Cancer

breast cancer

Regulation

deregulation

Reporting

We selected 18 miRNAs with predicted roles in breast cancer and demonstrated that let-7i p = 0.019 let-7a p = 0.02 and miR-650 p = 0.008 were significantly up-regulated in plasma; miR-21 p < 0.001 is up-regulated in breast cancer tissue and miR-30e was down-regulated in both plasma p < 0.001 and breast cancer tissues p = 0.004

Pubmed

27012041

Method

Cancer

colorectal cancer

Regulation

deregulation

Reporting

Four miRNAs downregulated in LM let-7i miR-10b miR-221 and miR-320a and one upregulated miR miR-885-5p were quantitatively validated in pCRC P < .0001. Low let-7i expression in pCRC tissue predicted worsened prognosis hazard ratio HR = 5.0 95% confidence interval CI = 1.0 to 24.4 P = .0479 as well as distant metastasis odds ratio OR = 5.5 95% CI = 1.1 to 26.8 P = .0334. Expression patterns of miRNAs were confirmed by in situ hybridization

Pubmed

25663689

Method

in situ hybridization

Cancer

gastric cancer

Regulation

downregulation

Reporting

Decreased expression of microRNA let 7i and its association with chemotherapeutic response in human gastric cancer. MicroRNA let-7i has been proven to be down-regulated in many human malignancies and correlated with tumor progression and anticancer drug resistance. Our study aims to characterize the contribution of miRNA let-7i to the initiation and malignant progression of locally advanced gastric cancer LAGC and evaluate its possible value in neoadjuvant chemotherapeutic efficacy prediction. Let-7i expression was examined for pairs of cancer tissues and corresponding normal adjacent tissues NATs using quantitative RT-PCR. The relationship of let-7i level to clinicopathological characteristics pathologic tumor regression grades after chemotherapy and overall survival OS was also investigated. Let-7i was significantly down-regulated in most tumor tissues 78/86: 91% compared with paired NATs P < 0.001 and low levels of let-7i were significantly correlated with local invasion lymphatic metastasis and poor pathologic tumor response. Multivariate Cox regression analysis revealed that low let-7i expression was an unfavorable prognostic factor of OS hazard ratio HR = 2.316 P = 0.024 independently of other clinicopathological factors including tumor node metastasis TNM stage HR = 3.226 P = 0.013 depth of infiltration HR = 4.167 P < 0.001 and lymph node status HR = 2.245 P = 0.037. These findings indicate that let-7i may be a good candidate for use a therapeutic target and a potential tissue marker for the prediction of chemotherapeutic sensitivity and prognosis in LAGC patients

Pubmed

23107361

Method

qPCR

Cancer

glioblastoma

Regulation

downregulation

Reporting

Here miRNA expression profiles from 82 pGBM samples were analyzed and 78 independent pGBM samples were used for qRT-PCR validation. A classifier consisting of seven miRNAs was then identified miR-1280 miR-1238 miR-938 and miR-423-5p overexpressed in the TCR subtype; and let-7i miR-151-3p and miR-93 downregulated in the TCR subtype which could be used to assign pGBM samples to the corresponding subtype

Pubmed

25869098

Method

qPCR

Cancer

lung cancer

Regulation

downregulation

Reporting

In this study we determined the serum miRNA profiles of 10 nonsmokers 10 smokers and 10 lung-cancer patients with miRCURY LNA microRNA arrays. We found that let-7i-3p and miR-154-5p were significantly downregulated in the sera of smokers and lung-cancer patients so the serum levels of let-7i-3p and miR-154-5p are associated with smoking and smoking-related lung cancer. The areas under receiver operating characteristic curves for let-7i-3p and miR-154-5p were approximately 0.892 and 0.957 respectively. In conclusion our results indicate that changes in serum miRNAs are associated with cigarette smoking and lung cancer and that let-7i-3p and miR-154-5p are potential biomarkers of smoking-related lung cancer

Pubmed

25386559

Method

Microarray