TISIDB

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm Nucleus Note=Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity).
Domain	PF00270 DEAD/DEAH box helicase PF00271 Helicase conserved C-terminal domain
Function	ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).

> Gene Ontology [ TOP ]
Biological Process	GO:0006403 RNA localization GO:0006405 RNA export from nucleus GO:0006406 mRNA export from nucleus GO:0006417 regulation of translation GO:0006913 nucleocytoplasmic transport GO:0007281 germ cell development GO:0007283 spermatogenesis GO:0007286 spermatid development GO:0010501 RNA secondary structure unwinding GO:0010608 posttranscriptional regulation of gene expression GO:0015931 nucleobase-containing compound transport GO:0022412 cellular process involved in reproduction in multicellular organism GO:0034248 regulation of cellular amide metabolic process GO:0048232 male gamete generation GO:0048515 spermatid differentiation GO:0050657 nucleic acid transport GO:0050658 RNA transport GO:0051028 mRNA transport GO:0051168 nuclear export GO:0051169 nuclear transport GO:0051236 establishment of RNA localization GO:0071166 ribonucleoprotein complex localization GO:0071426 ribonucleoprotein complex export from nucleus GO:0071427 mRNA-containing ribonucleoprotein complex export from nucleus
Molecular Function	GO:0003724 RNA helicase activity GO:0004004 ATP-dependent RNA helicase activity GO:0004386 helicase activity GO:0008026 ATP-dependent helicase activity GO:0008186 RNA-dependent ATPase activity GO:0016887 ATPase activity GO:0042623 ATPase activity, coupled GO:0070035 purine NTP-dependent helicase activity
Cellular Component	GO:0033391 chromatoid body GO:0035770 ribonucleoprotein granule GO:0036464 cytoplasmic ribonucleoprotein granule

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	-

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between DDX25 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of DDX25 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the expression difference of DDX25 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	-0.401	0.306
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.77	0.204
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.151	0.784
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0.164	0.805
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.319	0.788
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	-0.043	0.973
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	-0.308	0.716
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.45	0.69
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	-0.663	0.609
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	-0.092	0.857
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.169	0.817
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.667	0.00696

> Mutation difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the mutation difference of DDX25 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	7.1	5.9	1.2	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	10	33.3	-23.3	0.423
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	0	0	0	1
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	0	0	0	1
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	4.8	0	4.8	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	7.7	0	7.7	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	2.6	0	2.6	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	4.5	0	4.5	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	0	1

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of DDX25. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of DDX25. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by DDX25. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of DDX25. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of DDX25 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between DDX25 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	DDX25
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 25
Aliases	gonadotropin-regulated testicular RNA helicase; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25; DEAD (Asp-G ......
Chromosomal Location	11q24
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting DDX25 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse DDX25