Cancer

breast cancer

Regulation

upregulation

Reporting

Then five up-regulated miRNAs miR-100 miR-29a miR-196a miR-222 and miR-30a in both MCF-7/Adr and MCF-7/Doc were selected to explore their roles in acquisition of drug-resistance using transfection experiment

Pubmed

23994196

Method

Cancer

cervical and endocervical cancer

Regulation

upregulation

Reporting

The expression of miR-196a in cervical cancer cell lines and cervical cancer tissues was examined using real-time PCR. The effects of miR-196a on PI3K/Akt signalling and cellular proliferation were evaluated by bromodeoxyuridine labelling 3-45-Dimethyl-2-thiazolyl-25-diphenyl-2H-tetrazoliumbromide colony formation assays and luciferase assays. The expression level of miR-196a was markedly increased in cervical cancer tissues and cell lines compared with normal cervical tissue and normal cervical squamous cells. Upregulation of miR-196a was correlated with advanced tumour stage and poor overall and recurrence-free survival in cervical cancer patients. Our findings suggest that miR-196a has an important role in promoting human cervical cancer cell proliferation and may represent a novel therapeutic target of microRNA-mediated suppression of cell proliferation in cervical cancer

Pubmed

24423924

Method

qPCR

Cancer

cervical and endocervical cancer

Regulation

upregulation

Reporting

Clinical significance of serum miR 196a in cervical intraepithelial neoplasia and cervical cancer. Previous studies have reported that miR-196a is upregulated in cervical cancer tissues and cell lines. Quantitative reverse transcription-polymerase chain reaction was performed to compare the serum levels miR-196a in all participants. Cervical cancer patients with higher serum miR-196a levels had a poorer overall survival rate P = 0.004. Multivariate analysis revealed that high serum miR-196a was an independent predictor for poor survival of cervical cancer HR = 3.510; 95%CI = 1.961-6.874; P = 0.025. Therefore serum miR-196a may be a reliable biomarker for early detection and prognosis of cervical cancer

Pubmed

26782446

Method

Reverse transcription PCR

Cancer

colon cancer

Regulation

upregulation

Reporting

To present proof-of-principle application for employing micromiRNAs as diagnostic markers for colon cancer we carried out global microarray expression studies on stool samples obtained from fifteen individuals three controls and three each with TNM stage 0-1 stage 2 stage 3 and stage 4 colon cancer using Affymetrix GeneChip miRNA 3.0 Array to select for a panel of miRNA genes for subsequent focused semi-quantitative polymerase chain reaction PCR analysis studies. Microarray results showed 202 preferentially expressed miRNA genes that were either increased 141 miRNAs or reduced 61 miRNAs in expression. Results of the PCR analyses confirmed that twelve miRNAs miR-7 miR-17 miR-20a miR-21 miR-92a miR-96 miR-106a miR-134 miR-183 miR-196a miR-199a-3p and miR214 had an increased expression in the stool of patients with colon cancer and that later TNM carcinoma stages exhibited a more pronounced expression than did adenomas

Pubmed

23741026

Method

qPCR; Microarray

Cancer

colorectal cancer

Regulation

upregulation

Reporting

Upregulation of microRNA 196a and microRNA 196b cooperatively correlate with aggressive progression and unfavorable prognosis in patients with colorectal cancer. Both microRNA miR-196a and miR-196b are implicated in normal cell differentiation proliferation and in tumorigenesis of various cancer types. Especially miR-196a exerts a pro-oncogenic influence in colorectal cancer CRC cells and miR-196b expression is upregulated in CRC tissues. The aim of this study was to evaluate the associations of miR-196a and miR-196b dysregulation with clinicopathological characteristics and prognosis in patients with CRC. Quantitative real time-PCR qRT-PCR was performed to detect the expression levels of miR-196a and miR-196b in 126 pairs of fresh tumor samples matched with adjacent colorectal mucosa obtained from 126 patients with CRC miR-196a and miR-196b expression levels in CRC tissues were significantly higher than those in adjacent colorectal mucosa both P < 0.002. Interestingly the expression levels of miR-196a in CRC tissues were positively correlated with those of miR-196b. Then high miR-196a expression and high miR-196b expression alone or in combination were all statistically linked to the presence of lymph node metastasis the poor differentiation grade and the advanced TNM stage of CRC. Moreover overall and disease-free survivals of CRC patients with high miR-196a expression high miR-196b expression and miR-196a-high/miR-196b-high expression tended to be shorter than the corresponding control groups log-rank statistic all P < 0.001. Furthermore multivariate analysis indicated miR-196a and/or miR-196b expression as independent prognostic indicators for CRC patients all P < 0.05. Both miR-196a and miR-196b may be correlated with aggressive progression and unfavorable clinical outcome in CRC patients. Combined expression of miR-196a and miR-196b may be a promising biomarker in identifying a poor prognosis group of CRC

Pubmed

25525411

Method

qPCR

Cancer

gastric cancer

Regulation

upregulation

Reporting

In the analysis by real-time PCR-based miRNA arrays using pooled RNA samples from five gastric cancer patients expression of miR-107 miR-21 miR-196a miR-26b miR-9 miR-142-3p miR-30b miR-150 miR-191 and miR-17 was found to be upregulation

Pubmed

22407237

Method

qPCR; Microarray

Cancer

gastric cancer

Regulation

upregulation

Reporting

Association of miR 193b down regulation and miR 196a up regulation with clinicopathological features and prognosis in gastric cancer. To investigate the hypothesis that genome-wide alternations of miRNAs differentiate gastric cancer tissues from those matched adjacent non-tumor tissues ANTTs miRNA arrays were employed to examine miRNA expression profiles for the 5-pair discovery stage and the quantitative real-time polymerase chain reaction qRT- PCR was applied to validate candidate miRNAs for 48-pair validation stage. qRT-PCR further confirmed the variation in expression of miR-193b and miR-196a in the validation stage. Down-expression of miR-193b was significantly correlated with Lauren type differentiation UICC stage invasion and metastasis of gastric cancer p<0.05 while over-expression of miR-196a was significantly associated with poor differentiation p=0.022. Overall survival time of patients with a low fold-change of up-regulated miR- 196a n=27; median survival=52 months was significantly longer than that of patients with a high fold-change of up-regulated miR-196a n=21; median survival=46 months p=0.003. Hence miR-193b and miR-196a may be applied as novel and promising prognostic markers in gastric cancer

Pubmed

25374225

Method

qPCR; Microarray

Cancer

gastric cancer

Regulation

upregulation

Reporting

Clinical significance of upregulation of mir 196a 5p in gastric cancer and enriched KEGG pathway analysis of target genes. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer. The expression of mir-196a-5p in poorly moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT- qPCR. Subsequently an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues 47/58. Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage P <0.05; x2 test. Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology. Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis suggesting roles as an oncogenic miRNA biomarker in gastric cancer

Pubmed

25773825

Method

qPCR

Cancer

gastric cancer

Regulation

upregulation

Reporting

miR-196a and/or miR-196b involved in cancer initiation and progression are frequently upregulated in tumour tissues. The quantitative real time-polymerase chain reaction data revealed markedly higher pre-operative circulating miR-196a and miR-196b levels in GC patients than healthy controls. Circulating miR-196a exhibited higher diagnostic capacity than combined miR-196a/b or miR-196b alone highlighting its potential as an effective plasma biomarker for GC

Pubmed

27420607

Method

qPCR

Cancer

glioblastoma

Regulation

upregulation

Reporting

Recent studies have revealed that miR-196a is upregulated in glioblastoma multiforme GBM and that it correlates with the clinical outcome of patients with GBM. We used quantitative real-time PCR to assess miR-196a expression levels in 132 GBM specimens in a single institution. Immunohistochemistry was used to determine the expression of IκBα in GBM tissues and a luciferase reporter assay was carried out to confirm whether IκBα is a direct target of miR-196a. In vivo xenograft tumors were examined for an antiglioma effect of miR-196a inhibitors. Our study confirmed that miR-196a was upregulated in GBM specimens and that high levels of miR-196a were significantly correlated with poor outcome in a large cohort of GBM patients. Our data from human tumor xenografts in nude mice treated with miR-196 inhibitors demonstrated that inhibition of miR-196a could ameliorate tumor growth in vivo. Our findings provide new insights into the pathogenesis of GBM and indicate that miR-196a may predict clinical outcome of GBM patients and serve as a new therapeutic target for GBM

Pubmed

24463357

Method

qPCR

Cancer

head and neck cancer

Regulation

upregulation

Reporting

MicroRNA miRNA expression profiling of a panel of HNSCC tumours with and without recurrent disease after surgery and radiotherapy detected miR-196a as one of the highest upregulated miRNAs in the poor prognostic group. In conclusion this study identifies miR-196a as a potential important biomarker of prognosis and response of HNSCC to radiotherapy

Pubmed

25523631

Method

Cancer

kidney renal cell cancer

Regulation

downregulation

Reporting

Meanwhile the rs11614913 CC genotype was associated with the significantly decreased expression of miR-196a-5p in 26 renal cancer tissues P = 0.018

Pubmed

24681820

Method

Cancer

liver cancer

Regulation

upregulation

Reporting

Compared with the matched NT tissues 9 miRNAs were up-regulated in the HCC tissues and three were considered statistically significant p < 0.05: miR-96 miR-183 and miR-196a which were up-regulated 4.746- 7.127- and 3.498-fold respectively

Pubmed

24273923

Method

Cancer

melanoma

Regulation

downregulation

Reporting

Resulting from a screening for microRNAs differentially regulated in melanocytes and melanoma cells we found expression of miR-196a to be significantly down-regulated in malignant melanoma cell lines and tissue samples. As it was stated before that miR-196a might negatively regulate expression of the transcription factor HOX-C8 we analyzed HOX-C8 levels in NHEMs and melanoma cells and found a strong up-regulation of HOX-C8 expression in malignant melanoma cell lines and tissue samples compared with melanocytes. Luciferase assays using reporter plasmids containing different fragments of the HOX-C8 3'UTR confirmed direct interactions of miR-196a with the HOX-C8 mRNA

Pubmed

21077158

Method

Cancer

ovarian cancer

Regulation

upregulation

Reporting

Increased expression of microRNA 196a predicts poor prognosis in human ovarian carcinoma. Overexpression of MicroRNA-196a miR-196a has recently been reported in different types of human cancers. However the prognostic value of miR-196a in ovarian carcinoma remains unknown. In this study we investigated the expression of miR-196a in ovarian carcinoma and its relationship with tumor progression and clinical prognosis. The expression level of miR-196a was examined by quantitative Real-time PCR qRT-PCR in surgically removed ovarian cancer tissues and ovarian cancer cell lines. The correlation between miR-196a expression and clinical features and prognosis were statistically analyzed. The results showed that the miR-196a expression was significantly upregulated in tumor tissues and ovarian cancer cell lines compared with that in normal ovarian surface tissues and normal ovarian epithelial cells. Moreover miR-196a expression was positively correlated with FIGO stage P<0.001 tumor size P=0.020 and lymph nodes metastasis P=0.019. Kaplan-Meier analysis demonstrated that high levels of miR-196a expression was associated with poorer overall survival P<0.001 and recurrent-free survival P=0.003 especially in patients with advanced disease P=0.002. Multivariate analysis suggested that miR-196a expression was an independent prognostic factor for overall survival of patients with ovarian carcinoma. In conclusion miR-196a may play an important role in the progression of ovarian carcinoma and could be used as an independent prognostic biomarker for patients with ovarian carcinoma

Pubmed

26097603

Method

qPCR

Cancer

pancreatic cancer

Regulation

upregulation

Reporting

Profiling of 95 microRNAs in pancreatic cancer cell lines and surgical specimens by real time PCR analysis. Differential expression of 95 miRNAs was analyzed by real time RT-PCR using the QuantiMir System. Eight miRNAs were significantly upregulated in most pancreatic cancer tissues and cell lines including miR-196a miR-190 miR-186 miR-221 miR-222 miR-200b miR-15b and miR-95

Pubmed

19030927

Method

qPCR

Cancer

pancreatic cancer

Regulation

downregulation

Reporting

Four miRNAs miR-17-5p miR-21 miR-155 and miR-196a related to PC were selected for examination in our research. There were low expressions of exosomal miR-155 and miR-196a in serum samples of PC patients when U-6 was used as a control

Pubmed

24007214

Method

Cancer

sarcoma

Regulation

upregulation

Reporting

miR 196a expression in human and canine osteosarcomas: a comparative study. Here we compared the effects of miR-196a deregulation in human and canine OS cells after having observed a more uniform distribution and stronger down-expression in the human specimens. Cell response to miR-196a transfection was different in human and canine OS. Transient decrease of motility was highly remarkable and longer in MG63 concomitant with decreased levels of annexin1 a target of miR-196a promoting cell migration and invasion. In conclusion the effects of miR-196a over-expression on tumour cell response may be strictly related to species and cell type

Pubmed

25599934

Method