Cancer

gastric cancer

Regulation

downregulation

Reporting

miR-485-5p is reported as a potential suppressor in breast cancer but its expression cellular function and clinic features in gastric cancer is not known. We also found that miR-485-5p suppressed gastric cancer cell metastasis and sphere formation. The low expression of miR-485-5p in gastric cancer tissues was related to advanced clinical features and poorer prognosis

Pubmed

26807169

Method

Cancer

gastric cancer

Regulation

downregulation

Reporting

Reduced miR 485 5p expression predicts poor prognosis in patients with gastric cancer. The present study was designed to explore expression and prognostic value of miR-485-5p in patients with gastric cancer. We determined the expression level of miR-485-5p in 132 cases of paired GC and adjacent non-tumor tissues by quantitative real-time PCR qRT-PCR. The relevance of miR-485-5p expression to the clinicopathological factors was assessed. The expression of miR-485-5p was significantly down-regulated in GC tissues compared with adja-cent normal tissues p < 0.01. MiR-485-5p expression was positively correlated with larger tumor size p = 0.003 deeper invasion depth p = 0.005 positive lymph node metastasis p = 0.039 advanced tumor-node-metastasis TNM stage p = 0.017. Patients survival analysis showed that a clear positive correlation between miR-485-5p expression level and survival time of gastric cancer patients p < 0.001. Multivariate analyses confirmed that a low level of miR-485-5p expression was an independent predictor of poor prognosis in GC patients. Expression level of miR-485-5p serves as a novel biomarker for the overall survival of patients with gastric cancer

Pubmed

27160123

Method

qPCR

Cancer

liver cancer

Regulation

downregulation

Reporting

We found that miR-485-5p was significantly downregulated in HCC tissues and that its expression was inversely correlated with the TNM stage and metastasis in HCC samples. Results of cellular functions in HCC showed that miR-485-5p could inhibit cell proliferation and metastasis

Pubmed

26054676

Method

Cancer

lung cancer

Regulation

downregulation

Reporting

In this study we found that the expression level of miR-485 was downregulated in four lung adenocarcinoma cell lines and tissues and that the reduced miR-485 expression was associated with tumor metastasis. In conclusion the present study provided novel insight into the molecular mechanism of lung adenocarcinoma progression and demonstrating miR-485 as a potential therapeutic target in human lung adenocarcinoma

Pubmed

27262438

Method

Cancer

sarcoma

Regulation

deregulation

Reporting

Hypoxic conditions were verified and miRNA expression profiles were assessed by LNA™ oligonucleotide microarrays and RT-PCR after 24 h. The expression of target genes regulated by hypoxia responsive miRNAs is examined by end-point PCR and validated by luciferase reporter constructs. The most differentially expressed miRNAs p<0.001 in response to hypoxia were miR-185-3p miR-485-5p miR-216a-5p upregulated and miR-625-5p downregulated. Target prediction algorithms identified 11 potential binding sites for miR-485-5p and a single putative miR-210-3p binding site in the 3'UTR of HIF3α the least studied member of the HIF family. Additionally luciferase reporter constructs containing the 3'UTR of HIF3α were used to demonstrate regulation of HIF3α by miR-210-3p and miR-485-5p

Pubmed

24927770

Method

Microarray