TISIDB

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm. Cytoplasm, cytoskeleton Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity).
Domain	PF00241 Cofilin/tropomyosin-type actin-binding protein
Function	Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).

> Gene Ontology [ TOP ]
Biological Process	GO:0007015 actin filament organization GO:0008064 regulation of actin polymerization or depolymerization GO:0008154 actin polymerization or depolymerization GO:0010639 negative regulation of organelle organization GO:0030041 actin filament polymerization GO:0030042 actin filament depolymerization GO:0030047 actin modification GO:0030832 regulation of actin filament length GO:0030833 regulation of actin filament polymerization GO:0030834 regulation of actin filament depolymerization GO:0030835 negative regulation of actin filament depolymerization GO:0030837 negative regulation of actin filament polymerization GO:0031289 actin phosphorylation GO:0031333 negative regulation of protein complex assembly GO:0032271 regulation of protein polymerization GO:0032272 negative regulation of protein polymerization GO:0032507 maintenance of protein location in cell GO:0032535 regulation of cellular component size GO:0032956 regulation of actin cytoskeleton organization GO:0032970 regulation of actin filament-based process GO:0032984 macromolecular complex disassembly GO:0042989 sequestering of actin monomers GO:0043241 protein complex disassembly GO:0043242 negative regulation of protein complex disassembly GO:0043244 regulation of protein complex disassembly GO:0043254 regulation of protein complex assembly GO:0043538 regulation of actin phosphorylation GO:0043624 cellular protein complex disassembly GO:0045185 maintenance of protein location GO:0051016 barbed-end actin filament capping GO:0051235 maintenance of location GO:0051258 protein polymerization GO:0051261 protein depolymerization GO:0051493 regulation of cytoskeleton organization GO:0051494 negative regulation of cytoskeleton organization GO:0051651 maintenance of location in cell GO:0051693 actin filament capping GO:0090066 regulation of anatomical structure size GO:1901879 regulation of protein depolymerization GO:1901880 negative regulation of protein depolymerization
Molecular Function	GO:0003779 actin binding GO:0003785 actin monomer binding GO:0005543 phospholipid binding GO:0005546 phosphatidylinositol-4,5-bisphosphate binding GO:0035091 phosphatidylinositol binding GO:0045296 cadherin binding GO:0050839 cell adhesion molecule binding GO:0098631 protein binding involved in cell adhesion GO:0098632 protein binding involved in cell-cell adhesion GO:0098641 cadherin binding involved in cell-cell adhesion GO:1901981 phosphatidylinositol phosphate binding GO:1902936 phosphatidylinositol bisphosphate binding
Cellular Component	GO:0001726 ruffle GO:0005913 cell-cell adherens junction GO:0005924 cell-substrate adherens junction GO:0005925 focal adhesion GO:0015629 actin cytoskeleton GO:0030016 myofibril GO:0030055 cell-substrate junction GO:0030175 filopodium GO:0031252 cell leading edge GO:0031253 cell projection membrane GO:0031256 leading edge membrane GO:0032587 ruffle membrane GO:0043292 contractile fiber GO:0098858 actin-based cell projection

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	-

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between TWF1 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining

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Literatures describing the relation between TWF1 and anti-tumor immunity in human cancer.

PMID	Cancer type	Relation to immunity	Evidence sentences
28783722	Melanoma	Promote immunity (T cell function)	Fifteen genes showed significant resistance to T-cell-mediated cytolysis with at least 1 sgRNA in these cells.; MART-1+ Mel624 cells with sgRNAs targeting each of the 9 candidate genes showed increased resistance against these T cells (Extended Data Fig. 6b, P < 0.05). The genes that we validated across different melanoma cell lines and different antigen–TCR combinations include cellular cytoskeleton genes, COL17A1 (collagen type XVII alpha 1) and TWF1 (twinfilin-1), a microRNA (hsa-mir-101-2) and a 60S ribosomal subunit (RPL23, Supplementary Discussion).

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

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Statistical results of TWF1 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of TWF1 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.244	0.458
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.005	0.998
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	0.432	0.792
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	-0.118	0.73
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	-0.307	0.859
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0.121	0.956
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	-0.091	0.86
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.083	0.967
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	-0.289	0.897
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	-0.024	0.987
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.403	0.86
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.08	0.31

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of TWF1 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	3.7	3.7	0.27
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	3.7	3.7	0.314
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	0	0	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	0	0	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	0	0	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	1

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of TWF1. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of TWF1. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by TWF1. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of TWF1. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of TWF1 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between TWF1 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	TWF1
Name	twinfilin actin binding protein 1
Aliases	PTK9; protein tyrosine kinase 9; PTK9 protein tyrosine kinase 9; twinfilin, actin-binding protein, homolog 1 ......
Chromosomal Location	12q12
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting TWF1 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

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