TISIDB

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm. Nucleus speckle. Nucleus, Cajal body. Note=Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.
Domain	PF00270 DEAD/DEAH box helicase PF00271 Helicase conserved C-terminal domain
Function	ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.

> Gene Ontology [ TOP ]
Biological Process	GO:0010501 RNA secondary structure unwinding
Molecular Function	GO:0003724 RNA helicase activity GO:0004004 ATP-dependent RNA helicase activity GO:0004386 helicase activity GO:0008026 ATP-dependent helicase activity GO:0008186 RNA-dependent ATPase activity GO:0016887 ATPase activity GO:0042623 ATPase activity, coupled GO:0070035 purine NTP-dependent helicase activity
Cellular Component	GO:0015030 Cajal body GO:0016604 nuclear body GO:0016607 nuclear speck

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa03040 Spliceosome
Reactome	R-HSA-74160: Gene Expression R-HSA-72203: Processing of Capped Intron-Containing Pre-mRNA R-HSA-72172: mRNA Splicing R-HSA-72163: mRNA Splicing - Major Pathway R-HSA-72165: mRNA Splicing - Minor Pathway

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between DDX42 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of DDX42 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	logFC: 0.85; FDR: 0.000651	Sensitive to T cell-mediated killing
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	logFC: -2.35; FDR: 0.00722	Resistant to T cell-mediated killing
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the expression difference of DDX42 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.064	0.701
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.068	0.977
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	0.159	0.927
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0.17	0.582
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.067	0.979
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0.304	0.927
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.085	0.84
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.032	0.987
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.152	0.945
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	-0.004	0.998
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.645	0.798
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.026	0.52

> Mutation difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the mutation difference of DDX42 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	7.4	7.4	0.0709
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	7.4	7.4	0.096
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	0	0	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	5.3	5.3	0.507
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	4.5	4.5	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	6.2	6.2	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	1

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of DDX42. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of DDX42. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by DDX42. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of DDX42. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of DDX42 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between DDX42 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	DDX42
Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Aliases	RNAHP; RHELP; SF3b125; SF3B8; splicing factor 3b, subunit 8; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DDX4 ......
Chromosomal Location	17q23
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting DDX42 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse DDX42