miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-100-5p ANAPC11 -0.783576782126614 0.000219958772262677 0.58422633722428 3.95882494846378e-06 miRNAWalker2_validate -0.117758947046495 4.98798222705985e-05 NA NA NA hsa-miR-542-3p ANAPC2 -1.31351015149842 3.64642423288576e-22 0.159952010391626 0.0427425043602504 miRanda -0.130060146590296 1.01942138337224e-06 NA NA NA hsa-miR-30a-5p ANAPC5 -0.626257932887855 0.000108198196973765 0.240399804654223 3.84016801569404e-05 miRNAWalker2_validate -0.10853127062001 2.40299220202072e-10 NA NA NA hsa-miR-139-5p ANAPC7 -2.11299531796557 8.16407076415038e-28 0.839259014209164 3.12717900375048e-28 miRanda -0.201479395472035 1.48359984931874e-29 NA NA NA hsa-miR-30c-2-3p ANAPC7 -1.39811819128587 3.66376924527665e-15 0.839259014209164 3.12717900375048e-28 MirTarget -0.15878641008093 3.03831888957767e-14 NA NA NA hsa-miR-542-3p ANAPC7 -1.31351015149842 3.64642423288576e-22 0.839259014209164 3.12717900375048e-28 miRanda -0.18725211263656 4.99668257692473e-12 NA NA NA hsa-miR-30d-3p ATM -0.120562286038759 0.329549534782125 -0.236058797850924 0.0173776926910908 mirMAP -0.138156674894502 0.00042794166032829 24345332 miR-30d has been observed to be significantly down-regulated in human anaplastic thyroid carcinoma ATC and is believed to be an important event in thyroid cell transformation; In this study we found that miR-30d has a critical role in modulating sensitivity of ATC cells to cisplatin a commonly used chemotherapeutic drug for treatment of this neoplasm; Using a mimic of miR-30d we demonstrated that miR-30d could negatively regulate the expression of beclin 1 a key autophagy gene leading to suppression of the cisplatin-activated autophagic response that protects ATC cells from apoptosis; We further showed that inhibition of the beclin 1-mediated autophagy by the miR-30d mimic sensitized ATC cells to cisplatin both in vitro cell culture and in vivo animal xenograft model; These results suggest that dysregulation of miR-30d in ATC cells is responsible for the insensitivity to cisplatin by promoting autophagic survival; Thus miR-30d may be exploited as a potential target for therapeutic intervention in the treatment of ATC drug resistance;poor survival thyroid cancer hsa-miR-339-5p ATM 0.280589919878422 0.0355660282647215 -0.236058797850924 0.0173776926910908 miRanda -0.1044932927788 0.00399236512159519 NA NA NA hsa-miR-455-5p ATM -0.270546034117157 0.0581320703577731 -0.236058797850924 0.0173776926910908 miRanda -0.118715671502599 0.000450782297458542 NA NA NA hsa-miR-139-5p BUB1 -2.11299531796557 8.16407076415038e-28 4.04762666744344 9.60341711300894e-48 miRanda -0.981222586322315 6.07995380621313e-51 NA NA NA hsa-miR-199a-5p BUB1 -1.98848462405923 2.0421622713753e-11 4.04762666744344 9.60341711300894e-48 miRanda -0.291631300567174 3.71128271880203e-09 NA NA NA hsa-miR-542-3p BUB1 -1.31351015149842 3.64642423288576e-22 4.04762666744344 9.60341711300894e-48 miRanda -0.668988733252235 2.14129391171968e-10 NA NA NA hsa-miR-215-5p BUB1B -0.981544773246509 3.14452272477156e-05 3.85682830100864 2.02974032557381e-43 miRNAWalker2_validate -0.164346842914509 0.0099257038268172 NA NA NA hsa-miR-22-3p BUB1B -0.628561621952407 1.33755558015523e-09 3.85682830100864 2.02974032557381e-43 miRNAWalker2_validate -1.59565755962297 8.34276186949843e-33 NA NA NA hsa-miR-486-5p BUB1B -1.77766890600022 2.9071717929922e-14 3.85682830100864 2.02974032557381e-43 miRanda -0.415413021317956 1.24965935132642e-11 NA NA NA hsa-miR-139-5p BUB3 -2.11299531796557 8.16407076415038e-28 0.484122281215701 1.04333829038208e-13 miRanda -0.120530889452753 2.21957104706434e-15 NA NA NA hsa-let-7a-3p CCNA2 -0.568717557554068 1.00947202557819e-07 3.36794922367745 6.53886370047174e-43 MirTarget -0.508034164420555 3.65278492221913e-05 NA NA NA hsa-let-7b-3p CCNA2 -1.21979213547786 5.84575581584603e-17 3.36794922367745 6.53886370047174e-43 MirTarget -0.732605840868638 4.54728817669008e-18 NA NA NA hsa-let-7b-5p CCNA2 -0.959013717270803 5.95127281258643e-11 3.36794922367745 6.53886370047174e-43 miRNAWalker2_validate;miRTarBase -0.524638048172729 1.97837367658696e-09 NA NA NA hsa-let-7f-1-3p CCNA2 -0.704017923073338 7.84883628944999e-07 3.36794922367745 6.53886370047174e-43 MirTarget -0.411800166097723 9.61755775465089e-06 NA NA NA hsa-miR-130a-3p CCNA2 -1.53229181736223 2.91460363624872e-19 3.36794922367745 6.53886370047174e-43 miRNATAP -0.393381177440701 9.03577520347484e-08 NA NA NA hsa-miR-199a-5p CCNA2 -1.98848462405923 2.0421622713753e-11 3.36794922367745 6.53886370047174e-43 miRanda -0.274665722447149 1.39754779993059e-10 NA NA NA hsa-miR-22-3p CCNA2 -0.628561621952407 1.33755558015523e-09 3.36794922367745 6.53886370047174e-43 MirTarget -1.21746436700899 3.56641376559019e-24 25596928 The sequence of miR-22 which is conserved in mice rats humans and other mammalians aligns with the sequence of 3'-UTR of CCNA2; Chenodeoxycholic acid treatment and miR-22 mimics reduced CCNA2 protein and increased the number of G0/G1 Huh7 and HCT116 cells; In humans the expression levels of miR-22 and CCNA2 are inversely correlated in liver and colon cancers liver cancer hsa-miR-27b-3p CCNA2 -0.823491817819754 9.20935302746823e-14 3.36794922367745 6.53886370047174e-43 miRNATAP -0.54120082992627 3.46716161944693e-06 NA NA NA hsa-miR-29a-3p CCNA2 -0.856762511178136 4.65330336378459e-12 3.36794922367745 6.53886370047174e-43 MirTarget -0.878689289923181 3.31167697738131e-18 NA NA NA hsa-miR-29b-1-5p CCNA2 -0.538946836809295 0.00102913307251186 3.36794922367745 6.53886370047174e-43 MirTarget -0.331466426463458 3.78367154695011e-05 NA NA NA hsa-miR-29b-3p CCNA2 -0.348885528720517 0.0121441341291285 3.36794922367745 6.53886370047174e-43 MirTarget -0.589199607558879 3.67803340321416e-10 NA NA NA hsa-miR-29c-3p CCNA2 -1.4367517608807 1.61767774905781e-21 3.36794922367745 6.53886370047174e-43 MirTarget -0.922743011617596 1.63915073620724e-32 NA NA NA hsa-miR-486-5p CCNA2 -1.77766890600022 2.9071717929922e-14 3.36794922367745 6.53886370047174e-43 miRanda -0.275730733902838 4.10950790707083e-07 NA NA NA hsa-let-7b-5p CCNB1 -0.959013717270803 5.95127281258643e-11 3.15540953438863 5.73583669464518e-46 miRNAWalker2_validate -0.536702047843919 1.21768540248182e-11 NA NA NA hsa-miR-139-5p CCNB1 -2.11299531796557 8.16407076415038e-28 3.15540953438863 5.73583669464518e-46 miRanda -0.800903870286373 3.33153076219979e-55 NA NA NA hsa-let-7a-5p CCNB2 -0.328069937201898 0.000464344093744051 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.449095168142698 0.00713886337518027 NA NA NA hsa-let-7b-5p CCNB2 -0.959013717270803 5.95127281258643e-11 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.589177166241791 8.39990492744342e-09 NA NA NA hsa-let-7c-5p CCNB2 -1.70744661345097 2.87934212005942e-20 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.85447689630769 1.64053136609545e-30 NA NA NA hsa-miR-23b-3p CCNB2 -0.530561587109023 5.43807206823652e-07 4.23615330954301 1.17296327500891e-51 miRNAWalker2_validate -0.641555640172717 9.69421048739327e-06 NA NA NA hsa-miR-339-5p CCNB3 0.280589919878422 0.0355660282647215 0.193558872127947 0.274836388284752 miRanda -0.205669923205531 0.00149302963795871 NA NA NA hsa-miR-106a-5p CCND1 -0.464229535269236 0.009724944358047 -0.901747452525989 1.22363850855738e-05 MirTarget;miRNATAP -0.262282596122017 2.76721397734651e-06 NA NA NA hsa-miR-106b-5p CCND1 0.648901865047099 8.24870862047885e-09 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.430796355484411 1.07010119372681e-06 NA NA NA hsa-miR-1266-5p CCND1 1.6344248374643 4.09278929749906e-13 -0.901747452525989 1.22363850855738e-05 MirTarget -0.234489057674296 5.64471200142146e-08 NA NA NA hsa-miR-15b-5p CCND1 0.230566941413564 0.0824765001857479 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.544455359613115 3.17021042565567e-13 NA NA NA hsa-miR-16-5p CCND1 -0.398577235179603 0.000101598495180191 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.309506657035983 0.00178218906910022 23991964;22922827;18483394 At the molecular level our results further revealed that cyclin D1 expression was negatively regulated by miR-16;CCND1 has been found to be a target of miR-15a and miR-16-1 through analysis of complementary sequences between microRNAs and CCND1 mRNA; Moreover the transcription of CCND1 is suppressed by miR-15a and miR-16-1 via direct binding to the CCND1 3'-untranslated region 3'-UTR;Truncation in CCND1 mRNA alters miR 16 1 regulation in mantle cell lymphoma; Furthermore we demonstrated that this truncation alters miR-16-1 binding sites and through the use of reporter constructs we were able to show that miR-16-1 regulates CCND1 mRNA expression; This study introduces the role of miR-16-1 in the regulation of CCND1 in MCL ;; bladder cancer;sarcoma;lymphoma hsa-miR-17-5p CCND1 0.698861147045461 2.43854106455398e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.343463418912801 9.97512783987264e-09 26431674 Bioinformatics Prediction and In Vitro Analysis Revealed That miR 17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells; In this study using bioinformatic analyses miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score; After lentiviral transduction of miR-17 to the target cells gene expression analysis showed decreased expression of CCND1 gene breast cancer hsa-miR-186-5p CCND1 -0.060103391373687 0.535288717081205 -0.901747452525989 1.22363850855738e-05 mirMAP -0.315936974513477 0.00286438511586306 NA NA NA hsa-miR-19a-3p CCND1 1.01712596153672 2.57753201450625e-07 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.283475432466515 1.38758823633513e-08 25985117 Moreover miR-19a might play inhibitory roles in HCC malignancy via regulating Cyclin D1 expression liver cancer hsa-miR-19b-1-5p CCND1 -0.277804782598538 0.0783067794722916 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase -0.307712037252966 1.4364183273616e-06 NA NA NA hsa-miR-19b-3p CCND1 0.601279551368824 0.00016685097085251 -0.901747452525989 1.22363850855738e-05 miRNATAP -0.340975400639976 4.6454998539434e-08 NA NA NA hsa-miR-20a-5p CCND1 0.854326758727003 5.04256669663285e-07 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.332506713675419 1.04479750525145e-08 NA NA NA hsa-miR-20b-5p CCND1 0.457668023910379 0.0285865211936156 -0.901747452525989 1.22363850855738e-05 MirTarget;miRNATAP -0.230429652941375 1.603682834845e-06 NA NA NA hsa-miR-340-5p CCND1 -0.00477614531381576 0.968502722773791 -0.901747452525989 1.22363850855738e-05 mirMAP -0.322810275002772 0.000125999869952101 NA NA NA hsa-miR-425-5p CCND1 0.585974239943864 2.23063410931402e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.385144136522737 9.38557217850495e-08 NA NA NA hsa-miR-503-5p CCND1 0.191949282338049 0.268420664548092 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget -0.155187994375291 0.00815133470022587 26047605;23731275 MiR 503 inhibited cell proliferation of human breast cancer cells by suppressing CCND1 expression; Overexpression of miR-503 in breast cancer cell lines reduced cell proliferation through inducing G0/G1 cell cycle arrest by targeting CCND1;MicroRNA 503 suppresses proliferation and cell cycle progression of endometrioid endometrial cancer by negatively regulating cyclin D1; CCND1 has a binding sequence of miR-503 within its 3' untranslated region and was confirmed to be a direct target of miR-503 by the fluorescent reporter assays; Increasing the miR-503 level in EEC cells suppressed cell viability colon formation activity and cell-cycle progression and the inhibited oncogenic phenotypes induced by miR-503 were alleviated by ectopic expression of CCND1 without the untranslated region sequence; Collectively this study suggested that miR-503 plays a tumor-suppressor role by targeting CCND1; Abnormal suppression of miR-503 leads to an increase in the CCND1 level which may promote carcinogenesis and progression of EEC ;progression;tumorigenesis breast cancer;endometrial cancer hsa-miR-589-3p CCND1 1.16846704028095 5.88525574424201e-11 -0.901747452525989 1.22363850855738e-05 MirTarget -0.183119037529565 0.00124255651400669 NA NA NA hsa-miR-616-5p CCND1 0.150569904638223 0.402836556304524 -0.901747452525989 1.22363850855738e-05 mirMAP -0.256039244652268 5.24430962347992e-06 NA NA NA hsa-miR-7-1-3p CCND1 -0.569105143287799 1.85297665041424e-05 -0.901747452525989 1.22363850855738e-05 mirMAP -0.260985322289835 0.000573950021646959 NA NA NA hsa-miR-9-5p CCND1 1.26407994492898 8.53156156439881e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.136746717035529 1.04306605002938e-05 NA NA NA hsa-miR-92a-3p CCND1 0.212391012518195 0.134292268089997 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.407898716180535 8.06773614790968e-09 NA NA NA hsa-miR-93-5p CCND1 1.39518349670953 2.84167728726712e-25 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.335918242999821 2.01286203755089e-06 NA NA NA hsa-miR-942-5p CCND1 0.346451845105263 0.0283255399972342 -0.901747452525989 1.22363850855738e-05 MirTarget -0.245807225430864 0.000123103109119178 NA NA NA hsa-miR-130b-5p CCND2 0.170599813152408 0.337611586432771 0.356451160382274 0.0365641518216489 mirMAP -0.174593683703476 0.000182311423622 NA NA NA hsa-miR-20a-5p CCND2 0.854326758727003 5.04256669663285e-07 0.356451160382274 0.0365641518216489 miRNAWalker2_validate;miRTarBase;miRNATAP -0.156120931730772 0.0012098497067071 NA NA NA hsa-miR-28-5p CCND2 -0.430964830599964 2.67367257252925e-06 0.356451160382274 0.0365641518216489 miRanda -0.482733599739849 7.0104175669049e-08 NA NA NA hsa-miR-33a-3p CCND2 -0.683025859637737 1.01106097756419e-05 0.356451160382274 0.0365641518216489 MirTarget -0.263538375258973 6.17940317063527e-07 NA NA NA hsa-miR-3607-3p CCND2 -2.15518588554493 1.03390728226728e-23 0.356451160382274 0.0365641518216489 mirMAP -0.12362922199284 0.000700039964538526 NA NA NA hsa-miR-378a-3p CCND2 -1.1920055906972 3.23538871733634e-10 0.356451160382274 0.0365641518216489 miRNAWalker2_validate -0.183441253857915 1.70039721501862e-05 NA NA NA hsa-miR-548v CCND2 -0.27258775900243 0.176259917595269 0.356451160382274 0.0365641518216489 MirTarget -0.149460521918892 0.000339780804187451 NA NA NA hsa-miR-616-5p CCND2 0.150569904638223 0.402836556304524 0.356451160382274 0.0365641518216489 mirMAP -0.315443515897926 3.35063962863299e-12 NA NA NA hsa-miR-618 CCND2 0.13539895350352 0.517145913114103 0.356451160382274 0.0365641518216489 mirMAP -0.225947558771367 2.79586020953898e-08 NA NA NA hsa-miR-27b-3p CCND3 -0.823491817819754 9.20935302746823e-14 0.0767102129984094 0.478427267753328 miRNAWalker2_validate -0.236832359235505 2.50262257150465e-07 NA NA NA hsa-miR-320a CCND3 0.325285936317332 0.0221415065838824 0.0767102129984094 0.478427267753328 miRanda -0.118523664136384 0.00134906533817398 NA NA NA hsa-miR-125b-5p CCNE1 -1.35767721102906 1.16564617395164e-18 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate -0.798490415823305 1.91419546517386e-20 NA NA NA hsa-miR-192-5p CCNE1 -0.50188499512787 0.00344713341196035 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate -0.353990461529605 2.35568212305901e-05 NA NA NA hsa-miR-195-5p CCNE1 -1.85907534356434 1.47434041676402e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;MirTarget;miRNATAP -0.296870668934945 3.50489012043558e-05 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.963792031426845 1.83360999387345e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;miRNATAP -0.598523781447039 1.7327978808332e-05 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-26b-5p CCNE1 -1.10804856399744 4.14176110314282e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;miRNATAP -0.886251741849761 3.83249719633593e-14 NA NA NA hsa-miR-424-5p CCNE1 -2.62653257498508 2.38997974737991e-50 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.365785229181321 3.5890870773903e-07 NA NA NA hsa-miR-497-5p CCNE1 -1.41371173021948 7.09278946477743e-17 3.04640353472202 4.5986250804531e-28 MirTarget;miRNATAP -0.265241585556134 0.00124914232960422 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-let-7b-3p CCNE2 -1.21979213547786 5.84575581584603e-17 2.01834184083791 5.42527234853282e-21 mirMAP -0.268569886454063 0.000212358107274223 NA NA NA hsa-miR-126-3p CCNE2 -0.652349350198172 9.37876280344772e-09 2.01834184083791 5.42527234853282e-21 miRNAWalker2_validate -0.385200741422764 5.31716743581417e-05 NA NA NA hsa-miR-26a-5p CCNE2 -0.963792031426845 1.83360999387345e-22 2.01834184083791 5.42527234853282e-21 miRNAWalker2_validate;miRTarBase;miRNATAP -0.488121351666366 3.8244940657208e-06 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-26b-5p CCNE2 -1.10804856399744 4.14176110314282e-22 2.01834184083791 5.42527234853282e-21 miRNATAP -0.579195472860141 1.12131120131545e-10 NA NA NA hsa-miR-30a-5p CCNE2 -0.626257932887855 0.000108198196973765 2.01834184083791 5.42527234853282e-21 miRNATAP -0.356376866932366 9.6729026873686e-08 NA NA NA hsa-miR-1301-3p CCNH 1.12156714169769 4.22404125385203e-10 -0.436712312869814 1.58751807248243e-07 miRNAWalker2_validate -0.147874673372735 1.38978485989833e-11 NA NA NA hsa-miR-23a-3p CCNH -0.178875974460574 0.135978273540604 -0.436712312869814 1.58751807248243e-07 MirTarget -0.131912078959356 0.000118569572071824 NA NA NA hsa-miR-103a-2-5p CDC14B 1.17085533927423 5.31418969120877e-09 -1.18862488716786 6.19212759828214e-11 MirTarget -0.258689850106033 1.93281821468708e-08 NA NA NA hsa-miR-141-3p CDC14B -0.354316381352484 0.256996894288799 -1.18862488716786 6.19212759828214e-11 mirMAP -0.196426786345194 4.76704735431923e-12 NA NA NA hsa-miR-148b-5p CDC14B 0.295777213389605 0.0255749859535438 -1.18862488716786 6.19212759828214e-11 mirMAP -0.466498442292863 3.67858323120435e-12 NA NA NA hsa-miR-15a-5p CDC14B 0.349521211668244 0.000770735998804736 -1.18862488716786 6.19212759828214e-11 miRNAWalker2_validate;MirTarget -0.264133561370897 0.00255856972749859 NA NA NA hsa-miR-15b-5p CDC14B 0.230566941413564 0.0824765001857479 -1.18862488716786 6.19212759828214e-11 MirTarget -0.295544766632917 1.44522305165738e-05 NA NA NA hsa-miR-17-3p CDC14B 0.410352776142368 0.00422200500748636 -1.18862488716786 6.19212759828214e-11 MirTarget -0.411816770017806 2.8300203911774e-11 NA NA NA hsa-miR-183-5p CDC14B 2.33444328833177 1.52188109365421e-10 -1.18862488716786 6.19212759828214e-11 MirTarget -0.209168889921202 2.23918514009581e-19 NA NA NA hsa-miR-185-5p CDC14B 0.480006257154717 6.35758891340152e-07 -1.18862488716786 6.19212759828214e-11 MirTarget -0.4417635875514 2.08417483328579e-06 NA NA NA hsa-miR-19b-1-5p CDC14B -0.277804782598538 0.0783067794722916 -1.18862488716786 6.19212759828214e-11 mirMAP -0.195003497665162 0.000689359847299325 NA NA NA hsa-miR-200a-3p CDC14B -1.49949292595657 3.18410995256133e-05 -1.18862488716786 6.19212759828214e-11 mirMAP -0.145816275279645 2.99145113535698e-09 NA NA NA hsa-miR-21-3p CDC14B -0.484219378716006 0.00299941098417659 -1.18862488716786 6.19212759828214e-11 mirMAP -0.297956469233426 5.31433385530136e-08 NA NA NA hsa-miR-27a-3p CDC14B -0.370807380415112 0.00875721956202713 -1.18862488716786 6.19212759828214e-11 miRNATAP -0.276640822420895 1.43193730675328e-05 NA NA NA hsa-miR-320a CDC14B 0.325285936317332 0.0221415065838824 -1.18862488716786 6.19212759828214e-11 mirMAP -0.202962844431118 0.0014726065136568 NA NA NA hsa-miR-320b CDC14B 0.0919652022571331 0.607981643616945 -1.18862488716786 6.19212759828214e-11 mirMAP -0.206013690662702 4.86844931867914e-05 NA NA NA hsa-miR-338-5p CDC14B -0.221898432884726 0.252390988727086 -1.18862488716786 6.19212759828214e-11 PITA -0.27425052279737 3.23035807809276e-09 NA NA NA hsa-miR-429 CDC14B -1.40262535827285 6.93982219171912e-05 -1.18862488716786 6.19212759828214e-11 PITA;miRanda;miRNATAP -0.146414069788732 8.47600515344413e-09 NA NA NA hsa-miR-501-3p CDC14B 0.999540908940094 2.77358485581974e-11 -1.18862488716786 6.19212759828214e-11 PITA -0.47280054496801 1.09255307726433e-16 NA NA NA hsa-miR-502-3p CDC14B 0.659598854007546 3.01316453996423e-08 -1.18862488716786 6.19212759828214e-11 PITA -0.382717992487471 2.85392372719143e-07 NA NA NA hsa-miR-589-3p CDC14B 1.16846704028095 5.88525574424201e-11 -1.18862488716786 6.19212759828214e-11 mirMAP -0.326909386043277 4.813791147356e-11 NA NA NA hsa-miR-590-5p CDC14B -0.103355559980404 0.310027295317092 -1.18862488716786 6.19212759828214e-11 miRanda -0.363543336255735 4.96466784460973e-05 NA NA NA hsa-miR-9-5p CDC14B 1.26407994492898 8.53156156439881e-05 -1.18862488716786 6.19212759828214e-11 MirTarget -0.11293009065759 4.78211491074325e-05 NA NA NA hsa-miR-192-5p CDC20 -0.50188499512787 0.00344713341196035 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.257887721839452 0.00747315576024174 NA NA NA hsa-miR-215-5p CDC20 -0.981544773246509 3.14452272477156e-05 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.210290018213749 0.00250187708869541 NA NA NA hsa-miR-23b-3p CDC20 -0.530561587109023 5.43807206823652e-07 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.727746315213922 2.45876854567768e-06 NA NA NA hsa-miR-30a-5p CDC20 -0.626257932887855 0.000108198196973765 4.43876134810924 2.33731245396441e-49 miRNAWalker2_validate -0.686824996889202 5.1373930712059e-12 NA NA NA hsa-miR-29c-3p CDC23 -1.4367517608807 1.61767774905781e-21 0.463635495366116 5.65757358389337e-10 miRNAWalker2_validate -0.137258097204054 2.34594396923131e-09 NA NA NA hsa-let-7b-5p CDC25A -0.959013717270803 5.95127281258643e-11 1.92057841155944 2.96621670231863e-18 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.370009596159664 6.17259890107862e-07 NA NA NA hsa-let-7c-5p CDC25A -1.70744661345097 2.87934212005942e-20 1.92057841155944 2.96621670231863e-18 MirTarget -0.51524439141821 8.99192957021977e-21 25909324 MicroRNA let 7c Inhibits Cell Proliferation and Induces Cell Cycle Arrest by Targeting CDC25A in Human Hepatocellular Carcinoma; The aim of the present study was to determine whether the cell cycle regulator CDC25A is involved in the antitumor effect of let-7c in HCC; The luciferase reporter assay showed that CDC25A was a direct target of let-7c and that let-7c inhibited the expression of CDC25A protein by directly targeting its 3' UTR; In conclusion this study indicates that let-7c suppresses HCC progression possibly by directly targeting the cell cycle regulator CDC25A and indirectly affecting its downstream target molecules progression liver cancer hsa-let-7g-5p CDC25A -0.461294133285527 2.17210114729901e-05 1.92057841155944 2.96621670231863e-18 MirTarget;miRNATAP -0.342243652304718 0.000887619690203727 NA NA NA hsa-miR-193b-3p CDC25A -0.166697207281748 0.272022543069482 1.92057841155944 2.96621670231863e-18 miRNAWalker2_validate -0.243879084457018 0.000987492976723664 NA NA NA hsa-miR-195-5p CDC25A -1.85907534356434 1.47434041676402e-22 1.92057841155944 2.96621670231863e-18 MirTarget;miRNATAP -0.345165924677639 3.04677833261703e-10 NA NA NA hsa-miR-30b-3p CDC25A -0.439724365151373 0.000947180503583586 1.92057841155944 2.96621670231863e-18 MirTarget -0.291072318481627 0.000535860573434097 NA NA NA hsa-miR-424-5p CDC25A -2.62653257498508 2.38997974737991e-50 1.92057841155944 2.96621670231863e-18 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.288771172993147 2.08206643580705e-07 NA NA NA hsa-miR-497-5p CDC25A -1.41371173021948 7.09278946477743e-17 1.92057841155944 2.96621670231863e-18 MirTarget;miRNATAP -0.347445879436172 3.29180016744072e-08 NA NA NA hsa-let-7a-5p CDC25B -0.328069937201898 0.000464344093744051 0.802575978153976 1.68584041760539e-08 miRNAWalker2_validate -0.209265115474151 0.00570595808420343 NA NA NA hsa-miR-148a-3p CDC25B -0.748702855596996 3.93313343865641e-07 0.802575978153976 1.68584041760539e-08 miRNAWalker2_validate;miRNATAP -0.29297329943429 2.56785527501486e-10 25341915 Gene CDC25B might be the target gene of miR-148a according to the results of targetscan; CDC25B may be the target gene of miR-148a that plays a role in tumor suppressor gastric cancer hsa-miR-204-5p CDC25B -0.537962150012103 0.0330895493358849 0.802575978153976 1.68584041760539e-08 miRNATAP -0.160852442555331 4.64874282921328e-09 NA NA NA hsa-miR-26b-5p CDC25B -1.10804856399744 4.14176110314282e-22 0.802575978153976 1.68584041760539e-08 miRNAWalker2_validate -0.283705192321105 1.05482202325139e-06 NA NA NA hsa-miR-27b-3p CDC25B -0.823491817819754 9.20935302746823e-14 0.802575978153976 1.68584041760539e-08 miRNATAP -0.298569829692256 1.30532384058526e-06 NA NA NA hsa-miR-142-3p CDC25C -1.4243101967815 1.92609481097765e-14 4.75334243203995 3.18038389718343e-54 miRanda -0.321598772191666 0.000290480554768757 NA NA NA hsa-miR-142-3p CDC6 -1.4243101967815 1.92609481097765e-14 3.43409755895777 3.54117234711163e-45 miRNAWalker2_validate -0.209613186749947 0.00240261531698926 NA NA NA hsa-miR-199a-5p CDC6 -1.98848462405923 2.0421622713753e-11 3.43409755895777 3.54117234711163e-45 miRanda -0.267112671827499 4.32680677860271e-10 NA NA NA hsa-miR-26a-5p CDC6 -0.963792031426845 1.83360999387345e-22 3.43409755895777 3.54117234711163e-45 miRNAWalker2_validate -0.792267027675385 3.38263043470135e-10 25100863;27158389 Here it is demonstrated that miR26a and miR26b inhibit replication licensing and the proliferation migration and invasion of lung cancer cells by targeting CDC6; The current study suggests that miR26a miR26b and CDC6 and factors regulating their expression represent potential cancer diagnostic and prognostic markers as well as anticancer targets;miR 26a inhibits the proliferation of ovarian cancer cells via regulating CDC6 expression; Bioinformatics analysis revealed Cdc6 was a target gene of miR-26a; dual-luciferase assay and validation assay showed miR-26a could act on the 3'UTR of Cdc6 to regulate Cdc6 expression; These findings suggest that miR-26a may act on the 3'UTR of Cdc6 to regulate Cdc6 expression which then inhibit the proliferation of ovarian cancer cells and induce their apoptosis ; lung cancer;ovarian cancer hsa-miR-3607-3p CDC6 -2.15518588554493 1.03390728226728e-23 3.43409755895777 3.54117234711163e-45 mirMAP -0.421624410789328 1.12661397039956e-13 NA NA NA hsa-miR-101-3p CDC7 -1.48176407980989 3.98950707385704e-31 2.01751950910415 4.47781393592943e-28 miRNAWalker2_validate -0.618519649455716 1.77004294891154e-20 NA NA NA hsa-miR-126-5p CDC7 -0.428561469405594 6.6035585844222e-05 2.01751950910415 4.47781393592943e-28 mirMAP -0.394449488088942 8.64621091696843e-06 NA NA NA hsa-miR-192-5p CDC7 -0.50188499512787 0.00344713341196035 2.01751950910415 4.47781393592943e-28 miRNAWalker2_validate -0.19830872980512 0.000359320910618898 NA NA NA hsa-miR-199a-5p CDC7 -1.98848462405923 2.0421622713753e-11 2.01751950910415 4.47781393592943e-28 MirTarget;miRanda -0.111439193657119 0.000381838786294689 NA NA NA hsa-miR-215-5p CDC7 -0.981544773246509 3.14452272477156e-05 2.01751950910415 4.47781393592943e-28 miRNAWalker2_validate -0.107716575020922 0.00749268034794886 NA NA NA hsa-miR-3065-3p CDC7 -1.04244468016704 4.60743424372577e-05 2.01751950910415 4.47781393592943e-28 MirTarget -0.125795095836249 0.000681186625781064 NA NA NA hsa-miR-335-5p CDC7 -1.61347836513558 1.62757083289711e-19 2.01751950910415 4.47781393592943e-28 MirTarget -0.183857935379035 0.000324011220012664 NA NA NA hsa-miR-3607-3p CDC7 -2.15518588554493 1.03390728226728e-23 2.01751950910415 4.47781393592943e-28 miRNATAP -0.281769617963819 7.78721407023138e-12 NA NA NA hsa-miR-122-5p CDK4 -1.23907441373955 4.42181244888642e-06 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate -0.116994239033144 4.65868213756134e-11 NA NA NA hsa-miR-145-5p CDK4 -1.4795885711477 6.47308361543414e-16 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate;miRTarBase -0.153089865179612 2.59222750442516e-09 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-193b-3p CDK4 -0.166697207281748 0.272022543069482 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate -0.152018771778108 2.97527104247713e-06 NA NA NA hsa-miR-195-5p CDK4 -1.85907534356434 1.47434041676402e-22 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate;miRTarBase -0.181976803431634 3.34979886785679e-14 NA NA NA hsa-let-7a-3p CDK6 -0.568717557554068 1.00947202557819e-07 -0.306731100792328 0.220566158555725 miRNATAP -0.341190533180457 0.00261304127571301 NA NA NA hsa-let-7b-5p CDK6 -0.959013717270803 5.95127281258643e-11 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.21783266130525 0.0075638804735268 NA NA NA hsa-miR-106a-5p CDK6 -0.464229535269236 0.009724944358047 -0.306731100792328 0.220566158555725 mirMAP -0.278977768977315 3.5360030094147e-05 NA NA NA hsa-miR-106b-5p CDK6 0.648901865047099 8.24870862047885e-09 -0.306731100792328 0.220566158555725 mirMAP -0.387039073753013 0.000291052086223802 NA NA NA hsa-miR-141-3p CDK6 -0.354316381352484 0.256996894288799 -0.306731100792328 0.220566158555725 TargetScan;miRNATAP -0.140574578703219 0.000314373241862064 NA NA NA hsa-miR-148b-3p CDK6 0.266862251671911 0.00185271302045935 -0.306731100792328 0.220566158555725 mirMAP -0.757154003587567 1.00024492805767e-07 NA NA NA hsa-miR-16-5p CDK6 -0.398577235179603 0.000101598495180191 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.498098719613092 2.63556306703195e-05 NA NA NA hsa-miR-17-5p CDK6 0.698861147045461 2.43854106455398e-05 -0.306731100792328 0.220566158555725 TargetScan;mirMAP -0.333266464458022 4.3415707047215e-06 NA NA NA hsa-miR-182-5p CDK6 1.96601340710222 4.39326175603633e-09 -0.306731100792328 0.220566158555725 mirMAP -0.10653370302744 0.0027913600827753 NA NA NA hsa-miR-195-5p CDK6 -1.85907534356434 1.47434041676402e-22 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.257207165713251 2.04308440041189e-05 23333942 Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378 respectively gastric cancer hsa-miR-200c-3p CDK6 -0.104675615392797 0.716957669484981 -0.306731100792328 0.220566158555725 mirMAP -0.120071361082355 0.00446253530887124 NA NA NA hsa-miR-20a-5p CDK6 0.854326758727003 5.04256669663285e-07 -0.306731100792328 0.220566158555725 mirMAP -0.336108073900107 1.71022894952879e-06 NA NA NA hsa-miR-20b-5p CDK6 0.457668023910379 0.0285865211936156 -0.306731100792328 0.220566158555725 mirMAP -0.209722611209641 0.000304623744169473 26166554 The transfection of miR-20b into EJ cells induced G1 phase cell cycle arrest via the decreased expression of cyclin D1 CDK2 and CDK6 without affecting another G1 phase cell cycle regulator cyclin E bladder cancer hsa-miR-217 CDK6 1.05797297540381 0.0401026692682422 -0.306731100792328 0.220566158555725 mirMAP -0.175237317848738 1.85593116207955e-14 NA NA NA hsa-miR-224-3p CDK6 1.40770116795301 1.31426607144612e-08 -0.306731100792328 0.220566158555725 mirMAP -0.144127925871375 0.00324265854216547 NA NA NA hsa-miR-26a-5p CDK6 -0.963792031426845 1.83360999387345e-22 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase;miRNATAP -0.450700287383469 0.000126142178652791 26314438 Maxvision immunohistochemistry technique was used to detect the expression level of CDK6 and miR-26a in tissue of 20 ENKTCL cases 10 cases of proliferative lymphadenitis and 10 samples of normal lymph node respectively; The possible role of miR-26a and its target gene CDK6 in genesis and development of ENKTCL were analyzed according to the clinical features of ENKTCL patients; Correlation analysis showed that there was significant negative correlation between miR-26a expression and CDK6 expression r = -0.54 P = 0.04 lymphoma hsa-miR-338-3p CDK6 0.543135607704581 0.00461167747143826 -0.306731100792328 0.220566158555725 mirMAP -0.192008504458263 0.00245323065472347 NA NA NA hsa-miR-340-5p CDK6 -0.00477614531381576 0.968502722773791 -0.306731100792328 0.220566158555725 mirMAP -0.591488535627004 3.30562522942768e-09 NA NA NA hsa-miR-425-5p CDK6 0.585974239943864 2.23063410931402e-05 -0.306731100792328 0.220566158555725 mirMAP -0.371054247671967 2.11644953421021e-05 NA NA NA hsa-miR-452-5p CDK6 1.91544498799508 1.81995982853313e-14 -0.306731100792328 0.220566158555725 mirMAP -0.148489463562499 0.00161659837456088 NA NA NA hsa-miR-497-5p CDK6 -1.41371173021948 7.09278946477743e-17 -0.306731100792328 0.220566158555725 miRNATAP -0.339765827186355 7.37877046693881e-07 NA NA NA hsa-miR-502-3p CDK6 0.659598854007546 3.01316453996423e-08 -0.306731100792328 0.220566158555725 PITA;miRNATAP -0.485378420730533 1.35374384457666e-06 NA NA NA hsa-miR-616-5p CDK6 0.150569904638223 0.402836556304524 -0.306731100792328 0.220566158555725 mirMAP -0.218127986999019 0.00133578296465318 NA NA NA hsa-miR-885-5p CDK6 -0.940994379567566 0.00118729865914986 -0.306731100792328 0.220566158555725 mirMAP -0.312653333627933 1.15118921616921e-14 NA NA NA hsa-miR-92a-3p CDK6 0.212391012518195 0.134292268089997 -0.306731100792328 0.220566158555725 miRNATAP -0.478355192378327 1.89776806005731e-08 NA NA NA hsa-miR-139-5p CDK7 -2.11299531796557 8.16407076415038e-28 0.491707717966495 7.16261478943003e-09 miRanda -0.14310504808171 4.57209013484235e-13 NA NA NA hsa-miR-542-3p CDK7 -1.31351015149842 3.64642423288576e-22 0.491707717966495 7.16261478943003e-09 miRanda -0.177460362938327 8.63098208373725e-10 NA NA NA hsa-miR-590-3p CDK7 -0.473167498047808 1.76652934637227e-05 0.491707717966495 7.16261478943003e-09 miRanda -0.178615786936345 2.1764183873582e-06 NA NA NA hsa-miR-106b-5p CDKN1A 0.648901865047099 8.24870862047885e-09 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.437829469030798 7.84136969105116e-08 NA NA NA hsa-miR-146b-5p CDKN1A 0.419719546795449 0.0457375823220155 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate -0.153153076910721 0.000589578943698456 27602131 During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis progression thyroid cancer hsa-miR-17-5p CDKN1A 0.698861147045461 2.43854106455398e-05 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.29756314131737 8.83846469129569e-08 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-20a-5p CDKN1A 0.854326758727003 5.04256669663285e-07 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.281025701374886 1.92150921220327e-07 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-423-3p CDKN1A 0.302157659154355 0.000669907339064765 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase -0.424628898441296 6.37065354057174e-05 NA NA NA hsa-miR-423-5p CDKN1A 0.698807775906485 1.02195196334612e-11 -0.769088197669944 5.80039048047741e-05 MirTarget -0.370602271693157 3.44327865138196e-05 NA NA NA hsa-miR-503-5p CDKN1A 0.191949282338049 0.268420664548092 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase -0.140430121948101 0.00973159792553366 NA NA NA hsa-miR-93-5p CDKN1A 1.39518349670953 2.84167728726712e-25 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.446611351561038 4.29858025785308e-12 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 0.346451845105263 0.0283255399972342 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate -0.264158154736877 7.79693170645745e-06 NA NA NA hsa-miR-181a-5p CDKN1B 0.250113435902954 0.0551933249319293 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase -0.134068475887496 1.13890102336643e-05 NA NA NA hsa-miR-221-3p CDKN1B 1.12244756358612 3.48370308238012e-13 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.153438831523776 5.56071406720925e-10 20146005;23637992;19953484;23939688;19126397;23967190;17569667;22992757;17721077;20461750 Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression staging;tumorigenesis;progression;;;;;;;;poor survival liver cancer;breast cancer;glioblastoma;breast cancer;melanoma;pancreatic cancer;prostate cancer;breast cancer;glioblastoma;ovarian cancer hsa-miR-222-3p CDKN1B 1.08574156066353 1.76407192348131e-11 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.140430207935426 3.56247154847449e-09 19953484;26912358;24895988;24137356;17569667;27282281;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Besides microvesicle marker characterization we evidenced that miR-222 exosomal expression mostly reflected its abundance in the cells of origin correctly paralleled by repression of its target genes such as p27Kip1 and induction of the PI3K/AKT pathway thus confirming its functional implication in cancer;MiR-222 plays an important role in the tumorigenesis of CC possibly by specifically down-regulating p27Kip1 and PTEN;miR 222 is upregulated in epithelial ovarian cancer and promotes cell proliferation by downregulating P27kip1; miR-222 upregulation induced an enhancement of ovarian cancer cell proliferation potential possibly by downregulating its target P27Kip1; A bioinformatic analysis showed that the 3'-UTR of the P27Kip1 mRNA contained a highly-conserved putative miR-222 binding site; Luciferase reporter assays demonstrated that P27Kip1 was a direct target of miR-222; Consistently there was an inverse correlation between the P27Kip1 and miR-222 expression levels in the ovarian cancer cell lines and tissues;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;miR 222 confers the resistance of breast cancer cells to Adriamycin through suppression of p27kip1 expression; Immunofluorescence showed that miR-222 altered the subcellular location of p27kip1 in nucleus; The results showed that downregulation of miR-222 in MCF-7/Adr increased sensitivity to Adr and Adr-induced apoptosis and arrested the cells in G1 phase accompanied by more expressions of p27kip1 especially in nucleus; Taken together the results found that miR-222 induced Adr-resistance at least in part via suppressing p27kip1 expression and altering its subcellular localization and miR-222 inhibitors could reverse Adr-resistance of breast cancer cells;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;tumorigenesis;;;drug resistance;poor survival glioblastoma;melanoma;cervical and endocervical cancer;ovarian cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-24-3p CDKN1B -0.257825594021966 0.00689742600215061 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRNATAP -0.125575697425242 0.00293958904599202 26847530;26044523 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated;With the bioinformatic method we further identified that p27Kip1 is a direct target of miR-24-3p and its protein level was negatively regulated by miR-24-3p ; prostate cancer;breast cancer hsa-miR-98-5p CDKN1B -0.0487189107419432 0.715911066732548 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate -0.150589087046664 3.98220273478164e-07 NA NA NA hsa-let-7g-5p CDKN2A -0.461294133285527 2.17210114729901e-05 3.99778785884008 5.9152054899506e-37 miRNAWalker2_validate;miRTarBase -0.477507540180217 0.00200442971450149 NA NA NA hsa-miR-125a-5p CDKN2A -0.91219969372382 1.38256246489548e-07 3.99778785884008 5.9152054899506e-37 miRanda -0.535484271218715 1.42706283082069e-08 NA NA NA hsa-miR-125b-5p CDKN2A -1.35767721102906 1.16564617395164e-18 3.99778785884008 5.9152054899506e-37 miRNAWalker2_validate -0.65479078041233 1.86032919550119e-10 23585871 In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b; Conversely treatment of prostate cancer cells with an inhibitor of miR-125b anti-miR-125b resulted in increased expression of p14ARF decreased level of Mdm2 and induction of apoptosis; In addition overexpression of miR-125b in p53-deficient PC3 cells induced down-regulation of p14ARF which leads to increased cell proliferation through a p53-independent manner prostate cancer hsa-miR-455-3p CDKN2A -1.3998033821095 5.72010709806871e-17 3.99778785884008 5.9152054899506e-37 miRNAWalker2_validate -0.424733120907109 9.98850494017421e-06 NA NA NA hsa-miR-126-5p CDKN2B -0.428561469405594 6.6035585844222e-05 1.37191742600332 3.8601745589837e-15 mirMAP -0.331524443678709 4.86520309619025e-05 NA NA NA hsa-miR-144-3p CDKN2B -2.97536914478815 6.00546047309683e-28 1.37191742600332 3.8601745589837e-15 MirTarget -0.165363817374004 2.99908004062454e-08 NA NA NA hsa-miR-382-5p CDKN2B -0.71884748690977 0.00849172229801356 1.37191742600332 3.8601745589837e-15 mirMAP -0.101243601276092 0.00159366073719854 NA NA NA hsa-miR-450b-5p CDKN2B -1.34333360369432 2.68386079790462e-17 1.37191742600332 3.8601745589837e-15 mirMAP -0.177653728760579 0.000849213139863512 NA NA NA hsa-miR-451a CDKN2B -2.1025161666445 6.82555767537341e-17 1.37191742600332 3.8601745589837e-15 MirTarget -0.162386705840671 1.05027558906518e-06 NA NA NA hsa-miR-486-5p CDKN2B -1.77766890600022 2.9071717929922e-14 1.37191742600332 3.8601745589837e-15 miRanda -0.19221239071621 9.16912999787263e-08 NA NA NA hsa-miR-450b-5p CDKN2C -1.34333360369432 2.68386079790462e-17 2.08821471271675 1.38541951717446e-30 miRNATAP -0.407319031602086 4.73616061228169e-13 NA NA NA hsa-miR-542-3p CDKN2C -1.31351015149842 3.64642423288576e-22 2.08821471271675 1.38541951717446e-30 miRanda -0.51609932750582 1.59421631749972e-15 NA NA NA hsa-miR-192-5p CDKN2D -0.50188499512787 0.00344713341196035 0.290727219677442 0.00930996537957609 miRNAWalker2_validate -0.138849298331389 1.02446714787693e-05 NA NA NA hsa-miR-139-5p CHEK1 -2.11299531796557 8.16407076415038e-28 1.37723795528964 1.0573870902276e-18 miRanda -0.413297733218518 4.65960798548544e-31 NA NA NA hsa-miR-195-5p CHEK1 -1.85907534356434 1.47434041676402e-22 1.37723795528964 1.0573870902276e-18 MirTarget;miRNATAP -0.271942240141891 1.81327823892101e-12 25840419 MiR 195 suppresses non small cell lung cancer by targeting CHEK1; We discovered that CHEK1 was a direct target of miR-195 which decreased CHEK1 expression in lung cancer cells lung squamous cell cancer hsa-miR-326 CHEK1 -1.881935132554 1.68087316863775e-21 1.37723795528964 1.0573870902276e-18 miRanda -0.108090613593213 0.00505181638895353 NA NA NA hsa-miR-424-5p CHEK1 -2.62653257498508 2.38997974737991e-50 1.37723795528964 1.0573870902276e-18 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.283677722756659 2.52006692893939e-13 22469983 Suppressed miR 424 expression via upregulation of target gene Chk1 contributes to the progression of cervical cancer; Moreover miR-424 expression levels were inversely correlated with Chk1 and p-Chk1 protein levels in both cervical cancer and normal tissues; Furthermore RNAi-mediated knockdown of Chk1 decreased matrix metalloproteinase 9 expression and phenocopied the tumor suppressive effects of miR-424 in cell models; Taken together our results identify a crucial tumor suppressive role of miR-424 in the progression of cervical cancer at least partly via upreglating the expression of Chk1 and p-Chk1 and suggest that miR-424 might be a candidate of prognostic predictor or an anticancer therapeutic target for cervical cancer patients progression cervical and endocervical cancer hsa-miR-497-5p CHEK1 -1.41371173021948 7.09278946477743e-17 1.37723795528964 1.0573870902276e-18 MirTarget;miRNATAP -0.288964042519174 6.26011681896352e-11 24464213 Checkpoint kinase 1 is negatively regulated by miR 497 in hepatocellular carcinoma; In silico analysis showed that CHEK1 was a candidate target of miR-497 which was previously found to be downregulated in HCC by us; To test whether miR-497 could bind to 3'untranslated region 3'UTR of CHEK1 luciferase reporter assay was conducted; The result revealed that miR-497 could bind to the 3'untranslated region 3'UTR of CHEK1 mRNA; Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1; Finally miR-497 expression was measured in the same 30 HCC samples and the correlation between miR-497 and CHEK1 was analyzed; The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1; Taken together these results demonstrated that CHEK1 was negatively regulated by miR-497 and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC which provided a potential molecular target for HCC therapy liver cancer hsa-miR-511-5p CHEK1 -1.75067415533112 3.37801163573342e-23 1.37723795528964 1.0573870902276e-18 MirTarget -0.25658486157125 8.6821122029758e-10 NA NA NA hsa-miR-542-3p CHEK2 -1.31351015149842 3.64642423288576e-22 0.994881726057935 3.38875860481421e-09 miRanda -0.175087780040737 0.0027946939746593 NA NA NA hsa-miR-324-3p CREBBP 0.260348469146494 0.0506141704203545 -0.198953226514463 0.0138678371438249 miRNAWalker2_validate -0.122219674872295 3.31240083157391e-05 NA NA NA hsa-miR-330-5p CREBBP 0.442231058604099 0.0053308560843808 -0.198953226514463 0.0138678371438249 miRanda -0.10760918491025 1.18364633640293e-05 NA NA NA hsa-miR-769-5p CREBBP 0.219661452072771 0.0333425465750576 -0.198953226514463 0.0138678371438249 miRNAWalker2_validate -0.128844707446474 0.00077006671697313 NA NA NA hsa-miR-27a-3p CUL1 -0.370807380415112 0.00875721956202713 -0.117901782494851 0.10228448838388 miRNAWalker2_validate -0.100449098638373 4.43211535246804e-05 NA NA NA hsa-miR-195-3p DBF4 -1.09375690196245 4.70092324819807e-09 0.924895042673626 1.9008434964275e-15 mirMAP -0.122761280981961 7.99493049317741e-05 NA NA NA hsa-miR-30a-5p DBF4 -0.626257932887855 0.000108198196973765 0.924895042673626 1.9008434964275e-15 MirTarget -0.233029013858222 4.14759152817446e-11 NA NA NA hsa-miR-30e-5p DBF4 -0.627815042856657 3.02205690184908e-10 0.924895042673626 1.9008434964275e-15 MirTarget -0.167793410853246 0.00390640864782242 NA NA NA hsa-miR-3607-3p DBF4 -2.15518588554493 1.03390728226728e-23 0.924895042673626 1.9008434964275e-15 MirTarget;miRNATAP -0.157207609296571 5.40688898105347e-10 NA NA NA hsa-miR-542-3p DBF4 -1.31351015149842 3.64642423288576e-22 0.924895042673626 1.9008434964275e-15 miRanda -0.13261216933844 0.00124728512837269 NA NA NA hsa-let-7a-5p E2F1 -0.328069937201898 0.000464344093744051 3.7552527009539 6.33758772261827e-48 miRNAWalker2_validate -0.451450780631698 0.00302856569262514 NA NA NA hsa-miR-10a-5p E2F1 -1.47723516092033 7.27299669108673e-16 3.7552527009539 6.33758772261827e-48 miRNAWalker2_validate -0.587411489648547 3.96759482211711e-16 NA NA NA hsa-miR-126-3p E2F1 -0.652349350198172 9.37876280344772e-09 3.7552527009539 6.33758772261827e-48 miRNAWalker2_validate -0.743805651122085 8.75836838490869e-10 NA NA NA hsa-miR-203a-3p E2F1 -1.34362479226226 8.69374456831293e-05 3.7552527009539 6.33758772261827e-48 miRTarBase -0.114980068350657 0.00507064286258392 25216463 miR 203 is a direct transcriptional target of E2F1 and causes G1 arrest in esophageal cancer cells; Here we reveal that miR-203 is regulated by E2F1 an important transcription factor that can induce cell proliferation by controlling cell cycle progression; We found that miR-203 expression was induced by cisplatin which also induced E2F1 protein accumulation in esophageal squamous cell carcinoma ESCC cell lines; miR-203 expression was elevated upon activation of ectopic E2F1 whereas this induction was abolished when the E2F1 gene was silenced; Moreover with luciferase reporter assays and chromatin immunoprecipitation ChIP assays we demonstrated that E2F1 transactivates miR-203 by directly binding to the miR-203 gene promoter; Taken together our data show that cancer-related miR-203 is a novel transcriptional target of E2F1 and that it regulates cell cycle arrest by participating in a feedback loop with E2F1 progression esophageal cancer hsa-miR-223-3p E2F1 -0.996015099450654 2.19036606105147e-07 3.7552527009539 6.33758772261827e-48 miRNAWalker2_validate;miRTarBase -0.266387219262254 0.000239971547562287 20029046 Cell cycle regulator E2F1 and microRNA 223 comprise an autoregulatory negative feedback loop in acute myeloid leukemia; In this report we demonstrate that during granulopoiesis microRNA-223 targets E2F1; E2F1 protein was up-regulated in miR-223 null mice; Our study supports a molecular network involving miR-223 C/EBPalpha and E2F1 as major components of the granulocyte differentiation program which is deregulated in AML differentiation acute myeloid leukemia hsa-miR-23b-3p E2F1 -0.530561587109023 5.43807206823652e-07 3.7552527009539 6.33758772261827e-48 miRNAWalker2_validate -0.507514171013405 0.00013254227259674 NA NA NA hsa-let-7b-5p E2F2 -0.959013717270803 5.95127281258643e-11 2.82262179260901 6.90997126839698e-33 MirTarget -0.371169195680849 7.55477650894117e-06 NA NA NA hsa-let-7c-5p E2F2 -1.70744661345097 2.87934212005942e-20 2.82262179260901 6.90997126839698e-33 MirTarget -0.605641654221748 3.1290897462579e-23 NA NA NA hsa-let-7g-5p E2F2 -0.461294133285527 2.17210114729901e-05 2.82262179260901 6.90997126839698e-33 MirTarget -0.497410799147229 1.3134926743002e-05 NA NA NA hsa-miR-125a-5p E2F2 -0.91219969372382 1.38256246489548e-07 2.82262179260901 6.90997126839698e-33 MirTarget -0.198307329285792 0.00532736298626263 NA NA NA hsa-miR-125b-5p E2F2 -1.35767721102906 1.16564617395164e-18 2.82262179260901 6.90997126839698e-33 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.799704959655455 4.93523419395096e-28 22999819 miR 125b regulates the proliferation of glioblastoma stem cells by targeting E2F2; This study demonstrated that miR-125b plays important roles in regulating the proliferation of GSCs by directly targeting E2F2 glioblastoma hsa-miR-130a-3p E2F2 -1.53229181736223 2.91460363624872e-19 2.82262179260901 6.90997126839698e-33 miRNATAP -0.37915978729184 3.98791790226269e-08 NA NA NA hsa-miR-204-5p E2F2 -0.537962150012103 0.0330895493358849 2.82262179260901 6.90997126839698e-33 mirMAP -0.268912601041465 3.53614087792816e-08 NA NA NA hsa-miR-214-5p E2F2 -1.91497809567701 3.92955595018147e-12 2.82262179260901 6.90997126839698e-33 MirTarget;miRNATAP -0.177970455644998 6.17643330181357e-05 NA NA NA hsa-miR-22-3p E2F2 -0.628561621952407 1.33755558015523e-09 2.82262179260901 6.90997126839698e-33 miRNAWalker2_validate -1.17700583503314 1.01771679679134e-25 NA NA NA hsa-miR-26a-5p E2F2 -0.963792031426845 1.83360999387345e-22 2.82262179260901 6.90997126839698e-33 miRNATAP -0.61643534337946 2.52870127087977e-07 26458859 MiR 26a enhances the sensitivity of gastric cancer cells to cisplatin by targeting NRAS and E2F2; Our results suggest that miR-26a can improve the sensitivity of GC cells to cisplatin-based chemotherapies through targeting NRAS and E2F2 and provide the first evidence of the potential utility of miR-26a as a sensitizer in chemotherapy for GC gastric cancer hsa-miR-26b-5p E2F2 -1.10804856399744 4.14176110314282e-22 2.82262179260901 6.90997126839698e-33 miRNATAP -0.706148859696605 3.56790944939986e-12 NA NA NA hsa-miR-30c-1-3p E2F2 -1.39373825444537 6.39912952911454e-17 2.82262179260901 6.90997126839698e-33 MirTarget -0.217389717898255 0.00294054014962802 NA NA NA hsa-miR-30c-2-3p E2F2 -1.39811819128587 3.66376924527665e-15 2.82262179260901 6.90997126839698e-33 MirTarget -0.414124894866312 5.37875240613613e-10 NA NA NA hsa-miR-326 E2F2 -1.881935132554 1.68087316863775e-21 2.82262179260901 6.90997126839698e-33 MirTarget -0.164698864060144 0.0066331250825117 NA NA NA hsa-miR-3614-5p E2F2 -1.66311416965679 1.26647181860439e-15 2.82262179260901 6.90997126839698e-33 mirMAP -0.266827358982619 4.59687042303078e-06 NA NA NA hsa-miR-378a-3p E2F2 -1.1920055906972 3.23538871733634e-10 2.82262179260901 6.90997126839698e-33 MirTarget -0.254203475754501 7.50780324109086e-05 NA NA NA hsa-miR-378c E2F2 -1.54028191929038 2.70131554214866e-15 2.82262179260901 6.90997126839698e-33 MirTarget -0.352049468131631 7.74346148574887e-09 NA NA NA hsa-miR-101-3p E2F3 -1.48176407980989 3.98950707385704e-31 0.679179223655044 3.98775697474768e-07 miRNAWalker2_validate -0.408210251322335 1.00202902205119e-18 NA NA NA hsa-miR-125b-5p E2F3 -1.35767721102906 1.16564617395164e-18 0.679179223655044 3.98775697474768e-07 miRNAWalker2_validate;miRTarBase;miRNATAP -0.299646522641145 6.99209821825783e-14 22523546;20549700 Moreover we demonstrated that the E2F3 was a direct target of miR-125b in breast cancer cells;E2F3 which was critical for G1/S transition and was overexpressed in most of poor-differentiated bladder cancers was identified as a target of miR-125b by luciferase assay; Introduction of miR-125b could reduce the expression of E2F3 protein but not the E2F3 mRNA ; breast cancer;bladder cancer hsa-miR-148a-3p E2F3 -0.748702855596996 3.93313343865641e-07 0.679179223655044 3.98775697474768e-07 miRNATAP -0.286439887340466 4.00406934916546e-11 NA NA NA hsa-miR-194-5p E2F3 -0.292530160992458 0.0996141384876663 0.679179223655044 3.98775697474768e-07 miRNATAP -0.170298851438611 4.06002835298195e-06 NA NA NA hsa-miR-195-5p E2F3 -1.85907534356434 1.47434041676402e-22 0.679179223655044 3.98775697474768e-07 miRNAWalker2_validate;miRTarBase;miRNATAP -0.115038038576163 0.000460113206577871 22217655 We identified E2F3 and CCND3 as functional downstream targets of miR-195 in glioblastoma cells glioblastoma hsa-miR-455-5p E2F3 -0.270546034117157 0.0581320703577731 0.679179223655044 3.98775697474768e-07 miRanda -0.124732321929516 0.00724459816666969 NA NA NA hsa-miR-497-5p E2F3 -1.41371173021948 7.09278946477743e-17 0.679179223655044 3.98775697474768e-07 miRNATAP -0.132647905668094 0.000398904982300106 NA NA NA hsa-miR-23b-3p E2F4 -0.530561587109023 5.43807206823652e-07 0.52624092062884 1.99370091261106e-11 miRNAWalker2_validate -0.134293182725783 0.000238709419026524 NA NA NA hsa-let-7a-2-3p E2F5 -1.19254913456912 1.47840144520517e-08 0.730554652437087 0.000401890915871585 MirTarget -0.236368683960896 5.41845122818239e-07 NA NA NA hsa-let-7a-5p E2F5 -0.328069937201898 0.000464344093744051 0.730554652437087 0.000401890915871585 MirTarget;TargetScan;miRNATAP -0.388478797116257 0.00033740750885559 NA NA NA hsa-let-7b-5p E2F5 -0.959013717270803 5.95127281258643e-11 0.730554652437087 0.000401890915871585 miRNAWalker2_validate;MirTarget;miRNATAP -0.21870636746378 0.00120101767570817 NA NA NA hsa-let-7c-5p E2F5 -1.70744661345097 2.87934212005942e-20 0.730554652437087 0.000401890915871585 MirTarget -0.228095461415917 1.04748751614991e-05 NA NA NA hsa-let-7g-3p E2F5 -1.14091000722297 8.32736018188759e-19 0.730554652437087 0.000401890915871585 MirTarget;miRNATAP -0.30841341167427 3.97782527999337e-05 NA NA NA hsa-let-7g-5p E2F5 -0.461294133285527 2.17210114729901e-05 0.730554652437087 0.000401890915871585 MirTarget;miRNATAP -0.281429469337869 0.0024764517018281 NA NA NA hsa-miR-1-3p E2F5 -0.658358049898834 0.0102519519479171 0.730554652437087 0.000401890915871585 MirTarget -0.10626442102449 0.00712838434232038 NA NA NA hsa-miR-192-5p E2F5 -0.50188499512787 0.00344713341196035 0.730554652437087 0.000401890915871585 miRNAWalker2_validate -0.165569976807069 0.00489423335560786 NA NA NA hsa-miR-3653-3p E2F5 -1.77536656608462 3.05384496241509e-24 0.730554652437087 0.000401890915871585 MirTarget -0.158966973078016 0.00370031639799338 NA NA NA hsa-miR-378a-3p E2F5 -1.1920055906972 3.23538871733634e-10 0.730554652437087 0.000401890915871585 miRNAWalker2_validate -0.193048272442212 0.000208784950864508 NA NA NA hsa-miR-339-5p EP300 0.280589919878422 0.0355660282647215 -0.200382966790078 0.0704945874784134 miRanda -0.157358640254018 9.53193539159902e-05 NA NA NA hsa-miR-342-3p EP300 -0.319677314984262 0.0449813878317147 -0.200382966790078 0.0704945874784134 MirTarget;PITA;miRanda;miRNATAP -0.112632997815777 0.000853079257159957 NA NA NA hsa-let-7b-5p ESPL1 -0.959013717270803 5.95127281258643e-11 1.87757313624794 3.45937181689806e-13 MirTarget -0.23517623264085 0.00664457544852015 NA NA NA hsa-let-7c-5p ESPL1 -1.70744661345097 2.87934212005942e-20 1.87757313624794 3.45937181689806e-13 MirTarget -0.45417008213531 3.14084416633658e-12 NA NA NA hsa-let-7g-5p ESPL1 -0.461294133285527 2.17210114729901e-05 1.87757313624794 3.45937181689806e-13 MirTarget -0.322418161806339 0.00684942045031933 NA NA NA hsa-miR-10a-5p ESPL1 -1.47723516092033 7.27299669108673e-16 1.87757313624794 3.45937181689806e-13 miRNAWalker2_validate -0.317292085793379 2.76056702570866e-06 NA NA NA hsa-miR-148b-3p GADD45A 0.266862251671911 0.00185271302045935 -1.18438355981419 2.04421074395418e-12 MirTarget -0.53346134919236 5.63428920227987e-08 NA NA NA hsa-miR-301a-3p GADD45A 0.842910511640361 1.43446467417891e-06 -1.18438355981419 2.04421074395418e-12 MirTarget;miRNATAP -0.429003570363722 3.99121895415153e-21 NA NA NA hsa-miR-324-5p GADD45A 0.372933276649782 0.00592280066108904 -1.18438355981419 2.04421074395418e-12 miRanda -0.518772278708973 4.69587473312867e-18 NA NA NA hsa-miR-331-5p GADD45A 0.213759185384356 0.120772399478646 -1.18438355981419 2.04421074395418e-12 PITA;miRNATAP -0.430187189615618 9.35766561284238e-13 NA NA NA hsa-miR-421 GADD45A 0.941856334694081 6.12167297054077e-08 -1.18438355981419 2.04421074395418e-12 miRanda -0.399146316642953 4.95904028323981e-18 NA NA NA hsa-miR-429 GADD45A -1.40262535827285 6.93982219171912e-05 -1.18438355981419 2.04421074395418e-12 miRNATAP -0.147502825337448 3.86036414101022e-10 NA NA NA hsa-miR-454-3p GADD45A 0.668749059573955 2.42670710515113e-06 -1.18438355981419 2.04421074395418e-12 MirTarget;miRNATAP -0.336757743574097 6.70178941647605e-09 NA NA NA hsa-miR-500a-5p GADD45A 0.797906382969156 9.15958171084226e-08 -1.18438355981419 2.04421074395418e-12 MirTarget;miRNATAP -0.380400118148641 2.78037785058895e-12 NA NA NA hsa-miR-500b-5p GADD45A 0.79864276129988 8.80934488971056e-08 -1.18438355981419 2.04421074395418e-12 MirTarget -0.380385448906191 2.83925177775155e-12 NA NA NA hsa-miR-502-5p GADD45A 0.892373335336396 1.7122694518919e-06 -1.18438355981419 2.04421074395418e-12 MirTarget;PITA;miRNATAP -0.211861066175866 2.8783697640044e-06 NA NA NA hsa-miR-324-3p GADD45B 0.260348469146494 0.0506141704203545 -2.18315196507153 1.75545549044514e-24 MirTarget;miRNATAP -0.489337683262879 2.36967761576399e-09 NA NA NA hsa-miR-181a-5p GADD45G 0.250113435902954 0.0551933249319293 -1.8078339802055 7.96436141465682e-13 miRNAWalker2_validate -0.541338211358986 1.77478587318898e-08 NA NA NA hsa-miR-320a GADD45G 0.325285936317332 0.0221415065838824 -1.8078339802055 7.96436141465682e-13 miRanda -0.463178177312941 1.50939059891479e-07 NA NA NA hsa-miR-320b GADD45G 0.0919652022571331 0.607981643616945 -1.8078339802055 7.96436141465682e-13 miRanda -0.326039418271956 3.89750223667119e-06 NA NA NA hsa-miR-421 GADD45G 0.941856334694081 6.12167297054077e-08 -1.8078339802055 7.96436141465682e-13 miRanda -0.556853667845386 1.3427336009444e-15 NA NA NA hsa-miR-429 GADD45G -1.40262535827285 6.93982219171912e-05 -1.8078339802055 7.96436141465682e-13 miRNATAP -0.116320782056629 0.00122761610181022 NA NA NA hsa-miR-502-5p GADD45G 0.892373335336396 1.7122694518919e-06 -1.8078339802055 7.96436141465682e-13 miRNATAP -0.210942956888822 0.00206657080418105 NA NA NA hsa-miR-29b-3p GSK3B -0.348885528720517 0.0121441341291285 0.11314223970595 0.0774493616232908 miRTarBase;miRNATAP -0.106852381323571 1.32787261855633e-06 NA NA NA hsa-miR-342-3p GSK3B -0.319677314984262 0.0449813878317147 0.11314223970595 0.0774493616232908 miRanda -0.106989468297279 2.44530655156687e-08 NA NA NA hsa-miR-30a-5p HDAC1 -0.626257932887855 0.000108198196973765 0.310222809591839 0.000149745117698607 miRNAWalker2_validate -0.146313546021308 1.38072373678855e-09 NA NA NA hsa-let-7b-3p HDAC2 -1.21979213547786 5.84575581584603e-17 0.159768613336722 0.0632725636896593 mirMAP -0.122366992013411 8.0964121762738e-06 NA NA NA hsa-miR-125b-2-3p HDAC2 -1.6609436107315 5.71534047969855e-18 0.159768613336722 0.0632725636896593 mirMAP -0.102822311849734 6.33678598956499e-07 NA NA NA hsa-miR-139-5p MAD2L1 -2.11299531796557 8.16407076415038e-28 2.12271197949055 2.5877373631959e-30 miRanda -0.59204072876179 1.2497576446289e-44 NA NA NA hsa-miR-139-5p MCM2 -2.11299531796557 8.16407076415038e-28 2.386213124759 2.3135357405317e-32 miRanda -0.60342281675185 7.72277609982919e-38 NA NA NA hsa-miR-192-5p MCM3 -0.50188499512787 0.00344713341196035 1.38245898490982 6.59458276841991e-26 miRNAWalker2_validate -0.118151752469436 0.00283190558585697 NA NA NA hsa-miR-26b-5p MCM3 -1.10804856399744 4.14176110314282e-22 1.38245898490982 6.59458276841991e-26 miRNAWalker2_validate -0.337133110078096 1.47085794375846e-09 NA NA NA hsa-miR-450b-5p MCM3 -1.34333360369432 2.68386079790462e-17 1.38245898490982 6.59458276841991e-26 miRNATAP -0.217971388429723 8.89582939084555e-08 NA NA NA hsa-miR-486-5p MCM4 -1.77766890600022 2.9071717929922e-14 1.32614408881812 5.23855543390612e-15 miRanda -0.135110848198039 0.000125083316969585 NA NA NA hsa-miR-542-3p MCM5 -1.31351015149842 3.64642423288576e-22 1.34044307943415 2.17000754715776e-22 miRanda -0.279862800712507 1.04514523632503e-08 NA NA NA hsa-miR-192-5p MCM6 -0.50188499512787 0.00344713341196035 1.68713872113643 5.47744551594301e-30 miRNAWalker2_validate -0.129535871289776 0.00415957553139546 NA NA NA hsa-miR-30c-1-3p MCM6 -1.39373825444537 6.39912952911454e-17 1.68713872113643 5.47744551594301e-30 MirTarget -0.128319123277048 0.00481540286180515 NA NA NA hsa-miR-30c-2-3p MCM6 -1.39811819128587 3.66376924527665e-15 1.68713872113643 5.47744551594301e-30 MirTarget -0.269887567350998 7.24016535492575e-11 NA NA NA hsa-let-7b-5p MCM7 -0.959013717270803 5.95127281258643e-11 1.28544451959494 2.38346141988918e-22 miRNAWalker2_validate -0.247616423212068 3.20929332495545e-08 NA NA NA hsa-miR-148b-5p MDM2 0.295777213389605 0.0255749859535438 -0.36172823237465 0.000468851696238999 mirMAP -0.126111944341174 0.000979992057303969 NA NA NA hsa-miR-181a-2-3p MDM2 -0.232005557505191 0.236404981276858 -0.36172823237465 0.000468851696238999 mirMAP -0.112388243613946 1.17734645194124e-05 NA NA NA hsa-miR-23a-3p MDM2 -0.178875974460574 0.135978273540604 -0.36172823237465 0.000468851696238999 mirMAP -0.134193900580038 0.00153318263298883 NA NA NA hsa-miR-25-3p MDM2 0.633062563556379 3.68686354834328e-10 -0.36172823237465 0.000468851696238999 miRNAWalker2_validate;miRTarBase -0.177207631206492 0.0003251640137327 NA NA NA hsa-miR-500a-5p MDM2 0.797906382969156 9.15958171084226e-08 -0.36172823237465 0.000468851696238999 mirMAP -0.143618881374049 1.52638427528629e-05 NA NA NA hsa-miR-589-3p MDM2 1.16846704028095 5.88525574424201e-11 -0.36172823237465 0.000468851696238999 mirMAP -0.123089784785846 1.18949322878182e-05 NA NA NA hsa-miR-423-5p MYC 0.698807775906485 1.02195196334612e-11 -1.53204423111148 3.53987625613016e-10 miRNAWalker2_validate -0.328206409340035 0.00484551582134482 NA NA NA hsa-miR-24-3p PCNA -0.257825594021966 0.00689742600215061 0.966521187541277 2.13251268175503e-18 miRNAWalker2_validate -0.174598229125149 0.00323913466605276 NA NA NA hsa-miR-26b-5p PCNA -1.10804856399744 4.14176110314282e-22 0.966521187541277 2.13251268175503e-18 miRNAWalker2_validate -0.274512184414973 2.31662573425547e-09 NA NA NA hsa-miR-30a-5p PCNA -0.626257932887855 0.000108198196973765 0.966521187541277 2.13251268175503e-18 miRNAWalker2_validate -0.140104186770926 4.44986190016326e-05 NA NA NA hsa-miR-542-3p PCNA -1.31351015149842 3.64642423288576e-22 0.966521187541277 2.13251268175503e-18 miRanda;miRNATAP -0.256334358356727 2.8017131374182e-11 NA NA NA hsa-let-7b-5p PLK1 -0.959013717270803 5.95127281258643e-11 3.58200503456178 1.01936996392609e-41 miRNAWalker2_validate -0.508374715415364 7.45675351637923e-08 NA NA NA hsa-miR-100-5p PLK1 -0.783576782126614 0.000219958772262677 3.58200503456178 1.01936996392609e-41 miRNAWalker2_validate;miRTarBase -0.609428308452059 1.35398975085084e-21 23842624;23151088;22246341;25537513;22120675;21636267 In HCC tissues miR-100 expression was inversely correlated with the expression of plk1 protein r = -0.418; P = 0.029; Therefore downregulation of miR-100 was correlated with progressive pathological feature and poor prognosis in HCC patients and miR-100 could function as a tumor suppressor by targeting plk1;MicroRNA 100 is a potential molecular marker of non small cell lung cancer and functions as a tumor suppressor by targeting polo like kinase 1; By using microRNA miR target prediction algorithms we identified miR-100 that might potentially bind the 3'-untranslated region of PLK1 transcripts; The purpose of this study was to investigate the roles of miR-100 and its association with PLK1 in NSCLC development; Finally the effects of miR-100 expression on growth apoptosis and cell cycle of NSCLC cells by posttranscriptionally regulating PLK1 expression were determined; Meanwhile miR-100 mimics could significantly inhibit PLK1 mRNA and protein expression and reduce the luciferase activity of a PLK1 3' untranslated region-based reporter construct in A549 cells; Furthermore small interfering RNA siRNA-mediated PLK1 downregulation could mimic the effects of miR-100 mimics while PLK1 overexpression could partially rescue the phenotypical changes of NSCLC cells induced by miR-100 mimics; Our findings indicate that low miR-100 may be a poor prognostic factor for NSCLC patients and functions as a tumor suppressor by posttranscriptionally regulating PLK1 expression;Together these results suggest that low miR-100 expression may be an independent poor prognostic factor and miR-100 can function as a tumor suppressor by targeting PLK1 in human EOCs;Here we show that miR-100 inhibits maintenance and expansion of BrCSCs in basal-like cancer through Polo-like kinase1 Plk1 down-regulation;MiR 100 resensitizes docetaxel resistant human lung adenocarcinoma cells SPC A1 to docetaxel by targeting Plk1; Knock-down of Plk1 which was a direct target of miR-100 yielded similar effects as that of ectopic miR-100 expression; The inverse correlation between miR-100 and Plk1 expression was also detected in nude mice SPC-A1/DTX tumor xenografts and clinical lung adenocarcinoma tissues and was proved to be related with the in vivo response to docetaxel; Thus our results suggested that down-regulation of miR-100 could lead to Plk1 over-expression and eventually to docetaxel chemoresistance of human lung adenocarcinoma;Reduced miR 100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein; Through modulating miR-100 expression using miR-100 inhibitor or mimic in vitro cell growth cycle and apoptosis were tested separately by MTT or flow cytometry and meanwhile Polo-like kinase1 PLK1 mRNA and protein expressions were detected by qRT-PCR and immunoblotting; The expression of PLK1 in 125 cervical tissues was also examined by immunohistochemical staining and the correlation between miR-100 and PLK1 expression in the same tissues was analysed; The modulation of miR-100 expression remarkably influenced cell proliferation cycle and apoptosis as well as the level of PLK1 protein but not mRNA in vitro experiments; PLK1 expression was negatively correlated with miR-100 expression in CIN3 and cervical cancer tissues; The reduced miR-100 expression participates in the development of cervical cancer at least partly through loss of inhibition to target gene PLK1 which probably occurs in a relative late phase of carcinogenesis progression;worse prognosis;;;;drug resistance;tumorigenesis liver cancer;lung squamous cell cancer;ovarian cancer;breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-486-5p PLK1 -1.77766890600022 2.9071717929922e-14 3.58200503456178 1.01936996392609e-41 miRanda -0.373122790226321 1.30117161322891e-10 NA NA NA hsa-miR-505-5p PLK1 -0.768282569967001 1.14662846595407e-05 3.58200503456178 1.01936996392609e-41 MirTarget -0.372253566265304 3.64975232604557e-06 NA NA NA hsa-miR-874-3p PLK1 -1.07043026298634 2.09564226589678e-07 3.58200503456178 1.01936996392609e-41 miRNAWalker2_validate -0.236755899629093 0.000522664320516165 NA NA NA hsa-miR-101-5p PRKDC -1.0634649894055 4.39116719827902e-16 0.698911772501382 1.43592514036518e-09 miRNAWalker2_validate;miRTarBase -0.211569429493643 4.55776294994216e-07 22014955 Although ectopic miR-101 efficiently decreased the ATM and DNA-PKcs levels and increased the radiosensitization level in H1299 H1975 and A549 cells it did not change the levels of the miR-101 targets or radiosensitivity in H157 cells lung squamous cell cancer hsa-let-7b-5p PTTG1 -0.959013717270803 5.95127281258643e-11 3.76246853388833 8.36103372767795e-44 miRNAWalker2_validate -0.651597525972495 1.331355553891e-11 NA NA NA hsa-miR-26a-5p PTTG1 -0.963792031426845 1.83360999387345e-22 3.76246853388833 8.36103372767795e-44 miRNAWalker2_validate -1.0151550901517 2.62899523096093e-13 NA NA NA hsa-let-7a-2-3p RAD21 -1.19254913456912 1.47840144520517e-08 0.535354921594023 4.14289891566247e-08 MirTarget -0.118211773772855 1.3714836978455e-07 NA NA NA hsa-let-7b-3p RAD21 -1.21979213547786 5.84575581584603e-17 0.535354921594023 4.14289891566247e-08 miRNATAP -0.102996560841551 0.00121189622450311 NA NA NA hsa-miR-139-5p RAD21 -2.11299531796557 8.16407076415038e-28 0.535354921594023 4.14289891566247e-08 miRanda -0.143025976760077 4.04070937342955e-10 NA NA NA hsa-miR-26b-3p RAD21 -1.25503731613309 4.45390890119649e-27 0.535354921594023 4.14289891566247e-08 MirTarget -0.231269810927681 1.58406394994566e-09 NA NA NA hsa-miR-30e-3p RAD21 -1.20845647373031 3.10263633528203e-25 0.535354921594023 4.14289891566247e-08 MirTarget -0.184103670374031 2.12767912805574e-06 NA NA NA hsa-miR-106b-5p RB1 0.648901865047099 8.24870862047885e-09 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.271965886856172 3.89284256525718e-06 NA NA NA hsa-miR-130b-3p RB1 0.687190305027546 0.000109068658562255 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate -0.115537577137445 0.00222803653320818 NA NA NA hsa-miR-17-5p RB1 0.698861147045461 2.43854106455398e-05 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.155748489310193 0.00011314998657351 NA NA NA hsa-miR-20a-5p RB1 0.854326758727003 5.04256669663285e-07 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.144090309816489 0.000231958406673791 NA NA NA hsa-miR-21-5p RB1 1.51084295924322 1.54551622173259e-34 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate -0.130479458630801 0.00994795669328339 NA NA NA hsa-miR-93-5p RB1 1.39518349670953 2.84167728726712e-25 -0.431695598029404 0.00179279020523563 miRNAWalker2_validate;MirTarget;miRNATAP -0.243228053890559 2.22933663222982e-07 NA NA NA hsa-miR-122-5p RBL1 -1.23907441373955 4.42181244888642e-06 1.01562013245512 3.92765086455926e-07 MirTarget -0.233942086696873 4.05290204360084e-11 NA NA NA hsa-miR-106a-5p RBL2 -0.464229535269236 0.009724944358047 -0.988381422306337 9.82564074336519e-12 MirTarget;miRNATAP -0.117107890118936 0.00367936194107588 24045973 Among many miR-106a predicated target genes p130 RBL2 an retinoblastoma Rb tumor suppressor family member was not only confirmed as a specific target of miR-106a but also related to tumor growth and differentiation; The importance of mir-106a and RBL2 was further demonstrated in vivo in which SKOV3 cells overexpressing miR-106a formed poorly differentiated carcinomas and had reduced RBL2 levels differentiation ovarian cancer hsa-miR-106b-5p RBL2 0.648901865047099 8.24870862047885e-09 -0.988381422306337 9.82564074336519e-12 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.467612098017658 4.18657885507219e-14 NA NA NA hsa-miR-17-5p RBL2 0.698861147045461 2.43854106455398e-05 -0.988381422306337 9.82564074336519e-12 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.343656172428148 2.71737625593314e-16 NA NA NA hsa-miR-20a-5p RBL2 0.854326758727003 5.04256669663285e-07 -0.988381422306337 9.82564074336519e-12 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.264295882999639 1.60446558088321e-10 NA NA NA hsa-miR-335-3p RBL2 -0.275199247483111 0.106629165248783 -0.988381422306337 9.82564074336519e-12 MirTarget -0.114041268766779 0.00743241946989908 NA NA NA hsa-miR-421 RBL2 0.941856334694081 6.12167297054077e-08 -0.988381422306337 9.82564074336519e-12 miRanda -0.251090760557798 5.73687778774342e-10 NA NA NA hsa-miR-93-5p RBL2 1.39518349670953 2.84167728726712e-25 -0.988381422306337 9.82564074336519e-12 MirTarget;miRNATAP -0.408615196615982 9.43227929469049e-17 NA NA NA hsa-miR-139-5p RBX1 -2.11299531796557 8.16407076415038e-28 0.27440095690029 0.00162986680164289 miRanda -0.10537307725778 2.07264839791855e-07 NA NA NA hsa-miR-450b-5p SKP1 -1.34333360369432 2.68386079790462e-17 0.220350434523828 0.00211239594525225 miRNATAP -0.104764813664882 5.36557139667176e-07 NA NA NA hsa-miR-7-1-3p SKP1 -0.569105143287799 1.85297665041424e-05 0.220350434523828 0.00211239594525225 MirTarget;mirMAP -0.109724018574561 2.33101260912259e-05 NA NA NA hsa-miR-21-5p SKP2 1.51084295924322 1.54551622173259e-34 0.422595289761893 0.0200334942687184 miRNAWalker2_validate;MirTarget -0.199555879642397 0.00262016397392478 NA NA NA hsa-miR-30b-5p SKP2 -0.54056186708617 2.44688760798341e-05 0.422595289761893 0.0200334942687184 MirTarget;miRNATAP -0.240712598784266 0.000463630582388284 NA NA NA hsa-miR-455-5p SMAD2 -0.270546034117157 0.0581320703577731 0.315604980822302 9.76507196938151e-07 miRanda -0.126014730396316 7.82261975485323e-09 NA NA NA hsa-let-7g-3p SMAD3 -1.14091000722297 8.32736018188759e-19 0.313849246286883 0.00280528337161062 mirMAP -0.100129949934208 0.00883180340844614 NA NA NA hsa-miR-142-5p SMAD3 -1.44575553250033 1.18797044680592e-13 0.313849246286883 0.00280528337161062 PITA -0.125868007472141 6.14850270977044e-07 NA NA NA hsa-miR-155-5p SMAD3 0.0108524225071784 0.95651331033505 0.313849246286883 0.00280528337161062 miRNAWalker2_validate -0.143616291871971 1.61818308217997e-08 27626488 Here we demonstrated that TGF-β1 elevated the expression of miR-155 in colorectal cancer cells through SMAD3 and SMAD4 colorectal cancer hsa-miR-1976 SMAD3 -0.431472440543166 0.00324575010550946 0.313849246286883 0.00280528337161062 MirTarget -0.177460759581402 2.8140086065009e-07 NA NA NA hsa-miR-29b-2-5p SMAD3 -0.223258653034772 0.084306146519938 0.313849246286883 0.00280528337161062 mirMAP -0.124265788567886 0.00177731205069881 NA NA NA hsa-miR-326 SMAD3 -1.881935132554 1.68087316863775e-21 0.313849246286883 0.00280528337161062 miRanda -0.121886887629074 7.48502793476032e-07 NA NA NA hsa-miR-424-5p SMAD3 -2.62653257498508 2.38997974737991e-50 0.313849246286883 0.00280528337161062 MirTarget -0.105846554796035 3.45471115916176e-05 27655675 miR 424 5p promotes proliferation of gastric cancer by targeting Smad3 through TGF β signaling pathway; QRT-PCR was used to determine the expression levels of miR-424-5p and Smad3; Luciferase reporter assay and western blotting were used to prove that Smad3 was one of the direct targets of miR-424-5p; In addition luciferase reporter assay and western blotting assay revealed that Smad3 was a direct target of miR-424-5p; Over-expression of Smad3 could partially reverse the effects of miR-424-5p on GC cell proliferation; Our study further revealed that miR-424-5p could inhibit TGF-β signaling pathway by Smad3 gastric cancer hsa-miR-29b-2-5p SMAD4 -0.223258653034772 0.084306146519938 -0.30976054630181 7.55983294717014e-05 MirTarget -0.1066651080199 0.000315915680961711 NA NA NA hsa-miR-34a-5p SMAD4 1.03694231790865 5.74864019540594e-14 -0.30976054630181 7.55983294717014e-05 miRNATAP -0.106481148933391 7.14459232465546e-05 22750848 Mechanistically in addition to its direct regulation of platelet-derived growth factor receptor-alpha PDGFRA promoter enrichment analysis of context likelihood of relatedness-inferred mRNA nodes established miR-34a as a novel regulator of a SMAD4 transcriptional network; Specifically in glioblastoma within the context of the global network promoter enrichment analysis of network edges uncovered a novel regulation of TGF-β signaling via a Smad4 transcriptomic network by miR-34a glioblastoma hsa-miR-500a-5p SMAD4 0.797906382969156 9.15958171084226e-08 -0.30976054630181 7.55983294717014e-05 mirMAP -0.104579732494838 3.18281872137464e-05 NA NA NA hsa-miR-181a-5p SMC1A 0.250113435902954 0.0551933249319293 0.0651833474337025 0.510370716253497 mirMAP -0.117542893899761 0.00146146139664929 NA NA NA hsa-miR-181c-5p SMC1A -0.00639394152050299 0.969127212255928 0.0651833474337025 0.510370716253497 mirMAP -0.102787280067137 0.000412049730773496 NA NA NA hsa-miR-193b-3p SMC3 -0.166697207281748 0.272022543069482 0.203644948628179 0.0850721957839735 miRNAWalker2_validate -0.129842117232487 0.000624818169450842 NA NA NA hsa-miR-30b-5p STAG1 -0.54056186708617 2.44688760798341e-05 -0.178198619776885 0.243967920887864 MirTarget -0.20850853639894 0.00030033116479739 NA NA NA hsa-miR-181a-5p STAG2 0.250113435902954 0.0551933249319293 -0.0714198831818473 0.421841747970601 miRNAWalker2_validate -0.136157385997054 3.70034379856412e-05 NA NA NA hsa-miR-21-5p STAG2 1.51084295924322 1.54551622173259e-34 -0.0714198831818473 0.421841747970601 miRNAWalker2_validate;MirTarget;miRNATAP -0.137742528039037 1.73808146164746e-05 NA NA NA hsa-miR-342-3p STAG2 -0.319677314984262 0.0449813878317147 -0.0714198831818473 0.421841747970601 miRanda -0.10214432208538 0.000151861811120363 NA NA NA hsa-let-7f-1-3p TFDP2 -0.704017923073338 7.84883628944999e-07 -0.0754175393834782 0.416101839729762 mirMAP -0.106691567048515 0.000739096343618018 NA NA NA hsa-miR-23a-3p TFDP2 -0.178875974460574 0.135978273540604 -0.0754175393834782 0.416101839729762 mirMAP -0.109370175763973 0.00374300397027472 NA NA NA hsa-miR-27a-3p TFDP2 -0.370807380415112 0.00875721956202713 -0.0754175393834782 0.416101839729762 mirMAP -0.101203939020943 0.00145452232576687 NA NA NA hsa-miR-29b-3p TFDP2 -0.348885528720517 0.0121441341291285 -0.0754175393834782 0.416101839729762 mirMAP -0.130627056894638 5.03437535065681e-05 NA NA NA hsa-miR-342-3p TFDP2 -0.319677314984262 0.0449813878317147 -0.0754175393834782 0.416101839729762 miRNATAP -0.108002069558564 0.000123333656246695 NA NA NA hsa-miR-139-5p TGFB1 -2.11299531796557 8.16407076415038e-28 0.0791820105139553 0.6886056388594 miRanda -0.165335310650966 0.000359864512900183 NA NA NA hsa-miR-101-5p TGFB2 -1.0634649894055 4.39116719827902e-16 0.529934214584709 0.036730862150581 mirMAP -0.351880994764185 0.000113322446416492 NA NA NA hsa-miR-107 TGFB2 0.241662461253461 0.0170788899505389 0.529934214584709 0.036730862150581 miRanda -0.614314042069378 4.68765266846655e-07 NA NA NA hsa-miR-148a-3p TGFB2 -0.748702855596996 3.93313343865641e-07 0.529934214584709 0.036730862150581 MirTarget;miRNATAP -0.661431979951743 1.43220896593668e-16 NA NA NA hsa-miR-193a-3p TGFB2 -0.117675551589896 0.309391958208578 0.529934214584709 0.036730862150581 MirTarget;miRanda;miRNATAP -0.366329573810208 0.000659116520736421 NA NA NA hsa-miR-193b-3p TGFB2 -0.166697207281748 0.272022543069482 0.529934214584709 0.036730862150581 MirTarget;miRNATAP -0.318957683478265 9.08737208626492e-05 NA NA NA hsa-miR-33a-3p TGFB2 -0.683025859637737 1.01106097756419e-05 0.529934214584709 0.036730862150581 MirTarget;mirMAP -0.292910263006269 0.00021856118592367 NA NA NA hsa-miR-362-3p TGFB2 0.814007335895913 2.00108618836568e-09 0.529934214584709 0.036730862150581 miRanda -0.235702914624554 0.00870288726391307 NA NA NA hsa-miR-548b-3p TGFB2 -0.0203576084717134 0.930819503166956 0.529934214584709 0.036730862150581 MirTarget -0.251413034966269 2.19240613879876e-06 NA NA NA hsa-miR-616-5p TGFB2 0.150569904638223 0.402836556304524 0.529934214584709 0.036730862150581 mirMAP -0.373291858655729 4.43049125036333e-08 NA NA NA hsa-miR-618 TGFB2 0.13539895350352 0.517145913114103 0.529934214584709 0.036730862150581 MirTarget;miRNATAP -0.323812991792447 9.41184108923406e-08 NA NA NA hsa-miR-151a-5p TGFB3 0.484893436232714 4.8995594459361e-06 -0.549558180002636 0.00349778458392359 MirTarget -0.493845417648935 6.80157351019684e-09 NA NA NA hsa-miR-122-5p TP53 -1.23907441373955 4.42181244888642e-06 -0.163823087975333 0.268013799235932 miRNATAP -0.104401376732605 6.76735273123785e-05 23221562;22235305 Loss of miR-122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G1-modulated P53 activity.;Surprisingly miR-122 overexpression decreased the sensitivity to the chemotherapy-induced apoptosis via a signaling circuit involving the activation of Akt and inhibition of p53 ; liver cancer;lymphoma hsa-miR-25-3p TP53 0.633062563556379 3.68686354834328e-10 -0.163823087975333 0.268013799235932 miRNAWalker2_validate -0.198477612132015 0.00480268903724675 22431589 The results define miR-25 and -32 as positive regulators of p53 underscoring their role in tumorigenesis in glioblastoma tumorigenesis glioblastoma hsa-miR-139-5p TTK -2.11299531796557 8.16407076415038e-28 4.46695225538541 3.90954998066403e-48 miRanda -1.02753150497536 5.11914670343957e-45 NA NA NA hsa-miR-455-3p TTK -1.3998033821095 5.72010709806871e-17 4.46695225538541 3.90954998066403e-48 miRNATAP -0.457661482122706 1.91508543926432e-06 NA NA NA hsa-miR-140-3p WEE1 0.546012239205329 4.84578261098777e-11 -1.08704483398787 1.08882602430213e-14 PITA;miRNATAP -0.269624323625237 0.00142013204313983 NA NA NA hsa-miR-107 YWHAB 0.241662461253461 0.0170788899505389 0.173432047741129 0.000884243127746855 miRanda -0.108037306925883 1.47087762859095e-05 NA NA NA hsa-miR-148a-3p YWHAB -0.748702855596996 3.93313343865641e-07 0.173432047741129 0.000884243127746855 MirTarget;miRNATAP -0.132820922282045 4.13292521804208e-16 NA NA NA hsa-miR-186-5p YWHAE -0.060103391373687 0.535288717081205 -0.389050776848833 1.87949073175552e-07 miRNAWalker2_validate -0.127528287333916 0.000863275542227347 NA NA NA hsa-miR-192-3p YWHAH -0.6401165114007 0.000266272092403814 0.381424797266773 9.9564983661589e-07 MirTarget -0.123473061996178 7.45881423647248e-09 NA NA NA hsa-miR-139-5p YWHAQ -2.11299531796557 8.16407076415038e-28 0.105106147496931 0.185320360738181 MirTarget;PITA;miRanda;miRNATAP -0.134903753655919 8.26465948813414e-14 NA NA NA hsa-miR-140-5p YWHAQ -0.220106443070144 0.0140713197101242 0.105106147496931 0.185320360738181 miRanda -0.114458361490446 0.00840912415391188 NA NA NA hsa-miR-328-3p YWHAQ -0.547466865311955 6.8359574451572e-06 0.105106147496931 0.185320360738181 miRNAWalker2_validate -0.117573761953222 0.000175415891769642 NA NA NA hsa-miR-497-5p YWHAQ -1.41371173021948 7.09278946477743e-17 0.105106147496931 0.185320360738181 MirTarget;miRNATAP -0.10611692853337 9.41242986957199e-07 NA NA NA hsa-miR-193b-3p YWHAZ -0.166697207281748 0.272022543069482 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.215846083623554 1.05246240233955e-09 NA NA NA hsa-miR-195-3p YWHAZ -1.09375690196245 4.70092324819807e-09 0.521348507448096 2.46588555709085e-06 MirTarget -0.123410355780779 1.86763692080896e-05 NA NA NA hsa-miR-22-3p YWHAZ -0.628561621952407 1.33755558015523e-09 0.521348507448096 2.46588555709085e-06 MirTarget -0.422402725731077 3.08582185459414e-17 NA NA NA hsa-miR-28-5p YWHAZ -0.430964830599964 2.67367257252925e-06 0.521348507448096 2.46588555709085e-06 PITA;miRanda;miRNATAP -0.378439288562354 7.80189740644095e-11 NA NA NA hsa-miR-30a-5p YWHAZ -0.626257932887855 0.000108198196973765 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate;MirTarget;miRNATAP -0.137637850164864 3.55605716079781e-05 NA NA NA hsa-miR-30c-5p YWHAZ -0.432111642767632 0.000159764714931007 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.217149970191143 4.21662119138978e-06 24519092 Involvement of miR 30c in resistance to doxorubicin by regulating YWHAZ in breast cancer cells; Together our findings provided evidence that miR-30c was one of the important miRNAs in doxorubicin resistance by regulating YWHAZ in the breast cancer cell line MCF-7/ADR drug resistance breast cancer hsa-miR-30e-5p YWHAZ -0.627815042856657 3.02205690184908e-10 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.512830738492514 1.08747499679871e-23 NA NA NA hsa-miR-328-3p YWHAZ -0.547466865311955 6.8359574451572e-06 0.521348507448096 2.46588555709085e-06 MirTarget -0.184846832867037 2.98681633637662e-05 NA NA NA hsa-miR-340-3p YWHAZ -0.329393899588902 0.00329627776838916 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate -0.145643740083093 0.00280561227887093 NA NA NA hsa-miR-374a-3p YWHAZ -0.207171354826929 0.0623494608399903 0.521348507448096 2.46588555709085e-06 mirMAP -0.243786914759101 6.00089907045834e-07 NA NA NA hsa-miR-378a-3p YWHAZ -1.1920055906972 3.23538871733634e-10 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate -0.229761242047093 2.06324870493417e-17 NA NA NA hsa-miR-451a YWHAZ -2.1025161666445 6.82555767537341e-17 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.125160799099819 8.46499537092129e-10 26885274 miR 451 regulates FoxO3 nuclear accumulation through Ywhaz in human colorectal cancer; In this study expressions of miR-451 and Ywhaz in CRC tissues and adjacent normal tissues were detected by quantitative real-time PCR qRT-PCR and immunohistochemistry respectively; Ywhaz-3'UTR luciferase reporter assay confirmed Ywhaz as a direct target gene of miR-451; miR-451 may inhibit the colon cancer growth in vitro and in vivo likely through directly targeting Ywhaz and indirectly regulating the nuclear accumulation of FoxO3 colorectal cancer hsa-miR-455-3p YWHAZ -1.3998033821095 5.72010709806871e-17 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate -0.163253715474292 1.350262159478e-07 NA NA NA hsa-miR-505-5p YWHAZ -0.768282569967001 1.14662846595407e-05 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.138532797418727 5.93157816903527e-06 NA NA NA hsa-miR-576-5p YWHAZ -0.377452008285847 0.00470735657343984 0.521348507448096 2.46588555709085e-06 mirMAP -0.244889551272521 1.02393670706879e-09 NA NA NA hsa-miR-664a-3p YWHAZ 0.485807855553031 0.000732553249798598 0.521348507448096 2.46588555709085e-06 mirMAP -0.120325216996267 0.00142367135354135 NA NA NA hsa-miR-7-1-3p YWHAZ -0.569105143287799 1.85297665041424e-05 0.521348507448096 2.46588555709085e-06 mirMAP -0.154608457268063 0.000140054345497285 NA NA NA