miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-27a-3p APAF1 0.388496361379095 1.42373532086331e-05 -0.124144138683158 0.0608129314395015 miRNATAP -0.119009261831353 2.41438306531375e-06 NA NA NA hsa-miR-181a-5p ATM 0.635863607630183 6.72486918157744e-11 -0.753520785022149 8.14866377341274e-18 miRNAWalker2_validate;miRTarBase;MirTarget -0.212080382902989 6.50305312230929e-12 21102523;23656790;24531888;27150990;26113450 Ataxia telangiectasia mutated ATM a target gene of miR-181 exhibited reduced expression in mammospheres and upon TGF-β treatment;We report that miR-181a and miR-181b were overexpressed in more aggressive breast cancers and their expression correlates inversely with ATM levels;Ataxia-telangiectasia mutation ATM was predicted as a target gene of miR-181a with bioinformatics analysis and was verified by lucifersae reporter assay; A luciferase reporter assay demonstrated that ATM was a direct target of miR-181a miR-181a mimics transfection down regulated ATM mRNA and protein expression; There was inverse correlation between miR-181a and ATM protein expression in gastric cancer and normal gastric tissues; Our study demonstrates that over-expression of miR-181a might be involved in development of gastric cancer by promoting proliferation and inhibiting apoptosis probably through directly targeting ATM miR-181a modulation may be a potential strategy for the development of miRNA-based therapy of gastric cancer;MiR 181a Promotes Proliferation of Human Acute Myeloid Leukemia Cells by Targeting ATM; Dual luciferase reporter gene assay showed that miR-181a significantly suppressed the reporter gene activity containing ATM 3'-UTR by about 56.8% P < 0.05 but it didn't suppress the reporter gene activity containing 3'-UTR ATM mutation; Western blot showed that miR-181a significantly downregulated the expression of ATM in human leukemia cells; It is also found that miR-181a was significantly increased in AML which showed a negative correlation with ATM expression; miR-181a promotes cell proliferation in AML by regulating the tumor suppressor ATM thus it plays the role as oncogene in pathogenesis of AML;miR 181a promotes G1/S transition and cell proliferation in pediatric acute myeloid leukemia by targeting ATM; Pediatric AML patients and healthy controls were enrolled and the expression of miR-181a and ataxia telangiectasia mutated ATM in tissues were examined using quantitative PCR; Moreover cell proliferation and cell cycle were evaluated in several cell lines HL60 NB4 and K562 by using flow cytometry after transfected with miR-181a mimics and inhibitors or ATM siRNA and control siRNA; Finally ATM as the potential target protein of miR-181a was examined; We found that miR-181a was significantly increased in pediatric AML which showed an inverse association with ATM expression; Luciferase activity of the reporter construct identified ATM as the direct molecular target of miR-181a; The results revealed novel mechanism through which miR-181a regulates G1/S transition and cell proliferation in pediatric AML by regulating the tumor suppressor ATM providing insights into the molecular mechanism in pediatric AML ;;;; breast cancer;breast cancer;gastric cancer;acute myeloid leukemia;acute myeloid leukemia hsa-miR-181b-5p ATM 1.22749257795094 1.21998198830919e-27 -0.753520785022149 8.14866377341274e-18 MirTarget -0.217803705013907 3.99505534670018e-17 21102523;23656790 Ataxia telangiectasia mutated ATM a target gene of miR-181 exhibited reduced expression in mammospheres and upon TGF-β treatment;We report that miR-181a and miR-181b were overexpressed in more aggressive breast cancers and their expression correlates inversely with ATM levels ; breast cancer;breast cancer hsa-miR-181d-5p ATM 0.782618466877349 1.2821116358212e-09 -0.753520785022149 8.14866377341274e-18 MirTarget -0.119716083515509 3.28179905476897e-07 NA NA NA hsa-miR-18a-5p ATM 0.969066793715868 1.69069796425892e-08 -0.753520785022149 8.14866377341274e-18 miRNAWalker2_validate;miRTarBase;MirTarget -0.116309277677122 3.16867464269194e-11 23857602;23437304;25963391;23229340 Furthermore we used antisense oligonucleotides against micro RNAs miRNA or miRNA overexpression plasmids to study the role of miR-18a and -106a on ATM expression; Furthermore we identified that ERα activates miR-18a and -106a to downregulate ATM expression; We reveal a novel mechanism involving ERα and miR-18a and -106a regulation of ATM in breast cancer;MicroRNA 18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer; Through in silico search the 3'UTR of Ataxia telangiectasia mutated ATM contains a conserved miR-18a binding site; Expression of ATM was down-regulated in CRC tumors p<0.0001 and inversely correlated with miR-18a expression r = -0.4562 p<0.01; This was further confirmed by the down-regulation of ATM protein by miR-18a; As ATM is a key enzyme in DNA damage repair we evaluated the effect of miR-18a on DNA double-strand breaks; miR-18a attenuates cellular repair of DNA double-strand breaks by directly suppressing ATM a key enzyme in DNA damage repair;However the upregulation of miR-18a suppressed the level of ataxia-telangiectasia mutated and attenuated DNA double-strand break repair after irradiation which re-sensitized the cervical cancer cells to radiotherapy by promoting apoptosis;MicroRNA 18a upregulates autophagy and ataxia telangiectasia mutated gene expression in HCT116 colon cancer cells; Previous studies showed that certain microRNAs including miR-18a potentially regulate ATM in cancer cells; However the mechanisms behind the modulation of ATM by miR-18a remain to be elucidated in colon cancer cells; In the present study we explored the impact of miR-18a on the autophagy process and ATM expression in HCT116 colon cancer cells; Western blotting and luciferase assays were implemented to explore the impact of miR-18a on ATM gene expression in HCT116 cells; Moreover miR-18a overexpression led to the upregulation of ATM expression and suppression of mTORC1 activity; Results of the present study pertaining to the role of miR-18a in regulating autophagy and ATM gene expression in colon cancer cells revealed a novel function for miR-18a in a critical cellular event and on a crucial gene with significant impacts in cancer development progression treatment and in other diseases ;;;progression breast cancer;colorectal cancer;cervical and endocervical cancer;colon cancer hsa-miR-21-5p ATM 2.32007501846389 3.08394781855554e-137 -0.753520785022149 8.14866377341274e-18 mirMAP -0.230974664847653 3.0596374789676e-17 26289851 MiR-21 is an oncomiR that is overexpressed in nearly all cancers including ATC; Hence suppression of miR-21 could pave the way for ATC therapy thyroid cancer hsa-miR-140-3p ATR -1.25844395244141 5.29222651039892e-33 -0.182962371066174 0.00500352198780384 PITA -0.137096483152858 1.22829525488745e-11 NA NA NA hsa-miR-361-5p ATR -0.0996867547900457 0.095428250126326 -0.182962371066174 0.00500352198780384 miRanda -0.243676561927317 9.34381314288267e-11 NA NA NA hsa-miR-374a-5p ATR -0.239709888246804 0.00309819667153627 -0.182962371066174 0.00500352198780384 mirMAP -0.105234691946044 0.000143287219478725 NA NA NA hsa-miR-365a-3p BAX -1.45032725192851 2.37888098061118e-37 1.31138725140704 3.67717824400239e-48 miRNAWalker2_validate -0.197311099125654 4.24250463560438e-13 24216611 MiR 365 induces gemcitabine resistance in pancreatic cancer cells by targeting the adaptor protein SHC1 and pro apoptotic regulator BAX drug resistance pancreatic cancer hsa-let-7a-2-3p BBC3 -1.30646060964956 1.05363588067753e-14 1.65385724902316 1.48973116107111e-30 MirTarget -0.327549602963543 1.24995827027139e-29 NA NA NA hsa-let-7g-3p BBC3 0.5854327663695 0.000371711766083627 1.65385724902316 1.48973116107111e-30 MirTarget;miRNATAP -0.254397379759528 7.37724438920585e-17 NA NA NA hsa-miR-125a-5p BBC3 -0.635694700253268 3.6703235721638e-11 1.65385724902316 1.48973116107111e-30 miRNATAP -0.347024152717018 3.49069577985272e-11 NA NA NA hsa-miR-125b-5p BBC3 -1.94393269763542 2.78180543229264e-58 1.65385724902316 1.48973116107111e-30 miRNAWalker2_validate;miRTarBase -0.362160606295851 5.96237927857909e-21 25184537 Thus far two of these target genes BBC3 and NEU1 that are tumor suppressor genes but not yet studied in PDAC appear to be functional targets of miR-125b since knockdown of miR125b caused their up regulation pancreatic cancer hsa-miR-139-5p BBC3 -3.25846663863766 2.62018989415003e-112 1.65385724902316 1.48973116107111e-30 miRNATAP -0.224614533926346 1.3398029460868e-13 NA NA NA hsa-miR-140-5p BBC3 -0.755971041589807 9.68060922934542e-14 1.65385724902316 1.48973116107111e-30 miRNATAP -0.473628085409644 2.04784854852423e-22 NA NA NA hsa-miR-143-3p BBC3 -1.23248181631499 4.74160976678504e-28 1.65385724902316 1.48973116107111e-30 miRNATAP -0.217374027351567 7.85719613622932e-07 NA NA NA hsa-miR-150-5p BBC3 -0.832049526091211 3.45299201139224e-05 1.65385724902316 1.48973116107111e-30 miRNATAP -0.129873375742996 2.64339316057827e-07 NA NA NA hsa-miR-222-3p BBC3 -0.429279381480379 0.00103945569781115 1.65385724902316 1.48973116107111e-30 miRNAWalker2_validate;miRNATAP -0.296482501565522 1.39480853031015e-14 20813046 MiR 221 and miR 222 target PUMA to induce cell survival in glioblastoma poor survival glioblastoma hsa-miR-24-3p BBC3 -0.120123317289941 0.21432376743243 1.65385724902316 1.48973116107111e-30 miRNATAP -0.391129070844288 9.30844315993004e-14 NA NA NA hsa-miR-29a-5p BBC3 0.334183773952055 0.00445137265870197 1.65385724902316 1.48973116107111e-30 MirTarget;miRNATAP -0.165777172917072 0.000147170000473594 NA NA NA hsa-miR-324-3p BBC3 0.404877731996915 0.000340036423920834 1.65385724902316 1.48973116107111e-30 PITA -0.363538564773094 3.36815787329759e-16 NA NA NA hsa-miR-345-5p BBC3 0.639728356429512 0.00013070715719672 1.65385724902316 1.48973116107111e-30 miRNATAP -0.228990908761044 2.67029525819491e-14 NA NA NA hsa-miR-532-3p BBC3 -0.395473145042459 0.00686714288452761 1.65385724902316 1.48973116107111e-30 PITA;miRNATAP -0.350419893952163 6.65206801184912e-25 NA NA NA hsa-let-7a-5p CASP3 -0.210286642041636 0.0072546234707717 0.666045797044402 6.39452182888629e-31 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.195644898270621 4.6747877969906e-14 NA NA NA hsa-let-7b-5p CASP3 -0.54215483448257 7.21347617597858e-08 0.666045797044402 6.39452182888629e-31 MirTarget;miRNATAP -0.135377392684109 1.39837315047849e-11 NA NA NA hsa-let-7c-5p CASP3 -1.9415540533505 9.55250745007793e-48 0.666045797044402 6.39452182888629e-31 MirTarget -0.131994349405243 7.44021418353714e-21 NA NA NA hsa-miR-101-3p CASP3 -0.126035286271986 0.145997832808138 0.666045797044402 6.39452182888629e-31 MirTarget -0.205647208491522 1.01890278591706e-18 NA NA NA hsa-miR-139-5p CASP3 -3.25846663863766 2.62018989415003e-112 0.666045797044402 6.39452182888629e-31 miRanda -0.133754697836399 1.82907069570434e-29 NA NA NA hsa-miR-195-3p CASP3 -1.49582726452416 6.53261924105147e-40 0.666045797044402 6.39452182888629e-31 MirTarget -0.161373451028419 9.65654022351954e-22 NA NA NA hsa-miR-30a-5p CASP3 -0.392489994825279 0.00954894667761676 0.666045797044402 6.39452182888629e-31 miRNATAP -0.146653827176832 2.4661703600601e-29 NA NA NA hsa-miR-30c-5p CASP3 -0.393222674580066 8.06961465717306e-05 0.666045797044402 6.39452182888629e-31 miRNATAP -0.111784978428745 4.21945016044375e-08 NA NA NA hsa-miR-20a-5p CASP8 0.44979911120222 0.000602386964327228 0.126270388614871 0.0614764679436649 MirTarget -0.120887766356647 3.653106895295e-12 NA NA NA hsa-miR-17-5p CASP9 0.599531757298529 1.07318660415906e-05 0.0553003248250161 0.30285132191595 TargetScan -0.101631873053047 1.40876150138688e-14 NA NA NA hsa-let-7b-5p CCNB1 -0.54215483448257 7.21347617597858e-08 2.41596782139125 1.64230288731479e-82 miRNAWalker2_validate -0.394935930328456 1.73247776867955e-17 NA NA NA hsa-miR-139-5p CCNB1 -3.25846663863766 2.62018989415003e-112 2.41596782139125 1.64230288731479e-82 miRanda -0.48632264255974 6.28227602164649e-78 NA NA NA hsa-miR-140-5p CCNB1 -0.755971041589807 9.68060922934542e-14 2.41596782139125 1.64230288731479e-82 miRanda -0.151189526798117 0.00114311498286806 NA NA NA hsa-let-7a-5p CCNB2 -0.210286642041636 0.0072546234707717 3.43292983356506 4.14076853090613e-90 miRNAWalker2_validate -0.621192649278321 7.7996632083381e-14 NA NA NA hsa-let-7b-5p CCNB2 -0.54215483448257 7.21347617597858e-08 3.43292983356506 4.14076853090613e-90 miRNAWalker2_validate -0.541022811667963 1.91496765651017e-17 NA NA NA hsa-let-7c-5p CCNB2 -1.9415540533505 9.55250745007793e-48 3.43292983356506 4.14076853090613e-90 miRNAWalker2_validate -0.663845253967336 3.73074325919323e-53 NA NA NA hsa-let-7i-5p CCND1 -0.333419326258177 3.56960810786801e-08 0.789100261201456 1.48424202389562e-06 miRNATAP -0.759700962013673 2.31065071397228e-16 NA NA NA hsa-miR-106a-5p CCND1 0.574594373206545 0.000145868284001732 0.789100261201456 1.48424202389562e-06 MirTarget;miRNATAP -0.174359830876255 2.65007775906387e-06 NA NA NA hsa-miR-106b-5p CCND1 1.22477789913175 2.31787213143326e-32 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.258644062815507 8.24041501542474e-07 NA NA NA hsa-miR-142-3p CCND1 1.53951237846104 2.06769936282034e-17 0.789100261201456 1.48424202389562e-06 miRanda -0.111966511365121 0.000240262100008297 23619912 Transfection of miR-142-3p mimics in colon cancer cells downregulated cyclin D1 expression induced G1 phase cell cycle arrest and elevated the sensitivity of the cells to 5-fluorouracil colon cancer hsa-miR-142-5p CCND1 1.16509303143593 1.96641162209073e-13 0.789100261201456 1.48424202389562e-06 PITA -0.210173449112337 1.63510921192815e-09 NA NA NA hsa-miR-150-5p CCND1 -0.832049526091211 3.45299201139224e-05 0.789100261201456 1.48424202389562e-06 mirMAP -0.187252742453937 1.34917625164856e-11 NA NA NA hsa-miR-155-5p CCND1 1.09766314984151 4.55650930885814e-15 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate -0.298681895389198 2.32439253453393e-14 26955820 MicroRNA 155 expression inversely correlates with pathologic stage of gastric cancer and it inhibits gastric cancer cell growth by targeting cyclin D1 staging gastric cancer hsa-miR-15b-5p CCND1 0.788659356634696 1.13678211092534e-11 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.125463191460891 0.00921827566968511 NA NA NA hsa-miR-17-5p CCND1 0.599531757298529 1.07318660415906e-05 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.23795787679729 6.72139201694027e-09 26431674 Bioinformatics Prediction and In Vitro Analysis Revealed That miR 17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells; In this study using bioinformatic analyses miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score; After lentiviral transduction of miR-17 to the target cells gene expression analysis showed decreased expression of CCND1 gene breast cancer hsa-miR-186-5p CCND1 -0.426987301460247 1.04129380549315e-05 0.789100261201456 1.48424202389562e-06 mirMAP -0.494988902443749 5.18517439622754e-18 NA NA NA hsa-miR-19a-3p CCND1 0.817507952171848 3.76285090469625e-08 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;miRNATAP -0.216023850578105 8.24189814452405e-09 25985117 Moreover miR-19a might play inhibitory roles in HCC malignancy via regulating Cyclin D1 expression liver cancer hsa-miR-19b-1-5p CCND1 0.647704962003062 3.07687667945597e-07 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase -0.206802040114629 3.03348057195458e-06 NA NA NA hsa-miR-19b-3p CCND1 0.139124391010031 0.255658553872934 0.789100261201456 1.48424202389562e-06 miRNATAP -0.249444343540709 5.93160032007176e-08 NA NA NA hsa-miR-20a-5p CCND1 0.44979911120222 0.000602386964327228 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.218045690926451 3.58399990050218e-07 NA NA NA hsa-miR-23b-3p CCND1 -0.363202401123049 0.000265305319764878 0.789100261201456 1.48424202389562e-06 miRNATAP -0.2085521275068 0.000237936656088415 NA NA NA hsa-miR-29a-3p CCND1 -0.699539666833076 1.13935003139957e-13 0.789100261201456 1.48424202389562e-06 mirMAP -0.358623457755512 1.12263469726765e-09 NA NA NA hsa-miR-340-5p CCND1 1.0857159627596 4.94471062546756e-24 0.789100261201456 1.48424202389562e-06 mirMAP -0.157803294541288 0.00210629660665075 NA NA NA hsa-miR-374a-5p CCND1 -0.239709888246804 0.00309819667153627 0.789100261201456 1.48424202389562e-06 MirTarget -0.556352001430099 7.67413515717595e-16 27191497 microRNA 374a suppresses colon cancer progression by directly reducing CCND1 to inactivate the PI3K/AKT pathway; Furthermore luciferase reporter assays confirmed that miR-374a could directly reduce CCND1; We examined miR-374a levels by in situ hybridization and its correlation with CCND1 expression in CRC tumor tissues; High miR-374a expression with low level of CCND1 was protective factor in CRC; Together these findings indicate that miR-374a inactivates the PI3K/AKT axis by inhibiting CCND1 suppressing of colon cancer progression progression colon cancer hsa-miR-374b-5p CCND1 -0.466781429946153 1.17238311619116e-08 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;MirTarget -0.531193112029599 5.3445494840887e-15 NA NA NA hsa-miR-424-5p CCND1 0.420789584199235 0.00480101660479489 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.165346668007668 1.17175782770906e-05 NA NA NA hsa-miR-495-3p CCND1 -1.67685437612894 1.70634429769052e-34 0.789100261201456 1.48424202389562e-06 MirTarget -0.2655760978611 1.39010362325337e-11 NA NA NA hsa-miR-511-5p CCND1 -2.27545475897362 3.84085514050558e-36 0.789100261201456 1.48424202389562e-06 MirTarget -0.21938803198833 1.27831310404617e-13 NA NA NA hsa-miR-589-3p CCND1 0.0263457630961427 0.866577983997705 0.789100261201456 1.48424202389562e-06 MirTarget -0.116809863299678 0.00155105242627909 NA NA NA hsa-miR-590-3p CCND1 1.27438512890514 1.03164723481469e-23 0.789100261201456 1.48424202389562e-06 mirMAP -0.221641713536578 8.94498108920728e-07 NA NA NA hsa-miR-9-5p CCND1 0.734772328078424 0.00261982843636383 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate -0.162121975003827 1.12937727530253e-12 NA NA NA hsa-miR-92a-3p CCND1 -0.342420481092807 0.000732275969501111 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate -0.355039027931141 1.3665845638516e-10 NA NA NA hsa-miR-93-5p CCND1 1.01788600209314 1.24312921618126e-21 0.789100261201456 1.48424202389562e-06 miRNAWalker2_validate;MirTarget;miRNATAP -0.160469090487963 0.00197437840989499 NA NA NA hsa-miR-942-5p CCND1 0.0274758353801279 0.842368857038259 0.789100261201456 1.48424202389562e-06 MirTarget -0.261175156275166 1.39422778172566e-10 NA NA NA hsa-let-7a-3p CCND2 0.466622567891891 3.70886257943079e-07 -1.32381712777542 1.10807863083966e-33 mirMAP -0.215275364862757 3.63993039775257e-07 20418948 MicroRNA let 7a inhibits proliferation of human prostate cancer cells in vitro and in vivo by targeting E2F2 and CCND2 prostate cancer hsa-let-7e-5p CCND2 0.261471269338985 0.00191077095550257 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.168033346737038 0.000309648297817392 NA NA NA hsa-miR-106a-5p CCND2 0.574594373206545 0.000145868284001732 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.117204496646304 5.04333413242193e-06 NA NA NA hsa-miR-106b-5p CCND2 1.22477789913175 2.31787213143326e-32 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.324304654054346 9.75129599972944e-20 NA NA NA hsa-miR-130b-5p CCND2 1.0573161199584 2.55177349228739e-12 -1.32381712777542 1.10807863083966e-33 mirMAP -0.113060251982174 8.9777676148938e-06 NA NA NA hsa-miR-141-3p CCND2 2.99777540204168 1.26370498493024e-78 -1.32381712777542 1.10807863083966e-33 MirTarget;TargetScan -0.300723945575078 8.35441899721039e-47 NA NA NA hsa-miR-151a-3p CCND2 0.3530403796941 3.45015092968892e-05 -1.32381712777542 1.10807863083966e-33 mirMAP -0.17767569994557 0.000108302525662849 NA NA NA hsa-miR-15a-5p CCND2 0.812445324157528 4.50263417099822e-20 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.382989347535057 1.85935181542133e-19 NA NA NA hsa-miR-15b-5p CCND2 0.788659356634696 1.13678211092534e-11 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.200863767053961 1.18885859507843e-09 NA NA NA hsa-miR-16-5p CCND2 0.46835991343556 1.91125356547776e-06 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRNATAP -0.126011969570663 0.00149555991801246 NA NA NA hsa-miR-17-5p CCND2 0.599531757298529 1.07318660415906e-05 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.218141508896336 9.56625930755479e-15 NA NA NA hsa-miR-182-5p CCND2 2.36408084103818 2.60011457600281e-72 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.333755066801542 7.98233999507297e-38 NA NA NA hsa-miR-183-5p CCND2 2.95681814393872 1.09965777344285e-92 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.301857921065621 1.16754966176673e-39 NA NA NA hsa-miR-185-5p CCND2 0.482334746589337 3.68475879959415e-09 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.252091965164496 1.07493887045353e-07 NA NA NA hsa-miR-191-5p CCND2 0.831652724468215 1.98847993606895e-12 -1.32381712777542 1.10807863083966e-33 MirTarget -0.190841372088135 4.05600831845751e-09 NA NA NA hsa-miR-19a-3p CCND2 0.817507952171848 3.76285090469625e-08 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.185131062383478 6.94387115240903e-13 NA NA NA hsa-miR-19b-3p CCND2 0.139124391010031 0.255658553872934 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;MirTarget;miRNATAP -0.166767747836903 1.62862778749919e-07 NA NA NA hsa-miR-200a-3p CCND2 2.55218480823434 2.96135446816238e-51 -1.32381712777542 1.10807863083966e-33 MirTarget -0.261226562912324 4.31939405604759e-36 NA NA NA hsa-miR-20a-5p CCND2 0.44979911120222 0.000602386964327228 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;miRTarBase;miRNATAP -0.235095611327971 1.01223581717651e-15 NA NA NA hsa-miR-20b-5p CCND2 0.78236583455125 8.7938334801223e-05 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.104708964184932 6.77204420924901e-08 NA NA NA hsa-miR-21-3p CCND2 2.17476065946878 5.15299442583591e-75 -1.32381712777542 1.10807863083966e-33 mirMAP -0.127404398971718 2.08059143790106e-05 NA NA NA hsa-miR-29b-3p CCND2 0.438847962603729 6.46722433236121e-05 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.166630108966211 2.5453033344596e-06 22330340;24130168 In addition to CCND2 miR-29 also targets E2F7 another cell cycle regulator;We further demonstrated that miR-29 family acted as tumor suppressors through targeting CCND2 and matrix metalloproteinase-2 genes in GC ; sarcoma;gastric cancer hsa-miR-301a-3p CCND2 1.32170168375632 2.12280368957514e-15 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate -0.182363632693643 1.4299291622836e-15 NA NA NA hsa-miR-3065-3p CCND2 1.62787885002468 6.81477830005026e-19 -1.32381712777542 1.10807863083966e-33 MirTarget;miRNATAP -0.215305118861858 1.68654607913855e-26 NA NA NA hsa-miR-30d-3p CCND2 0.264544633563642 0.00755023189129747 -1.32381712777542 1.10807863083966e-33 mirMAP -0.277305057640272 1.40644960106378e-12 NA NA NA hsa-miR-320b CCND2 0.412787504747938 0.00113457276903985 -1.32381712777542 1.10807863083966e-33 mirMAP;miRNATAP -0.105338094438455 0.000662995774848357 NA NA NA hsa-miR-324-3p CCND2 0.404877731996915 0.000340036423920834 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate -0.187482582074807 4.91214943402943e-08 NA NA NA hsa-miR-331-5p CCND2 0.682437632502949 4.10871642662371e-11 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.224823939820064 1.69348201302645e-09 NA NA NA hsa-miR-342-3p CCND2 1.21944481975657 6.81914373001202e-19 -1.32381712777542 1.10807863083966e-33 miRNAWalker2_validate;mirMAP -0.103848624831325 0.000189231379572542 NA NA NA hsa-miR-429 CCND2 2.84108892259147 3.88520896426515e-64 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.232180070081619 1.04111918722712e-28 NA NA NA hsa-miR-590-3p CCND2 1.27438512890514 1.03164723481469e-23 -1.32381712777542 1.10807863083966e-33 miRanda;mirMAP -0.13833737728579 9.4871454586344e-06 NA NA NA hsa-miR-590-5p CCND2 0.746515607868274 8.51300479640125e-09 -1.32381712777542 1.10807863083966e-33 mirMAP -0.151134124586935 3.93710909950627e-07 NA NA NA hsa-miR-660-5p CCND2 0.406910761707485 0.000156175371762464 -1.32381712777542 1.10807863083966e-33 mirMAP -0.106401121539045 0.00339848496849202 NA NA NA hsa-miR-7-1-3p CCND2 0.686579567507582 1.20731554448806e-07 -1.32381712777542 1.10807863083966e-33 mirMAP -0.138929082076826 3.22847149980611e-06 NA NA NA hsa-miR-93-5p CCND2 1.01788600209314 1.24312921618126e-21 -1.32381712777542 1.10807863083966e-33 miRNATAP -0.400435186075896 5.37578279205249e-31 NA NA NA hsa-miR-96-5p CCND2 3.24596558166606 1.92296159041707e-98 -1.32381712777542 1.10807863083966e-33 TargetScan;miRNATAP -0.311897703713413 7.58506265459077e-49 NA NA NA hsa-miR-125b-5p CCNE1 -1.94393269763542 2.78180543229264e-58 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate -0.382224070637194 4.63212335978261e-14 NA NA NA hsa-miR-195-5p CCNE1 -1.47508108420708 2.20685847368258e-38 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate;MirTarget;miRNATAP -0.617319346276305 3.09483424185554e-30 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.481180084102235 1.82368246174758e-10 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate;miRTarBase;miRNATAP -0.619294201366171 1.19374874415568e-12 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-26b-5p CCNE1 -0.331527896896866 0.000551258576575092 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate;miRTarBase;miRNATAP -0.319970519742405 4.01394740915552e-06 NA NA NA hsa-miR-342-5p CCNE1 1.49554286219821 1.56221300255782e-22 2.02272205094151 6.47439339208807e-27 miRNAWalker2_validate -0.135292830202672 0.00141592689314045 NA NA NA hsa-miR-497-5p CCNE1 -1.4667331575679 5.49909921822638e-36 2.02272205094151 6.47439339208807e-27 MirTarget;miRNATAP -0.382195878498221 1.36469362381851e-12 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-miR-26a-5p CCNE2 -0.481180084102235 1.82368246174758e-10 2.57138773577447 1.5602638336465e-57 miRNAWalker2_validate;miRTarBase;miRNATAP -0.630654803219519 2.81902449356968e-16 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-28-3p CCNE2 -0.815347900986328 3.29387468083828e-30 2.57138773577447 1.5602638336465e-57 PITA;miRNATAP -0.332464341790485 3.74937420435342e-05 NA NA NA hsa-miR-30a-5p CCNE2 -0.392489994825279 0.00954894667761676 2.57138773577447 1.5602638336465e-57 miRNATAP -0.389839068904493 1.17236295202714e-24 NA NA NA hsa-miR-30c-5p CCNE2 -0.393222674580066 8.06961465717306e-05 2.57138773577447 1.5602638336465e-57 miRNATAP -0.253921413883948 1.85400203918647e-05 NA NA NA hsa-miR-335-3p CCNE2 -1.88448538773649 2.09538234398335e-32 2.57138773577447 1.5602638336465e-57 mirMAP -0.16561143561413 3.48668881373491e-06 NA NA NA hsa-miR-34c-5p CCNE2 -0.0896438810345765 0.557016551646841 2.57138773577447 1.5602638336465e-57 miRNAWalker2_validate;miRTarBase;PITA;miRanda;miRNATAP -0.253783325617594 5.10732275535071e-11 NA NA NA hsa-miR-107 CCNG1 0.678998026350662 2.56224148930538e-16 -0.866458495831471 1.01867596168613e-31 miRanda -0.237149829711635 1.74540272426648e-14 NA NA NA hsa-miR-142-5p CCNG1 1.16509303143593 1.96641162209073e-13 -0.866458495831471 1.01867596168613e-31 mirMAP -0.12004392759945 1.00877506047323e-13 NA NA NA hsa-miR-146b-5p CCNG1 0.631041661167954 6.20366593452965e-08 -0.866458495831471 1.01867596168613e-31 miRanda -0.194515367287917 9.40823223569741e-19 NA NA NA hsa-miR-17-3p CCNG1 0.721439431208839 7.95900548819742e-10 -0.866458495831471 1.01867596168613e-31 miRNAWalker2_validate -0.246283992121339 1.69553163929697e-30 NA NA NA hsa-miR-181a-5p CCNG1 0.635863607630183 6.72486918157744e-11 -0.866458495831471 1.01867596168613e-31 miRNAWalker2_validate -0.277078841822535 1.70198624296595e-26 NA NA NA hsa-miR-21-5p CCNG1 2.32007501846389 3.08394781855554e-137 -0.866458495831471 1.01867596168613e-31 miRNAWalker2_validate -0.351124695573807 1.0959074492675e-55 NA NA NA hsa-miR-24-3p CCNG1 -0.120123317289941 0.21432376743243 -0.866458495831471 1.01867596168613e-31 miRNAWalker2_validate -0.135604235725327 6.27170519101967e-07 NA NA NA hsa-miR-27a-3p CCNG1 0.388496361379095 1.42373532086331e-05 -0.866458495831471 1.01867596168613e-31 MirTarget;miRNATAP -0.250909668704439 3.54653910453802e-18 NA NA NA hsa-miR-27b-3p CCNG1 -0.0796803270437447 0.410503155574393 -0.866458495831471 1.01867596168613e-31 MirTarget;miRNATAP -0.120004661367252 1.05794602092694e-05 26623719 Moreover miR-27b directly targets the 3' untranslated regions 3'-UTRs of CCNG1 a well-known negative regulator of P53 stability; Interestingly miR-27b up-regulation leads to increased miR-508-5p expression and this phenomenon is mediated by CCNG1 and P53 gastric cancer hsa-miR-361-5p CCNG1 -0.0996867547900457 0.095428250126326 -0.866458495831471 1.01867596168613e-31 miRanda -0.218834302957743 9.12910136073576e-07 NA NA NA hsa-miR-532-5p CCNG1 0.0386976222860582 0.682018574136515 -0.866458495831471 1.01867596168613e-31 MirTarget -0.253998757759864 1.86433652033132e-20 NA NA NA hsa-miR-590-3p CCNG1 1.27438512890514 1.03164723481469e-23 -0.866458495831471 1.01867596168613e-31 miRanda -0.132032745662621 2.67852852501089e-10 NA NA NA hsa-let-7f-1-3p CCNG2 0.37532726250321 0.00156021688753682 -0.149650768150032 0.140842306998986 MirTarget;mirMAP -0.102144454016075 0.000581812932406976 NA NA NA hsa-miR-106b-5p CCNG2 1.22477789913175 2.31787213143326e-32 -0.149650768150032 0.140842306998986 MirTarget;miRNATAP -0.306458644229248 3.58888543190336e-22 NA NA NA hsa-miR-142-5p CCNG2 1.16509303143593 1.96641162209073e-13 -0.149650768150032 0.140842306998986 MirTarget;miRNATAP -0.128046224919324 2.42603211295912e-09 NA NA NA hsa-miR-146b-5p CCNG2 0.631041661167954 6.20366593452965e-08 -0.149650768150032 0.140842306998986 miRNATAP -0.223398301619967 2.26365927600617e-14 NA NA NA hsa-miR-16-1-3p CCNG2 1.30142784811196 2.53343713753744e-29 -0.149650768150032 0.140842306998986 MirTarget -0.15931215973936 1.98536912855598e-07 NA NA NA hsa-miR-16-2-3p CCNG2 0.286355254959005 0.0256946046884474 -0.149650768150032 0.140842306998986 mirMAP -0.197193636377051 2.55642252045045e-13 NA NA NA hsa-miR-17-5p CCNG2 0.599531757298529 1.07318660415906e-05 -0.149650768150032 0.140842306998986 MirTarget;TargetScan;miRNATAP -0.259776502216066 7.42418835624223e-26 NA NA NA hsa-miR-181a-5p CCNG2 0.635863607630183 6.72486918157744e-11 -0.149650768150032 0.140842306998986 mirMAP -0.150706985857032 2.03465396573207e-05 NA NA NA hsa-miR-181b-5p CCNG2 1.22749257795094 1.21998198830919e-27 -0.149650768150032 0.140842306998986 mirMAP -0.175819136208059 3.26451277799745e-09 NA NA NA hsa-miR-20a-5p CCNG2 0.44979911120222 0.000602386964327228 -0.149650768150032 0.140842306998986 MirTarget;miRNATAP -0.255617165198528 4.22661406827169e-23 NA NA NA hsa-miR-21-5p CCNG2 2.32007501846389 3.08394781855554e-137 -0.149650768150032 0.140842306998986 mirMAP -0.100576766109129 0.00147226728207765 NA NA NA hsa-miR-224-5p CCNG2 -2.22249420723892 1.07787949391113e-18 -0.149650768150032 0.140842306998986 mirMAP -0.124461718628112 3.77609224120173e-21 NA NA NA hsa-miR-28-5p CCNG2 -0.14267073663385 0.0497619246260571 -0.149650768150032 0.140842306998986 miRanda -0.202065115294172 2.7892462854838e-05 NA NA NA hsa-miR-29a-5p CCNG2 0.334183773952055 0.00445137265870197 -0.149650768150032 0.140842306998986 mirMAP -0.132668181770376 7.50885653033592e-06 NA NA NA hsa-miR-340-5p CCNG2 1.0857159627596 4.94471062546756e-24 -0.149650768150032 0.140842306998986 MirTarget;mirMAP -0.15902579298036 4.25556894816954e-07 27374211 MicroRNA 340 promotes the tumor growth of human gastric cancer by inhibiting cyclin G2; Notably alteration of miR-340 expression affected the luciferase activity of wild-type 3'-UTR of cyclin G2 CCNG2 and regulated CCNG2 abundance in gastric cancer cells indicating that CCNG2 is a direct target of miR-340; Moreover CCNG2 knockdown eradicated the effects of miR-340 silencing on gastric cancer cells gastric cancer hsa-miR-374a-5p CCNG2 -0.239709888246804 0.00309819667153627 -0.149650768150032 0.140842306998986 mirMAP -0.136234432245765 0.00160962126046218 NA NA NA hsa-miR-374b-5p CCNG2 -0.466781429946153 1.17238311619116e-08 -0.149650768150032 0.140842306998986 mirMAP -0.160598081573648 0.000152391168246208 NA NA NA hsa-miR-576-5p CCNG2 0.0649387707567879 0.588791966096287 -0.149650768150032 0.140842306998986 mirMAP -0.135613484469682 2.78689779009329e-06 NA NA NA hsa-miR-590-3p CCNG2 1.27438512890514 1.03164723481469e-23 -0.149650768150032 0.140842306998986 miRanda;mirMAP -0.218641605173616 1.66036386703255e-15 NA NA NA hsa-miR-590-5p CCNG2 0.746515607868274 8.51300479640125e-09 -0.149650768150032 0.140842306998986 mirMAP -0.237225390915899 9.58760682122434e-20 NA NA NA hsa-miR-708-3p CCNG2 0.478640655706813 2.13865852505288e-05 -0.149650768150032 0.140842306998986 MirTarget -0.124006998567315 5.60282600425757e-05 NA NA NA hsa-miR-93-5p CCNG2 1.01788600209314 1.24312921618126e-21 -0.149650768150032 0.140842306998986 miRNAWalker2_validate;MirTarget;miRNATAP -0.303642592411967 1.82602983231745e-22 NA NA NA hsa-miR-125a-3p CD82 -0.176412960953959 0.10108079286419 -0.203742156546495 0.118832889927554 miRanda -0.126418775900477 0.00250164869889348 NA NA NA hsa-miR-10b-3p CDK2 -1.58463104907636 1.17335119858099e-46 0.628977809366634 3.17063810755044e-21 mirMAP -0.210836970989172 1.24690584925124e-27 NA NA NA hsa-miR-10b-5p CDK2 -2.10996264627796 7.36811595736367e-72 0.628977809366634 3.17063810755044e-21 miRNAWalker2_validate -0.172779155677722 1.52256449776603e-22 NA NA NA hsa-miR-145-5p CDK4 -2.62703571936687 7.64402900188551e-88 0.597969315491349 4.23701383421285e-22 miRNAWalker2_validate;miRTarBase -0.134364969525933 9.65405723067241e-21 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-195-5p CDK4 -1.47508108420708 2.20685847368258e-38 0.597969315491349 4.23701383421285e-22 miRNAWalker2_validate;miRTarBase -0.168636406260907 2.79019414251395e-21 NA NA NA hsa-miR-103a-3p CDK6 0.610673390034345 1.63113920514646e-12 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRNATAP -0.41897711298053 2.13541829274935e-12 NA NA NA hsa-miR-107 CDK6 0.678998026350662 2.56224148930538e-16 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRTarBase;PITA;miRNATAP -0.220761526250824 0.000423296532800194 19407485;22491216;21264532;19688090 Enforced expression of miR-107 in MiaPACA-2 and PANC-1 cells downregulated in vitro growth and this was associated with repression of the putative miR-107 target cyclin-dependent kinase 6 thereby providing a functional basis for the epigenetic inactivation of this miRNA in pancreatic cancer;Levels of known miR-107 targets protein kinase Cε PKCε cyclin-dependent kinase 6 CDK6 and hypoxia-inducible factor 1-β HIF1-β decreased following NP/pre-miR-107 treatment;We have identified miR-107 as a potential regulator of CDK6 expression; A bioinformatics search revealed a putative target site for miR-107 within the CDK6 3' untranslated region; Expression of miR-107 in gastric cancer cell lines was found inversely correlated with CDK6 expression; miR-107 could significantly suppress CDK6 3' UTR luciferase reporter activity and this effect was not detectable when the putative 3' UTR target site was mutated; Consistent with the results of the reporter assay ectopic expression of miR-107 reduced both mRNA and protein expression levels of CDK6 inhibited proliferation induced G1 cell cycle arrest and blocked invasion of the gastric cancer cells; Our results suggest that miR-107 may have a tumor suppressor function by directly targeting CDK6 to inhibit the proliferation and invasion activities of gastric cancer cells;Using miRNA-target prediction analyses and the array data we listed up a set of likely targets of miR-107 and miR-185 for G1 cell cycle arrest and validate a subset of them using real-time RT-PCR and immunoblotting for CDK6 ;;; pancreatic cancer;head and neck cancer;gastric cancer;lung squamous cell cancer hsa-miR-141-3p CDK6 2.99777540204168 1.26370498493024e-78 -1.03099881099472 9.88361268912926e-12 TargetScan;miRNATAP -0.155074529575108 1.68227129058067e-07 NA NA NA hsa-miR-15a-5p CDK6 0.812445324157528 4.50263417099822e-20 -1.03099881099472 9.88361268912926e-12 miRNATAP -0.307441214732404 1.1453244726092e-07 NA NA NA hsa-miR-182-5p CDK6 2.36408084103818 2.60011457600281e-72 -1.03099881099472 9.88361268912926e-12 mirMAP -0.302395516858316 3.47976531991082e-17 NA NA NA hsa-miR-191-5p CDK6 0.831652724468215 1.98847993606895e-12 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRTarBase -0.206787526199797 2.32979427977823e-06 NA NA NA hsa-miR-193b-3p CDK6 -0.0550374855074773 0.65382485898551 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate -0.155046597813103 0.00030084184892796 NA NA NA hsa-miR-200a-3p CDK6 2.55218480823434 2.96135446816238e-51 -1.03099881099472 9.88361268912926e-12 miRNATAP -0.153245463849065 1.36473748839668e-07 24009066 microRNA 200a is an independent prognostic factor of hepatocellular carcinoma and induces cell cycle arrest by targeting CDK6 liver cancer hsa-miR-200b-3p CDK6 2.11744072703209 1.66710572104191e-43 -1.03099881099472 9.88361268912926e-12 mirMAP -0.174815127052701 6.39294256795547e-08 NA NA NA hsa-miR-200c-3p CDK6 2.07038651806183 6.84613386286599e-51 -1.03099881099472 9.88361268912926e-12 mirMAP -0.180928310145507 3.68922155138771e-07 NA NA NA hsa-miR-21-5p CDK6 2.32007501846389 3.08394781855554e-137 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;mirMAP -0.197449989063307 3.4492907261557e-05 NA NA NA hsa-miR-29c-3p CDK6 -0.0652732378039769 0.563774115756922 -1.03099881099472 9.88361268912926e-12 miRNAWalker2_validate;miRTarBase;miRNATAP -0.390844229843503 1.3743800981758e-17 26396669 Furthermore through qPCR and Western blot assays confirmed that overexpression of miR-29c reduced CDK6 mRNA and protein levels; miR-29c could inhibit the proliferation migration and invasion of bladder cancer cells via regulating CDK6 bladder cancer hsa-miR-30a-5p CDK6 -0.392489994825279 0.00954894667761676 -1.03099881099472 9.88361268912926e-12 mirMAP -0.124807010230796 0.000314782970367504 NA NA NA hsa-miR-338-3p CDK6 0.0799903526884966 0.575137668326679 -1.03099881099472 9.88361268912926e-12 mirMAP -0.191800207428879 1.71408750980913e-07 NA NA NA hsa-miR-425-5p CDK6 0.719898949241718 6.01079610190374e-08 -1.03099881099472 9.88361268912926e-12 mirMAP -0.150921506027425 0.000119900868002377 NA NA NA hsa-miR-429 CDK6 2.84108892259147 3.88520896426515e-64 -1.03099881099472 9.88361268912926e-12 mirMAP;miRNATAP -0.133862145668 3.72317905758963e-06 NA NA NA hsa-miR-664a-3p CDK6 -0.237699857923843 0.0197071390183375 -1.03099881099472 9.88361268912926e-12 mirMAP -0.195343666542438 0.000155666654807136 NA NA NA hsa-miR-92b-3p CDK6 1.30248132773644 9.42428674470985e-25 -1.03099881099472 9.88361268912926e-12 miRNATAP -0.108885215584239 0.00691585930585228 NA NA NA hsa-let-7d-5p CDKN1A 0.410958462607207 6.07808182756787e-06 -0.254319078213554 0.025069242968496 MirTarget -0.209534907854426 9.05682787226141e-07 NA NA NA hsa-let-7g-5p CDKN1A 0.0200025784917734 0.791635298815256 -0.254319078213554 0.025069242968496 MirTarget -0.234754189393088 5.7315667018148e-06 NA NA NA hsa-miR-101-3p CDKN1A -0.126035286271986 0.145997832808138 -0.254319078213554 0.025069242968496 MirTarget -0.12547684489979 0.00551360058669363 NA NA NA hsa-miR-106a-5p CDKN1A 0.574594373206545 0.000145868284001732 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.114530864663224 7.53068059714178e-06 25510666 After prediction with online software we further used dual-luciferase reporter gene assay to ensure that TP53INP1 and CDKN1A might be the direct targets of miR-106a liver cancer hsa-miR-106b-5p CDKN1A 1.22477789913175 2.31787213143326e-32 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.198769219405748 3.53449473460544e-08 NA NA NA hsa-miR-146a-5p CDKN1A 0.102405752365098 0.486975378688068 -0.254319078213554 0.025069242968496 miRNAWalker2_validate -0.112996377410099 1.83135810400461e-05 NA NA NA hsa-miR-17-5p CDKN1A 0.599531757298529 1.07318660415906e-05 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.157707858341151 2.444032078598e-08 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-182-5p CDKN1A 2.36408084103818 2.60011457600281e-72 -0.254319078213554 0.025069242968496 miRNAWalker2_validate -0.114208671947908 2.29748891177513e-05 NA NA NA hsa-miR-20a-5p CDKN1A 0.44979911120222 0.000602386964327228 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.126075559540983 1.99860276007902e-05 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-28-5p CDKN1A -0.14267073663385 0.0497619246260571 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRanda;miRNATAP -0.294350864895331 4.29596264827348e-08 NA NA NA hsa-miR-423-3p CDKN1A 0.338351552541003 0.000947983901628776 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase -0.105368362155166 0.00567064832994105 NA NA NA hsa-miR-455-3p CDKN1A 0.678392920628872 0.000327758844555877 -0.254319078213554 0.025069242968496 MirTarget -0.119773167671374 4.14708070797368e-09 NA NA NA hsa-miR-505-5p CDKN1A -0.842640534241897 4.43022473091907e-09 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;MirTarget -0.108248027724388 5.69419612511767e-05 NA NA NA hsa-miR-93-5p CDKN1A 1.01788600209314 1.24312921618126e-21 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.147676795890733 3.39439379040982e-05 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 0.0274758353801279 0.842368857038259 -0.254319078213554 0.025069242968496 miRNAWalker2_validate -0.114405823345184 5.05622411237802e-05 NA NA NA hsa-miR-96-5p CDKN1A 3.24596558166606 1.92296159041707e-98 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;miRTarBase -0.102209126041297 4.86561723152027e-06 26582573 Upregulation of microRNA 96 and its oncogenic functions by targeting CDKN1A in bladder cancer; Bioinformatics prediction combined with luciferase reporter assay were used to verify whether the cyclin-dependent kinase inhibitor CDKN1A was a potential target gene of miR-96; According to the data of miRTarBase CDKN1A might be a candidate target gene of miR-96; In addition luciferase reporter and Western blot assays respectively demonstrated that miR-96 could bind to the putative seed region in CDKN1A mRNA 3'UTR and significantly reduce the expression level of CDKN1A protein; Moreover we found that the inhibition of miR-96 expression remarkably decreased cell proliferation and promoted cell apoptosis of BC cell lines which was consistent with the findings observed following the introduction of CDKN1A cDNA without 3'UTR restored miR-96; Upregulation of miR-96 may contribute to aggressive malignancy partly through suppressing CDKN1A protein expression in BC cells bladder cancer hsa-miR-98-5p CDKN1A 0.656336869298973 9.03113188590466e-15 -0.254319078213554 0.025069242968496 miRNAWalker2_validate;MirTarget -0.175027497589803 0.000116761897589387 NA NA NA hsa-miR-10b-5p CDKN2A -2.10996264627796 7.36811595736367e-72 2.12039399576679 1.36950972049671e-25 miRNAWalker2_validate;miRTarBase -0.715222702174389 1.70829699904957e-41 NA NA NA hsa-miR-125a-5p CDKN2A -0.635694700253268 3.6703235721638e-11 2.12039399576679 1.36950972049671e-25 miRanda -0.588080813765285 7.0950686216042e-16 NA NA NA hsa-miR-125b-5p CDKN2A -1.94393269763542 2.78180543229264e-58 2.12039399576679 1.36950972049671e-25 miRNAWalker2_validate -0.436831582226038 9.31110596805625e-16 23585871 In this study we further extend our studies by showing that miR-125b represses the protein product of the ink4a/ARF locus p14ARF in two prostate cancer cell lines LNCaP wild type-p53 and 22Rv1 both wild type and mutant p53 as well as in the PC-346C prostate cancer xenograft model that lentivirally overexpressed miR-125b; Conversely treatment of prostate cancer cells with an inhibitor of miR-125b anti-miR-125b resulted in increased expression of p14ARF decreased level of Mdm2 and induction of apoptosis; In addition overexpression of miR-125b in p53-deficient PC3 cells induced down-regulation of p14ARF which leads to increased cell proliferation through a p53-independent manner prostate cancer hsa-miR-365a-3p CDKN2A -1.45032725192851 2.37888098061118e-37 2.12039399576679 1.36950972049671e-25 MirTarget -0.389403479218473 7.60310258658509e-11 NA NA NA hsa-miR-139-5p CHEK1 -3.25846663863766 2.62018989415003e-112 1.25798968980226 1.05480144252256e-29 miRanda -0.300486863727614 2.11897109250447e-40 NA NA NA hsa-miR-195-5p CHEK1 -1.47508108420708 2.20685847368258e-38 1.25798968980226 1.05480144252256e-29 MirTarget;miRNATAP -0.385549885297148 9.47498908417406e-34 25840419 MiR 195 suppresses non small cell lung cancer by targeting CHEK1; We discovered that CHEK1 was a direct target of miR-195 which decreased CHEK1 expression in lung cancer cells lung squamous cell cancer hsa-miR-495-3p CHEK1 -1.67685437612894 1.70634429769052e-34 1.25798968980226 1.05480144252256e-29 MirTarget -0.163938153068567 2.61716526324285e-09 NA NA NA hsa-miR-497-5p CHEK1 -1.4667331575679 5.49909921822638e-36 1.25798968980226 1.05480144252256e-29 MirTarget;miRNATAP -0.193287579748198 1.64610972227681e-09 24464213 Checkpoint kinase 1 is negatively regulated by miR 497 in hepatocellular carcinoma; In silico analysis showed that CHEK1 was a candidate target of miR-497 which was previously found to be downregulated in HCC by us; To test whether miR-497 could bind to 3'untranslated region 3'UTR of CHEK1 luciferase reporter assay was conducted; The result revealed that miR-497 could bind to the 3'untranslated region 3'UTR of CHEK1 mRNA; Western blot showed that ectopic expression of miR-497 suppressed the CHEK1 expression and inhibition of miR-497 led to significant upregulation of CHEK1; Finally miR-497 expression was measured in the same 30 HCC samples and the correlation between miR-497 and CHEK1 was analyzed; The results indicated that miR-497 was downregulated in HCC and had a significant negative correlation with CHEK1; Taken together these results demonstrated that CHEK1 was negatively regulated by miR-497 and the overexpressed CHEK1 was resulted from the downregulated miR-497 in HCC which provided a potential molecular target for HCC therapy liver cancer hsa-miR-139-5p CYCS -3.25846663863766 2.62018989415003e-112 0.514544707933127 7.20434543139638e-13 miRanda -0.138771276194956 1.91475094017139e-21 NA NA NA hsa-miR-34c-5p CYCS -0.0896438810345765 0.557016551646841 0.514544707933127 7.20434543139638e-13 miRanda -0.106641084379626 4.91599767479111e-11 NA NA NA hsa-miR-495-3p CYCS -1.67685437612894 1.70634429769052e-34 0.514544707933127 7.20434543139638e-13 mirMAP -0.103209359396361 2.73071281717473e-09 NA NA NA hsa-let-7a-5p FAS -0.210286642041636 0.0072546234707717 -0.759637724162876 2.18688041634812e-12 TargetScan -0.147010045095826 0.00234531576557678 NA NA NA hsa-miR-338-3p FAS 0.0799903526884966 0.575137668326679 -0.759637724162876 2.18688041634812e-12 miRanda -0.16768795837118 1.3473835302647e-10 NA NA NA hsa-miR-98-5p FAS 0.656336869298973 9.03113188590466e-15 -0.759637724162876 2.18688041634812e-12 miRNAWalker2_validate -0.147800482737569 0.000760862411963529 NA NA NA hsa-miR-148b-3p GADD45A 1.0552576751879 1.6679285705231e-29 -0.208892067351644 0.0348101740051854 MirTarget -0.231496451348305 2.77073546077852e-11 NA NA NA hsa-miR-331-5p GADD45A 0.682437632502949 4.10871642662371e-11 -0.208892067351644 0.0348101740051854 PITA;miRNATAP -0.123517623290777 0.000150251086827537 NA NA NA hsa-miR-1976 GADD45B 0.289190368864725 0.00167092758216584 0.00189543029343842 0.986264142476403 miRNATAP -0.184077522593828 6.9983734252811e-06 NA NA NA hsa-miR-324-3p GADD45B 0.404877731996915 0.000340036423920834 0.00189543029343842 0.986264142476403 MirTarget;miRNATAP -0.272759607730261 8.22009676059255e-17 NA NA NA hsa-miR-590-3p GADD45B 1.27438512890514 1.03164723481469e-23 0.00189543029343842 0.986264142476403 miRanda -0.144434950089762 1.57603896644092e-06 NA NA NA hsa-miR-1468-5p GADD45G -1.39112473580933 7.93337422009489e-22 1.62405350947909 6.25407490128674e-20 MirTarget -0.329853422258391 1.46530042095361e-15 NA NA NA hsa-miR-1976 GADD45G 0.289190368864725 0.00167092758216584 1.62405350947909 6.25407490128674e-20 MirTarget -0.31549221771233 3.22063864824767e-06 NA NA NA hsa-miR-139-5p GTSE1 -3.25846663863766 2.62018989415003e-112 3.15237963595094 3.60980225897754e-81 miRanda -0.592741491633758 3.08579032878793e-65 NA NA NA hsa-miR-491-5p GTSE1 -1.04307210952796 1.14586501470398e-15 3.15237963595094 3.60980225897754e-81 miRanda -0.287912561136366 1.19062679883994e-09 NA NA NA hsa-let-7a-3p IGF1 0.466622567891891 3.70886257943079e-07 -2.74966407318656 1.16668946763881e-41 mirMAP -0.598494517106358 2.99480834420479e-14 NA NA NA hsa-let-7f-1-3p IGF1 0.37532726250321 0.00156021688753682 -2.74966407318656 1.16668946763881e-41 mirMAP -0.284984074850365 5.12019686555637e-06 NA NA NA hsa-miR-130b-3p IGF1 1.38122163823846 1.00932179737836e-26 -2.74966407318656 1.16668946763881e-41 MirTarget -0.632738321082739 2.08097755219068e-32 NA NA NA hsa-miR-142-3p IGF1 1.53951237846104 2.06769936282034e-17 -2.74966407318656 1.16668946763881e-41 miRanda -0.123324920262687 0.00187203848886841 NA NA NA hsa-miR-148a-5p IGF1 0.572692271754695 2.14306251522796e-06 -2.74966407318656 1.16668946763881e-41 mirMAP -0.165502731566367 0.00629257314556201 NA NA NA hsa-miR-155-5p IGF1 1.09766314984151 4.55650930885814e-15 -2.74966407318656 1.16668946763881e-41 mirMAP -0.165951614876576 0.00129161967089585 NA NA NA hsa-miR-15b-3p IGF1 1.12898579025388 1.18050912917317e-14 -2.74966407318656 1.16668946763881e-41 mirMAP -0.538169881674298 1.72833038996145e-29 NA NA NA hsa-miR-16-1-3p IGF1 1.30142784811196 2.53343713753744e-29 -2.74966407318656 1.16668946763881e-41 mirMAP -0.666577091007243 4.14343498994569e-26 NA NA NA hsa-miR-181a-5p IGF1 0.635863607630183 6.72486918157744e-11 -2.74966407318656 1.16668946763881e-41 mirMAP -0.494938478163062 2.34360673909487e-11 NA NA NA hsa-miR-181b-5p IGF1 1.22749257795094 1.21998198830919e-27 -2.74966407318656 1.16668946763881e-41 mirMAP -0.585684202347318 2.25280244287885e-21 NA NA NA hsa-miR-181c-5p IGF1 0.574847839298605 4.51287502066478e-07 -2.74966407318656 1.16668946763881e-41 mirMAP -0.395598534798654 5.11211832683976e-10 NA NA NA hsa-miR-186-5p IGF1 -0.426987301460247 1.04129380549315e-05 -2.74966407318656 1.16668946763881e-41 mirMAP -0.219700941598902 0.00375910687826014 NA NA NA hsa-miR-19a-3p IGF1 0.817507952171848 3.76285090469625e-08 -2.74966407318656 1.16668946763881e-41 MirTarget -0.513925086378424 2.94832706936413e-27 NA NA NA hsa-miR-19b-1-5p IGF1 0.647704962003062 3.07687667945597e-07 -2.74966407318656 1.16668946763881e-41 mirMAP -0.553744322104364 7.97205759882393e-23 NA NA NA hsa-miR-19b-3p IGF1 0.139124391010031 0.255658553872934 -2.74966407318656 1.16668946763881e-41 MirTarget -0.446691776662058 5.00619914718767e-14 NA NA NA hsa-miR-20a-3p IGF1 -0.218902047543766 0.179868363625547 -2.74966407318656 1.16668946763881e-41 mirMAP -0.256736318876148 1.0275917705269e-08 NA NA NA hsa-miR-27a-3p IGF1 0.388496361379095 1.42373532086331e-05 -2.74966407318656 1.16668946763881e-41 miRNAWalker2_validate;miRTarBase -0.544265988607736 2.14223289540701e-11 NA NA NA hsa-miR-29b-3p IGF1 0.438847962603729 6.46722433236121e-05 -2.74966407318656 1.16668946763881e-41 MirTarget -0.414656321477745 3.77745108303762e-10 25592039 Luciferase reporter assays were conducted to determine the association between miR-29b and the insulin-like growth factor 1 IGF1 3' untranslated region 3'UTR; IGF1 an activator of PI3K/Akt signaling was confirmed as a novel target of miR-29b colorectal cancer hsa-miR-301a-3p IGF1 1.32170168375632 2.12280368957514e-15 -2.74966407318656 1.16668946763881e-41 MirTarget -0.553869611428521 1.38070780054886e-40 NA NA NA hsa-miR-320b IGF1 0.412787504747938 0.00113457276903985 -2.74966407318656 1.16668946763881e-41 miRNATAP -0.353937956338846 8.47295336022305e-10 NA NA NA hsa-miR-362-5p IGF1 -0.248676340901797 0.0726089851456282 -2.74966407318656 1.16668946763881e-41 mirMAP -0.161368577599581 0.00235431302635106 NA NA NA hsa-miR-3913-5p IGF1 0.71890413546147 2.61153227160465e-13 -2.74966407318656 1.16668946763881e-41 mirMAP -0.344116396893892 3.11162487969542e-06 NA NA NA hsa-miR-454-3p IGF1 1.28177672191095 6.84024979484587e-21 -2.74966407318656 1.16668946763881e-41 MirTarget -0.592555267211157 2.36575948576207e-31 NA NA NA hsa-miR-576-5p IGF1 0.0649387707567879 0.588791966096287 -2.74966407318656 1.16668946763881e-41 PITA;mirMAP;miRNATAP -0.338610461178183 2.7172717713968e-08 NA NA NA hsa-miR-590-3p IGF1 1.27438512890514 1.03164723481469e-23 -2.74966407318656 1.16668946763881e-41 MirTarget;miRanda;mirMAP;miRNATAP -0.58985675364928 6.40681378251621e-25 NA NA NA hsa-miR-629-5p IGF1 0.42411327890895 2.46262300605706e-05 -2.74966407318656 1.16668946763881e-41 mirMAP -0.48961361687082 1.21612287765371e-11 NA NA NA hsa-let-7a-3p IGFBP3 0.466622567891891 3.70886257943079e-07 -0.740251117224754 7.69399599791541e-12 miRNATAP -0.107092706207436 0.00877058958447313 NA NA NA hsa-miR-375 IGFBP3 2.09893673049766 1.68351780396203e-16 -0.740251117224754 7.69399599791541e-12 miRNAWalker2_validate -0.125007540214907 9.99564454514586e-19 NA NA NA hsa-miR-143-3p MDM2 -1.23248181631499 4.74160976678504e-28 0.134052426741699 0.110706751806459 miRNAWalker2_validate -0.116551192492011 2.45785814867103e-06 NA NA NA hsa-miR-181a-2-3p MDM2 -0.0718643470098321 0.422077954889554 0.134052426741699 0.110706751806459 mirMAP -0.12266836491989 0.000146166192040392 NA NA NA hsa-miR-25-3p MDM2 -0.00546790775985073 0.944061771389468 0.134052426741699 0.110706751806459 miRNAWalker2_validate;miRTarBase -0.121996633743668 0.00105879152857406 NA NA NA hsa-miR-340-5p MDM2 1.0857159627596 4.94471062546756e-24 0.134052426741699 0.110706751806459 mirMAP -0.114356673254404 1.13062337969042e-05 26718483 MicroRNA 340 inhibits prostate cancer cell proliferation and metastasis by targeting the MDM2 p53 pathway; Our findings suggest that miR-340 may function as a novel tumor suppressor in PCa through the MDM2-p53 pathway by directly targeting MDM2 which may be a promising miRNA-targeted therapy for PCa metastasis prostate cancer hsa-miR-369-3p MDM2 0.0880848299432269 0.48519534195924 0.134052426741699 0.110706751806459 mirMAP -0.139668513237869 8.70962517671477e-10 NA NA NA hsa-miR-374a-5p MDM2 -0.239709888246804 0.00309819667153627 0.134052426741699 0.110706751806459 mirMAP -0.181528999029164 3.25892646409229e-07 NA NA NA hsa-miR-374b-5p MDM2 -0.466781429946153 1.17238311619116e-08 0.134052426741699 0.110706751806459 mirMAP -0.113653249109849 0.00120350297983546 NA NA NA hsa-miR-485-3p MDM2 -0.866822427729628 4.5194665263492e-11 0.134052426741699 0.110706751806459 mirMAP -0.105743116773406 1.17706611197258e-06 NA NA NA hsa-miR-500a-5p MDM2 0.209805801129289 0.119177819862398 0.134052426741699 0.110706751806459 mirMAP -0.119455592892776 2.98058844851876e-08 NA NA NA hsa-miR-584-5p MDM2 -1.75458362433615 2.80754771470968e-33 0.134052426741699 0.110706751806459 mirMAP -0.102042062933 5.80205890285492e-08 NA NA NA hsa-let-7i-5p MDM4 -0.333419326258177 3.56960810786801e-08 0.113166606771184 0.0960960367986367 MirTarget -0.222294758545999 7.66575137899016e-09 NA NA NA hsa-miR-152-3p MDM4 -0.26603867075042 0.0063861560676033 0.113166606771184 0.0960960367986367 MirTarget -0.125663304235708 1.27968538819022e-07 NA NA NA hsa-miR-27a-3p MDM4 0.388496361379095 1.42373532086331e-05 0.113166606771184 0.0960960367986367 miRNATAP -0.131895989576392 3.49986146304971e-07 NA NA NA hsa-miR-374a-3p MDM4 0.34122393953022 2.28714105382856e-05 0.113166606771184 0.0960960367986367 mirMAP -0.156782565277081 5.20014091932367e-08 NA NA NA hsa-miR-374b-5p MDM4 -0.466781429946153 1.17238311619116e-08 0.113166606771184 0.0960960367986367 mirMAP -0.106203351804964 0.000179652019538332 NA NA NA hsa-miR-125b-5p PMAIP1 -1.94393269763542 2.78180543229264e-58 1.5190355795424 7.37304528466062e-22 miRNAWalker2_validate -0.24538372765234 8.11152959377607e-09 NA NA NA hsa-miR-140-5p PMAIP1 -0.755971041589807 9.68060922934542e-14 1.5190355795424 7.37304528466062e-22 miRanda -0.19401318752549 0.000334650587616517 NA NA NA hsa-miR-22-3p PMAIP1 -0.505819481364718 1.90494905330622e-13 1.5190355795424 7.37304528466062e-22 MirTarget -0.551091337032684 4.69078553444161e-12 NA NA NA hsa-miR-370-3p PMAIP1 -0.678485107633497 3.08223718122071e-07 1.5190355795424 7.37304528466062e-22 MirTarget -0.179221538216079 1.80406422048623e-05 NA NA NA hsa-miR-374a-5p PMAIP1 -0.239709888246804 0.00309819667153627 1.5190355795424 7.37304528466062e-22 mirMAP -0.277766997446705 5.55206844438065e-05 NA NA NA hsa-miR-374b-5p PMAIP1 -0.466781429946153 1.17238311619116e-08 1.5190355795424 7.37304528466062e-22 mirMAP -0.304127838737985 6.96208470768494e-06 NA NA NA hsa-miR-15a-5p PPM1D 0.812445324157528 4.50263417099822e-20 -0.504477915463154 6.8851005612732e-09 MirTarget;miRNATAP -0.270453106366884 1.64543789920528e-16 NA NA NA hsa-miR-29a-3p PPM1D -0.699539666833076 1.13935003139957e-13 -0.504477915463154 6.8851005612732e-09 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.181127531473555 7.76015251016572e-09 NA NA NA hsa-miR-29c-3p PPM1D -0.0652732378039769 0.563774115756922 -0.504477915463154 6.8851005612732e-09 miRNAWalker2_validate;MirTarget;miRNATAP -0.101485696747801 0.000140367625061134 25888625 A suppressive role of ionizing radiation responsive miR 29c in the development of liver carcinoma via targeting WIP1; miR-29c expression is downregulated in human hepatocellular carcinoma cells which is inversely correlated with WIP1 expression; miR-29c attenuates luciferase activity of a reporter harboring the 3'UTR binding motif of WIP1 mRNA; The biological effects of miR-29c may be mediated by its target WIP1 which regulates p53 activity via dephosphorylation at Ser-15; Finally fluorescence in situ hybridization FISH and immunohistochemical analyses indicate that miR-29c is downregulated in 50.6% of liver carcinoma tissues examined whereas WIP1 is upregulated in 45.4% of these tissues; The expression of miR-29c inversely correlates with that of WIP1 in HCC; Our results suggest that the IR-responsive miR-29c may function as a tumor suppressor that plays a crucial role in the development of liver carcinoma via targeting WIP1 therefore possibly representing a target molecule for therapeutic intervention for this disease liver cancer hsa-miR-361-5p PPM1D -0.0996867547900457 0.095428250126326 -0.504477915463154 6.8851005612732e-09 miRanda;miRNATAP -0.230046711955104 6.49792801623323e-06 NA NA NA hsa-miR-103a-3p PTEN 0.610673390034345 1.63113920514646e-12 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase -0.310265672952247 8.75438711531989e-26 26511107;24828205 LncRNA GAS5 induces PTEN expression through inhibiting miR 103 in endometrial cancer cells; To investigate the expression of GAS5 PTEN and miR-103 RT-PCR was performed; Finally we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells; Through inhibiting the expression of miR-103 GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis and thus could be an important mediator in the pathogenesis of endometrial cancer;Our data collectively demonstrate that miR-103 is an oncogene miRNA that promotes colorectal cancer proliferation and migration through down-regulation of the tumor suppressor genes DICER and PTEN ; endometrial cancer;colorectal cancer hsa-miR-106b-5p PTEN 1.22477789913175 2.31787213143326e-32 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.275865014410931 5.66481319436072e-31 26722252;24842611;26238857 Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells;MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance ;cell migration;drug resistance breast cancer;gastric cancer;colorectal cancer hsa-miR-107 PTEN 0.678998026350662 2.56224148930538e-16 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;PITA;miRanda -0.294379341856167 1.4825572602859e-21 NA NA NA hsa-miR-130b-3p PTEN 1.38122163823846 1.00932179737836e-26 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.10664357535475 8.50670598014416e-08 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-132-3p PTEN -0.0365213889238607 0.668598301088667 -0.737945156702629 1.26474514880987e-22 miRNATAP -0.136713479453887 1.19438197426319e-05 NA NA NA hsa-miR-141-3p PTEN 2.99777540204168 1.26370498493024e-78 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.12717832397844 6.47904977288109e-18 27644195;24742567 Involvement of microRNA 141 3p in 5 fluorouracil and oxaliplatin chemo resistance in esophageal cancer cells via regulation of PTEN; Western blot exhibited altered protein levels of PTEN Akt and PI3k with miR-141-3p inhibitor; An inverse correlation between PTEN expression and miR-141-3p expression was also observed in tissue samples; Our study demonstrated that miR-141-3p contributed to an acquired chemo-resistance through PTEN modulation both in vitro and in vivo;PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis drug resistance;tumorigenesis esophageal cancer;endometrial cancer hsa-miR-142-5p PTEN 1.16509303143593 1.96641162209073e-13 -0.737945156702629 1.26474514880987e-22 MirTarget;PITA -0.109721832085825 1.70710191686431e-11 NA NA NA hsa-miR-146b-5p PTEN 0.631041661167954 6.20366593452965e-08 -0.737945156702629 1.26474514880987e-22 miRanda -0.188813122906075 1.83574416282237e-17 NA NA NA hsa-miR-16-1-3p PTEN 1.30142784811196 2.53343713753744e-29 -0.737945156702629 1.26474514880987e-22 MirTarget -0.13766907248918 3.37650605787999e-09 NA NA NA hsa-miR-17-5p PTEN 0.599531757298529 1.07318660415906e-05 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;TargetScan;miRNATAP -0.152588360384734 1.28038204158757e-15 26629823;27400681;23391506;23133552;24462867;26318586;26215320;25634356;26500892;24912422;23418359 We hypothesized that knocking down the oncogenic microRNA oncomiR miR-17-5p might restore the expression levels of PDCD4 and PTEN tumor suppressor proteins illustrating a route to oligonucleotide therapy of TNBC; Contrary to conventional wisdom antisense knockdown of oncomiR miR-17-5p guide strand reduced PDCD4 and PTEN proteins by 1.8±0.3 fold in human TNBC cells instead of raising them; Bioinformatics analysis and folding energy calculations revealed that mRNA targets of miR-17-5p guide strand such as PDCD4 and PTEN could also be regulated by miR-17-3p passenger strand;GFRα2 prompts cell growth and chemoresistance through down regulating tumor suppressor gene PTEN via Mir 17 5p in pancreatic cancer; Mechanically we discovered that high GFRα2 expression level leads to PTEN inactivation via enhancing Mir-17-5p level;We found that these phenotypes were the results of miR-17 targeting PTEN;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;miR 17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression; Expression of miR-17 was negatively correlated with PTEN in OS tissues;Resveratrol and pterostilbene epigenetically restore PTEN expression by targeting oncomiRs of the miR 17 family in prostate cancer; Further pterostilbene through downregulation of miR-17-5p and miR-106a-5p expression both in tumors and systemic circulation rescued PTEN mRNA and protein levels leading to reduced tumor growth in vivo;In addition ERβ expression significantly increased in calycosin-treated HCT-116 cells followed by a decrease of miR-17 and up-regulation of PTEN; Our results indicate that calycosin has an inhibitory effect on CRC which might be obtained by ERβ-mediated regulation of miR-17 and PTEN expression;The High Expression of the microRNA 17 92 Cluster and its Paralogs and the Downregulation of the Target Gene PTEN Is Associated with Primary Cutaneous B Cell Lymphoma Progression;MicroRNA 17 5p induces drug resistance and invasion of ovarian carcinoma cells by targeting PTEN signaling; miR-17-5p activates AKT by downregulation of PTEN in ovarian cancer cells;MicroRNA 17 5p promotes chemotherapeutic drug resistance and tumour metastasis of colorectal cancer by repressing PTEN expression; We found that PTEN was a target of miR-17-5p in the colon cancer cells and their context-specific interactions were responsible for multiple drug-resistance; Chemotherapy was found to increase the expression levels of miR-17-5p which further repressed PTEN levels contributing to the development of chemo-resistance; MiR-17-5p is a predictive factor for chemotherapy response and a prognostic factor for overall survival in CRC which is due to its regulation of PTEN expression;The mature miR-17-5p exerted this function by repressing the expression of PTEN ;drug resistance;;;metastasis;;;progression;drug resistance;metastasis;drug resistance;poor survival; breast cancer;pancreatic cancer;glioblastoma;sarcoma;sarcoma;prostate cancer;colorectal cancer;B cell lymphoma;ovarian cancer;colorectal cancer;liver cancer hsa-miR-181a-5p PTEN 0.635863607630183 6.72486918157744e-11 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.217453583427793 2.70335682235321e-16 24532253;27534652;24685694;27264420;26323677;26750720 LPS induced miR 181a promotes pancreatic cancer cell migration via targeting PTEN and MAP2K4; Furthermore we predicted and confirmed that both PTEN and MAP2K4 were miR-181a targets; Pancreatic cancer tissues analysis showed that PTEN and MAP2K4 were all negatively correlated with miR-181a;From our results down-regulation of miR-181a increased the expression of PTEN and decreased phosphorylation of Akt and FOXO1;miR-181a performs this function by inhibiting the expression of PTEN leading to an increase of phosphorylated AKT which triggers metabolic shift;The expression of PTEN was regulated by IL-1β-stimulated miR-181a expression; In a PTEN reporter plasmid miR-181a binding site mutations were introduced; Furthermore a siRNA strategy was used to find that IL-1B regulates miR-181a expression via NF-κB and then regulates PTEN expression; Consequently repression of PTEN by miR-181a promotes colon cancer cell proliferation;Luciferase assays were performed to assess the ability of miR-181a to target the PTEN promoter and regulation of PTEN expression by miR-181a was assessed by western blot analysis and RT-PCR; PTEN was identified as a direct target of miR-181a; Our findings demonstrate that miR-181a expression in lung adenocarcinoma is associated with EMT progression potentially through targeting of PTEN;The expression of miR-181A and PTEN in CRC patients with late liver metastases was higher than that of the normal control group whereas the expression of miR-181A and PTEN was lower in all pathological groups TNM I-TNM IV; The expression of miR-181A and PTEN in the colon cell line NCM460 was lower than that of the colon cancer SW620 cell line; Upregulation of miR-181A promoted cell viability and inhibited apoptosis of SW620 cells suppressed PTEN expression and activated phosphorylation of AKT p-AKT in SW620 cells; Additionally downregulation of miR-181A inhibited cell viability of SW620 cells through promotion of PTEN and inhibition of p-AKT cell migration;;;;progression;metastasis pancreatic cancer;cervical and endocervical cancer;colon cancer;colorectal cancer;lung cancer;colorectal cancer hsa-miR-181b-5p PTEN 1.22749257795094 1.21998198830919e-27 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;MirTarget;miRNATAP -0.223803397924892 6.72795816887185e-24 NA NA NA hsa-miR-181c-5p PTEN 0.574847839298605 4.51287502066478e-07 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.128349050965034 2.50139620016373e-08 25695913 miR 181c promotes proliferation via suppressing PTEN expression in inflammatory breast cancer; In this study we showed that miR-181c as an oncogene promoted proliferation and it inhibited PTEN protein expression by targeting 3'-UTR of PTEN mRNA in IBC SUM149 cells; Thus targeting miR-181c and restoration of PTEN can be used in conjunction with other therapies to prevent progression of IBC progression breast cancer hsa-miR-181d-5p PTEN 0.782618466877349 1.2821116358212e-09 -0.737945156702629 1.26474514880987e-22 MirTarget -0.120384368723786 2.66954476137279e-09 27006767 Moreover hsa-miR-181d was upregulated to control expression a tumor suppressor gene PTEN to protect the cancer cell from apoptosis endometrial cancer hsa-miR-18a-5p PTEN 0.969066793715868 1.69069796425892e-08 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase -0.135863155421297 1.28041670903141e-19 24681249;27291152 However higher levels of the miR-17~92 cluster switched from PTEN to oncogenes including Ctnnb1 β-catenin via miR-18a which resulted in inhibition of tumor growth and metastasis;miR 18a promotes cell proliferation of esophageal squamous cell carcinoma cells by increasing cylin D1 via regulating PTEN PI3K AKT mTOR signaling axis metastasis; colon cancer;esophageal cancer hsa-miR-19a-3p PTEN 0.817507952171848 3.76285090469625e-08 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.132652171590163 2.97639287894663e-14 27445062;24831732;21853360;26098000;24681249;27289489;25107371 The target genes of miR-19a such as ABCA1 and PTEN that had been suppressed by miR recovered their expression through CAP treatment;Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN;Transfection of miR-19a and -19b mimics reversed the up-regulations of IGF2R and PTEN gene expression and abrogated the GSE induced anti-proliferative response;The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid; Meanwhile gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN; The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN ;;drug resistance;;metastasis;drug resistance; breast cancer;breast cancer;breast cancer;lung cancer;colon cancer;lung cancer;bladder cancer hsa-miR-19b-3p PTEN 0.139124391010031 0.255658553872934 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.108487626225012 5.17000304535224e-07 24831732;21853360;26098000;24681249 Meanwhile BPA-induced upregulation of oncogenic miR-19a and miR-19b and the dysregulated expression of miR-19-related downstream proteins including PTEN p-AKT p-MDM2 p53 and proliferating cell nuclear antigen were reversed by curcumin;Regulation of miR 19 to breast cancer chemoresistance through targeting PTEN; Expression levels of miR-19 in MDR cells were inversely consistent with those of PTEN; Our findings demonstrate for the first time involvement of miR-19 in multidrug resistance through modulation of PTEN and suggest that miR-19 may be a potential target for preventing and reversing MDR in tumor cells;Moreover siRNA-mediated knockdown of PTEN a target of miR-19 also resulted in EMT migration and invasion of A549 and HCC827 cells suggesting that PTEN is involved in miR-19-induced EMT migration and invasion of lung cancer cells;miR-19 in the context of the miR-17~92 cluster at medium levels promoted tumor metastasis through induction of Wnt/β-catenin-mediated epithelial-mesenchymal transition by targeting to the tumor-suppressor gene PTEN ;drug resistance;;metastasis breast cancer;breast cancer;lung cancer;colon cancer hsa-miR-200a-3p PTEN 2.55218480823434 2.96135446816238e-51 -0.737945156702629 1.26474514880987e-22 miRNATAP -0.109411985169924 5.36101217094024e-14 22637745;24742567;21408027 Re expression of miR 200 by novel approaches regulates the expression of PTEN and MT1 MMP in pancreatic cancer; We initially compared the expression profile of miR-200 family PTEN and MT1-MMP expression in six pancreatic cancer PC cell lines by qRT-PCR and western blot analysis; We found loss of expression of miR-200a b and c in chemo-resistant PC cell lines which was correlated with loss of PTEN and over-expression of MT1-MMP; The expression of miR-200 family and PTEN was significantly re-expressed whereas the expression of MT1-MMP was down-regulated by CDF and BR-DIM treatment; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment;PTEN might be a potential target of miR-141 and miR-200a in endometrial carcinogenesis;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants ;tumorigenesis; pancreatic cancer;endometrial cancer;pancreatic cancer hsa-miR-200b-3p PTEN 2.11744072703209 1.66710572104191e-43 -0.737945156702629 1.26474514880987e-22 TargetScan;mirMAP -0.130827329200595 5.08867014435072e-16 22637745;21408027 Re expression of miR 200 by novel approaches regulates the expression of PTEN and MT1 MMP in pancreatic cancer; We initially compared the expression profile of miR-200 family PTEN and MT1-MMP expression in six pancreatic cancer PC cell lines by qRT-PCR and western blot analysis; The expression of miR-200 family and PTEN was significantly re-expressed whereas the expression of MT1-MMP was down-regulated by CDF and BR-DIM treatment; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants ; pancreatic cancer;pancreatic cancer hsa-miR-200c-3p PTEN 2.07038651806183 6.84613386286599e-51 -0.737945156702629 1.26474514880987e-22 mirMAP;miRNATAP -0.143190372668334 7.03511808200308e-16 24682933;22637745 In conclusion miR-200c functions as an oncogene in colon cancer cells through regulating tumor cell apoptosis survival invasion and metastasis as well as xenograft tumor growth through inhibition of PTEN expression and p53 phosphorylation;Forced over-expression or silencing of miR-200c followed by either CDF or BR-DIM treatment of MIAPaCa-2 cells altered the morphology of cells wound-healing capacity colony formation and the expression of MT1-MMP and PTEN; These results provide strong experimental evidence showing that the loss of miR-200 family and PTEN expression and increased level of MT1-MMP leads to aggressive behavior of PC cells which could be attenuated through re-expression of miR-200c by CDF and/or BR-DIM treatment suggesting that these agents could be useful for PC treatment metastasis;poor survival; colon cancer;pancreatic cancer hsa-miR-20a-5p PTEN 0.44979911120222 0.000602386964327228 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.21141055042385 3.32787653231297e-27 26031366 The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction; MiR-20a levels were increased in HCC cell lines and tissues whereas PTEN was inversely correlated with it; PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance drug resistance liver cancer hsa-miR-21-5p PTEN 2.32007501846389 3.08394781855554e-137 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;mirMAP -0.241962926381789 5.21884080644212e-25 26387181;21471222;26905520;25027758;21820606;24930006;22547075;26666820;23951172;25647415;22678116;22832383;19212625;22267008;26384051;22322462;27644439;21468550;20113523;21806946;24154840;27611950;24780321;23036707;23684551;26559642;26731559;26847601;27350731;24331411;25909227;24293118;26289851;24324076;23201752;17681183;25963606;27188433;22958183;22956424;19730150;21842656;21104017;23894315;23548551;26236156;24460329;20092645;25973032;25563770;24659669;26230405;25543482;20048743;27725205;23466500;22922228;25058005;20223231;27220494;24763002;24221338;22120473;21408027;23174819;22978663;25799148;26741162;23226804;26787105;26864640;26311740;25603978;26975392 In addition treated with 5-AZA resulted in significant increases of miR-21 expression in both MCF-7 and MDA-MB-231 cells P < 0.01 with the protein level of PTEN increased in MCF-7 cell which was further involved in the downregulation of AKT;Here we demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474 SKBR3 and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody whereas protein expression of the PTEN gene a miR-21 target was reduced; Rescuing PTEN expression with a p3XFLAG-PTEN-mut construct with deleted miR-21 targeting sequence at its 3' UTR restored the growth inhibition of trastuzumab in the resistant cells by inducing PTEN activation and AKT inhibition; In vivo administering miR-21 antisense oligonucleotides restored trastuzumab sensitivity in the resistant breast cancer xenografts by inducing PTEN expression whereas injection of miR-21 mimics conferred trastuzumab resistant in the sensitive breast tumors via PTEN silence;The effect of ZA on miR-21 expression was quantified by qRT-PCR and the amount of PTEN protein and its targets were analyzed by Western blot;In the same patients we also compared miR-21 expression with the expression of its presumed target PTEN; miR-21 expression levels were found to significantly correlate with tumour size r = 0.403 p = 0.009; Spearman's rank whereas no relation was found between miR-21 and PTEN expression levels Kruskal-Wallis test;MicroRNA 21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN; TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-21 and tumor suppressor gene PTEN protein; We showed that upregulation of miR-21 in MCF-7/ADR cells was concurrent with downregulation of PTEN protein; Overexpression of PTEN could mimic the same effects of miR-21 inhibitor in MCF-7/ADR cells and PTEN-siRNA could increase the resistance of MCF-7 cells to ADR; MiR-21 inhibitor could increase PTEN protein expression and the luciferase activity of a PTEN 3' untranslated region-based reporter construct in MCF-7/ADR cells; Dysregulation of miR-21 plays critical roles in the ADR resistance of breast cancer at least in part via targeting PTEN;In present study we determined the miR-21 levels in TNBC specimens and TNBC cell levels in vitro and then identified the role of miR-21 on tumor cell proliferation apoptosis and then identified PTEN as the possible target of the microRNA;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;PTEN a direct target gene of miR-21 was significantly downregulated in gemcitabine-resistant breast cancer cells and restoration of PTEN expression blocked miR-21-induced EMT and gemcitabine resistance;The results showed that knockdown of miR-21 by antagomir-21 decreased cell proliferation and induced apoptosis via targeting PTEN both in 4T1 cells and HUVECs;MiR-21 upregulation contributes to PTEN downregulation which is beneficial for the activation of PI3K/AKT signaling;Furthermore miR-21-induced upregulation of CSF-1 mRNA and its transcription were prevented by expression of PTEN mRNA lacking 3'-untranslated region UTR and miR-21 recognition sequence; Our results reveal a novel mechanism for the therapeutic function of fish oil diet that blocks miR-21 thereby increasing PTEN levels to prevent expression of CSF-1 in breast cancer;After miR-21 was transfected in MCF-7 cells PTEN protein level was measured by Western blot; Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt resulting in accumulation of Bad p21/WAF1/CIP1 and p27/KIP1;To further determine the potential involvement of miR-21 in breast cancer we have evaluated the expression level of miR-21 by stem-loop real-time RT-PCR based on SYBR-Green I in human invasive ductal carcinoma of the breast and we have correlated the results with clinicopathologic features and PTEN protein expression; The expression levels of miR-21 were correlated with PTEN and commonly used clinicopathologic features of breast cancer; Expression of miR-21 was negatively correlated with expression of PTEN P=0.013; These findings suggest that PTEN is possibly one of the targets of miR-21 in breast cancer and high expression of mir-21 indicates a more aggressive phenotype;microRNA 21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN; Thus in this study we focused on the expression and significance of miR-21 in gastric cancer tissues and the role of miR-21 in the biological behaviour and the expression of PTEN in gastric cancer cells; Western blotting and the Luciferase Reporter Assay were used to evaluate the change of PTEN expression after lowered expression of miR-21 in gastric cancer cell lines; The western blot results and Luciferase Reporter Assay demonstrated that PTEN expression was remarkably increased after miR-21 inhibition P<0.05 microRNA-21 expression was upregulated in gastric carcinoma tissues and was significantly associated with the degree of differentiation of tumour tissues local invasion and lymph node metastasis;Furthermore we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog PTEN the result of which can be rescued by miR-21 inhibitor;Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon;Bmi-1 also regulates p53 and PTEN via miR-21;The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting respectively in tumor tissues as well as adjacent non-tumor mucosa; miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues; A negative correlation between expression of miR-21 and PTEN was established in vivo;MicroRNA 21 inhibitor sensitizes human glioblastoma cells U251 PTEN mutant and LN229 PTEN wild type to taxol; Human glioblastoma U251 PTEN-mutant and LN229 PTEN wild-type cells were treated with taxol and the miR-21 inhibitor in a poly amidoamine PAMAM dendrimer alone or in combination; Interestingly the above data suggested that in both the PTEN mutant and the wild-type GBM cells miR-21 blockage increased the chemosensitivity to taxol; Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;NTR1 activation stimulates expression of miR-21 and miR-155 in colonocytes via Akt and NF-κB to down-regulate PTEN and SOCS1 and promote growth of tumors in mice;Correlations between the expression levels of miR-21 PTEN and p-AKT were analyzed by real-time PCR and Western blot test in HER2-positive GC cell lines; Overexpression of miR-21 down-regulated PTEN expression increased AKT phosphorylation and did not affect HER2 expression; Inversely suppression of miR-21 increased PTEN expression and down-regulated AKT phosphorylation but still did not affect HER2 expression;MicroRNA 21 promotes TGF β1 induced epithelial mesenchymal transition in gastric cancer through up regulating PTEN expression; In GC tissues the expressions of miR-21 Akt and p-Akt were up-regulated while PTEN expression was down-regulated; These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression;Expressions of PTEN were significantly down-regulated in CRC tissues and negatively correlated with expressions of Notch-1 r2=0.5207 p<0.01 and miR-21 r2=0.6996 p<0.01; These data indicate that the crosstalk between Notch-1 and miR-21 is involved in CRC development through degradation of PTEN;Over-expression of miR-21 suppressed its target PTEN and disrupted acinar morphogenesis;MicroRNA 21 suppresses PTEN and hSulf 1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways;There was overexpression of the miR-21 target genes PTEN by 67% and caspase-3 by 15% upon cotreatment;We previously reported that microRNA-21 miR-21 was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target PTEN; Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN stem-loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status; miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression; These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus;Triptolide reduces proliferation and enhances apoptosis of human non small cell lung cancer cells through PTEN by targeting miR 21; To the best of our knowledge the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21;Quantitative real-time PCR qRT-PCR and Western blot were used to detect the expression levels of miR-21 and PTEN in HCT116 HT29 Colo32 and SW480 CRC cell lines; Also the expression levels of PTEN mRNA and its downstream proteins AKT and PI3K in HCT116 cells after downregulating miR-21 were investigated; In comparing the levels of PTEN protein and downstream AKT and PI3K in HCT116 cells after downregulation of miR-21 expression the levels of AKT and PI3K protein expression significantly decreased P < 0.05; PTEN is one of the direct target genes of miR-21;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;MiR-21 level was inversely correlated with the levels of FOXO1 and PTEN in DLBCL cell lines; MiR-21 also down-regulated PTEN expression and consequently activated the PI3K/AKT/mTOR pathway which further decreased FOXO1 expression;MiR 21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non small cell lung cancer A549 cells; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA was performed to modulate the expression of microRNA-21 and PTEN under the treatment of curcumin; Moreover the protein level of PTEN a putative target of microRNA-21 was significantly elevated in curcumin-treated A549 cells as determined by Western blot analysis; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA significantly P < 0.05 reversed the growth suppression and apoptosis induction by curcumin compared to corresponding controls; Our data suggest a novel molecular mechanism in which inhibition of microRNA-21 and upregulation of PTEN mediate the anticancer activities of curcumin in NSCLC cells;PTEN gene expression was performed as a known target of miR-21;We also found that Rawq01 up-regulated the expression of PTEN through mir-21 inhibition and therefore inhibited the PI3K-AKT pathway;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;MicroRNA 21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer; PTEN was shown to be a direct target of miR-21 and to contribute to miR-21 effects on cell invasion; Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9 both downstream mediators of PTEN involved in cell migration and invasion; Aberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth migration and invasion;Overexpression of miR 21 promotes the proliferation and migration of cervical cancer cells via the inhibition of PTEN; The aim of this study was to examine the expression of miR-21 and PTEN in cervical cancer specimens using quantitative PCR; miR-21 was upregulated in the cervical cancer specimens negatively correlating with the PTEN mRNA level; Transfection of the miR-21 mimics was markedly promoted whereas the miR-21 inhibitor suppressed the proliferation migration and invasion of cervical cancer cells with a significant inhibition of PTEN expression; The present study showed the upregulation of miR-21 in invasive cervical cancers and confirmed the promotion of miR-21 with regard to the proliferation migration and invasion in cervical cancer cells via inhibiting the PTEN expression;The expressions of miR-21 PTEN PI3K and AKT were detected in 89 esophageal cancer samples and 58 adjacent normal tissues respectively; MiR-21 PI3K and AKT have higher expressions but PTEN has lower expression in esophageal cancer tissues compared with adjacent normal tissues; Further PTEN was a target gene of miR-21;The expression level of miR-21 and PTEN messenger RNA were measured by quantitative real-time reverse transcription polymerase chain reaction or reverse transcription polymerase chain reaction; miR-21 was overexpressed and PTEN was suppressed in established radioresistant TE-R60 cells compared with the parent cells 1.3-fold and 70.83%; The inhibition of miR-21 significantly increased the cells' radiosensitivity P < 0.05 and the PTEN protein expression 2.3-fold in TE-1 cells; Knockdown of PTEN in anti-miR-21 TE-1 cells could abrogate the miR-21 inhibition-induced radiosensitization P < 0.05; Inhibition of miR-21 increased radiosensitivity of esophageal cancer TE-1 cells and this effect was possibly through the activation of PTEN;MicroRNA 21 miR 21 expression promotes growth metastasis and chemo or radioresistance in non small cell lung cancer cells by targeting PTEN; Taken together these results provide evidence to show the promotion role of miR-21 in NSCLC development through modulation of the PTEN signaling pathway;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Role of microRNA 21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma; To evaluate the role of miR-21 and PTEN cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells; In Eca109 when transfected with miR-21 mimics accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40% which resulted in the promotion of cell proliferation; However when transfected with miR-21 inhibitor expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79% which resulted in the suppression of cell proliferation; Furthermore there was a significantly inverse correlation between miR-21 expression and PTEN protein levels p < 0.05; The author concluded that MiR-21 was overexpressed in vitro and ESCC and promoted the cell proliferation might target PTEN at post-transcriptional level and regulated the cancer invasion in Kazakh's ESCC;Difluorinated curcumin CDF restores PTEN expression in colon cancer cells by down regulating miR 21; Indeed our current data demonstrate a marked downregulation of PTEN in SCID mice xenografts of miR-21 over-expressing colon cancer HCT116 cells; Colonospheres that are highly enriched in cancer stem/stem like cells reveal increased miR-21 expression and decreased PTEN; Difluorinated curcumin CDF a novel analog of the dietary ingredient curcumin which has been shown to inhibit the growth of 5-Flurouracil + Oxaliplatin resistant colon cancer cells downregulated miR-21 in chemo-resistant colon cancer HCT116 and HT-29 cells and restored PTEN levels with subsequent reduction in Akt phosphorylation;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells;Finally invasion and metastasis assays were performed and alteration in mir-21 PTEN AKT and pAKT level was evaluated in these cells; Enhanced invasion and metastasis increased miR-21 expression decreased PTEN elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells;In the present study we investigated the role of miR-21 and its potential as a therapeutic target in two prostate cancer cell lines characterized by different miR-21 expression levels and PTEN gene status;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;Expressions of microRNA-21 miR-21 PTEN MMP9 and p47 were detected by qPCR;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;microRNA 21 Regulates Cell Proliferation and Migration and Cross Talk with PTEN and p53 in Bladder Cancer; MicroRNA-21 regulates proliferation and migration of bladder cancer cells and cross talk with PTEN and p53 in bladder cancer;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status; To explore whether miR-21 can serve as a therapeutic target for glioblastoma we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 PTEN mutant and LN229 PTEN wild-type GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo; Taken together our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated; In conclusion our data suggest that mUA can suppress cell viability in DU145 cells through modulating miR-21 and its downstream series-wound targets including PTEN Akt and Wnt/β-catenin signaling;miR 21 confers cisplatin resistance in gastric cancer cells by regulating PTEN; In addition miR-21 induced cell survival and cisplatin resistance through downregulating the expression of phosphatase and tension homolog deleted on chromosome 10 PTEN and activation of Akt pathway;Finally we demonstrate that modulation of tumor suppressors PTEN and p53 in U87 cells does not affect the decrease in miR-21 levels associated with PDGF-B overexpression;This study aimed to investigate whether NSCLC miR-21 mediated resistance to TKIs also results from Pten targeting; Here we show miR-21 promotes cancer by negatively regulating Pten expression in human NSCLC tissues: high miR-21 expression levels were associated with shorter DFS in 47 NSCLC patients; high miR-21/low Pten expression levels indicated a poor TKI clinical response and shorter overall survival in another 46 NSCLC patients undergoing TKI treatment; In vitro assays showed that miR-21 was up-regulated concomitantly to down-regulation of Pten in pc-9/GR cells in comparison with pc-9 cells; Moreover over-expression of miR-21 significantly decreased gefitinib sensitivity by down-regulating Pten expression and activating Akt and ERK pathways in pc-9 cells while miR-21 knockdown dramatically restored gefitinib sensitivity of pc-9/GR cells by up-regulation of Pten expression and inactivation of AKT and ERK pathways in vivo and in vitro;MicroRNA 21 miR 21 represses tumor suppressor PTEN and promotes growth and invasion in non small cell lung cancer NSCLC; We identified the role of miR-21 in non-small cell lung cancer NSCLC and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation; Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot respectively; Tumor tissues showed an inverse correlation between miR-21 and PTEN protein; miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines; miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC;MiR-21 overexpression decreases PTEN increases p-Akt and subsequently increases HIF-1α expression while miR-21 inhibition results in increased PTEN decreased p-Akt and then decreased HIF-1α;This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma DLBCL paraffin-embedded tissues and evaluate its potential relevance with clinical characteristics; In patients with DLBCL the expression level of miR-21 was negatively correlated with the level of PTEN protein; These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL;Down regulation of PTEN expression modulated by dysregulated miR 21 contributes to the progression of esophageal cancer; We demonstrated that knockdown of miR-21 significantly increased expression of PTEN protein; Our findings suggest that miR-21 could be a potential oncomiR probably by regulation of PTEN and a novel prognostic factor for ESCC patients;The levels of mir-21 did not associate with the expression of PTEN an important tumour suppressor in CRC and one of many putative targets of miR-21 but interestingly was associated with stage of disease in the PTEN expressing tumours;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants;The PTEN protein levels in CRC tissues and cells had an inverse correlation with miR-21 expression; MiR-21 targets PTEN at the post-transcriptional level and regulates cell proliferation and invasion in CRC;These findings demonstrate a novel role of AR in the regulation of miR-21 and its target PTEN in growth factor-induced colon cancer cell growth;Whether miR-21 regulated PTEN expression was assessed by luciferase assay; The RNA and protein levels of PTEN were significantly decreased by exogenous miR-21 and the 3'-untranslated region of PTEN was shown to be a target of miR-21;In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX;microRNA 21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer; We performed a qRT-PCR assay with miR-21 and PTEN in 16 paired EEC tumor tissues and adjacent non-tumor endometrium; To validate the putative binding site of miR-21 in the 3' untranslated region 3'-UTR of PTEN messenger RNA mRNA a dual-luciferase reporter assay was carried out; The upregulation of miR-21 led to a significant decrease in the PTEN protein expression level P=0.007; The downregulation of miR-21 led to a significant increase in PTEN protein P=0.002; In conclusion we demonstrated that the expression of PTEN protein but not mRNA was negatively directly regulated by miR-21 in the KLE cell line; The overexpression of miR-21 modulated EEC cell proliferation through the downregulation of PTEN;The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR 21 and promoter methylation; In this study we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC; PTEN mRNA level was negatively correlated with miR-21 level r = -0.595 P < 0.001; PTEN expression was also correlated directly with the PTEN mRNA level r = 0.583 P < 0.001 and conversely with miR-21 level r = -0.632 P < 0.001; This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;Exposure of HCC cells to sorafenib led to an increase in miR-21 expression a decrease in PTEN expression and sequential Akt activation;MicroRNA 21 controls hTERT via PTEN in human colorectal cancer cell proliferation; The aim of this study was to determine a role of microRNA-21 miRNA-21 in colorectal cancer CRC and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue PTEN;Relevance of miR 21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells; We identified the tumor suppressor gene PTEN as a target of miR-21 and determined the mechanism of its regulation throughout reporter construct plasmids; Using this model we analyzed the expression of miR-21 and PTEN as well as functional effects such as autophagy and apoptosis induction; In SiHa cells there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells; We conclude that miR-21 post-transcriptionally down-regulates the expression of PTEN to promote cell proliferation and cervical cancer cell survival ;drug resistance;;;drug resistance;;;drug resistance;;;;;;metastasis;differentiation;;tumorigenesis;;;;;;;;;progression;;;;;drug resistance;;;;;;cell migration;;;;metastasis;drug resistance;;;;;;;metastasis;;;;;;;progression;malignant trasformation;;drug resistance;poor survival;;drug resistance;poor survival;;;;progression;staging;;;;;;;;drug resistance;;;poor survival breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;breast cancer;gastric cancer;cervical and endocervical cancer;colon cancer;gastric cancer;bladder cancer;glioblastoma;colon cancer;gastric cancer;gastric cancer;colorectal cancer;lung cancer;liver cancer;glioblastoma;melanoma;lung squamous cell cancer;colorectal cancer;lung squamous cell cancer;B cell lymphoma;lung squamous cell cancer;thyroid cancer;esophageal cancer;glioblastoma;liver cancer;cervical and endocervical cancer;esophageal cancer;esophageal cancer;lung squamous cell cancer;pancreatic cancer;lung cancer;esophageal cancer;colon cancer;B cell lymphoma;colon cancer;pancreatic cancer;prostate cancer;liver cancer;lung cancer;gastric cancer;bladder cancer;B cell lymphoma;glioblastoma;prostate cancer;gastric cancer;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;cervical and endocervical cancer;B cell lymphoma;esophageal cancer;colorectal cancer;pancreatic cancer;colorectal cancer;colon cancer;lung squamous cell cancer;lung cancer;endometrial cancer;colorectal cancer;pancreatic cancer;liver cancer;colorectal cancer;cervical and endocervical cancer hsa-miR-25-3p PTEN -0.00546790775985073 0.944061771389468 -0.737945156702629 1.26474514880987e-22 miRTarBase;MirTarget;miRNATAP -0.2154254166509 2.47655742416089e-10 NA NA NA hsa-miR-30d-3p PTEN 0.264544633563642 0.00755023189129747 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.168386426148969 2.57365382931766e-10 NA NA NA hsa-miR-30d-5p PTEN -0.247082219011045 0.00406717930063148 -0.737945156702629 1.26474514880987e-22 mirMAP -0.119749089269445 0.000108226912832517 NA NA NA hsa-miR-30e-5p PTEN 0.293491764699475 0.000372291967271834 -0.737945156702629 1.26474514880987e-22 mirMAP -0.102649949652368 0.00147806085442833 NA NA NA hsa-miR-32-5p PTEN 1.38676603945192 2.29428299685976e-34 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.17719636259429 1.05244002548292e-15 24123284;25647261;23617834 In this study we determined the levels of the correlation between and the clinical significance of the expression of miR-32 and phosphatase and tensin homologue PTEN a tumor suppressor targeted by miR-32 in CRC; The levels of miR-32 and PTEN gene expression in 35 colorectal carcinoma samples 35 corresponding cancer-adjacent tissue samples 27 colorectal adenoma samples and 16 normal tissue samples were quantified using real-time quantitative reverse transcriptase-polymerase chain reaction; The relationship between the miR-32 and PTEN protein expression and clinicopathological factors was analyzed; Significant upregulation of miR-32 expression and reduction of PTEN were identified in CRC tissues; An inverse relationship between miR-32 and PTEN protein expression was identified; MiR-32 and PTEN expression were inversely correlated and miR-32 may be associated with the development of CRC;MiR 32 induces cell proliferation migration and invasion in hepatocellular carcinoma by targeting PTEN; Besides miRNA-32 down-regulates PTEN through binding to 3'-UTR of PTEN mRNA from luciferase reporter assay and the expression level of miR-32 could affect the proliferation migration and invasion of liver cancer cell lines via PTEN/Akt signaling pathway; Down-expression of PTEN could significantly attenuate the inhibitory effects of knockdown miR-32 on the proliferation migration and invasion of liver cancer cells suggesting that miR-32 could be a potential target for HCC treatment;MicroRNA 32 miR 32 regulates phosphatase and tensin homologue PTEN expression and promotes growth migration and invasion in colorectal carcinoma cells; In this study we identified the potential effects of miR-32 on some important biological properties of CRC cells and clarified the regulation of PTEN by miR-32; The 3'-untranslated region 3'-UTR of PTEN combined with miR-32 was verified by dual-luciferase reporter assay; Gain-of-function and loss-of-function studies showed that overexpression of miR-32 promoted SW480 cell proliferation migration and invasion reduced apoptosis and resulted in downregulation of PTEN at a posttranscriptional level; However miR-32 knock-down inhibited these processes in HCT-116 cells and enhanced the expression of PTEN protein; In addition we further identified PTEN as the functional downstream target of miR-32 by directly targeting the 3'-UTR of PTEN; Our results demonstrated that miR-32 was involved in tumorigenesis of CRC at least in part by suppression of PTEN ;;tumorigenesis colorectal cancer;liver cancer;colorectal cancer hsa-miR-340-5p PTEN 1.0857159627596 4.94471062546756e-24 -0.737945156702629 1.26474514880987e-22 miRNATAP -0.219336420279721 1.16026230714932e-20 NA NA NA hsa-miR-374a-5p PTEN -0.239709888246804 0.00309819667153627 -0.737945156702629 1.26474514880987e-22 MirTarget;mirMAP -0.10880061073271 0.000942835734329093 NA NA NA hsa-miR-429 PTEN 2.84108892259147 3.88520896426515e-64 -0.737945156702629 1.26474514880987e-22 PITA;miRanda;mirMAP;miRNATAP -0.13365636792818 1.00274002812788e-20 NA NA NA hsa-miR-455-5p PTEN 0.284641224662773 0.0357572339594277 -0.737945156702629 1.26474514880987e-22 miRanda -0.18068645365542 3.51124729527242e-21 NA NA NA hsa-miR-532-5p PTEN 0.0386976222860582 0.682018574136515 -0.737945156702629 1.26474514880987e-22 PITA;mirMAP;miRNATAP -0.207204663361429 1.01792292857881e-13 NA NA NA hsa-miR-542-3p PTEN 0.568822103820889 8.5699075257651e-07 -0.737945156702629 1.26474514880987e-22 PITA;miRanda;miRNATAP -0.120703250512885 1.08830015884254e-07 NA NA NA hsa-miR-590-3p PTEN 1.27438512890514 1.03164723481469e-23 -0.737945156702629 1.26474514880987e-22 MirTarget;PITA;miRanda;mirMAP -0.130863097442091 5.26569206971815e-10 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting; miR-590-3p was found to activate PI3K-AKT signaling pathway by down-regulating PTEN to promote AKT1-S473 phosphorylation liver cancer hsa-miR-590-5p PTEN 0.746515607868274 8.51300479640125e-09 -0.737945156702629 1.26474514880987e-22 mirMAP -0.110007008517724 5.05737696379977e-08 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-92a-3p PTEN -0.342420481092807 0.000732275969501111 -0.737945156702629 1.26474514880987e-22 MirTarget;miRNATAP -0.102588930556491 8.69033110484314e-05 26432332;25515201;24137349;23546593;23133552;24026406 Downregulation of PTEN could mimic the same effects of miR-92a mimic in NSCLC cells and rescue the effects on NSCLC cells induced by miR-92a inhibitor; Taken together these findings suggested that miR-92a could promote growth metastasis and chemoresistance in NSCLC cells at least partially by targeting PTEN;MiR 92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN Mediated PI3K/AKT Pathway; The expression of miR-92a PTEN and E-cadherin was analyzed by real-time PCR; In addition there was a negative correlation between levels of miR-92a and the PTEN gene p < 0.0001; The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models;MicroRNA miR-92 is overexpressed in a number of tumors and has been proven to negatively regulate a number of tumor suppressor genes including phosphatase and tensin homologue PTEN; PTEN protein expression was decreased in the SiHa cells that were transfected with the miR-92 mimic; The data indicated that miR-92 may increase the migration and invasion of SiHa cells partially through the downregulation of PTEN protein expression;Expression and significance of PTEN and miR 92 in hepatocellular carcinoma; Immunohistochemistry streptavidin-peroxidase SP and quantitative reverse transcriptase-polymerase chain reaction qRT‑PCR were used to detect the expression of PTEN and miR-92 in 15 cases of HCC and the corresponding paracancerous tissues; The correlation between PTEN and miR-92 was analyzed; Additionally the mRNA levels of PTEN and miR-92 showed a significantly negative correlation with each other r=-0.858 P<0.05; In conclusion PTEN and miR-92 have different roles in the development of HCC; The combined detection of PTEN and miR-92 may provide critical clinical evidence for the early diagnosis and prognosis of HCC;PTEN mRNA correlated inversely with miR-92a and members of the miR-17 and miR-130/301 families;The expression levels of miR-92a and phosphatase and tensin homologue PTEN were detected by qRT-PCR and western blot; In addition the regulation of PTEN by miR-92a was evaluated by qRT-PCR western blot and luciferase reporter assays; There was an inverse correlation between the levels of miR-92a and PTEN in CRC tissues; The overexpression of miR-92a in CRC cells decreased PTEN expression at the translational level and decreased PTEN-driven luciferase-reporter activity; Our results demonstrated that miR-92a induced EMT and regulated cell growth migration and invasion in the SW480 cells at least partially via suppression of PTEN expression metastasis;drug resistance;metastasis;cell migration;;worse prognosis;; lung squamous cell cancer;colorectal cancer;cervical and endocervical cancer;liver cancer;sarcoma;colorectal cancer hsa-miR-93-5p PTEN 1.01788600209314 1.24312921618126e-21 -0.737945156702629 1.26474514880987e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.264691004436426 2.0924597171148e-29 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-miR-146b-5p RCHY1 0.631041661167954 6.20366593452965e-08 -0.360026537699072 6.97467400612233e-10 PITA;miRanda -0.112832784601742 3.43076817062121e-11 NA NA NA hsa-miR-361-5p RCHY1 -0.0996867547900457 0.095428250126326 -0.360026537699072 6.97467400612233e-10 MirTarget;miRanda;miRNATAP -0.21078060434635 5.73953873822167e-10 NA NA NA hsa-miR-195-5p RFWD2 -1.47508108420708 2.20685847368258e-38 0.47600838868947 1.69778221480639e-18 MirTarget -0.14221603521867 5.99592758354006e-20 NA NA NA hsa-miR-375 RPRM 2.09893673049766 1.68351780396203e-16 -0.339849576303362 0.160381920538011 miRanda -0.126176516126823 7.46235837107678e-05 NA NA NA hsa-let-7a-5p RRM2 -0.210286642041636 0.0072546234707717 3.65726732089609 1.21993675459232e-90 miRNAWalker2_validate;TargetScan;miRNATAP -0.688360306593176 6.03676567325578e-15 NA NA NA hsa-let-7b-5p RRM2 -0.54215483448257 7.21347617597858e-08 3.65726732089609 1.21993675459232e-90 miRNAWalker2_validate;miRNATAP -0.589850326202052 2.60167780571913e-18 NA NA NA hsa-miR-100-5p RRM2 -1.89453309391092 5.00588682382388e-51 3.65726732089609 1.21993675459232e-90 miRNAWalker2_validate -0.695392390887873 1.34481957415168e-45 NA NA NA hsa-miR-125a-5p RRM2 -0.635694700253268 3.6703235721638e-11 3.65726732089609 1.21993675459232e-90 miRanda -0.34143644188529 1.89670704425452e-06 NA NA NA hsa-miR-199a-5p RRM2 -0.314703593105081 0.00909304983051124 3.65726732089609 1.21993675459232e-90 miRanda -0.397283000215991 3.4814658136539e-12 NA NA NA hsa-miR-199b-5p RRM2 -1.37022396448377 3.25758057539223e-26 3.65726732089609 1.21993675459232e-90 miRanda -0.582552015596119 5.739617883898e-31 NA NA NA hsa-miR-217 RRM2 -0.616728851115349 8.64977119332241e-05 3.65726732089609 1.21993675459232e-90 miRanda -0.153453229519576 0.000510374595648141 NA NA NA hsa-miR-26a-5p RRM2 -0.481180084102235 1.82368246174758e-10 3.65726732089609 1.21993675459232e-90 miRNAWalker2_validate -0.786165460169366 3.20020491314031e-18 NA NA NA hsa-miR-30a-5p RRM2 -0.392489994825279 0.00954894667761676 3.65726732089609 1.21993675459232e-90 miRNAWalker2_validate -0.531728004249807 2.16793404070115e-33 NA NA NA hsa-miR-30c-5p RRM2 -0.393222674580066 8.06961465717306e-05 3.65726732089609 1.21993675459232e-90 miRNAWalker2_validate -0.38448471107286 3.03327990253082e-08 NA NA NA hsa-miR-146b-5p RRM2B 0.631041661167954 6.20366593452965e-08 0.104156612356596 0.252644515445598 miRanda;miRNATAP -0.181568335844069 4.93229384883781e-12 NA NA NA hsa-miR-28-5p RRM2B -0.14267073663385 0.0497619246260571 0.104156612356596 0.252644515445598 miRanda -0.182015578311515 2.50011313753227e-05 NA NA NA hsa-miR-361-5p RRM2B -0.0996867547900457 0.095428250126326 0.104156612356596 0.252644515445598 miRanda -0.336248273333486 1.53605695065211e-10 NA NA NA hsa-miR-369-3p RRM2B 0.0880848299432269 0.48519534195924 0.104156612356596 0.252644515445598 mirMAP -0.143061008060551 6.99542748037932e-09 NA NA NA hsa-miR-103a-3p SERPINB5 0.610673390034345 1.63113920514646e-12 -1.88990089317228 6.17848613900941e-08 MirTarget -0.625762002671437 5.78489581994992e-06 NA NA NA hsa-miR-107 SERPINB5 0.678998026350662 2.56224148930538e-16 -1.88990089317228 6.17848613900941e-08 MirTarget;miRanda -0.677621932867444 2.25338980433485e-06 NA NA NA hsa-miR-16-5p SERPINB5 0.46835991343556 1.91125356547776e-06 -1.88990089317228 6.17848613900941e-08 miRNAWalker2_validate -0.513223420954364 2.65901380502442e-05 NA NA NA hsa-miR-338-3p SERPINB5 0.0799903526884966 0.575137668326679 -1.88990089317228 6.17848613900941e-08 miRanda -0.279617390725405 0.000948348328001625 NA NA NA hsa-miR-30a-5p SERPINE1 -0.392489994825279 0.00954894667761676 1.12049888044954 7.60138805308414e-13 miRNATAP -0.145922595645178 4.52059974009477e-05 NA NA NA hsa-miR-30b-5p SERPINE1 -0.46540603860146 2.4709940429591e-05 1.12049888044954 7.60138805308414e-13 MirTarget -0.308253693084862 2.51129673053519e-10 25170877 miR-30b may function as a novel tumor suppressor gene in gastric cancer by targeting PAI-1 and regulating the apoptosis of cancer cells gastric cancer hsa-miR-30c-5p SERPINE1 -0.393222674580066 8.06961465717306e-05 1.12049888044954 7.60138805308414e-13 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.300227762852185 2.96907430143248e-08 NA NA NA hsa-miR-30d-5p SERPINE1 -0.247082219011045 0.00406717930063148 1.12049888044954 7.60138805308414e-13 miRNATAP -0.37317010013897 3.19444120977288e-09 NA NA NA hsa-miR-146b-5p SESN1 0.631041661167954 6.20366593452965e-08 -0.952506930201764 9.2781623761265e-26 miRanda -0.159806500961066 3.99510319189146e-09 NA NA NA hsa-miR-15a-5p SESN1 0.812445324157528 4.50263417099822e-20 -0.952506930201764 9.2781623761265e-26 MirTarget;miRNATAP -0.170800883792492 1.43195025061269e-06 NA NA NA hsa-miR-15b-5p SESN1 0.788659356634696 1.13678211092534e-11 -0.952506930201764 9.2781623761265e-26 MirTarget;miRNATAP -0.235856134163554 1.2794534100372e-18 NA NA NA hsa-miR-16-5p SESN1 0.46835991343556 1.91125356547776e-06 -0.952506930201764 9.2781623761265e-26 MirTarget;miRNATAP -0.201519085347779 4.31408936340524e-10 NA NA NA hsa-miR-183-5p SESN1 2.95681814393872 1.09965777344285e-92 -0.952506930201764 9.2781623761265e-26 MirTarget -0.161098381239242 9.03230688259721e-17 NA NA NA hsa-miR-200b-3p SESN1 2.11744072703209 1.66710572104191e-43 -0.952506930201764 9.2781623761265e-26 MirTarget;TargetScan -0.15680863005288 9.5895183779347e-16 NA NA NA hsa-miR-200c-3p SESN1 2.07038651806183 6.84613386286599e-51 -0.952506930201764 9.2781623761265e-26 MirTarget;miRNATAP -0.201332360113161 3.52294439599536e-21 24791940 We found that miR-200c overexpression increased cellular radiosensitivity by direct regulation of the oxidative stress response genes PRDX2 GAPB/Nrf2 and SESN1 in ways that inhibits DNA double-strand breaks repair increase levels of reactive oxygen species and upregulate p21 drug resistance lung cancer hsa-miR-21-5p SESN1 2.32007501846389 3.08394781855554e-137 -0.952506930201764 9.2781623761265e-26 miRNAWalker2_validate -0.28257542976425 2.54012028934313e-23 NA NA NA hsa-miR-24-3p SESN1 -0.120123317289941 0.21432376743243 -0.952506930201764 9.2781623761265e-26 miRNAWalker2_validate;MirTarget;miRNATAP -0.224261942273344 1.02072153184188e-11 NA NA NA hsa-miR-28-5p SESN1 -0.14267073663385 0.0497619246260571 -0.952506930201764 9.2781623761265e-26 PITA;miRanda;miRNATAP -0.176225999962169 7.4172729512754e-05 NA NA NA hsa-miR-320b SESN1 0.412787504747938 0.00113457276903985 -0.952506930201764 9.2781623761265e-26 miRanda -0.139336322150339 3.40900042539742e-08 NA NA NA hsa-miR-429 SESN1 2.84108892259147 3.88520896426515e-64 -0.952506930201764 9.2781623761265e-26 MirTarget;PITA;miRanda;miRNATAP -0.171716505184115 2.54156813302447e-23 NA NA NA hsa-miR-501-3p SESN1 0.0448022813406874 0.711710441403614 -0.952506930201764 9.2781623761265e-26 MirTarget;miRNATAP -0.194789368005451 1.02513685101388e-13 NA NA NA hsa-miR-502-3p SESN1 0.11461741546458 0.276553832470138 -0.952506930201764 9.2781623761265e-26 MirTarget;miRNATAP -0.237355687602817 3.25611404239524e-15 NA NA NA hsa-miR-590-3p SESN1 1.27438512890514 1.03164723481469e-23 -0.952506930201764 9.2781623761265e-26 miRanda -0.219068274204086 3.67643342644057e-18 NA NA NA hsa-miR-590-5p SESN1 0.746515607868274 8.51300479640125e-09 -0.952506930201764 9.2781623761265e-26 miRanda -0.208439399604512 5.19778443363428e-18 NA NA NA hsa-miR-23a-3p SESN2 -0.217176729033818 0.00962615056202409 0.0242125387595751 0.743174494166211 miRNATAP -0.116396403044201 0.000119306066189182 NA NA NA hsa-miR-30e-3p SESN2 -0.708952170420973 1.05866470714978e-22 0.0242125387595751 0.743174494166211 mirMAP -0.144124810511822 2.60217787022017e-05 NA NA NA hsa-miR-106b-5p SESN3 1.22477789913175 2.31787213143326e-32 -1.56636935086919 2.7817870595808e-14 MirTarget -0.480931761551278 3.20821122590517e-13 NA NA NA hsa-miR-130b-3p SESN3 1.38122163823846 1.00932179737836e-26 -1.56636935086919 2.7817870595808e-14 mirMAP -0.173982273145392 0.0013141434394619 NA NA NA hsa-miR-17-5p SESN3 0.599531757298529 1.07318660415906e-05 -1.56636935086919 2.7817870595808e-14 MirTarget;TargetScan -0.153205413786033 0.00349837669835945 NA NA NA hsa-miR-181a-5p SESN3 0.635863607630183 6.72486918157744e-11 -1.56636935086919 2.7817870595808e-14 MirTarget -0.350744289619962 1.44407504817546e-06 NA NA NA hsa-miR-181b-5p SESN3 1.22749257795094 1.21998198830919e-27 -1.56636935086919 2.7817870595808e-14 MirTarget -0.332226990813302 6.079302129298e-08 NA NA NA hsa-miR-182-5p SESN3 2.36408084103818 2.60011457600281e-72 -1.56636935086919 2.7817870595808e-14 mirMAP -0.294879951435881 2.49105093025748e-09 NA NA NA hsa-miR-200a-5p SESN3 2.58844508672328 1.79650654234569e-56 -1.56636935086919 2.7817870595808e-14 mirMAP -0.123238417965444 0.00255673729040383 NA NA NA hsa-miR-200b-3p SESN3 2.11744072703209 1.66710572104191e-43 -1.56636935086919 2.7817870595808e-14 MirTarget;TargetScan -0.212089757301576 1.5907832953698e-06 NA NA NA hsa-miR-200c-3p SESN3 2.07038651806183 6.84613386286599e-51 -1.56636935086919 2.7817870595808e-14 MirTarget -0.280493924637921 7.03401362281759e-09 NA NA NA hsa-miR-20a-5p SESN3 0.44979911120222 0.000602386964327228 -1.56636935086919 2.7817870595808e-14 MirTarget -0.280202199348608 2.52472408800842e-07 NA NA NA hsa-miR-25-3p SESN3 -0.00546790775985073 0.944061771389468 -1.56636935086919 2.7817870595808e-14 MirTarget;miRNATAP -0.323362577673532 0.000495879180444264 NA NA NA hsa-miR-301a-3p SESN3 1.32170168375632 2.12280368957514e-15 -1.56636935086919 2.7817870595808e-14 mirMAP -0.191542703562584 6.41712671798388e-06 NA NA NA hsa-miR-30d-3p SESN3 0.264544633563642 0.00755023189129747 -1.56636935086919 2.7817870595808e-14 mirMAP -0.2629242101357 0.000293658487872284 NA NA NA hsa-miR-32-5p SESN3 1.38676603945192 2.29428299685976e-34 -1.56636935086919 2.7817870595808e-14 MirTarget;miRNATAP -0.260601204348771 1.68915772919324e-05 NA NA NA hsa-miR-340-5p SESN3 1.0857159627596 4.94471062546756e-24 -1.56636935086919 2.7817870595808e-14 mirMAP -0.460484707272809 8.1306026738168e-13 NA NA NA hsa-miR-361-5p SESN3 -0.0996867547900457 0.095428250126326 -1.56636935086919 2.7817870595808e-14 mirMAP -0.590592622345355 1.16882373119848e-06 NA NA NA hsa-miR-429 SESN3 2.84108892259147 3.88520896426515e-64 -1.56636935086919 2.7817870595808e-14 MirTarget -0.21526281577192 4.59928855164874e-08 NA NA NA hsa-miR-454-3p SESN3 1.28177672191095 6.84024979484587e-21 -1.56636935086919 2.7817870595808e-14 mirMAP -0.270981143321837 1.25324426173994e-07 NA NA NA hsa-miR-508-3p SESN3 0.260698649973977 0.189926238144551 -1.56636935086919 2.7817870595808e-14 mirMAP -0.141985664281639 9.0027412732537e-05 NA NA NA hsa-miR-589-5p SESN3 0.367182541239722 1.67804815542873e-05 -1.56636935086919 2.7817870595808e-14 MirTarget -0.475767789205014 1.30139943332495e-08 NA NA NA hsa-miR-92b-3p SESN3 1.30248132773644 9.42428674470985e-25 -1.56636935086919 2.7817870595808e-14 MirTarget;miRNATAP -0.183158227761317 0.000857475885158517 NA NA NA hsa-miR-93-5p SESN3 1.01788600209314 1.24312921618126e-21 -1.56636935086919 2.7817870595808e-14 MirTarget -0.531451947599067 2.13169451810492e-16 NA NA NA hsa-miR-96-5p SESN3 3.24596558166606 1.92296159041707e-98 -1.56636935086919 2.7817870595808e-14 MirTarget;TargetScan -0.289391385796295 1.42047141837302e-12 NA NA NA hsa-miR-92a-3p SHISA5 -0.342420481092807 0.000732275969501111 0.700544975706021 1.7828149878488e-28 miRNAWalker2_validate -0.155045227242516 1.36095419851099e-12 NA NA NA hsa-miR-15a-5p SIAH1 0.812445324157528 4.50263417099822e-20 -0.165120120542028 0.00588542147742729 miRNATAP -0.119160033954367 1.42207849374965e-07 NA NA NA hsa-let-7a-5p STEAP3 -0.210286642041636 0.0072546234707717 -0.241327584609971 0.0330320221946543 TargetScan -0.131539859878858 0.00843985511994602 NA NA NA hsa-miR-107 STEAP3 0.678998026350662 2.56224148930538e-16 -0.241327584609971 0.0330320221946543 miRanda -0.1453915745545 0.00167401736966618 NA NA NA hsa-miR-155-5p THBS1 1.09766314984151 4.55650930885814e-15 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate;mirMAP -0.133542323510001 7.64645600006924e-06 NA NA NA hsa-miR-16-1-3p THBS1 1.30142784811196 2.53343713753744e-29 -0.0614785645873859 0.62138763262321 MirTarget -0.140131478293771 0.000198601030104097 NA NA NA hsa-miR-17-5p THBS1 0.599531757298529 1.07318660415906e-05 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.211166755871651 7.35327505985432e-12 NA NA NA hsa-miR-181a-5p THBS1 0.635863607630183 6.72486918157744e-11 -0.0614785645873859 0.62138763262321 mirMAP -0.150019822568732 0.000545990600769292 NA NA NA hsa-miR-181b-5p THBS1 1.22749257795094 1.21998198830919e-27 -0.0614785645873859 0.62138763262321 mirMAP -0.1533233095909 2.76267327611462e-05 NA NA NA hsa-miR-181c-5p THBS1 0.574847839298605 4.51287502066478e-07 -0.0614785645873859 0.62138763262321 mirMAP -0.147290024790751 7.4630964763336e-05 NA NA NA hsa-miR-18a-5p THBS1 0.969066793715868 1.69069796425892e-08 -0.0614785645873859 0.62138763262321 MirTarget -0.181152396860618 8.69562380186631e-14 NA NA NA hsa-miR-19a-3p THBS1 0.817507952171848 3.76285090469625e-08 -0.0614785645873859 0.62138763262321 MirTarget;mirMAP -0.214042484472341 2.35179044480412e-14 NA NA NA hsa-miR-19b-3p THBS1 0.139124391010031 0.255658553872934 -0.0614785645873859 0.62138763262321 MirTarget;mirMAP -0.25361005913269 1.79395476818e-13 NA NA NA hsa-miR-20a-5p THBS1 0.44979911120222 0.000602386964327228 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.30952947364433 1.36418642869234e-22 NA NA NA hsa-miR-32-5p THBS1 1.38676603945192 2.29428299685976e-34 -0.0614785645873859 0.62138763262321 mirMAP -0.19932792247749 2.72222895176776e-08 NA NA NA hsa-miR-455-5p THBS1 0.284641224662773 0.0357572339594277 -0.0614785645873859 0.62138763262321 miRanda -0.181790591068211 5.96114242364848e-09 NA NA NA hsa-miR-500a-5p THBS1 0.209805801129289 0.119177819862398 -0.0614785645873859 0.62138763262321 mirMAP -0.167786316949011 1.51589228334337e-07 NA NA NA hsa-miR-590-3p THBS1 1.27438512890514 1.03164723481469e-23 -0.0614785645873859 0.62138763262321 miRanda -0.175386342682792 2.46112499417128e-07 NA NA NA hsa-miR-590-5p THBS1 0.746515607868274 8.51300479640125e-09 -0.0614785645873859 0.62138763262321 miRanda;mirMAP -0.167667995453039 2.43506908893591e-07 NA NA NA hsa-miR-671-5p THBS1 1.55248047544015 7.7377784125886e-33 -0.0614785645873859 0.62138763262321 MirTarget -0.149992236442967 2.76078556306308e-06 NA NA NA hsa-miR-92a-3p THBS1 -0.342420481092807 0.000732275969501111 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.236223996163107 1.60863345703223e-08 NA NA NA hsa-miR-98-5p THBS1 0.656336869298973 9.03113188590466e-15 -0.0614785645873859 0.62138763262321 miRNAWalker2_validate -0.196108417985267 8.06107193430326e-05 NA NA NA hsa-miR-142-3p TP73 1.53951237846104 2.06769936282034e-17 0.496595627051834 0.00711519755707105 miRanda -0.108361163843328 0.00152822881326152 NA NA NA hsa-miR-17-5p TP73 0.599531757298529 1.07318660415906e-05 0.496595627051834 0.00711519755707105 TargetScan -0.203686242683378 1.02722430405108e-05 NA NA NA hsa-miR-185-3p TP73 0.86591374114446 7.8790769678542e-10 0.496595627051834 0.00711519755707105 mirMAP -0.183884886307054 4.66034322076237e-05 NA NA NA hsa-miR-18a-5p TP73 0.969066793715868 1.69069796425892e-08 0.496595627051834 0.00711519755707105 mirMAP -0.22065384786784 1.19104385169831e-09 NA NA NA hsa-miR-193a-3p TP73 0.310617999804917 0.00477640270974957 0.496595627051834 0.00711519755707105 mirMAP -0.197004379067467 0.000621164084168105 NA NA NA hsa-miR-193a-5p TP73 -1.1508321886378 7.11949564429669e-37 0.496595627051834 0.00711519755707105 miRNAWalker2_validate;miRTarBase -0.351916782344534 1.29390251794395e-07 NA NA NA hsa-miR-193b-3p TP73 -0.0550374855074773 0.65382485898551 0.496595627051834 0.00711519755707105 mirMAP -0.157254709074547 0.00236776333910672 NA NA NA hsa-miR-193b-5p TP73 -0.481487407783336 0.000206895781789619 0.496595627051834 0.00711519755707105 mirMAP -0.198931618079958 4.35355859092893e-05 NA NA NA hsa-miR-22-3p TP73 -0.505819481364718 1.90494905330622e-13 0.496595627051834 0.00711519755707105 mirMAP -0.759467448867156 3.55463657661265e-17 NA NA NA hsa-miR-24-3p TP73 -0.120123317289941 0.21432376743243 0.496595627051834 0.00711519755707105 mirMAP -0.467949268895104 5.91964089212465e-13 NA NA NA hsa-miR-335-5p TP73 -2.61355235628987 2.6462884165886e-51 0.496595627051834 0.00711519755707105 mirMAP -0.10921364812323 0.00145304472317377 NA NA NA hsa-miR-455-3p TP73 0.678392920628872 0.000327758844555877 0.496595627051834 0.00711519755707105 miRNATAP -0.185388951410672 2.32743990387076e-08 NA NA NA hsa-miR-455-5p TP73 0.284641224662773 0.0357572339594277 0.496595627051834 0.00711519755707105 miRanda -0.137732293809756 0.00323425781963572 NA NA NA hsa-miR-590-3p TP73 1.27438512890514 1.03164723481469e-23 0.496595627051834 0.00711519755707105 miRanda -0.165559321629956 0.00110203699587496 NA NA NA hsa-miR-93-3p TP73 0.627759242044891 3.62527588304617e-06 0.496595627051834 0.00711519755707105 mirMAP -0.157246835549092 0.000717329988883304 NA NA NA hsa-miR-106b-5p ZMAT3 1.22477789913175 2.31787213143326e-32 -0.969402662641862 2.08248828196627e-18 mirMAP -0.394558217846196 1.32747215987223e-29 NA NA NA hsa-miR-130b-3p ZMAT3 1.38122163823846 1.00932179737836e-26 -0.969402662641862 2.08248828196627e-18 MirTarget -0.103491944073194 0.000403669870793951 NA NA NA hsa-miR-15a-5p ZMAT3 0.812445324157528 4.50263417099822e-20 -0.969402662641862 2.08248828196627e-18 MirTarget -0.509599203756171 4.59935686627913e-35 NA NA NA hsa-miR-15b-5p ZMAT3 0.788659356634696 1.13678211092534e-11 -0.969402662641862 2.08248828196627e-18 MirTarget -0.149159553195035 5.8561277079558e-06 NA NA NA hsa-miR-17-5p ZMAT3 0.599531757298529 1.07318660415906e-05 -0.969402662641862 2.08248828196627e-18 mirMAP -0.180725205491339 1.24056259537915e-10 NA NA NA hsa-miR-182-5p ZMAT3 2.36408084103818 2.60011457600281e-72 -0.969402662641862 2.08248828196627e-18 mirMAP -0.210572682512156 2.02994355290918e-15 NA NA NA hsa-miR-19a-3p ZMAT3 0.817507952171848 3.76285090469625e-08 -0.969402662641862 2.08248828196627e-18 MirTarget -0.186757655769112 2.72469422201923e-13 NA NA NA hsa-miR-19b-3p ZMAT3 0.139124391010031 0.255658553872934 -0.969402662641862 2.08248828196627e-18 MirTarget -0.161678846713844 3.0942718550039e-07 NA NA NA hsa-miR-200b-3p ZMAT3 2.11744072703209 1.66710572104191e-43 -0.969402662641862 2.08248828196627e-18 MirTarget;TargetScan -0.199434002125701 2.48176000863477e-17 NA NA NA hsa-miR-200c-3p ZMAT3 2.07038651806183 6.84613386286599e-51 -0.969402662641862 2.08248828196627e-18 MirTarget -0.202683466626764 6.09304654624938e-15 NA NA NA hsa-miR-20a-5p ZMAT3 0.44979911120222 0.000602386964327228 -0.969402662641862 2.08248828196627e-18 mirMAP -0.288925605829564 9.09809243968709e-24 NA NA NA hsa-miR-301a-3p ZMAT3 1.32170168375632 2.12280368957514e-15 -0.969402662641862 2.08248828196627e-18 MirTarget -0.101052304172802 1.07103513477942e-05 NA NA NA hsa-miR-361-5p ZMAT3 -0.0996867547900457 0.095428250126326 -0.969402662641862 2.08248828196627e-18 MirTarget;miRNATAP -0.422471389194417 1.00126825887328e-10 NA NA NA hsa-miR-362-5p ZMAT3 -0.248676340901797 0.0726089851456282 -0.969402662641862 2.08248828196627e-18 mirMAP -0.164595315254457 3.19719771492237e-09 NA NA NA hsa-miR-374a-3p ZMAT3 0.34122393953022 2.28714105382856e-05 -0.969402662641862 2.08248828196627e-18 MirTarget;miRNATAP -0.164060199257636 0.000673610307903451 NA NA NA hsa-miR-423-5p ZMAT3 -0.0644843381453004 0.462275547464843 -0.969402662641862 2.08248828196627e-18 miRNATAP -0.186085611575209 2.77297570916111e-05 NA NA NA hsa-miR-425-5p ZMAT3 0.719898949241718 6.01079610190374e-08 -0.969402662641862 2.08248828196627e-18 MirTarget -0.118193710191735 4.38313572602468e-05 NA NA NA hsa-miR-429 ZMAT3 2.84108892259147 3.88520896426515e-64 -0.969402662641862 2.08248828196627e-18 MirTarget;miRNATAP -0.189721172084985 1.44816040795263e-19 NA NA NA hsa-miR-454-3p ZMAT3 1.28177672191095 6.84024979484587e-21 -0.969402662641862 2.08248828196627e-18 MirTarget -0.156227247415181 1.66614030974158e-08 NA NA NA hsa-miR-590-3p ZMAT3 1.27438512890514 1.03164723481469e-23 -0.969402662641862 2.08248828196627e-18 mirMAP;miRNATAP -0.150741809108857 1.10819374170232e-06 NA NA NA hsa-miR-590-5p ZMAT3 0.746515607868274 8.51300479640125e-09 -0.969402662641862 2.08248828196627e-18 miRanda -0.103399334057651 0.000492734479726241 NA NA NA hsa-miR-660-5p ZMAT3 0.406910761707485 0.000156175371762464 -0.969402662641862 2.08248828196627e-18 mirMAP -0.301042070335265 1.67560000903716e-17 NA NA NA hsa-miR-671-5p ZMAT3 1.55248047544015 7.7377784125886e-33 -0.969402662641862 2.08248828196627e-18 MirTarget -0.243851194830942 1.86343160449682e-17 NA NA NA hsa-miR-93-5p ZMAT3 1.01788600209314 1.24312921618126e-21 -0.969402662641862 2.08248828196627e-18 mirMAP -0.423921699132773 1.7864005514716e-35 NA NA NA