Cancer

breast cancer

Regulation

downregulation

Reporting

Systematic analysis of gene expression pattern in has miR 760 overexpressed resistance of the MCF 7 human breast cancer cell to doxorubicin. miR-760 was significantly downregulated in chemoresistance breast cancer tissues compared to chemo-sensitive tissues in our previous study. However the role of miR-760 in modulating drug resistance remains largely unexplored. Our study explored the genes expression pattern after miR-760 overexpresssed and confirmed 3 dominantly dysregulated genes which could expand the insights into the miR-760 function and molecular mechanisms in drug resistance of breast cancer. This study might afford a comprehensive understanding of miR-760 as prognostic biomarkers during clinical treatment and we supposed that the miR-760 expression levels in drug resistance carcinoma tissues could be pursued to develop new strategies for targeted therapies in chemoresistant breast cancer patients

Pubmed

25661353

Method

Cancer

gastric cancer

Regulation

downregulation

Reporting

Contrasting expression patterns of histone mRNA and microRNA 760 in patients with gastric cancer. Here RNA-seq analysis of bone marrow from gastric cancer patients was performed to identify prognostic markers for gastric cancer. Bone marrow samples from eight gastric cancer patients stages I and IV: n = 4 each were used for RNA-seq analysis. miR-760 expression was assayed using qRT-PCR in 105 bone marrow and 96 primary tumor samples. Luciferase reporter assays were performed to confirm whether histone mRNAs were direct targets of miR-760 miR-760 expression was also evaluated in noncancerous cells from gastric cancer patients. RNA-seq analysis of bone marrow samples from gastric cancer patients revealed higher expression of multiple histone mRNAs in stage IV patients. In contrast miR-760 was downregulated in the bone marrow and primary tumor of stage IV patients compared with stage I patients P = 0.0094 and 0.0018 respectively. Histone mRNA and miR-760 interacted directly. Furthermore miR-760 was downregulated in noncancerous mucosa in stage IV gastric cancer patients. Histone mRNA was upregulated whereas miR-760 was downregulated in the bone marrow and primary tumor of advanced gastric cancer patients suggesting that the histone mRNA/miR-760 axis had a crucial role in the development of gastric cancer

Pubmed

24097871

Method

RNA-Seq; qPCR

Cancer

ovarian cancer

Regulation

upregulation

Reporting

miR-760 expression in ovarian cancer cell lines and patient tissues were determined using Real-time PCR. Functional assays such as MTT anchorage-independent growth colony formation and BRDU assay were used to determine the oncogenic role of miR-760 in human ovarian cancer progression. The expression of miR-760 was markedly upregulated in ovarian cancer cell lines and tissues and high miR-760 expression was associated with an aggressive phenotype and poor prognosis with ovarian cancer patients. Our findings suggest that miR-760 represents a potential onco-miR and participates in ovarian cancer carcinogenesis which highlight its potential as a target for ovarian cancer therapy

Pubmed

27726922

Method

qPCR