TISIDB

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Endoplasmic reticulum membrane Single-pass type III membrane protein
Domain	PF01693 Caulimovirus viroplasmin PF03020 LEM domain
Function	Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Involved in brain development (PubMed:25259927).

> Gene Ontology [ TOP ]
Biological Process	GO:0006470 protein dephosphorylation GO:0006997 nucleus organization GO:0006998 nuclear envelope organization GO:0007067 mitotic nuclear division GO:0007084 mitotic nuclear envelope reassembly GO:0010921 regulation of phosphatase activity GO:0016311 dephosphorylation GO:0031468 nuclear envelope reassembly GO:0034047 regulation of protein phosphatase type 2A activity GO:0035303 regulation of dephosphorylation GO:0035304 regulation of protein dephosphorylation GO:0035306 positive regulation of dephosphorylation GO:0035307 positive regulation of protein dephosphorylation GO:0042326 negative regulation of phosphorylation GO:0043666 regulation of phosphoprotein phosphatase activity
Molecular Function	GO:0008601 protein phosphatase type 2A regulator activity GO:0019208 phosphatase regulator activity GO:0019888 protein phosphatase regulator activity GO:0019902 phosphatase binding GO:0019903 protein phosphatase binding GO:0051721 protein phosphatase 2A binding
Cellular Component	GO:0030176 integral component of endoplasmic reticulum membrane GO:0031227 intrinsic component of endoplasmic reticulum membrane

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	R-HSA-1640170: Cell Cycle R-HSA-69278: Cell Cycle, Mitotic R-HSA-2995383: Initiation of Nuclear Envelope Reformation R-HSA-68886: M Phase R-HSA-68882: Mitotic Anaphase R-HSA-2555396: Mitotic Metaphase and Anaphase R-HSA-2995410: Nuclear Envelope Reassembly

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between ANKLE2 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of ANKLE2 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	Second most enriched score: 0.75	Sensitive to T cell-mediated killing
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the expression difference of ANKLE2 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	-0.046	0.819
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.055	0.977
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.035	0.978
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	-0.064	0.812
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	-0.077	0.975
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	-0.045	0.989
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	-0.035	0.929
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	-0.072	0.967
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.041	0.983
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	0.632	0.655
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.965	0.651
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.064	0.292

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of ANKLE2 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	11.8	-11.8	0.488
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	33.3	-33.3	0.231
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	7.1	-7.1	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	3.7	4.1	-0.4	1
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	7.1	-7.1	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	3.7	3.4	0.3	1
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	4.8	0	4.8	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	7.7	0	7.7	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	6.2	-6.2	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	14.3	-14.3	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	2.6	3.7	-1.1	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	4.5	7.7	-3.2	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	7.7	-7.7	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	8.3	-8.3	1

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of ANKLE2. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of ANKLE2. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by ANKLE2. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of ANKLE2. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of ANKLE2 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between ANKLE2 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	ANKLE2
Name	ankyrin repeat and LEM domain containing 2
Aliases	LEMD7; Lem4; LEM domain containing 7; KIAA0692; LEM domain-containing protein 4; Ankyrin repeat and LEM doma ......
Chromosomal Location	12q24.33
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting ANKLE2 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

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