TISIDB

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm
Domain	PF02350 UDP-N-acetylglucosamine 2-epimerase PF00480 ROK family
Function	Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development (By similarity). Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.

> Gene Ontology [ TOP ]
Biological Process	GO:0006040 amino sugar metabolic process GO:0006044 N-acetylglucosamine metabolic process GO:0006045 N-acetylglucosamine biosynthetic process GO:0006047 UDP-N-acetylglucosamine metabolic process GO:0006054 N-acetylneuraminate metabolic process GO:0009225 nucleotide-sugar metabolic process GO:0044262 cellular carbohydrate metabolic process GO:0046349 amino sugar biosynthetic process GO:0046835 carbohydrate phosphorylation GO:1901071 glucosamine-containing compound metabolic process GO:1901073 glucosamine-containing compound biosynthetic process
Molecular Function	GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds GO:0008761 UDP-N-acetylglucosamine 2-epimerase activity GO:0009384 N-acylmannosamine kinase activity GO:0016798 hydrolase activity, acting on glycosyl bonds GO:0016853 isomerase activity GO:0016854 racemase and epimerase activity GO:0016857 racemase and epimerase activity, acting on carbohydrates and derivatives GO:0019200 carbohydrate kinase activity
Cellular Component	-

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa00520 Amino sugar and nucleotide sugar metabolism hsa01100 Metabolic pathways
Reactome	R-HSA-446203: Asparagine N-linked glycosylation R-HSA-446193: Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein R-HSA-392499: Metabolism of proteins R-HSA-597592: Post-translational protein modification R-HSA-4085001: Sialic acid metabolism R-HSA-446219: Synthesis of substrates in N-glycan biosythesis

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between GNE and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of GNE in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	logFC: -2.06; FDR: 0.04630	Resistant to T cell-mediated killing
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	logFC: -2.27; FDR: 0.02150	Resistant to T cell-mediated killing
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	STARS Score: 3.96; FDR: 0.025	Resistant to T cell-mediated killing
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of GNE in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.241	0.429
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	0.71	0.621
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.094	0.922
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0.156	0.533
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.045	0.981
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0.298	0.903
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.085	0.83
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	-0.16	0.915
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.355	0.831
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	0.283	0.786
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.803	0.584
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.212	0.108

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of GNE in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	0	1.4	-1.4	1
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	0	1.7	-1.7	1
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	0	0	0	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	2.6	0	2.6	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	4.5	0	4.5	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	0	1

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of GNE. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of GNE. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by GNE. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of GNE. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of GNE expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between GNE and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	GNE
Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Aliases	Uae1; IBM2; UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase; DMRV; GLCNE; N-acylmannosamine k ......
Chromosomal Location	9p13.1
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting GNE collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

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