TISIDB

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Late endosome membrane Peripheral membrane protein Cytoplasmic side Lysosome membrane Peripheral membrane protein Cytoplasmic side Early endosome Cytoplasmic vesicle, clathrin-coated vesicle Cytoplasmic vesicle, autophagosome Note=Colocalizes with AP-3, clathrin, Rab5 and Rab7b (By similarity). Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes.
Domain	PF04840 Vps16 PF04841 Vps16
Function	Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations (PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25266290, PubMed:25783203). Required for recruitment of VPS33A to the HOPS complex (PubMed:23901104). Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS33A but not VPS33B (PubMed:25783203). The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG (PubMed:24554770).

> Gene Ontology [ TOP ]
Biological Process	GO:0006906 vesicle fusion GO:0006914 autophagy GO:0007033 vacuole organization GO:0007034 vacuolar transport GO:0007041 lysosomal transport GO:0008333 endosome to lysosome transport GO:0016050 vesicle organization GO:0016197 endosomal transport GO:0016236 macroautophagy GO:0031338 regulation of vesicle fusion GO:0032889 regulation of vacuole fusion, non-autophagic GO:0035493 SNARE complex assembly GO:0035542 regulation of SNARE complex assembly GO:0042144 vacuole fusion, non-autophagic GO:0043254 regulation of protein complex assembly GO:0044088 regulation of vacuole organization GO:0044801 single-organism membrane fusion GO:0048284 organelle fusion GO:0060627 regulation of vesicle-mediated transport GO:0061025 membrane fusion GO:0090174 organelle membrane fusion GO:0097352 autophagosome maturation GO:0097576 vacuole fusion
Molecular Function	GO:0003779 actin binding GO:0051015 actin filament binding
Cellular Component	GO:0005765 lysosomal membrane GO:0005769 early endosome GO:0005770 late endosome GO:0005776 autophagosome GO:0010008 endosome membrane GO:0030135 coated vesicle GO:0030136 clathrin-coated vesicle GO:0030424 axon GO:0030897 HOPS complex GO:0031902 late endosome membrane GO:0043025 neuronal cell body GO:0044297 cell body GO:0044440 endosomal part GO:0055037 recycling endosome GO:0098852 lytic vacuole membrane GO:0099023 tethring complex

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	-

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between VPS16 and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of VPS16 in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	logFC: -2.14; FDR: 0.04630	Resistant to T cell-mediated killing
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of VPS16 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	-0.219	0.407
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.161	0.936
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.263	0.861
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0.066	0.809
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0.146	0.944
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	-0.032	0.991
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.129	0.662
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	0.009	0.995
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.279	0.85
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	0.277	0.848
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	0.718	0.732
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	0.019	0.756

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of VPS16 in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	7.1	0	7.1	0.452
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	25	0	25	0.222
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	7.4	4.1	3.3	0.61
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	7.4	5.1	2.3	0.647
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	9.5	0	9.5	0.492
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	12.5	0	12.5	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	7.7	0	7.7	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	11.1	6.2	4.9	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	20	0	20	0.357
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	14.3	-14.3	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	0	3.7	-3.7	0.415
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	0	0	0	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	7.1	-7.1	0.467
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	0	1

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of VPS16. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of VPS16. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by VPS16. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of VPS16. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of VPS16 expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between VPS16 and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	VPS16
Name	vacuolar protein sorting 16 homolog (S. cerevisiae)
Aliases	hVPS16; vacuolar protein sorting protein 16; Vacuolar protein sorting-associated protein 16 homolog
Chromosomal Location	20p13
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting VPS16 collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse VPS16