TISIDB

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Secreted, extracellular space, extracellular matrix, basement membrane. Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement membrane (TBM) and synaptic basal lamina (BL).
Domain	PF01413 C-terminal tandem repeated domain in type 4 procollagen PF01391 Collagen triple helix repeat (20 copies)
Function	Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; FUNCTION: Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity; these two anti-tumor properties may be regulated via RGD-independent ITGB3-mediated mechanisms.

> Gene Ontology [ TOP ]
Biological Process	GO:0001525 angiogenesis GO:0001655 urogenital system development GO:0001822 kidney development GO:0003013 circulatory system process GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process GO:0007605 sensory perception of sound GO:0008015 blood circulation GO:0009743 response to carbohydrate GO:0009746 response to hexose GO:0009749 response to glucose GO:0010466 negative regulation of peptidase activity GO:0010950 positive regulation of endopeptidase activity GO:0010951 negative regulation of endopeptidase activity GO:0010952 positive regulation of peptidase activity GO:0016525 negative regulation of angiogenesis GO:0030198 extracellular matrix organization GO:0030574 collagen catabolic process GO:0032835 glomerulus development GO:0032836 glomerular basement membrane development GO:0032963 collagen metabolic process GO:0034284 response to monosaccharide GO:0043062 extracellular structure organization GO:0043280 positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043281 regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0044236 multicellular organism metabolic process GO:0044243 multicellular organism catabolic process GO:0044259 multicellular organismal macromolecule metabolic process GO:0045765 regulation of angiogenesis GO:0045861 negative regulation of proteolysis GO:0045862 positive regulation of proteolysis GO:0048514 blood vessel morphogenesis GO:0050954 sensory perception of mechanical stimulus GO:0051346 negative regulation of hydrolase activity GO:0052547 regulation of peptidase activity GO:0052548 regulation of endopeptidase activity GO:0072001 renal system development GO:0072006 nephron development GO:0072577 endothelial cell apoptotic process GO:1901342 regulation of vasculature development GO:1901343 negative regulation of vasculature development GO:1904019 epithelial cell apoptotic process GO:2000116 regulation of cysteine-type endopeptidase activity GO:2000181 negative regulation of blood vessel morphogenesis GO:2001056 positive regulation of cysteine-type endopeptidase activity
Molecular Function	GO:0004857 enzyme inhibitor activity GO:0004866 endopeptidase inhibitor activity GO:0005178 integrin binding GO:0005201 extracellular matrix structural constituent GO:0008191 metalloendopeptidase inhibitor activity GO:0030414 peptidase inhibitor activity GO:0050839 cell adhesion molecule binding GO:0061134 peptidase regulator activity GO:0061135 endopeptidase regulator activity
Cellular Component	GO:0005578 proteinaceous extracellular matrix GO:0005581 collagen trimer GO:0005587 collagen type IV trimer GO:0005604 basement membrane GO:0005788 endoplasmic reticulum lumen GO:0044420 extracellular matrix component GO:0098642 network-forming collagen trimer GO:0098644 complex of collagen trimers GO:0098645 collagen network GO:0098651 basement membrane collagen trimer

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa04151 PI3K-Akt signaling pathway hsa04510 Focal adhesion hsa04512 ECM-receptor interaction hsa04974 Protein digestion and absorption
Reactome	R-HSA-2022090: Assembly of collagen fibrils and other multimeric structures R-HSA-422475: Axon guidance R-HSA-1650814: Collagen biosynthesis and modifying enzymes R-HSA-8948216: Collagen chain trimerization R-HSA-1474290: Collagen formation R-HSA-1266738: Developmental Biology R-HSA-1474244: Extracellular matrix organization R-HSA-216083: Integrin cell surface interactions R-HSA-375165: NCAM signaling for neurite out-growth R-HSA-419037: NCAM1 interactions R-HSA-162582: Signal Transduction R-HSA-186797: Signaling by PDGF

> KEGG and Reactome Pathway

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KEGG

hsa04151 PI3K-Akt signaling pathway
hsa04510 Focal adhesion
hsa04512 ECM-receptor interaction
hsa04974 Protein digestion and absorption

Reactome

R-HSA-2022090: Assembly of collagen fibrils and other multimeric structures
R-HSA-422475: Axon guidance
R-HSA-1650814: Collagen biosynthesis and modifying enzymes
R-HSA-8948216: Collagen chain trimerization
R-HSA-1474290: Collagen formation
R-HSA-1266738: Developmental Biology
R-HSA-1474244: Extracellular matrix organization
R-HSA-216083: Integrin cell surface interactions
R-HSA-375165: NCAM signaling for neurite out-growth
R-HSA-419037: NCAM1 interactions
R-HSA-162582: Signal Transduction
R-HSA-186797: Signaling by PDGF

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between COL4A3 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

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Statistical results of COL4A3 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of COL4A3 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0	1
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	0	1
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	0	1
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0.515	0.293
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.835	0.68
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0.107	0.966
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	-0.113	0.841
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.208	0.8
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	-0.335	0.705
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	-0.362	0.662
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.241	0.823
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.066	0.841

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of COL4A3 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	5.9	-5.9	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	33.3	-33.3	0.231
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	22.2	11	11.2	0.194
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	7.1	-7.1	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	22.2	11.9	10.3	0.33
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	42.9	17.6	25.3	0.161
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	25	33.3	-8.3	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	53.8	9.1	44.7	0.0335
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	18.4	0	18.4	0.0356
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	22.7	0	22.7	0.134
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	12.5	0	12.5	0.485
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	0	1

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of COL4A3. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of COL4A3. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by COL4A3. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of COL4A3. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of COL4A3 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between COL4A3 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	COL4A3
Name	collagen, type IV, alpha 3 (Goodpasture antigen)
Aliases	tumstatin; collagen IV, alpha-3 polypeptide; Goodpasture antigen; Collagen alpha-3(IV) chain
Chromosomal Location	2q36-q37
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting COL4A3 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse COL4A3