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This regulatory network was inferred from the input dataset. The miRNAs and mRNAs are presented as round and rectangle nodes respectively. The numerical value popped up upon mouse over the gene node is the log2 transformed fold-change of the gene expression between the two groups. All of the nodes are clickable, and the detailed information of the miRNAs/mRNAs and related cancer pathway will be displayed in another window. The edges between nodes are supported by both interactions (predicted or experimentally verified) and correlations learnt from cancer dataset. The numerical value popped up upon mouse over the edge is the correlation beat value (effect size) between the two nodes. The experimental evidences of the edges reported in previous cancer studies are highlighted by red/orange color. All of these information can be accessed by the "mouse-over" action. This network shows a full map of the miRNA-mRNA regulation of the input gene list(s), and the hub miRNAs (with the high network degree/betweenness centrality) would be the potential cancer drivers or tumor suppressors. The full result table can be accessed in the "Regulations" tab.

"miRNACancerMAP" is also a network visualization tool for users to draw their regulatory network by personal customization. Users can set the complexity of the network by limiting the number of nodes or edges. And the color of the nodes can be defined by different categories of the mRNAs and miRNAs, such as Gene-Ontology, pathway, and expression status. Users can also select to use network degree or network betweenness centrality to define the node size. And edges can be black or colored by the correlation. Purple edge means negative correlation (mostly found between miRNA and mRNA), and blue edge means positive correlation (found in PPI or miRNA-miRNA sponge effect). We can also add the protein-protein interactions (PPI) into the network. This result will show the cluster of genes regulated by some specific miRNAs. Additionally, miRNA-miRNA edges can be added by the "miRNA sponge" button, presenting some clusters of miRNAs that have the interactions via sponge effect.

miRNA-gene regulations

(Download full result)

Num microRNA           Gene miRNA log2FC miRNA pvalue Gene log2FC Gene pvalue Interaction Correlation beta Correlation P-value PMID Reported in cancer studies
1 hsa-miR-140-5p ABCD3 -0.76 0 0.36 0.0002 miRanda -0.18 0 NA
2 hsa-miR-152-3p ABCD3 -0.27 0.00639 0.36 0.0002 MirTarget -0.15 1.0E-5 NA
3 hsa-miR-140-5p ABHD1 -0.76 0 -0.98 0 miRanda -0.13 0.01184 NA
4 hsa-miR-34a-5p ABHD2 0.13 0.13326 0.45 0.00053 mirMAP -0.12 0.01835 NA
5 hsa-miR-194-5p ACADSB 0.47 0 -0.52 0.002 mirMAP -0.23 0.00038 NA
6 hsa-miR-34a-5p ACSL4 0.13 0.13326 -1.48 0 MirTarget; miRNATAP -0.11 0.02677 NA
7 hsa-miR-34a-5p ADAM22 0.13 0.13326 -1.22 0 MirTarget; miRNATAP -0.33 5.0E-5 NA
8 hsa-miR-140-5p ADAMTS13 -0.76 0 0.49 0.00058 mirMAP -0.38 0 NA
9 hsa-miR-152-3p ADAMTS19 -0.27 0.00639 0.71 0.02881 MirTarget -0.36 0.00184 NA
10 hsa-miR-194-5p ADAMTS5 0.47 0 -3.55 0 mirMAP -0.42 0 NA
11 hsa-miR-140-5p ADCY1 -0.76 0 -0.05 0.84149 mirMAP -0.43 0 NA
12 hsa-miR-34a-5p ADCY1 0.13 0.13326 -0.05 0.84149 mirMAP -0.24 0.01304 NA
13 hsa-miR-140-5p ADCY6 -0.76 0 -0.63 0 MirTarget; PITA; miRanda; miRNATAP -0.16 0 NA
14 hsa-miR-194-5p AFF2 0.47 0 -1.78 0 mirMAP -0.18 0.01098 NA
15 hsa-miR-34a-5p AKAP13 0.13 0.13326 -0.61 0 mirMAP -0.11 0.0002 NA
16 hsa-miR-34a-5p AKAP6 0.13 0.13326 -2.28 0 MirTarget -0.13 0.0257 NA
17 hsa-miR-194-5p ALDH1L2 0.47 0 -0.04 0.78616 mirMAP -0.2 0.00037 NA
18 hsa-miR-140-5p ALOX15B -0.76 0 -0.75 0.01908 miRanda -0.25 0.0205 NA
19 hsa-miR-34a-5p ALX4 0.13 0.13326 -3.22 0 mirMAP -0.47 2.0E-5 NA
20 hsa-miR-140-5p AMACR -0.76 0 0.53 0 miRanda -0.15 0 NA
21 hsa-miR-194-5p ANGPTL1 0.47 0 -3.72 0 MirTarget -0.55 0 NA
22 hsa-miR-34a-5p ANK2 0.13 0.13326 -2.51 0 MirTarget; miRNATAP -0.32 0 NA
23 hsa-miR-34a-5p ANK3 0.13 0.13326 -0.03 0.84332 miRNATAP -0.14 0.00547 NA
24 hsa-miR-140-5p ANKRD42 -0.76 0 0.22 0.01213 MirTarget; miRanda -0.21 0 NA
25 hsa-miR-140-5p ANKRD52 -0.76 0 0.23 0.00024 PITA; miRanda; miRNATAP -0.12 0 NA
26 hsa-miR-34a-5p ANKS1A 0.13 0.13326 -0.37 0 MirTarget -0.1 2.0E-5 NA
27 hsa-miR-140-5p ANKS1B -0.76 0 0.2 0.2194 miRanda -0.32 0 NA
28 hsa-miR-140-5p AP1AR -0.76 0 0.37 0 miRanda -0.11 2.0E-5 NA
29 hsa-miR-140-5p AP2B1 -0.76 0 0.26 0.00183 PITA; miRanda -0.13 0 NA
30 hsa-miR-140-5p ARFIP2 -0.76 0 0.83 0 PITA; miRanda -0.3 0 NA
31 hsa-miR-34a-5p ARHGAP19 0.13 0.13326 -0.97 0 mirMAP -0.11 0.00019 NA
32 hsa-miR-194-5p ARHGAP24 0.47 0 -1.41 0 miRNATAP -0.23 0 NA
33 hsa-miR-140-5p ARHGAP32 -0.76 0 0.43 9.0E-5 miRanda -0.19 0 NA
34 hsa-miR-194-5p ARHGAP5 0.47 0 -0.91 0 miRNATAP -0.18 0 NA
35 hsa-miR-140-5p ARHGEF18 -0.76 0 0.6 0 miRanda -0.11 0 NA
36 hsa-miR-34a-5p ARHGEF33 0.13 0.13326 -0.66 0 MirTarget -0.1 0.03642 NA
37 hsa-miR-194-5p ARID4A 0.47 0 -0.86 0 miRNATAP -0.14 1.0E-5 NA
38 hsa-miR-140-5p ARMC9 -0.76 0 1.05 0 miRanda -0.26 0 NA
39 hsa-miR-140-5p ARV1 -0.76 0 0.72 0 miRanda -0.13 0 NA
40 hsa-miR-34a-5p ASTN1 0.13 0.13326 -2.96 0 MirTarget -0.54 0 NA
41 hsa-miR-34a-5p ASXL2 0.13 0.13326 -0.86 0 MirTarget -0.17 0.00682 NA
42 hsa-miR-140-5p ATG4B -0.76 0 0.43 0 miRanda -0.11 0 NA
43 hsa-miR-140-5p ATP6V1G1 -0.76 0 0.81 0 miRanda -0.17 0 NA
44 hsa-miR-194-5p ATP8B4 0.47 0 -1.46 0 MirTarget -0.25 0 NA
45 hsa-miR-140-5p B3GALT6 -0.76 0 0.69 0 miRanda -0.16 0 NA
46 hsa-miR-140-5p BBC3 -0.76 0 1.65 0 miRNATAP -0.47 0 NA
47 hsa-miR-140-5p BBS4 -0.76 0 0.09 0.27948 miRanda -0.15 0 NA
48 hsa-miR-34a-5p BCL2 0.13 0.13326 -0.9 0 miRTarBase -0.22 0.00143 24565525; 22623155; 21399894; 23155233; 24444609; 20687223; 24988056; 18803879; 19714243; 25053345; 20433755; 22964582; 23862748 In vitro and in vivo experiments showed that miR-34a and DOX can be efficiently encapsulated into HA-CS NPs and delivered into tumor cells or tumor tissues and enhance anti-tumor effects of DOX by suppressing the expression of non-pump resistance and anti-apoptosis proto-oncogene Bcl-2;MiR 34a inhibits proliferation and migration of breast cancer through down regulation of Bcl 2 and SIRT1; In this study we aimed to determine the effect of miR-34a on the growth of breast cancer and to investigate whether its effect is achieved by targeting Bcl-2 and SIRT1; Bcl-2 and SIRT1 as the targets of miR-34a were found to be in reverse correlation with ectopic expression of miR-34a;Quantitative PCR and western analysis confirmed decreased expression of two genes BCL-2 and CCND1 in docetaxel-resistant cells which are both targeted by miR-34a;The miR-34a expression levels in cells after irradiation at 30 and 60 Gy were 0.17- and 18.7-times the BCL2 and caspase-9 expression levels respectively;Functional analyses further indicate that restoration of miR-34a inhibits B cell lymphoma-2 Bcl-2 protein expression to withdraw the survival advantage of these resistant NSCLC cells;Thus in PC3PR cells reduced expression of miR-34a confers paclitaxel resistance via up-regulating SIRT1 and Bcl2 expression; MiR-34a and its downstream targets SIRT1 and Bcl2 play important roles in the development of paclitaxel resistance all of which can be useful biomarkers and promising therapeutic targets for the drug resistance in hormone-refractory prostate cancer;Target analysis indicated that micro RNA miR-34a directly regulates Bcl-2 and miR-34a overexpression decreased Bcl-2 protein level in gastric cancer cells; We also found that luteolin upregulates miR-34a expression and downregulates Bcl-2 expression; Based on these results we can draw the conclusion that luteolin partly decreases Bcl-2 expression through upregulating miR-34a expression;miR-34 targets Notch HMGA2 and Bcl-2 genes involved in the self-renewal and survival of cancer stem cells; Human gastric cancer cells were transfected with miR-34 mimics or infected with the lentiviral miR-34-MIF expression system and validated by miR-34 reporter assay using Bcl-2 3'UTR reporter; Human gastric cancer Kato III cells with miR-34 restoration reduced the expression of target genes Bcl-2 Notch and HMGA2; Bcl-2 3'UTR reporter assay showed that the transfected miR-34s were functional and confirmed that Bcl-2 is a direct target of miR-34; The mechanism of miR-34-mediated suppression of self-renewal appears to be related to the direct modulation of downstream targets Bcl-2 Notch and HMGA2 indicating that miR-34 may be involved in gastric cancer stem cell self-renewal/differentiation decision-making;Among the target proteins regulated by miR-34 are Notch pathway proteins and Bcl-2 suggesting the possibility of a role for miR-34 in the maintenance and survival of cancer stem cells; Our data support the view that miR-34 may be involved in pancreatic cancer stem cell self-renewal potentially via the direct modulation of downstream targets Bcl-2 and Notch implying that miR-34 may play an important role in pancreatic cancer stem cell self-renewal and/or cell fate determination;Manipulating miR-34a in prostate cancer cells confirms that this miRNA regulates BCL-2 and may in part regulate response to docetaxel;For instance miR-34a up-regulation corresponded with a down-regulation of BCL2 protein; Treating Par-4-overexpressing HT29 cells with a miR-34a antagomir functionally reversed both BCL2 down-regulation and apoptosis by 5-FU;Tumors harvested from these lungs have elevated levels of oncogenic miRNAs miR-21 and miR-155; are deficient for p53-regulated miRNAs; and have heightened expression of miR-34 target genes such as Met and Bcl-2;MicroRNA 34a targets Bcl 2 and sensitizes human hepatocellular carcinoma cells to sorafenib treatment; HCC tissues with lower miR-34a expression displayed higher expression of Bcl-2 protein than those with high expression of miR-34a; therefore an inverse correlation is evident between the miR-34a level and Bcl-2 expression; Bioinformatics and luciferase reporter assays revealed that miR-34a binds the 3'-UTR of the Bcl-2 mRNA and represses its translation; Western blotting analysis and qRT-PCR confirmed that Bcl-2 is inhibited by miR-34a overexpression; Functional analyses indicated that the restoration of miR-34a reduced cell viability promoted cell apoptosis and potentiated sorafenib-induced apoptosis and toxicity in HCC cell lines by inhibiting Bcl-2 expression
49 hsa-miR-140-5p BCL2L1 -0.76 0 0.52 0 PITA; miRanda; miRNATAP -0.18 0 NA
50 hsa-miR-140-5p BCL9 -0.76 0 1.09 0 MirTarget; PITA; miRanda; miRNATAP -0.21 0 NA
51 hsa-miR-140-5p BMP8A -0.76 0 2.55 0 miRanda -0.17 0.00068 NA
52 hsa-miR-194-5p BNC1 0.47 0 -2.84 0 MirTarget -0.43 5.0E-5 NA
53 hsa-miR-34a-5p BNC2 0.13 0.13326 -0.78 0 miRNATAP -0.13 0.03676 NA
54 hsa-miR-140-5p BRD8 -0.76 0 0.07 0.22169 miRanda -0.11 0 NA
55 hsa-miR-194-5p BRMS1L 0.47 0 -0.44 0 MirTarget -0.12 0 NA
56 hsa-miR-34a-5p BRPF3 0.13 0.13326 0.09 0.20587 MirTarget; miRNATAP -0.12 3.0E-5 NA
57 hsa-miR-140-5p BSN -0.76 0 -0.24 0.13233 mirMAP -0.15 0.0064 NA
58 hsa-miR-194-5p BTBD7 0.47 0 -0.65 0 miRNATAP -0.11 0.0001 NA
59 hsa-miR-34a-5p C17orf51 0.13 0.13326 -1.38 0 mirMAP -0.18 0.00028 NA
60 hsa-miR-34a-5p C1orf21 0.13 0.13326 -0.47 0.00106 mirMAP -0.15 0.01067 NA
61 hsa-miR-152-3p CABP7 -0.27 0.00639 1.18 0 MirTarget -0.2 0.00095 NA
62 hsa-miR-34a-5p CACHD1 0.13 0.13326 -2.57 0 MirTarget -0.38 0 NA
63 hsa-miR-194-5p CADM1 0.47 0 -0.07 0.58588 miRNATAP -0.13 0.0115 NA
64 hsa-miR-140-5p CADPS -0.76 0 1.86 0 miRanda -0.29 2.0E-5 NA
65 hsa-miR-140-5p CAMK2N1 -0.76 0 0.27 0.07667 PITA; miRanda -0.29 0 NA
66 hsa-miR-140-5p CAPN1 -0.76 0 0.6 0 miRanda; miRNATAP -0.12 0 NA
67 hsa-miR-34a-5p CAPN6 0.13 0.13326 -3.62 0 miRNATAP -0.8 0 NA
68 hsa-miR-140-5p CARD10 -0.76 0 -0.48 6.0E-5 miRanda -0.13 0.00133 NA
69 hsa-miR-140-5p CASC1 -0.76 0 0.07 0.72877 miRanda -0.54 0 NA
70 hsa-miR-140-5p CBWD3 -0.76 0 0.89 0 miRanda -0.2 0 NA
71 hsa-miR-140-5p CC2D1A -0.76 0 0.42 0 miRanda -0.16 0 NA
72 hsa-miR-140-5p CCDC120 -0.76 0 0.74 0 miRanda -0.15 0 NA
73 hsa-miR-140-5p CCDC13 -0.76 0 -0.04 0.76305 miRanda -0.12 0.00611 NA
74 hsa-miR-140-5p CCDC30 -0.76 0 0.19 0.10313 miRanda -0.29 0 NA
75 hsa-miR-34a-5p CCDC50 0.13 0.13326 -1.33 0 miRNATAP -0.11 0.00025 NA
76 hsa-miR-140-5p CCDC78 -0.76 0 3.58 0 miRanda -0.82 0 NA
77 hsa-miR-140-5p CCNB1 -0.76 0 2.42 0 miRanda -0.15 0.00114 NA
78 hsa-miR-140-5p CCNF -0.76 0 1.72 0 miRanda -0.21 0 NA
79 hsa-miR-140-5p CD276 -0.76 0 0.96 0 miRanda -0.1 1.0E-5 NA
80 hsa-miR-194-5p CD302 0.47 0 -1.77 0 mirMAP -0.21 0 NA
81 hsa-miR-140-5p CD46 -0.76 0 0.22 0.00794 miRanda -0.14 0 NA
82 hsa-miR-194-5p CDC42BPA 0.47 0 -0.67 0 mirMAP -0.1 0.01633 NA
83 hsa-miR-140-5p CDHR3 -0.76 0 -0.46 0.04587 miRanda -0.2 0.01135 NA
84 hsa-miR-140-5p CDK1 -0.76 0 3.03 0 miRanda -0.14 0.01652 NA
85 hsa-miR-194-5p CDK14 0.47 0 -0.43 5.0E-5 MirTarget -0.17 6.0E-5 NA
86 hsa-miR-34a-5p CDKN1C 0.13 0.13326 -2.11 0 miRNATAP -0.28 0 NA
87 hsa-miR-140-5p CDKN3 -0.76 0 3.05 0 miRanda -0.14 0.01639 NA
88 hsa-miR-140-5p CEACAM6 -0.76 0 3.15 0 miRanda -0.72 0 NA
89 hsa-miR-34a-5p CELF2 0.13 0.13326 -2.12 0 miRNATAP -0.14 0.01317 NA
90 hsa-miR-140-5p CELF3 -0.76 0 2.06 0 miRanda -0.55 0 NA
91 hsa-miR-152-3p CELSR1 -0.27 0.00639 1.62 0 MirTarget -0.24 0.0001 NA
92 hsa-miR-152-3p CEP55 -0.27 0.00639 3.48 0 MirTarget -0.15 0.0288 NA
93 hsa-miR-140-5p CERCAM -0.76 0 1.06 0 miRanda; miRNATAP -0.25 0 NA
94 hsa-miR-194-5p CHD9 0.47 0 -1.21 0 miRNATAP -0.21 0 NA
95 hsa-miR-140-5p CHST15 -0.76 0 0.52 0 miRanda -0.23 0 NA
96 hsa-miR-194-5p CLDN19 0.47 0 -4.73 0 mirMAP -0.59 2.0E-5 NA
97 hsa-miR-140-5p CLEC5A -0.76 0 2.97 0 miRanda -0.14 0.03363 NA
98 hsa-miR-194-5p CLIP4 0.47 0 -2.32 0 MirTarget -0.21 0.00162 NA
99 hsa-miR-34a-5p CLOCK 0.13 0.13326 -0.44 0.00109 MirTarget -0.11 0.04508 NA
100 hsa-miR-34a-5p CNTN2 0.13 0.13326 -1.15 0 MirTarget; miRNATAP -0.27 0.00433 NA
101 hsa-miR-152-3p COL2A1 -0.27 0.00639 -0.25 0.56528 MirTarget -0.3 0.04906 NA
102 hsa-miR-140-5p COPG2 -0.76 0 0.25 0.00034 miRanda -0.13 0 NA
103 hsa-miR-140-5p COQ4 -0.76 0 0.53 0 miRanda -0.25 0 NA
104 hsa-miR-140-5p CORIN -0.76 0 2 0 miRanda -0.34 0 NA
105 hsa-miR-140-5p CORO2A -0.76 0 1.38 0 MirTarget; miRanda; miRNATAP -0.29 0 NA
106 hsa-miR-34a-5p CR2 0.13 0.13326 -0.58 0.1206 MirTarget -0.33 0.0271 NA
107 hsa-miR-140-5p CREB3L1 -0.76 0 1.98 0 MirTarget; miRanda; miRNATAP -0.45 0 NA
108 hsa-miR-140-5p CREB3L4 -0.76 0 1.73 0 miRanda -0.57 0 NA
109 hsa-miR-140-5p CROT -0.76 0 0.04 0.77707 miRNATAP -0.28 0 NA
110 hsa-miR-34a-5p CRY2 0.13 0.13326 -1.2 0 MirTarget -0.13 7.0E-5 NA
111 hsa-miR-140-5p CST9L -0.76 0 0.9 0.00141 miRanda -0.54 0 NA
112 hsa-miR-34a-5p CTDSP2 0.13 0.13326 -0.73 0 miRNATAP -0.14 0 NA
113 hsa-miR-194-5p CTDSPL 0.47 0 -0.59 0 mirMAP -0.14 0 NA
114 hsa-miR-140-5p CTSF -0.76 0 -0.45 5.0E-5 miRanda -0.15 4.0E-5 NA
115 hsa-miR-194-5p CTTNBP2NL 0.47 0 -0.5 0 MirTarget -0.11 9.0E-5 NA
116 hsa-miR-140-5p CUEDC1 -0.76 0 0.54 0 PITA; miRanda -0.19 0 NA
117 hsa-miR-140-5p CXXC4 -0.76 0 -0.35 0.08009 miRanda -0.13 0.04681 NA
118 hsa-miR-140-5p CYB5D1 -0.76 0 -0.14 0.07571 miRanda -0.15 0 NA
119 hsa-miR-194-5p CYP26B1 0.47 0 -2.18 0 mirMAP -0.17 0.01637 NA
120 hsa-miR-140-5p CYTH2 -0.76 0 0.7 0 MirTarget; miRanda; miRNATAP -0.27 0 NA
121 hsa-miR-194-5p CYTH3 0.47 0 -0.74 0 mirMAP -0.15 3.0E-5 NA
122 hsa-miR-194-5p DAAM1 0.47 0 -0.83 0 miRNATAP -0.19 4.0E-5 NA
123 hsa-miR-34a-5p DAPK2 0.13 0.13326 -1.03 0 MirTarget -0.1 0.02207 27704360 E2F 1 promotes DAPK2 induced anti tumor immunity of gastric cancer cells by targeting miR 34a
124 hsa-miR-194-5p DCBLD2 0.47 0 -0.92 0 MirTarget -0.2 0 NA
125 hsa-miR-140-5p DCDC1 -0.76 0 0.96 5.0E-5 PITA; miRanda -0.52 0 NA
126 hsa-miR-140-5p DCDC2 -0.76 0 -1.86 0 miRanda -0.2 0.0478 NA
127 hsa-miR-34a-5p DCX 0.13 0.13326 -2.75 0 MirTarget; miRNATAP -0.68 0 NA
128 hsa-miR-140-5p DHCR24 -0.76 0 0.77 0 miRanda -0.32 0 NA
129 hsa-miR-140-5p DHRS4L1 -0.76 0 -0.1 0.44303 miRanda -0.21 0 NA
130 hsa-miR-194-5p DISC1 0.47 0 -0.75 0 MirTarget -0.13 0.00032 NA
131 hsa-miR-34a-5p DLL1 0.13 0.13326 -0.95 0 miRTarBase; miRNATAP -0.18 0.0003 22438124 Delta tocotrienol suppresses Notch 1 pathway by upregulating miR 34a in nonsmall cell lung cancer cells
132 hsa-miR-194-5p DMD 0.47 0 -3.78 0 MirTarget; miRNATAP -0.37 1.0E-5 NA
133 hsa-miR-152-3p DMPK -0.27 0.00639 0.32 0.00202 MirTarget -0.16 1.0E-5 NA
134 hsa-miR-140-5p DNAH14 -0.76 0 1.8 0 miRanda -0.21 0 NA
135 hsa-miR-140-5p DNAH7 -0.76 0 -0.38 0.0511 miRanda -0.32 0 NA
136 hsa-miR-140-5p DNAJA4 -0.76 0 0.78 0 miRanda -0.22 0 NA
137 hsa-miR-140-5p DNMBP -0.76 0 -0.67 0 miRanda -0.1 0.01111 NA
138 hsa-miR-152-3p DNMT1 -0.27 0.00639 1.04 0 miRTarBase; MirTarget -0.16 0 27475839; 21868754; 25004396; 20578129; 24998573; 23318422 mRNA and protein expression profile of DNMT1 implicate that miR-152 targets DNMT1 mRNA and inhibits its protein expression; Further experiments revealed that DNMT1 plays crucial role for regulation of miR-152 gene; When DNMT1 protein function is blocked miR-152 expression prevails and destroys the mRNA of DNMT1; this molecular regulatory mechanism is creating a cyclic feedback loop which is now focused as DNMT1/miR-152 switch for on/off of DNMT1 target genes; We have demonstrated further that DNMT1 down regulation mediated upregulation of CDH1 hereafter DNMT1/CDH1 loop in presence of ectopic-excess of miR-152 prevents migration of cancer cells;Epigenetic silencing documented in miR-152 was consistent with its location at 17q21.32 in intron 1 of the COPZ2 gene which is also silenced often in endometrial cancer by DNA hypermethylation and also with evidence that miR-152 targets the DNA methyltransferase DNMT1;There appeared to be a reciprocal regulatory relationship of miR-152/DNMT1 expression as cells treated with siRNA DNMT1 caused miR-152 to be re-expressed in all cell lines;Our results showed that the expression of microRNA-152 miR-152 was frequently down-regulated in HBV-related HCC tissues in comparison with adjacent noncancerous hepatic tissues and was inversely correlated to DNA methyltransferase 1 DNMT1 messenger RNA mRNA expression in HBV-related HCCs; The forced expression of miR-152 in liver cell lines resulted in a marked reduction of the expression of DNMT1 at both the mRNA and protein levels by directly targeting the 3' untranslated regions of DNMT1; Our findings suggest that miR-152 is frequently down-regulated and regulates DNMT1 in HBV-related HCC;Furthermore Western blotting showed that the miR-152 mimic downregulated Wnt-1 DNMT1 ERK1/2 AKT and TNFRS6B signaling;MiR 152 and miR 185 co contribute to ovarian cancer cells cisplatin sensitivity by targeting DNMT1 directly: a novel epigenetic therapy independent of decitabine; The overexpression of miR-152 or miR-185 increased cisplatin sensitivity of SKOV3/DDP and A2780/DDP cells by inhibiting proliferation and promoting apoptosis then we further confirmed that these miRNAs functioned through suppressing DNA methyltransferase 1 DNMT1 directly; Our results indicated that miR-152 and miR-185 were involved in ovarian cancer cisplatin resistance in vitro and in vivo by targeting DNMT1 directly
139 hsa-miR-140-5p DNPEP -0.76 0 0.47 0 miRTarBase -0.16 0 NA
140 hsa-miR-140-5p DPP9 -0.76 0 0.84 0 miRanda -0.19 0 NA
141 hsa-miR-140-5p DUSP16 -0.76 0 -0 0.97223 miRNATAP -0.17 0 NA
142 hsa-miR-140-5p EARS2 -0.76 0 0.69 0 miRanda -0.2 0 NA
143 hsa-miR-140-5p ECHDC2 -0.76 0 -0.58 0 miRanda -0.16 1.0E-5 NA
144 hsa-miR-140-5p ECM1 -0.76 0 1.1 0 miRanda -0.31 0 NA
145 hsa-miR-34a-5p EDAR 0.13 0.13326 -2.79 0 miRNATAP -0.46 4.0E-5 NA
146 hsa-miR-194-5p EDN1 0.47 0 -1.72 0 MirTarget; miRNATAP -0.22 0.00072 NA
147 hsa-miR-140-5p EFCAB2 -0.76 0 -0.08 0.43842 miRanda -0.14 4.0E-5 NA
148 hsa-miR-140-5p EFHC2 -0.76 0 0.35 0.08676 miRanda -0.18 0.00997 NA
149 hsa-miR-140-5p EFNA4 -0.76 0 1.37 0 PITA; miRanda -0.17 0.0001 NA
150 hsa-miR-34a-5p EFNB3 0.13 0.13326 -1.46 0 miRNATAP -0.22 0.00215 NA

Over-represented Gene Ontology Biological Process

NumGOOverlapSizeP ValueAdj. P Value
1 INTRACELLULAR SIGNAL TRANSDUCTION 98 1572 2.991e-11 1.11e-07
2 NEUROGENESIS 90 1402 4.77e-11 1.11e-07
3 REGULATION OF INTRACELLULAR SIGNAL TRANSDUCTION 98 1656 5.232e-10 8.116e-07
4 REGULATION OF MULTICELLULAR ORGANISMAL DEVELOPMENT 98 1672 8.739e-10 1.017e-06
5 REGULATION OF CELL DIFFERENTIATION 88 1492 5.421e-09 5.044e-06
6 POSITIVE REGULATION OF CELL COMMUNICATION 87 1532 3.856e-08 2.99e-05
7 NEURON DIFFERENTIATION 58 874 5.84e-08 3.882e-05
8 POSITIVE REGULATION OF DEVELOPMENTAL GROWTH 20 156 9.504e-08 4.929e-05
9 REGULATION OF ANATOMICAL STRUCTURE MORPHOGENESIS 64 1021 9.533e-08 4.929e-05
10 CELL DEVELOPMENT 81 1426 1.2e-07 5.583e-05
11 REGULATION OF TRANSCRIPTION FROM RNA POLYMERASE II PROMOTER 95 1784 1.78e-07 7.531e-05
12 ORGAN MORPHOGENESIS 55 841 2.106e-07 7.538e-05
13 TISSUE DEVELOPMENT 84 1518 2.084e-07 7.538e-05
14 POSITIVE REGULATION OF DEVELOPMENTAL PROCESS 68 1142 2.532e-07 8.416e-05
15 REGULATION OF GROWTH 45 633 2.889e-07 8.625e-05
16 REGULATION OF MUSCLE CELL DIFFERENTIATION 19 152 2.966e-07 8.625e-05
17 REGULATION OF NEURON DIFFERENTIATION 41 554 3.477e-07 8.988e-05
18 CELL MORPHOGENESIS INVOLVED IN DIFFERENTIATION 39 513 3.425e-07 8.988e-05
19 REGULATION OF CELL DEVELOPMENT 54 836 4.032e-07 9.875e-05
20 REGULATION OF MEMBRANE POTENTIAL 30 343 4.436e-07 0.0001032
21 CELL MORPHOGENESIS INVOLVED IN NEURON DIFFERENTIATION 31 368 6.46e-07 0.0001431
22 POSITIVE REGULATION OF MULTICELLULAR ORGANISMAL PROCESS 77 1395 7.856e-07 0.0001624
23 CELLULAR COMPONENT MORPHOGENESIS 56 900 8.027e-07 0.0001624
24 REGULATION OF TRANSPORT 93 1804 1.025e-06 0.0001987
25 CENTRAL NERVOUS SYSTEM DEVELOPMENT 54 872 1.472e-06 0.0002739
26 REGULATION OF DEVELOPMENTAL GROWTH 26 289 1.532e-06 0.0002741
27 NEGATIVE REGULATION OF TRANSCRIPTION FROM RNA POLYMERASE II PROMOTER 48 740 1.622e-06 0.0002796
28 POSITIVE REGULATION OF GROWTH 23 238 1.822e-06 0.0003028
29 SENSORY ORGAN MORPHOGENESIS 23 239 1.958e-06 0.0003142
30 POSITIVE REGULATION OF GENE EXPRESSION 89 1733 2.123e-06 0.0003293
31 POSITIVE REGULATION OF CELL DIFFERENTIATION 51 823 2.858e-06 0.000429
32 EAR DEVELOPMENT 20 195 3.443e-06 0.0005007
33 CELL PART MORPHOGENESIS 42 633 4.216e-06 0.0005769
34 NEURON PROJECTION MORPHOGENESIS 31 402 4.126e-06 0.0005769
35 CEREBRAL CORTEX DEVELOPMENT 14 105 4.987e-06 0.0006446
36 PHOSPHATE CONTAINING COMPOUND METABOLIC PROCESS 97 1977 4.973e-06 0.0006446
37 REGULATION OF NERVOUS SYSTEM DEVELOPMENT 47 750 5.275e-06 0.0006459
38 RENAL TUBULE DEVELOPMENT 12 78 5.237e-06 0.0006459
39 REGULATION OF CELL PROLIFERATION 78 1496 5.675e-06 0.000677
40 NEURON DEVELOPMENT 44 687 6.142e-06 0.0006777
41 POSITIVE REGULATION OF BIOSYNTHETIC PROCESS 90 1805 6.12e-06 0.0006777
42 REGULATION OF CELLULAR LOCALIZATION 69 1277 6.409e-06 0.0006777
43 HEAD DEVELOPMENT 45 709 6.07e-06 0.0006777
44 SENSORY ORGAN DEVELOPMENT 35 493 6.342e-06 0.0006777
45 RESPONSE TO GROWTH FACTOR 34 475 7.219e-06 0.0007464
46 FOREBRAIN DEVELOPMENT 28 357 8.754e-06 0.0008313
47 EMBRYONIC ORGAN MORPHOGENESIS 24 279 8.284e-06 0.0008313
48 REGULATION OF HISTONE H3 K9 METHYLATION 6 17 8.46e-06 0.0008313
49 NEURON MIGRATION 14 110 8.651e-06 0.0008313
50 REGULATION OF CELLULAR COMPONENT MOVEMENT 47 771 1.086e-05 0.0009904
51 TELENCEPHALON DEVELOPMENT 21 228 1.071e-05 0.0009904
52 POSITIVE REGULATION OF MUSCLE CELL DIFFERENTIATION 12 84 1.149e-05 0.001016
53 POSITIVE REGULATION OF TRANSPORT 54 936 1.158e-05 0.001016
54 POSITIVE REGULATION OF HEART GROWTH 7 27 1.464e-05 0.001261
55 REGULATION OF MUSCLE HYPERTROPHY 8 37 1.523e-05 0.001289
56 GROWTH 30 410 1.633e-05 0.001357
57 IMMUNE SYSTEM DEVELOPMENT 38 582 1.735e-05 0.001416
58 MOVEMENT OF CELL OR SUBCELLULAR COMPONENT 67 1275 2.148e-05 0.001723
59 NEURON PROJECTION DEVELOPMENT 36 545 2.256e-05 0.001779
60 PHOSPHORYLATION 65 1228 2.301e-05 0.001784
61 PALLIUM DEVELOPMENT 16 153 2.531e-05 0.001931
62 MORPHOGENESIS OF AN EPITHELIUM 29 400 2.669e-05 0.002003
63 LOCOMOTION 60 1114 2.931e-05 0.002165
64 MODULATION OF EXCITATORY POSTSYNAPTIC POTENTIAL 7 30 3.093e-05 0.002248
65 NEGATIVE REGULATION OF NEURON DIFFERENTIATION 18 191 3.322e-05 0.002273
66 ENZYME LINKED RECEPTOR PROTEIN SIGNALING PATHWAY 42 689 3.209e-05 0.002273
67 NEPHRON EPITHELIUM DEVELOPMENT 12 93 3.279e-05 0.002273
68 POSITIVE REGULATION OF INTRACELLULAR SIGNAL TRANSDUCTION 50 876 3.25e-05 0.002273
69 POSITIVE REGULATION OF NEURON DIFFERENTIATION 24 306 3.791e-05 0.002556
70 ANATOMICAL STRUCTURE FORMATION INVOLVED IN MORPHOGENESIS 53 957 4.214e-05 0.002801
71 POSITIVE REGULATION OF RESPONSE TO STIMULUS 91 1929 4.566e-05 0.002951
72 CELLULAR RESPONSE TO ENDOGENOUS STIMULUS 55 1008 4.506e-05 0.002951
73 NEGATIVE REGULATION OF CELL DIFFERENTIATION 38 609 4.649e-05 0.002963
74 CELL FATE DETERMINATION 8 43 4.85e-05 0.003009
75 REGULATION OF NEURON DEATH 21 252 4.785e-05 0.003009
76 POSITIVE REGULATION OF PROTEIN METABOLIC PROCESS 74 1492 5.335e-05 0.003237
77 PROTEIN LOCALIZATION 86 1805 5.357e-05 0.003237
78 POSITIVE REGULATION OF MUSCLE TISSUE DEVELOPMENT 9 56 5.541e-05 0.003305
79 NEURON RECOGNITION 7 33 5.981e-05 0.003523
80 NEPHRON DEVELOPMENT 13 115 6.404e-05 0.003725
81 MUSCLE ORGAN DEVELOPMENT 22 277 6.557e-05 0.003766
82 REGULATION OF STRIATED MUSCLE CELL DIFFERENTIATION 11 85 6.754e-05 0.003819
83 CELL PROJECTION ORGANIZATION 50 902 6.812e-05 0.003819
84 HEART DEVELOPMENT 31 466 7.274e-05 0.004029
85 PIGMENTATION 11 86 7.529e-05 0.004122
86 POSITIVE REGULATION OF TRANSCRIPTION FROM RNA POLYMERASE II PROMOTER 54 1004 7.713e-05 0.004173
87 REGULATION OF CELL PROJECTION ORGANIZATION 35 558 8.314e-05 0.004437
88 NEURON PROJECTION GUIDANCE 18 205 8.391e-05 0.004437
89 EMBRYONIC ORGAN DEVELOPMENT 28 406 8.741e-05 0.00457
90 NEGATIVE REGULATION OF CELL DEVELOPMENT 23 303 8.994e-05 0.00465
91 REGULATION OF SECRETION 41 699 9.325e-05 0.00475
92 EMBRYONIC MORPHOGENESIS 34 539 9.392e-05 0.00475
93 REGULATION OF NEURON PROJECTION DEVELOPMENT 28 408 9.506e-05 0.004756
94 EMBRYO DEVELOPMENT 49 894 0.0001059 0.005188
95 MUSCLE STRUCTURE DEVELOPMENT 29 432 0.0001054 0.005188
96 PROTEIN PHOSPHORYLATION 51 944 0.0001096 0.00531
97 REGULATION OF PHOSPHATIDYLINOSITOL 3 KINASE SIGNALING 14 138 0.0001113 0.005316
98 RESPONSE TO ENDOGENOUS STIMULUS 71 1450 0.0001131 0.005316
99 NEGATIVE REGULATION OF CELL DEATH 48 872 0.0001125 0.005316
100 REGULATION OF PROTEIN LOCALIZATION 51 950 0.000128 0.005956
101 ANATOMICAL STRUCTURE ARRANGEMENT 5 17 0.0001338 0.006164
102 REGULATION OF MUSCLE ADAPTATION 9 63 0.0001425 0.006438
103 DEVELOPMENTAL GROWTH 24 333 0.0001418 0.006438
104 KIDNEY EPITHELIUM DEVELOPMENT 13 125 0.000151 0.006756
105 POSITIVE REGULATION OF ORGAN GROWTH 7 38 0.0001543 0.006837
106 CARDIOVASCULAR SYSTEM DEVELOPMENT 44 788 0.0001584 0.006888
107 CIRCULATORY SYSTEM DEVELOPMENT 44 788 0.0001584 0.006888
108 POSITIVE REGULATION OF PROTEIN MODIFICATION PROCESS 58 1135 0.000166 0.00715
109 TISSUE MORPHOGENESIS 33 533 0.0001678 0.007161
110 ACTION POTENTIAL 11 94 0.0001696 0.007175
111 DENDRITE DEVELOPMENT 10 79 0.0001732 0.007261
112 PEPTIDYL AMINO ACID MODIFICATION 46 841 0.0001804 0.007446
113 REGULATION OF PROTEIN MODIFICATION PROCESS 80 1710 0.0001808 0.007446
114 POSITIVE REGULATION OF MOLECULAR FUNCTION 83 1791 0.0001845 0.00753
115 NEGATIVE REGULATION OF CELL COMMUNICATION 60 1192 0.0001914 0.007745
116 POSITIVE REGULATION OF STRIATED MUSCLE CELL DIFFERENTIATION 8 52 0.0001965 0.007883
117 SINGLE ORGANISM BEHAVIOR 26 384 0.0002068 0.008154
118 FOREBRAIN GENERATION OF NEURONS 9 66 0.0002051 0.008154
119 POSITIVE REGULATION OF CELL DEVELOPMENT 30 472 0.0002101 0.008215
120 MODULATION OF SYNAPTIC TRANSMISSION 22 301 0.0002177 0.008443
121 PEPTIDYL SERINE MODIFICATION 14 148 0.0002336 0.008679
122 NEGATIVE REGULATION OF NERVOUS SYSTEM DEVELOPMENT 20 262 0.0002357 0.008679
123 CENTRAL NERVOUS SYSTEM NEURON DIFFERENTIATION 15 166 0.0002345 0.008679
124 POSITIVE REGULATION OF CARDIAC MUSCLE TISSUE DEVELOPMENT 6 29 0.0002357 0.008679
125 NEURONAL STEM CELL POPULATION MAINTENANCE 5 19 0.0002387 0.008679
126 TUBE MORPHOGENESIS 23 323 0.0002308 0.008679
127 TRANSMEMBRANE RECEPTOR PROTEIN TYROSINE KINASE SIGNALING PATHWAY 31 498 0.0002387 0.008679
128 CEREBRAL CORTEX RADIALLY ORIENTED CELL MIGRATION 6 29 0.0002357 0.008679
129 EYE DEVELOPMENT 23 326 0.0002636 0.009509
NumGOOverlapSizeP ValueAdj. P Value
1 ENZYME BINDING 97 1737 1.289e-08 1.197e-05
2 TRANSCRIPTION FACTOR ACTIVITY RNA POLYMERASE II CORE PROMOTER PROXIMAL REGION SEQUENCE SPECIFIC BINDING 29 328 5.516e-07 0.0002562
3 REGULATORY REGION NUCLEIC ACID BINDING 51 818 2.402e-06 0.0005834
4 KINASE ACTIVITY 52 842 2.512e-06 0.0005834
5 RNA POLYMERASE II TRANSCRIPTION FACTOR ACTIVITY SEQUENCE SPECIFIC DNA BINDING 42 629 3.601e-06 0.000669
6 NUCLEIC ACID BINDING TRANSCRIPTION FACTOR ACTIVITY 64 1199 2.068e-05 0.003201
7 TRANSMEMBRANE RECEPTOR PROTEIN TYROSINE KINASE ACTIVITY 10 64 2.772e-05 0.003389
8 TRANSFERASE ACTIVITY TRANSFERRING PHOSPHORUS CONTAINING GROUPS 55 992 2.918e-05 0.003389
9 TRANSMEMBRANE RECEPTOR PROTEIN KINASE ACTIVITY 11 81 4.297e-05 0.004435
10 PROTEIN KINASE ACTIVITY 39 640 6.176e-05 0.005737
11 DOUBLE STRANDED DNA BINDING 44 764 7.909e-05 0.006679
12 TRANSCRIPTIONAL ACTIVATOR ACTIVITY RNA POLYMERASE II CORE PROMOTER PROXIMAL REGION SEQUENCE SPECIFIC BINDING 19 226 9.633e-05 0.007457
NumGOOverlapSizeP ValueAdj. P Value
1 CELL JUNCTION 71 1151 3.374e-08 1.971e-05
2 PLASMA MEMBRANE REGION 60 929 8.839e-08 2.581e-05
3 MEMBRANE REGION 65 1134 1.765e-06 0.0003436
4 NEURON PROJECTION 55 942 6.787e-06 0.0008303
5 CELL PROJECTION 89 1786 7.109e-06 0.0008303
6 NEURON PART 68 1265 8.91e-06 0.0008672
7 APICAL PART OF CELL 28 361 1.077e-05 0.0008985
8 SYNAPSE 46 754 1.322e-05 0.0009652
9 APICAL PLASMA MEMBRANE 24 292 1.771e-05 0.001149
10 CELL CELL JUNCTION 28 383 3.157e-05 0.001843
11 SOMATODENDRITIC COMPARTMENT 39 650 8.585e-05 0.004558
12 BASOLATERAL PLASMA MEMBRANE 18 211 0.0001213 0.005902
13 APICAL JUNCTION COMPLEX 13 128 0.0001917 0.008612

Over-represented Pathway

NumPathwayPathviewOverlapSizeP ValueAdj. P Value
1 Wnt_signaling_pathway_hsa04310 12 146 0.002169 0.04413
2 MAPK_signaling_pathway_hsa04010 19 295 0.002505 0.04413
3 Hippo_signaling_pathway_hsa04390 12 154 0.003371 0.04413
4 Apoptosis_multiple_species_hsa04215 5 33 0.003394 0.04413
5 Cellular_senescence_hsa04218 12 160 0.004585 0.04679
6 Rap1_signaling_pathway_hsa04015 14 206 0.005545 0.04679
7 Gap_junction_hsa04540 8 88 0.006299 0.04679
8 PI3K_Akt_signaling_pathway_hsa04151 20 352 0.007855 0.05106
9 Hippo_signaling_pathway_multiple_species_hsa04392 4 29 0.01213 0.07007
10 Ras_signaling_pathway_hsa04014 14 232 0.01485 0.07228
11 Hedgehog_signaling_pathway_hsa04340 5 47 0.01535 0.07228
12 Phospholipase_D_signaling_pathway_hsa04072 10 146 0.01668 0.07228
13 Focal_adhesion_hsa04510 12 199 0.02313 0.0925
14 Apoptosis_hsa04210 9 138 0.02967 0.1062
15 Signaling_pathways_regulating_pluripotency_of_stem_cells_hsa04550 9 139 0.03088 0.1062
16 cGMP_PKG_signaling_pathway_hsa04022 10 163 0.03267 0.1062
17 Phosphatidylinositol_signaling_system_hsa04070 7 99 0.03581 0.1095
18 Oocyte_meiosis_hsa04114 8 124 0.04119 0.119
19 Peroxisome_hsa04146 6 83 0.04573 0.1252
20 p53_signaling_pathway_hsa04115 5 68 0.06162 0.1602
21 Apelin_signaling_pathway_hsa04371 8 137 0.06637 0.1643
22 Adherens_junction_hsa04520 5 72 0.07492 0.1754
23 NF_kappa_B_signaling_pathway_hsa04064 6 95 0.07759 0.1754
24 AMPK_signaling_pathway_hsa04152 7 121 0.08581 0.1859
25 HIF_1_signaling_pathway_hsa04066 6 100 0.0938 0.1951
26 mTOR_signaling_pathway_hsa04150 8 151 0.1022 0.2045
27 Autophagy_animal_hsa04140 7 128 0.1073 0.2067
28 Calcium_signaling_pathway_hsa04020 9 182 0.1187 0.2204
29 ErbB_signaling_pathway_hsa04012 5 85 0.1277 0.229
30 Endocytosis_hsa04144 11 244 0.1451 0.2515
31 Cell_adhesion_molecules_.CAMs._hsa04514 7 145 0.1702 0.2854
32 Notch_signaling_pathway_hsa04330 3 48 0.1896 0.308
33 Jak_STAT_signaling_pathway_hsa04630 7 162 0.2454 0.3823
34 TNF_signaling_pathway_hsa04668 5 108 0.25 0.3823
35 Tight_junction_hsa04530 7 170 0.2839 0.4218
36 FoxO_signaling_pathway_hsa04068 5 132 0.3973 0.5739
37 cAMP_signaling_pathway_hsa04024 7 198 0.426 0.5987
38 ECM_receptor_interaction_hsa04512 3 82 0.4757 0.651
39 TGF_beta_signaling_pathway_hsa04350 3 84 0.4917 0.6556
40 Sphingolipid_signaling_pathway_hsa04071 4 118 0.5072 0.6593
41 Cell_cycle_hsa04110 4 124 0.5463 0.6874
42 VEGF_signaling_pathway_hsa04370 2 59 0.5552 0.6874
43 Necroptosis_hsa04217 5 164 0.5864 0.7076
44 Mitophagy_animal_hsa04137 2 65 0.6081 0.7076
45 Cytokine_cytokine_receptor_interaction_hsa04060 8 270 0.6123 0.7076
46 Lysosome_hsa04142 3 123 0.7446 0.8066
47 Regulation_of_actin_cytoskeleton_hsa04810 5 208 0.7834 0.8147
48 Neuroactive_ligand_receptor_interaction_hsa04080 6 278 0.8707 0.8878
49 Phagosome_hsa04145 2 152 0.9535 0.9535

Quest ID: 841e3a9f87240b6d39e50fc92d26230e