TISIDB

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Membrane Peripheral membrane protein Secreted
Domain	PF00008 EGF-like domain PF00754 F5/8 type C domain
Function	Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. ; FUNCTION: Medin is the main constituent of aortic medial amyloid.

> Gene Ontology [ TOP ]
Biological Process	GO:0001525 angiogenesis GO:0006909 phagocytosis GO:0006910 phagocytosis, recognition GO:0006911 phagocytosis, engulfment GO:0007338 single fertilization GO:0008037 cell recognition GO:0009566 fertilization GO:0010324 membrane invagination GO:0030100 regulation of endocytosis GO:0043277 apoptotic cell clearance GO:0045807 positive regulation of endocytosis GO:0048514 blood vessel morphogenesis GO:0050764 regulation of phagocytosis GO:0050766 positive regulation of phagocytosis GO:0060627 regulation of vesicle-mediated transport GO:0099024 plasma membrane invagination GO:2000425 regulation of apoptotic cell clearance GO:2000427 positive regulation of apoptotic cell clearance
Molecular Function	GO:0001786 phosphatidylserine binding GO:0005178 integrin binding GO:0005543 phospholipid binding GO:0008429 phosphatidylethanolamine binding GO:0050839 cell adhesion molecule binding GO:0072341 modified amino acid binding
Cellular Component	GO:0009897 external side of plasma membrane GO:0019897 extrinsic component of plasma membrane GO:0019898 extrinsic component of membrane GO:0098552 side of membrane

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	R-HSA-977225: Amyloid fiber formation R-HSA-392499: Metabolism of proteins

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between MFGE8 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining

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Literatures describing the relation between MFGE8 and anti-tumor immunity in human cancer.

PMID	Cancer type	Relation to immunity	Evidence sentences
19433619	Colon carcinoma	Inhibit immunity	Within the tumor microenvironment, the secreted protein milk fat globule epidermal growth factor-8 (MFG-E8) stimulates disease progression through coordinated alpha(v)beta(3) integrin signaling in tumor and host cells. MFG-E8 enhances tumor cell survival, invasion, and angiogenesis, and contributes to local immune suppression. Collectively, these findings suggest that systemic MFG-E8 blockade might intensify the antitumor activities of existing therapeutic regimens through coordinated cell-autonomous and immune-mediated mechanisms.
18974133	Melanoma	Inhibit immunity	Milk fat globule EGF-8 promotes melanoma progression through coordinated Akt and twist signaling in the tumor microenvironment. In human melanoma cells, MFG-E8 knockdown attenuated Akt and Twist signaling and thereby compromised tumor cell survival, EMT, and invasive ability. MFG-E8-deficient human melanoma cells also showed increased sensitivity to small molecule inhibitors of insulin-like growth factor I receptor and c-Met.

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

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Statistical results of MFGE8 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of MFGE8 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.166	0.684
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.073	0.984
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	0.344	0.894
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	-0.227	0.605
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	-0.473	0.822
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0.088	0.975
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.201	0.7
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.334	0.874
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.01	0.997
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	1.205	0.566
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	2.607	0.369
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	-0.375	0.0029

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of MFGE8 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	0	4.1	-4.1	0.561
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	7.1	-7.1	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	0	3.4	-3.4	1
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	4.8	0	4.8	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	12.5	0	12.5	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	6.2	-6.2	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	14.3	-14.3	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	2.6	0	2.6	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	0	0	0	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	6.2	0	6.2	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	0	1

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of MFGE8. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of MFGE8. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by MFGE8. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of MFGE8. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of MFGE8 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between MFGE8 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	MFGE8
Name	milk fat globule-EGF factor 8 protein
Aliases	SED1; EDIL1; BA46; OAcGD3S; HsT19888; MFG-E8; hP47; sperm surface protein hP47; SPAG10; sperm associated ant ......
Chromosomal Location	15q25
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting MFGE8 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse MFGE8