TISIDB

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Nucleus outer membrane Single-pass type IV membrane protein Cytoplasmic side Sarcoplasmic reticulum membrane Single-pass type IV membrane protein Cell membrane Single-pass membrane protein Cytoplasm, cytoskeleton. Mitochondrion. Nucleus, nucleoplasm. Cytoplasm, myofibril, sarcomere, Z line Note=Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane-associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Shorter solubles isoforms can be found in the cytoplasm and within the nucleus.; SUBCELLULAR LOCATION: Isoform 8: Cell junction, focal adhesion Note=In U2OS cells.
Domain	PF00307 Calponin homology (CH) domain PF10541 Nuclear envelope localisation domain PF00435 Spectrin repeat
Function	Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probable association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 may act redundantly. During INM at G1 phase mediates respective LINC complex association with kinesin to push the nucleus away from the centrosome. Involved in nuclear migration in retinal photoreceptor progenitors. Required for centrosome migration to the apical cell surface during early ciliogenesis.

> Gene Ontology [ TOP ]
Biological Process	GO:0001667 ameboidal-type cell migration GO:0006997 nucleus organization GO:0006998 nuclear envelope organization GO:0007097 nuclear migration GO:0007163 establishment or maintenance of cell polarity GO:0010761 fibroblast migration GO:0030048 actin filament-based movement GO:0030335 positive regulation of cell migration GO:0030705 cytoskeleton-dependent intracellular transport GO:0031022 nuclear migration along microfilament GO:0032507 maintenance of protein location in cell GO:0034504 protein localization to nucleus GO:0040017 positive regulation of locomotion GO:0040023 establishment of nucleus localization GO:0045185 maintenance of protein location GO:0051235 maintenance of location GO:0051272 positive regulation of cellular component movement GO:0051640 organelle localization GO:0051642 centrosome localization GO:0051647 nucleus localization GO:0051651 maintenance of location in cell GO:0051656 establishment of organelle localization GO:0090286 cytoskeletal anchoring at nuclear membrane GO:0099515 actin filament-based transport GO:2000147 positive regulation of cell motility
Molecular Function	GO:0003779 actin binding GO:0051015 actin filament binding
Cellular Component	GO:0005635 nuclear envelope GO:0005640 nuclear outer membrane GO:0005924 cell-substrate adherens junction GO:0005925 focal adhesion GO:0016234 inclusion body GO:0016235 aggresome GO:0016528 sarcoplasm GO:0016529 sarcoplasmic reticulum GO:0019867 outer membrane GO:0030016 myofibril GO:0030017 sarcomere GO:0030018 Z disc GO:0030027 lamellipodium GO:0030055 cell-substrate junction GO:0030175 filopodium GO:0031252 cell leading edge GO:0031253 cell projection membrane GO:0031256 leading edge membrane GO:0031258 lamellipodium membrane GO:0031527 filopodium membrane GO:0031674 I band GO:0031965 nuclear membrane GO:0031968 organelle outer membrane GO:0033017 sarcoplasmic reticulum membrane GO:0034992 microtubule organizing center attachment site GO:0034993 LINC complex GO:0043292 contractile fiber GO:0044449 contractile fiber part GO:0045111 intermediate filament cytoskeleton GO:0098858 actin-based cell projection

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	R-HSA-1640170: Cell Cycle R-HSA-1500620: Meiosis R-HSA-1221632: Meiotic synapsis

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between SYNE2 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of SYNE2 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of SYNE2 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.452	0.222
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	0.914	0.585
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	0.115	0.925
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0.793	0.00713
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.55	0.865
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	1.105	0.793
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.483	0.199
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.723	0.683
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.165	0.936
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	0.536	0.743
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	1.309	0.573
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.076	0.428

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of SYNE2 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	5.9	-5.9	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	33.3	-33.3	0.231
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	25.9	4.1	21.8	0.00355
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	25.9	5.1	20.8	0.00931
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	19	17.6	1.4	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	12.5	0	12.5	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	23.1	27.3	-4.2	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	18.8	-18.8	0.28
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	22.2	-22.2	0.505
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	14.3	-14.3	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	7.9	7.4	0.5	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	4.5	15.4	-10.9	0.541
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	12.5	0	12.5	0.485
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	7.7	-7.7	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	8.3	-8.3	1

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of SYNE2. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of SYNE2. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by SYNE2. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of SYNE2. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of SYNE2 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between SYNE2 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	SYNE2
Name	spectrin repeat containing, nuclear envelope 2
Aliases	SYNE-2; DKFZP434H2235; Nesprin-2; NUANCE; NUA; KIAA1011; Nesp2; nuclear envelope spectrin repeat-2; nucleus ......
Chromosomal Location	14q23.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting SYNE2 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

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