miRNA gene miRNA_log2FC miRNA_pvalue gene_log2FC gene_pvalue interactions correlation_beta correlation_pvalue PMID evidence outcome cancer hsa-miR-21-5p AKT2 1.51084295924322 1.54551622173259e-34 -0.343608685894869 1.25471482871759e-05 miRNAWalker2_validate -0.246625533117166 4.43585626011661e-19 NA NA NA hsa-miR-330-5p AKT2 0.442231058604099 0.0053308560843808 -0.343608685894869 1.25471482871759e-05 miRanda -0.10429759179516 1.49133299948889e-05 NA NA NA hsa-miR-342-3p AKT2 -0.319677314984262 0.0449813878317147 -0.343608685894869 1.25471482871759e-05 mirMAP -0.124944719707983 1.72780303356483e-07 NA NA NA hsa-miR-106b-5p AKT3 0.648901865047099 8.24870862047885e-09 -0.658799312069129 0.000471762262250455 miRNATAP -0.257549207275002 0.00147770551388523 NA NA NA hsa-miR-107 AKT3 0.241662461253461 0.0170788899505389 -0.658799312069129 0.000471762262250455 PITA;miRanda -0.603074134996255 1.95007791380395e-11 NA NA NA hsa-miR-122-5p AKT3 -1.23907441373955 4.42181244888642e-06 -0.658799312069129 0.000471762262250455 miRNAWalker2_validate;miRTarBase -0.282597613468804 3.13172096832783e-18 24244539 miR 122 regulates tumorigenesis in hepatocellular carcinoma by targeting AKT3; Here we identify AKT3 as a novel and direct target of miR-122; Restoration of miR-122 expression in HCC cell lines decreases AKT3 levels inhibits cell migration and proliferation and induces apoptosis; These anti-tumor phenotypes can be rescued by reconstitution of AKT3 expression indicating the essential role of AKT3 in miR-122 mediated HCC transformation; Our data strongly suggest that miR-122 is a tumor suppressor that targets AKT3 to regulate tumorigenesis in HCCs and a potential therapeutic candidate for liver cancer tumorigenesis;cell migration liver cancer hsa-miR-146b-5p AKT3 0.419719546795449 0.0457375823220155 -0.658799312069129 0.000471762262250455 miRNAWalker2_validate -0.159678144375669 0.000263366078999813 NA NA NA hsa-miR-15a-5p AKT3 0.349521211668244 0.000770735998804736 -0.658799312069129 0.000471762262250455 miRNAWalker2_validate;miRTarBase;miRNATAP -0.265425410812535 0.00291348888530609 NA NA NA hsa-miR-17-3p AKT3 0.410352776142368 0.00422200500748636 -0.658799312069129 0.000471762262250455 miRNATAP -0.350392140268267 3.46133659001702e-08 NA NA NA hsa-miR-17-5p AKT3 0.698861147045461 2.43854106455398e-05 -0.658799312069129 0.000471762262250455 TargetScan;miRNATAP -0.290786763807918 1.04942988053081e-07 NA NA NA hsa-miR-20a-5p AKT3 0.854326758727003 5.04256669663285e-07 -0.658799312069129 0.000471762262250455 miRNATAP -0.294574079686222 2.60181650003526e-08 NA NA NA hsa-miR-32-3p AKT3 0.219109440121582 0.207217051619543 -0.658799312069129 0.000471762262250455 mirMAP -0.225932925933496 3.62088303609835e-05 NA NA NA hsa-miR-33a-3p AKT3 -0.683025859637737 1.01106097756419e-05 -0.658799312069129 0.000471762262250455 mirMAP -0.229716914608906 0.000100074832030455 NA NA NA hsa-miR-362-3p AKT3 0.814007335895913 2.00108618836568e-09 -0.658799312069129 0.000471762262250455 miRanda -0.223301194831745 0.00083465748836002 NA NA NA hsa-miR-362-5p AKT3 0.717921690799312 1.68392734504524e-05 -0.658799312069129 0.000471762262250455 PITA;TargetScan;miRNATAP -0.147261080417749 0.00734435768679755 NA NA NA hsa-miR-374a-5p AKT3 0.016390363747302 0.869784217438621 -0.658799312069129 0.000471762262250455 mirMAP -0.277843537840681 0.00293899073092604 NA NA NA hsa-miR-502-3p AKT3 0.659598854007546 3.01316453996423e-08 -0.658799312069129 0.000471762262250455 miRNATAP -0.256952206617794 0.000795069967308112 NA NA NA hsa-miR-616-5p AKT3 0.150569904638223 0.402836556304524 -0.658799312069129 0.000471762262250455 mirMAP -0.200000357827185 9.73725948226915e-05 NA NA NA hsa-miR-93-5p AKT3 1.39518349670953 2.84167728726712e-25 -0.658799312069129 0.000471762262250455 miRNATAP -0.283992832133781 1.04884093066001e-05 NA NA NA hsa-miR-125a-5p ANGPT2 -0.91219969372382 1.38256246489548e-07 1.15268392731739 7.05586126458027e-10 miRanda -0.163576686828727 0.00200134342077289 NA NA NA hsa-miR-142-5p ANGPT2 -1.44575553250033 1.18797044680592e-13 1.15268392731739 7.05586126458027e-10 MirTarget -0.176235051817787 0.000132150168489542 NA NA NA hsa-miR-145-5p ANGPT2 -1.4795885711477 6.47308361543414e-16 1.15268392731739 7.05586126458027e-10 MirTarget;miRNATAP -0.196630651852303 5.50134414980236e-05 24384875;27570490 miR 145 functions as tumor suppressor and targets two oncogenes ANGPT2 and NEDD9 in renal cell carcinoma; We further validated those miR-145 targets two oncogenes ANGPT2 and NEDD9 in RCC;MiR 145 functions as a tumor suppressor via regulating angiopoietin 2 in pancreatic cancer cells; The direct action of miR-145 on Ang-2 was predicted by TargetScan and confirmed by luciferase report assay; The expression level of miR-145 was significantly lower and the expression levels of Ang-2 mRNA and protein was significantly higher in the more aggressive pancreatic cancer cells MiaPaCa-2 and Panc-1 when compared to that in BxPC3 cells; Overexpression of miR-145 in the BxPC3 MiaPaCa-2 and Panc-1 cells suppressed the cell invasion and colony formation ability and the expression level of Ang-2 protein in MiaPaCa-2 and Panc-1 cells was also suppressed after pre-miR-145 transfection; Intratumoral delivery of miR-145 inhibited the growth of pancreatic cancer xenografts and angiogenesis in vivo and also suppressed the expression level of angiopoietin-2 protein; MiR-145 functions as a tumor suppressor in pancreatic cancer cells by targeting Ang-2 for translation repression and thus suppresses pancreatic cancer cell invasion and growth which suggests that restoring of miR-145 may be a potential therapeutic target for pancreatic cancer ; kidney renal cell cancer;pancreatic cancer hsa-miR-335-3p ANGPT2 -0.275199247483111 0.106629165248783 1.15268392731739 7.05586126458027e-10 mirMAP -0.161794274727677 0.0030395747905161 NA NA NA hsa-miR-429 ANGPT2 -1.40262535827285 6.93982219171912e-05 1.15268392731739 7.05586126458027e-10 miRanda -0.100569047157387 0.00013697862777396 NA NA NA hsa-miR-542-3p ANGPT2 -1.31351015149842 3.64642423288576e-22 1.15268392731739 7.05586126458027e-10 MirTarget;miRanda;miRNATAP -0.356485535003704 3.05034551422058e-08 NA NA NA hsa-miR-744-3p ANGPT2 -0.866026958622846 2.43034431433797e-08 1.15268392731739 7.05586126458027e-10 MirTarget -0.153532830433855 0.00937263305214501 NA NA NA hsa-miR-21-5p ATF2 1.51084295924322 1.54551622173259e-34 -0.239353619837203 0.156190712870845 miRNAWalker2_validate -0.180342957288245 0.00335655702560994 NA NA NA hsa-miR-30b-5p ATF2 -0.54056186708617 2.44688760798341e-05 -0.239353619837203 0.156190712870845 mirMAP -0.223536389725042 0.000452180499441184 NA NA NA hsa-miR-103a-3p BCL2 0.765568928457606 3.05497476509383e-12 -0.34630447089245 0.0249701729768122 miRNAWalker2_validate -0.276206643708183 3.69212207145036e-05 NA NA NA hsa-miR-130b-5p BCL2 0.170599813152408 0.337611586432771 -0.34630447089245 0.0249701729768122 mirMAP -0.1684334796147 6.63359359340854e-05 27364335 The level of microRNA-130b in relationship with the expression of PPARγ VEGF-A BCL-2 and apoptosis were analyzed in 91 lung cancer patient samples using immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick end labeling TUNEL assay on tissue microarrays; In vitro and in vivo miR-130b enrichment associated with down-regulation of PPARγ up-regulation of VEGF-A and BCL-2 and decreased apoptosis lung cancer hsa-miR-148a-3p BCL2 -0.748702855596996 3.93313343865641e-07 -0.34630447089245 0.0249701729768122 miRNAWalker2_validate;miRTarBase -0.309765293468013 4.08540378699783e-10 21455217;23975374 MiR 148a promotes apoptosis by targeting Bcl 2 in colorectal cancer;MiR 148a regulates the growth and apoptosis in pancreatic cancer by targeting CCKBR and Bcl 2; Using western blot and luciferase activity assay CCKBR and Bcl-2 were identified as targets of miR-148a; Moreover we also found that the expression of Bcl-2 lacking in 3'UTR could abrogate the pro-apoptosis function of miR-148a ; colorectal cancer;pancreatic cancer hsa-miR-17-5p BCL2 0.698861147045461 2.43854106455398e-05 -0.34630447089245 0.0249701729768122 miRNAWalker2_validate;miRTarBase -0.3141879354563 9.49080059686353e-13 25435430 Combined overexpression of miR-16 and miR-17 greatly reduced Beclin-1 and Bcl-2 expressions respectively; miR-17 overexpression reduced cytoprotective autophagy by targeting Beclin-1 whereas overexpression of miR-16 potentiated paclitaxel induced apoptotic cell death by inhibiting anti-apoptotic protein Bcl-2 lung cancer hsa-miR-186-5p BCL2 -0.060103391373687 0.535288717081205 -0.34630447089245 0.0249701729768122 mirMAP -0.253682116313365 0.00124603296329075 NA NA NA hsa-miR-192-5p BCL2 -0.50188499512787 0.00344713341196035 -0.34630447089245 0.0249701729768122 miRNAWalker2_validate -0.343159016245154 5.19113742652329e-16 26550150 MicroRNA 192 regulates chemo resistance of lung adenocarcinoma for gemcitabine and cisplatin combined therapy by targeting Bcl 2; In this paper we try to test whether miR-192 regulates chemo-resistance in human carcinoma A549 mice model by targeting Bcl-2; MTT assay real-time RT-PCR western blotting assay were used to investigate miR-192 expression levels cell viability ratio and Bcl-2 protein expression levels; Bcl-2 mRNA and protein expression levels up-regulated in miR-192 inhibitor treated tumor; Bcl-2 is a key regulator for miR-192 related chemotherapy resistance; In this study we demonstrate that miR-192 regulates chemoresistance for gemcitabine and cisplatin combined chemotherapy in human adenocarcinoma lung cancer A549 cells and Bcl-2 is the target of miR-192 drug resistance lung cancer hsa-miR-20a-3p BCL2 -0.318177901603857 0.0467885456739855 -0.34630447089245 0.0249701729768122 mirMAP -0.241984307767668 2.15054512819723e-07 NA NA NA hsa-miR-20a-5p BCL2 0.854326758727003 5.04256669663285e-07 -0.34630447089245 0.0249701729768122 miRNAWalker2_validate;miRTarBase -0.309280985956287 4.00465486003896e-13 NA NA NA hsa-miR-32-3p BCL2 0.219109440121582 0.207217051619543 -0.34630447089245 0.0249701729768122 mirMAP -0.197220723036098 9.60236464107476e-06 NA NA NA hsa-miR-365a-3p BCL2 0.164591326307714 0.15325084963213 -0.34630447089245 0.0249701729768122 miRNAWalker2_validate;miRTarBase -0.325345655719122 6.00535770562877e-07 NA NA NA hsa-miR-455-5p BCL2 -0.270546034117157 0.0581320703577731 -0.34630447089245 0.0249701729768122 mirMAP -0.187920872762485 0.00036775461526632 NA NA NA hsa-miR-590-5p BCL2 -0.103355559980404 0.310027295317092 -0.34630447089245 0.0249701729768122 miRanda -0.288806204300719 0.000105733783342118 NA NA NA hsa-miR-616-5p BCL2 0.150569904638223 0.402836556304524 -0.34630447089245 0.0249701729768122 mirMAP -0.279996287836879 9.62044185440687e-12 NA NA NA hsa-miR-618 BCL2 0.13539895350352 0.517145913114103 -0.34630447089245 0.0249701729768122 mirMAP -0.20993134438608 1.19441927849968e-08 NA NA NA hsa-miR-330-5p BCL2L1 0.442231058604099 0.0053308560843808 0.0225608953357561 0.827445946886033 PITA;miRanda;miRNATAP -0.154730615864034 7.70545597695822e-07 NA NA NA hsa-miR-342-3p BCL2L1 -0.319677314984262 0.0449813878317147 0.0225608953357561 0.827445946886033 PITA;miRanda;miRNATAP -0.123843624743073 7.98408137042515e-05 NA NA NA hsa-miR-342-5p BCL2L1 -0.780720763816797 5.21703298730986e-07 0.0225608953357561 0.827445946886033 miRNATAP -0.106591893041378 0.000835493298714261 NA NA NA hsa-miR-484 BCL2L1 0.0857240033709656 0.453982056282229 0.0225608953357561 0.827445946886033 miRNAWalker2_validate -0.214137045460272 1.02827848229123e-06 NA NA NA hsa-miR-140-5p BCL2L11 -0.220106443070144 0.0140713197101242 -0.226896639061646 0.0688380434490034 miRanda -0.187379481404948 0.0062628077595666 NA NA NA hsa-miR-362-5p BCL2L11 0.717921690799312 1.68392734504524e-05 -0.226896639061646 0.0688380434490034 PITA -0.118938533875452 0.000963923247359095 NA NA NA hsa-miR-107 BDNF 0.241662461253461 0.0170788899505389 0.0509404608382562 0.831886089502745 MirTarget;PITA;miRanda;miRNATAP -0.548625326436466 1.91129941815694e-06 27498977 MicroRNA 107 inhibits tumor growth and metastasis by targeting the BDNF mediated PI3K/AKT pathway in human non small lung cancer; In addition BDNF expression was upregulated and inversely correlated with miR-107 in NSCLC tissues; Enforced overexpression of BDNF effectively reversed the tumor suppressive functions of miR-107 on NSCLC proliferation migration and invasion; miR-107 overexpression or downregulation of BDNF was able to inhibit activation of PI3K/AKT signaling pathway; Taken together our findings present the first evidence that miR-107 could suppress NSCLC metastasis by targeting BDNF and indirectly regulating PI3K/AKT signaling pathway which might lead to a potential therapeutic strategy focusing on miR-107 and BDNF for human NSCLC metastasis lung cancer hsa-miR-148a-5p BDNF -0.767626696027761 1.08359477494797e-06 0.0509404608382562 0.831886089502745 mirMAP -0.25723398567524 0.00044233258998089 NA NA NA hsa-miR-192-3p BDNF -0.6401165114007 0.000266272092403814 0.0509404608382562 0.831886089502745 MirTarget;miRNATAP -0.316265894039516 1.40962674503077e-06 NA NA NA hsa-miR-30d-5p BDNF 0.718283816348023 2.47422074061352e-08 0.0509404608382562 0.831886089502745 miRNATAP -0.295547331188199 0.000945690035537912 NA NA NA hsa-miR-589-5p BDNF 1.18593770505086 1.01781241960531e-22 0.0509404608382562 0.831886089502745 miRNATAP -0.237064825478246 0.0099909566497615 NA NA NA hsa-miR-199a-5p BRCA1 -1.98848462405923 2.0421622713753e-11 0.887514961709891 4.67672259806692e-08 miRanda -0.144408816004622 2.90833289561191e-08 NA NA NA hsa-miR-29b-1-5p BRCA1 -0.538946836809295 0.00102913307251186 0.887514961709891 4.67672259806692e-08 mirMAP -0.178202609524273 0.000255174538062626 NA NA NA hsa-miR-106a-5p CCND1 -0.464229535269236 0.009724944358047 -0.901747452525989 1.22363850855738e-05 MirTarget;miRNATAP -0.262282596122017 2.76721397734651e-06 NA NA NA hsa-miR-106b-5p CCND1 0.648901865047099 8.24870862047885e-09 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.430796355484411 1.07010119372681e-06 NA NA NA hsa-miR-1266-5p CCND1 1.6344248374643 4.09278929749906e-13 -0.901747452525989 1.22363850855738e-05 MirTarget -0.234489057674296 5.64471200142146e-08 NA NA NA hsa-miR-15b-5p CCND1 0.230566941413564 0.0824765001857479 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.544455359613115 3.17021042565567e-13 NA NA NA hsa-miR-16-5p CCND1 -0.398577235179603 0.000101598495180191 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.309506657035983 0.00178218906910022 23991964;22922827;18483394 At the molecular level our results further revealed that cyclin D1 expression was negatively regulated by miR-16;CCND1 has been found to be a target of miR-15a and miR-16-1 through analysis of complementary sequences between microRNAs and CCND1 mRNA; Moreover the transcription of CCND1 is suppressed by miR-15a and miR-16-1 via direct binding to the CCND1 3'-untranslated region 3'-UTR;Truncation in CCND1 mRNA alters miR 16 1 regulation in mantle cell lymphoma; Furthermore we demonstrated that this truncation alters miR-16-1 binding sites and through the use of reporter constructs we were able to show that miR-16-1 regulates CCND1 mRNA expression; This study introduces the role of miR-16-1 in the regulation of CCND1 in MCL ;; bladder cancer;sarcoma;lymphoma hsa-miR-17-5p CCND1 0.698861147045461 2.43854106455398e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;MirTarget;TargetScan;miRNATAP -0.343463418912801 9.97512783987264e-09 26431674 Bioinformatics Prediction and In Vitro Analysis Revealed That miR 17 Targets Cyclin D1 mRNA in Triple Negative Breast Cancer Cells; In this study using bioinformatic analyses miR-17 was selected as it targets the 3'UTR of CCND1 gene with the highest score; After lentiviral transduction of miR-17 to the target cells gene expression analysis showed decreased expression of CCND1 gene breast cancer hsa-miR-186-5p CCND1 -0.060103391373687 0.535288717081205 -0.901747452525989 1.22363850855738e-05 mirMAP -0.315936974513477 0.00286438511586306 NA NA NA hsa-miR-19a-3p CCND1 1.01712596153672 2.57753201450625e-07 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;miRNATAP -0.283475432466515 1.38758823633513e-08 25985117 Moreover miR-19a might play inhibitory roles in HCC malignancy via regulating Cyclin D1 expression liver cancer hsa-miR-19b-1-5p CCND1 -0.277804782598538 0.0783067794722916 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase -0.307712037252966 1.4364183273616e-06 NA NA NA hsa-miR-19b-3p CCND1 0.601279551368824 0.00016685097085251 -0.901747452525989 1.22363850855738e-05 miRNATAP -0.340975400639976 4.6454998539434e-08 NA NA NA hsa-miR-20a-5p CCND1 0.854326758727003 5.04256669663285e-07 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.332506713675419 1.04479750525145e-08 NA NA NA hsa-miR-20b-5p CCND1 0.457668023910379 0.0285865211936156 -0.901747452525989 1.22363850855738e-05 MirTarget;miRNATAP -0.230429652941375 1.603682834845e-06 NA NA NA hsa-miR-340-5p CCND1 -0.00477614531381576 0.968502722773791 -0.901747452525989 1.22363850855738e-05 mirMAP -0.322810275002772 0.000125999869952101 NA NA NA hsa-miR-425-5p CCND1 0.585974239943864 2.23063410931402e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.385144136522737 9.38557217850495e-08 NA NA NA hsa-miR-503-5p CCND1 0.191949282338049 0.268420664548092 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;miRTarBase;MirTarget -0.155187994375291 0.00815133470022587 26047605;23731275 MiR 503 inhibited cell proliferation of human breast cancer cells by suppressing CCND1 expression; Overexpression of miR-503 in breast cancer cell lines reduced cell proliferation through inducing G0/G1 cell cycle arrest by targeting CCND1;MicroRNA 503 suppresses proliferation and cell cycle progression of endometrioid endometrial cancer by negatively regulating cyclin D1; CCND1 has a binding sequence of miR-503 within its 3' untranslated region and was confirmed to be a direct target of miR-503 by the fluorescent reporter assays; Increasing the miR-503 level in EEC cells suppressed cell viability colon formation activity and cell-cycle progression and the inhibited oncogenic phenotypes induced by miR-503 were alleviated by ectopic expression of CCND1 without the untranslated region sequence; Collectively this study suggested that miR-503 plays a tumor-suppressor role by targeting CCND1; Abnormal suppression of miR-503 leads to an increase in the CCND1 level which may promote carcinogenesis and progression of EEC ;progression;tumorigenesis breast cancer;endometrial cancer hsa-miR-589-3p CCND1 1.16846704028095 5.88525574424201e-11 -0.901747452525989 1.22363850855738e-05 MirTarget -0.183119037529565 0.00124255651400669 NA NA NA hsa-miR-616-5p CCND1 0.150569904638223 0.402836556304524 -0.901747452525989 1.22363850855738e-05 mirMAP -0.256039244652268 5.24430962347992e-06 NA NA NA hsa-miR-7-1-3p CCND1 -0.569105143287799 1.85297665041424e-05 -0.901747452525989 1.22363850855738e-05 mirMAP -0.260985322289835 0.000573950021646959 NA NA NA hsa-miR-9-5p CCND1 1.26407994492898 8.53156156439881e-05 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.136746717035529 1.04306605002938e-05 NA NA NA hsa-miR-92a-3p CCND1 0.212391012518195 0.134292268089997 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate -0.407898716180535 8.06773614790968e-09 NA NA NA hsa-miR-93-5p CCND1 1.39518349670953 2.84167728726712e-25 -0.901747452525989 1.22363850855738e-05 miRNAWalker2_validate;MirTarget;miRNATAP -0.335918242999821 2.01286203755089e-06 NA NA NA hsa-miR-942-5p CCND1 0.346451845105263 0.0283255399972342 -0.901747452525989 1.22363850855738e-05 MirTarget -0.245807225430864 0.000123103109119178 NA NA NA hsa-miR-130b-5p CCND2 0.170599813152408 0.337611586432771 0.356451160382274 0.0365641518216489 mirMAP -0.174593683703476 0.000182311423622 NA NA NA hsa-miR-20a-5p CCND2 0.854326758727003 5.04256669663285e-07 0.356451160382274 0.0365641518216489 miRNAWalker2_validate;miRTarBase;miRNATAP -0.156120931730772 0.0012098497067071 NA NA NA hsa-miR-28-5p CCND2 -0.430964830599964 2.67367257252925e-06 0.356451160382274 0.0365641518216489 miRanda -0.482733599739849 7.0104175669049e-08 NA NA NA hsa-miR-33a-3p CCND2 -0.683025859637737 1.01106097756419e-05 0.356451160382274 0.0365641518216489 MirTarget -0.263538375258973 6.17940317063527e-07 NA NA NA hsa-miR-3607-3p CCND2 -2.15518588554493 1.03390728226728e-23 0.356451160382274 0.0365641518216489 mirMAP -0.12362922199284 0.000700039964538526 NA NA NA hsa-miR-378a-3p CCND2 -1.1920055906972 3.23538871733634e-10 0.356451160382274 0.0365641518216489 miRNAWalker2_validate -0.183441253857915 1.70039721501862e-05 NA NA NA hsa-miR-548v CCND2 -0.27258775900243 0.176259917595269 0.356451160382274 0.0365641518216489 MirTarget -0.149460521918892 0.000339780804187451 NA NA NA hsa-miR-616-5p CCND2 0.150569904638223 0.402836556304524 0.356451160382274 0.0365641518216489 mirMAP -0.315443515897926 3.35063962863299e-12 NA NA NA hsa-miR-618 CCND2 0.13539895350352 0.517145913114103 0.356451160382274 0.0365641518216489 mirMAP -0.225947558771367 2.79586020953898e-08 NA NA NA hsa-miR-27b-3p CCND3 -0.823491817819754 9.20935302746823e-14 0.0767102129984094 0.478427267753328 miRNAWalker2_validate -0.236832359235505 2.50262257150465e-07 NA NA NA hsa-miR-320a CCND3 0.325285936317332 0.0221415065838824 0.0767102129984094 0.478427267753328 miRanda -0.118523664136384 0.00134906533817398 NA NA NA hsa-miR-125b-5p CCNE1 -1.35767721102906 1.16564617395164e-18 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate -0.798490415823305 1.91419546517386e-20 NA NA NA hsa-miR-192-5p CCNE1 -0.50188499512787 0.00344713341196035 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate -0.353990461529605 2.35568212305901e-05 NA NA NA hsa-miR-195-5p CCNE1 -1.85907534356434 1.47434041676402e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;MirTarget;miRNATAP -0.296870668934945 3.50489012043558e-05 24402230 Furthermore through qPCR and western blot assays we showed that overexpression of miR-195-5p reduced CCNE1 mRNA and protein levels respectively breast cancer hsa-miR-26a-5p CCNE1 -0.963792031426845 1.83360999387345e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;miRNATAP -0.598523781447039 1.7327978808332e-05 22094936 Cell cycle regulation and CCNE1 and CDC2 were the only significant overlapping pathway and genes differentially expressed between tumors with high and low levels of miR-26a and EZH2 respectively; Low mRNA levels of EZH2 CCNE1 and CDC2 and high levels of miR-26a are associated with favorable outcome on tamoxifen breast cancer hsa-miR-26b-5p CCNE1 -1.10804856399744 4.14176110314282e-22 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;miRNATAP -0.886251741849761 3.83249719633593e-14 NA NA NA hsa-miR-424-5p CCNE1 -2.62653257498508 2.38997974737991e-50 3.04640353472202 4.5986250804531e-28 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.365785229181321 3.5890870773903e-07 NA NA NA hsa-miR-497-5p CCNE1 -1.41371173021948 7.09278946477743e-17 3.04640353472202 4.5986250804531e-28 MirTarget;miRNATAP -0.265241585556134 0.00124914232960422 24112607;25909221;24909281 Western blot assays confirmed that overexpression of miR-497 reduced cyclin E1 protein levels; Inhibited cellular growth suppressed cellular migration and invasion and G1 cell cycle arrest were observed upon overexpression of miR-497 in cells possibly by targeting cyclin E1;The effect of simultaneous overexpression of miR-497 and miR-34a on the inhibition of cell proliferation colony formation and tumor growth and the downregulation of cyclin E1 was stronger than the effect of each miRNA alone; The synergistic actions of miR-497 and miR-34a partly correlated with cyclin E1 levels; These results indicate cyclin E1 is downregulated by both miR-497 and miR-34a which synergistically retard the growth of human lung cancer cells;miR 497 suppresses proliferation of human cervical carcinoma HeLa cells by targeting cyclin E1; Furthermore the target effect of miR-497 on the CCNE1 was identified by dual-luciferase reporter assay system qRT-PCR and Western blotting; Over-expressed miR-497 in HeLa cells could suppress cell proliferation by targeting CCNE1 ;; breast cancer;lung cancer;cervical and endocervical cancer hsa-let-7b-3p CCNE2 -1.21979213547786 5.84575581584603e-17 2.01834184083791 5.42527234853282e-21 mirMAP -0.268569886454063 0.000212358107274223 NA NA NA hsa-miR-126-3p CCNE2 -0.652349350198172 9.37876280344772e-09 2.01834184083791 5.42527234853282e-21 miRNAWalker2_validate -0.385200741422764 5.31716743581417e-05 NA NA NA hsa-miR-26a-5p CCNE2 -0.963792031426845 1.83360999387345e-22 2.01834184083791 5.42527234853282e-21 miRNAWalker2_validate;miRTarBase;miRNATAP -0.488121351666366 3.8244940657208e-06 24116110;21901171 The loss of miR 26a mediated post transcriptional regulation of cyclin E2 in pancreatic cancer cell proliferation and decreased patient survival; The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest inhibited cell proliferation and decreased tumor growth which was associated with cyclin E2 downregulation;We also show that enforced expression of miR-26a in AML cells is able to inhibit cell cycle progression by downregulating cyclin E2 expression poor survival;progression pancreatic cancer;acute myeloid leukemia hsa-miR-26b-5p CCNE2 -1.10804856399744 4.14176110314282e-22 2.01834184083791 5.42527234853282e-21 miRNATAP -0.579195472860141 1.12131120131545e-10 NA NA NA hsa-miR-30a-5p CCNE2 -0.626257932887855 0.000108198196973765 2.01834184083791 5.42527234853282e-21 miRNATAP -0.356376866932366 9.6729026873686e-08 NA NA NA hsa-miR-122-5p CDK4 -1.23907441373955 4.42181244888642e-06 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate -0.116994239033144 4.65868213756134e-11 NA NA NA hsa-miR-145-5p CDK4 -1.4795885711477 6.47308361543414e-16 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate;miRTarBase -0.153089865179612 2.59222750442516e-09 21092188 Furthermore we found that CDK4 was regulated by miR-145 in cell cycle control lung squamous cell cancer hsa-miR-193b-3p CDK4 -0.166697207281748 0.272022543069482 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate -0.152018771778108 2.97527104247713e-06 NA NA NA hsa-miR-195-5p CDK4 -1.85907534356434 1.47434041676402e-22 0.667631464196621 2.00897661385551e-11 miRNAWalker2_validate;miRTarBase -0.181976803431634 3.34979886785679e-14 NA NA NA hsa-let-7a-3p CDK6 -0.568717557554068 1.00947202557819e-07 -0.306731100792328 0.220566158555725 miRNATAP -0.341190533180457 0.00261304127571301 NA NA NA hsa-let-7b-5p CDK6 -0.959013717270803 5.95127281258643e-11 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.21783266130525 0.0075638804735268 NA NA NA hsa-miR-106a-5p CDK6 -0.464229535269236 0.009724944358047 -0.306731100792328 0.220566158555725 mirMAP -0.278977768977315 3.5360030094147e-05 NA NA NA hsa-miR-106b-5p CDK6 0.648901865047099 8.24870862047885e-09 -0.306731100792328 0.220566158555725 mirMAP -0.387039073753013 0.000291052086223802 NA NA NA hsa-miR-141-3p CDK6 -0.354316381352484 0.256996894288799 -0.306731100792328 0.220566158555725 TargetScan;miRNATAP -0.140574578703219 0.000314373241862064 NA NA NA hsa-miR-148b-3p CDK6 0.266862251671911 0.00185271302045935 -0.306731100792328 0.220566158555725 mirMAP -0.757154003587567 1.00024492805767e-07 NA NA NA hsa-miR-16-5p CDK6 -0.398577235179603 0.000101598495180191 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.498098719613092 2.63556306703195e-05 NA NA NA hsa-miR-17-5p CDK6 0.698861147045461 2.43854106455398e-05 -0.306731100792328 0.220566158555725 TargetScan;mirMAP -0.333266464458022 4.3415707047215e-06 NA NA NA hsa-miR-182-5p CDK6 1.96601340710222 4.39326175603633e-09 -0.306731100792328 0.220566158555725 mirMAP -0.10653370302744 0.0027913600827753 NA NA NA hsa-miR-195-5p CDK6 -1.85907534356434 1.47434041676402e-22 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase -0.257207165713251 2.04308440041189e-05 23333942 Expression of cyclin-dependent kinase 6 and vascular endothelial growth factor was down-regulated by exogenous miR-195 and miR-378 respectively gastric cancer hsa-miR-200c-3p CDK6 -0.104675615392797 0.716957669484981 -0.306731100792328 0.220566158555725 mirMAP -0.120071361082355 0.00446253530887124 NA NA NA hsa-miR-20a-5p CDK6 0.854326758727003 5.04256669663285e-07 -0.306731100792328 0.220566158555725 mirMAP -0.336108073900107 1.71022894952879e-06 NA NA NA hsa-miR-20b-5p CDK6 0.457668023910379 0.0285865211936156 -0.306731100792328 0.220566158555725 mirMAP -0.209722611209641 0.000304623744169473 26166554 The transfection of miR-20b into EJ cells induced G1 phase cell cycle arrest via the decreased expression of cyclin D1 CDK2 and CDK6 without affecting another G1 phase cell cycle regulator cyclin E bladder cancer hsa-miR-217 CDK6 1.05797297540381 0.0401026692682422 -0.306731100792328 0.220566158555725 mirMAP -0.175237317848738 1.85593116207955e-14 NA NA NA hsa-miR-224-3p CDK6 1.40770116795301 1.31426607144612e-08 -0.306731100792328 0.220566158555725 mirMAP -0.144127925871375 0.00324265854216547 NA NA NA hsa-miR-26a-5p CDK6 -0.963792031426845 1.83360999387345e-22 -0.306731100792328 0.220566158555725 miRNAWalker2_validate;miRTarBase;miRNATAP -0.450700287383469 0.000126142178652791 26314438 Maxvision immunohistochemistry technique was used to detect the expression level of CDK6 and miR-26a in tissue of 20 ENKTCL cases 10 cases of proliferative lymphadenitis and 10 samples of normal lymph node respectively; The possible role of miR-26a and its target gene CDK6 in genesis and development of ENKTCL were analyzed according to the clinical features of ENKTCL patients; Correlation analysis showed that there was significant negative correlation between miR-26a expression and CDK6 expression r = -0.54 P = 0.04 lymphoma hsa-miR-338-3p CDK6 0.543135607704581 0.00461167747143826 -0.306731100792328 0.220566158555725 mirMAP -0.192008504458263 0.00245323065472347 NA NA NA hsa-miR-340-5p CDK6 -0.00477614531381576 0.968502722773791 -0.306731100792328 0.220566158555725 mirMAP -0.591488535627004 3.30562522942768e-09 NA NA NA hsa-miR-425-5p CDK6 0.585974239943864 2.23063410931402e-05 -0.306731100792328 0.220566158555725 mirMAP -0.371054247671967 2.11644953421021e-05 NA NA NA hsa-miR-452-5p CDK6 1.91544498799508 1.81995982853313e-14 -0.306731100792328 0.220566158555725 mirMAP -0.148489463562499 0.00161659837456088 NA NA NA hsa-miR-497-5p CDK6 -1.41371173021948 7.09278946477743e-17 -0.306731100792328 0.220566158555725 miRNATAP -0.339765827186355 7.37877046693881e-07 NA NA NA hsa-miR-502-3p CDK6 0.659598854007546 3.01316453996423e-08 -0.306731100792328 0.220566158555725 PITA;miRNATAP -0.485378420730533 1.35374384457666e-06 NA NA NA hsa-miR-616-5p CDK6 0.150569904638223 0.402836556304524 -0.306731100792328 0.220566158555725 mirMAP -0.218127986999019 0.00133578296465318 NA NA NA hsa-miR-885-5p CDK6 -0.940994379567566 0.00118729865914986 -0.306731100792328 0.220566158555725 mirMAP -0.312653333627933 1.15118921616921e-14 NA NA NA hsa-miR-92a-3p CDK6 0.212391012518195 0.134292268089997 -0.306731100792328 0.220566158555725 miRNATAP -0.478355192378327 1.89776806005731e-08 NA NA NA hsa-miR-106b-5p CDKN1A 0.648901865047099 8.24870862047885e-09 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.437829469030798 7.84136969105116e-08 NA NA NA hsa-miR-146b-5p CDKN1A 0.419719546795449 0.0457375823220155 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate -0.153153076910721 0.000589578943698456 27602131 During the search for potential targets of miR-146b in ATC p21 also known as p21Waf1/Cip1 or CDKN1A was noted for its role in cell cycle progression and tumor pathogenesis progression thyroid cancer hsa-miR-17-5p CDKN1A 0.698861147045461 2.43854106455398e-05 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.29756314131737 8.83846469129569e-08 26482648;24989082 The low expressions of miR-17 and miR-92 families can maintain cisplatin resistance through the regulation of CDKN1A and RAD21;According to PicTar and Miranda algorithms which predicted CDKN1A p21 as a putative target of miR-17 a luciferase assay was performed and revealed that miR-17 directly targets the 3'-UTR of p21 mRNA drug resistance; lung squamous cell cancer;sarcoma hsa-miR-20a-5p CDKN1A 0.854326758727003 5.04256669663285e-07 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.281025701374886 1.92150921220327e-07 26012475 Using the poorly tumorigenic and TGF-β-sensitive FET cell line that expresses low miR-20a levels we first confirmed that miR-20a downmodulated CDKN1A expression both at mRNA and protein level through direct binding to its 3'-UTR; Moreover besides modulating CDKN1A miR-20a blocked TGF-β-induced transactivation of its promoter without affecting the post-receptor activation of Smad3/4 effectors directly; Finally miR-20a abrogated the TGF-β-mediated c-Myc repression a direct inhibitor of the CDKN1A promoter activation most likely by reducing the expression of specific MYC-regulating genes from the Smad/E2F-based core repressor complex colon cancer hsa-miR-423-3p CDKN1A 0.302157659154355 0.000669907339064765 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase -0.424628898441296 6.37065354057174e-05 NA NA NA hsa-miR-423-5p CDKN1A 0.698807775906485 1.02195196334612e-11 -0.769088197669944 5.80039048047741e-05 MirTarget -0.370602271693157 3.44327865138196e-05 NA NA NA hsa-miR-503-5p CDKN1A 0.191949282338049 0.268420664548092 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase -0.140430121948101 0.00973159792553366 NA NA NA hsa-miR-93-5p CDKN1A 1.39518349670953 2.84167728726712e-25 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.446611351561038 4.29858025785308e-12 25633810 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC liver cancer hsa-miR-942-5p CDKN1A 0.346451845105263 0.0283255399972342 -0.769088197669944 5.80039048047741e-05 miRNAWalker2_validate -0.264158154736877 7.79693170645745e-06 NA NA NA hsa-miR-181a-5p CDKN1B 0.250113435902954 0.0551933249319293 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase -0.134068475887496 1.13890102336643e-05 NA NA NA hsa-miR-221-3p CDKN1B 1.12244756358612 3.48370308238012e-13 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.153438831523776 5.56071406720925e-10 20146005;23637992;19953484;23939688;19126397;23967190;17569667;22992757;17721077;20461750 Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27Kip1 p21WAF1/Cip1and TGF-β1 by in situ hybridization and immunohistochemistry respectively; Real time qRT-PCR was used to investigate miR-221 and p27Kip1 transcripts in different clinical stages; In result miR-221 and TGF-β1 are frequently up-regulated in HCC while p27Kip1 and p21WAF1/Cip1 proteins are frequently down-regulated; In conclusion miR-221 is important in tumorigenesis of HCC possibly by specifically down-regulating p27Kip1 a cell-cycle inhibitor;miR-221 knockdown not only blocked cell cycle progression induced cell apoptosis and inhibited cell proliferation in-vitro but it also inhibited in-vivo tumor growth by targeting p27kip1;Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;A Slug/miR-221 network has been suggested linking miR-221 activity with the downregulation of a Slug repressor leading to Slug/miR-221 upregulation and p27Kip1 downregulation; Interference with this process can be achieved using antisense miRNA antagomiR molecules targeting miR-221 inducing the downregulation of Slug and the upregulation of p27Kip1;Moreover a series of functional assays demonstrated that mir-221 could directly inhibit cKit p27Kip1 and possibly other pivotal proteins in melanoma;Additionally the PDGF-dependent increase in cell proliferation appears to be mediated by inhibition of a specific target of miR-221 and down-regulation of p27Kip1;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;Peptide nucleic acids targeting miR 221 modulate p27Kip1 expression in breast cancer MDA MB 231 cells; Targeting miR-221 by PNA resulted in i lowering of the hybridization levels of miR-221 measured by RT-qPCR ii upregulation of p27Kip1 gene expression measured by RT-qPCR and western blot analysis;Antagonism of either microRNA 221 or 222 in glioblastoma cells also caused an increase in p27Kip1 levels and enhanced expression of the luciferase reporter gene fused to the p27Kip1 3'UTR;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression staging;tumorigenesis;progression;;;;;;;;poor survival liver cancer;breast cancer;glioblastoma;breast cancer;melanoma;pancreatic cancer;prostate cancer;breast cancer;glioblastoma;ovarian cancer hsa-miR-222-3p CDKN1B 1.08574156066353 1.76407192348131e-11 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.140430207935426 3.56247154847449e-09 19953484;26912358;24895988;24137356;17569667;27282281;20461750 Based on bioinformatic analysis we found that the seed sequences of miR-221 and miR-222 coincide with each other and p27kip1 is a target for miRNA-221/222;Besides microvesicle marker characterization we evidenced that miR-222 exosomal expression mostly reflected its abundance in the cells of origin correctly paralleled by repression of its target genes such as p27Kip1 and induction of the PI3K/AKT pathway thus confirming its functional implication in cancer;MiR-222 plays an important role in the tumorigenesis of CC possibly by specifically down-regulating p27Kip1 and PTEN;miR 222 is upregulated in epithelial ovarian cancer and promotes cell proliferation by downregulating P27kip1; miR-222 upregulation induced an enhancement of ovarian cancer cell proliferation potential possibly by downregulating its target P27Kip1; A bioinformatic analysis showed that the 3'-UTR of the P27Kip1 mRNA contained a highly-conserved putative miR-222 binding site; Luciferase reporter assays demonstrated that P27Kip1 was a direct target of miR-222; Consistently there was an inverse correlation between the P27Kip1 and miR-222 expression levels in the ovarian cancer cell lines and tissues;miR 221 and miR 222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27Kip1; In all cell lines tested we show an inverse relationship between the expression of miR-221 and miR-222 and the cell cycle inhibitor p27Kip1; Consistently miR-221 and miR-222 knock-down through antisense LNA oligonucleotides increases p27Kip1 in PC3 cells and strongly reduces their clonogenicity in vitro;miR 222 confers the resistance of breast cancer cells to Adriamycin through suppression of p27kip1 expression; Immunofluorescence showed that miR-222 altered the subcellular location of p27kip1 in nucleus; The results showed that downregulation of miR-222 in MCF-7/Adr increased sensitivity to Adr and Adr-induced apoptosis and arrested the cells in G1 phase accompanied by more expressions of p27kip1 especially in nucleus; Taken together the results found that miR-222 induced Adr-resistance at least in part via suppressing p27kip1 expression and altering its subcellular localization and miR-222 inhibitors could reverse Adr-resistance of breast cancer cells;MiR 221 and MiR 222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B CDKNIC and overall survival; miR-221 and miR-222 negatively regulate expression of CDKN1B p27 and CDKN1C p57 two cell cycle regulators expressed in ovarian surface epithelium and down-regulated in ovarian carcinomas; In contrast CDKN1B expression was not associated with miR-221 or miR-222 expression ;;tumorigenesis;;;drug resistance;poor survival glioblastoma;melanoma;cervical and endocervical cancer;ovarian cancer;prostate cancer;breast cancer;ovarian cancer hsa-miR-24-3p CDKN1B -0.257825594021966 0.00689742600215061 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate;miRNATAP -0.125575697425242 0.00293958904599202 26847530;26044523 The biological significance of miR-24 expression in prostate cancer cells was assessed by a series of in vitro bioassays and the effect on proposed targets p27 CDKN1B and p16 CDK2NA was investigated;With the bioinformatic method we further identified that p27Kip1 is a direct target of miR-24-3p and its protein level was negatively regulated by miR-24-3p ; prostate cancer;breast cancer hsa-miR-98-5p CDKN1B -0.0487189107419432 0.715911066732548 -0.231452033928625 0.00482071055692889 miRNAWalker2_validate -0.150589087046664 3.98220273478164e-07 NA NA NA hsa-miR-199a-3p CHAD -2.3279328685682 1.04468066198593e-17 -0.971936204452971 0.000334764618282157 MirTarget;miRNATAP -0.173935024249055 0.000195324497109954 NA NA NA hsa-miR-199b-3p CHAD -2.33226605401203 9.51887435273314e-18 -0.971936204452971 0.000334764618282157 MirTarget -0.173511442303337 0.000200423270036301 NA NA NA hsa-miR-222-5p CHAD 0.125716360553359 0.487423909991138 -0.971936204452971 0.000334764618282157 MirTarget -0.26343390233107 0.000341452540988748 NA NA NA hsa-miR-330-3p CHAD -0.332364259424489 0.0316051913061459 -0.971936204452971 0.000334764618282157 miRNATAP -0.447100757418491 2.80653318469262e-07 NA NA NA hsa-miR-369-3p CHAD -0.680843625018478 0.0155063508282041 -0.971936204452971 0.000334764618282157 MirTarget -0.138608609327556 0.00326595687687768 NA NA NA hsa-miR-493-3p CHAD -0.788022807036478 0.00722886624742207 -0.971936204452971 0.000334764618282157 MirTarget -0.12399184566229 0.00678010748530443 NA NA NA hsa-miR-15a-5p CHUK 0.349521211668244 0.000770735998804736 -0.19932080126218 0.00863163957240649 miRNAWalker2_validate;miRTarBase;MirTarget -0.122536038974783 0.000565424625908096 NA NA NA hsa-miR-15b-5p CHUK 0.230566941413564 0.0824765001857479 -0.19932080126218 0.00863163957240649 MirTarget -0.118017359725558 2.06238744655946e-05 NA NA NA hsa-miR-16-5p CHUK -0.398577235179603 0.000101598495180191 -0.19932080126218 0.00863163957240649 miRNAWalker2_validate;miRTarBase;MirTarget -0.150607357209835 2.62400382273556e-05 NA NA NA hsa-miR-23a-3p CHUK -0.178875974460574 0.135978273540604 -0.19932080126218 0.00863163957240649 MirTarget -0.100438227795724 0.00114468004476388 NA NA NA hsa-miR-339-5p CHUK 0.280589919878422 0.0355660282647215 -0.19932080126218 0.00863163957240649 miRanda -0.12221351609504 8.92727609693918e-06 NA NA NA hsa-miR-590-5p CHUK -0.103355559980404 0.310027295317092 -0.19932080126218 0.00863163957240649 miRanda -0.11766978557823 0.00128889069327197 NA NA NA hsa-miR-107 COL1A1 0.241662461253461 0.0170788899505389 0.826702197173396 0.00648724292232758 PITA;miRanda -1.03604844961437 6.189177258338e-13 NA NA NA hsa-miR-193a-5p COL1A1 -0.472805720224262 1.24962968905481e-05 0.826702197173396 0.00648724292232758 PITA;TargetScan;miRNATAP -0.631963739355247 3.30700752875273e-06 NA NA NA hsa-miR-20a-5p COL1A1 0.854326758727003 5.04256669663285e-07 0.826702197173396 0.00648724292232758 mirMAP -0.233621814946948 0.00679703428396713 NA NA NA hsa-miR-23b-5p COL1A1 -1.05289229952702 7.22203199173865e-13 0.826702197173396 0.00648724292232758 mirMAP -0.273119936989386 0.00677641533350269 NA NA NA hsa-miR-30b-3p COL1A1 -0.439724365151373 0.000947180503583586 0.826702197173396 0.00648724292232758 miRNATAP -0.407003170758284 0.000252249930807467 NA NA NA hsa-miR-30e-3p COL1A1 -1.20845647373031 3.10263633528203e-25 0.826702197173396 0.00648724292232758 mirMAP -0.436158045118392 0.000287836135145749 NA NA NA hsa-miR-532-3p COL1A1 0.297601533259956 0.014632205884815 0.826702197173396 0.00648724292232758 PITA;miRNATAP -0.459574968886081 0.000161776311559917 NA NA NA hsa-miR-616-5p COL1A1 0.150569904638223 0.402836556304524 0.826702197173396 0.00648724292232758 mirMAP;miRNATAP -0.460861872336035 1.70679592021185e-08 NA NA NA hsa-miR-26b-5p COL1A2 -1.10804856399744 4.14176110314282e-22 0.58018696776109 0.0267399288126224 MirTarget;miRNATAP -0.336616912489242 0.0015435755362445 NA NA NA hsa-miR-30d-3p COL1A2 -0.120562286038759 0.329549534782125 0.58018696776109 0.0267399288126224 MirTarget -0.270934115842966 0.00928784242471402 NA NA NA hsa-miR-30e-3p COL1A2 -1.20845647373031 3.10263633528203e-25 0.58018696776109 0.0267399288126224 MirTarget -0.281145660318855 0.00682336575635351 NA NA NA hsa-miR-32-3p COL1A2 0.219109440121582 0.207217051619543 0.58018696776109 0.0267399288126224 miRNATAP -0.37279076503956 7.6563506038813e-07 NA NA NA hsa-miR-32-5p COL1A2 0.0806126674664016 0.548984992497432 0.58018696776109 0.0267399288126224 MirTarget;miRNATAP -0.613101144317773 5.76821580215829e-11 NA NA NA hsa-miR-33a-3p COL1A2 -0.683025859637737 1.01106097756419e-05 0.58018696776109 0.0267399288126224 MirTarget -0.501154698930155 5.09589827659522e-10 NA NA NA hsa-miR-7-1-3p COL1A2 -0.569105143287799 1.85297665041424e-05 0.58018696776109 0.0267399288126224 mirMAP -0.264273750473028 0.00576750957648269 NA NA NA hsa-miR-92a-3p COL1A2 0.212391012518195 0.134292268089997 0.58018696776109 0.0267399288126224 MirTarget;miRNATAP -0.312053884384404 0.000544719042919194 NA NA NA hsa-miR-152-3p COL2A1 -0.959123731390966 3.24700662487287e-10 2.31956756673828 0.000172146394351314 MirTarget -0.687177847226081 0.000407041648666243 NA NA NA hsa-miR-29a-3p COL2A1 -0.856762511178136 4.65330336378459e-12 2.31956756673828 0.000172146394351314 MirTarget;miRNATAP -1.86897107051162 6.99911894448077e-16 NA NA NA hsa-miR-29b-3p COL2A1 -0.348885528720517 0.0121441341291285 2.31956756673828 0.000172146394351314 MirTarget;miRNATAP -0.95094174795995 1.15421046001363e-05 NA NA NA hsa-miR-29c-3p COL2A1 -1.4367517608807 1.61767774905781e-21 2.31956756673828 0.000172146394351314 MirTarget;miRNATAP -1.27860412529072 8.10710043682058e-12 NA NA NA hsa-miR-148a-3p COL4A1 -0.748702855596996 3.93313343865641e-07 1.64595918385092 3.29766696573805e-22 miRNATAP -0.506696445918838 1.45523526380931e-19 NA NA NA hsa-miR-193b-3p COL4A1 -0.166697207281748 0.272022543069482 1.64595918385092 3.29766696573805e-22 miRNAWalker2_validate -0.218530092845592 0.000144043698376859 NA NA NA hsa-miR-29c-3p COL4A1 -1.4367517608807 1.61767774905781e-21 1.64595918385092 3.29766696573805e-22 miRNAWalker2_validate;miRTarBase;miRNATAP -0.17365265771026 0.00160253083531795 NA NA NA hsa-miR-590-3p COL4A1 -0.473167498047808 1.76652934637227e-05 1.64595918385092 3.29766696573805e-22 miRanda -0.232842070891326 0.00321669636636402 NA NA NA hsa-miR-590-5p COL4A1 -0.103355559980404 0.310027295317092 1.64595918385092 3.29766696573805e-22 miRanda;miRNATAP -0.403715680850443 2.64148852714533e-06 NA NA NA hsa-miR-99a-5p COL4A1 -1.51481656384839 3.93230057259529e-14 1.64595918385092 3.29766696573805e-22 miRNAWalker2_validate -0.194902289048293 3.21789355543509e-06 NA NA NA hsa-miR-26b-5p COL4A2 -1.10804856399744 4.14176110314282e-22 1.44307871157488 7.7234079265155e-18 miRNAWalker2_validate -0.427711605173566 8.4579800793199e-10 NA NA NA hsa-miR-29c-3p COL4A2 -1.4367517608807 1.61767774905781e-21 1.44307871157488 7.7234079265155e-18 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.147672102686362 0.00605514035830943 NA NA NA hsa-miR-30c-1-3p COL4A2 -1.39373825444537 6.39912952911454e-17 1.44307871157488 7.7234079265155e-18 MirTarget -0.299314528692518 1.02267945249468e-09 NA NA NA hsa-miR-107 COL4A3 0.241662461253461 0.0170788899505389 -0.92673315541801 0.00622463110440454 miRanda -0.903152068242314 2.78749342267172e-08 NA NA NA hsa-miR-17-5p COL4A3 0.698861147045461 2.43854106455398e-05 -0.92673315541801 0.00622463110440454 MirTarget;TargetScan;miRNATAP -0.287999625105642 0.00371613172922971 NA NA NA hsa-miR-20a-5p COL4A3 0.854326758727003 5.04256669663285e-07 -0.92673315541801 0.00622463110440454 MirTarget;miRNATAP -0.275914518317586 0.00414348195590725 NA NA NA hsa-miR-374a-5p COL4A3 0.016390363747302 0.869784217438621 -0.92673315541801 0.00622463110440454 MirTarget -0.519609729384737 0.00192416477237929 NA NA NA hsa-miR-374b-5p COL4A3 -0.307538028546424 0.0030079617545972 -0.92673315541801 0.00622463110440454 MirTarget -0.436820503736535 0.00659195534688853 NA NA NA hsa-miR-93-5p COL4A3 1.39518349670953 2.84167728726712e-25 -0.92673315541801 0.00622463110440454 MirTarget;miRNATAP -0.389886875891421 0.000790866332079855 NA NA NA hsa-miR-107 COL4A4 0.241662461253461 0.0170788899505389 -1.21297499956657 3.63510226190771e-06 miRanda -0.59211828231812 3.37972306193379e-06 NA NA NA hsa-miR-30d-3p COL4A4 -0.120562286038759 0.329549534782125 -1.21297499956657 3.63510226190771e-06 MirTarget -0.361582241495628 0.000564725814150975 NA NA NA hsa-miR-361-5p COL4A4 0.233202959858696 0.00961572812255327 -1.21297499956657 3.63510226190771e-06 MirTarget;miRanda -0.642410170202205 8.09722110245279e-06 NA NA NA hsa-miR-532-3p COL4A4 0.297601533259956 0.014632205884815 -1.21297499956657 3.63510226190771e-06 miRNATAP -0.356203500689371 0.000788983951195529 NA NA NA hsa-miR-589-3p COL4A4 1.16846704028095 5.88525574424201e-11 -1.21297499956657 3.63510226190771e-06 mirMAP -0.189790713873612 0.00867611857279342 NA NA NA hsa-let-7g-5p COL4A5 -0.461294133285527 2.17210114729901e-05 0.0635599577482339 0.881381174188716 miRNATAP -0.552138080738768 0.00378312378832122 NA NA NA hsa-miR-29a-3p COL4A5 -0.856762511178136 4.65330336378459e-12 0.0635599577482339 0.881381174188716 miRNATAP -0.456561761721898 0.00533571331578358 NA NA NA hsa-miR-455-5p COL4A5 -0.270546034117157 0.0581320703577731 0.0635599577482339 0.881381174188716 miRanda -0.384902949893891 0.00826052024568226 NA NA NA hsa-miR-146b-5p COL6A3 0.419719546795449 0.0457375823220155 0.169833671007575 0.432293683806163 miRanda -0.130033763284282 0.00943159485447041 NA NA NA hsa-miR-28-5p COL6A3 -0.430964830599964 2.67367257252925e-06 0.169833671007575 0.432293683806163 miRanda -0.367874691394583 0.00133476304471087 NA NA NA hsa-miR-103a-2-5p COL6A6 1.17085533927423 5.31418969120877e-09 -4.73538512673886 1.28847853486663e-55 mirMAP -0.252017029051465 0.003732445623638 NA NA NA hsa-miR-501-5p COL6A6 1.15231083477882 2.98906150004088e-10 -4.73538512673886 1.28847853486663e-55 mirMAP -0.399485266876571 8.694918835706e-06 NA NA NA hsa-miR-532-3p COL6A6 0.297601533259956 0.014632205884815 -4.73538512673886 1.28847853486663e-55 MirTarget -0.636102466311464 3.99010132695655e-06 NA NA NA hsa-miR-616-5p COL6A6 0.150569904638223 0.402836556304524 -4.73538512673886 1.28847853486663e-55 mirMAP -0.276844083417891 0.0032935214386195 NA NA NA hsa-let-7b-3p COL9A1 -1.21979213547786 5.84575581584603e-17 2.78909880839119 5.63828792375664e-23 mirMAP -0.351502187097791 0.000250199300049881 NA NA NA hsa-let-7f-1-3p COL9A1 -0.704017923073338 7.84883628944999e-07 2.78909880839119 5.63828792375664e-23 mirMAP -0.324133206336437 0.00155532499127613 NA NA NA hsa-miR-29a-3p COL9A1 -0.856762511178136 4.65330336378459e-12 2.78909880839119 5.63828792375664e-23 miRNATAP -0.461604892015553 5.53828210712123e-05 NA NA NA hsa-miR-29c-3p COL9A1 -1.4367517608807 1.61767774905781e-21 2.78909880839119 5.63828792375664e-23 miRNATAP -0.435276338625617 1.77750960156697e-06 NA NA NA hsa-miR-590-3p COL9A1 -0.473167498047808 1.76652934637227e-05 2.78909880839119 5.63828792375664e-23 miRanda -0.438513536053524 0.000866837237220512 NA NA NA hsa-miR-7-1-3p COL9A1 -0.569105143287799 1.85297665041424e-05 2.78909880839119 5.63828792375664e-23 mirMAP -0.413105486578975 0.00014245691561765 NA NA NA hsa-miR-22-3p CREB1 -0.628561621952407 1.33755558015523e-09 -0.00947108171252431 0.883800737430634 MirTarget -0.207046355261965 1.09327700723103e-12 NA NA NA hsa-miR-107 CREB3 0.241662461253461 0.0170788899505389 0.198317768133849 0.0179354122530865 miRanda -0.123995835564553 0.00219401862702445 NA NA NA hsa-miR-1271-5p CREB3L2 -1.00001044718608 1.30308965099272e-09 0.504185492103248 2.44907819598032e-07 MirTarget -0.101517857394041 0.000427355689580642 NA NA NA hsa-miR-93-3p CREB3L3 0.402821129107592 0.00131324842735588 -1.30003732784553 1.11329047056982e-05 mirMAP -0.302525304679396 0.0093345004910047 NA NA NA hsa-miR-107 CREB5 0.241662461253461 0.0170788899505389 0.00174118159445535 0.993131455555257 miRNATAP -0.595255759254272 6.03560855072754e-10 NA NA NA hsa-miR-130b-3p CREB5 0.687190305027546 0.000109068658562255 0.00174118159445535 0.993131455555257 miRNATAP -0.27564316508303 4.20533776574786e-07 NA NA NA hsa-miR-204-5p CREB5 -0.537962150012103 0.0330895493358849 0.00174118159445535 0.993131455555257 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.102281351900569 0.00866640620939317 NA NA NA hsa-miR-32-5p CREB5 0.0806126674664016 0.548984992497432 0.00174118159445535 0.993131455555257 miRNAWalker2_validate -0.190840057611096 0.00953603626614589 NA NA NA hsa-miR-33a-3p CREB5 -0.683025859637737 1.01106097756419e-05 0.00174118159445535 0.993131455555257 miRNATAP -0.197044135890035 0.00180186218936308 NA NA NA hsa-miR-362-5p CREB5 0.717921690799312 1.68392734504524e-05 0.00174118159445535 0.993131455555257 PITA -0.155888821968308 0.00775377113263217 NA NA NA hsa-miR-365a-3p CREB5 0.164591326307714 0.15325084963213 0.00174118159445535 0.993131455555257 MirTarget -0.338243003110366 7.8069347393161e-05 NA NA NA hsa-miR-532-3p CREB5 0.297601533259956 0.014632205884815 0.00174118159445535 0.993131455555257 miRNATAP -0.317565470573069 8.27667566691462e-05 NA NA NA hsa-miR-548b-3p CREB5 -0.0203576084717134 0.930819503166956 0.00174118159445535 0.993131455555257 PITA -0.214407981842792 3.57141654423001e-07 NA NA NA hsa-miR-576-5p CREB5 -0.377452008285847 0.00470735657343984 0.00174118159445535 0.993131455555257 PITA;mirMAP -0.238700205564156 0.00119245252352199 NA NA NA hsa-miR-616-5p CREB5 0.150569904638223 0.402836556304524 0.00174118159445535 0.993131455555257 MirTarget;mirMAP -0.241393018835134 9.6475172843451e-06 NA NA NA hsa-miR-107 CSF1 0.241662461253461 0.0170788899505389 -0.5853277886696 0.00153229238014141 miRanda -0.285515676631282 0.00148342702036097 NA NA NA hsa-miR-148a-3p CSF1 -0.748702855596996 3.93313343865641e-07 -0.5853277886696 0.00153229238014141 miRNATAP -0.256768312227477 2.00461329540934e-05 NA NA NA hsa-miR-17-5p CSF1 0.698861147045461 2.43854106455398e-05 -0.5853277886696 0.00153229238014141 TargetScan;miRNATAP -0.242663156767433 6.44086586924826e-06 NA NA NA hsa-miR-20a-5p CSF1 0.854326758727003 5.04256669663285e-07 -0.5853277886696 0.00153229238014141 miRNATAP -0.281846084837308 5.48117776284526e-08 NA NA NA hsa-miR-93-5p CSF1 1.39518349670953 2.84167728726712e-25 -0.5853277886696 0.00153229238014141 miRNATAP -0.220842943300629 0.000492146206942775 NA NA NA hsa-miR-107 CSF1R 0.241662461253461 0.0170788899505389 -1.22973996011932 1.03080978200997e-10 miRanda -0.446705177499975 1.81867568630625e-06 NA NA NA hsa-miR-30d-5p DDIT4 0.718283816348023 2.47422074061352e-08 0.228443977506799 0.236844454905723 MirTarget;miRNATAP -0.20537756673444 0.00438989200660324 NA NA NA hsa-miR-32-5p DDIT4 0.0806126674664016 0.548984992497432 0.228443977506799 0.236844454905723 miRNATAP -0.199880895095326 0.00445876889151134 NA NA NA hsa-miR-92a-3p DDIT4 0.212391012518195 0.134292268089997 0.228443977506799 0.236844454905723 miRNATAP -0.182713424395714 0.00604393810305066 NA NA NA hsa-miR-30c-2-3p EFNA1 -1.39811819128587 3.66376924527665e-15 0.646825904608858 3.64944927546855e-05 MirTarget -0.234024324762841 1.39767068736484e-08 NA NA NA hsa-miR-326 EFNA1 -1.881935132554 1.68087316863775e-21 0.646825904608858 3.64944927546855e-05 miRanda -0.140434021609749 0.000161056439179517 NA NA NA hsa-miR-486-5p EFNA1 -1.77766890600022 2.9071717929922e-14 0.646825904608858 3.64944927546855e-05 miRanda -0.149419446907336 2.19123125151434e-06 NA NA NA hsa-miR-145-5p EFNA3 -1.4795885711477 6.47308361543414e-16 1.74857523726189 1.89670377349898e-17 miRNATAP -0.27893442549449 2.94867753331604e-07 NA NA NA hsa-miR-27b-3p EFNA3 -0.823491817819754 9.20935302746823e-14 1.74857523726189 1.89670377349898e-17 miRNATAP -0.583190806894562 1.14503674353614e-10 NA NA NA hsa-miR-30a-5p EFNA3 -0.626257932887855 0.000108198196973765 1.74857523726189 1.89670377349898e-17 MirTarget;mirMAP;miRNATAP -0.324845227158913 3.1494534591563e-07 NA NA NA hsa-miR-30c-5p EFNA3 -0.432111642767632 0.000159764714931007 1.74857523726189 1.89670377349898e-17 MirTarget;mirMAP;miRNATAP -0.272889750837349 0.00277347446519368 NA NA NA hsa-miR-30e-5p EFNA3 -0.627815042856657 3.02205690184908e-10 1.74857523726189 1.89670377349898e-17 MirTarget;mirMAP -0.485030662588513 2.3026107671749e-06 NA NA NA hsa-miR-326 EFNA3 -1.881935132554 1.68087316863775e-21 1.74857523726189 1.89670377349898e-17 MirTarget;miRanda;miRNATAP -0.176452904716396 0.000484752208263276 NA NA NA hsa-miR-342-5p EFNA3 -0.780720763816797 5.21703298730986e-07 1.74857523726189 1.89670377349898e-17 miRNATAP -0.194650872773775 0.00339775056957733 NA NA NA hsa-miR-3607-3p EFNA3 -2.15518588554493 1.03390728226728e-23 1.74857523726189 1.89670377349898e-17 mirMAP;miRNATAP -0.201114949852214 1.03301411116055e-05 NA NA NA hsa-miR-486-5p EFNA3 -1.77766890600022 2.9071717929922e-14 1.74857523726189 1.89670377349898e-17 miRanda -0.222051028105411 2.00549553126369e-07 NA NA NA hsa-miR-1976 EFNA4 -0.431472440543166 0.00324575010550946 1.47899606476918 8.0933783233146e-26 MirTarget -0.18479067692524 0.000194314415527299 NA NA NA hsa-miR-26b-5p EFNA4 -1.10804856399744 4.14176110314282e-22 1.47899606476918 8.0933783233146e-26 miRNAWalker2_validate -0.351313032976239 4.29536860806307e-09 NA NA NA hsa-miR-101-3p EFNA5 -1.48176407980989 3.98950707385704e-31 -1.35514027226431 0.000692469246595569 miRNATAP -0.628698296210383 9.0433663712587e-06 NA NA NA hsa-miR-1269a EFNA5 3.57543539205634 4.25369619949175e-08 -1.35514027226431 0.000692469246595569 mirMAP -0.118790518480963 0.000117551955405591 NA NA NA hsa-miR-19a-3p EFNA5 1.01712596153672 2.57753201450625e-07 -1.35514027226431 0.000692469246595569 miRNATAP -0.279630002756674 0.0042407624166513 NA NA NA hsa-miR-19b-3p EFNA5 0.601279551368824 0.00016685097085251 -1.35514027226431 0.000692469246595569 miRNATAP -0.40467492977246 0.00088980599521387 NA NA NA hsa-miR-204-5p EFNA5 -0.537962150012103 0.0330895493358849 -1.35514027226431 0.000692469246595569 miRNATAP -0.393603225156653 2.80940732573529e-07 NA NA NA hsa-miR-28-5p EFNA5 -0.430964830599964 2.67367257252925e-06 -1.35514027226431 0.000692469246595569 PITA;miRNATAP -0.961268745350365 5.76123363646996e-06 NA NA NA hsa-miR-32-3p EFNA5 0.219109440121582 0.207217051619543 -1.35514027226431 0.000692469246595569 mirMAP -0.398640476166263 0.000613468003303945 NA NA NA hsa-miR-362-3p EFNA5 0.814007335895913 2.00108618836568e-09 -1.35514027226431 0.000692469246595569 miRanda -0.406503595240295 0.00419808680650827 NA NA NA hsa-miR-378a-3p EFNA5 -1.1920055906972 3.23538871733634e-10 -1.35514027226431 0.000692469246595569 miRNATAP -0.371127828088677 0.000229858039216613 NA NA NA hsa-miR-378c EFNA5 -1.54028191929038 2.70131554214866e-15 -1.35514027226431 0.000692469246595569 miRNATAP -0.259859617515804 0.00742413150669568 NA NA NA hsa-miR-92a-3p EFNA5 0.212391012518195 0.134292268089997 -1.35514027226431 0.000692469246595569 miRNAWalker2_validate -0.490918489487089 0.000381023755947929 NA NA NA hsa-miR-146a-5p EGFR -0.742958238440575 0.000765983177482633 -1.01672920195188 6.69873186671412e-07 miRNAWalker2_validate -0.224421920698198 6.22524592924969e-07 24895573;25242818;22161865;25596948;25417703;23555954;24839931;21632853 Furthermore western blot showed that miR-146a mimic downregulated EGFR ERK1/2 and stat5 signalings; These effects were less potent compared to that of a siRNA targeting EGFR a known target gene of miR-146a; Moreover miR-146a mimic could enhance the cell growth inhibition and apoptosis induction impact of various EGFR targeting agents;Interestingly re-expression of miR-146a inhibited the invasive capacity of Colo357 and Panc-1 PC cells with concomitant down-regulation of EGFR and IRAK-1; Most importantly we found that the treatment of PC cells with "natural agents" 33'-diinodolylmethane DIM or isoflavone led to an increase in the expression of miR-146a and consequently down-regulated the expression of EGFR MTA-2 IRAK-1 and NF-κB resulting in the inhibition of invasion of Colo357 and Panc-1 cells;MiR 146a suppresses tumor growth and progression by targeting EGFR pathway and in a p ERK dependent manner in castration resistant prostate cancer; Mechanistic studies revealed that miR-146a repressed the expression of EGFR through binding to its 3'-untranslated region; Our findings suggest that ubiquitous loss of miR-146a is a critical mechanism for overexpression of EGFR in CRPC which is crucial to better understanding the pathogenesis of CRPC;Furthermore the expressions of bax and cleaved-caspase-3 mainly were increased in control and overexpression miR-146a groups however the expression of EGFR was inverse; All the results demonstrated that quercetin exhibited excellent effect on inhibiting cell proliferation in human breast cancer cells which was performed by up-regulating miR-146a expression then via inducing apoptosis through caspase-3 activation and mitochondrial-dependent pathways and inhibiting invasion through down-regulating the expression of EGFR;Here we report a previously unrecognized posttranscriptional mechanism by which BRCA1 regulates EGFR expression through the induction of miR-146a; We show that BRCA1 binds to MIR146A promoter and activates transcription which in turn attenuates EGFR expression;In functional experiments miR-146a suppressed cell growth induced cellular apoptosis and inhibited EGFR downstream signaling in five NSCLC cell lines H358 H1650 H1975 HCC827 and H292 miR-146a also inhibited the migratory capacity of these NSCLC cells; On the other hand miR-146a enhanced the inhibition of cell proliferation by drugs targeting EGFR including both TKIs gefitinib erlotinib and afatinib and a monoclonal antibody cetuximab; Our results suggest that these effects of miR-146a are due to its targeting of EGFR and NF-κB signaling;Deregulation of miR 146a expression in a mouse model of pancreatic cancer affecting EGFR signaling; Treatment of PC cells with CDF a novel synthetic compound led to re-expression of miR-146a resulting in the down-regulation of EGFR expression; Further knock-down of miR-146a in AsPC-1 cells led to the up-regulation of EGFR expression and showed increased clonogenic growth; In addition knock-down of EGFR by EGFR siRNA transfection of parental AsPC-1 cells and AsPC-1 cells stably transfected with pre-miR-146a resulted in decreased invasive capacity which was further confirmed by reduced luciferase activity in cells transfected with pMIR-Luc reporter vector containing miR-146a binding site; Collectively these results suggest that the loss of expression of miR-146a is a fundamental mechanism for over-expression of EGFR signaling and that re-expression of miR-146a by CDF treatment could be useful in designing personalized strategy for the treatment of human PC;The regulation of EGFR and IRAK1 by miR-146a was examined with miR-146a-transfected gastric cancer cells; Ectopic expression of miR-146a inhibited migration and invasion and downregulated EGFR and IRAK1 expression in gastric cancer cells; MiR-146a targeting of EGFR and IRAK1 is an independent prognostic factor in gastric cancer cases ;;progression;;;;; liver cancer;pancreatic cancer;prostate cancer;breast cancer;breast cancer;lung squamous cell cancer;pancreatic cancer;gastric cancer hsa-miR-146b-5p EGFR 0.419719546795449 0.0457375823220155 -1.01672920195188 6.69873186671412e-07 miRNATAP -0.221138858293073 3.44879306513365e-06 NA NA NA hsa-miR-148b-5p EGFR 0.295777213389605 0.0255749859535438 -1.01672920195188 6.69873186671412e-07 mirMAP -0.233539600600063 0.00227279523782391 NA NA NA hsa-miR-17-5p EGFR 0.698861147045461 2.43854106455398e-05 -1.01672920195188 6.69873186671412e-07 TargetScan -0.161590563481239 0.00764134099999989 NA NA NA hsa-miR-188-5p EGFR 1.11646899351496 9.56686958408598e-11 -1.01672920195188 6.69873186671412e-07 mirMAP -0.300235014857475 1.22424681244032e-07 NA NA NA hsa-miR-21-5p EGFR 1.51084295924322 1.54551622173259e-34 -1.01672920195188 6.69873186671412e-07 miRNAWalker2_validate;miRTarBase -0.440371363258275 3.21604130567409e-09 24198203;20113523;24012640;24331411;26563758;19597153;26026961;20048743 In radically resected NSCLC patients the expression levels of miR-21 10b in patients with EGFR mutation were much higher than those without mutation;Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;Further the expression of miR-21 is regulated by EGFR via the activation of β-catenin and AP-1; These data indicate that a feedback loop exists between miR-21 and EGFR; These results clarify a novel association between miR-21 and EGFR in the regulation of cancer cell progression;MiR 21 overexpression is associated with acquired resistance of EGFR TKI in non small cell lung cancer;Higher expression levels of miR-21 AmiR-27a and miR-218 detected in this study suggest potential roles of these miRNAs in primary resistance to EGFR-TKI in advanced NSCLC patients with EGFR exon 19 deletion mutations;MiR 21 is an EGFR regulated anti apoptotic factor in lung cancer in never smokers; The changes in expression of some of these miRNAs including miR-21 were more remarkable in cases with EGFR mutations than in those without these mutations; In the never-smoker-derived lung adenocarcinoma cell line H3255 with mutant EGFR and high levels of p-EGFR and miR-21 antisense inhibition of miR-21 enhanced AG1478-induced apoptosis; In a never-smoker-derived adenocarcinoma cell line H441 with wild-type EGFR the antisense miR-21 not only showed the additive effect with AG1478 but also induced apoptosis by itself; These results suggest that aberrantly increased expression of miR-21 which is enhanced further by the activated EGFR signaling pathway plays a significant role in lung carcinogenesis in never-smokers as well as in smokers and is a potential therapeutic target in both EGFR-mutant and wild-type cases;Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR mutated lung cancer via nickel induced microRNA 21 expression;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status ;;progression;drug resistance;staging;drug resistance;tumorigenesis;; lung squamous cell cancer;glioblastoma;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;lung cancer;lung cancer;glioblastoma hsa-miR-29a-5p EGFR -0.113555908626946 0.349622266470788 -1.01672920195188 6.69873186671412e-07 mirMAP -0.458889085026057 2.82252978142708e-08 27477273 Feedback Loop Regulation of SCAP/SREBP 1 by miR 29 Modulates EGFR Signaling Driven Glioblastoma Growth glioblastoma hsa-miR-32-3p EGFR 0.219109440121582 0.207217051619543 -1.01672920195188 6.69873186671412e-07 mirMAP -0.238124126347676 6.9947851263588e-05 NA NA NA hsa-miR-362-5p EGFR 0.717921690799312 1.68392734504524e-05 -1.01672920195188 6.69873186671412e-07 mirMAP -0.247853042376492 3.33470814837779e-05 NA NA NA hsa-miR-374b-3p EGFR -0.137420230218985 0.305318192448799 -1.01672920195188 6.69873186671412e-07 mirMAP -0.202869152527087 0.00750806275200147 NA NA NA hsa-miR-455-5p EGFR -0.270546034117157 0.0581320703577731 -1.01672920195188 6.69873186671412e-07 miRanda -0.226922724788645 0.00135818927133814 NA NA NA hsa-miR-501-5p EGFR 1.15231083477882 2.98906150004088e-10 -1.01672920195188 6.69873186671412e-07 mirMAP -0.228828416518186 2.10663471825952e-05 NA NA NA hsa-miR-590-5p EGFR -0.103355559980404 0.310027295317092 -1.01672920195188 6.69873186671412e-07 miRanda;mirMAP -0.282971808483034 0.00478577902454241 NA NA NA hsa-miR-769-5p EGFR 0.219661452072771 0.0333425465750576 -1.01672920195188 6.69873186671412e-07 mirMAP -0.501593765874938 2.76004531715872e-07 NA NA NA hsa-miR-877-5p EGFR 1.35669625926821 1.43008125755656e-11 -1.01672920195188 6.69873186671412e-07 mirMAP -0.247141034396675 1.1588258665615e-06 NA NA NA hsa-miR-330-5p EIF4B 0.442231058604099 0.0053308560843808 -0.213371036122907 0.00927846005206924 miRNATAP -0.121583930567148 1.00720259566484e-06 NA NA NA hsa-miR-339-5p EIF4B 0.280589919878422 0.0355660282647215 -0.213371036122907 0.00927846005206924 miRanda -0.131202132226587 1.04386561466155e-05 NA NA NA hsa-miR-342-3p EIF4B -0.319677314984262 0.0449813878317147 -0.213371036122907 0.00927846005206924 miRanda -0.127381134711691 2.69280086896855e-07 NA NA NA hsa-miR-362-3p EIF4B 0.814007335895913 2.00108618836568e-09 -0.213371036122907 0.00927846005206924 miRanda -0.120133065959791 2.94856447934838e-05 NA NA NA hsa-miR-484 EIF4B 0.0857240033709656 0.453982056282229 -0.213371036122907 0.00927846005206924 miRNAWalker2_validate -0.156463221852403 7.2363961844545e-06 NA NA NA hsa-miR-497-5p EIF4B -1.41371173021948 7.09278946477743e-17 -0.213371036122907 0.00927846005206924 miRNATAP -0.103808529523177 3.87646612451936e-06 NA NA NA hsa-miR-107 EIF4E 0.241662461253461 0.0170788899505389 -0.423618150345152 8.76394952666589e-09 miRanda -0.145690630779375 5.10144854982989e-05 NA NA NA hsa-miR-25-3p EIF4E 0.633062563556379 3.68686354834328e-10 -0.423618150345152 8.76394952666589e-09 mirMAP -0.120897171896447 0.000649613904023936 NA NA NA hsa-miR-500a-3p EIF4E 1.18913245435489 1.59516890341346e-16 -0.423618150345152 8.76394952666589e-09 MirTarget -0.100990657027664 2.96319587641026e-05 NA NA NA hsa-miR-660-5p EIF4E 0.989755850734071 5.69930767464937e-14 -0.423618150345152 8.76394952666589e-09 mirMAP -0.155553210664168 3.85299588654816e-09 NA NA NA hsa-miR-140-5p EIF4E2 -0.220106443070144 0.0140713197101242 -0.0307165992319307 0.660560144350934 miRanda -0.138995836241305 0.000258086393762208 NA NA NA hsa-miR-29a-3p EIF4E2 -0.856762511178136 4.65330336378459e-12 -0.0307165992319307 0.660560144350934 MirTarget -0.113597041826362 1.87271499311999e-05 NA NA NA hsa-miR-125b-5p EIF4EBP1 -1.35767721102906 1.16564617395164e-18 -0.0296151122465442 0.838078981735608 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.116832363024321 0.00778246256108478 17891175;26646586 Using a bioinformatics approach we have identified additional potential mRNA targets of one of the miRNAs miR-125b that are upregulated in prostate cancer and confirmed increased expression of one of these targets EIF4EBP1 in prostate cancer tissues;Increased expression of miR-125a and miR-125b inhibited invasion and migration of SKOV3 and OVCAR-429 ovarian cancer cells and was associated with a decrease in EIF4EBP1 expression ; prostate cancer;ovarian cancer hsa-miR-34a-5p EPHA2 1.03694231790865 5.74864019540594e-14 -2.12469060332663 2.01316632998596e-28 miRNAWalker2_validate -0.219033843498792 0.00193427032704837 NA NA NA hsa-miR-361-5p F2R 0.233202959858696 0.00961572812255327 -0.675603600685401 0.000189133295700278 miRanda -0.410778924266647 3.41580887465187e-05 NA NA NA hsa-miR-589-3p F2R 1.16846704028095 5.88525574424201e-11 -0.675603600685401 0.000189133295700278 MirTarget -0.165119595940011 0.000869583163579348 NA NA NA hsa-miR-590-5p F2R -0.103355559980404 0.310027295317092 -0.675603600685401 0.000189133295700278 miRanda -0.385360300348432 1.07401755923676e-05 NA NA NA hsa-miR-25-3p FASLG 0.633062563556379 3.68686354834328e-10 -0.789267794109529 0.000910923617828174 miRNATAP -0.409147266425705 0.000319496225971281 NA NA NA hsa-miR-16-1-3p FGF1 0.392360028038559 0.00112481918108198 -0.678542882304208 0.00654328337233827 mirMAP -0.418480509125599 3.32517568843891e-05 NA NA NA hsa-miR-186-5p FGF1 -0.060103391373687 0.535288717081205 -0.678542882304208 0.00654328337233827 mirMAP -0.41367102247344 0.00114927327556726 NA NA NA hsa-miR-18a-5p FGF1 0.918028187958706 1.90229510425322e-05 -0.678542882304208 0.00654328337233827 MirTarget -0.257554404550762 3.95122313648269e-06 NA NA NA hsa-miR-361-5p FGF1 0.233202959858696 0.00961572812255327 -0.678542882304208 0.00654328337233827 miRanda -0.510174670094289 0.000185533193035221 NA NA NA hsa-miR-550a-5p FGF1 0.156224023171823 0.282505553619229 -0.678542882304208 0.00654328337233827 MirTarget -0.374323243103714 7.86437510357121e-06 NA NA NA hsa-miR-576-5p FGF1 -0.377452008285847 0.00470735657343984 -0.678542882304208 0.00654328337233827 PITA;mirMAP -0.266396057997619 0.00356602502679197 NA NA NA hsa-miR-590-5p FGF1 -0.103355559980404 0.310027295317092 -0.678542882304208 0.00654328337233827 MirTarget;miRanda;miRNATAP -0.467961306585168 0.000104595555173684 NA NA NA hsa-miR-193b-3p FGF11 -0.166697207281748 0.272022543069482 0.213353925569267 0.274831508108713 miRNAWalker2_validate -0.200531716988523 0.00140906192264391 NA NA NA hsa-miR-28-5p FGF11 -0.430964830599964 2.67367257252925e-06 0.213353925569267 0.274831508108713 miRanda -0.376056857807372 0.00027660131559535 NA NA NA hsa-miR-548b-3p FGF11 -0.0203576084717134 0.930819503166956 0.213353925569267 0.274831508108713 mirMAP -0.150043977025028 0.00025412409602433 NA NA NA hsa-miR-17-5p FGF12 0.698861147045461 2.43854106455398e-05 1.12701565396445 0.000125070474588253 TargetScan -0.353312293407124 4.00239309386818e-05 NA NA NA hsa-miR-200b-3p FGF12 -1.29335788099229 0.000268623234305059 1.12701565396445 0.000125070474588253 TargetScan -0.167486025000735 2.91497216782912e-05 NA NA NA hsa-miR-429 FGF12 -1.40262535827285 6.93982219171912e-05 1.12701565396445 0.000125070474588253 miRNATAP -0.176507119934699 1.51329896482206e-05 NA NA NA hsa-miR-455-5p FGF12 -0.270546034117157 0.0581320703577731 1.12701565396445 0.000125070474588253 miRanda -0.2955118739476 0.00352688948327299 NA NA NA hsa-miR-483-3p FGF12 -2.99328900859484 2.67538108509603e-07 1.12701565396445 0.000125070474588253 PITA -0.119968404362397 8.50806205124405e-07 NA NA NA hsa-miR-889-3p FGF12 -0.704721025765331 0.0139978010941786 1.12701565396445 0.000125070474588253 MirTarget -0.131935954446475 0.00860562658075859 NA NA NA hsa-miR-455-5p FGF13 -0.270546034117157 0.0581320703577731 1.04387202836938 0.000243713997792116 PITA;miRanda -0.315950161217624 0.00124978729248725 NA NA NA hsa-miR-193a-5p FGF14 -0.472805720224262 1.24962968905481e-05 -0.650638914328184 0.0386566233872504 TargetScan -0.396389198365541 0.00514712574405294 NA NA NA hsa-miR-362-3p FGF14 0.814007335895913 2.00108618836568e-09 -0.650638914328184 0.0386566233872504 miRanda -0.296503371024151 0.00779105168383237 NA NA NA hsa-miR-421 FGF14 0.941856334694081 6.12167297054077e-08 -0.650638914328184 0.0386566233872504 miRanda -0.459990027128502 9.7647751856669e-08 NA NA NA hsa-miR-15b-3p FGF2 0.238105490431193 0.0997721500357416 -1.08625533231299 0.000324809942833161 mirMAP -0.499213807743807 9.41955107470528e-07 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-15b-5p FGF2 0.230566941413564 0.0824765001857479 -1.08625533231299 0.000324809942833161 MirTarget;miRNATAP -0.306809751282981 0.00622064491645458 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-16-2-3p FGF2 -0.0336420826463706 0.805158952566973 -1.08625533231299 0.000324809942833161 mirMAP -0.422467965590387 0.000103679884556575 NA NA NA hsa-miR-181a-5p FGF2 0.250113435902954 0.0551933249319293 -1.08625533231299 0.000324809942833161 mirMAP -0.406045352280239 0.000358497143826792 NA NA NA hsa-miR-181b-5p FGF2 0.488256489401108 0.00105321129419701 -1.08625533231299 0.000324809942833161 mirMAP -0.523910277677257 9.1015168945214e-08 NA NA NA hsa-miR-19b-1-5p FGF2 -0.277804782598538 0.0783067794722916 -1.08625533231299 0.000324809942833161 mirMAP -0.529151998934904 1.16181517704286e-08 NA NA NA hsa-miR-200b-3p FGF2 -1.29335788099229 0.000268623234305059 -1.08625533231299 0.000324809942833161 mirMAP -0.168899172593471 4.11351056555817e-05 NA NA NA hsa-miR-200c-3p FGF2 -0.104675615392797 0.716957669484981 -1.08625533231299 0.000324809942833161 mirMAP -0.156084549253076 0.00233819769179705 NA NA NA hsa-miR-20a-3p FGF2 -0.318177901603857 0.0467885456739855 -1.08625533231299 0.000324809942833161 mirMAP -0.492065243098747 8.09310422865092e-08 NA NA NA hsa-miR-21-3p FGF2 -0.484219378716006 0.00299941098417659 -1.08625533231299 0.000324809942833161 mirMAP -0.240197113439825 0.00811114129230132 NA NA NA hsa-miR-21-5p FGF2 1.51084295924322 1.54551622173259e-34 -1.08625533231299 0.000324809942833161 mirMAP -0.443381765546842 5.91228766084995e-05 NA NA NA hsa-miR-217 FGF2 1.05797297540381 0.0401026692682422 -1.08625533231299 0.000324809942833161 miRanda -0.127490520581258 7.38698652450206e-06 NA NA NA hsa-miR-335-3p FGF2 -0.275199247483111 0.106629165248783 -1.08625533231299 0.000324809942833161 mirMAP -0.405107692314837 2.67658505476485e-06 NA NA NA hsa-miR-338-5p FGF2 -0.221898432884726 0.252390988727086 -1.08625533231299 0.000324809942833161 mirMAP -0.277477917380717 0.000298421894309671 NA NA NA hsa-miR-429 FGF2 -1.40262535827285 6.93982219171912e-05 -1.08625533231299 0.000324809942833161 mirMAP -0.192074793475977 4.45604159403906e-06 NA NA NA hsa-miR-452-5p FGF2 1.91544498799508 1.81995982853313e-14 -1.08625533231299 0.000324809942833161 mirMAP -0.254292038125162 7.84361502048493e-06 NA NA NA hsa-miR-589-3p FGF2 1.16846704028095 5.88525574424201e-11 -1.08625533231299 0.000324809942833161 mirMAP -0.346323302130209 2.68946343007257e-05 NA NA NA hsa-miR-92a-3p FGF2 0.212391012518195 0.134292268089997 -1.08625533231299 0.000324809942833161 miRNAWalker2_validate -0.635934402042256 6.72536311164143e-10 NA NA NA hsa-miR-93-3p FGF2 0.402821129107592 0.00131324842735588 -1.08625533231299 0.000324809942833161 mirMAP -0.437085459323848 0.000209985380897254 NA NA NA hsa-miR-107 FGF7 0.241662461253461 0.0170788899505389 -0.653895802717462 0.00602323864930533 PITA;miRanda;miRNATAP -0.317347847907002 0.0061558373813029 NA NA NA hsa-miR-15a-5p FGF7 0.349521211668244 0.000770735998804736 -0.653895802717462 0.00602323864930533 miRTarBase;miRNATAP -0.366019587762154 0.00111409761072969 NA NA NA hsa-miR-15b-5p FGF7 0.230566941413564 0.0824765001857479 -0.653895802717462 0.00602323864930533 miRNATAP -0.392337856862938 7.12966084973613e-06 NA NA NA hsa-miR-16-1-3p FGF7 0.392360028038559 0.00112481918108198 -0.653895802717462 0.00602323864930533 mirMAP -0.272657830128381 0.00482946390365832 NA NA NA hsa-miR-20a-5p FGF7 0.854326758727003 5.04256669663285e-07 -0.653895802717462 0.00602323864930533 miRNAWalker2_validate -0.341470101333498 3.32475785382172e-07 NA NA NA hsa-miR-361-5p FGF7 0.233202959858696 0.00961572812255327 -0.653895802717462 0.00602323864930533 PITA;miRanda;miRNATAP -0.415294917437999 0.00146403039846296 NA NA NA hsa-miR-421 FGF7 0.941856334694081 6.12167297054077e-08 -0.653895802717462 0.00602323864930533 miRanda -0.230227109673969 0.000489381552238336 NA NA NA hsa-miR-455-3p FGF7 -1.3998033821095 5.72010709806871e-17 -0.653895802717462 0.00602323864930533 PITA;miRNATAP -0.173570787405519 0.00961793201913047 NA NA NA hsa-miR-590-5p FGF7 -0.103355559980404 0.310027295317092 -0.653895802717462 0.00602323864930533 PITA;miRanda -0.57199782619933 5.74241805880331e-07 NA NA NA hsa-miR-616-5p FGF7 0.150569904638223 0.402836556304524 -0.653895802717462 0.00602323864930533 mirMAP -0.245435969787053 0.000149305105460564 NA NA NA hsa-miR-618 FGF7 0.13539895350352 0.517145913114103 -0.653895802717462 0.00602323864930533 mirMAP -0.227583154837617 7.48998921932753e-05 NA NA NA hsa-miR-106b-5p FGFR1 0.648901865047099 8.24870862047885e-09 -1.10351511026112 4.12675064572266e-07 mirMAP -0.284443073679231 0.0026248045594602 NA NA NA hsa-miR-15a-5p FGFR1 0.349521211668244 0.000770735998804736 -1.10351511026112 4.12675064572266e-07 miRNATAP -0.352840830794253 0.000677757595811867 21532615 Such downregulation of miR-15 and miR-16 in cancer-associated fibroblasts CAFs promoted tumor growth and progression through the reduced post-transcriptional repression of Fgf-2 and its receptor Fgfr1 which act on both stromal and tumor cells to enhance cancer cell survival proliferation and migration progression;poor survival prostate cancer hsa-miR-17-5p FGFR1 0.698861147045461 2.43854106455398e-05 -1.10351511026112 4.12675064572266e-07 mirMAP -0.237393252177173 0.000224064428361276 NA NA NA hsa-miR-20a-5p FGFR1 0.854326758727003 5.04256669663285e-07 -1.10351511026112 4.12675064572266e-07 mirMAP -0.287282538249605 3.68706352846777e-06 NA NA NA hsa-miR-361-5p FGFR1 0.233202959858696 0.00961572812255327 -1.10351511026112 4.12675064572266e-07 miRanda -0.33530909970249 0.00556370932111968 NA NA NA hsa-miR-455-5p FGFR1 -0.270546034117157 0.0581320703577731 -1.10351511026112 4.12675064572266e-07 miRanda -0.249494978181263 0.000949827332507152 NA NA NA hsa-miR-532-5p FGFR1 1.02729546510601 9.93318913966636e-16 -1.10351511026112 4.12675064572266e-07 PITA -0.504576508830531 2.82854123723416e-10 NA NA NA hsa-miR-93-5p FGFR1 1.39518349670953 2.84167728726712e-25 -1.10351511026112 4.12675064572266e-07 mirMAP -0.326905735165641 1.37221347364255e-05 NA NA NA hsa-miR-107 FGFR2 0.241662461253461 0.0170788899505389 -2.28582334954307 1.14765468936708e-05 PITA;miRanda -0.690301810710163 0.0068857261082219 NA NA NA hsa-miR-100-5p FGFR3 -0.783576782126614 0.000219958772262677 0.189024805500786 0.340204942325551 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.269596147351536 1.27205852375091e-09 25344675;26604796;26018508;23778527 MicroRNA 100 regulates pancreatic cancer cells growth and sensitivity to chemotherapy through targeting FGFR3; The predicted target of miR-100 fibroblast growth factor receptor 3 FGFR3 was downregulated by siRNA to examine its effect on pancreatic cancer cells; Luciferase essay showed FGFR3 was direct target of miR-100; FGFR3 was significantly downregulated by overexpressing miR-100 in pancreatic cancer cells and knocking down FGFR3 by siRNA exerted similar effect as miR-100; Our study demonstrated that miR-100 played an important role in pancreatic cancer development possibly through targeting FGFR3;Overexpression of miR 100 inhibits cell proliferation migration and chemosensitivity in human glioblastoma through FGFR3; Expression of fibroblast growth factor receptor 3 FGFR3 the bioinformatically predicted target of miR-100 was examined by Western blot in glioblastoma; FGFR3 was directly regulated by miR-100 in glioblastoma; Ectopically overexpressing FGFR3 was able to ameliorate the anticancer effects of upregulation of miR-100 on glioblastoma growth migration and chemosensitivity; Overexpressing miR-100 had anticancer effects on glioblastoma likely through regulation of FGFR3;Overexpression of miR 100 inhibits growth of osteosarcoma through FGFR3; Here we reported significantly higher levels of fibroblast growth factor receptor 3 FGFR3 and significantly lower levels of miR-100 in the OS specimen compared to those in the paired normal bone tissues; Bioinformatics analysis and luciferase reporter assay suggest that miR-100 binds to the 3'UTR of FGFR3 mRNA to prevent its translation; Taken together our data demonstrate that miR-100 may inhibit the growth of OS through FGFR3;Hypoxia regulates FGFR3 expression via HIF 1α and miR 100 and contributes to cell survival in non muscle invasive bladder cancer; We have previously investigated the role of microRNAs in bladder cancer and have shown that FGFR3 is a target of miR-100; In this study we investigated the effects of hypoxia on miR-100 and FGFR3 expression and the link between miR-100 and FGFR3 in hypoxia; Bladder cancer cell lines were exposed to normoxic or hypoxic conditions and examined for the expression of FGFR3 by quantitative PCR qPCR and western blotting and miR-100 by qPCR; The effect of FGFR3 and miR-100 on cell viability in two-dimensional 2-D and three-dimensional 3-D was examined by transfecting siRNA or mimic-100 respectively; Increased FGFR3 was also in part dependent on miR-100 levels which decreased in hypoxia; Hypoxia in part via suppression of miR-100 induces FGFR3 expression in bladder cancer both of which have an important role in maintaining cell viability under conditions of stress ;;;poor survival pancreatic cancer;glioblastoma;sarcoma;bladder cancer hsa-miR-99a-5p FGFR3 -1.51481656384839 3.93230057259529e-14 0.189024805500786 0.340204942325551 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.302096873169716 3.68370160731018e-11 23409016;24456664;23298836 Photofrin based photodynamic therapy and miR 99a transfection inhibited FGFR3 and PI3K/Akt signaling mechanisms to control growth of human glioblastoma In vitro and in vivo; Further photofrin based PDT followed by miR-99a transfection dramatically increased miR-99a expression and also increased apoptosis in glioblastoma cell cultures and drastically reduced tumor growth in athymic nude mice due to down regulation of fibroblast growth factor receptor 3 FGFR3 and PI3K/Akt signaling mechanisms leading to inhibition of cell proliferation and induction of molecular mechanisms of apoptosis;miR 99a promotes proliferation targeting FGFR3 in human epithelial ovarian cancer cells; Importantly fibroblast growth factor receptor 3 FGFR3 predicted to be one target gene of miR-99a using computational algorithms was higher in expression in EOC cells; Subsequently FGFR3 was proved to be direct target of miR-99a by dual luciferase assay; Furthermore overexpression of miR-99a dramatically suppressed expression level of FGFR3 at both mRNA and protein levels proving FGFR3 to be inversely correlated with miR-99a; Finally overexpression of miR-99a could significantly inhibit EOC cell proliferation in vitro by decreasing the expression of FGFR3 which also reduced the EOC cell growth after siRNA knockdown; Conclusively miR-99a expression was remarkably downregulated in serums tissues and cell and suppresses EOC cell proliferation by targeting FGFR3 suggesting miR-99a as a prospective prognosis marker and potential tumor suppressor for EOC therapeutics;The tumorigenic FGFR3 TACC3 gene fusion escapes miR 99a regulation in glioblastoma; The fusion caused by tandem duplication on 4p16.3 led to the loss of the 3'-UTR of FGFR3 blocking gene regulation of miR-99a and enhancing expression of the fusion gene ;worse prognosis; glioblastoma;ovarian cancer;glioblastoma hsa-miR-326 FGFR4 -1.881935132554 1.68087316863775e-21 0.491652034961419 0.019783553566567 miRanda;miRNATAP -0.275615867301029 2.26792389115162e-08 NA NA NA hsa-miR-491-5p FGFR4 -0.369122930844418 0.0154047757048929 0.491652034961419 0.019783553566567 miRanda -0.246783584450743 0.000288636596057226 NA NA NA hsa-miR-106b-5p FLT1 0.648901865047099 8.24870862047885e-09 0.348253937407654 0.00775687550206773 MirTarget;miRNATAP -0.271203439813761 1.04770226280036e-06 NA NA NA hsa-miR-17-5p FLT1 0.698861147045461 2.43854106455398e-05 0.348253937407654 0.00775687550206773 MirTarget;TargetScan;miRNATAP -0.155467412569947 4.41226191642039e-05 NA NA NA hsa-miR-19b-3p FLT1 0.601279551368824 0.00016685097085251 0.348253937407654 0.00775687550206773 MirTarget -0.106879008818879 0.00722041764726757 NA NA NA hsa-miR-20a-5p FLT1 0.854326758727003 5.04256669663285e-07 0.348253937407654 0.00775687550206773 MirTarget;miRNATAP -0.123502068793973 0.000848936793187574 NA NA NA hsa-miR-29a-5p FLT1 -0.113555908626946 0.349622266470788 0.348253937407654 0.00775687550206773 MirTarget -0.210145329786256 6.66000381524097e-05 NA NA NA hsa-miR-3200-3p FLT1 1.40312896232918 1.8232142177606e-07 0.348253937407654 0.00775687550206773 MirTarget -0.104775339523284 1.64518826352629e-05 NA NA NA hsa-miR-339-5p FLT1 0.280589919878422 0.0355660282647215 0.348253937407654 0.00775687550206773 miRanda -0.208449503114887 1.2161998602826e-05 NA NA NA hsa-miR-361-5p FLT1 0.233202959858696 0.00961572812255327 0.348253937407654 0.00775687550206773 miRanda -0.221523162209773 0.00197692560008583 NA NA NA hsa-miR-744-3p FLT1 -0.866026958622846 2.43034431433797e-08 0.348253937407654 0.00775687550206773 MirTarget -0.187874043185053 2.92559559268988e-06 NA NA NA hsa-miR-93-5p FLT1 1.39518349670953 2.84167728726712e-25 0.348253937407654 0.00775687550206773 MirTarget;miRNATAP -0.205648379982088 3.80614007626687e-06 NA NA NA hsa-miR-374a-5p FLT4 0.016390363747302 0.869784217438621 -0.249744231852068 0.0849407092756091 mirMAP -0.256980012115421 0.000310181489933742 NA NA NA hsa-miR-374b-5p FLT4 -0.307538028546424 0.0030079617545972 -0.249744231852068 0.0849407092756091 mirMAP -0.195169979138197 0.00437956323796754 NA NA NA hsa-miR-125a-3p FN1 -0.841683158494395 3.51202687176603e-05 -0.485556836875999 0.000166548369488467 miRanda -0.100307990153966 0.00143868392098275 NA NA NA hsa-miR-29b-3p FOXO3 -0.348885528720517 0.0121441341291285 -0.397292803573268 0.000123881986293746 miRNATAP -0.147416048741358 4.87750412962158e-05 NA NA NA hsa-miR-125a-3p G6PC -0.841683158494395 3.51202687176603e-05 -1.88488951906391 2.56486640480655e-07 miRanda -0.615895313778941 2.67325582376957e-12 NA NA NA hsa-miR-200b-3p G6PC -1.29335788099229 0.000268623234305059 -1.88488951906391 2.56486640480655e-07 MirTarget;TargetScan -0.13399064419035 0.00804554754882444 NA NA NA hsa-miR-200c-3p G6PC -0.104675615392797 0.716957669484981 -1.88488951906391 2.56486640480655e-07 MirTarget -0.244076555440378 9.08865751877016e-05 NA NA NA hsa-miR-23a-3p G6PC -0.178875974460574 0.135978273540604 -1.88488951906391 2.56486640480655e-07 miRNAWalker2_validate;miRTarBase -1.06713731754967 5.2790644034528e-13 22318941 Stat3 mediated activation of microRNA 23a suppresses gluconeogenesis in hepatocellular carcinoma by down regulating glucose 6 phosphatase and peroxisome proliferator activated receptor gamma coactivator 1 alpha; The expression of microRNA miR-23a a candidate miR targeting PGC-1α and G6PC was up-regulated in the mouse liver tumors as well as in primary human HCC; We confirmed PGC-1α and G6PC as direct targets of miR-23a and their expressions negatively correlated with miR-23a expression in human HCCs; Based on these data we conclude that gluconeogenesis is severely compromised in HCC by IL6-Stat3-mediated activation of miR-23a which directly targets PGC-1α and G6PC leading to decreased glucose production liver cancer hsa-miR-330-5p G6PC 0.442231058604099 0.0053308560843808 -1.88488951906391 2.56486640480655e-07 miRanda -0.787004488696162 1.37187814828758e-12 NA NA NA hsa-miR-339-5p G6PC 0.280589919878422 0.0355660282647215 -1.88488951906391 2.56486640480655e-07 miRanda -0.455120631672191 0.000796548785496086 NA NA NA hsa-miR-122-5p G6PC3 -1.23907441373955 4.42181244888642e-06 0.564713561170989 1.47276955755177e-08 miRNAWalker2_validate;miRTarBase;MirTarget -0.228822721874903 1.31371817626573e-45 NA NA NA hsa-miR-193a-5p G6PC3 -0.472805720224262 1.24962968905481e-05 0.564713561170989 1.47276955755177e-08 TargetScan -0.282548327670779 2.42155827588977e-10 NA NA NA hsa-let-7e-5p GHR 0.0350298755544731 0.811068614727804 -3.0731350188505 2.37156410493146e-28 miRNATAP -0.377255073576678 0.000145575955630923 NA NA NA hsa-miR-15a-5p GHR 0.349521211668244 0.000770735998804736 -3.0731350188505 2.37156410493146e-28 MirTarget;miRNATAP -0.550186371213254 8.39585599580478e-05 NA NA NA hsa-miR-29a-5p GHR -0.113555908626946 0.349622266470788 -3.0731350188505 2.37156410493146e-28 mirMAP;miRNATAP -0.363891052104852 0.00254054547194364 NA NA NA hsa-miR-361-5p GHR 0.233202959858696 0.00961572812255327 -3.0731350188505 2.37156410493146e-28 miRanda -0.686703580148507 2.33974492023957e-05 NA NA NA hsa-miR-421 GHR 0.941856334694081 6.12167297054077e-08 -3.0731350188505 2.37156410493146e-28 miRanda -0.75730282604987 6.46899530113514e-22 NA NA NA hsa-miR-455-5p GNB1 -0.270546034117157 0.0581320703577731 0.0958557110185971 0.13435411812339 miRanda -0.105145596215111 1.02847761734852e-06 NA NA NA hsa-miR-23b-3p GNB4 -0.530561587109023 5.43807206823652e-07 -0.928192910776987 2.12212917190083e-10 mirMAP -0.212892947304196 0.00183143660701724 NA NA NA hsa-miR-33a-3p GNB4 -0.683025859637737 1.01106097756419e-05 -0.928192910776987 2.12212917190083e-10 MirTarget -0.176715402998075 0.000146769007869337 NA NA NA hsa-miR-361-5p GNB4 0.233202959858696 0.00961572812255327 -0.928192910776987 2.12212917190083e-10 miRanda;miRNATAP -0.317825495404 9.33942049722369e-05 NA NA NA hsa-miR-616-5p GNB4 0.150569904638223 0.402836556304524 -0.928192910776987 2.12212917190083e-10 MirTarget -0.222542753601043 2.78176277489519e-08 NA NA NA hsa-miR-93-5p GNB4 1.39518349670953 2.84167728726712e-25 -0.928192910776987 2.12212917190083e-10 miRNAWalker2_validate -0.301938140081084 1.91212863286627e-09 NA NA NA hsa-miR-361-5p GNB5 0.233202959858696 0.00961572812255327 0.426876502902471 0.00235496717551496 miRanda -0.262212101044684 0.000646181117085778 NA NA NA hsa-miR-107 GNG12 0.241662461253461 0.0170788899505389 -0.749024820971969 9.12787292602269e-08 miRanda -0.352109955711477 2.43072591256035e-07 NA NA NA hsa-miR-338-3p GNG12 0.543135607704581 0.00461167747143826 -0.749024820971969 9.12787292602269e-08 miRanda -0.148937421383934 3.25125790279736e-05 NA NA NA hsa-miR-423-5p GNG12 0.698807775906485 1.02195196334612e-11 -0.749024820971969 9.12787292602269e-08 miRNAWalker2_validate -0.289354784524697 1.13499456269689e-05 NA NA NA hsa-miR-455-5p GNG12 -0.270546034117157 0.0581320703577731 -0.749024820971969 9.12787292602269e-08 miRanda -0.226520595073207 2.68617949312973e-06 NA NA NA hsa-miR-23b-3p GNG2 -0.530561587109023 5.43807206823652e-07 -0.850794425511732 6.29859635775606e-10 miRNATAP -0.213834398091893 0.000864226876006079 NA NA NA hsa-miR-455-5p GNG2 -0.270546034117157 0.0581320703577731 -0.850794425511732 6.29859635775606e-10 miRanda -0.229407106619483 1.4480956300012e-06 NA NA NA hsa-miR-618 GNG2 0.13539895350352 0.517145913114103 -0.850794425511732 6.29859635775606e-10 PITA;miRNATAP -0.159357772778274 2.17753784300249e-06 NA NA NA hsa-miR-193b-3p GNG4 -0.166697207281748 0.272022543069482 3.13065787191005 3.93511130749918e-08 mirMAP -0.727189516843406 9.21560908852491e-05 NA NA NA hsa-miR-199a-3p GNG4 -2.3279328685682 1.04468066198593e-17 3.13065787191005 3.93511130749918e-08 mirMAP -0.374794502377641 0.000158579044743277 NA NA NA hsa-miR-199b-3p GNG4 -2.33226605401203 9.51887435273314e-18 3.13065787191005 3.93511130749918e-08 mirMAP -0.375110385417779 0.000154799510229959 NA NA NA hsa-miR-23b-3p GNG4 -0.530561587109023 5.43807206823652e-07 3.13065787191005 3.93511130749918e-08 mirMAP -0.973422557249314 0.000234898535428262 NA NA NA hsa-miR-326 GNG4 -1.881935132554 1.68087316863775e-21 3.13065787191005 3.93511130749918e-08 mirMAP -0.493070991901794 0.000310359418335552 NA NA NA hsa-miR-542-3p GNG4 -1.31351015149842 3.64642423288576e-22 3.13065787191005 3.93511130749918e-08 mirMAP -0.569049863655344 0.00406660259271405 NA NA NA hsa-let-7a-5p GNG5 -0.328069937201898 0.000464344093744051 0.00889434951085377 0.914434057152244 MirTarget;TargetScan;miRNATAP -0.133605931047702 0.00189613241874912 NA NA NA hsa-let-7g-5p GNG5 -0.461294133285527 2.17210114729901e-05 0.00889434951085377 0.914434057152244 MirTarget;miRNATAP -0.124150824567995 0.000740157885149412 NA NA NA hsa-miR-100-5p GNG5 -0.783576782126614 0.000219958772262677 0.00889434951085377 0.914434057152244 miRNAWalker2_validate -0.111679571746108 1.44073402187179e-09 NA NA NA hsa-miR-141-3p GNG7 -0.354316381352484 0.256996894288799 -0.344922469297116 0.0809202837414247 MirTarget;TargetScan;miRNATAP -0.142045092870118 3.59031496857192e-06 NA NA NA hsa-miR-181a-2-3p GNG7 -0.232005557505191 0.236404981276858 -0.344922469297116 0.0809202837414247 mirMAP -0.167242874656 0.00065765335078702 NA NA NA hsa-miR-185-3p GNG7 -0.270845540360709 0.0853959272273836 -0.344922469297116 0.0809202837414247 mirMAP -0.235740423921735 0.00011242527300071 NA NA NA hsa-miR-18a-5p GNG7 0.918028187958706 1.90229510425322e-05 -0.344922469297116 0.0809202837414247 mirMAP -0.25413478130125 6.30826943853662e-09 NA NA NA hsa-miR-3127-5p GNG7 0.837385386388614 2.06679743975142e-07 -0.344922469297116 0.0809202837414247 mirMAP -0.280943833100497 1.58985906059257e-06 NA NA NA hsa-miR-330-5p GNG7 0.442231058604099 0.0053308560843808 -0.344922469297116 0.0809202837414247 mirMAP -0.266239080017072 9.77779269561468e-06 NA NA NA hsa-miR-338-5p GNG7 -0.221898432884726 0.252390988727086 -0.344922469297116 0.0809202837414247 PITA;miRNATAP -0.178234078899153 0.000350792048639832 NA NA NA hsa-miR-3615 GNG7 0.437674495807452 0.000852818785910106 -0.344922469297116 0.0809202837414247 mirMAP -0.326159279457622 7.52320602896003e-06 NA NA NA hsa-miR-3913-5p GNG7 0.247553671224336 0.062795011081188 -0.344922469297116 0.0809202837414247 mirMAP -0.413758257686458 8.23372292003807e-09 NA NA NA hsa-miR-590-3p GNG7 -0.473167498047808 1.76652934637227e-05 -0.344922469297116 0.0809202837414247 PITA;miRanda;mirMAP -0.337357170032764 0.000103056255264498 NA NA NA hsa-miR-616-5p GNG7 0.150569904638223 0.402836556304524 -0.344922469297116 0.0809202837414247 MirTarget -0.214703356428535 6.00489016444381e-05 NA NA NA hsa-miR-671-5p GNG7 0.843645373345787 3.92794196273313e-08 -0.344922469297116 0.0809202837414247 mirMAP -0.332238265928659 5.5235653910779e-08 NA NA NA hsa-miR-766-3p GNG7 0.199979324759329 0.257230732626744 -0.344922469297116 0.0809202837414247 MirTarget -0.242495661121196 7.90602635709586e-06 NA NA NA hsa-miR-940 GNG7 0.453020092551484 0.0177054921651997 -0.344922469297116 0.0809202837414247 MirTarget -0.292037147028017 1.09815456267002e-08 NA NA NA hsa-miR-219a-1-3p GNGT2 -0.065863458123071 0.645858701126434 -0.701598672056643 5.82578274783021e-05 MirTarget -0.260826527717683 1.27876547872272e-05 NA NA NA hsa-miR-29b-3p GSK3B -0.348885528720517 0.0121441341291285 0.11314223970595 0.0774493616232908 miRTarBase;miRNATAP -0.106852381323571 1.32787261855633e-06 NA NA NA hsa-miR-342-3p GSK3B -0.319677314984262 0.0449813878317147 0.11314223970595 0.0774493616232908 miRanda -0.106989468297279 2.44530655156687e-08 NA NA NA hsa-miR-122-5p GYS1 -1.23907441373955 4.42181244888642e-06 0.392873125602919 2.86401930220423e-05 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.189953182267256 1.56862390909274e-34 NA NA NA hsa-miR-194-3p GYS1 -0.772747277805343 2.9537465170191e-05 0.392873125602919 2.86401930220423e-05 MirTarget -0.191902681880689 8.6151082313614e-16 NA NA NA hsa-miR-26b-5p GYS1 -1.10804856399744 4.14176110314282e-22 0.392873125602919 2.86401930220423e-05 miRNAWalker2_validate -0.140430570182474 0.000235166950357895 NA NA NA hsa-miR-429 GYS2 -1.40262535827285 6.93982219171912e-05 -3.92334014237442 3.39924663084957e-16 miRanda -0.376733672309474 3.60566821932691e-08 NA NA NA hsa-miR-224-3p HGF 1.40770116795301 1.31426607144612e-08 -3.45738625921135 1.44942707549128e-24 mirMAP -0.24700467708681 0.000422016473692453 NA NA NA hsa-miR-30d-5p HGF 0.718283816348023 2.47422074061352e-08 -3.45738625921135 1.44942707549128e-24 mirMAP -0.636747615843547 1.6663430655982e-06 NA NA NA hsa-miR-3682-3p HGF 0.805600143458555 8.7874424633131e-06 -3.45738625921135 1.44942707549128e-24 mirMAP -0.322825191529333 0.00118766446680551 NA NA NA hsa-miR-421 HGF 0.941856334694081 6.12167297054077e-08 -3.45738625921135 1.44942707549128e-24 miRanda -0.37483532054751 0.00015557960010186 NA NA NA hsa-miR-616-5p HGF 0.150569904638223 0.402836556304524 -3.45738625921135 1.44942707549128e-24 mirMAP -0.329885938670236 0.000709899554621757 NA NA NA hsa-let-7a-5p HRAS -0.328069937201898 0.000464344093744051 0.80680940319204 2.11618137601331e-10 miRNAWalker2_validate;miRTarBase -0.411742589318827 6.60009421951558e-10 20607356;23134218;18344688;20033209;19323605 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells;MicroRNA let 7a inhibits the proliferation and invasion of nonsmall cell lung cancer cell line 95D by regulating K Ras and HMGA2 gene expression; K-RAS and HMGA2 mRNA levels were significantly higher in the let-7a overexpressed group than those in the let-7a inhibited group p < 0.05; However the protein levels of K-RAS and HMGA2 were significantly lower in the let-7a overexpressed group than those in the let-7a inhibited group p < 0.05; We suppose that let-7a inhibits the proliferation and invasion of the cell line 95D by regulating the translation of K-RAS and HMGA2 mRNA not the transcription of the mRNA itself;Using an established orthotopic mouse lung cancer model we show that intranasal let-7 administration reduces tumor formation in vivo in the lungs of animals expressing a G12D activating mutation for the K-ras oncogene;k-Ras and c-Myc two key oncogenes in lung cancer have been found to be targeted by let-7 in vitro; The aim of the present study is to determine the effect of let-7a a member of let-7 family on the growth of lung cancer in vivo and to investigate whether let-7-induced suppression of k-Ras and c-Myc is involved in lung cancer; Overexpression of let-7a can inhibit the growth of lung cancer transplanted subcutaneously in nude mice by suppression of k-Ras and c-Myc;Because let-7 microRNA targets the K-ras oncogene we aimed to characterize let-7 expression and function in PDAC in vitro and in vivo; Restoring let-7 levels in cancer-derived cell lines strongly inhibits cell proliferation K-ras expression and mitogen-activated protein kinase activation but fails to impede tumor growth progression after intratumoral gene transfer or after implantation of Capan-1 cells stably overexpressing let-7 microRNA ;;;;progression glioblastoma;lung squamous cell cancer;lung cancer;lung cancer;pancreatic cancer hsa-let-7b-5p HRAS -0.959013717270803 5.95127281258643e-11 0.80680940319204 2.11618137601331e-10 miRTarBase -0.270772577779286 5.9733154777202e-11 20607356;20881268;18344688;20033209;19323605 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells;On the basis of these data we suggest that the downregulation of let-7b and let-7e targeting K-ras and the upregulation of miR-17* a CRC marker could be considered as candidate molecular markers of cetuximab resistance;Using an established orthotopic mouse lung cancer model we show that intranasal let-7 administration reduces tumor formation in vivo in the lungs of animals expressing a G12D activating mutation for the K-ras oncogene;k-Ras and c-Myc two key oncogenes in lung cancer have been found to be targeted by let-7 in vitro; The aim of the present study is to determine the effect of let-7a a member of let-7 family on the growth of lung cancer in vivo and to investigate whether let-7-induced suppression of k-Ras and c-Myc is involved in lung cancer;Because let-7 microRNA targets the K-ras oncogene we aimed to characterize let-7 expression and function in PDAC in vitro and in vivo; Restoring let-7 levels in cancer-derived cell lines strongly inhibits cell proliferation K-ras expression and mitogen-activated protein kinase activation but fails to impede tumor growth progression after intratumoral gene transfer or after implantation of Capan-1 cells stably overexpressing let-7 microRNA ;drug resistance;;;progression glioblastoma;colorectal cancer;lung cancer;lung cancer;pancreatic cancer hsa-miR-143-3p HRAS -0.584123869220553 0.000907217687997436 0.80680940319204 2.11618137601331e-10 miRNAWalker2_validate;miRTarBase -0.141161766298964 7.30370553951274e-05 21276449 The Evi1 microRNA 143 K Ras axis in colon cancer colon cancer hsa-miR-30c-5p HSP90AA1 -0.432111642767632 0.000159764714931007 0.358140551368157 9.85322237128818e-05 miRNAWalker2_validate -0.202157986427488 1.9883621574863e-07 NA NA NA hsa-miR-30e-5p HSP90AA1 -0.627815042856657 3.02205690184908e-10 0.358140551368157 9.85322237128818e-05 miRNAWalker2_validate -0.241505661403756 4.10597516818342e-08 NA NA NA hsa-let-7a-3p HSP90AB1 -0.568717557554068 1.00947202557819e-07 0.889713776257228 6.19114669909869e-19 miRNATAP -0.241701485603409 2.40883989322717e-07 NA NA NA hsa-let-7b-3p HSP90AB1 -1.21979213547786 5.84575581584603e-17 0.889713776257228 6.19114669909869e-19 miRNATAP -0.250258623939999 1.79427689854621e-14 NA NA NA hsa-miR-455-3p HSP90AB1 -1.3998033821095 5.72010709806871e-17 0.889713776257228 6.19114669909869e-19 miRNAWalker2_validate -0.1060610748927 0.000280397717598 NA NA NA hsa-miR-505-5p HSP90AB1 -0.768282569967001 1.14662846595407e-05 0.889713776257228 6.19114669909869e-19 MirTarget -0.175369328180102 5.66447338811247e-10 NA NA NA hsa-miR-139-5p IBSP -2.11299531796557 8.16407076415038e-28 1.92420097521516 4.21531465173415e-07 miRanda -0.741449456478811 2.42397138345038e-17 NA NA NA hsa-miR-361-5p IFNAR1 0.233202959858696 0.00961572812255327 -0.792062674909203 2.09239032499642e-23 miRanda -0.157033852704188 0.00059042956108057 NA NA NA hsa-miR-3913-5p IFNAR1 0.247553671224336 0.062795011081188 -0.792062674909203 2.09239032499642e-23 mirMAP -0.126885674032461 3.57212494027152e-05 NA NA NA hsa-miR-103a-2-5p IGF1 1.17085533927423 5.31418969120877e-09 -3.09093589123239 2.87389370715384e-21 mirMAP -0.263504392304743 0.00223342792049484 NA NA NA hsa-miR-15b-3p IGF1 0.238105490431193 0.0997721500357416 -3.09093589123239 2.87389370715384e-21 mirMAP -0.317736514699582 0.00624882495536213 NA NA NA hsa-miR-181a-5p IGF1 0.250113435902954 0.0551933249319293 -3.09093589123239 2.87389370715384e-21 mirMAP -0.504463915639783 8.50109868625984e-05 NA NA NA hsa-miR-181b-5p IGF1 0.488256489401108 0.00105321129419701 -3.09093589123239 2.87389370715384e-21 mirMAP -0.623738116833257 1.69561535563835e-08 NA NA NA hsa-miR-19a-3p IGF1 1.01712596153672 2.57753201450625e-07 -3.09093589123239 2.87389370715384e-21 MirTarget -0.400144846493838 1.38857829477185e-06 NA NA NA hsa-miR-19b-3p IGF1 0.601279551368824 0.00016685097085251 -3.09093589123239 2.87389370715384e-21 MirTarget -0.48361890440052 2.97870836726975e-06 NA NA NA hsa-miR-20a-3p IGF1 -0.318177901603857 0.0467885456739855 -3.09093589123239 2.87389370715384e-21 mirMAP -0.361896843485159 0.00054680673837891 NA NA NA hsa-miR-301a-3p IGF1 0.842910511640361 1.43446467417891e-06 -3.09093589123239 2.87389370715384e-21 MirTarget -0.644215183857533 3.25465618219484e-12 NA NA NA hsa-miR-3913-5p IGF1 0.247553671224336 0.062795011081188 -3.09093589123239 2.87389370715384e-21 mirMAP -0.347700367785319 0.00609623314715803 NA NA NA hsa-miR-421 IGF1 0.941856334694081 6.12167297054077e-08 -3.09093589123239 2.87389370715384e-21 PITA -0.472083397878338 5.5786740474017e-07 NA NA NA hsa-miR-454-3p IGF1 0.668749059573955 2.42670710515113e-06 -3.09093589123239 2.87389370715384e-21 MirTarget -0.659716525935208 1.08682616687143e-08 NA NA NA hsa-miR-940 IGF1 0.453020092551484 0.0177054921651997 -3.09093589123239 2.87389370715384e-21 MirTarget;PITA;miRNATAP -0.258190444399689 0.00419588966919949 NA NA NA hsa-miR-122-5p IGF1R -1.23907441373955 4.42181244888642e-06 -0.704819576268303 0.00353845530141102 miRNAWalker2_validate;miRTarBase;miRNATAP -0.36918930122591 5.43953757427132e-19 19726678;23056576 Expression of SRF or Igf1R partially reversed tumor suppressor function of miR-122;MiR 122 inhibits cell proliferation and tumorigenesis of breast cancer by targeting IGF1R; We then screened and identified a novel miR-122 target insulin-like growth factor 1 receptor IGF1R and it was further confirmed by luciferase assay; Similar to the restoring miR-122 expression IGF1R downregulation suppressed cell growth and cell-cycle progression whereas IGF1R overexpression rescued the suppressive effect of miR-122; In clinical specimens IGF1R was widely overexpressed and its mRNA levels were inversely correlated with miR-122 expression; Taken together our results demonstrate that miR-122 functions as a tumor suppressor and plays an important role in inhibiting the tumorigenesis through targeting IGF1R and regulating PI3K/Akt/mTOR/p70S6K pathway ;tumorigenesis;progression liver cancer;breast cancer hsa-miR-1269a IGF1R 3.57543539205634 4.25369619949175e-08 -0.704819576268303 0.00353845530141102 mirMAP -0.107248654580264 5.68720148437858e-09 NA NA NA hsa-miR-140-3p IGF1R 0.546012239205329 4.84578261098777e-11 -0.704819576268303 0.00353845530141102 mirMAP -0.466711989272837 0.000930505583515681 24039995 Integrated analysis identified IGF1R as a direct and functional target of miR-140; Knockdown of IGF1R inhibited cell proliferation and invasion resembling that of miR-140 overexpression while overexpression of IGF1R attenuated the function of miR-140 in NSCLC cells; Together our results highlight the significance of miR-140 and IGF1R in the development and progression of NSCLC progression lung squamous cell cancer hsa-miR-148a-5p IGF1R -0.767626696027761 1.08359477494797e-06 -0.704819576268303 0.00353845530141102 mirMAP -0.626014741533492 1.69198276385715e-18 NA NA NA hsa-miR-15a-5p IGF1R 0.349521211668244 0.000770735998804736 -0.704819576268303 0.00353845530141102 miRNATAP -0.46922259656765 3.61522699850867e-05 NA NA NA hsa-miR-16-1-3p IGF1R 0.392360028038559 0.00112481918108198 -0.704819576268303 0.00353845530141102 mirMAP -0.495470276550512 3.45124137757538e-07 NA NA NA hsa-miR-192-5p IGF1R -0.50188499512787 0.00344713341196035 -0.704819576268303 0.00353845530141102 miRNAWalker2_validate -0.5797159058919 1.53799651190084e-18 NA NA NA hsa-miR-193a-5p IGF1R -0.472805720224262 1.24962968905481e-05 -0.704819576268303 0.00353845530141102 mirMAP -0.335338989012095 0.00208565024368226 NA NA NA hsa-miR-194-3p IGF1R -0.772747277805343 2.9537465170191e-05 -0.704819576268303 0.00353845530141102 MirTarget -0.465153354275548 4.12155219164854e-14 NA NA NA hsa-miR-194-5p IGF1R -0.292530160992458 0.0996141384876663 -0.704819576268303 0.00353845530141102 miRNAWalker2_validate;miRTarBase;miRNATAP -0.583365945764816 5.17802935579868e-20 NA NA NA hsa-miR-204-5p IGF1R -0.537962150012103 0.0330895493358849 -0.704819576268303 0.00353845530141102 mirMAP -0.175246151442439 0.000169572558257274 NA NA NA hsa-miR-33a-3p IGF1R -0.683025859637737 1.01106097756419e-05 -0.704819576268303 0.00353845530141102 miRNATAP -0.24024722365927 0.00153166064129096 NA NA NA hsa-miR-34a-5p IGF1R 1.03694231790865 5.74864019540594e-14 -0.704819576268303 0.00353845530141102 miRNAWalker2_validate -0.278122778996491 0.000818572922363686 NA NA NA hsa-miR-362-3p IGF1R 0.814007335895913 2.00108618836568e-09 -0.704819576268303 0.00353845530141102 PITA -0.261747556128077 0.0022443415729253 NA NA NA hsa-miR-365a-3p IGF1R 0.164591326307714 0.15325084963213 -0.704819576268303 0.00353845530141102 MirTarget -0.509016427701301 6.48840738830501e-07 NA NA NA hsa-miR-374a-5p IGF1R 0.016390363747302 0.869784217438621 -0.704819576268303 0.00353845530141102 mirMAP -0.390232878812442 0.00108904623272643 NA NA NA hsa-miR-378a-3p IGF1R -1.1920055906972 3.23538871733634e-10 -0.704819576268303 0.00353845530141102 miRTarBase;MirTarget;miRNATAP -0.309332132345512 2.97740860751599e-07 24119742;25427715;24412052 The insulin-like growth factor 1 receptor IGF1R was predicted as a potential target of miR-378 and luciferase reporter activity of IGF1R was significantly decreased in the HEK293 cells co-transfected with miR-378 by 41.8% vs the control cells P less than 0.01; miR-378 may suppress growth characteristics of HBV-related HCC by directly targeting the IGF1R 3'-UTR and inhibiting its protein expression;Interestingly members of the miR-378 family presented as a possible target the insulin-like growth factor receptor 1 IGF1R a key signalling molecule in RMS; MiR-378a-3p over-expression in an RMS-derived cell line suppressed IGF1R expression and affected phosphorylated-Akt protein levels;Furthermore miR-378a-3p over-expression or down-regulation could inhibit or enhance insulin-like growth factor 1 receptor IGF1R expression in CRC cells; There was a significantly negative correlation between IGF1R protein expression and miR-378a-3p expression in CRC tissues ;; liver cancer;sarcoma;colorectal cancer hsa-miR-378a-5p IGF1R -1.58667444700188 3.19640857320504e-17 -0.704819576268303 0.00353845530141102 mirMAP -0.264516472073392 1.03932054528896e-05 24119742;25427715 The insulin-like growth factor 1 receptor IGF1R was predicted as a potential target of miR-378 and luciferase reporter activity of IGF1R was significantly decreased in the HEK293 cells co-transfected with miR-378 by 41.8% vs the control cells P less than 0.01; miR-378 may suppress growth characteristics of HBV-related HCC by directly targeting the IGF1R 3'-UTR and inhibiting its protein expression;Interestingly members of the miR-378 family presented as a possible target the insulin-like growth factor receptor 1 IGF1R a key signalling molecule in RMS ; liver cancer;sarcoma hsa-miR-378c IGF1R -1.54028191929038 2.70131554214866e-15 -0.704819576268303 0.00353845530141102 MirTarget;miRNATAP -0.266498982003542 4.51608338557028e-06 NA NA NA hsa-miR-452-5p IGF1R 1.91544498799508 1.81995982853313e-14 -0.704819576268303 0.00353845530141102 mirMAP -0.13514669426121 0.00308740647279285 NA NA NA hsa-miR-455-5p IGF1R -0.270546034117157 0.0581320703577731 -0.704819576268303 0.00353845530141102 miRNATAP -0.33649940148595 4.6083750392036e-05 NA NA NA hsa-miR-500a-3p IGF1R 1.18913245435489 1.59516890341346e-16 -0.704819576268303 0.00353845530141102 mirMAP -0.24207363919467 0.00220221370587418 NA NA NA hsa-miR-532-5p IGF1R 1.02729546510601 9.93318913966636e-16 -0.704819576268303 0.00353845530141102 miRNATAP -0.594000411464035 1.11895616071031e-11 NA NA NA hsa-miR-616-5p IGF1R 0.150569904638223 0.402836556304524 -0.704819576268303 0.00353845530141102 mirMAP -0.341804564452571 1.5998912473218e-07 NA NA NA hsa-miR-885-5p IGF1R -0.940994379567566 0.00118729865914986 -0.704819576268303 0.00353845530141102 PITA;mirMAP -0.156220425611698 0.000105601334784438 NA NA NA hsa-miR-99a-5p IGF1R -1.51481656384839 3.93230057259529e-14 -0.704819576268303 0.00353845530141102 miRNAWalker2_validate;miRTarBase;miRNATAP -0.199500914226479 0.00046535126029909 22425712 Furthermore ectopic transfection of miR-99 family members down-regulated the expression of insulin-like growth factor 1 receptor IGF1R and mechanistic target of rapamycin mTOR genes; The deregulation of miR-99 family contributes to the tumorigenesis of HNSCC in part by targeting IGF1R and mTOR signaling pathways tumorigenesis head and neck cancer hsa-miR-130b-3p IGF2 0.687190305027546 0.000109068658562255 -3.69294069874179 5.99403820984858e-10 mirMAP -0.608939815790691 0.00023785677759273 NA NA NA hsa-miR-193b-5p IGF2 -0.748481053559202 3.37008423124215e-06 -3.69294069874179 5.99403820984858e-10 mirMAP -0.494111714929712 0.00687773918459788 NA NA NA hsa-miR-210-3p IGF2 -0.214021053143938 0.414999515823232 -3.69294069874179 5.99403820984858e-10 MirTarget -0.459707730579746 4.42072409976778e-05 NA NA NA hsa-miR-224-3p IGF2 1.40770116795301 1.31426607144612e-08 -3.69294069874179 5.99403820984858e-10 mirMAP -0.398994767968095 0.000807606270339222 NA NA NA hsa-miR-34a-5p IGF2 1.03694231790865 5.74864019540594e-14 -3.69294069874179 5.99403820984858e-10 mirMAP -1.25726555221864 9.83117679327442e-10 NA NA NA hsa-miR-361-3p IGF2 0.275658012727111 0.00549374744374488 -3.69294069874179 5.99403820984858e-10 mirMAP -0.840984123439573 0.00529861999876599 NA NA NA hsa-miR-491-5p IGF2 -0.369122930844418 0.0154047757048929 -3.69294069874179 5.99403820984858e-10 MirTarget;miRanda -0.541797058750972 0.00597257160148499 NA NA NA hsa-miR-9-5p IGF2 1.26407994492898 8.53156156439881e-05 -3.69294069874179 5.99403820984858e-10 mirMAP -0.449689855202211 6.84845043601393e-07 NA NA NA hsa-miR-125a-5p IKBKG -0.91219969372382 1.38256246489548e-07 0.660366120199928 1.71867865546723e-06 miRanda -0.219029418282295 9.51829321882113e-09 NA NA NA hsa-miR-143-3p IKBKG -0.584123869220553 0.000907217687997436 0.660366120199928 1.71867865546723e-06 mirMAP -0.169551761758995 9.09905571393035e-06 NA NA NA hsa-miR-200a-3p IKBKG -1.49949292595657 3.18410995256133e-05 0.660366120199928 1.71867865546723e-06 mirMAP -0.100439586597671 5.62621816828972e-08 NA NA NA hsa-miR-24-3p IKBKG -0.257825594021966 0.00689742600215061 0.660366120199928 1.71867865546723e-06 mirMAP -0.287544034512102 5.8686253015948e-05 NA NA NA hsa-miR-3065-3p IKBKG -1.04244468016704 4.60743424372577e-05 0.660366120199928 1.71867865546723e-06 mirMAP -0.115721435331344 9.61381024877344e-06 NA NA NA hsa-miR-320a IKBKG 0.325285936317332 0.0221415065838824 0.660366120199928 1.71867865546723e-06 mirMAP -0.182311319218153 0.000133876392194021 NA NA NA hsa-miR-320b IKBKG 0.0919652022571331 0.607981643616945 0.660366120199928 1.71867865546723e-06 mirMAP -0.132285547849802 0.000522961413620221 NA NA NA hsa-miR-335-5p IKBKG -1.61347836513558 1.62757083289711e-19 0.660366120199928 1.71867865546723e-06 mirMAP -0.273399124951207 9.32430291537739e-15 NA NA NA hsa-miR-338-5p IKBKG -0.221898432884726 0.252390988727086 0.660366120199928 1.71867865546723e-06 mirMAP -0.206675822417303 2.68501101428877e-09 NA NA NA hsa-miR-376c-3p IKBKG -1.78544013329054 1.82972185127691e-10 0.660366120199928 1.71867865546723e-06 mirMAP -0.14083607992738 1.85430800686914e-09 NA NA NA hsa-miR-542-3p IKBKG -1.31351015149842 3.64642423288576e-22 0.660366120199928 1.71867865546723e-06 mirMAP -0.292142688932618 4.40119371820177e-10 NA NA NA hsa-miR-107 IL6 0.241662461253461 0.0170788899505389 -2.0069298945737 2.33900822782796e-09 miRanda -0.962941107019514 4.00052775475113e-09 NA NA NA hsa-miR-361-5p IL6 0.233202959858696 0.00961572812255327 -2.0069298945737 2.33900822782796e-09 miRanda -0.746870831233242 6.34041923472147e-05 NA NA NA hsa-miR-365a-3p IL6 0.164591326307714 0.15325084963213 -2.0069298945737 2.33900822782796e-09 miRNAWalker2_validate -0.508496672954192 0.000482261146415095 NA NA NA hsa-let-7e-5p IL6R 0.0350298755544731 0.811068614727804 -0.197357669964791 0.382548993653367 miRNATAP -0.311334738219198 3.31955081908111e-05 NA NA NA hsa-let-7i-5p IL6R -0.14267936165213 0.154141689875847 -0.197357669964791 0.382548993653367 miRNATAP -0.443175862594576 6.10806225986565e-05 NA NA NA hsa-miR-103a-2-5p IL6R 1.17085533927423 5.31418969120877e-09 -0.197357669964791 0.382548993653367 mirMAP -0.15359917006386 0.0067292715186963 NA NA NA hsa-miR-125a-5p IL6R -0.91219969372382 1.38256246489548e-07 -0.197357669964791 0.382548993653367 MirTarget;miRanda -0.238025357559185 0.000137274290397462 NA NA NA hsa-miR-130a-3p IL6R -1.53229181736223 2.91460363624872e-19 -0.197357669964791 0.382548993653367 mirMAP -0.200690702363128 0.00110575859685919 NA NA NA hsa-miR-141-3p IL6R -0.354316381352484 0.256996894288799 -0.197357669964791 0.382548993653367 MirTarget -0.142554332435554 4.93408139054963e-05 NA NA NA hsa-miR-141-5p IL6R -0.499951322764425 0.102654772628289 -0.197357669964791 0.382548993653367 MirTarget -0.117434701677051 0.00140254269544032 NA NA NA hsa-miR-155-5p IL6R 0.0108524225071784 0.95651331033505 -0.197357669964791 0.382548993653367 MirTarget -0.277627367207754 4.05000284880467e-07 NA NA NA hsa-miR-182-5p IL6R 1.96601340710222 4.39326175603633e-09 -0.197357669964791 0.382548993653367 mirMAP -0.141778275229433 9.03668122661657e-06 NA NA NA hsa-miR-200a-3p IL6R -1.49949292595657 3.18410995256133e-05 -0.197357669964791 0.382548993653367 MirTarget -0.159850426034774 9.36193333495953e-08 NA NA NA hsa-miR-21-5p IL6R 1.51084295924322 1.54551622173259e-34 -0.197357669964791 0.382548993653367 MirTarget;miRNATAP -0.536139043119449 2.87865737326418e-11 NA NA NA hsa-miR-23a-3p IL6R -0.178875974460574 0.135978273540604 -0.197357669964791 0.382548993653367 miRNAWalker2_validate;miRTarBase;MirTarget -0.476455604733452 1.77513645727087e-07 NA NA NA hsa-miR-301a-3p IL6R 0.842910511640361 1.43446467417891e-06 -0.197357669964791 0.382548993653367 mirMAP -0.270231870208627 1.2309199906437e-05 NA NA NA hsa-miR-30b-5p IL6R -0.54056186708617 2.44688760798341e-05 -0.197357669964791 0.382548993653367 mirMAP -0.341317440343868 6.08305739490161e-05 NA NA NA hsa-miR-330-3p IL6R -0.332364259424489 0.0316051913061459 -0.197357669964791 0.382548993653367 miRNATAP -0.395044882587144 3.93410619382339e-08 NA NA NA hsa-miR-331-3p IL6R -0.283974346994635 0.0373785411053202 -0.197357669964791 0.382548993653367 MirTarget -0.272591937902894 0.000734050937687351 NA NA NA hsa-miR-421 IL6R 0.941856334694081 6.12167297054077e-08 -0.197357669964791 0.382548993653367 mirMAP -0.184999766620453 0.00310315621875882 NA NA NA hsa-miR-501-5p IL6R 1.15231083477882 2.98906150004088e-10 -0.197357669964791 0.382548993653367 miRNATAP -0.18254438194728 0.00194179348124099 NA NA NA hsa-miR-335-3p IL7 -0.275199247483111 0.106629165248783 -0.477026521881925 0.00101915198923927 mirMAP -0.119073972112747 0.00430087219022835 NA NA NA hsa-let-7e-5p INSR 0.0350298755544731 0.811068614727804 -0.278293402111066 0.00785689311731476 miRNATAP -0.217076706682375 2.52676832104311e-10 NA NA NA hsa-let-7i-5p INSR -0.14267936165213 0.154141689875847 -0.278293402111066 0.00785689311731476 miRNATAP -0.202914490507566 7.59063234403437e-05 NA NA NA hsa-miR-103a-2-5p INSR 1.17085533927423 5.31418969120877e-09 -0.278293402111066 0.00785689311731476 mirMAP -0.143764638707222 2.91318714410811e-08 NA NA NA hsa-miR-140-3p INSR 0.546012239205329 4.84578261098777e-11 -0.278293402111066 0.00785689311731476 mirMAP -0.27738473170784 4.52228010800238e-06 NA NA NA hsa-miR-142-5p INSR -1.44575553250033 1.18797044680592e-13 -0.278293402111066 0.00785689311731476 mirMAP -0.110475843753827 1.18266898108668e-05 NA NA NA hsa-miR-151a-3p INSR 0.330888909161891 0.00418075524865448 -0.278293402111066 0.00785689311731476 miRNATAP -0.115231259307582 0.00938434743719466 NA NA NA hsa-miR-15a-5p INSR 0.349521211668244 0.000770735998804736 -0.278293402111066 0.00785689311731476 miRNATAP -0.251138215261527 2.59574923914891e-07 NA NA NA hsa-miR-15b-3p INSR 0.238105490431193 0.0997721500357416 -0.278293402111066 0.00785689311731476 mirMAP -0.206937351548888 2.77089790710962e-09 NA NA NA hsa-miR-15b-5p INSR 0.230566941413564 0.0824765001857479 -0.278293402111066 0.00785689311731476 miRNATAP -0.186808144180658 1.02375284487422e-06 NA NA NA hsa-miR-16-5p INSR -0.398577235179603 0.000101598495180191 -0.278293402111066 0.00785689311731476 miRNATAP -0.148270888544752 0.00293111089224694 NA NA NA hsa-miR-181a-5p INSR 0.250113435902954 0.0551933249319293 -0.278293402111066 0.00785689311731476 mirMAP -0.196102420475405 4.52506455097139e-07 NA NA NA hsa-miR-181b-5p INSR 0.488256489401108 0.00105321129419701 -0.278293402111066 0.00785689311731476 mirMAP -0.192792987659091 1.02091731606267e-08 NA NA NA hsa-miR-181c-5p INSR -0.00639394152050299 0.969127212255928 -0.278293402111066 0.00785689311731476 mirMAP -0.144920155303009 2.39135053897472e-06 NA NA NA hsa-miR-186-5p INSR -0.060103391373687 0.535288717081205 -0.278293402111066 0.00785689311731476 mirMAP -0.275991289305386 1.62425713237588e-07 NA NA NA hsa-miR-21-5p INSR 1.51084295924322 1.54551622173259e-34 -0.278293402111066 0.00785689311731476 mirMAP -0.133506275914425 0.00045620211593194 24804226;27220494;21618027 Downregulation of miR-21 in radioresistant NSCLC A549 cells inhibited the colony-forming ability and proliferation of A549 cells after IR; Moreover silencing miR-21 enhanced apoptosis of A549 cells induced by IR accompanied by decreased phosphorylated-Akt protein level; However PI3K activator IGF-1 reversed suppression of phosphorylated-Akt protein level and promotion of apoptosis of A549 cells after IR caused by miR-21 knockdown; Silencing miR-21 in radioresistant NSCLC A549 cells sensitized them to IR by inhibiting cell proliferation and enhancing cell apoptosis through inhibition of PI3K/Akt signaling pathway;In addition we also demonstrated that miR-21 confers decreased autophagy in cervical cancer cells after IR via the Akt-mTOR signaling pathway;In this study we found that miR-21 expression was upregulated in response to ionizing radiation IR in U251 cells which suggested that miR-21 could be involved in the response of U251 cells to radiation; We showed that a miR-21 inhibitor enhanced IR-induced glioblastoma cell growth arrest and increased the level of apoptosis which was probably caused by abrogation of the G2-M arrest induced by IR ;;drug resistance lung squamous cell cancer;cervical and endocervical cancer;glioblastoma hsa-miR-27a-3p INSR -0.370807380415112 0.00875721956202713 -0.278293402111066 0.00785689311731476 miRNATAP -0.129448246537712 0.000323191383525876 NA NA NA hsa-miR-32-3p INSR 0.219109440121582 0.207217051619543 -0.278293402111066 0.00785689311731476 mirMAP -0.108738122811479 0.000332795668307009 NA NA NA hsa-miR-330-3p INSR -0.332364259424489 0.0316051913061459 -0.278293402111066 0.00785689311731476 mirMAP -0.105204122509177 0.00182847295060521 NA NA NA hsa-miR-330-5p INSR 0.442231058604099 0.0053308560843808 -0.278293402111066 0.00785689311731476 miRanda -0.124285365243876 0.000101730606261174 NA NA NA hsa-miR-342-3p INSR -0.319677314984262 0.0449813878317147 -0.278293402111066 0.00785689311731476 miRanda -0.16761426288179 1.19136840204847e-07 NA NA NA hsa-miR-361-5p INSR 0.233202959858696 0.00961572812255327 -0.278293402111066 0.00785689311731476 miRanda -0.250639357997155 1.06114867503819e-05 NA NA NA hsa-miR-501-5p INSR 1.15231083477882 2.98906150004088e-10 -0.278293402111066 0.00785689311731476 miRNATAP -0.176376064204615 4.45936602190125e-11 NA NA NA hsa-miR-582-3p INSR -0.653831905315466 0.00248607706603299 -0.278293402111066 0.00785689311731476 mirMAP -0.111253922145084 1.79374163017854e-06 NA NA NA hsa-miR-590-5p INSR -0.103355559980404 0.310027295317092 -0.278293402111066 0.00785689311731476 mirMAP -0.29804167588886 2.22806193728684e-09 NA NA NA hsa-miR-7-1-3p INSR -0.569105143287799 1.85297665041424e-05 -0.278293402111066 0.00785689311731476 mirMAP -0.122187054321131 0.00135939582701306 NA NA NA hsa-miR-1271-5p IRS1 -1.00001044718608 1.30308965099272e-09 -0.221383948238947 0.137544848662312 MirTarget -0.11432442424959 0.00871796439551885 NA NA NA hsa-miR-142-5p IRS1 -1.44575553250033 1.18797044680592e-13 -0.221383948238947 0.137544848662312 MirTarget;PITA -0.225583634781726 1.89537231323331e-10 NA NA NA hsa-miR-143-5p IRS1 -0.42696209881145 0.00646356677356831 -0.221383948238947 0.137544848662312 MirTarget;miRNATAP -0.21309524311581 3.41212250020476e-06 NA NA NA hsa-miR-145-5p IRS1 -1.4795885711477 6.47308361543414e-16 -0.221383948238947 0.137544848662312 miRNAWalker2_validate;miRTarBase;MirTarget -0.14713831440926 0.000110683183121748 22431718;24690171;24762580 Luciferase reporter assay further verified direct target association of miR-145 to specific sites of the IRS1 and IRS2 3'-untranslated regions;MicroRNA 145 suppresses hepatocellular carcinoma by targeting IRS1 and its downstream Akt signaling; We verified IRS1 as a direct target of miR-145 using Western blotting and luciferase reporter assay; Further the restoration of miR-145 in HCC cell lines suppressed cancer cell growth owing to down-regulated IRS1 expression and its downstream Akt/FOXO1 signaling; Our results demonstrated that miR-145 could inhibit HCC through targeting IRS1 and its downstream signaling implicating the loss of miR-145 regulation may be a potential molecular mechanism causing aberrant oncogenic signaling in HCC;IRS-1 was identified as a potential target of miR-145 by dual luciferase reporter assay; Knocking down of IRS-1 had similar effect as overexpression of miR-145 miR-145 might act as a tumor suppressor in uveal melanoma and downregulation of the target IRS-1 might be a potential mechanism ;; liver cancer;liver cancer;melanoma hsa-miR-183-5p IRS1 2.33444328833177 1.52188109365421e-10 -0.221383948238947 0.137544848662312 MirTarget;miRNATAP -0.15378452060776 2.32414530343982e-16 NA NA NA hsa-miR-200b-3p IRS1 -1.29335788099229 0.000268623234305059 -0.221383948238947 0.137544848662312 TargetScan -0.13655932431926 5.29991549499767e-12 26314828 Taken together our results suggest that miR-200 may activate AKT in lung adenocarcinoma cells through a FOG2-independent mechanism involving IRS-1 lung cancer hsa-miR-200c-3p IRS1 -0.104675615392797 0.716957669484981 -0.221383948238947 0.137544848662312 miRNATAP -0.185915259944595 2.49647913870381e-14 NA NA NA hsa-miR-203a-3p IRS1 -1.34362479226226 8.69374456831293e-05 -0.221383948238947 0.137544848662312 MirTarget -0.128445864290196 4.5774299384833e-10 NA NA NA hsa-miR-2355-3p IRS1 -0.471227472721651 0.00718598113738346 -0.221383948238947 0.137544848662312 MirTarget -0.235462938853123 8.40998800532822e-09 NA NA NA hsa-miR-27a-3p IRS1 -0.370807380415112 0.00875721956202713 -0.221383948238947 0.137544848662312 MirTarget;miRNATAP -0.486219830317146 1.62553310998088e-23 NA NA NA hsa-miR-30b-5p IRS1 -0.54056186708617 2.44688760798341e-05 -0.221383948238947 0.137544848662312 MirTarget -0.185037722193096 0.0010185231802762 NA NA NA hsa-miR-335-3p IRS1 -0.275199247483111 0.106629165248783 -0.221383948238947 0.137544848662312 MirTarget -0.215763590579479 3.06379983103195e-07 NA NA NA hsa-miR-338-3p IRS1 0.543135607704581 0.00461167747143826 -0.221383948238947 0.137544848662312 miRanda -0.21579920851113 6.40736021826458e-09 NA NA NA hsa-miR-338-5p IRS1 -0.221898432884726 0.252390988727086 -0.221383948238947 0.137544848662312 PITA;miRNATAP -0.195524165495031 1.51628240629309e-07 NA NA NA hsa-miR-421 IRS1 0.941856334694081 6.12167297054077e-08 -0.221383948238947 0.137544848662312 PITA;miRanda;miRNATAP -0.127783518128362 0.00194370833470413 NA NA NA hsa-miR-429 IRS1 -1.40262535827285 6.93982219171912e-05 -0.221383948238947 0.137544848662312 miRanda;miRNATAP -0.134400612294796 2.94471976691684e-11 NA NA NA hsa-miR-181a-5p ITGA1 0.250113435902954 0.0551933249319293 -0.675046465429795 1.83477212641831e-07 mirMAP -0.159557519521478 0.00116192713555786 NA NA NA hsa-miR-181b-5p ITGA1 0.488256489401108 0.00105321129419701 -0.675046465429795 1.83477212641831e-07 mirMAP -0.17356111081698 4.57364400546711e-05 NA NA NA hsa-miR-21-5p ITGA1 1.51084295924322 1.54551622173259e-34 -0.675046465429795 1.83477212641831e-07 mirMAP -0.324725552717668 3.57684105241524e-12 NA NA NA hsa-miR-30d-3p ITGA1 -0.120562286038759 0.329549534782125 -0.675046465429795 1.83477212641831e-07 MirTarget -0.172229855506642 0.000914088928464785 NA NA NA hsa-miR-32-3p ITGA1 0.219109440121582 0.207217051619543 -0.675046465429795 1.83477212641831e-07 mirMAP -0.132909177760592 0.000465448935685398 NA NA NA hsa-miR-330-3p ITGA1 -0.332364259424489 0.0316051913061459 -0.675046465429795 1.83477212641831e-07 mirMAP -0.11149466710877 0.00847827827544353 NA NA NA hsa-miR-330-5p ITGA1 0.442231058604099 0.0053308560843808 -0.675046465429795 1.83477212641831e-07 miRanda -0.114805585089994 0.00431865400054515 NA NA NA hsa-miR-362-3p ITGA1 0.814007335895913 2.00108618836568e-09 -0.675046465429795 1.83477212641831e-07 miRanda -0.130661452106636 0.00482339454118394 NA NA NA hsa-miR-421 ITGA1 0.941856334694081 6.12167297054077e-08 -0.675046465429795 1.83477212641831e-07 miRanda -0.156977430434281 1.38663519028291e-05 NA NA NA hsa-miR-590-5p ITGA1 -0.103355559980404 0.310027295317092 -0.675046465429795 1.83477212641831e-07 miRanda;mirMAP -0.198886087943981 0.00170748059835869 NA NA NA hsa-miR-107 ITGA10 0.241662461253461 0.0170788899505389 0.643276165652269 0.000377047632206068 miRanda -0.375371458631416 1.86274842179062e-05 NA NA NA hsa-miR-16-5p ITGA10 -0.398577235179603 0.000101598495180191 0.643276165652269 0.000377047632206068 MirTarget -0.269928772403223 0.00181956869041984 NA NA NA hsa-miR-28-5p ITGA10 -0.430964830599964 2.67367257252925e-06 0.643276165652269 0.000377047632206068 MirTarget;miRanda -0.265958680386986 0.00596919596864045 NA NA NA hsa-miR-324-5p ITGA10 0.372933276649782 0.00592280066108904 0.643276165652269 0.000377047632206068 miRanda;miRNATAP -0.185374240181796 0.0046551579841266 NA NA NA hsa-miR-616-5p ITGA10 0.150569904638223 0.402836556304524 0.643276165652269 0.000377047632206068 mirMAP;miRNATAP -0.162408287752269 0.0010133559124676 NA NA NA hsa-miR-532-5p ITGA11 1.02729546510601 9.93318913966636e-16 0.620092456294371 0.0180323746984084 miRNATAP -0.313571288630924 0.00119558249477298 NA NA NA hsa-miR-107 ITGA2 0.241662461253461 0.0170788899505389 1.53120134028147 5.03620431516659e-09 MirTarget;miRanda;miRNATAP -0.595579423085955 3.51813821910157e-06 NA NA NA hsa-miR-130a-3p ITGA2 -1.53229181736223 2.91460363624872e-19 1.53120134028147 5.03620431516659e-09 mirMAP -0.21817609840205 0.00273093457553046 NA NA NA hsa-miR-130b-3p ITGA2 0.687190305027546 0.000109068658562255 1.53120134028147 5.03620431516659e-09 mirMAP -0.238929988104481 0.00100816862732008 NA NA NA hsa-miR-139-5p ITGA2 -2.11299531796557 8.16407076415038e-28 1.53120134028147 5.03620431516659e-09 PITA;miRanda -0.348713430392277 1.86833411063269e-08 NA NA NA hsa-miR-195-5p ITGA2 -1.85907534356434 1.47434041676402e-22 1.53120134028147 5.03620431516659e-09 MirTarget;miRNATAP -0.186383881971086 0.00401127379239066 NA NA NA hsa-miR-19a-3p ITGA2 1.01712596153672 2.57753201450625e-07 1.53120134028147 5.03620431516659e-09 MirTarget -0.232452076582239 0.000333020536473977 NA NA NA hsa-miR-19b-3p ITGA2 0.601279551368824 0.00016685097085251 1.53120134028147 5.03620431516659e-09 MirTarget -0.34350775357453 2.00709629618829e-05 NA NA NA hsa-miR-26b-5p ITGA2 -1.10804856399744 4.14176110314282e-22 1.53120134028147 5.03620431516659e-09 mirMAP -0.455075493018862 2.37547429819346e-05 NA NA NA hsa-miR-27b-3p ITGA2 -0.823491817819754 9.20935302746823e-14 1.53120134028147 5.03620431516659e-09 miRNATAP -0.648959489127879 1.00730961424074e-08 NA NA NA hsa-miR-30c-5p ITGA2 -0.432111642767632 0.000159764714931007 1.53120134028147 5.03620431516659e-09 mirMAP -0.374655382724916 0.000961645145926353 NA NA NA hsa-miR-30e-5p ITGA2 -0.627815042856657 3.02205690184908e-10 1.53120134028147 5.03620431516659e-09 mirMAP -0.737206572787374 6.45205020884361e-09 NA NA NA hsa-miR-326 ITGA2 -1.881935132554 1.68087316863775e-21 1.53120134028147 5.03620431516659e-09 PITA -0.189087919083097 0.00273542798727671 NA NA NA hsa-miR-33a-3p ITGA2 -0.683025859637737 1.01106097756419e-05 1.53120134028147 5.03620431516659e-09 mirMAP -0.480065029116681 5.3570039903457e-09 NA NA NA hsa-miR-3607-3p ITGA2 -2.15518588554493 1.03390728226728e-23 1.53120134028147 5.03620431516659e-09 mirMAP -0.173252523084354 0.0024333016929685 NA NA NA hsa-miR-497-5p ITGA2 -1.41371173021948 7.09278946477743e-17 1.53120134028147 5.03620431516659e-09 MirTarget;miRNATAP -0.293526481317459 6.61553414485539e-05 NA NA NA hsa-miR-139-5p ITGA3 -2.11299531796557 8.16407076415038e-28 0.331189713134492 0.166738416790431 PITA;miRanda -0.174245423626721 0.00198897109798136 NA NA NA hsa-miR-194-3p ITGA3 -0.772747277805343 2.9537465170191e-05 0.331189713134492 0.166738416790431 mirMAP -0.687236365404266 1.70990855825587e-32 NA NA NA hsa-miR-26b-5p ITGA3 -1.10804856399744 4.14176110314282e-22 0.331189713134492 0.166738416790431 miRNAWalker2_validate -0.398707408499467 3.69214242452012e-05 NA NA NA hsa-miR-28-5p ITGA3 -0.430964830599964 2.67367257252925e-06 0.331189713134492 0.166738416790431 miRanda -0.654733430455488 1.9591883294385e-07 NA NA NA hsa-miR-455-5p ITGA3 -0.270546034117157 0.0581320703577731 0.331189713134492 0.166738416790431 miRanda -0.470158293658707 5.65979836634134e-09 NA NA NA hsa-miR-616-3p ITGA3 -0.699516741506971 1.82225985970292e-05 0.331189713134492 0.166738416790431 mirMAP -0.213322177785026 0.00269531236182657 NA NA NA hsa-miR-17-5p ITGA4 0.698861147045461 2.43854106455398e-05 -0.792197018745433 7.52858458455927e-05 TargetScan -0.178764680269899 0.00238821346968674 NA NA NA hsa-miR-362-3p ITGA4 0.814007335895913 2.00108618836568e-09 -0.792197018745433 7.52858458455927e-05 miRanda -0.220497860890506 0.00195524283977063 NA NA NA hsa-miR-455-5p ITGA4 -0.270546034117157 0.0581320703577731 -0.792197018745433 7.52858458455927e-05 miRanda -0.38475521770215 1.5530233128954e-08 NA NA NA hsa-miR-148a-3p ITGA5 -0.748702855596996 3.93313343865641e-07 0.471634888877482 5.29697754708897e-05 miRNATAP -0.307894492928595 8.35158872292536e-17 NA NA NA hsa-miR-32-5p ITGA5 0.0806126674664016 0.548984992497432 0.471634888877482 5.29697754708897e-05 miRNATAP -0.211172885193471 6.1421768497938e-07 NA NA NA hsa-miR-125b-2-3p ITGA6 -1.6609436107315 5.71534047969855e-18 1.33116884445099 5.73067512733422e-21 mirMAP -0.177305489469283 7.67219603417741e-07 NA NA NA hsa-miR-199a-3p ITGA6 -2.3279328685682 1.04468066198593e-17 1.33116884445099 5.73067512733422e-21 PITA -0.166829140227348 2.64008048809431e-11 NA NA NA hsa-miR-199b-3p ITGA6 -2.33226605401203 9.51887435273314e-18 1.33116884445099 5.73067512733422e-21 PITA -0.166957893782338 2.45787733398932e-11 NA NA NA hsa-miR-335-3p ITGA6 -0.275199247483111 0.106629165248783 1.33116884445099 5.73067512733422e-21 mirMAP -0.138340364120149 0.00114101751107001 NA NA NA hsa-miR-342-3p ITGA6 -0.319677314984262 0.0449813878317147 1.33116884445099 5.73067512733422e-21 miRanda -0.124580956342346 0.00625142354926915 NA NA NA hsa-miR-326 ITGA7 -1.881935132554 1.68087316863775e-21 1.11676681367784 2.73009033940488e-11 miRanda -0.161675431886279 6.40430102829214e-05 NA NA NA hsa-miR-30b-5p ITGA8 -0.54056186708617 2.44688760798341e-05 -0.339101571383565 0.266122133539954 mirMAP -0.318911170549572 0.005761661684469 NA NA NA hsa-miR-30d-5p ITGA8 0.718283816348023 2.47422074061352e-08 -0.339101571383565 0.266122133539954 mirMAP -0.296478431997024 0.00929638881203393 NA NA NA hsa-miR-32-3p ITGA8 0.219109440121582 0.207217051619543 -0.339101571383565 0.266122133539954 mirMAP -0.265256602798843 0.00270174304603169 NA NA NA hsa-miR-33a-5p ITGA8 -0.767474460958522 1.09948756118522e-05 -0.339101571383565 0.266122133539954 mirMAP -0.322151827872823 0.000125696352588895 NA NA NA hsa-miR-103a-2-5p ITGA9 1.17085533927423 5.31418969120877e-09 -2.64633266303169 2.99434757393029e-24 mirMAP -0.216380708650452 0.00178582179103033 NA NA NA hsa-miR-194-5p ITGA9 -0.292530160992458 0.0996141384876663 -2.64633266303169 2.99434757393029e-24 miRNAWalker2_validate;miRTarBase -0.292723578816099 0.000106994877245331 NA NA NA hsa-miR-324-5p ITGA9 0.372933276649782 0.00592280066108904 -2.64633266303169 2.99434757393029e-24 miRanda -0.331034329637634 0.000857395121852606 NA NA NA hsa-miR-421 ITGA9 0.941856334694081 6.12167297054077e-08 -2.64633266303169 2.99434757393029e-24 mirMAP -0.328414115699108 1.59728507045812e-05 NA NA NA hsa-miR-452-3p ITGA9 1.76909792952222 2.98172274671408e-10 -2.64633266303169 2.99434757393029e-24 MirTarget -0.246037988671974 1.09858520342469e-07 NA NA NA hsa-miR-452-5p ITGA9 1.91544498799508 1.81995982853313e-14 -2.64633266303169 2.99434757393029e-24 mirMAP -0.297564030308315 6.48304771062172e-09 NA NA NA hsa-miR-501-5p ITGA9 1.15231083477882 2.98906150004088e-10 -2.64633266303169 2.99434757393029e-24 MirTarget -0.257515232969133 0.000345149475279675 NA NA NA hsa-miR-589-3p ITGA9 1.16846704028095 5.88525574424201e-11 -2.64633266303169 2.99434757393029e-24 mirMAP -0.416651601080314 2.07832097094112e-08 NA NA NA hsa-miR-590-5p ITGA9 -0.103355559980404 0.310027295317092 -2.64633266303169 2.99434757393029e-24 miRanda -0.600701170736459 6.02323280840983e-06 NA NA NA hsa-miR-616-5p ITGA9 0.150569904638223 0.402836556304524 -2.64633266303169 2.99434757393029e-24 mirMAP -0.394829399480723 1.06062597733971e-07 NA NA NA hsa-miR-664a-3p ITGA9 0.485807855553031 0.000732553249798598 -2.64633266303169 2.99434757393029e-24 MirTarget -0.277176938495772 0.0029948807533503 NA NA NA hsa-miR-877-5p ITGA9 1.35669625926821 1.43008125755656e-11 -2.64633266303169 2.99434757393029e-24 mirMAP -0.323715679591339 1.79873543711117e-06 NA NA NA hsa-miR-192-5p ITGAV -0.50188499512787 0.00344713341196035 0.42083458357731 0.00760476174317356 miRNAWalker2_validate;MirTarget -0.344370980466671 1.89013250176103e-15 NA NA NA hsa-miR-32-5p ITGAV 0.0806126674664016 0.548984992497432 0.42083458357731 0.00760476174317356 miRNAWalker2_validate;MirTarget;miRNATAP -0.274975166498264 1.42681810268729e-06 NA NA NA hsa-miR-33a-3p ITGAV -0.683025859637737 1.01106097756419e-05 0.42083458357731 0.00760476174317356 MirTarget -0.155984185219414 0.00158654023964867 NA NA NA hsa-miR-92a-3p ITGAV 0.212391012518195 0.134292268089997 0.42083458357731 0.00760476174317356 MirTarget;miRNATAP -0.257814482101981 1.80305424957411e-06 NA NA NA hsa-miR-140-3p ITGB1 0.546012239205329 4.84578261098777e-11 0.150635638369529 0.0875061080940868 PITA -0.175902840174304 0.000564338708599815 NA NA NA hsa-miR-192-5p ITGB1 -0.50188499512787 0.00344713341196035 0.150635638369529 0.0875061080940868 miRNAWalker2_validate -0.159167916481672 6.60024481999039e-11 NA NA NA hsa-miR-590-5p ITGB1 -0.103355559980404 0.310027295317092 0.150635638369529 0.0875061080940868 miRanda -0.136906492054453 0.00125518477546468 NA NA NA hsa-miR-146b-5p ITGB3 0.419719546795449 0.0457375823220155 -0.984722780911358 0.000225186373225592 miRanda -0.209405714984689 0.000753494487303087 NA NA NA hsa-miR-221-3p ITGB3 1.12244756358612 3.48370308238012e-13 -0.984722780911358 0.000225186373225592 MirTarget -0.386235530184791 2.395985878903e-06 NA NA NA hsa-miR-222-3p ITGB3 1.08574156066353 1.76407192348131e-11 -0.984722780911358 0.000225186373225592 MirTarget -0.292691325646809 0.000210297407328954 NA NA NA hsa-miR-30b-5p ITGB3 -0.54056186708617 2.44688760798341e-05 -0.984722780911358 0.000225186373225592 mirMAP -0.338348015277282 0.000871538256799819 20498642 Enforced constitutive expression of mir-30 in BT-ICs inhibits their self-renewal capacity by reducing Ubc9 and induces apoptosis through silencing ITGB3 breast cancer hsa-miR-30d-5p ITGB3 0.718283816348023 2.47422074061352e-08 -0.984722780911358 0.000225186373225592 miRNATAP -0.536944941610996 6.146536518821e-08 NA NA NA hsa-miR-338-3p ITGB3 0.543135607704581 0.00461167747143826 -0.984722780911358 0.000225186373225592 miRanda -0.215934453214511 0.0015046418079117 27431198 Furthermore we also identified a metastasis related protein integrin β3 ITGB3 as a novel target gene of miR-338 metastasis lung cancer hsa-miR-429 ITGB3 -1.40262535827285 6.93982219171912e-05 -0.984722780911358 0.000225186373225592 miRanda -0.108897560094347 0.00347304092504122 NA NA NA hsa-miR-378a-5p ITGB5 -1.58667444700188 3.19640857320504e-17 0.292212681922662 0.00246071719815647 MirTarget -0.121968371154877 2.94684418151273e-07 NA NA NA hsa-miR-486-5p ITGB5 -1.77766890600022 2.9071717929922e-14 0.292212681922662 0.00246071719815647 miRanda -0.117509353238263 8.00849793913438e-10 NA NA NA hsa-miR-130b-3p ITGB8 0.687190305027546 0.000109068658562255 -2.87006066676904 1.87352126528694e-11 MirTarget;miRNATAP -0.423990971522566 0.000376568737727015 NA NA NA hsa-miR-148a-3p ITGB8 -0.748702855596996 3.93313343865641e-07 -2.87006066676904 1.87352126528694e-11 miRNATAP -1.14663315826581 1.03967657771964e-16 NA NA NA hsa-miR-17-5p ITGB8 0.698861147045461 2.43854106455398e-05 -2.87006066676904 1.87352126528694e-11 MirTarget;TargetScan;miRNATAP -0.509781440182782 6.36953028073789e-05 NA NA NA hsa-miR-19a-3p ITGB8 1.01712596153672 2.57753201450625e-07 -2.87006066676904 1.87352126528694e-11 MirTarget -0.314178137932746 0.00323146738841454 NA NA NA hsa-miR-19b-3p ITGB8 0.601279551368824 0.00016685097085251 -2.87006066676904 1.87352126528694e-11 MirTarget -0.397676292718566 0.00280329871319981 NA NA NA hsa-miR-20a-5p ITGB8 0.854326758727003 5.04256669663285e-07 -2.87006066676904 1.87352126528694e-11 MirTarget;miRNATAP -0.630843134227604 2.80236468955331e-07 NA NA NA hsa-miR-32-3p ITGB8 0.219109440121582 0.207217051619543 -2.87006066676904 1.87352126528694e-11 MirTarget -0.422776705495879 0.000876390100110617 NA NA NA hsa-miR-361-5p ITGB8 0.233202959858696 0.00961572812255327 -2.87006066676904 1.87352126528694e-11 miRanda -0.896687736206458 0.00017672463671778 NA NA NA hsa-miR-3682-3p ITGB8 0.805600143458555 8.7874424633131e-06 -2.87006066676904 1.87352126528694e-11 MirTarget -0.315252610334304 0.00973503314126187 NA NA NA hsa-miR-532-5p ITGB8 1.02729546510601 9.93318913966636e-16 -2.87006066676904 1.87352126528694e-11 PITA -1.18521452547631 4.64583207595488e-14 NA NA NA hsa-miR-616-5p ITGB8 0.150569904638223 0.402836556304524 -2.87006066676904 1.87352126528694e-11 MirTarget -0.543654869257247 4.36895818081019e-06 NA NA NA hsa-miR-93-5p ITGB8 1.39518349670953 2.84167728726712e-25 -2.87006066676904 1.87352126528694e-11 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.650748696121705 1.28958792274442e-05 NA NA NA hsa-miR-106b-5p JAK1 0.648901865047099 8.24870862047885e-09 -0.491502741052473 2.21647945606145e-08 miRNAWalker2_validate;MirTarget -0.274241391983959 1.386588824919e-13 NA NA NA hsa-miR-128-3p JAK1 0.289735108018712 0.0114419474207889 -0.491502741052473 2.21647945606145e-08 miRNAWalker2_validate -0.109369528499963 0.00404231658945177 NA NA NA hsa-miR-17-5p JAK1 0.698861147045461 2.43854106455398e-05 -0.491502741052473 2.21647945606145e-08 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.268052325740918 3.25515675433912e-28 NA NA NA hsa-miR-20a-5p JAK1 0.854326758727003 5.04256669663285e-07 -0.491502741052473 2.21647945606145e-08 MirTarget -0.23386116447226 1.56734608485868e-22 NA NA NA hsa-miR-30d-5p JAK1 0.718283816348023 2.47422074061352e-08 -0.491502741052473 2.21647945606145e-08 MirTarget -0.145093822003298 1.12748945024431e-05 NA NA NA hsa-miR-455-5p JAK1 -0.270546034117157 0.0581320703577731 -0.491502741052473 2.21647945606145e-08 miRanda -0.167977882302863 2.26382130466332e-08 NA NA NA hsa-miR-93-5p JAK1 1.39518349670953 2.84167728726712e-25 -0.491502741052473 2.21647945606145e-08 MirTarget;miRNATAP -0.238027881672368 6.65509610107069e-16 NA NA NA hsa-miR-139-5p JAK3 -2.11299531796557 8.16407076415038e-28 -0.0341347925675266 0.875605617465597 miRanda -0.183410063739571 0.000335328682413109 NA NA NA hsa-miR-30b-3p JAK3 -0.439724365151373 0.000947180503583586 -0.0341347925675266 0.875605617465597 MirTarget -0.298822167939758 0.000168370600584498 NA NA NA hsa-miR-616-5p JAK3 0.150569904638223 0.402836556304524 -0.0341347925675266 0.875605617465597 MirTarget -0.275006451227061 2.84326544589298e-06 NA NA NA hsa-miR-149-5p KDR -0.317176971910036 0.187207559733877 -0.806222400057247 9.66990863598643e-06 miRNATAP -0.161937905853182 1.36302184666799e-05 NA NA NA hsa-miR-15a-5p KDR 0.349521211668244 0.000770735998804736 -0.806222400057247 9.66990863598643e-06 miRNATAP -0.372756033627348 1.49275800717172e-05 NA NA NA hsa-miR-15b-5p KDR 0.230566941413564 0.0824765001857479 -0.806222400057247 9.66990863598643e-06 miRNATAP -0.3391651854872 4.33957562686082e-07 NA NA NA hsa-miR-200c-3p KDR -0.104675615392797 0.716957669484981 -0.806222400057247 9.66990863598643e-06 miRNATAP -0.116520342266903 0.00016541089831424 24205206 MiR 200c increases the radiosensitivity of non small cell lung cancer cell line A549 by targeting VEGF VEGFR2 pathway; MiR-200c at the nexus of epithelial-mesenchymal transition EMT is predicted to target VEGFR2; The purpose of this study is to test the hypothesis that regulation of VEGFR2 pathway by miR-200c could modulate the radiosensitivity of cancer cells; Bioinformatic analysis luciferase reporter assays and biochemical assays were carried out to validate VEGFR2 as a direct target of miR-200c; We identified VEGFR2 as a novel target of miR-200c lung squamous cell cancer hsa-miR-338-3p KDR 0.543135607704581 0.00461167747143826 -0.806222400057247 9.66990863598643e-06 PITA;miRanda -0.124638389203913 0.00756237946861911 NA NA NA hsa-miR-590-5p KDR -0.103355559980404 0.310027295317092 -0.806222400057247 9.66990863598643e-06 miRanda -0.641615310224899 1.28069025499026e-13 NA NA NA hsa-miR-618 KDR 0.13539895350352 0.517145913114103 -0.806222400057247 9.66990863598643e-06 PITA -0.135808208561215 0.00224740471076939 NA NA NA hsa-miR-128-3p KIT 0.289735108018712 0.0114419474207889 -0.36947956147857 0.0945270050690569 MirTarget -0.342258520102763 0.000278760044005399 NA NA NA hsa-miR-130b-3p KIT 0.687190305027546 0.000109068658562255 -0.36947956147857 0.0945270050690569 miRNATAP -0.248535529800642 3.36822677046523e-05 NA NA NA hsa-miR-193a-3p KIT -0.117675551589896 0.309391958208578 -0.36947956147857 0.0945270050690569 MirTarget;PITA;miRanda;miRNATAP -0.332272363921644 0.000380762841679538 NA NA NA hsa-miR-193b-3p KIT -0.166697207281748 0.272022543069482 -0.36947956147857 0.0945270050690569 MirTarget;miRNATAP -0.324351670932747 4.42836626546931e-06 NA NA NA hsa-miR-20a-5p KIT 0.854326758727003 5.04256669663285e-07 -0.36947956147857 0.0945270050690569 miRTarBase -0.225765388624807 0.000296472897214121 NA NA NA hsa-miR-192-3p KITLG -0.6401165114007 0.000266272092403814 0.795506881904605 2.04054574930174e-05 mirMAP -0.295004448161722 8.59654669637728e-09 NA NA NA hsa-miR-23b-3p KITLG -0.530561587109023 5.43807206823652e-07 0.795506881904605 2.04054574930174e-05 mirMAP -0.348544188357782 4.92671720566124e-05 NA NA NA hsa-miR-26b-5p KITLG -1.10804856399744 4.14176110314282e-22 0.795506881904605 2.04054574930174e-05 mirMAP -0.234810293820746 0.00211032519308696 NA NA NA hsa-miR-33a-3p KITLG -0.683025859637737 1.01106097756419e-05 0.795506881904605 2.04054574930174e-05 mirMAP -0.224106371999546 0.00013598203009133 NA NA NA hsa-miR-345-5p KITLG -0.712725883885838 3.60393522979373e-06 0.795506881904605 2.04054574930174e-05 MirTarget -0.156313293057969 0.00827560779339111 NA NA NA hsa-miR-140-3p KRAS 0.546012239205329 4.84578261098777e-11 -0.338081744192936 0.00043842779003105 MirTarget -0.247198429138234 9.22756583574077e-06 NA NA NA hsa-miR-16-1-3p KRAS 0.392360028038559 0.00112481918108198 -0.338081744192936 0.00043842779003105 mirMAP -0.114704330599848 0.00330327136149234 NA NA NA hsa-miR-193a-3p KRAS -0.117675551589896 0.309391958208578 -0.338081744192936 0.00043842779003105 MirTarget;miRanda;miRNATAP -0.128182356310374 0.00170700856743147 NA NA NA hsa-miR-193b-3p KRAS -0.166697207281748 0.272022543069482 -0.338081744192936 0.00043842779003105 miRNAWalker2_validate;MirTarget;miRNATAP -0.120510531869791 9.78248377856047e-05 25905463 Deregulation of the MiR 193b KRAS Axis Contributes to Impaired Cell Growth in Pancreatic Cancer; Mechanistically KRAS was verified as a direct effector of miR-193b through which the AKT and ERK pathways were modulated and cell growth of PDAC cells was suppressed; Taken together our findings indicate that miR-193b-mediated deregulation of the KRAS axis is involved in pancreatic carcinogenesis and suggest that miR-193b could be a potentially effective target for PDAC therapy tumorigenesis pancreatic cancer hsa-miR-30d-3p KRAS -0.120562286038759 0.329549534782125 -0.338081744192936 0.00043842779003105 MirTarget;miRNATAP -0.132565215112955 0.000512493362671086 NA NA NA hsa-miR-532-5p KRAS 1.02729546510601 9.93318913966636e-16 -0.338081744192936 0.00043842779003105 MirTarget;PITA;miRNATAP -0.137739612561571 0.000100604507963156 NA NA NA hsa-miR-125a-3p LAMA3 -0.841683158494395 3.51202687176603e-05 1.44669397870991 3.68334239314585e-06 miRanda -0.23032131162349 0.00271226730803684 NA NA NA hsa-miR-17-5p LAMA3 0.698861147045461 2.43854106455398e-05 1.44669397870991 3.68334239314585e-06 TargetScan -0.48787800614806 8.9429862836022e-08 NA NA NA hsa-miR-181a-5p LAMA3 0.250113435902954 0.0551933249319293 1.44669397870991 3.68334239314585e-06 miRNAWalker2_validate -0.480393838339496 4.68441145042477e-05 NA NA NA hsa-miR-217 LAMA3 1.05797297540381 0.0401026692682422 1.44669397870991 3.68334239314585e-06 PITA;miRanda -0.141216744729264 1.72158329960106e-06 NA NA NA hsa-miR-32-3p LAMA4 0.219109440121582 0.207217051619543 1.54113543763468 7.58762192757364e-27 MirTarget -0.120034460753502 0.0070055231506092 NA NA NA hsa-miR-107 LAMA5 0.241662461253461 0.0170788899505389 0.766399752598965 4.15234329403643e-06 miRanda -0.311255712969823 0.000127997930240277 NA NA NA hsa-miR-193b-3p LAMB1 -0.166697207281748 0.272022543069482 0.253755164977762 0.153374738534835 miRNAWalker2_validate -0.329353493273974 5.052659610824e-09 NA NA NA hsa-miR-484 LAMB2 0.0857240033709656 0.453982056282229 -0.189674019369094 0.13994379902454 miRNAWalker2_validate -0.196811435218037 0.000338018939372097 NA NA NA hsa-miR-122-5p LAMC1 -1.23907441373955 4.42181244888642e-06 1.18164580989765 3.65525462453826e-17 MirTarget;miRNATAP -0.212671515224247 2.05957517770403e-17 NA NA NA hsa-miR-139-5p LAMC1 -2.11299531796557 8.16407076415038e-28 1.18164580989765 3.65525462453826e-17 miRanda -0.226258385144221 1.69064747798937e-11 NA NA NA hsa-miR-193a-3p LAMC1 -0.117675551589896 0.309391958208578 1.18164580989765 3.65525462453826e-17 MirTarget;PITA;miRanda -0.197168799954626 0.00142654058419805 NA NA NA hsa-miR-193b-3p LAMC1 -0.166697207281748 0.272022543069482 1.18164580989765 3.65525462453826e-17 MirTarget -0.230227935704299 7.58927130756756e-07 NA NA NA hsa-miR-195-5p LAMC1 -1.85907534356434 1.47434041676402e-22 1.18164580989765 3.65525462453826e-17 MirTarget -0.160738646500811 4.77301456025353e-06 NA NA NA hsa-miR-22-3p LAMC1 -0.628561621952407 1.33755558015523e-09 1.18164580989765 3.65525462453826e-17 MirTarget;miRNATAP -0.444929117565246 2.00326518461981e-11 26052614 The identification of laminin gamma 1 LAMC1 and myeloid cell leukemia 1 MCL1 as direct targets of miR-22 and miR-29a respectively suggested a tumor-suppressive role of these miRNAs prostate cancer hsa-miR-29a-3p LAMC1 -0.856762511178136 4.65330336378459e-12 1.18164580989765 3.65525462453826e-17 MirTarget;miRNATAP -0.293512586709957 1.24062705212149e-07 26052614 The identification of laminin gamma 1 LAMC1 and myeloid cell leukemia 1 MCL1 as direct targets of miR-22 and miR-29a respectively suggested a tumor-suppressive role of these miRNAs prostate cancer hsa-miR-29c-3p LAMC1 -1.4367517608807 1.61767774905781e-21 1.18164580989765 3.65525462453826e-17 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.247557685092554 2.2318434063202e-08 NA NA NA hsa-miR-33a-3p LAMC1 -0.683025859637737 1.01106097756419e-05 1.18164580989765 3.65525462453826e-17 mirMAP -0.127691716393828 0.00517308713713168 NA NA NA hsa-miR-424-5p LAMC1 -2.62653257498508 2.38997974737991e-50 1.18164580989765 3.65525462453826e-17 MirTarget;miRNATAP -0.164700621655061 3.21163281537882e-06 NA NA NA hsa-miR-497-5p LAMC1 -1.41371173021948 7.09278946477743e-17 1.18164580989765 3.65525462453826e-17 MirTarget;miRNATAP -0.158099601835923 8.46733346137645e-05 NA NA NA hsa-miR-548b-3p LAMC1 -0.0203576084717134 0.930819503166956 1.18164580989765 3.65525462453826e-17 PITA -0.101818565132175 0.000912887751443203 NA NA NA hsa-miR-7-1-3p LAMC1 -0.569105143287799 1.85297665041424e-05 1.18164580989765 3.65525462453826e-17 mirMAP -0.165571379361951 0.00184705996977273 NA NA NA hsa-miR-148b-3p LAMC2 0.266862251671911 0.00185271302045935 -1.88411646680975 6.65805574231518e-06 miRNAWalker2_validate -0.825518499166096 0.000668242286477737 NA NA NA hsa-miR-193b-3p LAMC2 -0.166697207281748 0.272022543069482 -1.88411646680975 6.65805574231518e-06 MirTarget;miRNATAP -0.688371243499296 3.2964291900371e-07 NA NA NA hsa-miR-548b-3p LAMC2 -0.0203576084717134 0.930819503166956 -1.88411646680975 6.65805574231518e-06 miRNATAP -0.727182288278947 2.50684183944719e-17 NA NA NA hsa-miR-660-5p LAMC2 0.989755850734071 5.69930767464937e-14 -1.88411646680975 6.65805574231518e-06 MirTarget -0.743765339410472 8.10222595132239e-07 NA NA NA hsa-miR-130b-3p LAMC3 0.687190305027546 0.000109068658562255 -1.16336935602705 0.000136555756942437 mirMAP -0.406225489356001 9.8471707300354e-07 NA NA NA hsa-miR-192-3p LAMC3 -0.6401165114007 0.000266272092403814 -1.16336935602705 0.000136555756942437 mirMAP -0.2707235641559 0.00139897549549915 NA NA NA hsa-miR-192-5p LAMC3 -0.50188499512787 0.00344713341196035 -1.16336935602705 0.000136555756942437 mirMAP -0.298507199873094 0.00059027334318161 NA NA NA hsa-miR-301a-3p LAMC3 0.842910511640361 1.43446467417891e-06 -1.16336935602705 0.000136555756942437 mirMAP -0.224293685945599 0.00812169480621829 NA NA NA hsa-miR-324-3p LAMC3 0.260348469146494 0.0506141704203545 -1.16336935602705 0.000136555756942437 mirMAP -0.338384884053783 0.0026971745450545 NA NA NA hsa-miR-425-5p LAMC3 0.585974239943864 2.23063410931402e-05 -1.16336935602705 0.000136555756942437 mirMAP -0.481176991949151 6.9025100338245e-06 NA NA NA hsa-miR-452-5p LAMC3 1.91544498799508 1.81995982853313e-14 -1.16336935602705 0.000136555756942437 mirMAP -0.178774038812905 0.00199619319790811 NA NA NA hsa-miR-548b-3p LAMC3 -0.0203576084717134 0.930819503166956 -1.16336935602705 0.000136555756942437 mirMAP -0.318659553565364 6.77627639103087e-07 NA NA NA hsa-miR-32-3p LPAR1 0.219109440121582 0.207217051619543 -2.13909010529734 2.75317798314012e-10 mirMAP -0.281633373050648 0.00512753947643752 NA NA NA hsa-miR-361-5p LPAR1 0.233202959858696 0.00961572812255327 -2.13909010529734 2.75317798314012e-10 miRanda -0.783455322312021 3.27747341991044e-05 NA NA NA hsa-miR-374a-5p LPAR1 0.016390363747302 0.869784217438621 -2.13909010529734 2.75317798314012e-10 MirTarget -0.596072873916971 0.000474674061540556 NA NA NA hsa-miR-664a-3p LPAR1 0.485807855553031 0.000732553249798598 -2.13909010529734 2.75317798314012e-10 mirMAP -0.30676192900774 0.00889862811070262 NA NA NA hsa-miR-28-5p LPAR3 -0.430964830599964 2.67367257252925e-06 0.124739277242714 0.693482502223957 miRanda -0.512336436722084 0.00227955534968955 NA NA NA hsa-miR-365a-3p LPAR5 0.164591326307714 0.15325084963213 -0.86446583840413 1.15354228230199e-05 MirTarget -0.402313291245856 1.68497210970912e-06 NA NA NA hsa-miR-140-3p MAP2K1 0.546012239205329 4.84578261098777e-11 -1.22814648702538 4.27448032410174e-41 PITA -0.381987666476187 3.26697824928144e-11 NA NA NA hsa-miR-34a-5p MAP2K1 1.03694231790865 5.74864019540594e-14 -1.22814648702538 4.27448032410174e-41 miRNAWalker2_validate;miRTarBase -0.229470281840033 1.32655977381036e-11 NA NA NA hsa-miR-361-5p MAP2K1 0.233202959858696 0.00961572812255327 -1.22814648702538 4.27448032410174e-41 miRanda -0.180575442044722 0.00107072926955335 NA NA NA hsa-miR-501-5p MAP2K1 1.15231083477882 2.98906150004088e-10 -1.22814648702538 4.27448032410174e-41 PITA -0.144135390798289 3.07397683406163e-08 NA NA NA hsa-let-7b-5p MAP2K2 -0.959013717270803 5.95127281258643e-11 0.626002935787801 2.22116323165311e-10 miRNAWalker2_validate -0.179048006528372 3.03569472992354e-08 NA NA NA hsa-miR-106b-5p MCL1 0.648901865047099 8.24870862047885e-09 -1.08478249996404 1.88833352070372e-27 MirTarget;miRNATAP -0.233931018512188 2.1829727586773e-07 NA NA NA hsa-miR-15a-5p MCL1 0.349521211668244 0.000770735998804736 -1.08478249996404 1.88833352070372e-27 miRNAWalker2_validate -0.144477692829072 0.00399514264598915 NA NA NA hsa-miR-20a-5p MCL1 0.854326758727003 5.04256669663285e-07 -1.08478249996404 1.88833352070372e-27 MirTarget;miRNATAP -0.114471714846913 0.000140895848956066 23594563 Subsequent investigations revealed that miR-20a directly targeted myeloid cell leukemia sequence 1 Mcl-1 and reduced the endogenous protein level of Mcl-1 in HCC cells liver cancer hsa-miR-361-5p MCL1 0.233202959858696 0.00961572812255327 -1.08478249996404 1.88833352070372e-27 miRanda -0.158404779852559 0.00664587993430657 NA NA NA hsa-miR-589-3p MCL1 1.16846704028095 5.88525574424201e-11 -1.08478249996404 1.88833352070372e-27 MirTarget -0.139830453535339 1.2143037200312e-06 NA NA NA hsa-miR-93-5p MCL1 1.39518349670953 2.84167728726712e-25 -1.08478249996404 1.88833352070372e-27 MirTarget;miRNATAP -0.273783622385872 1.05555530350771e-14 NA NA NA hsa-miR-148b-5p MDM2 0.295777213389605 0.0255749859535438 -0.36172823237465 0.000468851696238999 mirMAP -0.126111944341174 0.000979992057303969 NA NA NA hsa-miR-181a-2-3p MDM2 -0.232005557505191 0.236404981276858 -0.36172823237465 0.000468851696238999 mirMAP -0.112388243613946 1.17734645194124e-05 NA NA NA hsa-miR-23a-3p MDM2 -0.178875974460574 0.135978273540604 -0.36172823237465 0.000468851696238999 mirMAP -0.134193900580038 0.00153318263298883 NA NA NA hsa-miR-25-3p MDM2 0.633062563556379 3.68686354834328e-10 -0.36172823237465 0.000468851696238999 miRNAWalker2_validate;miRTarBase -0.177207631206492 0.0003251640137327 NA NA NA hsa-miR-500a-5p MDM2 0.797906382969156 9.15958171084226e-08 -0.36172823237465 0.000468851696238999 mirMAP -0.143618881374049 1.52638427528629e-05 NA NA NA hsa-miR-589-3p MDM2 1.16846704028095 5.88525574424201e-11 -0.36172823237465 0.000468851696238999 mirMAP -0.123089784785846 1.18949322878182e-05 NA NA NA hsa-miR-125a-3p MET -0.841683158494395 3.51202687176603e-05 -0.153206483336303 0.252348462860111 miRanda -0.156151209622555 1.20437354901061e-06 NA NA NA hsa-miR-130a-3p MET -1.53229181736223 2.91460363624872e-19 -0.153206483336303 0.252348462860111 miRNAWalker2_validate -0.174282381687757 1.40093229108985e-06 NA NA NA hsa-miR-324-5p MET 0.372933276649782 0.00592280066108904 -0.153206483336303 0.252348462860111 miRanda -0.265570825799172 2.14440923054991e-08 NA NA NA hsa-miR-362-3p MET 0.814007335895913 2.00108618836568e-09 -0.153206483336303 0.252348462860111 miRanda -0.144789460757098 0.00214250548628117 NA NA NA hsa-miR-421 MET 0.941856334694081 6.12167297054077e-08 -0.153206483336303 0.252348462860111 miRanda -0.12059316211551 0.00110497994976015 NA NA NA hsa-miR-126-5p MTCP1 -0.428561469405594 6.6035585844222e-05 -0.522159393260238 0.00195658202473798 mirMAP -0.225000869144181 0.00339413297682873 NA NA NA hsa-miR-17-3p MTCP1 0.410352776142368 0.00422200500748636 -0.522159393260238 0.00195658202473798 MirTarget -0.162274535152001 0.00477066598810189 NA NA NA hsa-miR-181b-5p MTOR 0.488256489401108 0.00105321129419701 0.127337008584315 0.234493252196033 miRNAWalker2_validate -0.222677068776147 6.82613635172636e-11 NA NA NA hsa-miR-744-5p MTOR -0.378868377317939 0.00125071229180016 0.127337008584315 0.234493252196033 miRNAWalker2_validate -0.166050890940013 0.000174602508440508 NA NA NA hsa-miR-101-3p MYB -1.48176407980989 3.98950707385704e-31 0.832619773672674 0.00191922988334194 miRNATAP -0.543316757987601 8.23206412612299e-09 NA NA NA hsa-miR-139-5p MYB -2.11299531796557 8.16407076415038e-28 0.832619773672674 0.00191922988334194 miRanda -0.436319209735813 1.8913506713086e-12 NA NA NA hsa-miR-144-3p MYB -2.97536914478815 6.00546047309683e-28 0.832619773672674 0.00191922988334194 miRNATAP -0.127402796229373 0.00475510455309564 NA NA NA hsa-miR-193b-3p MYB -0.166697207281748 0.272022543069482 0.832619773672674 0.00191922988334194 miRNAWalker2_validate -0.245398071034016 0.00472658029717421 NA NA NA hsa-miR-34a-5p MYB 1.03694231790865 5.74864019540594e-14 0.832619773672674 0.00191922988334194 miRNAWalker2_validate;miRTarBase -0.28113576142756 0.00236602949553086 NA NA NA hsa-miR-423-5p MYC 0.698807775906485 1.02195196334612e-11 -1.53204423111148 3.53987625613016e-10 miRNAWalker2_validate -0.328206409340035 0.00484551582134482 NA NA NA hsa-miR-15a-5p NFKB1 0.349521211668244 0.000770735998804736 -0.538916955748098 1.42993670239858e-08 miRNAWalker2_validate -0.153048958379264 0.000730484492411411 NA NA NA hsa-miR-548j-5p NFKB1 0.251239647701947 0.171361975450222 -0.538916955748098 1.42993670239858e-08 MirTarget -0.106885227436574 4.88574003092244e-05 NA NA NA hsa-miR-92a-3p NFKB1 0.212391012518195 0.134292268089997 -0.538916955748098 1.42993670239858e-08 miRNAWalker2_validate -0.147016189975437 8.47280185007855e-06 NA NA NA hsa-miR-423-5p NGF 0.698807775906485 1.02195196334612e-11 -1.13456502234037 7.24473953628721e-08 MirTarget;miRNATAP -0.447502128127762 6.35933337883299e-06 NA NA NA hsa-miR-10b-5p NGFR 2.86931593037303 6.97409423207806e-23 -3.21029001367102 1.44390076140831e-24 mirMAP -0.397416303249616 6.91420749264163e-15 NA NA NA hsa-miR-15b-3p NGFR 0.238105490431193 0.0997721500357416 -3.21029001367102 1.44390076140831e-24 mirMAP -0.50964422238366 5.10367178136392e-06 NA NA NA hsa-miR-34a-5p NGFR 1.03694231790865 5.74864019540594e-14 -3.21029001367102 1.44390076140831e-24 mirMAP -0.558348892128834 8.29526099358313e-07 NA NA NA hsa-miR-324-5p NR4A1 0.372933276649782 0.00592280066108904 -2.32431301974999 3.6888067569463e-24 miRanda -0.390166540555856 7.11244870350508e-06 NA NA NA hsa-miR-361-5p NR4A1 0.233202959858696 0.00961572812255327 -2.32431301974999 3.6888067569463e-24 miRanda -0.481298783579483 0.000284512300406185 NA NA NA hsa-miR-93-5p NR4A1 1.39518349670953 2.84167728726712e-25 -2.32431301974999 3.6888067569463e-24 miRNAWalker2_validate -0.710204481874521 5.77807530080087e-19 NA NA NA hsa-let-7b-5p NRAS -0.959013717270803 5.95127281258643e-11 0.301156333096785 0.000285671613438218 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.105903409272711 8.57245668973746e-05 20607356 Transfection of let-7 miRNA reduced expression of pan-RAS N-RAS and K-RAS in the glioblastoma cells glioblastoma hsa-miR-126-5p NRAS -0.428561469405594 6.6035585844222e-05 0.301156333096785 0.000285671613438218 mirMAP -0.145651073417946 0.000106824688349933 NA NA NA hsa-miR-145-5p NRAS -1.4795885711477 6.47308361543414e-16 0.301156333096785 0.000285671613438218 miRNAWalker2_validate;MirTarget;miRNATAP -0.120395847533196 1.03194548202048e-08 26973415 miR-145 expression was significantly downregulated in colon cancer tissues with its expression in normal colonic tissues being 4-5-fold higher two sample t test P < 0.05 whereas N-ras expression showed the opposite trend colon cancer hsa-miR-22-3p NRAS -0.628561621952407 1.33755558015523e-09 0.301156333096785 0.000285671613438218 MirTarget;miRNATAP -0.287363482934569 1.84836871548684e-14 NA NA NA hsa-miR-29a-3p NRAS -0.856762511178136 4.65330336378459e-12 0.301156333096785 0.000285671613438218 miRNATAP -0.111209986118726 0.000497462508043792 NA NA NA hsa-miR-29b-3p NRAS -0.348885528720517 0.0121441341291285 0.301156333096785 0.000285671613438218 miRNATAP -0.163441778679427 1.31476396622707e-08 NA NA NA hsa-miR-29c-3p NRAS -1.4367517608807 1.61767774905781e-21 0.301156333096785 0.000285671613438218 miRNATAP -0.195416271463693 3.04370398757482e-15 NA NA NA hsa-miR-664a-3p NRAS 0.485807855553031 0.000732553249798598 0.301156333096785 0.000285671613438218 MirTarget -0.123577371649095 9.58480979576424e-06 NA NA NA hsa-miR-21-5p NTF3 1.51084295924322 1.54551622173259e-34 -3.97962534066229 2.44784627289438e-41 miRTarBase;miRNATAP -1.02982933864977 2.33503442659123e-19 NA NA NA hsa-miR-221-3p NTF3 1.12244756358612 3.48370308238012e-13 -3.97962534066229 2.44784627289438e-41 miRNATAP -0.487132834663555 1.03773554125066e-06 NA NA NA hsa-miR-222-3p NTF3 1.08574156066353 1.76407192348131e-11 -3.97962534066229 2.44784627289438e-41 miRNATAP -0.44960074696525 2.69176815020579e-06 NA NA NA hsa-miR-421 NTF3 0.941856334694081 6.12167297054077e-08 -3.97962534066229 2.44784627289438e-41 miRanda -0.509139650144783 1.28862404044202e-08 NA NA NA hsa-miR-590-5p NTF3 -0.103355559980404 0.310027295317092 -3.97962534066229 2.44784627289438e-41 PITA;miRanda;miRNATAP -0.438231227038025 0.00572599858717309 NA NA NA hsa-miR-107 NTRK2 0.241662461253461 0.0170788899505389 -2.30467079336051 6.66762032408277e-09 PITA;miRanda -1.0598585629804 4.58121558377307e-08 NA NA NA hsa-miR-130b-3p NTRK2 0.687190305027546 0.000109068658562255 -2.30467079336051 6.66762032408277e-09 mirMAP -0.437506702355608 6.88196102565667e-05 NA NA NA hsa-miR-130b-5p NTRK2 0.170599813152408 0.337611586432771 -2.30467079336051 6.66762032408277e-09 mirMAP -0.366083689585264 0.000970429145511043 NA NA NA hsa-miR-140-3p NTRK2 0.546012239205329 4.84578261098777e-11 -2.30467079336051 6.66762032408277e-09 PITA -0.621765702538706 0.00841531471617843 NA NA NA hsa-miR-148a-5p NTRK2 -0.767626696027761 1.08359477494797e-06 -2.30467079336051 6.66762032408277e-09 mirMAP -0.636528653670575 2.11782031176256e-07 NA NA NA hsa-miR-151a-5p NTRK2 0.484893436232714 4.8995594459361e-06 -2.30467079336051 6.66762032408277e-09 miRNATAP -1.33321264979205 1.53079430764618e-13 NA NA NA hsa-miR-17-3p NTRK2 0.410352776142368 0.00422200500748636 -2.30467079336051 6.66762032408277e-09 miRNATAP -0.453219975208752 0.000970000263503074 NA NA NA hsa-miR-185-5p NTRK2 0.480006257154717 6.35758891340152e-07 -2.30467079336051 6.66762032408277e-09 mirMAP -0.706902324923139 0.000526512526055176 NA NA NA hsa-miR-186-5p NTRK2 -0.060103391373687 0.535288717081205 -2.30467079336051 6.66762032408277e-09 mirMAP -0.615456145703442 0.00282417325117072 NA NA NA hsa-miR-29a-5p NTRK2 -0.113555908626946 0.349622266470788 -2.30467079336051 6.66762032408277e-09 mirMAP -0.496197551946914 0.0024225319944222 NA NA NA hsa-miR-30d-5p NTRK2 0.718283816348023 2.47422074061352e-08 -2.30467079336051 6.66762032408277e-09 mirMAP -0.544492350069766 0.000308238247398339 NA NA NA hsa-miR-32-3p NTRK2 0.219109440121582 0.207217051619543 -2.30467079336051 6.66762032408277e-09 mirMAP -0.57730597526211 7.07844368508117e-07 NA NA NA hsa-miR-32-5p NTRK2 0.0806126674664016 0.548984992497432 -2.30467079336051 6.66762032408277e-09 mirMAP -0.873196576871784 1.76759042639064e-09 NA NA NA hsa-miR-361-5p NTRK2 0.233202959858696 0.00961572812255327 -2.30467079336051 6.66762032408277e-09 miRanda;mirMAP -0.961236007085446 1.26833159807763e-05 NA NA NA hsa-miR-374a-5p NTRK2 0.016390363747302 0.869784217438621 -2.30467079336051 6.66762032408277e-09 mirMAP -1.03030330133654 1.83192331027079e-07 NA NA NA hsa-miR-374b-5p NTRK2 -0.307538028546424 0.0030079617545972 -2.30467079336051 6.66762032408277e-09 mirMAP -0.718532230574976 0.000166156599301286 NA NA NA hsa-miR-421 NTRK2 0.941856334694081 6.12167297054077e-08 -2.30467079336051 6.66762032408277e-09 mirMAP -0.382342771285455 0.00064249983773967 NA NA NA hsa-miR-452-3p NTRK2 1.76909792952222 2.98172274671408e-10 -2.30467079336051 6.66762032408277e-09 mirMAP -0.190953329238523 0.00547983200575232 NA NA NA hsa-miR-452-5p NTRK2 1.91544498799508 1.81995982853313e-14 -2.30467079336051 6.66762032408277e-09 mirMAP -0.282969267239225 0.00019818202707022 NA NA NA hsa-miR-532-5p NTRK2 1.02729546510601 9.93318913966636e-16 -2.30467079336051 6.66762032408277e-09 PITA -0.875128303330166 2.62964705553226e-09 NA NA NA hsa-miR-590-5p NTRK2 -0.103355559980404 0.310027295317092 -2.30467079336051 6.66762032408277e-09 miRanda;mirMAP -0.691661076913071 0.00040270354048443 NA NA NA hsa-miR-618 NTRK2 0.13539895350352 0.517145913114103 -2.30467079336051 6.66762032408277e-09 mirMAP -0.622793706501262 8.5230387177889e-11 NA NA NA hsa-miR-193a-3p OSMR -0.117675551589896 0.309391958208578 -1.11763186663677 5.19656003992052e-05 MirTarget -0.322994473386268 0.00629480833244995 NA NA NA hsa-miR-193b-3p OSMR -0.166697207281748 0.272022543069482 -1.11763186663677 5.19656003992052e-05 MirTarget -0.288850457393352 0.00126926914750222 NA NA NA hsa-miR-362-3p OSMR 0.814007335895913 2.00108618836568e-09 -1.11763186663677 5.19656003992052e-05 miRanda -0.294955177086112 0.00271920470184721 NA NA NA hsa-miR-199b-5p PCK1 -0.454049170125872 0.254611775331658 -3.84263732288163 1.8708607706947e-16 miRanda -0.159983199334472 0.00803410864574819 NA NA NA hsa-miR-24-3p PCK2 -0.257825594021966 0.00689742600215061 -1.76016095866022 1.12079973872433e-14 miRNAWalker2_validate -0.604988297442314 4.82577220348522e-07 NA NA NA hsa-miR-330-5p PCK2 0.442231058604099 0.0053308560843808 -1.76016095866022 1.12079973872433e-14 miRanda -0.609900404599727 1.52069930487024e-18 NA NA NA hsa-miR-548v PDGFA -0.27258775900243 0.176259917595269 1.77758746228644 4.60244665196294e-16 miRNATAP -0.278647806801463 4.33621753073177e-07 NA NA NA hsa-miR-576-5p PDGFA -0.377452008285847 0.00470735657343984 1.77758746228644 4.60244665196294e-16 mirMAP -0.349423515951374 2.28885449982783e-05 NA NA NA hsa-miR-140-5p PDGFB -0.220106443070144 0.0140713197101242 0.990792072539177 3.84799134063219e-14 miRanda -0.224478580392745 0.00250343524846709 NA NA NA hsa-miR-146b-3p PDGFB -1.20799545896423 2.19859530890688e-09 0.990792072539177 3.84799134063219e-14 miRNAWalker2_validate -0.116559540687373 0.000253813958503156 NA NA NA hsa-miR-26b-5p PDGFB -1.10804856399744 4.14176110314282e-22 0.990792072539177 3.84799134063219e-14 miRNAWalker2_validate -0.212037229772437 0.000102211416676722 NA NA NA hsa-miR-590-3p PDGFB -0.473167498047808 1.76652934637227e-05 0.990792072539177 3.84799134063219e-14 PITA;miRanda;miRNATAP -0.219997217412303 0.0002136212610643 NA NA NA hsa-miR-330-5p PDGFC 0.442231058604099 0.0053308560843808 -1.16709365311198 9.87240279618114e-13 miRanda -0.189791877631495 0.000224957238024521 NA NA NA hsa-miR-421 PDGFC 0.941856334694081 6.12167297054077e-08 -1.16709365311198 9.87240279618114e-13 miRanda -0.204828970017518 9.80030445969004e-06 NA NA NA hsa-miR-19b-1-5p PDGFD -0.277804782598538 0.0783067794722916 -0.0269386365998936 0.892577700101127 MirTarget -0.205584831358073 0.000829574339946887 NA NA NA hsa-miR-374a-5p PDGFD 0.016390363747302 0.869784217438621 -0.0269386365998936 0.892577700101127 MirTarget;mirMAP -0.299837460978545 0.00225563529280101 NA NA NA hsa-miR-590-5p PDGFD -0.103355559980404 0.310027295317092 -0.0269386365998936 0.892577700101127 miRanda -0.576413829030147 1.16311264268507e-09 NA NA NA hsa-miR-106b-5p PDGFRA 0.648901865047099 8.24870862047885e-09 -3.44515402293711 2.35408080284508e-16 MirTarget;miRNATAP -0.667814276068908 0.000339494510953522 NA NA NA hsa-miR-20a-5p PDGFRA 0.854326758727003 5.04256669663285e-07 -3.44515402293711 2.35408080284508e-16 MirTarget;miRNATAP -0.429411714989606 0.000497563648177803 NA NA NA hsa-miR-219a-5p PDGFRA 0.17600389823009 0.322694100196276 -3.44515402293711 2.35408080284508e-16 MirTarget -0.52063936637308 1.57138286329894e-05 NA NA NA hsa-miR-34a-5p PDGFRA 1.03694231790865 5.74864019540594e-14 -3.44515402293711 2.35408080284508e-16 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -1.20419826674778 7.99910511006868e-17 22750848 Mechanistically in addition to its direct regulation of platelet-derived growth factor receptor-alpha PDGFRA promoter enrichment analysis of context likelihood of relatedness-inferred mRNA nodes established miR-34a as a novel regulator of a SMAD4 transcriptional network glioblastoma hsa-miR-362-3p PDGFRA 0.814007335895913 2.00108618836568e-09 -3.44515402293711 2.35408080284508e-16 miRanda -0.626878498986425 4.42986522053939e-05 NA NA NA hsa-miR-374a-5p PDGFRA 0.016390363747302 0.869784217438621 -3.44515402293711 2.35408080284508e-16 mirMAP -0.904441562316791 2.39983127114498e-05 NA NA NA hsa-miR-374b-5p PDGFRA -0.307538028546424 0.0030079617545972 -3.44515402293711 2.35408080284508e-16 mirMAP -0.548120366479417 0.00796437271353305 NA NA NA hsa-miR-421 PDGFRA 0.941856334694081 6.12167297054077e-08 -3.44515402293711 2.35408080284508e-16 PITA;miRanda;miRNATAP -0.45565181782464 0.000157204289652031 NA NA NA hsa-miR-616-5p PDGFRA 0.150569904638223 0.402836556304524 -3.44515402293711 2.35408080284508e-16 mirMAP -0.412847880089754 0.000492619399212517 NA NA NA hsa-miR-93-5p PDGFRA 1.39518349670953 2.84167728726712e-25 -3.44515402293711 2.35408080284508e-16 MirTarget;miRNATAP -0.842533275875607 1.07321761969733e-08 NA NA NA hsa-miR-28-5p PDGFRB -0.430964830599964 2.67367257252925e-06 0.903688818724302 2.6429876964178e-08 miRanda -0.310145998507964 0.000393892179655548 NA NA NA hsa-miR-29a-3p PDGFRB -0.856762511178136 4.65330336378459e-12 0.903688818724302 2.6429876964178e-08 miRNATAP -0.17841922996433 0.00500595280694788 NA NA NA hsa-miR-29c-3p PDGFRB -1.4367517608807 1.61767774905781e-21 0.903688818724302 2.6429876964178e-08 miRNATAP -0.139148992716859 0.00615874031219039 NA NA NA hsa-miR-486-5p PDGFRB -1.77766890600022 2.9071717929922e-14 0.903688818724302 2.6429876964178e-08 miRanda -0.142065402424595 1.73938186674638e-05 NA NA NA hsa-miR-106a-5p PDPK1 -0.464229535269236 0.009724944358047 0.309856812256619 0.0145897035970451 mirMAP -0.106603481622112 0.00191993221102484 NA NA NA hsa-miR-22-5p PDPK1 -0.630365901263172 3.32415348030414e-05 0.309856812256619 0.0145897035970451 miRNATAP -0.114858817985206 0.004539264565349 NA NA NA hsa-miR-24-3p PDPK1 -0.257825594021966 0.00689742600215061 0.309856812256619 0.0145897035970451 miRNAWalker2_validate -0.189377775001018 0.00377818335280003 NA NA NA hsa-miR-27a-3p PDPK1 -0.370807380415112 0.00875721956202713 0.309856812256619 0.0145897035970451 miRNATAP -0.12746674939881 0.00357863446842311 NA NA NA hsa-miR-324-3p PDPK1 0.260348469146494 0.0506141704203545 0.309856812256619 0.0145897035970451 mirMAP -0.133875113382166 0.00407187982303137 NA NA NA hsa-miR-326 PDPK1 -1.881935132554 1.68087316863775e-21 0.309856812256619 0.0145897035970451 mirMAP -0.115285864383535 0.000115621382458437 NA NA NA hsa-miR-330-3p PDPK1 -0.332364259424489 0.0316051913061459 0.309856812256619 0.0145897035970451 mirMAP -0.199719327448706 8.51522334736582e-07 NA NA NA hsa-miR-330-5p PDPK1 0.442231058604099 0.0053308560843808 0.309856812256619 0.0145897035970451 mirMAP -0.174352569124449 6.44046033884862e-06 NA NA NA hsa-miR-331-3p PDPK1 -0.283974346994635 0.0373785411053202 0.309856812256619 0.0145897035970451 miRNAWalker2_validate -0.14393772894958 0.0015300062995931 NA NA NA hsa-miR-342-3p PDPK1 -0.319677314984262 0.0449813878317147 0.309856812256619 0.0145897035970451 mirMAP -0.202682473172801 1.2852571763811e-07 NA NA NA hsa-miR-424-5p PDPK1 -2.62653257498508 2.38997974737991e-50 0.309856812256619 0.0145897035970451 mirMAP -0.106052465451978 0.000611771334502943 NA NA NA hsa-miR-542-3p PDPK1 -1.31351015149842 3.64642423288576e-22 0.309856812256619 0.0145897035970451 mirMAP -0.149412531526728 0.000555422280439471 NA NA NA hsa-miR-744-5p PDPK1 -0.378868377317939 0.00125071229180016 0.309856812256619 0.0145897035970451 miRNAWalker2_validate -0.143178711893691 0.0067023429085085 NA NA NA hsa-miR-766-3p PDPK1 0.199979324759329 0.257230732626744 0.309856812256619 0.0145897035970451 mirMAP -0.152946333354899 1.15508230226764e-05 NA NA NA hsa-miR-1307-3p PHLPP1 0.845374887432948 8.81276312435625e-11 -0.739355893829483 5.14182264498052e-09 miRNAWalker2_validate -0.211080363137469 6.76136594543736e-06 NA NA NA hsa-miR-141-3p PHLPP1 -0.354316381352484 0.256996894288799 -0.739355893829483 5.14182264498052e-09 MirTarget;TargetScan;miRNATAP -0.122856168863493 5.12037878302015e-10 24945731 MicroRNA 141 promotes the proliferation of non small cell lung cancer cells by regulating expression of PHLPP1 and PHLPP2; Together the results of this study suggest that miR-141 and its targets PHLPP1 and PHLPP2 play critical roles in NSCLC tumorigenesis and provide potential therapeutic targets for NSCLC treatment tumorigenesis lung squamous cell cancer hsa-miR-330-5p PHLPP1 0.442231058604099 0.0053308560843808 -0.739355893829483 5.14182264498052e-09 miRanda -0.300864576951079 3.68388792878286e-15 NA NA NA hsa-miR-331-3p PHLPP1 -0.283974346994635 0.0373785411053202 -0.739355893829483 5.14182264498052e-09 miRNAWalker2_validate;MirTarget;miRNATAP -0.205011132460732 7.59434274593235e-06 NA NA NA hsa-miR-338-5p PHLPP1 -0.221898432884726 0.252390988727086 -0.739355893829483 5.14182264498052e-09 MirTarget;miRNATAP -0.111162166608847 0.000625762487183056 NA NA NA hsa-miR-484 PHLPP1 0.0857240033709656 0.453982056282229 -0.739355893829483 5.14182264498052e-09 miRNAWalker2_validate -0.27375950242864 5.61840179078663e-07 NA NA NA hsa-miR-877-5p PHLPP1 1.35669625926821 1.43008125755656e-11 -0.739355893829483 5.14182264498052e-09 miRNAWalker2_validate -0.211329845070115 1.17785359620054e-11 NA NA NA hsa-miR-106b-5p PHLPP2 0.648901865047099 8.24870862047885e-09 -0.0462325263383927 0.691559726183747 miRNATAP -0.183881939413983 0.000206398404162918 NA NA NA hsa-miR-17-5p PHLPP2 0.698861147045461 2.43854106455398e-05 -0.0462325263383927 0.691559726183747 TargetScan;miRNATAP -0.279788377056125 1.0104193404684e-17 NA NA NA hsa-miR-18a-5p PHLPP2 0.918028187958706 1.90229510425322e-05 -0.0462325263383927 0.691559726183747 miRNATAP -0.192695188596685 2.90925927539319e-14 NA NA NA hsa-miR-19a-3p PHLPP2 1.01712596153672 2.57753201450625e-07 -0.0462325263383927 0.691559726183747 mirMAP -0.177477386019693 1.52050514663494e-10 NA NA NA hsa-miR-19b-3p PHLPP2 0.601279551368824 0.00016685097085251 -0.0462325263383927 0.691559726183747 mirMAP -0.233031999669343 1.39406664489008e-11 NA NA NA hsa-miR-20a-5p PHLPP2 0.854326758727003 5.04256669663285e-07 -0.0462325263383927 0.691559726183747 miRNATAP -0.267319009474098 3.22360794507594e-17 NA NA NA hsa-miR-27b-3p PHLPP2 -0.823491817819754 9.20935302746823e-14 -0.0462325263383927 0.691559726183747 MirTarget;miRNATAP -0.140894596676105 0.00484377792767415 NA NA NA hsa-miR-32-3p PHLPP2 0.219109440121582 0.207217051619543 -0.0462325263383927 0.691559726183747 mirMAP -0.12351928056618 0.000236691821750698 26276160 MiR 32 contributed to cell proliferation of human breast cancer cells by suppressing of PHLPP2 expression; Mechanically data from luciferase reporter assays revealed that miR-32 directly targeted to the 3'-untranslated region 3'-UTR of PHLPP2 breast cancer hsa-miR-32-5p PHLPP2 0.0806126674664016 0.548984992497432 -0.0462325263383927 0.691559726183747 miRNAWalker2_validate;MirTarget;miRNATAP -0.205281504105074 9.65784302542337e-07 26276160 MiR 32 contributed to cell proliferation of human breast cancer cells by suppressing of PHLPP2 expression; Mechanically data from luciferase reporter assays revealed that miR-32 directly targeted to the 3'-untranslated region 3'-UTR of PHLPP2 breast cancer hsa-miR-335-3p PHLPP2 -0.275199247483111 0.106629165248783 -0.0462325263383927 0.691559726183747 MirTarget -0.106904063737774 0.00127303125215008 NA NA NA hsa-miR-421 PHLPP2 0.941856334694081 6.12167297054077e-08 -0.0462325263383927 0.691559726183747 miRNAWalker2_validate;miRanda -0.111043075594777 0.000544821397702489 NA NA NA hsa-miR-425-5p PHLPP2 0.585974239943864 2.23063410931402e-05 -0.0462325263383927 0.691559726183747 mirMAP -0.131113269030999 0.00126745994545728 NA NA NA hsa-miR-769-5p PHLPP2 0.219661452072771 0.0333425465750576 -0.0462325263383927 0.691559726183747 miRNAWalker2_validate -0.175162278424177 0.00151449020900691 NA NA NA hsa-miR-92a-3p PHLPP2 0.212391012518195 0.134292268089997 -0.0462325263383927 0.691559726183747 MirTarget;miRNATAP -0.326207293313479 2.74259654079791e-17 NA NA NA hsa-miR-93-5p PHLPP2 1.39518349670953 2.84167728726712e-25 -0.0462325263383927 0.691559726183747 miRNAWalker2_validate;miRNATAP -0.137804954278291 0.000513935947119601 NA NA NA hsa-miR-106b-5p PIK3AP1 0.648901865047099 8.24870862047885e-09 -0.988270592083558 1.59508939805938e-10 miRNAWalker2_validate -0.343286223185954 2.86315502534195e-07 NA NA NA hsa-miR-25-3p PIK3AP1 0.633062563556379 3.68686354834328e-10 -0.988270592083558 1.59508939805938e-10 MirTarget -0.287436970377828 0.000126615285462144 NA NA NA hsa-miR-32-5p PIK3AP1 0.0806126674664016 0.548984992497432 -0.988270592083558 1.59508939805938e-10 MirTarget -0.150341777005975 0.00903173062756873 NA NA NA hsa-miR-363-3p PIK3AP1 -0.173737427255147 0.375186188765181 -0.988270592083558 1.59508939805938e-10 MirTarget -0.142158464514692 0.000289165630248638 NA NA NA hsa-miR-421 PIK3AP1 0.941856334694081 6.12167297054077e-08 -0.988270592083558 1.59508939805938e-10 miRanda -0.154667991446389 0.000396585094121431 NA NA NA hsa-miR-501-5p PIK3AP1 1.15231083477882 2.98906150004088e-10 -0.988270592083558 1.59508939805938e-10 miRNATAP -0.108525096962749 0.00855336203554218 NA NA NA hsa-miR-17-5p PIK3CA 0.698861147045461 2.43854106455398e-05 -0.417502897789422 0.000144089141837096 miRNAWalker2_validate -0.140034439363479 1.22830047865626e-05 NA NA NA hsa-miR-339-5p PIK3CA 0.280589919878422 0.0355660282647215 -0.417502897789422 0.000144089141837096 miRanda -0.161250958910826 6.10867364493006e-05 NA NA NA hsa-miR-501-5p PIK3CA 1.15231083477882 2.98906150004088e-10 -0.417502897789422 0.000144089141837096 mirMAP -0.110297544989421 0.000121810192831398 NA NA NA hsa-miR-126-5p PIK3CB -0.428561469405594 6.6035585844222e-05 0.100967159570933 0.165951003911767 mirMAP -0.182730257201379 1.71015167377407e-08 NA NA NA hsa-miR-204-5p PIK3CB -0.537962150012103 0.0330895493358849 0.100967159570933 0.165951003911767 miRNATAP -0.108408817404481 9.83805003133513e-16 NA NA NA hsa-miR-32-5p PIK3CB 0.0806126674664016 0.548984992497432 0.100967159570933 0.165951003911767 miRNATAP -0.140663620385439 6.63155819716864e-08 NA NA NA hsa-miR-1468-5p PIK3CD -1.21475558175193 2.52286949987832e-09 -0.877858532886072 2.39332269929579e-06 MirTarget -0.114605918546385 0.00956114952826845 NA NA NA hsa-miR-30b-3p PIK3CD -0.439724365151373 0.000947180503583586 -0.877858532886072 2.39332269929579e-06 MirTarget -0.241027174305288 0.000454965508091255 NA NA NA hsa-miR-30d-5p PIK3CD 0.718283816348023 2.47422074061352e-08 -0.877858532886072 2.39332269929579e-06 MirTarget;miRNATAP -0.313277633599958 7.26196135533465e-06 NA NA NA hsa-miR-30e-5p PIK3CD -0.627815042856657 3.02205690184908e-10 -0.877858532886072 2.39332269929579e-06 MirTarget -0.253648851782905 0.00528999528593308 NA NA NA hsa-miR-616-5p PIK3CD 0.150569904638223 0.402836556304524 -0.877858532886072 2.39332269929579e-06 MirTarget -0.206581353665342 5.02538338243613e-05 NA NA NA hsa-miR-103a-3p PIK3R1 0.765568928457606 3.05497476509383e-12 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.187692597616198 0.00702590214654354 NA NA NA hsa-miR-106b-5p PIK3R1 0.648901865047099 8.24870862047885e-09 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.369244291882445 4.59379549667075e-08 NA NA NA hsa-miR-1301-3p PIK3R1 1.12156714169769 4.22404125385203e-10 -0.891953424131491 1.38465728969785e-08 MirTarget -0.26088137729301 3.97606431390706e-10 NA NA NA hsa-miR-132-3p PIK3R1 0.323796239695997 0.00272093067935091 -0.891953424131491 1.38465728969785e-08 MirTarget -0.517351445825174 2.17222827385203e-13 NA NA NA hsa-miR-17-5p PIK3R1 0.698861147045461 2.43854106455398e-05 -0.891953424131491 1.38465728969785e-08 MirTarget;TargetScan;miRNATAP -0.213954808771155 3.8587060059728e-06 NA NA NA hsa-miR-185-5p PIK3R1 0.480006257154717 6.35758891340152e-07 -0.891953424131491 1.38465728969785e-08 miRNATAP -0.255181123475439 0.00153792085091181 NA NA NA hsa-miR-188-5p PIK3R1 1.11646899351496 9.56686958408598e-11 -0.891953424131491 1.38465728969785e-08 MirTarget -0.178093775716465 5.33015486626271e-05 NA NA NA hsa-miR-200c-3p PIK3R1 -0.104675615392797 0.716957669484981 -0.891953424131491 1.38465728969785e-08 mirMAP -0.105107802462856 9.06963579759966e-05 NA NA NA hsa-miR-20a-5p PIK3R1 0.854326758727003 5.04256669663285e-07 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.137431012149634 0.00237710222818209 NA NA NA hsa-miR-21-5p PIK3R1 1.51084295924322 1.54551622173259e-34 -0.891953424131491 1.38465728969785e-08 miRNAWalker2_validate;MirTarget;miRNATAP -0.626634426633867 6.01244523464713e-31 26676464 PIK3R1 targeting by miR 21 suppresses tumor cell migration and invasion by reducing PI3K/AKT signaling and reversing EMT and predicts clinical outcome of breast cancer; Next we identified the PIK3R1 as a direct target of miR-21 and showed that it was negatively regulated by miR-21; Taken together we provide novel evidence that miR-21 knockdown suppresses cell growth migration and invasion partly by inhibiting PI3K/AKT activation via direct targeting PIK3R1 and reversing EMT in breast cancer cell migration breast cancer hsa-miR-212-3p PIK3R1 -0.288359631986802 0.100392159518997 -0.891953424131491 1.38465728969785e-08 MirTarget -0.258090192317157 3.95293509038579e-09 NA NA NA hsa-miR-221-3p PIK3R1 1.12244756358612 3.48370308238012e-13 -0.891953424131491 1.38465728969785e-08 MirTarget -0.217914846238424 7.87055620620445e-06 NA NA NA hsa-miR-222-3p PIK3R1 1.08574156066353 1.76407192348131e-11 -0.891953424131491 1.38465728969785e-08 MirTarget -0.256619417997498 3.41697791848384e-08 NA NA NA hsa-miR-324-3p PIK3R1 0.260348469146494 0.0506141704203545 -0.891953424131491 1.38465728969785e-08 MirTarget;PITA;miRNATAP -0.281792149179102 1.26555231509763e-06 NA NA NA hsa-miR-330-3p PIK3R1 -0.332364259424489 0.0316051913061459 -0.891953424131491 1.38465728969785e-08 MirTarget;PITA;miRNATAP -0.212492102885122 3.44445995574159e-05 NA NA NA hsa-miR-338-5p PIK3R1 -0.221898432884726 0.252390988727086 -0.891953424131491 1.38465728969785e-08 PITA -0.143685656767977 0.000366657184559181 NA NA NA hsa-miR-409-3p PIK3R1 -0.499649792570937 0.0526751181520117 -0.891953424131491 1.38465728969785e-08 mirMAP -0.110448878014769 0.000237041711390918 NA NA NA hsa-miR-493-5p PIK3R1 -0.198321662045422 0.502870277689198 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.103739426368655 7.76271840065789e-05 NA NA NA hsa-miR-582-5p PIK3R1 -0.680189083316822 0.00103755166534107 -0.891953424131491 1.38465728969785e-08 mirMAP -0.138461163257927 0.00021364752999012 NA NA NA hsa-miR-589-3p PIK3R1 1.16846704028095 5.88525574424201e-11 -0.891953424131491 1.38465728969785e-08 mirMAP -0.21021578613876 1.13421126603334e-06 NA NA NA hsa-miR-590-5p PIK3R1 -0.103355559980404 0.310027295317092 -0.891953424131491 1.38465728969785e-08 MirTarget;PITA;miRanda;miRNATAP -0.299776876388425 9.9413226282672e-05 NA NA NA hsa-miR-629-3p PIK3R1 -0.315338123880353 0.119091248294946 -0.891953424131491 1.38465728969785e-08 MirTarget -0.182940394280375 3.19896248240917e-06 NA NA NA hsa-miR-93-5p PIK3R1 1.39518349670953 2.84167728726712e-25 -0.891953424131491 1.38465728969785e-08 MirTarget;miRNATAP -0.319783065556515 2.89613370212511e-09 NA NA NA hsa-miR-126-3p PIK3R2 -0.652349350198172 9.37876280344772e-09 0.902461657566753 1.95242785807378e-14 miRNAWalker2_validate;miRTarBase -0.307009584408785 1.4469316595199e-09 25240815;26384552;27578985;21249429;26191164 MiR 126 3p suppresses tumor metastasis and angiogenesis of hepatocellular carcinoma by targeting LRP6 and PIK3R2; LRP6 and PIK3R2 were identified as targets of miR-126-3p; Silencing LRP6 and PIK3R2 had similar effects of miR-126-3p restoration on metastasis and angiogenesis individually in HCC cells; Furthermore the miR-126-3p level was inversely correlated with LRP6 and PIK3R2 in HCC tissues; In addition the rescue experiments indicated that the metastasis and angiogenesis functions of miR-126-3p were mediated by LRP6 and PIK3R2;MicroRNA 126 suppresses proliferation of undifferentiated BRAFV600E and BRAFWT thyroid carcinoma through targeting PIK3R2 gene and repressing PI3K AKT proliferation survival signalling pathway; In addition miR-126 was found to act as proliferation suppressor targeting PIK3R2 gene and reducing p85β a regulatory subunit of PI3K kinase protein translation and lower AKT kinase activity;MiR 126 regulates proliferation and invasion in the bladder cancer BLS cell line by targeting the PIK3R2 mediated PI3K/Akt signaling pathway; A direct regulatory relationship between miR-126 and the PIK3R2 gene was demonstrated by luciferase reporter assays; Quantitative real-time polymerase chain reaction was used to measure miR-126 and PIK3R2 expressions; Luciferase assays showed that miR-126 significantly inhibited the PIK3R2 3' untranslated region 3'UTR luciferase reporter activity P<0.05; Overexpression of miR-126 negatively regulated the target gene PIK3R2 and further inhibited the PI3K/Akt signaling pathway thereby inhibiting proliferation migration and invasion and promoting apoptosis in BLS cells;Endothelial specific intron derived miR 126 is down regulated in human breast cancer and targets both VEGFA and PIK3R2; VEGFA and PIK3R2 were confirmed as the targets of miR-126 by luciferase reporter assay and Western blot;Restoration of miR-126 in EC109 cells induced a reduction in PIK3R2 protein levels accompanied with a substantial reduction in phosphorylated AKT levels in EC109 cells suggesting impairment in PI3K/AKT signaling pathway; The luciferase reporter assay confirmed that PIK3R2 was a direct target of miR-126; Taken together our study suggests that miR-126 functions as a potential tumor suppressor in ESCC progression via regulating PI3K/AKT signaling pathway partly by targeting PIK3R2 and targeting of miR-126 may provide a novel strategy for the diagnosis and treatment of ESCC metastasis;poor survival;;;progression liver cancer;thyroid cancer;bladder cancer;breast cancer;esophageal cancer hsa-miR-28-5p PIK3R2 -0.430964830599964 2.67367257252925e-06 0.902461657566753 1.95242785807378e-14 miRanda -0.216513318430191 0.000794318920407844 NA NA NA hsa-miR-30a-5p PIK3R2 -0.626257932887855 0.000108198196973765 0.902461657566753 1.95242785807378e-14 miRNATAP -0.144672134574065 6.66511974688791e-05 NA NA NA hsa-miR-30e-5p PIK3R2 -0.627815042856657 3.02205690184908e-10 0.902461657566753 1.95242785807378e-14 miRNATAP -0.27051427660089 3.48529317139267e-06 NA NA NA hsa-miR-3607-3p PIK3R2 -2.15518588554493 1.03390728226728e-23 0.902461657566753 1.95242785807378e-14 mirMAP;miRNATAP -0.192639313478842 2.68262287342929e-14 NA NA NA hsa-miR-19a-3p PIK3R3 1.01712596153672 2.57753201450625e-07 0.635454203069077 2.81455381573105e-08 MirTarget;miRNATAP -0.104972668125809 0.000194207587349022 NA NA NA hsa-miR-19b-3p PIK3R3 0.601279551368824 0.00016685097085251 0.635454203069077 2.81455381573105e-08 MirTarget;miRNATAP -0.156884173334155 7.42851910353984e-06 NA NA NA hsa-miR-222-5p PIK3R3 0.125716360553359 0.487423909991138 0.635454203069077 2.81455381573105e-08 mirMAP -0.124074211713995 7.25281139850942e-05 NA NA NA hsa-miR-32-5p PIK3R3 0.0806126674664016 0.548984992497432 0.635454203069077 2.81455381573105e-08 MirTarget;miRNATAP -0.115094122727672 0.00654760226880116 NA NA NA hsa-miR-331-3p PIK3R3 -0.283974346994635 0.0373785411053202 0.635454203069077 2.81455381573105e-08 miRNAWalker2_validate;PITA -0.141431444954819 0.000659359723681179 NA NA NA hsa-miR-335-3p PIK3R3 -0.275199247483111 0.106629165248783 0.635454203069077 2.81455381573105e-08 mirMAP -0.159933987443154 1.18680673169693e-06 NA NA NA hsa-miR-454-3p PIK3R3 0.668749059573955 2.42670710515113e-06 0.635454203069077 2.81455381573105e-08 mirMAP -0.155284428603942 8.0835303918136e-05 NA NA NA hsa-miR-590-5p PIK3R3 -0.103355559980404 0.310027295317092 0.635454203069077 2.81455381573105e-08 miRanda -0.243402254613955 1.30668545381547e-05 NA NA NA hsa-miR-624-5p PIK3R3 -0.639533773668123 6.79146620944254e-06 0.635454203069077 2.81455381573105e-08 MirTarget -0.124942360560089 0.00156005977445166 NA NA NA hsa-miR-92a-3p PIK3R3 0.212391012518195 0.134292268089997 0.635454203069077 2.81455381573105e-08 MirTarget;miRNATAP -0.21036606255577 1.06004328001549e-07 NA NA NA hsa-miR-107 PIK3R5 0.241662461253461 0.0170788899505389 -0.89989526759384 1.62789758196776e-07 miRanda -0.349504238477674 3.20976138937126e-05 NA NA NA hsa-miR-194-3p PIK3R6 -0.772747277805343 2.9537465170191e-05 0.969138287277783 7.3402634399105e-08 MirTarget -0.356683239090725 1.39036832820421e-14 NA NA NA hsa-miR-22-3p PIK3R6 -0.628561621952407 1.33755558015523e-09 0.969138287277783 7.3402634399105e-08 MirTarget -0.377891620422119 7.523159993852e-06 NA NA NA hsa-miR-30c-1-3p PIK3R6 -1.39373825444537 6.39912952911454e-17 0.969138287277783 7.3402634399105e-08 MirTarget -0.30322315540707 3.47973489344846e-09 NA NA NA hsa-miR-30c-2-3p PIK3R6 -1.39811819128587 3.66376924527665e-15 0.969138287277783 7.3402634399105e-08 MirTarget -0.270525021610409 1.47636922968783e-08 NA NA NA hsa-miR-26b-5p PKN1 -1.10804856399744 4.14176110314282e-22 0.84789468980162 4.67080555690581e-10 miRNAWalker2_validate -0.400714070076561 2.79045275402474e-13 NA NA NA hsa-miR-17-5p PKN2 0.698861147045461 2.43854106455398e-05 -0.25851170860319 0.00639460131841185 MirTarget;TargetScan;miRNATAP -0.126496538968171 4.10282921320701e-06 NA NA NA hsa-miR-501-5p PKN2 1.15231083477882 2.98906150004088e-10 -0.25851170860319 0.00639460131841185 mirMAP -0.117174131790719 1.7771782096099e-06 NA NA NA hsa-miR-93-5p PKN2 1.39518349670953 2.84167728726712e-25 -0.25851170860319 0.00639460131841185 MirTarget;miRNATAP -0.102275120129374 0.00160307402356309 NA NA NA hsa-miR-421 PPP2CB 0.941856334694081 6.12167297054077e-08 -0.890331286940364 2.58768297455211e-18 miRNAWalker2_validate;miRanda -0.107091673318932 0.000264470518633695 NA NA NA hsa-miR-425-5p PPP2CB 0.585974239943864 2.23063410931402e-05 -0.890331286940364 2.58768297455211e-18 miRNAWalker2_validate -0.210079108287547 1.06195847865028e-08 NA NA NA hsa-miR-940 PPP2CB 0.453020092551484 0.0177054921651997 -0.890331286940364 2.58768297455211e-18 MirTarget;PITA -0.108413768942713 9.39010367602326e-05 NA NA NA hsa-miR-16-5p PPP2R1A -0.398577235179603 0.000101598495180191 0.434617655824211 2.19736728815959e-07 miRNAWalker2_validate -0.16156782548915 5.72423585631049e-05 NA NA NA hsa-miR-505-5p PPP2R1A -0.768282569967001 1.14662846595407e-05 0.434617655824211 2.19736728815959e-07 miRNAWalker2_validate -0.101132276982143 1.32519884220096e-05 NA NA NA hsa-miR-15b-5p PPP2R1B 0.230566941413564 0.0824765001857479 -0.366373279268895 0.0120240053013953 MirTarget -0.166547119923006 0.0019576167364681 NA NA NA hsa-miR-30b-5p PPP2R1B -0.54056186708617 2.44688760798341e-05 -0.366373279268895 0.0120240053013953 miRNATAP -0.174282215186143 0.00161533222972509 NA NA NA hsa-miR-30d-3p PPP2R1B -0.120562286038759 0.329549534782125 -0.366373279268895 0.0120240053013953 mirMAP -0.172420555072298 0.00291249897697552 NA NA NA hsa-miR-744-3p PPP2R1B -0.866026958622846 2.43034431433797e-08 -0.366373279268895 0.0120240053013953 MirTarget -0.151341218126979 0.000790301774844116 NA NA NA hsa-miR-106b-5p PPP2R2A 0.648901865047099 8.24870862047885e-09 -0.893386744728836 1.42691109729952e-19 MirTarget;miRNATAP -0.200118143024595 4.95341178091498e-06 NA NA NA hsa-miR-140-3p PPP2R2A 0.546012239205329 4.84578261098777e-11 -0.893386744728836 1.42691109729952e-19 PITA -0.164635101330579 0.0061294672647094 NA NA NA hsa-miR-17-5p PPP2R2A 0.698861147045461 2.43854106455398e-05 -0.893386744728836 1.42691109729952e-19 MirTarget;TargetScan;miRNATAP -0.118338916789628 7.95994648130442e-05 NA NA NA hsa-miR-20a-5p PPP2R2A 0.854326758727003 5.04256669663285e-07 -0.893386744728836 1.42691109729952e-19 MirTarget;miRNATAP -0.124111638240801 1.90528354769801e-05 NA NA NA hsa-miR-425-5p PPP2R2A 0.585974239943864 2.23063410931402e-05 -0.893386744728836 1.42691109729952e-19 MirTarget;miRNATAP -0.174560086210021 1.11318996260443e-06 NA NA NA hsa-miR-92a-3p PPP2R2A 0.212391012518195 0.134292268089997 -0.893386744728836 1.42691109729952e-19 miRNAWalker2_validate -0.102694235878115 0.00387764819688652 NA NA NA hsa-miR-93-5p PPP2R2A 1.39518349670953 2.84167728726712e-25 -0.893386744728836 1.42691109729952e-19 MirTarget;miRNATAP -0.188170128446582 6.86080187206839e-08 NA NA NA hsa-miR-126-5p PPP2R2B -0.428561469405594 6.6035585844222e-05 -0.319661735361422 0.283871715863449 MirTarget -0.411398178777023 0.00235203877837632 NA NA NA hsa-miR-192-5p PPP2R2C -0.50188499512787 0.00344713341196035 2.04406049288738 0.000156223168786157 mirMAP -0.907229698394586 2.29871744989232e-09 NA NA NA hsa-miR-194-5p PPP2R2C -0.292530160992458 0.0996141384876663 2.04406049288738 0.000156223168786157 miRNATAP -0.955546890731209 7.00101635982174e-11 NA NA NA hsa-miR-140-3p PPP2R2D 0.546012239205329 4.84578261098777e-11 -0.387206987580826 2.38322021549683e-07 mirMAP -0.144421486678596 0.00101270301665515 NA NA NA hsa-miR-139-5p PPP2R3A -2.11299531796557 8.16407076415038e-28 -0.234918525572345 0.207468150002507 miRanda -0.148444954911434 0.000695461265473248 NA NA NA hsa-miR-193b-5p PPP2R3A -0.748481053559202 3.37008423124215e-06 -0.234918525572345 0.207468150002507 MirTarget -0.166212404900123 0.00288303881465964 NA NA NA hsa-miR-34a-5p PPP2R3A 1.03694231790865 5.74864019540594e-14 -0.234918525572345 0.207468150002507 MirTarget;miRNATAP -0.266106623157513 2.83078545581018e-05 NA NA NA hsa-miR-618 PPP2R3A 0.13539895350352 0.517145913114103 -0.234918525572345 0.207468150002507 mirMAP -0.186467132486573 3.10178620499447e-05 NA NA NA hsa-miR-192-5p PPP2R3B -0.50188499512787 0.00344713341196035 1.01219188204876 5.93858615542726e-19 miRNAWalker2_validate -0.108639411524065 0.0012321422143427 NA NA NA hsa-miR-215-5p PPP2R3B -0.981544773246509 3.14452272477156e-05 1.01219188204876 5.93858615542726e-19 miRNAWalker2_validate -0.128820590683626 8.21995950564856e-08 NA NA NA hsa-miR-197-3p PPP2R5A -0.245033899783728 0.0362223947695203 0.367967554039328 0.000201292409757339 MirTarget -0.159858222331069 0.000114867261240145 NA NA NA hsa-miR-335-5p PPP2R5A -1.61347836513558 1.62757083289711e-19 0.367967554039328 0.000201292409757339 miRNAWalker2_validate -0.129221734799289 4.65625737104185e-07 NA NA NA hsa-miR-484 PPP2R5A 0.0857240033709656 0.453982056282229 0.367967554039328 0.000201292409757339 miRNAWalker2_validate -0.142511519099843 0.000814298781875799 NA NA NA hsa-miR-582-5p PPP2R5A -0.680189083316822 0.00103755166534107 0.367967554039328 0.000201292409757339 PITA;miRNATAP -0.15503416694514 1.05133889416276e-11 NA NA NA hsa-miR-590-3p PPP2R5A -0.473167498047808 1.76652934637227e-05 0.367967554039328 0.000201292409757339 miRNAWalker2_validate -0.160887720851343 0.000232546271981156 NA NA NA hsa-miR-132-3p PPP2R5C 0.323796239695997 0.00272093067935091 -0.197681049931807 0.00357159893729652 miRNATAP -0.13851439885365 5.20803505364117e-06 NA NA NA hsa-miR-1976 PPP2R5D -0.431472440543166 0.00324575010550946 0.490236074781638 1.12387325087843e-09 MirTarget;miRNATAP -0.112669007651945 3.03261127309582e-05 NA NA NA hsa-miR-146b-5p PPP2R5E 0.419719546795449 0.0457375823220155 -0.33926942209527 0.000598805950355048 miRanda -0.106437767995404 2.95231299168685e-06 NA NA NA hsa-miR-19b-3p PPP2R5E 0.601279551368824 0.00016685097085251 -0.33926942209527 0.000598805950355048 miRTarBase;MirTarget -0.105521131212065 0.000437479384635745 NA NA NA hsa-miR-30d-3p PPP2R5E -0.120562286038759 0.329549534782125 -0.33926942209527 0.000598805950355048 MirTarget -0.120884932247343 0.0020877492288168 NA NA NA hsa-miR-30d-5p PPP2R5E 0.718283816348023 2.47422074061352e-08 -0.33926942209527 0.000598805950355048 mirMAP -0.112901581761164 0.00227875690176072 NA NA NA hsa-miR-455-5p PPP2R5E -0.270546034117157 0.0581320703577731 -0.33926942209527 0.000598805950355048 miRanda -0.122888449602669 0.000278155025334759 NA NA NA hsa-miR-15a-5p PRKAA1 0.349521211668244 0.000770735998804736 -0.60864021207068 5.26267892833978e-12 MirTarget -0.127934215917556 0.00259652352236475 NA NA NA hsa-let-7b-3p PRKAA2 -1.21979213547786 5.84575581584603e-17 0.910177606708893 0.0133266185680845 mirMAP -0.385703556647416 0.00114946510675726 NA NA NA hsa-let-7b-5p PRKAA2 -0.959013717270803 5.95127281258643e-11 0.910177606708893 0.0133266185680845 miRNAWalker2_validate;MirTarget -0.399590032732691 0.000857606952583238 NA NA NA hsa-let-7c-5p PRKAA2 -1.70744661345097 2.87934212005942e-20 0.910177606708893 0.0133266185680845 MirTarget -0.323478962357034 0.000457014156060023 NA NA NA hsa-miR-125b-5p PRKAA2 -1.35767721102906 1.16564617395164e-18 0.910177606708893 0.0133266185680845 miRNATAP -0.468131740207186 2.58447444857568e-05 NA NA NA hsa-miR-130a-3p PRKAA2 -1.53229181736223 2.91460363624872e-19 0.910177606708893 0.0133266185680845 MirTarget -0.261650172386151 0.00937218581418371 NA NA NA hsa-miR-130b-3p PRKAA2 0.687190305027546 0.000109068658562255 0.910177606708893 0.0133266185680845 MirTarget -0.39214943725483 8.93727722972995e-05 NA NA NA hsa-miR-139-5p PRKAA2 -2.11299531796557 8.16407076415038e-28 0.910177606708893 0.0133266185680845 mirMAP -0.243840459298225 0.00500077362975578 NA NA NA hsa-miR-144-3p PRKAA2 -2.97536914478815 6.00546047309683e-28 0.910177606708893 0.0133266185680845 mirMAP -0.251212728741441 4.29127796324217e-05 NA NA NA hsa-miR-16-2-3p PRKAA2 -0.0336420826463706 0.805158952566973 0.910177606708893 0.0133266185680845 mirMAP -0.457570769127741 0.000536483503983079 NA NA NA hsa-miR-195-3p PRKAA2 -1.09375690196245 4.70092324819807e-09 0.910177606708893 0.0133266185680845 mirMAP -0.528536254111941 2.17986726272341e-08 NA NA NA hsa-miR-203a-3p PRKAA2 -1.34362479226226 8.69374456831293e-05 0.910177606708893 0.0133266185680845 MirTarget -0.179382015471783 0.000540688920660811 NA NA NA hsa-miR-217 PRKAA2 1.05797297540381 0.0401026692682422 0.910177606708893 0.0133266185680845 mirMAP -0.174781811555677 3.64856315207684e-07 NA NA NA hsa-miR-22-5p PRKAA2 -0.630365901263172 3.32415348030414e-05 0.910177606708893 0.0133266185680845 mirMAP -0.407034919675641 0.000519823954254811 NA NA NA hsa-miR-26a-5p PRKAA2 -0.963792031426845 1.83360999387345e-22 0.910177606708893 0.0133266185680845 mirMAP -0.782511241662933 5.79168652076068e-06 NA NA NA hsa-miR-30e-3p PRKAA2 -1.20845647373031 3.10263633528203e-25 0.910177606708893 0.0133266185680845 MirTarget -0.527801395071765 0.000287963362506242 NA NA NA hsa-miR-628-3p PRKAA2 -0.958478775877672 6.18965095549213e-08 0.910177606708893 0.0133266185680845 mirMAP -0.30159363325766 0.00298084973196401 NA NA NA hsa-miR-142-5p PRKCA -1.44575553250033 1.18797044680592e-13 0.662827897377551 1.5305893364122e-08 mirMAP -0.253211662252116 4.3295868991415e-20 NA NA NA hsa-miR-146a-5p PRKCA -0.742958238440575 0.000765983177482633 0.662827897377551 1.5305893364122e-08 mirMAP -0.137245496572864 1.03630471721186e-07 NA NA NA hsa-miR-150-5p PRKCA -1.14624309254329 1.51365525978726e-06 0.662827897377551 1.5305893364122e-08 mirMAP -0.222341791889769 1.93250353571038e-22 NA NA NA hsa-miR-181a-5p PRKCA 0.250113435902954 0.0551933249319293 0.662827897377551 1.5305893364122e-08 mirMAP -0.254408568412877 7.16377375327665e-09 NA NA NA hsa-miR-181b-5p PRKCA 0.488256489401108 0.00105321129419701 0.662827897377551 1.5305893364122e-08 mirMAP -0.205095445188407 8.90870928470555e-08 NA NA NA hsa-miR-181c-5p PRKCA -0.00639394152050299 0.969127212255928 0.662827897377551 1.5305893364122e-08 mirMAP -0.183322604105065 1.38074332954132e-07 NA NA NA hsa-miR-1976 PRKCA -0.431472440543166 0.00324575010550946 0.662827897377551 1.5305893364122e-08 miRNATAP -0.183056349310667 3.0318716310551e-06 NA NA NA hsa-miR-218-5p PRKCA -0.500091158894849 0.0398595700240766 0.662827897377551 1.5305893364122e-08 mirMAP -0.148170646737725 2.1349888993316e-10 NA NA NA hsa-miR-22-5p PRKCA -0.630365901263172 3.32415348030414e-05 0.662827897377551 1.5305893364122e-08 mirMAP -0.251052842185981 1.31444858545747e-11 NA NA NA hsa-miR-2355-3p PRKCA -0.471227472721651 0.00718598113738346 0.662827897377551 1.5305893364122e-08 mirMAP -0.157103097222143 1.75080235522893e-06 NA NA NA hsa-miR-24-2-5p PRKCA -0.570999109789414 0.000447358572601571 0.662827897377551 1.5305893364122e-08 miRNAWalker2_validate -0.215395057998791 6.57823794606976e-10 22911661 Preferential star strand biogenesis of pre miR 24 2 targets PKC alpha and suppresses cell survival in MCF 7 breast cancer cells poor survival breast cancer hsa-miR-29b-2-5p PRKCA -0.223258653034772 0.084306146519938 0.662827897377551 1.5305893364122e-08 mirMAP -0.18197054047472 4.89908312952123e-05 NA NA NA hsa-miR-330-3p PRKCA -0.332364259424489 0.0316051913061459 0.662827897377551 1.5305893364122e-08 mirMAP -0.210648698518416 2.68188227678048e-08 NA NA NA hsa-miR-381-3p PRKCA -1.11307533852141 4.70760087251745e-06 0.662827897377551 1.5305893364122e-08 mirMAP -0.100091563011784 1.9205952275757e-05 NA NA NA hsa-miR-450b-5p PRKCA -1.34333360369432 2.68386079790462e-17 0.662827897377551 1.5305893364122e-08 PITA;mirMAP;miRNATAP -0.224507062259025 6.89309401647265e-11 NA NA NA hsa-miR-495-3p PRKCA -0.751193061269953 0.00388779118736635 0.662827897377551 1.5305893364122e-08 mirMAP -0.103006977344404 3.01377161842223e-06 NA NA NA hsa-miR-505-3p PRKCA -1.20027021591555 1.27927500175937e-13 0.662827897377551 1.5305893364122e-08 miRNAWalker2_validate -0.100809044268482 0.00372474801967268 NA NA NA hsa-miR-654-3p PRKCA -1.72200544726291 6.91948971554614e-10 0.662827897377551 1.5305893364122e-08 mirMAP -0.104555302741979 1.91014801150244e-07 NA NA NA hsa-let-7e-5p PRLR 0.0350298755544731 0.811068614727804 -0.35423198863653 0.0761158267261502 miRNATAP -0.273328229298226 3.82477856037307e-05 NA NA NA hsa-let-7i-5p PRLR -0.14267936165213 0.154141689875847 -0.35423198863653 0.0761158267261502 miRNATAP -0.433060428623212 9.11939198432789e-06 NA NA NA hsa-miR-143-5p PRLR -0.42696209881145 0.00646356677356831 -0.35423198863653 0.0761158267261502 mirMAP -0.250004589770104 5.13344426170688e-05 NA NA NA hsa-miR-181c-5p PRLR -0.00639394152050299 0.969127212255928 -0.35423198863653 0.0761158267261502 miRNATAP -0.356818883265105 8.04944459289478e-10 NA NA NA hsa-miR-218-5p PRLR -0.500091158894849 0.0398595700240766 -0.35423198863653 0.0761158267261502 miRNATAP -0.192599605107286 1.11838403979325e-06 NA NA NA hsa-miR-22-5p PRLR -0.630365901263172 3.32415348030414e-05 -0.35423198863653 0.0761158267261502 mirMAP -0.371336595273348 3.12675979858811e-09 NA NA NA hsa-miR-30a-3p PRLR -1.52720594401486 1.3830066075708e-17 -0.35423198863653 0.0761158267261502 mirMAP -0.146405189240048 0.00508502911838107 NA NA NA hsa-miR-30a-5p PRLR -0.626257932887855 0.000108198196973765 -0.35423198863653 0.0761158267261502 miRNATAP -0.188173220453369 0.00164448573282991 NA NA NA hsa-miR-330-3p PRLR -0.332364259424489 0.0316051913061459 -0.35423198863653 0.0761158267261502 mirMAP;miRNATAP -0.227712677594328 0.000397116147011915 NA NA NA hsa-miR-106b-5p PTEN 0.648901865047099 8.24870862047885e-09 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;miRNATAP -0.153848683464067 0.000300263137234544 24842611;26238857;26722252 MicroRNA 106b in cancer associated fibroblasts from gastric cancer promotes cell migration and invasion by targeting PTEN;We further identified PTEN and p21 as novel direct targets of miR-106b by using target prediction algorithms and a luciferase assay; Overexpression of miR-106b reduced the expression of PTEN and p21 and increased the expression of p-AKT which is a downstream of PTEN; Restoring the expression of PTEN or p21 in stably miR-106b-overexpressed cells could rescue the effect of miR-106b on cell radioresistance; These observations illustrated that miR-106b could induce cell radioresistance by directly targeting PTEN and p21 this process was accompanied by tumour-initiating cell capacity enhancement which is universally confirmed to be associated with radioresistance;Cantharidin modulates the E2F1/MCM7 miR 106b 93/p21 PTEN signaling axis in MCF 7 breast cancer cells cell migration;drug resistance; gastric cancer;colorectal cancer;breast cancer hsa-miR-130b-3p PTEN 0.687190305027546 0.000109068658562255 -0.546254680094508 2.77576587245444e-08 MirTarget;miRNATAP -0.10192313870156 0.000167797598748454 26837847;25637514 The miR 130 family promotes cell migration and invasion in bladder cancer through FAK and Akt phosphorylation by regulating PTEN; In clinical bladder cancer specimens downregulation of PTEN was found to be closely correlated with miR-130 family expression levels;MiR 130b plays an oncogenic role by repressing PTEN expression in esophageal squamous cell carcinoma cells; We confirmed that miR-130b interacted with the 3'-untranslated region of PTEN and that an increase in the expression level of miR-130b negatively affected the protein level of PTEN; However the dysregulation of miR-130b had no obvious impact on PTEN mRNA; As Akt is a downstream effector of PTEN we explored if miR-130b affected Akt expression and found that miR-130b indirectly regulated the level of phosphorylated Akt while total Akt protein remained unchanged; The results indicate that miR-130b plays an oncogenic role in ESCC cells by repressing PTEN expression and Akt phosphorylation which would be helpful in developing miRNA-based treatments for ESCC cell migration; bladder cancer;esophageal cancer hsa-miR-132-3p PTEN 0.323796239695997 0.00272093067935091 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.25299424939486 1.34753285647319e-08 NA NA NA hsa-miR-148b-3p PTEN 0.266862251671911 0.00185271302045935 -0.546254680094508 2.77576587245444e-08 MirTarget;miRNATAP -0.178788666527829 0.00179323404620052 NA NA NA hsa-miR-15b-3p PTEN 0.238105490431193 0.0997721500357416 -0.546254680094508 2.77576587245444e-08 mirMAP -0.176228162620837 1.2020702896027e-07 NA NA NA hsa-miR-16-2-3p PTEN -0.0336420826463706 0.805158952566973 -0.546254680094508 2.77576587245444e-08 mirMAP -0.157329566369689 9.77918616060322e-06 NA NA NA hsa-miR-181b-5p PTEN 0.488256489401108 0.00105321129419701 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;MirTarget;miRNATAP -0.114085044640472 0.000438213061284522 NA NA NA hsa-miR-186-5p PTEN -0.060103391373687 0.535288717081205 -0.546254680094508 2.77576587245444e-08 mirMAP;miRNATAP -0.166974882115203 0.000984017106604637 NA NA NA hsa-miR-193a-3p PTEN -0.117675551589896 0.309391958208578 -0.546254680094508 2.77576587245444e-08 PITA;miRanda -0.131417170977765 0.00187315114851493 26753960;23223432 Downregulation of microRNA 193 3p inhibits tumor proliferation migration and chemoresistance in human gastric cancer by regulating PTEN gene;Our study identifies miR-193a and PTEN as targets for AML1/ETO and provides evidence that links the epigenetic silencing of tumor suppressor genes miR-193a and PTEN to differentiation block of myeloid precursors drug resistance;differentiation gastric cancer;acute myeloid leukemia hsa-miR-21-5p PTEN 1.51084295924322 1.54551622173259e-34 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;mirMAP -0.235206406510716 3.9272579156044e-11 23684551;17681183;25973032;26311740;26387181;22267008;26384051;22322462;27644439;21471222;21468550;20113523;26905520;21806946;24154840;27611950;24780321;25027758;23036707;26559642;26731559;26847601;27350731;24331411;21820606;25909227;24930006;24293118;26289851;22547075;26666820;24324076;23201752;25963606;27188433;22958183;22956424;19730150;21842656;21104017;23894315;23548551;26236156;23951172;24460329;20092645;25563770;24659669;25647415;26230405;25543482;20048743;27725205;23466500;22922228;25058005;20223231;27220494;22678116;24763002;24221338;22120473;21408027;23174819;22832383;19212625;22978663;25799148;26741162;23226804;26787105;26864640;25603978;26975392 MicroRNA 21 suppresses PTEN and hSulf 1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways;MicroRNA 21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer; PTEN was shown to be a direct target of miR-21 and to contribute to miR-21 effects on cell invasion; Modulation of miR-21 altered focal adhesion kinase phosphorylation and expression of matrix metalloproteases 2 and 9 both downstream mediators of PTEN involved in cell migration and invasion; Aberrant expression of miR-21 can contribute to HCC growth and spread by modulating PTEN expression and PTEN-dependent pathways involved in mediating phenotypic characteristics of cancer cells such as cell growth migration and invasion;PDCD4 and PTEN expression was decreased gradually after tumor induction and negatively correlated with miR-21 expression; Our in vivo experiments further confirmed that miR-21 plays an important role in promoting the occurrence and development of HCC by regulating PDCD4 and PTEN;Exposure of HCC cells to sorafenib led to an increase in miR-21 expression a decrease in PTEN expression and sequential Akt activation;In addition treated with 5-AZA resulted in significant increases of miR-21 expression in both MCF-7 and MDA-MB-231 cells P < 0.01 with the protein level of PTEN increased in MCF-7 cell which was further involved in the downregulation of AKT;microRNA 21 promotes tumor proliferation and invasion in gastric cancer by targeting PTEN; Thus in this study we focused on the expression and significance of miR-21 in gastric cancer tissues and the role of miR-21 in the biological behaviour and the expression of PTEN in gastric cancer cells; Western blotting and the Luciferase Reporter Assay were used to evaluate the change of PTEN expression after lowered expression of miR-21 in gastric cancer cell lines; The western blot results and Luciferase Reporter Assay demonstrated that PTEN expression was remarkably increased after miR-21 inhibition P<0.05 microRNA-21 expression was upregulated in gastric carcinoma tissues and was significantly associated with the degree of differentiation of tumour tissues local invasion and lymph node metastasis;Furthermore we identified that miR-21 overexpression could promote Hela and U2OS cells proliferation by targeting phosphatase-tensin homolog PTEN the result of which can be rescued by miR-21 inhibitor;Inhibition of microRNA-21 mir‑21 induced upregulation of Spry2 and PTEN which underscores the importance of mir-21 in Spry2-associated tumorigenesis of the colon;Bmi-1 also regulates p53 and PTEN via miR-21;Here we demonstrated that miR-21 expression was up-regulated and its function was elevated in HER2+ BT474 SKBR3 and MDA-MB-453 breast cancer cells that are induced to acquire trastuzumab resistance by long-term exposure to the antibody whereas protein expression of the PTEN gene a miR-21 target was reduced; Rescuing PTEN expression with a p3XFLAG-PTEN-mut construct with deleted miR-21 targeting sequence at its 3' UTR restored the growth inhibition of trastuzumab in the resistant cells by inducing PTEN activation and AKT inhibition; In vivo administering miR-21 antisense oligonucleotides restored trastuzumab sensitivity in the resistant breast cancer xenografts by inducing PTEN expression whereas injection of miR-21 mimics conferred trastuzumab resistant in the sensitive breast tumors via PTEN silence;The expression of miR-21 and its target PTEN was determined by real-time qRT-PCR and western blotting respectively in tumor tissues as well as adjacent non-tumor mucosa; miR-21 was significantly up-regulated in tumor tissues while PTEN was expressed in lower levels compared to non-tumor tissues; A negative correlation between expression of miR-21 and PTEN was established in vivo;MicroRNA 21 inhibitor sensitizes human glioblastoma cells U251 PTEN mutant and LN229 PTEN wild type to taxol; Human glioblastoma U251 PTEN-mutant and LN229 PTEN wild-type cells were treated with taxol and the miR-21 inhibitor in a poly amidoamine PAMAM dendrimer alone or in combination; Interestingly the above data suggested that in both the PTEN mutant and the wild-type GBM cells miR-21 blockage increased the chemosensitivity to taxol; Thus the miR-21 inhibitor might interrupt the activity of EGFR pathways independently of PTEN status;The effect of ZA on miR-21 expression was quantified by qRT-PCR and the amount of PTEN protein and its targets were analyzed by Western blot;NTR1 activation stimulates expression of miR-21 and miR-155 in colonocytes via Akt and NF-κB to down-regulate PTEN and SOCS1 and promote growth of tumors in mice;Correlations between the expression levels of miR-21 PTEN and p-AKT were analyzed by real-time PCR and Western blot test in HER2-positive GC cell lines; Overexpression of miR-21 down-regulated PTEN expression increased AKT phosphorylation and did not affect HER2 expression; Inversely suppression of miR-21 increased PTEN expression and down-regulated AKT phosphorylation but still did not affect HER2 expression;MicroRNA 21 promotes TGF β1 induced epithelial mesenchymal transition in gastric cancer through up regulating PTEN expression; In GC tissues the expressions of miR-21 Akt and p-Akt were up-regulated while PTEN expression was down-regulated; These results suggest that miR-21 could promote TGF-β1-induced EMT in GC cells through up-regulating PTEN expression;Expressions of PTEN were significantly down-regulated in CRC tissues and negatively correlated with expressions of Notch-1 r2=0.5207 p<0.01 and miR-21 r2=0.6996 p<0.01; These data indicate that the crosstalk between Notch-1 and miR-21 is involved in CRC development through degradation of PTEN;In the same patients we also compared miR-21 expression with the expression of its presumed target PTEN; miR-21 expression levels were found to significantly correlate with tumour size r = 0.403 p = 0.009; Spearman's rank whereas no relation was found between miR-21 and PTEN expression levels Kruskal-Wallis test;Over-expression of miR-21 suppressed its target PTEN and disrupted acinar morphogenesis;There was overexpression of the miR-21 target genes PTEN by 67% and caspase-3 by 15% upon cotreatment;We previously reported that microRNA-21 miR-21 was strongly expressed in melanoma relative to naevi and now sought to further assess the significance of this by assessing its relationship with its putative target PTEN; Clinical melanoma samples were analysed by immunohistochemical analysis for PTEN stem-loop qRT-PCR for miR-21 and PCR for BRAF/NRAS mutation status; miR-21 expression was inversely associated with nuclear PTEN expression but not with cytoplasmic PTEN expression; These data suggest miR-21 may exert an oncogenic effect in melanoma by favouring redistribution of PTEN to the nucleus;Triptolide reduces proliferation and enhances apoptosis of human non small cell lung cancer cells through PTEN by targeting miR 21; To the best of our knowledge the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21;Quantitative real-time PCR qRT-PCR and Western blot were used to detect the expression levels of miR-21 and PTEN in HCT116 HT29 Colo32 and SW480 CRC cell lines; Also the expression levels of PTEN mRNA and its downstream proteins AKT and PI3K in HCT116 cells after downregulating miR-21 were investigated; In comparing the levels of PTEN protein and downstream AKT and PI3K in HCT116 cells after downregulation of miR-21 expression the levels of AKT and PI3K protein expression significantly decreased P < 0.05; PTEN is one of the direct target genes of miR-21;The level of miR-21 was reversely correlated with the expression of PTEN and PDCD4 and positive correlated with PI3K/Akt pathway; miR-21 is involved in acquired resistance of EGFR-TKI in NSCLC which is mediated by down-regulating PTEN and PDCD4 and activating PI3K/Akt pathway;MicroRNA 21 modulates chemosensitivity of breast cancer cells to doxorubicin by targeting PTEN; TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-21 and tumor suppressor gene PTEN protein; We showed that upregulation of miR-21 in MCF-7/ADR cells was concurrent with downregulation of PTEN protein; Overexpression of PTEN could mimic the same effects of miR-21 inhibitor in MCF-7/ADR cells and PTEN-siRNA could increase the resistance of MCF-7 cells to ADR; MiR-21 inhibitor could increase PTEN protein expression and the luciferase activity of a PTEN 3' untranslated region-based reporter construct in MCF-7/ADR cells; Dysregulation of miR-21 plays critical roles in the ADR resistance of breast cancer at least in part via targeting PTEN;MiR-21 level was inversely correlated with the levels of FOXO1 and PTEN in DLBCL cell lines; MiR-21 also down-regulated PTEN expression and consequently activated the PI3K/AKT/mTOR pathway which further decreased FOXO1 expression;In present study we determined the miR-21 levels in TNBC specimens and TNBC cell levels in vitro and then identified the role of miR-21 on tumor cell proliferation apoptosis and then identified PTEN as the possible target of the microRNA;MiR 21 suppresses the anticancer activities of curcumin by targeting PTEN gene in human non small cell lung cancer A549 cells; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA was performed to modulate the expression of microRNA-21 and PTEN under the treatment of curcumin; Moreover the protein level of PTEN a putative target of microRNA-21 was significantly elevated in curcumin-treated A549 cells as determined by Western blot analysis; Transfection of A549 cells with microRNA-21 mimic or PTEN small interfering RNA significantly P < 0.05 reversed the growth suppression and apoptosis induction by curcumin compared to corresponding controls; Our data suggest a novel molecular mechanism in which inhibition of microRNA-21 and upregulation of PTEN mediate the anticancer activities of curcumin in NSCLC cells;PTEN gene expression was performed as a known target of miR-21;Induction of miR-21 may enable cancer cells to elude DNA damage-induced apoptosis and enhance the metastatic potential of breast cancer cells through repressing expression of PTEN and PDCD4;PTEN a direct target gene of miR-21 was significantly downregulated in gemcitabine-resistant breast cancer cells and restoration of PTEN expression blocked miR-21-induced EMT and gemcitabine resistance;We also found that Rawq01 up-regulated the expression of PTEN through mir-21 inhibition and therefore inhibited the PI3K-AKT pathway;Moreover we demonstrate that oligonucleotide-mediated miR-21 silencing in U87 human GBM cells resulted in increased levels of the tumor suppressors PTEN and PDCD4 caspase 3/7 activation and decreased tumor cell proliferation;Overexpression of miR 21 promotes the proliferation and migration of cervical cancer cells via the inhibition of PTEN; The aim of this study was to examine the expression of miR-21 and PTEN in cervical cancer specimens using quantitative PCR; miR-21 was upregulated in the cervical cancer specimens negatively correlating with the PTEN mRNA level; Transfection of the miR-21 mimics was markedly promoted whereas the miR-21 inhibitor suppressed the proliferation migration and invasion of cervical cancer cells with a significant inhibition of PTEN expression; The present study showed the upregulation of miR-21 in invasive cervical cancers and confirmed the promotion of miR-21 with regard to the proliferation migration and invasion in cervical cancer cells via inhibiting the PTEN expression;The expressions of miR-21 PTEN PI3K and AKT were detected in 89 esophageal cancer samples and 58 adjacent normal tissues respectively; MiR-21 PI3K and AKT have higher expressions but PTEN has lower expression in esophageal cancer tissues compared with adjacent normal tissues; Further PTEN was a target gene of miR-21;The expression level of miR-21 and PTEN messenger RNA were measured by quantitative real-time reverse transcription polymerase chain reaction or reverse transcription polymerase chain reaction; miR-21 was overexpressed and PTEN was suppressed in established radioresistant TE-R60 cells compared with the parent cells 1.3-fold and 70.83%; The inhibition of miR-21 significantly increased the cells' radiosensitivity P < 0.05 and the PTEN protein expression 2.3-fold in TE-1 cells; Knockdown of PTEN in anti-miR-21 TE-1 cells could abrogate the miR-21 inhibition-induced radiosensitization P < 0.05; Inhibition of miR-21 increased radiosensitivity of esophageal cancer TE-1 cells and this effect was possibly through the activation of PTEN;MicroRNA 21 miR 21 expression promotes growth metastasis and chemo or radioresistance in non small cell lung cancer cells by targeting PTEN; Taken together these results provide evidence to show the promotion role of miR-21 in NSCLC development through modulation of the PTEN signaling pathway;Protein levels of tumor suppressor targets of the miRNAs were increased by antisense to miR-21 PTEN and RECK and miR-221 p27;The expression of miR-21 PTEN and PDCD4 were determined by Real-time PCR; Glossy ganoderma spore oil down-regulated the expression of miR-21 and up-regulated the expression of PTEN and PDCD4 significantly;Role of microRNA 21 and effect on PTEN in Kazakh's esophageal squamous cell carcinoma; To evaluate the role of miR-21 and PTEN cell proliferations were analyzed with miR-21 mimics or their inhibitor-transfected cells; In Eca109 when transfected with miR-21 mimics accumulation of miR-21 was obviously increased and expression of PTEN protein was decreased to be approximately 40% which resulted in the promotion of cell proliferation; However when transfected with miR-21 inhibitor expression of miR-21 was declined and PTEN protein was overexpressed to be approximately 79% which resulted in the suppression of cell proliferation; Furthermore there was a significantly inverse correlation between miR-21 expression and PTEN protein levels p < 0.05; The author concluded that MiR-21 was overexpressed in vitro and ESCC and promoted the cell proliferation might target PTEN at post-transcriptional level and regulated the cancer invasion in Kazakh's ESCC;Difluorinated curcumin CDF restores PTEN expression in colon cancer cells by down regulating miR 21; Indeed our current data demonstrate a marked downregulation of PTEN in SCID mice xenografts of miR-21 over-expressing colon cancer HCT116 cells; Colonospheres that are highly enriched in cancer stem/stem like cells reveal increased miR-21 expression and decreased PTEN; Difluorinated curcumin CDF a novel analog of the dietary ingredient curcumin which has been shown to inhibit the growth of 5-Flurouracil + Oxaliplatin resistant colon cancer cells downregulated miR-21 in chemo-resistant colon cancer HCT116 and HT-29 cells and restored PTEN levels with subsequent reduction in Akt phosphorylation;Moreover knockdown of miR-21 increased PDCD4 and PTEN expression at the protein level but not at the mRNA level;Mechanistic evidence showed that down-regulation of miR-21 increased the expression of its target molecule PTEN in HCT116 cells;The results showed that knockdown of miR-21 by antagomir-21 decreased cell proliferation and induced apoptosis via targeting PTEN both in 4T1 cells and HUVECs;Finally invasion and metastasis assays were performed and alteration in mir-21 PTEN AKT and pAKT level was evaluated in these cells; Enhanced invasion and metastasis increased miR-21 expression decreased PTEN elevated pAKT level were demonstrated in gemcitabine-resistant HPAC and PANC-1 cells;In the present study we investigated the role of miR-21 and its potential as a therapeutic target in two prostate cancer cell lines characterized by different miR-21 expression levels and PTEN gene status;Expressions of microRNA-21 miR-21 PTEN MMP9 and p47 were detected by qPCR;The protein levels of miR-21 targets PTEN and PDCD4 were estimated; In the control experiment miR-21 mimic significantly inhibited the expression of PTEN and PDCD4 proteins in the two gastric cell lines leading to an increase in cell invasion and migration; miR-21 is overexpressed in gastric cancer and its aberrant expression may have important role in gastric cancer growth and dissemination by modulating the expression of the tumor suppressors PTEN and PDCD4 as well as by modulating the pathways involved in mediating cell growth migration invasion and apoptosis;MiR-21 upregulation contributes to PTEN downregulation which is beneficial for the activation of PI3K/AKT signaling;microRNA 21 Regulates Cell Proliferation and Migration and Cross Talk with PTEN and p53 in Bladder Cancer; MicroRNA-21 regulates proliferation and migration of bladder cancer cells and cross talk with PTEN and p53 in bladder cancer;Moreover knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level;Downregulation of miR 21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status; To explore whether miR-21 can serve as a therapeutic target for glioblastoma we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 PTEN mutant and LN229 PTEN wild-type GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo; Taken together our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status;The results showed that upon exposure to mUA miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated; In conclusion our data suggest that mUA can suppress cell viability in DU145 cells through modulating miR-21 and its downstream series-wound targets including PTEN Akt and Wnt/β-catenin signaling;miR 21 confers cisplatin resistance in gastric cancer cells by regulating PTEN; In addition miR-21 induced cell survival and cisplatin resistance through downregulating the expression of phosphatase and tension homolog deleted on chromosome 10 PTEN and activation of Akt pathway;Finally we demonstrate that modulation of tumor suppressors PTEN and p53 in U87 cells does not affect the decrease in miR-21 levels associated with PDGF-B overexpression;This study aimed to investigate whether NSCLC miR-21 mediated resistance to TKIs also results from Pten targeting; Here we show miR-21 promotes cancer by negatively regulating Pten expression in human NSCLC tissues: high miR-21 expression levels were associated with shorter DFS in 47 NSCLC patients; high miR-21/low Pten expression levels indicated a poor TKI clinical response and shorter overall survival in another 46 NSCLC patients undergoing TKI treatment; In vitro assays showed that miR-21 was up-regulated concomitantly to down-regulation of Pten in pc-9/GR cells in comparison with pc-9 cells; Moreover over-expression of miR-21 significantly decreased gefitinib sensitivity by down-regulating Pten expression and activating Akt and ERK pathways in pc-9 cells while miR-21 knockdown dramatically restored gefitinib sensitivity of pc-9/GR cells by up-regulation of Pten expression and inactivation of AKT and ERK pathways in vivo and in vitro;MicroRNA 21 miR 21 represses tumor suppressor PTEN and promotes growth and invasion in non small cell lung cancer NSCLC; We identified the role of miR-21 in non-small cell lung cancer NSCLC and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation; Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot respectively; Tumor tissues showed an inverse correlation between miR-21 and PTEN protein; miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines; miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC;MiR-21 overexpression decreases PTEN increases p-Akt and subsequently increases HIF-1α expression while miR-21 inhibition results in increased PTEN decreased p-Akt and then decreased HIF-1α;Furthermore miR-21-induced upregulation of CSF-1 mRNA and its transcription were prevented by expression of PTEN mRNA lacking 3'-untranslated region UTR and miR-21 recognition sequence; Our results reveal a novel mechanism for the therapeutic function of fish oil diet that blocks miR-21 thereby increasing PTEN levels to prevent expression of CSF-1 in breast cancer;This study was aimed to investigate the expression of microRNA-21 and its correlation with PTEN in diffuse large B cell lymphoma DLBCL paraffin-embedded tissues and evaluate its potential relevance with clinical characteristics; In patients with DLBCL the expression level of miR-21 was negatively correlated with the level of PTEN protein; These findings suggest that PTEN is possibly one of the targets of miR-21 in DLBCL;Down regulation of PTEN expression modulated by dysregulated miR 21 contributes to the progression of esophageal cancer; We demonstrated that knockdown of miR-21 significantly increased expression of PTEN protein; Our findings suggest that miR-21 could be a potential oncomiR probably by regulation of PTEN and a novel prognostic factor for ESCC patients;The levels of mir-21 did not associate with the expression of PTEN an important tumour suppressor in CRC and one of many putative targets of miR-21 but interestingly was associated with stage of disease in the PTEN expressing tumours;Anti tumor activity of a novel compound CDF is mediated by regulating miR 21 miR 200 and PTEN in pancreatic cancer; In a xenograft mouse model of human PC CDF treatment significantly inhibited tumor growth which was associated with decreased NF-κB DNA binding activity COX-2 and miR-21 expression and increased PTEN and miR-200 expression in tumor remnants;The PTEN protein levels in CRC tissues and cells had an inverse correlation with miR-21 expression; MiR-21 targets PTEN at the post-transcriptional level and regulates cell proliferation and invasion in CRC;After miR-21 was transfected in MCF-7 cells PTEN protein level was measured by Western blot; Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt resulting in accumulation of Bad p21/WAF1/CIP1 and p27/KIP1;To further determine the potential involvement of miR-21 in breast cancer we have evaluated the expression level of miR-21 by stem-loop real-time RT-PCR based on SYBR-Green I in human invasive ductal carcinoma of the breast and we have correlated the results with clinicopathologic features and PTEN protein expression; The expression levels of miR-21 were correlated with PTEN and commonly used clinicopathologic features of breast cancer; Expression of miR-21 was negatively correlated with expression of PTEN P=0.013; These findings suggest that PTEN is possibly one of the targets of miR-21 in breast cancer and high expression of mir-21 indicates a more aggressive phenotype;These findings demonstrate a novel role of AR in the regulation of miR-21 and its target PTEN in growth factor-induced colon cancer cell growth;Whether miR-21 regulated PTEN expression was assessed by luciferase assay; The RNA and protein levels of PTEN were significantly decreased by exogenous miR-21 and the 3'-untranslated region of PTEN was shown to be a target of miR-21;In vitro study showed QTsome/AM-21 induced upregulation of miR-21 targets including PTEN and DDAH1 in A549 cells while increasing their sensitivity toward paclitaxel PTX;microRNA 21 overexpression contributes to cell proliferation by targeting PTEN in endometrioid endometrial cancer; We performed a qRT-PCR assay with miR-21 and PTEN in 16 paired EEC tumor tissues and adjacent non-tumor endometrium; To validate the putative binding site of miR-21 in the 3' untranslated region 3'-UTR of PTEN messenger RNA mRNA a dual-luciferase reporter assay was carried out; The upregulation of miR-21 led to a significant decrease in the PTEN protein expression level P=0.007; The downregulation of miR-21 led to a significant increase in PTEN protein P=0.002; In conclusion we demonstrated that the expression of PTEN protein but not mRNA was negatively directly regulated by miR-21 in the KLE cell line; The overexpression of miR-21 modulated EEC cell proliferation through the downregulation of PTEN;The prognostic effect of PTEN expression status in colorectal cancer development and evaluation of factors affecting it: miR 21 and promoter methylation; In this study we investigated the effect of miR-21 and promoter methylation on the PTEN expression status in CRC tissues and analyzed association of the PTEN expression status with clinicopathological features in patients with CRC; PTEN mRNA level was negatively correlated with miR-21 level r = -0.595 P < 0.001; PTEN expression was also correlated directly with the PTEN mRNA level r = 0.583 P < 0.001 and conversely with miR-21 level r = -0.632 P < 0.001; This study suggests a high frequency of miR-21 overexpression and aberrant promoter methylation in down-regulation of PTEN expression in colorectal carcinoma;MicroRNA 21 induces 5 fluorouracil resistance in human pancreatic cancer cells by regulating PTEN and PDCD4; The proresistance effects of miR-21 were attributed to the attenuated expression of tumor suppressor genes including PTEN and PDCD4;MicroRNA 21 controls hTERT via PTEN in human colorectal cancer cell proliferation; The aim of this study was to determine a role of microRNA-21 miRNA-21 in colorectal cancer CRC and to elucidate miRNA-21 regulation of hTERT by phosphatase and tensin homologue PTEN;Relevance of miR 21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells; We identified the tumor suppressor gene PTEN as a target of miR-21 and determined the mechanism of its regulation throughout reporter construct plasmids; Using this model we analyzed the expression of miR-21 and PTEN as well as functional effects such as autophagy and apoptosis induction; In SiHa cells there was an inverse correlation between miR-21 expression and PTEN mRNA level as well as PTEN protein expression in cervical cancer cells; We conclude that miR-21 post-transcriptionally down-regulates the expression of PTEN to promote cell proliferation and cervical cancer cell survival progression;cell migration;;;;metastasis;differentiation;;tumorigenesis;;drug resistance;;;;;;;;;;;;;;drug resistance;drug resistance;;;;;;drug resistance;;;;;;metastasis;drug resistance;;;;;;;;metastasis;;;;;;;progression;malignant trasformation;;drug resistance;poor survival;;drug resistance;poor survival;;;;;progression;staging;;;;;;;;;;drug resistance;;poor survival liver cancer;liver cancer;liver cancer;liver cancer;breast cancer;gastric cancer;cervical and endocervical cancer;colon cancer;gastric cancer;breast cancer;bladder cancer;glioblastoma;breast cancer;colon cancer;gastric cancer;gastric cancer;colorectal cancer;breast cancer;lung cancer;glioblastoma;melanoma;lung squamous cell cancer;colorectal cancer;lung squamous cell cancer;breast cancer;B cell lymphoma;breast cancer;lung squamous cell cancer;thyroid cancer;breast cancer;breast cancer;esophageal cancer;glioblastoma;cervical and endocervical cancer;esophageal cancer;esophageal cancer;lung squamous cell cancer;pancreatic cancer;lung cancer;esophageal cancer;colon cancer;B cell lymphoma;colon cancer;breast cancer;pancreatic cancer;prostate cancer;lung cancer;gastric cancer;breast cancer;bladder cancer;B cell lymphoma;glioblastoma;prostate cancer;gastric cancer;glioblastoma;lung squamous cell cancer;lung squamous cell cancer;cervical and endocervical cancer;breast cancer;B cell lymphoma;esophageal cancer;colorectal cancer;pancreatic cancer;colorectal cancer;breast cancer;breast cancer;colon cancer;lung squamous cell cancer;lung cancer;endometrial cancer;colorectal cancer;pancreatic cancer;colorectal cancer;cervical and endocervical cancer hsa-miR-212-3p PTEN -0.288359631986802 0.100392159518997 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.126653228047854 4.35312213024863e-06 NA NA NA hsa-miR-25-3p PTEN 0.633062563556379 3.68686354834328e-10 -0.546254680094508 2.77576587245444e-08 miRTarBase;MirTarget;miRNATAP -0.17145581255423 0.000296261160928615 NA NA NA hsa-miR-425-5p PTEN 0.585974239943864 2.23063410931402e-05 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.14409935403185 3.42816202938787e-05 25154996 An increase in miR-425 depended upon IL-1β-induced NF-kappaB activation.Repression of PTEN by miR-425 promoted gastric cancer cell proliferation gastric cancer hsa-miR-484 PTEN 0.0857240033709656 0.453982056282229 -0.546254680094508 2.77576587245444e-08 miRNATAP -0.198915392224346 2.77954860077785e-06 NA NA NA hsa-miR-589-3p PTEN 1.16846704028095 5.88525574424201e-11 -0.546254680094508 2.77576587245444e-08 MirTarget;mirMAP -0.104104404805544 0.000121296389317292 NA NA NA hsa-miR-590-5p PTEN -0.103355559980404 0.310027295317092 -0.546254680094508 2.77576587245444e-08 mirMAP -0.170198210686497 0.000404726658138002 23803188 Targetscan predicted PDCD4 and PTEN as the potential target genes of miR-590-5p and miR-590-3p which was verified by luciferase reporter system and Western blotting liver cancer hsa-miR-93-5p PTEN 1.39518349670953 2.84167728726712e-25 -0.546254680094508 2.77576587245444e-08 miRNAWalker2_validate;miRTarBase;miRNATAP -0.151328205041358 8.0568838043516e-06 25633810;26243299;22465665;26087719 MicroRNA 93 activates c Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A; We confirmed that miR-93 directly bound with the 3' untranslated regions of the tumor-suppressor genes PTEN and CDKN1A respectivelyand inhibited their expression; We concluded that miR-93 stimulated cell proliferation migration and invasion through the oncogenic c-Met/PI3K/Akt pathway and also inhibited apoptosis by directly inhibiting PTEN and CDKN1A expression in human HCC;microRNA 93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells; An miRNA miR-93 was significantly up-regulated whereas phosphatase and tensin homologue PTEN expression was significantly down-regulated in all tested OS cells when compared with hMSCs; Ectopic expression of miR-93 decreased PTEN protein levels; Taking these observations together miR-93 can be seen to play a critical role in carcinogenesis through suppression of PTEN and may serve as a therapeutic target for the treatment of OS;Furthermore we found that miR-93 can directly target PTEN and participates in the regulation of the AKT signaling pathway; MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues;Furthermore our study found berberine could inhibit miR-93 expression and function in ovarian cancer as shown by an increase of its target PTEN an important tumor suppressor in ovarian cancer; More importantly A2780 cells that were treated with PTEN siRNA had a survival pattern that is similar to cells with miR-93 overexpression ;tumorigenesis;;poor survival liver cancer;sarcoma;ovarian cancer;ovarian cancer hsa-let-7c-5p PTK2 -1.70744661345097 2.87934212005942e-20 0.592116034898139 1.42360483588582e-09 miRNAWalker2_validate -0.111526582483996 6.85487252665759e-06 NA NA NA hsa-miR-140-5p PTK2 -0.220106443070144 0.0140713197101242 0.592116034898139 1.42360483588582e-09 miRanda -0.143491653583679 0.00881810175828342 NA NA NA hsa-miR-193a-3p PTK2 -0.117675551589896 0.309391958208578 0.592116034898139 1.42360483588582e-09 miRNAWalker2_validate;miRanda -0.119325883264928 0.00471634522445647 NA NA NA hsa-miR-505-3p PTK2 -1.20027021591555 1.27927500175937e-13 0.592116034898139 1.42360483588582e-09 MirTarget;miRNATAP -0.118958314234155 3.90333340892144e-05 NA NA NA hsa-miR-590-3p PTK2 -0.473167498047808 1.76652934637227e-05 0.592116034898139 1.42360483588582e-09 miRanda -0.141997163320001 0.00120758249564418 NA NA NA hsa-miR-101-3p RAC1 -1.48176407980989 3.98950707385704e-31 0.216579201672401 0.00293326905874306 miRNAWalker2_validate;MirTarget -0.160619583159355 2.13421294167009e-10 25202416;24649082;25057058 In previous studies the role of miRNA-101 miR-101 in tumors has been identified as a tumor suppressor and until now the role of miR-101 and Rac1 in thyroid cancer has remained undefined; Rac1 was demonstrated to be negatively regulated by miR-101 at the post-transcriptional level via a specific target site within the 3' untranslated region by dual-luciferase reporter assay; The expression of Rac1 was also observed to inversely correlate with miR-101 expression in PTC tissues; knockdown of Rac1 by shRNA inhibited thyroid cancer cell migration and invasion resembling that of miR-101 overexpression; Thus these findings suggested that miR-101 acts as a novel suppressor by targeting the Rac1 gene and inhibiting thyroid cancer cell migration and invasion;miR 101 inhibits cell proliferation by targeting Rac1 in papillary thyroid carcinoma; In the present study we investigated the role of microRNA-101 miR-101 in PTC via targeting of Ras-related C3 botulinum toxin substrate 1 Rac1; Moreover algorithm-based and experimental strategies verified Rac1 as a direct target of miR-101 in the K1 cell line; Taken together these findings suggest that miR-101 inhibited PTC growth via the downregulation of Rac1 expression providing a better understanding of miRNA-modulated signaling networks for future cancer therapeutics;Mir-101 inhibitors significantly increased the luciferase activities of RAC1 3'UTR; Overexpression of mir-101 increased the expression of RAC1 while inhibition of mir-101 suppressed RAC1 expression; mir-101 can suppress RAC1 gene expression by targeting the specific sequence of RAC1 3'UTR cell migration;; thyroid cancer;thyroid cancer;colorectal cancer hsa-miR-106a-5p RBL2 -0.464229535269236 0.009724944358047 -0.988381422306337 9.82564074336519e-12 MirTarget;miRNATAP -0.117107890118936 0.00367936194107588 24045973 Among many miR-106a predicated target genes p130 RBL2 an retinoblastoma Rb tumor suppressor family member was not only confirmed as a specific target of miR-106a but also related to tumor growth and differentiation; The importance of mir-106a and RBL2 was further demonstrated in vivo in which SKOV3 cells overexpressing miR-106a formed poorly differentiated carcinomas and had reduced RBL2 levels differentiation ovarian cancer hsa-miR-106b-5p RBL2 0.648901865047099 8.24870862047885e-09 -0.988381422306337 9.82564074336519e-12 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.467612098017658 4.18657885507219e-14 NA NA NA hsa-miR-17-5p RBL2 0.698861147045461 2.43854106455398e-05 -0.988381422306337 9.82564074336519e-12 miRNAWalker2_validate;miRTarBase;MirTarget;TargetScan;miRNATAP -0.343656172428148 2.71737625593314e-16 NA NA NA hsa-miR-20a-5p RBL2 0.854326758727003 5.04256669663285e-07 -0.988381422306337 9.82564074336519e-12 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.264295882999639 1.60446558088321e-10 NA NA NA hsa-miR-335-3p RBL2 -0.275199247483111 0.106629165248783 -0.988381422306337 9.82564074336519e-12 MirTarget -0.114041268766779 0.00743241946989908 NA NA NA hsa-miR-421 RBL2 0.941856334694081 6.12167297054077e-08 -0.988381422306337 9.82564074336519e-12 miRanda -0.251090760557798 5.73687778774342e-10 NA NA NA hsa-miR-93-5p RBL2 1.39518349670953 2.84167728726712e-25 -0.988381422306337 9.82564074336519e-12 MirTarget;miRNATAP -0.408615196615982 9.43227929469049e-17 NA NA NA hsa-miR-128-3p RELN 0.289735108018712 0.0114419474207889 -2.82839358389431 4.74435506980363e-07 miRNAWalker2_validate;miRTarBase;MirTarget -0.767105353369543 0.00160868939358571 NA NA NA hsa-miR-15b-5p RELN 0.230566941413564 0.0824765001857479 -2.82839358389431 4.74435506980363e-07 MirTarget;miRNATAP -1.03951082038193 5.67902154561778e-07 NA NA NA hsa-miR-16-5p RELN -0.398577235179603 0.000101598495180191 -2.82839358389431 4.74435506980363e-07 MirTarget;miRNATAP -0.763446601483173 0.00489459284871768 NA NA NA hsa-miR-193a-5p RELN -0.472805720224262 1.24962968905481e-05 -2.82839358389431 4.74435506980363e-07 PITA -0.844977830578175 0.000949543214634947 NA NA NA hsa-miR-224-5p RELN 2.67175249172213 1.46995328399796e-18 -2.82839358389431 4.74435506980363e-07 MirTarget -0.270878865669266 0.0018905917611097 NA NA NA hsa-miR-331-5p RELN 0.213759185384356 0.120772399478646 -2.82839358389431 4.74435506980363e-07 MirTarget;miRNATAP -0.533411904806779 0.00877760936476095 NA NA NA hsa-miR-452-5p RELN 1.91544498799508 1.81995982853313e-14 -2.82839358389431 4.74435506980363e-07 MirTarget -0.413884885330082 0.000107564006914462 NA NA NA hsa-miR-542-3p RHEB -1.31351015149842 3.64642423288576e-22 0.594343190335174 8.4076096236034e-15 miRanda -0.154397416399753 5.84623305682461e-09 NA NA NA hsa-miR-99a-5p RPS6 -1.51481656384839 3.93230057259529e-14 0.185885010447493 0.120916808338817 miRNAWalker2_validate -0.141086052819295 4.22214050226356e-07 NA NA NA hsa-miR-126-5p RPS6KB1 -0.428561469405594 6.6035585844222e-05 0.0405513114293425 0.579068505304799 mirMAP -0.106946073918378 0.00114467289374143 NA NA NA hsa-miR-145-5p RPS6KB1 -1.4795885711477 6.47308361543414e-16 0.0405513114293425 0.579068505304799 MirTarget;miRNATAP -0.109724019103871 2.07298188703538e-09 24157791 MiR 145 is downregulated in human ovarian cancer and modulates cell growth and invasion by targeting p70S6K1 and MUC1; MiR-145 is found to negatively regulate P70S6K1 and MUC1 protein levels by directly targeting their 3'UTRs; Importantly the overexpression of p70S6K1 and MUC1 can restore the cell colony formation and invasion abilities that are reduced by miR-145 respectively; Our study suggests that miR-145 modulates ovarian cancer growth and invasion by suppressing p70S6K1 and MUC1 functioning as a tumor suppressor ovarian cancer hsa-miR-181a-5p RPS6KB1 0.250113435902954 0.0551933249319293 0.0405513114293425 0.579068505304799 MirTarget;miRNATAP -0.116671963226395 1.5972465565874e-05 NA NA NA hsa-miR-181c-5p RPS6KB1 -0.00639394152050299 0.969127212255928 0.0405513114293425 0.579068505304799 MirTarget;miRNATAP -0.10835890138279 3.360156885851e-07 NA NA NA hsa-miR-27a-3p RPS6KB1 -0.370807380415112 0.00875721956202713 0.0405513114293425 0.579068505304799 MirTarget;miRNATAP -0.13716383977936 2.72226167197111e-08 NA NA NA hsa-miR-342-3p RPS6KB1 -0.319677314984262 0.0449813878317147 0.0405513114293425 0.579068505304799 miRanda -0.115090266357129 1.55784431831661e-07 NA NA NA hsa-miR-542-3p RPS6KB1 -1.31351015149842 3.64642423288576e-22 0.0405513114293425 0.579068505304799 miRNATAP -0.102388373747613 3.28524227017976e-05 NA NA NA hsa-miR-125a-5p RPS6KB2 -0.91219969372382 1.38256246489548e-07 0.230775242252044 0.00570601482278892 miRanda -0.115997614156545 4.04919339586598e-07 NA NA NA hsa-miR-125a-5p RPTOR -0.91219969372382 1.38256246489548e-07 0.681970822272688 1.01116104311691e-17 mirMAP -0.143764013928447 1.57188124654312e-10 NA NA NA hsa-miR-24-3p RPTOR -0.257825594021966 0.00689742600215061 0.681970822272688 1.01116104311691e-17 mirMAP -0.149274897532169 0.000434319205332349 NA NA NA hsa-miR-27a-3p RPTOR -0.370807380415112 0.00875721956202713 0.681970822272688 1.01116104311691e-17 miRNAWalker2_validate -0.125149802279461 9.56293549288197e-06 NA NA NA hsa-miR-27b-3p RPTOR -0.823491817819754 9.20935302746823e-14 0.681970822272688 1.01116104311691e-17 miRNAWalker2_validate -0.116093691653188 0.001071699641235 NA NA NA hsa-miR-542-3p RPTOR -1.31351015149842 3.64642423288576e-22 0.681970822272688 1.01116104311691e-17 mirMAP -0.244667662489235 1.31330171903979e-19 NA NA NA hsa-miR-1976 RXRA -0.431472440543166 0.00324575010550946 -0.314271979229845 0.00905206396461103 MirTarget -0.129103197226646 0.00124010558710827 NA NA NA hsa-miR-342-3p RXRA -0.319677314984262 0.0449813878317147 -0.314271979229845 0.00905206396461103 miRanda -0.2016994638004 2.9357956390892e-08 NA NA NA hsa-miR-107 SGK1 0.241662461253461 0.0170788899505389 -1.41838635983173 4.94162348667021e-12 miRanda -0.316144022955829 0.00197325276655968 NA NA NA hsa-miR-15b-5p SGK1 0.230566941413564 0.0824765001857479 -1.41838635983173 4.94162348667021e-12 miRNATAP -0.243750750029338 0.00169253140185689 NA NA NA hsa-miR-361-5p SGK1 0.233202959858696 0.00961572812255327 -1.41838635983173 4.94162348667021e-12 miRanda -0.493410935282441 1.69010393254813e-05 NA NA NA hsa-miR-21-5p SGK3 1.51084295924322 1.54551622173259e-34 -0.235888881337767 0.0629928865889445 miRNAWalker2_validate -0.158120464091527 0.000607322860528501 NA NA NA hsa-miR-338-5p SGK3 -0.221898432884726 0.252390988727086 -0.235888881337767 0.0629928865889445 PITA;miRNATAP -0.103748211882523 0.00119443375751103 NA NA NA hsa-miR-16-1-3p SOS1 0.392360028038559 0.00112481918108198 0.05751534180893 0.493041822065916 mirMAP -0.102425524653968 0.00242014805702593 NA NA NA hsa-miR-17-5p SOS1 0.698861147045461 2.43854106455398e-05 0.05751534180893 0.493041822065916 MirTarget;TargetScan;miRNATAP -0.11660606180243 1.37071072811905e-06 NA NA NA hsa-miR-193a-3p SOS1 -0.117675551589896 0.309391958208578 0.05751534180893 0.493041822065916 miRanda -0.167375491584816 1.83548073403882e-06 NA NA NA hsa-miR-20a-5p SOS1 0.854326758727003 5.04256669663285e-07 0.05751534180893 0.493041822065916 MirTarget;miRNATAP -0.100063123806167 2.02695724421345e-05 NA NA NA hsa-miR-27a-3p SOS1 -0.370807380415112 0.00875721956202713 0.05751534180893 0.493041822065916 MirTarget;miRNATAP -0.120226883007702 2.62552416504337e-05 NA NA NA hsa-miR-30b-5p SOS1 -0.54056186708617 2.44688760798341e-05 0.05751534180893 0.493041822065916 mirMAP -0.125950231468023 6.21375879766061e-05 NA NA NA hsa-miR-339-5p SOS1 0.280589919878422 0.0355660282647215 0.05751534180893 0.493041822065916 miRNATAP -0.15315814967207 4.05818038840081e-07 NA NA NA hsa-miR-590-5p SOS1 -0.103355559980404 0.310027295317092 0.05751534180893 0.493041822065916 miRanda -0.115356122634493 0.00426551999929742 NA NA NA hsa-miR-146b-5p SOS2 0.419719546795449 0.0457375823220155 -0.326258424146275 0.000900714126323724 miRanda -0.110125845201631 1.10330856080798e-06 NA NA NA hsa-miR-21-5p SOS2 1.51084295924322 1.54551622173259e-34 -0.326258424146275 0.000900714126323724 miRNATAP -0.279899776012046 7.56477883436428e-16 NA NA NA hsa-miR-590-5p SOS2 -0.103355559980404 0.310027295317092 -0.326258424146275 0.000900714126323724 PITA;miRanda;miRNATAP -0.144457452099512 0.00241614674200376 NA NA NA hsa-miR-139-5p SPP1 -2.11299531796557 8.16407076415038e-28 1.86706198200398 0.000286687598509149 miRanda -1.1519772014133 2.46405732226498e-23 NA NA NA hsa-miR-335-5p SPP1 -1.61347836513558 1.62757083289711e-19 1.86706198200398 0.000286687598509149 miRNAWalker2_validate -0.389183181212584 0.00397841832397725 NA NA NA hsa-miR-130b-5p SYK 0.170599813152408 0.337611586432771 -0.590828623873433 0.00240111382935481 mirMAP -0.140609120016588 0.0087786689957213 NA NA NA hsa-miR-23b-3p SYK -0.530561587109023 5.43807206823652e-07 -0.590828623873433 0.00240111382935481 mirMAP -0.462565350806541 1.70759047452468e-07 NA NA NA hsa-miR-25-3p SYK 0.633062563556379 3.68686354834328e-10 -0.590828623873433 0.00240111382935481 mirMAP -0.294730516233669 0.00153194226766459 NA NA NA hsa-miR-32-5p SYK 0.0806126674664016 0.548984992497432 -0.590828623873433 0.00240111382935481 mirMAP -0.320398157589938 5.75043147590717e-06 NA NA NA hsa-miR-362-5p SYK 0.717921690799312 1.68392734504524e-05 -0.590828623873433 0.00240111382935481 mirMAP -0.146733432101884 0.00960568580224762 NA NA NA hsa-miR-532-5p SYK 1.02729546510601 9.93318913966636e-16 -0.590828623873433 0.00240111382935481 mirMAP -0.255126912271846 0.000386023023812825 NA NA NA hsa-miR-92a-3p SYK 0.212391012518195 0.134292268089997 -0.590828623873433 0.00240111382935481 mirMAP -0.211897034657997 0.00163947604157413 NA NA NA hsa-miR-421 TEK 0.941856334694081 6.12167297054077e-08 -2.10076794618928 8.11230955920562e-16 miRanda -0.557873847597563 1.84056043955124e-14 NA NA NA hsa-miR-140-3p TGFA 0.546012239205329 4.84578261098777e-11 -2.03762583735332 1.70833612877746e-11 miRNATAP -1.3315760430671 3.4498293194206e-14 NA NA NA hsa-miR-148a-3p TGFA -0.748702855596996 3.93313343865641e-07 -2.03762583735332 1.70833612877746e-11 miRNATAP -0.794888513212377 5.2082388955199e-16 NA NA NA hsa-miR-452-5p TGFA 1.91544498799508 1.81995982853313e-14 -2.03762583735332 1.70833612877746e-11 mirMAP -0.198820467771802 0.000663801168626612 NA NA NA hsa-miR-16-1-3p THBS1 0.392360028038559 0.00112481918108198 -2.1667874641621 3.47909831637717e-17 MirTarget -0.349993916076067 0.00122857732566393 NA NA NA hsa-miR-17-5p THBS1 0.698861147045461 2.43854106455398e-05 -2.1667874641621 3.47909831637717e-17 miRNAWalker2_validate -0.222517583477087 0.00443051973072606 NA NA NA hsa-miR-19a-3p THBS1 1.01712596153672 2.57753201450625e-07 -2.1667874641621 3.47909831637717e-17 MirTarget;mirMAP -0.266530076936376 3.91252800717626e-05 NA NA NA hsa-miR-19b-3p THBS1 0.601279551368824 0.00016685097085251 -2.1667874641621 3.47909831637717e-17 MirTarget;mirMAP -0.258570375211438 0.00144531729895803 NA NA NA hsa-miR-20a-5p THBS1 0.854326758727003 5.04256669663285e-07 -2.1667874641621 3.47909831637717e-17 miRNAWalker2_validate -0.298190202548282 7.75927048516442e-05 NA NA NA hsa-miR-32-5p THBS1 0.0806126674664016 0.548984992497432 -2.1667874641621 3.47909831637717e-17 mirMAP -0.415642500380362 1.79938285205236e-05 NA NA NA hsa-miR-589-3p THBS1 1.16846704028095 5.88525574424201e-11 -2.1667874641621 3.47909831637717e-17 mirMAP -0.207376170994383 0.00450904846984429 NA NA NA hsa-miR-616-5p THBS1 0.150569904638223 0.402836556304524 -2.1667874641621 3.47909831637717e-17 mirMAP -0.380879712809061 1.2036408171943e-07 NA NA NA hsa-miR-618 THBS1 0.13539895350352 0.517145913114103 -2.1667874641621 3.47909831637717e-17 MirTarget;PITA -0.212645935824045 0.000996351251570771 NA NA NA hsa-miR-92a-3p THBS1 0.212391012518195 0.134292268089997 -2.1667874641621 3.47909831637717e-17 miRNAWalker2_validate -0.25627633804456 0.00550821085297264 NA NA NA hsa-miR-106b-5p THBS2 0.648901865047099 8.24870862047885e-09 -0.502816786435579 0.158172197533798 MirTarget -0.486129671276457 0.00142005744712669 NA NA NA hsa-miR-17-5p THBS2 0.698861147045461 2.43854106455398e-05 -0.502816786435579 0.158172197533798 MirTarget;TargetScan -0.379943541096311 0.0002503795908654 NA NA NA hsa-miR-20a-5p THBS2 0.854326758727003 5.04256669663285e-07 -0.502816786435579 0.158172197533798 MirTarget -0.441793484244088 1.04186713814831e-05 NA NA NA hsa-miR-32-3p THBS2 0.219109440121582 0.207217051619543 -0.502816786435579 0.158172197533798 miRNATAP -0.641167195268276 2.66066595937387e-10 NA NA NA hsa-miR-590-5p THBS2 -0.103355559980404 0.310027295317092 -0.502816786435579 0.158172197533798 miRanda -0.711273245527382 3.36369868701585e-05 NA NA NA hsa-miR-7-1-3p THBS2 -0.569105143287799 1.85297665041424e-05 -0.502816786435579 0.158172197533798 MirTarget;mirMAP -0.343348786927578 0.00830467804380644 NA NA NA hsa-miR-93-5p THBS2 1.39518349670953 2.84167728726712e-25 -0.502816786435579 0.158172197533798 MirTarget -0.59037311789499 1.04093973646049e-06 NA NA NA hsa-miR-3170 THBS3 -0.490667754008914 0.0102869987033346 0.736359717922504 1.32735428268107e-09 MirTarget -0.147643618350093 3.26408755400288e-06 NA NA NA hsa-miR-326 THBS3 -1.881935132554 1.68087316863775e-21 0.736359717922504 1.32735428268107e-09 PITA -0.189238431181079 3.98819872990696e-11 NA NA NA hsa-miR-330-5p THBS3 0.442231058604099 0.0053308560843808 0.736359717922504 1.32735428268107e-09 PITA;miRNATAP -0.11211646631826 0.00311770883033679 NA NA NA hsa-miR-550a-5p THBS3 0.156224023171823 0.282505553619229 0.736359717922504 1.32735428268107e-09 MirTarget -0.188790759957711 5.05581870085321e-06 NA NA NA hsa-miR-142-3p THBS4 -1.4243101967815 1.92609481097765e-14 5.61005547523483 2.66115801949053e-37 miRanda -1.10948136769617 1.72596454471113e-21 NA NA NA hsa-miR-26b-3p THEM4 -1.25503731613309 4.45390890119649e-27 0.553398288098321 1.69155816231868e-08 MirTarget -0.104565215685861 0.00769199111827087 NA NA NA hsa-miR-30a-5p THEM4 -0.626257932887855 0.000108198196973765 0.553398288098321 1.69155816231868e-08 miRNAWalker2_validate -0.151748928173726 2.66541990770991e-07 NA NA NA hsa-miR-107 TLR4 0.241662461253461 0.0170788899505389 -1.6828344992865 2.89483284631908e-26 miRanda -0.313089132572099 0.000126839900553517 NA NA NA hsa-miR-17-5p TLR4 0.698861147045461 2.43854106455398e-05 -1.6828344992865 2.89483284631908e-26 TargetScan -0.322592317634545 2.51351974300813e-11 NA NA NA hsa-miR-21-5p TLR4 1.51084295924322 1.54551622173259e-34 -1.6828344992865 2.89483284631908e-26 miRNAWalker2_validate -0.447384108410163 8.62007595677013e-14 27286259 For in vivo relevance expression of TLR4 was correlated with miR-21 expression and ROS production in freshly isolated untreated primary human lung cancer cells lung cancer hsa-miR-421 TLR4 0.941856334694081 6.12167297054077e-08 -1.6828344992865 2.89483284631908e-26 miRanda -0.208364346121929 7.58859321383203e-06 NA NA NA hsa-miR-365a-3p TNC 0.164591326307714 0.15325084963213 -0.741405240029029 0.00843299261104527 MirTarget -0.507909356836929 2.14253613517559e-05 NA NA NA hsa-miR-1301-3p TNXB 1.12156714169769 4.22404125385203e-10 -1.80921595703393 1.43653387554758e-16 miRNAWalker2_validate -0.186417244545931 0.00204447838050052 NA NA NA hsa-miR-122-5p TP53 -1.23907441373955 4.42181244888642e-06 -0.163823087975333 0.268013799235932 miRNATAP -0.104401376732605 6.76735273123785e-05 23221562;22235305 Loss of miR-122 expression in patients with hepatitis B enhances hepatitis B virus replication through cyclin G1-modulated P53 activity.;Surprisingly miR-122 overexpression decreased the sensitivity to the chemotherapy-induced apoptosis via a signaling circuit involving the activation of Akt and inhibition of p53 ; liver cancer;lymphoma hsa-miR-25-3p TP53 0.633062563556379 3.68686354834328e-10 -0.163823087975333 0.268013799235932 miRNAWalker2_validate -0.198477612132015 0.00480268903724675 22431589 The results define miR-25 and -32 as positive regulators of p53 underscoring their role in tumorigenesis in glioblastoma tumorigenesis glioblastoma hsa-miR-10a-5p TSC1 -1.47723516092033 7.27299669108673e-16 0.920881916944059 1.33648384372723e-19 mirMAP -0.139629452664737 2.24435503048579e-07 NA NA NA hsa-miR-139-5p TSC1 -2.11299531796557 8.16407076415038e-28 0.920881916944059 1.33648384372723e-19 mirMAP -0.138969507847749 1.79604322012115e-08 NA NA NA hsa-miR-142-3p TSC1 -1.4243101967815 1.92609481097765e-14 0.920881916944059 1.33648384372723e-19 miRanda -0.126618039733337 2.13468537583732e-06 NA NA NA hsa-miR-195-3p TSC1 -1.09375690196245 4.70092324819807e-09 0.920881916944059 1.33648384372723e-19 mirMAP -0.102350213332177 0.000197504018678128 NA NA NA hsa-miR-335-5p TSC1 -1.61347836513558 1.62757083289711e-19 0.920881916944059 1.33648384372723e-19 miRNAWalker2_validate -0.127585694708462 3.44981987359883e-06 NA NA NA hsa-miR-3614-5p TSC1 -1.66311416965679 1.26647181860439e-15 0.920881916944059 1.33648384372723e-19 mirMAP -0.106465716187074 1.0480173495806e-05 NA NA NA hsa-miR-450b-5p TSC1 -1.34333360369432 2.68386079790462e-17 0.920881916944059 1.33648384372723e-19 mirMAP -0.130360378995634 2.9321967866797e-05 NA NA NA hsa-miR-542-3p TSC1 -1.31351015149842 3.64642423288576e-22 0.920881916944059 1.33648384372723e-19 PITA;miRanda -0.198207156237058 3.1659040994906e-08 NA NA NA hsa-miR-744-5p TSC2 -0.378868377317939 0.00125071229180016 0.454638169130336 1.34709422274577e-05 miRNAWalker2_validate -0.146650115106937 0.000784576818042153 NA NA NA hsa-miR-150-5p VEGFA -1.14624309254329 1.51365525978726e-06 0.0263183839451075 0.81382635000524 miRNAWalker2_validate;miRTarBase -0.114666527453602 2.4663414134099e-07 NA NA NA hsa-miR-29b-2-5p VEGFA -0.223258653034772 0.084306146519938 0.0263183839451075 0.81382635000524 MirTarget -0.132006150652748 0.00171675395806506 NA NA NA hsa-miR-29b-3p VEGFA -0.348885528720517 0.0121441341291285 0.0263183839451075 0.81382635000524 miRNAWalker2_validate;miRTarBase;MirTarget -0.125790856471025 0.00126089778683366 26155940;22815235 Moreover miR-29b inhibits angiogenesis by attenuating tube formation and the expression of VEGF and Ang-2 and stemness maintenance in GBM cells as demonstrated by decreasing neurosphere formation and cancer stem cell marker protein expression;Additionally for two genes that are deregulated in PCa heterogeneous nuclear ribonucleoprotein K hnRNP-K and vascular endothelial growth factor A VEGF-A we identified two regulatory miRNAs miR-205 and miR-29b ; glioblastoma;prostate cancer hsa-miR-128-3p VEGFC 0.289735108018712 0.0114419474207889 0.0031169028254141 0.982787493023474 miRNAWalker2_validate;MirTarget -0.198618366596319 0.00124097531466772 25001183;26722309 Interestingly ectopic miR-128 overexpression could significantly inhibit vascular endothelial growth factor VEGF-C expression and reduce the activity of a luciferase reporter containing the VEGF-C 3'-untranslated region; These findings suggest that miR-128 could play a role in NSCLC tumourigenesis at least in part by modulation of angiogenesis and lymphangiogenesis through targeting VEGF-C and could simultaneously block ERK AKT and p38 signalling pathways;miR 128 downregulation promotes growth and metastasis of bladder cancer cells and involves VEGF C upregulation; To investigate the regulation of miR-128 on VEGF-C expression and their effects on proliferation and metastasis of bladder cancer T24 and 5637 BC cells were transfected with pre-miR-128 anti-miR-128 and their respective negative control; miR-128 was downregulated in BC tissues and cell lines while the expression levels of VEGF-C were upregulated; The present results indicated that miR-128 negatively regulated VEGF-C expression in BC T24 and 5637 BC cells; VEGF-C is a direct target of miR-128 in BC cells ;metastasis lung squamous cell cancer;bladder cancer hsa-miR-16-2-3p VEGFC -0.0336420826463706 0.805158952566973 0.0031169028254141 0.982787493023474 MirTarget -0.149514000508957 0.00387572506927192 NA NA NA hsa-miR-374a-5p VEGFC 0.016390363747302 0.869784217438621 0.0031169028254141 0.982787493023474 MirTarget -0.28522635078723 5.53265846690998e-05 NA NA NA hsa-miR-374b-5p VEGFC -0.307538028546424 0.0030079617545972 0.0031169028254141 0.982787493023474 MirTarget -0.209454919974677 0.00209421071373184 NA NA NA hsa-miR-590-3p VEGFC -0.473167498047808 1.76652934637227e-05 0.0031169028254141 0.982787493023474 miRanda -0.274821384359749 1.36978001441178e-05 NA NA NA hsa-miR-15b-5p VWF 0.230566941413564 0.0824765001857479 1.44034219749898 1.02989206792567e-11 mirMAP -0.32225679373812 5.25271783609621e-05 NA NA NA hsa-miR-16-5p VWF -0.398577235179603 0.000101598495180191 1.44034219749898 1.02989206792567e-11 mirMAP -0.312233093103415 0.00253525685553441 NA NA NA hsa-miR-424-5p VWF -2.62653257498508 2.38997974737991e-50 1.44034219749898 1.02989206792567e-11 mirMAP -0.240836784366552 4.8746962377372e-06 NA NA NA hsa-miR-429 VWF -1.40262535827285 6.93982219171912e-05 1.44034219749898 1.02989206792567e-11 miRNATAP -0.212842066643388 4.09541289741096e-13 NA NA NA hsa-miR-107 YWHAB 0.241662461253461 0.0170788899505389 0.173432047741129 0.000884243127746855 miRanda -0.108037306925883 1.47087762859095e-05 NA NA NA hsa-miR-148a-3p YWHAB -0.748702855596996 3.93313343865641e-07 0.173432047741129 0.000884243127746855 MirTarget;miRNATAP -0.132820922282045 4.13292521804208e-16 NA NA NA hsa-miR-186-5p YWHAE -0.060103391373687 0.535288717081205 -0.389050776848833 1.87949073175552e-07 miRNAWalker2_validate -0.127528287333916 0.000863275542227347 NA NA NA hsa-miR-192-3p YWHAH -0.6401165114007 0.000266272092403814 0.381424797266773 9.9564983661589e-07 MirTarget -0.123473061996178 7.45881423647248e-09 NA NA NA hsa-miR-139-5p YWHAQ -2.11299531796557 8.16407076415038e-28 0.105106147496931 0.185320360738181 MirTarget;PITA;miRanda;miRNATAP -0.134903753655919 8.26465948813414e-14 NA NA NA hsa-miR-140-5p YWHAQ -0.220106443070144 0.0140713197101242 0.105106147496931 0.185320360738181 miRanda -0.114458361490446 0.00840912415391188 NA NA NA hsa-miR-328-3p YWHAQ -0.547466865311955 6.8359574451572e-06 0.105106147496931 0.185320360738181 miRNAWalker2_validate -0.117573761953222 0.000175415891769642 NA NA NA hsa-miR-497-5p YWHAQ -1.41371173021948 7.09278946477743e-17 0.105106147496931 0.185320360738181 MirTarget;miRNATAP -0.10611692853337 9.41242986957199e-07 NA NA NA hsa-miR-193b-3p YWHAZ -0.166697207281748 0.272022543069482 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate;miRTarBase;MirTarget;miRNATAP -0.215846083623554 1.05246240233955e-09 NA NA NA hsa-miR-195-3p YWHAZ -1.09375690196245 4.70092324819807e-09 0.521348507448096 2.46588555709085e-06 MirTarget -0.123410355780779 1.86763692080896e-05 NA NA NA hsa-miR-22-3p YWHAZ -0.628561621952407 1.33755558015523e-09 0.521348507448096 2.46588555709085e-06 MirTarget -0.422402725731077 3.08582185459414e-17 NA NA NA hsa-miR-28-5p YWHAZ -0.430964830599964 2.67367257252925e-06 0.521348507448096 2.46588555709085e-06 PITA;miRanda;miRNATAP -0.378439288562354 7.80189740644095e-11 NA NA NA hsa-miR-30a-5p YWHAZ -0.626257932887855 0.000108198196973765 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate;MirTarget;miRNATAP -0.137637850164864 3.55605716079781e-05 NA NA NA hsa-miR-30c-5p YWHAZ -0.432111642767632 0.000159764714931007 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.217149970191143 4.21662119138978e-06 24519092 Involvement of miR 30c in resistance to doxorubicin by regulating YWHAZ in breast cancer cells; Together our findings provided evidence that miR-30c was one of the important miRNAs in doxorubicin resistance by regulating YWHAZ in the breast cancer cell line MCF-7/ADR drug resistance breast cancer hsa-miR-30e-5p YWHAZ -0.627815042856657 3.02205690184908e-10 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.512830738492514 1.08747499679871e-23 NA NA NA hsa-miR-328-3p YWHAZ -0.547466865311955 6.8359574451572e-06 0.521348507448096 2.46588555709085e-06 MirTarget -0.184846832867037 2.98681633637662e-05 NA NA NA hsa-miR-340-3p YWHAZ -0.329393899588902 0.00329627776838916 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate -0.145643740083093 0.00280561227887093 NA NA NA hsa-miR-374a-3p YWHAZ -0.207171354826929 0.0623494608399903 0.521348507448096 2.46588555709085e-06 mirMAP -0.243786914759101 6.00089907045834e-07 NA NA NA hsa-miR-378a-3p YWHAZ -1.1920055906972 3.23538871733634e-10 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate -0.229761242047093 2.06324870493417e-17 NA NA NA hsa-miR-451a YWHAZ -2.1025161666445 6.82555767537341e-17 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.125160799099819 8.46499537092129e-10 26885274 miR 451 regulates FoxO3 nuclear accumulation through Ywhaz in human colorectal cancer; In this study expressions of miR-451 and Ywhaz in CRC tissues and adjacent normal tissues were detected by quantitative real-time PCR qRT-PCR and immunohistochemistry respectively; Ywhaz-3'UTR luciferase reporter assay confirmed Ywhaz as a direct target gene of miR-451; miR-451 may inhibit the colon cancer growth in vitro and in vivo likely through directly targeting Ywhaz and indirectly regulating the nuclear accumulation of FoxO3 colorectal cancer hsa-miR-455-3p YWHAZ -1.3998033821095 5.72010709806871e-17 0.521348507448096 2.46588555709085e-06 miRNAWalker2_validate -0.163253715474292 1.350262159478e-07 NA NA NA hsa-miR-505-5p YWHAZ -0.768282569967001 1.14662846595407e-05 0.521348507448096 2.46588555709085e-06 MirTarget;miRNATAP -0.138532797418727 5.93157816903527e-06 NA NA NA hsa-miR-576-5p YWHAZ -0.377452008285847 0.00470735657343984 0.521348507448096 2.46588555709085e-06 mirMAP -0.244889551272521 1.02393670706879e-09 NA NA NA hsa-miR-664a-3p YWHAZ 0.485807855553031 0.000732553249798598 0.521348507448096 2.46588555709085e-06 mirMAP -0.120325216996267 0.00142367135354135 NA NA NA hsa-miR-7-1-3p YWHAZ -0.569105143287799 1.85297665041424e-05 0.521348507448096 2.46588555709085e-06 mirMAP -0.154608457268063 0.000140054345497285 NA NA NA