TISIDB

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm Mitochondrion matrix, mitochondrion nucleoid Nucleus
Domain	PF02245 Methylpurine-DNA glycosylase (MPG)
Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.

> Gene Ontology [ TOP ]
Biological Process	GO:0006284 base-excision repair GO:0006285 base-excision repair, AP site formation GO:0006304 DNA modification GO:0006307 DNA dealkylation involved in DNA repair GO:0035510 DNA dealkylation GO:0045007 depurination
Molecular Function	GO:0003684 damaged DNA binding GO:0003905 alkylbase DNA N-glycosylase activity GO:0008725 DNA-3-methyladenine glycosylase activity GO:0016798 hydrolase activity, acting on glycosyl bonds GO:0016799 hydrolase activity, hydrolyzing N-glycosyl compounds GO:0019104 DNA N-glycosylase activity GO:0043733 DNA-3-methylbase glycosylase activity GO:0043916 DNA-7-methylguanine glycosylase activity GO:0052821 DNA-7-methyladenine glycosylase activity GO:0052822 DNA-3-methylguanine glycosylase activity
Cellular Component	GO:0005759 mitochondrial matrix GO:0009295 nucleoid GO:0042645 mitochondrial nucleoid

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa03410 Base excision repair
Reactome	R-HSA-73884: Base Excision Repair R-HSA-73929: Base-Excision Repair, AP Site Formation R-HSA-110331: Cleavage of the damaged purine R-HSA-73894: DNA Repair R-HSA-73927: Depurination R-HSA-110357: Displacement of DNA glycosylase by APEX1 R-HSA-110330: Recognition and association of DNA glycosylase with site containing an affected purine R-HSA-73933: Resolution of Abasic Sites (AP sites)

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between MPG and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

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Statistical results of MPG in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	Second most enriched score: 0.6	Sensitive to T cell-mediated killing
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of MPG in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	-0.305	0.306
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.444	0.883
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.2	0.927
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	-0.032	0.925
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.014	0.994
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	-0.09	0.968
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	-0.199	0.612
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	-0.572	0.654
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.127	0.933
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	-0.345	0.825
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	-0.486	0.842
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	-0.091	0.251

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of MPG in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	3.7	3.7	0.27
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	3.7	3.7	0.314
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	0	0	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	0	0	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	0	0	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	9.1	9.1	0.458
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	16.7	16.7	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	1

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of MPG. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of MPG. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by MPG. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of MPG. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of MPG expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between MPG and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	MPG
Name	N-methylpurine DNA glycosylase
Aliases	MDG; alkyladenine DNA glycosylase; AAG; ADPG; APNG; CRA36.1; PIG16; anpg; 3' end of the Mid1 gene, localized ......
Chromosomal Location	16p13.3
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting MPG collected from DrugBank database.

> Drugs from DrugBank database

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Details on drugs targeting MPG.

ID	Name	Drug Type	Targets	#Targets
DB00515	Cisplatin	Small Molecule	A2M, ATOX1, MPG, TF	4
DB01593	Zinc	Small Molecule	A1BG, A2M, AGT, AHSG, ALDOA, APCS, APLP1, APLP2, APOA1, APOA2, APO ......	119

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