0.657268335933371 0.0207009292820455 miRanda -0.124419506861282 0.000695154070004086 NA NA NA hsa-miR-148b-3p GADD45A 0.295175884456199 0.17466422420702 -0.744381186012481 0.00247182040910746 MirTarget -0.214957101032821 0.00927854827400905 NA NA NA hsa-miR-454-3p GADD45A 0.625199142970745 0.0134890997402741 -0.744381186012481 0.00247182040910746 MirTarget;miRNATAP -0.217978566953222 0.00180852644098547 NA NA NA hsa-miR-1976 GADD45B 0.923172654386147 0.000118514313506673 -1.91754262778042 5.17429057228523e-08 miRNATAP -0.58594227558951 2.17579979542256e-08 NA NA NA hsa-miR-324-3p GADD45B 1.04940352369965 0.000387716796520961 -1.91754262778042 5.17429057228523e-08 MirTarget;miRNATAP -0.3687003729794 1.78715342431653e-05 NA NA NA hsa-miR-590-3p GADD45B 1.11654983899947 0.000160782154924864 -1.91754262778042 5.17429057228523e-08 miRanda -0.368023929046783 1.76170990966765e-05 NA NA NA hsa-miR-296-5p GADD45G 0.932146077883618 0.0602370559607489 -1.30721241002926 0.000929668878156123 miRNATAP -0.168728470747373 0.00456954912760057 NA NA NA hsa-miR-139-5p GTSE1 -1.83374360776637 1.22019901002824e-09 3.3547502176725 7.2373613282175e-28 miRanda -0.455696081652976 4.28942760532908e-09 NA NA NA hsa-let-7a-3p IGF1 0.503048155002889 0.0411145454335299 -2.08273193234781 0.00135003721777342 mirMAP -0.548762647030196 0.00421892046856383 NA NA NA hsa-let-7b-3p IGF1 0.21838629112113 0.296038413622566 -2.08273193234781 0.00135003721777342 mirMAP -0.635547835878874 0.00549026374201425 NA NA NA hsa-let-7f-1-3p IGF1 1.28704406797397 1.17954701106157e-06 -2.08273193234781 0.00135003721777342 mirMAP -0.851363397411719 5.26675622894171e-07 NA NA NA hsa-miR-103a-2-5p IGF1 0.808041560899298 0.019989162317947 -2.08273193234781 0.00135003721777342 mirMAP -0.553669997923913 3.26136051419373e-05 NA NA NA hsa-miR-130b-3p IGF1 1.32714007211004 4.78676252285191e-05 -2.08273193234781 0.00135003721777342 MirTarget -0.899465743270437 2.58112149375995e-11 NA NA NA hsa-miR-15b-3p IGF1 1.75738933306437 3.00554466232543e-08 -2.08273193234781 0.00135003721777342 mirMAP -0.968826693204176 7.96214227786153e-13 NA NA NA hsa-miR-16-1-3p IGF1 1.43227522002564 3.70920454953168e-08 -2.08273193234781 0.00135003721777342 mirMAP -0.890729087705412 1.88566016831073e-07 NA NA NA hsa-miR-181b-5p IGF1 1.64216500592669 1.89228845300725e-08 -2.08273193234781 0.00135003721777342 mirMAP -0.484722871486351 0.00175444902634641 NA NA NA hsa-miR-186-5p IGF1 0.149964130725901 0.434709542998771 -2.08273193234781 0.00135003721777342 mirMAP -0.854024244188329 0.000614672571310158 NA NA NA hsa-miR-19a-3p IGF1 1.26669687448473 0.000105972838514412 -2.08273193234781 0.00135003721777342 MirTarget -0.774005120681808 1.66575604220791e-08 NA NA NA hsa-miR-19b-1-5p IGF1 1.38738648038895 1.3113681938066e-06 -2.08273193234781 0.00135003721777342 mirMAP -0.926821468239418 1.88689070400957e-09 NA NA NA hsa-miR-19b-3p IGF1 0.759818275708988 0.00652779573186786 -2.08273193234781 0.00135003721777342 MirTarget -0.817907622748148 6.24186432835086e-07 NA NA NA hsa-miR-20a-3p IGF1 1.13957127410163 0.000451087640268489 -2.08273193234781 0.00135003721777342 mirMAP -0.98528355307319 2.65793420646273e-13 NA NA NA hsa-miR-27a-3p IGF1 1.29886998470345 2.68986118553486e-08 -2.08273193234781 0.00135003721777342 miRNAWalker2_validate;miRTarBase -0.616931404496154 0.00158345718629553 NA NA NA hsa-miR-301a-3p IGF1 1.44721900431161 6.35068481036652e-06 -2.08273193234781 0.00135003721777342 MirTarget -0.441884252991246 0.00198755452375828 NA NA NA hsa-miR-301b-3p IGF1 1.10080095582241 0.0715568440482997 -2.08273193234781 0.00135003721777342 MirTarget -0.489626457605712 1.80285281431259e-06 NA NA NA hsa-miR-3065-5p IGF1 -0.241623656423063 0.633116553078159 -2.08273193234781 0.00135003721777342 mirMAP -0.280592172239543 0.00303638700940348 NA NA NA hsa-miR-32-3p IGF1 0.583525764322974 0.11837203008551 -2.08273193234781 0.00135003721777342 mirMAP -0.526531906386405 0.000169656014507106 NA NA NA hsa-miR-320a IGF1 0.593888711838028 0.011897419390526 -2.08273193234781 0.00135003721777342 miRNATAP -0.565003176356916 0.00470570622795822 NA NA NA hsa-miR-320b IGF1 1.11183660885284 0.000497971211648503 -2.08273193234781 0.00135003721777342 miRNATAP -0.636268160611304 8.75874676911532e-06 NA NA NA hsa-miR-320c IGF1 0.455072851945474 0.240613785444485 -2.08273193234781 0.00135003721777342 miRNATAP -0.416076292118249 0.00294415535123176 NA NA NA hsa-miR-33a-3p IGF1 0.347011402017138 0.321709379745392 -2.08273193234781 0.00135003721777342 MirTarget -0.68230953278373 8.05008237434513e-07 NA NA NA hsa-miR-3662 IGF1 1.82780708047676 0.0054036501346178 -2.08273193234781 0.00135003721777342 MirTarget -0.445685489349264 6.10879019924172e-07 NA NA NA hsa-miR-421 IGF1 1.80720854070385 1.59709651202848e-07 -2.08273193234781 0.00135003721777342 PITA -0.446185670355332 0.000667970192973034 NA NA NA hsa-miR-454-3p IGF1 0.625199142970745 0.0134890997402741 -2.08273193234781 0.00135003721777342 MirTarget -0.54504877697133 0.00336959864095608 NA NA NA hsa-miR-486-5p IGF1 -0.627607241830911 0.155274583521138 -2.08273193234781 0.00135003721777342 PITA;miRNATAP -0.376922293558939 0.000339259187750958 NA NA NA hsa-miR-576-5p IGF1 0.625714137488957 0.0417100301848969 -2.08273193234781 0.00135003721777342 PITA;mirMAP;miRNATAP -0.758998498936523 3.66567723101284e-07 NA NA NA hsa-miR-577 IGF1 0.911619788986754 0.2256062124148 -2.08273193234781 0.00135003721777342 PITA -0.356762409648375 6.35759889251154e-09 NA NA NA hsa-miR-590-3p IGF1 1.11654983899947 0.000160782154924864 -2.08273193234781 0.00135003721777342 MirTarget;miRanda;mirMAP;miRNATAP -0.61013399064959 8.43840390366035e-05 NA NA NA hsa-miR-592 IGF1 0.779567202789068 0.204498177014474 -2.08273193234781 0.00135003721777342 mirMAP -0.405253750267835 3.79197293554194e-07 NA NA NA hsa-miR-629-5p IGF1 1.06377074768516 0.000536624707722274 -2.08273193234781 0.00135003721777342 mirMAP -0.88383980266392 1.46631776640132e-09 NA NA NA hsa-miR-940 IGF1 1.64406174271686 0.000690854900107004 -2.08273193234781 0.00135003721777342 MirTarget;PITA;miRNATAP -0.451608062622453 5.12340787556199e-05 NA NA NA hsa-miR-143-3p MDM2 -0.661937718854688 0.0483213962135842 0.0953337413007871 0.725153770779448 miRNAWalker2_validate -0.192980048252097 0.000726275884433861 NA NA NA hsa-let-7b-5p MDM4 0.0582902155815681 0.781400138984745 -0.425067233161641 0.0212044086569621 miRNAWalker2_validate;MirTarget -0.164640164808616 0.00994954384671392 NA NA NA hsa-miR-127-5p MDM4 0.684144175589776 0.021634535736883 -0.425067233161641 0.0212044086569621 mirMAP -0.128903892846204 0.00340233442367969 NA NA NA hsa-miR-152-3p MDM4 0.436002345388935 0.161701199884702 -0.425067233161641 0.0212044086569621 MirTarget -0.197617946771021 1.86589445279121e-06 NA NA NA hsa-miR-22-5p MDM4 0.804348748654247 0.000804065112767412 -0.425067233161641 0.0212044086569621 MirTarget -0.168752418097852 0.00196903525532538 NA NA NA hsa-miR-27a-3p MDM4 1.29886998470345 2.68986118553486e-08 -0.425067233161641 0.0212044086569621 miRNATAP -0.316520025272498 2.15138047169272e-09 NA NA NA hsa-miR-27b-3p MDM4 0.0836323965901755 0.725273923274 -0.425067233161641 0.0212044086569621 miRNATAP -0.223370154043439 5.4259480810411e-05 NA NA NA hsa-miR-126-5p PERP 0.41765430251855 0.0753161203651532 1.62178161809411 0.00158092259269313 mirMAP -0.423363169053219 0.00796432849901375 NA NA NA hsa-miR-150-5p PERP 0.147461377429675 0.753719621305461 1.62178161809411 0.00158092259269313 MirTarget -0.222723844734315 0.00462027179567768 NA NA NA hsa-miR-192-5p PERP 0.0809146624034405 0.941057093528709 1.62178161809411 0.00158092259269313 miRNAWalker2_validate -0.281416045659929 3.31179362705267e-20 NA NA NA hsa-miR-215-5p PERP 0.715250240418615 0.573784317311889 1.62178161809411 0.00158092259269313 miRNAWalker2_validate -0.218857387157125 3.89316828222827e-16 NA NA NA hsa-miR-29b-1-5p PERP 0.606560155953522 0.116355599574769 1.62178161809411 0.00158092259269313 MirTarget -0.249973839975087 0.00925475684452676 NA NA NA hsa-miR-362-3p PERP -0.0349414695893027 0.913780708043777 1.62178161809411 0.00158092259269313 miRanda -0.458152126254415 6.17272553440568e-05 NA NA NA hsa-miR-577 PERP 0.911619788986754 0.2256062124148 1.62178161809411 0.00158092259269313 PITA -0.215724391795287 1.26210944747506e-05 NA NA NA hsa-miR-660-5p PERP -0.0206552819434673 0.938868734063643 1.62178161809411 0.00158092259269313 MirTarget -0.924730840619218 2.19935759572714e-12 NA NA NA hsa-let-7a-5p PMAIP1 0.287487047056922 0.163415962187584 1.65135663448277 2.37072481035608e-06 TargetScan -0.332196991891857 0.00872833127861153 NA NA NA hsa-miR-200b-3p PMAIP1 0.974577911407732 0.0595020056914543 1.65135663448277 2.37072481035608e-06 MirTarget;TargetScan -0.154737198725253 0.00160298412925668 NA NA NA hsa-miR-375 PMAIP1 -2.94506033785642 0.00570147601654791 1.65135663448277 2.37072481035608e-06 miRanda -0.174675684723302 2.65908002402438e-15 NA NA NA hsa-miR-577 PMAIP1 0.911619788986754 0.2256062124148 1.65135663448277 2.37072481035608e-06 mirMAP -0.101676220276918 0.00332234214688593 NA NA NA hsa-miR-7-1-3p PMAIP1 0.709309110750947 0.0412301737463194 1.65135663448277 2.37072481035608e-06 mirMAP -0.26665816231333 0.000259274413143692 NA NA NA hsa-let-7a-3p PPM1D 0.503048155002889 0.0411145454335299 -0.0103806915783968 0.945672859328565 MirTarget -0.15523566881754 0.000367404277235878 NA NA NA hsa-let-7b-3p PPM1D 0.21838629112113 0.296038413622566 -0.0103806915783968 0.945672859328565 MirTarget -0.194869893563825 0.000172375876892711 NA NA NA hsa-let-7f-1-3p PPM1D 1.28704406797397 1.17954701106157e-06 -0.0103806915783968 0.945672859328565 MirTarget -0.105532379606087 0.00784735108314564 NA NA NA hsa-miR-103a-3p PTEN 0.840525139266028 5.83536475789158e-07 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase -0.257442252482047 3.11707848254512e-05 26511107;24828205 LncRNA GAS5 induces PTEN expression through inhibiting miR 103 in endometrial cancer cells; To investigate the expression of GAS5 PTEN and miR-103 RT-PCR was performed; Finally we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells; Through inhibiting the expression of miR-103 GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis and thus could be an important mediator in the pathogenesis of endometrial cancer;Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN ; endometrial cancer;colorectal cancer hsa-miR-106b-5p PTEN 1.70863907461894 2.04074794107142e-08 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase;miRNATAP -0.119092505372708 0.000319006222689429 24842611;26238857;26722252 MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance;Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells cell migration;drug resistance; gastric cancer;colorectal cancer;breast cancer hsa-miR-16-1-3p PTEN 1.43227522002564 3.70920454953168e-08 -0.439862628350054 0.00288389874110071 MirTarget -0.117398669841475 0.0027231630162008 NA NA NA hsa-miR-17-5p PTEN 1.65862087862483 8.45603406215941e-08 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.124323874557627 0.000136392655221086 27400681;23391506;23133552;26629823;24462867;26318586;26215320;25634356;26500892;24912422;23418359 GFRα2 prompts cell growth and chemoresistance through down regulating tumor suppressor gene PTEN via Mir 17 5p in pancreatic cancer; Mechanically we discovered that high GFRα2 expression level leads to PTEN inactivation via enhancing Mir-17-5p level;We found that these phenotypes were the results of miR-17 targeting PTEN;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;We hypothesized that knocking down the oncogenic microRNA oncomiR miR-17-5p might restore the expression levels of PDCD4 and PTEN tumor suppressor proteins illustrating a route to oligonucleotide therapy of TNBC; Contrary to conventional wisdom antisense knockdown of oncomiR miR-17-5p guide strand reduced PDCD4 and PTEN proteins by 1.8±0.3 fold in human TNBC cells instead of raising them; Bioinformatics analysis and folding energy calculations revealed that mRNA targets of miR-17-5p guide strand such as PDCD4 and PTEN could also be regulated by miR-17-3p passenger strand;miR 17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression; Expression of miR-17 was negatively correlated with PTEN in OS tissues;Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR 17 family in prostate cancer; Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo;In addition ERβ expression significantly increased in calycosin-treated HCT-116 cells followed by a decrease of miR-17 and up-regulation of PTEN; Our results indicate that calycosin has an inhibitory effect on CRC which might be obtained by ERβ-mediated regulation of miR-17 and PTEN expression;The High Expression of the microRNA 17 92 Cluster and its Paralogs and the Downregulation of the Target Gene PTEN Is Associated with Primary Cutaneous B Cell Lymphoma Progression;MicroRNA 17 5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling; miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells;MicroRNA 17 5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression; We found that PTEN was a target of miR-17-5p in the colon cancer cells and their context-specific interactions were responsible for multiple drug-resistance; Chemotherapy was found to increase the expression levels of miR-17-5p which further repressed PTEN levels contributing to the development of chemo-resistance; MiR-17-5p is a predictive factor for chemotherapy response and a prognostic factor for overall survival in CRC which is due to its regulation of PTEN expression;The mature miR-17-5p exerted this function by repressing the expression of PTEN drug resistance;;;;metastasis;;;progression;drug resistance;metastasis;drug resistance;poor survival; pancreatic cancer;glioblastoma;sarcoma;breast cancer;sarcoma;prostate cancer;colorectal cancer;B cell lymphoma;ovarian cancer;colorectal cancer;liver cancer hsa-miR-181c-5p PTEN 0.336826967681288 0.112932307913601 -0.439862628350054 0.00288389874110071 MirTarget;miRNATAP -0.152032634623252 0.00243414632288238 25695913 miR 181c promotes proliferation via suppressing PTEN expression in inflammatory breast cancer; In this study we showed that miR-181c as an oncogene promoted proliferation and it inhibited PTEN protein expression by targeting 3'-UTR of PTEN mRNA in IBC SUM149 cells; Thus targeting miR-181c and restoration of PTEN can be used in conjunction with other therapies to prevent progression of IBC progression breast cancer hsa-miR-181d-5p PTEN 0.65192727601808 0.0143398117228333 -0.439862628350054 0.00288389874110071 MirTarget -0.119382928028224 0.00248265906348588 27006767 Moreover hsa-miR-181d was upregulated to control expression a tumor suppressor gene PTEN to protect the cancer cell from apoptosis endometrial cancer hsa-miR-186-5p PTEN 0.149964130725901 0.434709542998771 -0.439862628350054 0.00288389874110071 mirMAP;miRNATAP -0.152167020094511 0.0067096432586269 NA NA NA hsa-miR-18a-5p PTEN 2.20211504140984 1.78678887916334e-09 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase -0.100407705340982 0.000229468335660971 27291152;24681249 miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis;However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis ;metastasis esophageal cancer;colon cancer hsa-miR-20a-5p PTEN 1.4503375861709 5.7813456424762e-07 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase;miRNATAP -0.125575661981392 0.000343330739702887 26031366 The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction; MiR-20a levels were increased in HCC cell lines and tissues whereas PTEN was inversely correlated with it; PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance drug resistance liver cancer hsa-miR-222-3p PTEN 1.39382731585819 9.98182478024671e-07 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase -0.108079296157776 0.00265168006762418 27746366;23994196;24895988;20618998;26535064;23974492;27699665;27566197 Moreover upregulation of miR-222 expression in MCF-7/S cells is associated with decreased PTEN expression levels and increased phospho-Akt p-Akt expression; Conversely in MCF-7/ADR cells inhibition of miR-222 resulted in increased PTEN expression and decreased p-Akt expression;Western blot results suggested that miR-222 and -29a could regulate the expression of PTEN maybe through which the two miRNAs conferred Adr and Doc resistance in MCF-7 cells; The most importance is that we identify two miRNAs miR-222 and miR-29a involved in drug-resistance at least in part via targeting PTEN;The reduced the expression of PTEN and p27 by miR-222 in HeLa cells and SiHa cells was associated with increased proliferation and migration of CC cells; MiR-222 plays an important role in the tumorigenesis of CC possibly by specifically down-regulating p27Kip1 and PTEN;MicroRNA 221 and microRNA 222 regulate gastric carcinoma cell proliferation and radioresistance by targeting PTEN; PTEN-3'UTR luciferase reporter assay confirmed PTEN as a direct target of miR-221 and miR-222; These results demonstrate that miR-221 and miR-222 regulate radiosensitivity and cell growth and invasion of SGC7901 cells possibly via direct modulation of PTEN expression;MiR-222 and putative target gene expression levels including p27 p57 and PTEN were determined using quantitative reverse transcription polymerase chain reactions and Western blotting; Further we found p57 at protein level but not p27 nor PTEN was regulated by miR-222 in Capan-2 cells;MiR-222 induces cell growth and cell cycle progression via direct targeting of p27 p57 and PTEN in cancer cells;The results showed that inhibition of miR-222 in MCF-7/Adr significantly increased the expressions of PTEN and p27 kip1 and decreased phospho-Akt p-Akt both in mRNA and protein levels p < 0.05 by using quantitative real-time PCR qRT-PCR and western blot; Conversely in MCF-7/S transfected with miR-222 mimics upregulation of miR-222 is associated with decreasing PTEN and p27 kip1 and increasing Akt accompanied by less apoptosis and higher IC50;The expression of tumor suppressor p27 and PTEN miR-222 target genes was significantly increased by PNS treatment ;drug resistance;tumorigenesis;drug resistance;;progression;; breast cancer;breast cancer;cervical and endocervical cancer;gastric cancer;pancreatic cancer;lung cancer;breast cancer;lung cancer hsa-miR-25-3p PTEN 1.00604871526544 0.000421838061147906 -0.439862628350054 0.00288389874110071 miRTarBase;MirTarget;miRNATAP -0.141857747668459 8.68431873420322e-05 NA NA NA hsa-miR-335-3p PTEN 2.5197689063267 2.20084506976733e-09 -0.439862628350054 0.00288389874110071 MirTarget -0.121544500600449 1.68854176574293e-07 NA NA NA hsa-miR-421 PTEN 1.80720854070385 1.59709651202848e-07 -0.439862628350054 0.00288389874110071 miRanda -0.110715787301831 0.000153716455337487 NA NA NA hsa-miR-425-5p PTEN 1.13924952403101 0.000255357546232931 -0.439862628350054 0.00288389874110071 miRNATAP -0.136749196412418 3.2459172510343e-05 25154996 An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation gastric cancer hsa-miR-454-3p PTEN 0.625199142970745 0.0134890997402741 -0.439862628350054 0.00288389874110071 MirTarget;miRNATAP -0.14154447102058 0.000638326410710137 26296312;27261580 MicroRNA 454 functions as an oncogene by regulating PTEN in uveal melanoma; Furthermore we identified PTEN as a direct target of miR-454; Our data revealed that ectopic expression of PTEN restored the effects of miR-454 on cell proliferation and invasion in uveal melanoma cells;MiR 454 promotes the progression of human non small cell lung cancer and directly targets PTEN; At last the potential regulatory function of miR-454 on PTEN expression was confirmed; Further PTEN was confirmed as a direct target of miR-454 by using Luciferase Reporter Assay ;progression melanoma;lung squamous cell cancer hsa-miR-484 PTEN 0.71327898984355 0.00233605130771196 -0.439862628350054 0.00288389874110071 miRNATAP -0.123324186207252 0.00608200697888501 NA NA NA hsa-miR-532-5p PTEN 0.313080805824184 0.252248939356301 -0.439862628350054 0.00288389874110071 PITA;mirMAP;miRNATAP -0.137190125334249 0.00035870173357934 NA NA NA hsa-miR-589-3p PTEN 1.01962606923682 0.00577853347252709 -0.439862628350054 0.00288389874110071 MirTarget;mirMAP -0.108837670957723 9.77792683670741e-05 NA NA NA hsa-miR-590-5p PTEN 1.04171442093217 0.000272475544954865 -0.439862628350054 0.00288389874110071 mirMAP -0.104718214872404 0.00394248736334067 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-7-1-3p PTEN 0.709309110750947 0.0412301737463194 -0.439862628350054 0.00288389874110071 mirMAP -0.123402510670047 3.43125792570084e-05 NA NA NA hsa-miR-92a-3p PTEN 1.2209325870225 6.8757197708107e-06 -0.439862628350054 0.00288389874110071 MirTarget;miRNATAP -0.14277985087479 0.000153445490465264 26432332;25515201;24137349;23546593;23133552;24026406 Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor; Taken together these findings suggested that miR-92a could promote growth metastasis and chemoresistance in NSCLC cells at least partially by targeting PTEN;MiR 92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN Mediated PI3K/AKT Pathway; The expression of miR-92a PTEN and E-cadherin was analyzed by real-time PCR; In addition there was a negative correlation between levels of miR-92a and the PTEN gene p < 0.0001; The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;The expression levels of miR-92a and phosphatase and tensin homologue PTEN were detected by qRT-PCR and western blot; In addition the regulation of PTEN by miR-92a was evaluated by qRT-PCR western blot and luciferase reporter assays; There was an inverse correlation between the levels of miR-92a and PTEN in CRC tissues; The overexpression of miR-92a in CRC cells decreased PTEN expression at the translational level and decreased PTEN-driven luciferase-reporter activity; Our results demonstrated that miR-92a induced EMT and regulated cell growth migration and invasion in the SW480 cells at least partially via suppression of PTEN expression metastasis;drug resistance;metastasis;cell migration;;worse prognosis;; lung squamous cell cancer;colorectal cancer;cervical and endocervical cancer;liver cancer;sarcoma;colorectal cancer hsa-miR-93-5p PTEN 1.7515174434778 1.59386482413883e-08 -0.439862628350054 0.00288389874110071 miRNAWalker2_validate;miRTarBase;miRNATAP -0.149740498224861 3.14876574637166e-06 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-455-5p RCHY1 1.20462751989145 6.74386011867484e-05 -0.491743414198709 0.00367281313532795 miRanda -0.107992716975208 0.00611929803255468 NA NA NA hsa-miR-335-5p RPRM 1.59953351087307 5.56170899867314e-05 -4.16323217505801 3.7272603451261e-07 MirTarget;miRNATAP -0.518490842571238 0.000465997891784612 NA NA NA hsa-miR-338-3p RPRM -0.959276231066179 0.0191473351014091 -4.16323217505801 3.7272603451261e-07 miRanda -0.545585650021564 0.000170685866955241 NA NA NA hsa-miR-375 RPRM -2.94506033785642 0.00570147601654791 -4.16323217505801 3.7272603451261e-07 miRanda -0.184145749641868 0.000913374238377177 NA NA NA hsa-miR-501-3p RPRM 0.731876211669996 0.0270421347018303 -4.16323217505801 3.7272603451261e-07 miRNATAP -0.610232863101497 0.00074752281648755 NA NA NA hsa-miR-100-5p RRM2 -0.909635282943505 0.0262703445792608 3.53384203439172 4.25251266774112e-24 miRNAWalker2_validate -0.25881473456929 0.00013521507892223 NA NA NA hsa-miR-125a-5p RRM2 -0.878090699011564 0.000213630844319685 3.53384203439172 4.25251266774112e-24 miRanda -0.476267940281233 4.71467894340908e-05 NA NA NA hsa-miR-26a-5p RRM2 -0.384110714440647 0.0442498636014044 3.53384203439172 4.25251266774112e-24 miRNAWalker2_validate -0.415746928136287 0.00580388996849328 NA NA NA hsa-miR-30a-5p RRM2 -1.71922855140016 3.20574349285256e-07 3.53384203439172 4.25251266774112e-24 miRNAWalker2_validate -0.408204564004251 2.71083464164273e-07 NA NA NA hsa-miR-30c-5p RRM2 -0.983130268805942 4.60419218706127e-05 3.53384203439172 4.25251266774112e-24 miRNAWalker2_validate -0.402840747336009 0.000469442478820272 NA NA NA hsa-miR-140-5p RRM2B 0.208193069681326 0.303002433893804 0.0645374442626387 0.733817385913298 miRanda -0.344944875398572 1.85713244662e-07 NA NA NA hsa-miR-26a-2-3p RRM2B 0.479058819024867 0.126162666505883 0.0645374442626387 0.733817385913298 mirMAP -0.11713599190997 0.00702148189786648 NA NA NA hsa-miR-28-5p RRM2B -0.238617853755144 0.167318538669818 0.0645374442626387 0.733817385913298 miRanda -0.346555362777845 1.14435913752087e-05 NA NA NA hsa-miR-7-1-3p RRM2B 0.709309110750947 0.0412301737463194 0.0645374442626387 0.733817385913298 mirMAP -0.118835840278733 0.0019934681918241 NA NA NA hsa-miR-129-5p SERPINB5 -2.56914016541176 2.36237896095709e-06 4.78118461455252 1.44058285884666e-10 miRanda -0.399233269993739 7.10562502129018e-05 NA NA NA hsa-miR-186-5p SERPINB5 0.149964130725901 0.434709542998771 4.78118461455252 1.44058285884666e-10 mirMAP -1.01493047764307 0.000647428460628283 NA NA NA hsa-miR-30a-5p SERPINB5 -1.71922855140016 3.20574349285256e-07 4.78118461455252 1.44058285884666e-10 miRNAWalker2_validate -0.859621479659971 4.38374314324764e-08 NA NA NA hsa-miR-338-3p SERPINB5 -0.959276231066179 0.0191473351014091 4.78118461455252 1.44058285884666e-10 miRanda -0.650927174582851 9.85123846950036e-07 NA NA NA hsa-miR-129-5p SERPINE1 -2.56914016541176 2.36237896095709e-06 0.873189054905582 0.11255072331134 miRanda -0.19644165437006 0.00589849447253088 NA NA NA hsa-miR-148a-3p SERPINE1 -1.53721533763578 3.55533747293117e-05 0.873189054905582 0.11255072331134 miRNATAP -0.436559131161047 1.9709933577873e-05 NA NA NA hsa-miR-148a-5p SERPINE1 -0.69329616490858 0.0793012233740369 0.873189054905582 0.11255072331134 miRNATAP -0.527300729477906 4.57253053812486e-08 NA NA NA hsa-miR-2110 SERPINE1 0.759864042760204 0.0723961390557655 0.873189054905582 0.11255072331134 miRNATAP -0.330642930294297 0.000392739120335177 NA NA NA hsa-miR-30b-5p SERPINE1 -0.430720743745757 0.0593564057320791 0.873189054905582 0.11255072331134 MirTarget -0.723431021861993 2.57885394455558e-05 25170877 miR-30b may function as a novel tumor suppressor gene in gastric cancer by targeting PAI-1 and regulating the apoptosis of cancer cells gastric cancer hsa-miR-30c-5p SERPINE1 -0.983130268805942 4.60419218706127e-05 0.873189054905582 0.11255072331134 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.480138224039187 0.00294414713695789 NA NA NA hsa-miR-30d-5p SERPINE1 -0.5492586056147 0.0140125082460125 0.873189054905582 0.11255072331134 miRNATAP -0.997455560362319 6.34120387227326e-09 NA NA NA hsa-miR-378a-5p SERPINE1 -0.557166502179376 0.0344581590006454 0.873189054905582 0.11255072331134 MirTarget -0.612665935436298 3.5388157525321e-05 NA NA NA hsa-miR-769-5p SERPINE1 1.14821899306381 1.27143750879772e-06 0.873189054905582 0.11255072331134 MirTarget;miRNATAP -0.506471309568269 0.0018700765705002 NA NA NA hsa-miR-15a-5p SESN1 1.03783090449383 7.40334108980898e-07 -1.3140373762356 2.23047685422573e-06 MirTarget;miRNATAP -0.258271753370277 0.00699642125003549 NA NA NA hsa-miR-15b-5p SESN1 1.62046407540723 5.42152621571078e-09 -1.3140373762356 2.23047685422573e-06 MirTarget;miRNATAP -0.316374590274581 5.133076647451e-06 NA NA NA hsa-miR-183-5p SESN1 1.65900927871187 0.000522025364980187 -1.3140373762356 2.23047685422573e-06 MirTarget -0.181891346468142 9.660576004955e-06 NA NA NA hsa-miR-21-5p SESN1 1.75445050487988 2.41825549682022e-23 -1.3140373762356 2.23047685422573e-06 miRNAWalker2_validate -0.507081488339274 7.28975441116241e-07 NA NA NA hsa-miR-22-3p SESN1 0.0312103895892015 0.845091185648687 -1.3140373762356 2.23047685422573e-06 MirTarget -0.507837215541602 0.000108912846600655 NA NA NA hsa-miR-24-3p SESN1 1.08578167539644 6.18549359075268e-07 -1.3140373762356 2.23047685422573e-06 miRNAWalker2_validate;MirTarget;miRNATAP -0.440521845510182 9.8819260536667e-07 NA NA NA hsa-miR-199a-5p SESN2 0.371973504507027 0.232661597228763 -0.346797918080422 0.1672553147797 miRNATAP -0.248498514718859 1.16515529360141e-05 NA NA NA hsa-miR-23a-3p SESN2 1.10685926969665 3.16223452471768e-08 -0.346797918080422 0.1672553147797 miRNATAP -0.291250584836407 0.000859684739557093 NA NA NA hsa-miR-106b-5p SESN3 1.70863907461894 2.04074794107142e-08 0.0541364597166463 0.927938931974638 MirTarget -0.470403326758672 0.000469390007036271 NA NA NA hsa-miR-126-5p SESN3 0.41765430251855 0.0753161203651532 0.0541364597166463 0.927938931974638 mirMAP -0.502959451829718 0.0061634771090583 NA NA NA hsa-miR-17-5p SESN3 1.65862087862483 8.45603406215941e-08 0.0541364597166463 0.927938931974638 MirTarget;TargetScan -0.352567852143784 0.00834146431823007 NA NA NA hsa-miR-181b-5p SESN3 1.64216500592669 1.89228845300725e-08 0.0541364597166463 0.927938931974638 MirTarget -0.495585085954948 0.000413497591349389 NA NA NA hsa-miR-186-5p SESN3 0.149964130725901 0.434709542998771 0.0541364597166463 0.927938931974638 MirTarget -1.01605147466484 5.7995677037275e-06 NA NA NA hsa-miR-192-3p SESN3 0.355565421687399 0.74117031340037 0.0541364597166463 0.927938931974638 MirTarget -0.351350833410407 1.2430196546567e-22 NA NA NA hsa-miR-19b-1-5p SESN3 1.38738648038895 1.3113681938066e-06 0.0541364597166463 0.927938931974638 MirTarget -0.556176645463092 0.000113244990746244 NA NA NA hsa-miR-200a-5p SESN3 1.50371809098978 0.00263602434344863 0.0541364597166463 0.927938931974638 mirMAP -0.311927433001086 0.000186151639743479 NA NA NA hsa-miR-200b-3p SESN3 0.974577911407732 0.0595020056914543 0.0541364597166463 0.927938931974638 MirTarget;TargetScan -0.301858914356634 0.000203414526338888 NA NA NA hsa-miR-200b-5p SESN3 0.969871521250413 0.0530500454130183 0.0541364597166463 0.927938931974638 mirMAP -0.429592843895096 7.35718067341207e-07 NA NA NA hsa-miR-20a-5p SESN3 1.4503375861709 5.7813456424762e-07 0.0541364597166463 0.927938931974638 MirTarget -0.422344391691449 0.00318391498471978 NA NA NA hsa-miR-25-3p SESN3 1.00604871526544 0.000421838061147906 0.0541364597166463 0.927938931974638 MirTarget;miRNATAP -0.399335649983989 0.00709909597717202 NA NA NA hsa-miR-26b-3p SESN3 -0.0103961389461977 0.965770899625511 0.0541364597166463 0.927938931974638 MirTarget -0.5916741281848 0.000868567077805417 NA NA NA hsa-miR-29a-5p SESN3 0.585084040404152 0.023014129158495 0.0541364597166463 0.927938931974638 MirTarget;mirMAP -0.776474932294576 1.98406841231264e-06 NA NA NA hsa-miR-29b-1-5p SESN3 0.606560155953522 0.116355599574769 0.0541364597166463 0.927938931974638 mirMAP -0.423907769293654 0.000107532605665762 NA NA NA hsa-miR-30d-3p SESN3 -0.545113326540761 0.0433733076874155 0.0541364597166463 0.927938931974638 mirMAP -0.553115716716083 0.000455976318399216 NA NA NA hsa-miR-32-3p SESN3 0.583525764322974 0.11837203008551 0.0541364597166463 0.927938931974638 mirMAP -0.457524381234328 0.00033234151303272 NA NA NA hsa-miR-32-5p SESN3 0.415247682945382 0.106458022460373 0.0541364597166463 0.927938931974638 MirTarget;miRNATAP -0.45900614640608 0.00602970143328291 NA NA NA hsa-miR-320a SESN3 0.593888711838028 0.011897419390526 0.0541364597166463 0.927938931974638 miRanda;mirMAP -0.481820907378203 0.00809111224233697 NA NA NA hsa-miR-320b SESN3 1.11183660885284 0.000497971211648503 0.0541364597166463 0.927938931974638 miRanda;mirMAP -0.377270791827845 0.00427678604149642 NA NA NA hsa-miR-335-3p SESN3 2.5197689063267 2.20084506976733e-09 0.0541364597166463 0.927938931974638 mirMAP -0.373223051416314 9.85649682924582e-05 NA NA NA hsa-miR-335-5p SESN3 1.59953351087307 5.56170899867314e-05 0.0541364597166463 0.927938931974638 miRNAWalker2_validate -0.493013539850002 1.83002547569408e-06 NA NA NA hsa-miR-338-5p SESN3 -1.19659203838734 0.0100316010080496 0.0541364597166463 0.927938931974638 mirMAP -0.498424711681785 1.53552010167348e-08 NA NA NA hsa-miR-429 SESN3 1.40182602301928 0.00900426780286475 0.0541364597166463 0.927938931974638 MirTarget -0.273182538361756 0.000469682743176229 NA NA NA hsa-miR-454-3p SESN3 0.625199142970745 0.0134890997402741 0.0541364597166463 0.927938931974638 mirMAP -0.439735168719929 0.00943670406578472 NA NA NA hsa-miR-500a-5p SESN3 0.00337014556047865 0.992120109131868 0.0541364597166463 0.927938931974638 mirMAP -0.615341745760634 5.22669483012481e-07 NA NA NA hsa-miR-550a-5p SESN3 2.27360629906903 2.14504977831873e-07 0.0541364597166463 0.927938931974638 MirTarget -0.385522549572509 3.60873255715519e-05 NA NA NA hsa-miR-577 SESN3 0.911619788986754 0.2256062124148 0.0541364597166463 0.927938931974638 mirMAP -0.345236378672303 4.95299326923754e-10 NA NA NA hsa-miR-589-5p SESN3 0.702695092403762 0.00395772685954799 0.0541364597166463 0.927938931974638 MirTarget -0.504642924349721 0.00396817065441531 NA NA NA hsa-miR-616-5p SESN3 0.834052908652744 0.0347835664029363 0.0541364597166463 0.927938931974638 mirMAP -0.295893903799733 0.00691457389614902 NA NA NA hsa-miR-625-3p SESN3 -0.185298294014366 0.626215541224408 0.0541364597166463 0.927938931974638 mirMAP -0.760119146433104 8.2609270766415e-13 NA NA NA hsa-miR-625-5p SESN3 0.537211086526376 0.203801009380384 0.0541364597166463 0.927938931974638 MirTarget -0.706434283339212 1.27562296413213e-13 NA NA NA hsa-miR-7-1-3p SESN3 0.709309110750947 0.0412301737463194 0.0541364597166463 0.927938931974638 MirTarget;mirMAP -0.827173602835019 9.65597963126767e-13 NA NA NA hsa-miR-877-5p SESN3 2.17859965442336 1.03593450648516e-07 0.0541364597166463 0.927938931974638 mirMAP -0.359351430089697 0.000318619082151202 NA NA NA hsa-miR-92b-3p SESN3 1.33518446513417 1.45350885391576e-06 0.0541364597166463 0.927938931974638 MirTarget;miRNATAP -0.396879971725106 0.00849159732678326 NA NA NA hsa-miR-140-5p SIAH1 0.208193069681326 0.303002433893804 0.453374139683266 0.012152958644211 PITA;miRanda;miRNATAP -0.262576242747044 4.49072641426981e-05 NA NA NA hsa-miR-30a-3p SIAH1 -1.73205137750899 3.2425754511091e-07 0.453374139683266 0.012152958644211 miRNATAP -0.142072064221138 0.000100117219419119 NA NA NA hsa-miR-30d-3p SIAH1 -0.545113326540761 0.0433733076874155 0.453374139683266 0.012152958644211 miRNATAP -0.195037825741022 3.82262001268951e-05 NA NA NA hsa-miR-362-3p SIAH1 -0.0349414695893027 0.913780708043777 0.453374139683266 0.012152958644211 miRanda -0.156974052228538 7.99855871102925e-05 NA NA NA hsa-miR-532-5p SIAH1 0.313080805824184 0.252248939356301 0.453374139683266 0.012152958644211 PITA;miRNATAP -0.274436102819604 2.02137129619952e-09 NA NA NA hsa-miR-140-5p STEAP3 0.208193069681326 0.303002433893804 -0.208150968115569 0.469415875455136 PITA;miRanda -0.339086435721582 0.000991451967143119 NA NA NA hsa-let-7a-3p THBS1 0.503048155002889 0.0411145454335299 -1.24863771457813 0.00265778570679874 mirMAP -0.370316910362625 0.00243643875584214 NA NA NA hsa-let-7d-5p THBS1 0.981733854788227 7.26366577706469e-07 -1.24863771457813 0.00265778570679874 miRNAWalker2_validate -0.546987837424227 0.000232224726694981 NA NA NA hsa-miR-151a-3p THBS1 1.00322719901751 7.20903658270795e-06 -1.24863771457813 0.00265778570679874 MirTarget -0.73761432994829 9.23927372162971e-09 NA NA NA hsa-miR-16-1-3p THBS1 1.43227522002564 3.70920454953168e-08 -1.24863771457813 0.00265778570679874 MirTarget -0.475768138699209 1.58834110541284e-05 NA NA NA hsa-miR-17-5p THBS1 1.65862087862483 8.45603406215941e-08 -1.24863771457813 0.00265778570679874 miRNAWalker2_validate -0.477234270724459 1.76065486884735e-07 NA NA NA hsa-miR-182-5p THBS1 0.887369486030646 0.0310640781418789 -1.24863771457813 0.00265778570679874 MirTarget -0.309551358917175 1.40311941430649e-05 24053448 Effects of anti miR 182 on TSP 1 expression in human colon cancer cells: there is a sense in antisense?; We investigate on the molecular mechanism by which miR-182 could regulate thrombospondin-1 TSP-1 expression a protein downregulated in CRC and inversely correlated with tumor vascularity and metastasis; miR-182 over-expressed in colorectal cancer CRC has like predictive target thrombospondin-1 TSP-1 a protein inversely correlated with tumor vascularity and metastasis that results downregulated in different types of cancer including CRC; We found that TSP-1 increased after transfection with anti-miR-182 and we showed that miR-182 targets TSP-1 3'UTR-mRNA in both cells; Our data suggest that miR-182 targets the anti-angiogenic factor TSP-1 and that anti-miR-182 determines an upregulation of TSP-1 expression in colon cancer cells metastasis colon cancer hsa-miR-186-5p THBS1 0.149964130725901 0.434709542998771 -1.24863771457813 0.00265778570679874 mirMAP -0.520998790228557 0.00106972433199894 NA NA NA hsa-miR-18a-5p THBS1 2.20211504140984 1.78678887916334e-09 -1.24863771457813 0.00265778570679874 MirTarget -0.397796098250484 1.86835263945503e-07 NA NA NA hsa-miR-19a-3p THBS1 1.26669687448473 0.000105972838514412 -1.24863771457813 0.00265778570679874 MirTarget;mirMAP -0.373099495159569 2.8124340301922e-05 NA NA NA hsa-miR-19b-3p THBS1 0.759818275708988 0.00652779573186786 -1.24863771457813 0.00265778570679874 MirTarget;mirMAP -0.475934731509183 6.15248588571142e-06 NA NA NA hsa-miR-200a-5p THBS1 1.50371809098978 0.00263602434344863 -1.24863771457813 0.00265778570679874 MirTarget -0.391138424820444 2.988456435559e-12 NA NA NA hsa-miR-200b-5p THBS1 0.969871521250413 0.0530500454130183 -1.24863771457813 0.00265778570679874 MirTarget -0.428970652872064 2.73273845947343e-13 NA NA NA hsa-miR-20a-5p THBS1 1.4503375861709 5.7813456424762e-07 -1.24863771457813 0.00265778570679874 miRNAWalker2_validate -0.49794382461585 4.0938227581297e-07 NA NA NA hsa-miR-3065-5p THBS1 -0.241623656423063 0.633116553078159 -1.24863771457813 0.00265778570679874 MirTarget -0.249036989118658 3.04281341597343e-05 NA NA NA hsa-miR-32-5p THBS1 0.415247682945382 0.106458022460373 -1.24863771457813 0.00265778570679874 mirMAP -0.5555340737988 1.3387339745154e-06 NA NA NA hsa-miR-335-3p THBS1 2.5197689063267 2.20084506976733e-09 -1.24863771457813 0.00265778570679874 mirMAP -0.401833811450832 7.76964738276262e-10 NA NA NA hsa-miR-33a-3p THBS1 0.347011402017138 0.321709379745392 -1.24863771457813 0.00265778570679874 MirTarget;mirMAP -0.531341072213548 9.05114561069452e-10 NA NA NA hsa-miR-375 THBS1 -2.94506033785642 0.00570147601654791 -1.24863771457813 0.00265778570679874 miRanda -0.121276767953717 8.45936054053377e-06 NA NA NA hsa-miR-576-5p THBS1 0.625714137488957 0.0417100301848969 -1.24863771457813 0.00265778570679874 mirMAP -0.41902887625134 1.27872122980434e-05 NA NA NA hsa-miR-577 THBS1 0.911619788986754 0.2256062124148 -1.24863771457813 0.00265778570679874 mirMAP -0.235350281618529 1.68539263735026e-09 NA NA NA hsa-miR-590-3p THBS1 1.11654983899947 0.000160782154924864 -1.24863771457813 0.00265778570679874 miRanda -0.453209723492059 3.97290709629156e-06 NA NA NA hsa-miR-590-5p THBS1 1.04171442093217 0.000272475544954865 -1.24863771457813 0.00265778570679874 miRanda;mirMAP -0.477091842103574 2.72141337877697e-06 NA NA NA hsa-miR-616-5p THBS1 0.834052908652744 0.0347835664029363 -1.24863771457813 0.00265778570679874 mirMAP -0.231533516554552 0.00253501598736982 NA NA NA hsa-miR-629-3p THBS1 1.40326397107309 9.7803063526732e-05 -1.24863771457813 0.00265778570679874 MirTarget -0.437479666325319 3.50844628225438e-08 NA NA NA hsa-miR-7-1-3p THBS1 0.709309110750947 0.0412301737463194 -1.24863771457813 0.00265778570679874 MirTarget;mirMAP -0.367898102747668 1.39963947465173e-05 NA NA NA hsa-miR-92a-3p THBS1 1.2209325870225 6.8757197708107e-06 -1.24863771457813 0.00265778570679874 miRNAWalker2_validate -0.651028463510854 4.08823366684469e-10 NA NA NA hsa-miR-940 THBS1 1.64406174271686 0.000690854900107004 -1.24863771457813 0.00265778570679874 MirTarget -0.24008111432213 0.000798882289984547 NA NA NA hsa-miR-10a-5p TP73 -0.470861534765518 0.148803248989189 3.13334835059042 2.43758243274273e-06 mirMAP -0.7618169766012 1.80321688089474e-07 NA NA NA hsa-miR-192-5p TP73 0.0809146624034405 0.941057093528709 3.13334835059042 2.43758243274273e-06 mirMAP -0.339324490217586 1.32283206800023e-16 NA NA NA hsa-miR-194-3p TP73 0.259420201261466 0.821811295418686 3.13334835059042 2.43758243274273e-06 mirMAP -0.307721078134838 1.0865169460496e-14 NA NA NA hsa-miR-361-3p TP73 -0.126462931388735 0.566052688733137 3.13334835059042 2.43758243274273e-06 MirTarget -0.698422152270084 0.00186296102029691 NA NA NA hsa-miR-7-5p TP73 1.63575693499363 0.0124424214624534 3.13334835059042 2.43758243274273e-06 mirMAP -0.266650599567726 0.000512113251627234 NA NA NA hsa-let-7f-1-3p ZMAT3 1.28704406797397 1.17954701106157e-06 -0.258461699304846 0.22453443414405 mirMAP -0.179891035015217 0.00123224114011596 NA NA NA hsa-miR-106b-5p ZMAT3 1.70863907461894 2.04074794107142e-08 -0.258461699304846 0.22453443414405 mirMAP -0.187949982938655 7.36701633261984e-05 NA NA NA hsa-miR-126-5p ZMAT3 0.41765430251855 0.0753161203651532 -0.258461699304846 0.22453443414405 mirMAP -0.23896304604092 0.000211885497786317 NA NA NA hsa-miR-148b-5p ZMAT3 1.04409494910362 0.00195860954157389 -0.258461699304846 0.22453443414405 MirTarget -0.153718090725325 0.000498098191036341 NA NA NA hsa-miR-150-5p ZMAT3 0.147461377429675 0.753719621305461 -0.258461699304846 0.22453443414405 mirMAP -0.100587321544302 0.00166564086195537 NA NA NA hsa-miR-15a-5p ZMAT3 1.03783090449383 7.40334108980898e-07 -0.258461699304846 0.22453443414405 MirTarget -0.192663640187637 0.00683774831259178 NA NA NA hsa-miR-16-5p ZMAT3 1.00515772948091 5.33912942007619e-06 -0.258461699304846 0.22453443414405 MirTarget -0.183575753136206 0.00675302905494672 NA NA NA hsa-miR-17-5p ZMAT3 1.65862087862483 8.45603406215941e-08 -0.258461699304846 0.22453443414405 mirMAP -0.156953970395732 0.000855771930635305 NA NA NA hsa-miR-186-5p ZMAT3 0.149964130725901 0.434709542998771 -0.258461699304846 0.22453443414405 MirTarget -0.256856708521433 0.00140405947961202 NA NA NA hsa-miR-18a-3p ZMAT3 2.35188582849667 1.39401350286321e-08 -0.258461699304846 0.22453443414405 MirTarget -0.156087254161653 6.07336611689587e-06 NA NA NA hsa-miR-193a-3p ZMAT3 0.214911823818578 0.399323056245552 -0.258461699304846 0.22453443414405 MirTarget -0.190501508227706 0.00141046123166677 NA NA NA hsa-miR-19a-3p ZMAT3 1.26669687448473 0.000105972838514412 -0.258461699304846 0.22453443414405 MirTarget -0.168247781382943 0.000195537407415323 NA NA NA hsa-miR-19b-3p ZMAT3 0.759818275708988 0.00652779573186786 -0.258461699304846 0.22453443414405 MirTarget -0.179533619098985 0.000839830281217676 NA NA NA hsa-miR-20a-5p ZMAT3 1.4503375861709 5.7813456424762e-07 -0.258461699304846 0.22453443414405 mirMAP -0.15722512967492 0.0019069027205805 NA NA NA hsa-miR-223-5p ZMAT3 1.22031379538176 0.0021883283309711 -0.258461699304846 0.22453443414405 mirMAP -0.14424477638608 0.00011189708811338 NA NA NA hsa-miR-29b-1-5p ZMAT3 0.606560155953522 0.116355599574769 -0.258461699304846 0.22453443414405 mirMAP -0.139759338364787 0.000322773601747892 NA NA NA hsa-miR-320a ZMAT3 0.593888711838028 0.011897419390526 -0.258461699304846 0.22453443414405 miRanda;mirMAP -0.184057211260843 0.00422104722611791 NA NA NA hsa-miR-320b ZMAT3 1.11183660885284 0.000497971211648503 -0.258461699304846 0.22453443414405 miRanda;mirMAP -0.195532132576096 2.29274471256344e-05 NA NA NA hsa-miR-320c ZMAT3 0.455072851945474 0.240613785444485 -0.258461699304846 0.22453443414405 miRanda;mirMAP -0.122046055126061 0.00684673325686186 NA NA NA hsa-miR-330-3p ZMAT3 0.798460296022179 0.00746538156533435 -0.258461699304846 0.22453443414405 MirTarget;miRNATAP -0.133980974231778 0.00798762650872853 NA NA NA hsa-miR-421 ZMAT3 1.80720854070385 1.59709651202848e-07 -0.258461699304846 0.22453443414405 mirMAP;miRNATAP -0.117087061109041 0.00578804162039063 NA NA NA hsa-miR-425-5p ZMAT3 1.13924952403101 0.000255357546232931 -0.258461699304846 0.22453443414405 MirTarget -0.202072465191075 1.90660075147286e-05 NA NA NA hsa-miR-454-3p ZMAT3 0.625199142970745 0.0134890997402741 -0.258461699304846 0.22453443414405 MirTarget -0.184094872090982 0.00207145198427836 NA NA NA hsa-miR-501-5p ZMAT3 0.266919328110625 0.454779001968586 -0.258461699304846 0.22453443414405 MirTarget;mirMAP -0.16933736690316 5.31357077813104e-05 NA NA NA hsa-miR-550a-3p ZMAT3 2.05849884025997 7.99642185793604e-07 -0.258461699304846 0.22453443414405 mirMAP -0.145762950924142 0.000154534599538753 NA NA NA hsa-miR-590-3p ZMAT3 1.11654983899947 0.000160782154924864 -0.258461699304846 0.22453443414405 mirMAP;miRNATAP -0.135477108666355 0.00728946538659711 NA NA NA hsa-miR-590-5p ZMAT3 1.04171442093217 0.000272475544954865 -0.258461699304846 0.22453443414405 miRanda -0.206633390809993 6.47941586243161e-05 NA NA NA hsa-miR-660-5p ZMAT3 -0.0206552819434673 0.938868734063643 -0.258461699304846 0.22453443414405 mirMAP -0.173139549653072 0.00217915336364514 NA NA NA hsa-miR-7-1-3p ZMAT3 0.709309110750947 0.0412301737463194 -0.258461699304846 0.22453443414405 mirMAP -0.140284409050761 0.00116533560878446 NA NA NA hsa-miR-93-5p ZMAT3 1.7515174434778 1.59386482413883e-08 -0.258461699304846 0.22453443414405 mirMAP -0.137519690141059 0.00342480451649575 NA NA NA hsa-miR-942-5p ZMAT3 1.90585138008274 3.11903311044863e-09 -0.258461699304846 0.22453443414405 MirTarget -0.167610926252702 0.000179602321482695 NA NA NA