TISIDB

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Domain	PF00583 Acetyltransferase (GNAT) family
Function	Acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids (PubMed:20392701). This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins. Thereby, may regulate apoptosis through the acetylation and the regulation of the expression of PROM1 (PubMed:24556617). May also regulate amyloid beta-peptide secretion through acetylation of BACE1 and the regulation of its expression in neurons (PubMed:19011241).

> Gene Ontology [ TOP ]
Biological Process	GO:0006473 protein acetylation GO:0006475 internal protein amino acid acetylation GO:0006575 cellular modified amino acid metabolic process GO:0006749 glutathione metabolic process GO:0006790 sulfur compound metabolic process GO:0018003 peptidyl-lysine N6-acetylation GO:0018205 peptidyl-lysine modification GO:0018393 internal peptidyl-lysine acetylation GO:0018394 peptidyl-lysine acetylation GO:0042493 response to drug GO:0043543 protein acylation GO:0050435 beta-amyloid metabolic process
Molecular Function	GO:0004468 lysine N-acetyltransferase activity, acting on acetyl phosphate as donor GO:0008080 N-acetyltransferase activity GO:0016407 acetyltransferase activity GO:0016410 N-acyltransferase activity GO:0016746 transferase activity, transferring acyl groups GO:0016747 transferase activity, transferring acyl groups other than amino-acyl groups GO:0047198 cysteine-S-conjugate N-acetyltransferase activity
Cellular Component	GO:0005793 endoplasmic reticulum-Golgi intermediate compartment GO:0033116 endoplasmic reticulum-Golgi intermediate compartment membrane

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa00480 Glutathione metabolism
Reactome	R-HSA-977225: Amyloid fiber formation R-HSA-392499: Metabolism of proteins

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between NAT8 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

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Statistical results of NAT8 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of NAT8 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.696	0.166
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	1.102	0.183
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	0.392	0.576
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	1.95	0.0475
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	1.236	0.4
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	2.852	0.0841
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	1.031	0.207
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	1.08	0.498
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.675	0.64
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	1.313	0.699
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	-0.38	0.937
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	-0.739	0.0134

> Mutation difference between responders and non-responders [ TOP ]
There is no record.

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of NAT8. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of NAT8. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by NAT8. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of NAT8. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of NAT8 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between NAT8 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	NAT8
Name	N-acetyltransferase 8 (GCN5-related, putative)
Aliases	Hcml1; TSC501; ATase2; N-acetyltransferase 8; CCNAT; CML1; TSC510; N-acetyltransferase 8 (camello like); ace ......
Chromosomal Location	2p13.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting NAT8 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse NAT8