Cancer

acute myeloid leukemia

Regulation

deregulation

Reporting

TaqMan miRNA microarray was performed to identify deregulated miRNAs in the plasma of AML patients. Quantitative real-time RT-PCR was used to validate the results. The plasma expression level of let-7d miR-150 miR-339 and miR-342 was down-regulated whilst that of let-7b and miR-523 was up-regulated in the AML group at diagnosis compared to healthy controls. QRT-PCR results showed that the expression level of these two miRs in complete remission AML patients resembled that of healthy controls

Pubmed

23391324

Method

Microarray; qPCR

Cancer

breast cancer

Regulation

deregulation

Reporting

Breast cancer signatures for invasiveness and prognosis defined by deep sequencing of microRNA. We selected them from a recently published deep-sequencing dataset Farazi TA et al 2011 Cancer Res 71:4443-4453. Specifically let-7d miR-210 and -221 were down-regulated in the in situ and up-regulated in the invasive transition thus featuring an expression reversal along the cancer progression path

Pubmed

22315424

Method

RNA-Seq

Cancer

glioblastoma

Regulation

upregulation

Reporting

Specifically miR-181b miR-153 miR-145 miR-137 and let-7d were significantly upregulated after treatment with both TMZ and OLE

Pubmed

22722712

Method

Cancer

pancreatic cancer

Regulation

deregulation

Reporting

The primary objective of this study was to determine the feasibility of performing miRNA arrays and quantitative real-time PCR qRT-PCR from archival formalin-fixed paraffin-embedded FFPE cell blocks obtained from fine-needle aspirates FNAs that is the commonest diagnostic procedure for suspected PC. Exiqon assay was used to quantify miRNA levels and qRT-PCR was used to validate abnormal expression of selected miRNAs. Profiling demonstrated deregulation of over 228 miRNAs in pancreatic adenocarcinoma of which the top 7 were further validated by qRT-PCR. MicroRNAs let-7d and miR-423-5p was either downregulated or upregulated with a significant inter-individual variation in their expression

Pubmed

22929886

Method

qPCR; Microarray

Cancer

prostate cancer

Regulation

downregulation

Reporting

We investigated the expression profiles of 6 microRNAs let-7g let-7d miR-98 miR-96 miR-182 and miR-183 using the method of locked nucleic acid LNA-modified oligonucleotide in situ hybridization ISH and the technology of tissue microarray TMA with the formalin-fixed paraffin-embedded FFPE specimens from 52 patients with PCa and 38 with benign prostatic hyperplasia BPH. Compared with BPH the PCa patients showed decreased expressions of miR-98 let-7d and let-7g and decreased expressions of miR-96 miR-182 and miR-183 with statistically significant differences between the two groups P < 0.05. There was a positive correlation among the expressions of miR-96 miR-182 and miR-183 P = 0.00 r = 0.41 as well as between the expressions of let-7d and let-7g P = 0.00 r = 0.46 in the PCa tissues. And the expression of miR-98 was positively correlated with those of let-7d and let-7g P = 0.00 r = 0.46. The expression profiles of the microRNAs let-7d let-7g miR-98 miR-96 miR-182 and miR-183 reflect the biological behavior of PCa to some extent and might be important biomarkers for the early detection and prognostic assessment of prostate cancer

Pubmed

20873592

Method

Microarray; in situ hybridization